AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280006737.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280006737.01.T01 MTR_2g013950 85.827 254 13 5 1 239 7 252 3.53e-131 371
MsG0280006737.01.T01 MTR_4g088695 67.347 196 48 9 63 246 50 241 6.77e-64 200
MsG0280006737.01.T01 MTR_2g079120 56.219 201 60 7 59 240 51 242 8.09e-53 171
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280006737.01.T01 AT1G28280 57.025 242 62 11 32 240 7 239 7.41e-66 205
MsG0280006737.01.T01 AT1G28280 56.846 241 62 11 32 239 7 238 2.83e-65 203
MsG0280006737.01.T01 AT3G15300 50.249 201 64 12 63 240 29 216 5.11e-38 132
MsG0280006737.01.T01 AT5G53830 52.970 202 54 12 70 237 45 239 8.02e-38 133
MsG0280006737.01.T01 AT2G33780 44.444 189 82 6 64 243 26 200 4.09e-34 122
MsG0280006737.01.T01 AT1G80450 36.842 171 84 8 74 240 15 165 8.36e-14 68.2
MsG0280006737.01.T01 AT5G08480 36.842 171 89 7 74 241 17 171 4.16e-11 60.5
MsG0280006737.01.T01 AT5G08480 36.842 171 89 7 74 241 17 171 4.16e-11 60.5

Find 62 sgRNAs with CRISPR-Local

Find 74 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAACAAATGTTGTTGGATA+TGG 0.219375 2:-5593113 None:intergenic
CAAGAACCGGATCGGTTAAA+TGG 0.220372 2:-5593440 None:intergenic
GAAACTTGAGTTGGAGAAAA+TGG 0.246762 2:-5593327 None:intergenic
ACCTGGTTCGGATCTTGTTA+TGG 0.250404 2:-5593087 None:intergenic
CCTGGTTCGGATCTTGTTAT+GGG 0.271576 2:-5593086 None:intergenic
TGCTAGAAATGGTTCTTCTT+TGG 0.294813 2:+5593469 MsG0280006737.01.T01:CDS
CAAGTTGTTCAAATGTTAAC+TGG 0.300641 2:+5593158 MsG0280006737.01.T01:CDS
TGGGTCTTGGTGAAGGAATT+TGG 0.302573 2:-5593013 None:intergenic
ACTACTTCTCCTAGGGCTTC+AGG 0.309005 2:+5593602 MsG0280006737.01.T01:CDS
GAGAAAATGGGTGGTAATAA+AGG 0.316316 2:-5593314 None:intergenic
TTGTGTCCATTACTTGTTAT+GGG 0.319171 2:-5593032 None:intergenic
GTTGTGTCCATTACTTGTTA+TGG 0.323237 2:-5593033 None:intergenic
TCGGGTATGAGTGGTGTAAC+CGG 0.325510 2:-5593416 None:intergenic
CCATTACTTGTTATGGGTCT+TGG 0.332135 2:-5593026 None:intergenic
TGGGTCTTGGTGATTGAATT+TGG 0.337028 2:-5593067 None:intergenic
GTAATAAAGGGTTAATGTTA+AGG 0.349071 2:-5593301 None:intergenic
TCGGATCTTGTTATGGGTCT+TGG 0.376668 2:-5593080 None:intergenic
TTGGTGGAATGTTATGGTTA+TGG 0.382884 2:-5593226 None:intergenic
GATAAAGCTATAAGAGAAAA+GGG 0.402366 2:+5593506 MsG0280006737.01.T01:CDS
TTGGTGATTGAATTTGGTTA+TGG 0.403350 2:-5593061 None:intergenic
GTCTCTAGGAGTTGAAGCTC+TGG 0.403528 2:-5593549 None:intergenic
ATTGAATTTGGTTATGGCTA+TGG 0.404356 2:-5593055 None:intergenic
TCTTGTTCTGCTGCTAGAAA+TGG 0.406064 2:+5593458 MsG0280006737.01.T01:CDS
TGGATATGGGTTACCTGGTT+CGG 0.412484 2:-5593099 None:intergenic
GAAGGAAAATCTAGGATACT+AGG 0.429083 2:-5593380 None:intergenic
AAGGAATTTGGTTATGGCTA+TGG 0.434064 2:-5593001 None:intergenic
TTGGTGAAGGAATTTGGTTA+TGG 0.445585 2:-5593007 None:intergenic
AGATAAAGCTATAAGAGAAA+AGG 0.449288 2:+5593505 MsG0280006737.01.T01:CDS
GAACAAATGTTGTTGGATAT+GGG 0.451854 2:-5593112 None:intergenic
GAGCAAGAGAAGGAAAATCT+AGG 0.454989 2:-5593388 None:intergenic
TCCTTCTCTTGCTCTTAGCC+CGG 0.462751 2:+5593397 MsG0280006737.01.T01:CDS
AAGAACCGGATCGGTTAAAT+GGG 0.472120 2:-5593439 None:intergenic
GGAATGTTATGGTTATGGTT+TGG 0.475070 2:-5593221 None:intergenic
TCAGCTTGAACAAATGTTGT+TGG 0.479156 2:-5593119 None:intergenic
CCGGATCGGTTAAATGGGTC+GGG 0.490143 2:-5593434 None:intergenic
AAGGAAAATCTAGGATACTA+GGG 0.495004 2:-5593379 None:intergenic
AGAAAATGGGTGGTAATAAA+GGG 0.497203 2:-5593313 None:intergenic
CCCGACCCATTTAACCGATC+CGG 0.503223 2:+5593434 MsG0280006737.01.T01:CDS
CCAAGACCCATAACAAGTAA+TGG 0.503842 2:+5593026 MsG0280006737.01.T01:CDS
TCTAGCAGCAGAACAAGAAC+CGG 0.505923 2:-5593453 None:intergenic
CTTGTTATGGGTCTTGGTGA+AGG 0.506753 2:-5593020 None:intergenic
TCAAATGTTAACTGGTTCCT+CGG 0.513372 2:+5593166 MsG0280006737.01.T01:CDS
GGAGTTGAAGCTCTGGGAGA+AGG 0.513871 2:-5593542 None:intergenic
CGGGTATGAGTGGTGTAACC+GGG 0.519254 2:-5593415 None:intergenic
ACCGGATCGGTTAAATGGGT+CGG 0.532624 2:-5593435 None:intergenic
AAACTTGAGTTGGAGAAAAT+GGG 0.542299 2:-5593326 None:intergenic
GTTGTTGGATATGGGTTACC+TGG 0.543727 2:-5593104 None:intergenic
GAAGCCCTAGGAGAAGTAGT+AGG 0.571572 2:-5593599 None:intergenic
CAAACTCAAACTCAAAGCAA+TGG 0.576732 2:+5592972 MsG0280006737.01.T01:CDS
GATGAAGGACCTGAAGCCCT+AGG 0.577284 2:-5593611 None:intergenic
TCTCTAGGAGTTGAAGCTCT+GGG 0.580776 2:-5593548 None:intergenic
AAACGAGGTTCAGAGTCTCT+AGG 0.581466 2:-5593563 None:intergenic
ACGAGGTGAGAAACTTGAGT+TGG 0.589923 2:-5593336 None:intergenic
CCCATAACAAGATCCGAACC+AGG 0.590432 2:+5593086 MsG0280006737.01.T01:CDS
TTAAATGGGTCGGGTATGAG+TGG 0.616779 2:-5593425 None:intergenic
CAGCAGAACAAGAACCGGAT+CGG 0.628054 2:-5593448 None:intergenic
TTGAACAACTTGTTTGAACG+AGG 0.648447 2:-5593147 None:intergenic
AGGAAAATCTAGGATACTAG+GGG 0.652374 2:-5593378 None:intergenic
AAGATCTCTTGTTTGTTACG+AGG 0.652785 2:-5593353 None:intergenic
ACCGGGCTAAGAGCAAGAGA+AGG 0.662342 2:-5593398 None:intergenic
CTTGAGTTGGAGAAAATGGG+TGG 0.666617 2:-5593323 None:intergenic
ATCATCAATGAGATACCAAG+AGG 0.685459 2:+5592896 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AGATAAAGCTATAAGAGAAA+AGG + Chr2:5593505-5593524 MsG0280006737.01.T01:CDS 25.0%
! GATAAAGCTATAAGAGAAAA+GGG + Chr2:5593506-5593525 MsG0280006737.01.T01:CDS 25.0%
! GTAATAAAGGGTTAATGTTA+AGG - Chr2:5593304-5593323 None:intergenic 25.0%
!!! GAATATTTTGAAGAAGATGA+AGG - Chr2:5593629-5593648 None:intergenic 25.0%
!!! TTGAAACCTTGTTGTTTTTT+TGG - Chr2:5593248-5593267 None:intergenic 25.0%
AGAAAATGGGTGGTAATAAA+GGG - Chr2:5593316-5593335 None:intergenic 30.0%
ATTCCACCAAAAAAACAACA+AGG + Chr2:5593239-5593258 MsG0280006737.01.T01:CDS 30.0%
ATTGAATTTGGTTATGGCTA+TGG - Chr2:5593058-5593077 None:intergenic 30.0%
CAAGTTGTTCAAATGTTAAC+TGG + Chr2:5593158-5593177 MsG0280006737.01.T01:CDS 30.0%
GAACAAATGTTGTTGGATAT+GGG - Chr2:5593115-5593134 None:intergenic 30.0%
TGAACAAATGTTGTTGGATA+TGG - Chr2:5593116-5593135 None:intergenic 30.0%
! AAGGAAAATCTAGGATACTA+GGG - Chr2:5593382-5593401 None:intergenic 30.0%
! TTGGTGATTGAATTTGGTTA+TGG - Chr2:5593064-5593083 None:intergenic 30.0%
!! AAACTTGAGTTGGAGAAAAT+GGG - Chr2:5593329-5593348 None:intergenic 30.0%
!! TGAGTTTGAGTTTGAGTTTT+TGG - Chr2:5592954-5592973 None:intergenic 30.0%
!! TTGTGTCCATTACTTGTTAT+GGG - Chr2:5593035-5593054 None:intergenic 30.0%
!!! AAACCTTGTTGTTTTTTTGG+TGG - Chr2:5593245-5593264 None:intergenic 30.0%
!!! GTTTTTTTGGTGGAATGTTA+TGG - Chr2:5593235-5593254 None:intergenic 30.0%
AAGGAATTTGGTTATGGCTA+TGG - Chr2:5593004-5593023 None:intergenic 35.0%
CAAACTCAAACTCAAAGCAA+TGG + Chr2:5592972-5592991 MsG0280006737.01.T01:CDS 35.0%
GAGAAAATGGGTGGTAATAA+AGG - Chr2:5593317-5593336 None:intergenic 35.0%
GGAATGTTATGGTTATGGTT+TGG - Chr2:5593224-5593243 None:intergenic 35.0%
TCAGCTTGAACAAATGTTGT+TGG - Chr2:5593122-5593141 None:intergenic 35.0%
TGCTAGAAATGGTTCTTCTT+TGG + Chr2:5593469-5593488 MsG0280006737.01.T01:CDS 35.0%
TTGGTGAAGGAATTTGGTTA+TGG - Chr2:5593010-5593029 None:intergenic 35.0%
TTGGTGGAATGTTATGGTTA+TGG - Chr2:5593229-5593248 None:intergenic 35.0%
! AAGATCTCTTGTTTGTTACG+AGG - Chr2:5593356-5593375 None:intergenic 35.0%
! AGGAAAATCTAGGATACTAG+GGG - Chr2:5593381-5593400 None:intergenic 35.0%
! GAAGGAAAATCTAGGATACT+AGG - Chr2:5593383-5593402 None:intergenic 35.0%
! GAGATGATTTTTGTTCCTCT+TGG - Chr2:5592914-5592933 None:intergenic 35.0%
! GTTGTGTCCATTACTTGTTA+TGG - Chr2:5593036-5593055 None:intergenic 35.0%
! TCAAATGTTAACTGGTTCCT+CGG + Chr2:5593166-5593185 MsG0280006737.01.T01:CDS 35.0%
! TTGAACAACTTGTTTGAACG+AGG - Chr2:5593150-5593169 None:intergenic 35.0%
! TTTTCCTACTACTTCTCCTA+GGG + Chr2:5593595-5593614 MsG0280006737.01.T01:CDS 35.0%
! TTTTTCCTACTACTTCTCCT+AGG + Chr2:5593594-5593613 MsG0280006737.01.T01:CDS 35.0%
!! GAAACTTGAGTTGGAGAAAA+TGG - Chr2:5593330-5593349 None:intergenic 35.0%
!!! TGAGTTTGAGTTTTTGGTGA+AGG - Chr2:5592948-5592967 None:intergenic 35.0%
AAGAACCGGATCGGTTAAAT+GGG - Chr2:5593442-5593461 None:intergenic 40.0%
CCAAGACCCATAACAAGTAA+TGG + Chr2:5593026-5593045 MsG0280006737.01.T01:CDS 40.0%
GAGCAAGAGAAGGAAAATCT+AGG - Chr2:5593391-5593410 None:intergenic 40.0%
GGAGAAGTAGTAGGAAAAAG+AGG - Chr2:5593593-5593612 None:intergenic 40.0%
TCTTGTTCTGCTGCTAGAAA+TGG + Chr2:5593458-5593477 MsG0280006737.01.T01:CDS 40.0%
! TGGGTCTTGGTGATTGAATT+TGG - Chr2:5593070-5593089 None:intergenic 40.0%
!! CCATTACTTGTTATGGGTCT+TGG - Chr2:5593029-5593048 None:intergenic 40.0%
AAACGAGGTTCAGAGTCTCT+AGG - Chr2:5593566-5593585 None:intergenic 45.0%
ACGAGGTGAGAAACTTGAGT+TGG - Chr2:5593339-5593358 None:intergenic 45.0%
CAAGAACCGGATCGGTTAAA+TGG - Chr2:5593443-5593462 None:intergenic 45.0%
GGAAAAAGAGGAAGCAAACG+AGG - Chr2:5593581-5593600 None:intergenic 45.0%
TCTAGCAGCAGAACAAGAAC+CGG - Chr2:5593456-5593475 None:intergenic 45.0%
TGGGTCTTGGTGAAGGAATT+TGG - Chr2:5593016-5593035 None:intergenic 45.0%
TTAAATGGGTCGGGTATGAG+TGG - Chr2:5593428-5593447 None:intergenic 45.0%
! CTTGTTATGGGTCTTGGTGA+AGG - Chr2:5593023-5593042 None:intergenic 45.0%
! GTTGTTGGATATGGGTTACC+TGG - Chr2:5593107-5593126 None:intergenic 45.0%
! TCGGATCTTGTTATGGGTCT+TGG - Chr2:5593083-5593102 None:intergenic 45.0%
! TCTCTAGGAGTTGAAGCTCT+GGG - Chr2:5593551-5593570 None:intergenic 45.0%
! TGGATATGGGTTACCTGGTT+CGG - Chr2:5593102-5593121 None:intergenic 45.0%
!! ACCTGGTTCGGATCTTGTTA+TGG - Chr2:5593090-5593109 None:intergenic 45.0%
!! CCTGGTTCGGATCTTGTTAT+GGG - Chr2:5593089-5593108 None:intergenic 45.0%
!! CTTGAGTTGGAGAAAATGGG+TGG - Chr2:5593326-5593345 None:intergenic 45.0%
ACCGGATCGGTTAAATGGGT+CGG - Chr2:5593438-5593457 None:intergenic 50.0%
ACTACTTCTCCTAGGGCTTC+AGG + Chr2:5593602-5593621 MsG0280006737.01.T01:CDS 50.0%
CAGCAGAACAAGAACCGGAT+CGG - Chr2:5593451-5593470 None:intergenic 50.0%
CCCATAACAAGATCCGAACC+AGG + Chr2:5593086-5593105 MsG0280006737.01.T01:CDS 50.0%
GAAGCCCTAGGAGAAGTAGT+AGG - Chr2:5593602-5593621 None:intergenic 50.0%
TCCTTCTCTTGCTCTTAGCC+CGG + Chr2:5593397-5593416 MsG0280006737.01.T01:CDS 50.0%
TCGGGTATGAGTGGTGTAAC+CGG - Chr2:5593419-5593438 None:intergenic 50.0%
! GTCTCTAGGAGTTGAAGCTC+TGG - Chr2:5593552-5593571 None:intergenic 50.0%
!!! AGCTTGTTTTGCTGTCTCCG+AGG - Chr2:5593186-5593205 None:intergenic 50.0%
ACCGGGCTAAGAGCAAGAGA+AGG - Chr2:5593401-5593420 None:intergenic 55.0%
CCCGACCCATTTAACCGATC+CGG + Chr2:5593434-5593453 MsG0280006737.01.T01:CDS 55.0%
CCGGATCGGTTAAATGGGTC+GGG - Chr2:5593437-5593456 None:intergenic 55.0%
CGGGTATGAGTGGTGTAACC+GGG - Chr2:5593418-5593437 None:intergenic 55.0%
GATGAAGGACCTGAAGCCCT+AGG - Chr2:5593614-5593633 None:intergenic 55.0%
!! GGAGTTGAAGCTCTGGGAGA+AGG - Chr2:5593545-5593564 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr2 gene 5592903 5593658 5592903 ID=MsG0280006737.01;Name=MsG0280006737.01
Chr2 mRNA 5592903 5593658 5592903 ID=MsG0280006737.01.T01;Parent=MsG0280006737.01;Name=MsG0280006737.01.T01;_AED=0.39;_eAED=0.39;_QI=0|-1|0|1|-1|1|1|0|251
Chr2 exon 5592903 5593658 5592903 ID=MsG0280006737.01.T01:exon:2278;Parent=MsG0280006737.01.T01
Chr2 CDS 5592903 5593658 5592903 ID=MsG0280006737.01.T01:cds;Parent=MsG0280006737.01.T01
Gene Sequence

>MsG0280006737.01.T01

ATGAGATACCAAGAGGAACAAAAATCATCTCTCACTTCACTACCTTCACCAAAAACTCAAACTCAAACTCAAACTCAAACTCAAAGCAATGGACACAACCATAGCCATAACCAAATTCCTTCACCAAGACCCATAACAAGTAATGGACACAACCATAGCCATAACCAAATTCAATCACCAAGACCCATAACAAGATCCGAACCAGGTAACCCATATCCAACAACATTTGTTCAAGCTGACACAACCTCGTTCAAACAAGTTGTTCAAATGTTAACTGGTTCCTCGGAGACAGCAAAACAAGCTTCAAGTTCAACAAAACCAAACCATAACCATAACATTCCACCAAAAAAACAACAAGGTTTCAAACTCTATGAAAGAAGAAACTCATTTCACAAAAACCTTAACATTAACCCTTTATTACCACCCATTTTCTCCAACTCAAGTTTCTCACCTCGTAACAAACAAGAGATCTTGTCCCCTAGTATCCTAGATTTTCCTTCTCTTGCTCTTAGCCCGGTTACACCACTCATACCCGACCCATTTAACCGATCCGGTTCTTGTTCTGCTGCTAGAAATGGTTCTTCTTTGGATTCTTTAGCTGAAGATAAAGCTATAAGAGAAAAGGGTTTCTTTCTGCATCCTTCTCCCAGAGCTTCAACTCCTAGAGACTCTGAACCTCGTTTGCTTCCTCTTTTTCCTACTACTTCTCCTAGGGCTTCAGGTCCTTCATCTTCTTCAAAATATTCTGCATCTTGA

Protein sequence

>MsG0280006737.01.T01

MRYQEEQKSSLTSLPSPKTQTQTQTQTQSNGHNHSHNQIPSPRPITSNGHNHSHNQIQSPRPITRSEPGNPYPTTFVQADTTSFKQVVQMLTGSSETAKQASSSTKPNHNHNIPPKKQQGFKLYERRNSFHKNLNINPLLPPIFSNSSFSPRNKQEILSPSILDFPSLALSPVTPLIPDPFNRSGSCSAARNGSSLDSLAEDKAIREKGFFLHPSPRASTPRDSEPRLLPLFPTTSPRASGPSSSSKYSAS*