AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780037987.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780037987.01.T01 MTR_4g006040 96.774 93 3 0 6 98 145 237 9.44e-62 187
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 40 sgRNAs with CRISPR-Local

Find 41 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGAGATCACCTCTTTCATTC+TGG 0.220450 7:-37742150 MsG0780037987.01.T01:CDS
TCGTGGGATCCGGGCGGTCC+AGG 0.255275 7:-37742323 MsG0780037987.01.T01:CDS
GAGGATAGATGGGGCGATTT+AGG 0.255544 7:-37742379 MsG0780037987.01.T01:CDS
TTCTATTCACTCGTGGGATC+CGG 0.285661 7:-37742333 MsG0780037987.01.T01:CDS
ATATATCCCTTATCAGTATA+TGG 0.331456 7:-37742265 MsG0780037987.01.T01:CDS
CTTCTCCTCAGGAGGATAGA+TGG 0.346630 7:-37742390 MsG0780037987.01.T01:CDS
TATCCTCCTGAGGAGAAGTT+TGG 0.356300 7:+37742395 None:intergenic
GAGATCACCTCTTTCATTCT+GGG 0.384850 7:-37742149 MsG0780037987.01.T01:CDS
TACCTCTCGAGCACAGGTTT+AGG 0.385731 7:-37742237 MsG0780037987.01.T01:CDS
GTGGGATCCGGGCGGTCCAG+GGG 0.385871 7:-37742321 MsG0780037987.01.T01:CDS
TGGGATCCGGGCGGTCCAGG+GGG 0.394291 7:-37742320 MsG0780037987.01.T01:CDS
CTCGAGCACAGGTTTAGGTT+TGG 0.402364 7:-37742232 MsG0780037987.01.T01:CDS
CGTGGGATCCGGGCGGTCCA+GGG 0.409711 7:-37742322 MsG0780037987.01.T01:CDS
AGAGGTGATCTCGTAGTTCT+TGG 0.431705 7:+37742160 None:intergenic
TTCTCCTCAGGAGGATAGAT+GGG 0.436019 7:-37742389 MsG0780037987.01.T01:CDS
TTTCATTCTGGGGTGACGGC+GGG 0.437684 7:-37742138 MsG0780037987.01.T01:CDS
TAGCTGTCCATATACTGATA+AGG 0.448263 7:+37742258 None:intergenic
TTGAAACCAAACTTCTCCTC+AGG 0.463068 7:-37742401 None:intergenic
AGCTGTCCATATACTGATAA+GGG 0.470075 7:+37742259 None:intergenic
TCTATTCACTCGTGGGATCC+GGG 0.481377 7:-37742332 MsG0780037987.01.T01:CDS
GTCTGTGAAGATACGTTGTT+AGG 0.494777 7:+37742182 None:intergenic
GGGATCCGGGCGGTCCAGGG+GGG 0.502113 7:-37742319 MsG0780037987.01.T01:CDS
CCCACGAGTGAATAGAAAGT+TGG 0.515787 7:+37742339 None:intergenic
TCCAACTTTCTATTCACTCG+TGG 0.529895 7:-37742340 MsG0780037987.01.T01:CDS
CTTTCATTCTGGGGTGACGG+CGG 0.540721 7:-37742139 MsG0780037987.01.T01:CDS
GACAGCTACCTCTCGAGCAC+AGG 0.545129 7:-37742243 MsG0780037987.01.T01:CDS
CGAGAGGAGAGGAGCCATGA+TGG 0.546958 7:+37742295 None:intergenic
CCGTCACCCCAGAATGAAAG+AGG 0.549518 7:+37742142 None:intergenic
CCTCTTTCATTCTGGGGTGA+CGG 0.551798 7:-37742142 MsG0780037987.01.T01:CDS
CCCATTTCGAGTCAAGAGAA+TGG 0.558119 7:+37742109 None:intergenic
ATAGAAAGTTGGATCTACAT+TGG 0.570959 7:+37742350 None:intergenic
AAACCAAACTTCTCCTCAGG+AGG 0.577461 7:-37742398 None:intergenic
CCAACTTTCTATTCACTCGT+GGG 0.591847 7:-37742339 MsG0780037987.01.T01:CDS
AACCTAAACCTGTGCTCGAG+AGG 0.607627 7:+37742235 None:intergenic
AGATCACCTCTTTCATTCTG+GGG 0.626680 7:-37742148 MsG0780037987.01.T01:CDS
ATATGAATTCTCGAGAGGAG+AGG 0.651842 7:+37742284 None:intergenic
GGGATATATGAATTCTCGAG+AGG 0.654907 7:+37742279 None:intergenic
TCGCCCCATCTATCCTCCTG+AGG 0.661903 7:+37742385 None:intergenic
TCTCCTCAGGAGGATAGATG+GGG 0.682857 7:-37742388 MsG0780037987.01.T01:CDS
ATTCACTCGTGGGATCCGGG+CGG 0.763324 7:-37742329 MsG0780037987.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! ATATATCCCTTATCAGTATA+TGG - Chr7:37742249-37742268 MsG0780037987.01.T01:CDS 25.0%
! CCTCAATAGAAAAAGAAAAA+CGG - Chr7:37742305-37742324 MsG0780037987.01.T01:CDS 25.0%
ATAGAAAGTTGGATCTACAT+TGG + Chr7:37742167-37742186 None:intergenic 30.0%
!! CCGTTTTTCTTTTTCTATTG+AGG + Chr7:37742308-37742327 None:intergenic 30.0%
AGCTGTCCATATACTGATAA+GGG + Chr7:37742258-37742277 None:intergenic 35.0%
TAGCTGTCCATATACTGATA+AGG + Chr7:37742259-37742278 None:intergenic 35.0%
AGATCACCTCTTTCATTCTG+GGG - Chr7:37742366-37742385 MsG0780037987.01.T01:CDS 40.0%
ATATGAATTCTCGAGAGGAG+AGG + Chr7:37742233-37742252 None:intergenic 40.0%
CCAACTTTCTATTCACTCGT+GGG - Chr7:37742175-37742194 MsG0780037987.01.T01:CDS 40.0%
GAGATCACCTCTTTCATTCT+GGG - Chr7:37742365-37742384 MsG0780037987.01.T01:CDS 40.0%
GGGATATATGAATTCTCGAG+AGG + Chr7:37742238-37742257 None:intergenic 40.0%
GTCTGTGAAGATACGTTGTT+AGG + Chr7:37742335-37742354 None:intergenic 40.0%
TCCAACTTTCTATTCACTCG+TGG - Chr7:37742174-37742193 MsG0780037987.01.T01:CDS 40.0%
AGAGGTGATCTCGTAGTTCT+TGG + Chr7:37742357-37742376 None:intergenic 45.0%
CCCACGAGTGAATAGAAAGT+TGG + Chr7:37742178-37742197 None:intergenic 45.0%
CGAGATCACCTCTTTCATTC+TGG - Chr7:37742364-37742383 MsG0780037987.01.T01:CDS 45.0%
TTCTATTCACTCGTGGGATC+CGG - Chr7:37742181-37742200 MsG0780037987.01.T01:CDS 45.0%
TTCTCCTCAGGAGGATAGAT+GGG - Chr7:37742125-37742144 MsG0780037987.01.T01:CDS 45.0%
AACCTAAACCTGTGCTCGAG+AGG + Chr7:37742282-37742301 None:intergenic 50.0%
CTTCTCCTCAGGAGGATAGA+TGG - Chr7:37742124-37742143 MsG0780037987.01.T01:CDS 50.0%
GAGGATAGATGGGGCGATTT+AGG - Chr7:37742135-37742154 MsG0780037987.01.T01:CDS 50.0%
TACCTCTCGAGCACAGGTTT+AGG - Chr7:37742277-37742296 MsG0780037987.01.T01:CDS 50.0%
TCTATTCACTCGTGGGATCC+GGG - Chr7:37742182-37742201 MsG0780037987.01.T01:CDS 50.0%
TCTCCTCAGGAGGATAGATG+GGG - Chr7:37742126-37742145 MsG0780037987.01.T01:CDS 50.0%
! CTCGAGCACAGGTTTAGGTT+TGG - Chr7:37742282-37742301 MsG0780037987.01.T01:CDS 50.0%
!! CCTCTTTCATTCTGGGGTGA+CGG - Chr7:37742372-37742391 MsG0780037987.01.T01:CDS 50.0%
CCGTCACCCCAGAATGAAAG+AGG + Chr7:37742375-37742394 None:intergenic 55.0%
!! CTTTCATTCTGGGGTGACGG+CGG - Chr7:37742375-37742394 MsG0780037987.01.T01:CDS 55.0%
!! TTTCATTCTGGGGTGACGGC+GGG - Chr7:37742376-37742395 MsG0780037987.01.T01:CDS 55.0%
ATTCACTCGTGGGATCCGGG+CGG - Chr7:37742185-37742204 MsG0780037987.01.T01:CDS 60.0%
CGAGAGGAGAGGAGCCATGA+TGG + Chr7:37742222-37742241 None:intergenic 60.0%
GACAGCTACCTCTCGAGCAC+AGG - Chr7:37742271-37742290 MsG0780037987.01.T01:CDS 60.0%
TCGCCCCATCTATCCTCCTG+AGG + Chr7:37742132-37742151 None:intergenic 60.0%
CGGTCCAGGGGGGACCATCA+TGG - Chr7:37742205-37742224 MsG0780037987.01.T01:CDS 70.0%
GGAGCCATGATGGTCCCCCC+TGG + Chr7:37742212-37742231 None:intergenic 70.0%
ATGGTCCCCCCTGGACCGCC+CGG + Chr7:37742203-37742222 None:intergenic 75.0%
CGTGGGATCCGGGCGGTCCA+GGG - Chr7:37742192-37742211 MsG0780037987.01.T01:CDS 75.0%
GTGGGATCCGGGCGGTCCAG+GGG - Chr7:37742193-37742212 MsG0780037987.01.T01:CDS 75.0%
TCGTGGGATCCGGGCGGTCC+AGG - Chr7:37742191-37742210 MsG0780037987.01.T01:CDS 75.0%
TGGGATCCGGGCGGTCCAGG+GGG - Chr7:37742194-37742213 MsG0780037987.01.T01:CDS 75.0%
! GGGATCCGGGCGGTCCAGGG+GGG - Chr7:37742195-37742214 MsG0780037987.01.T01:CDS 80.0%
Chromosome Type Strat End Strand Name
Chr7 gene 37742120 37742416 37742120 ID=MsG0780037987.01;Name=MsG0780037987.01
Chr7 mRNA 37742120 37742416 37742120 ID=MsG0780037987.01.T01;Parent=MsG0780037987.01;Name=MsG0780037987.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|98
Chr7 exon 37742120 37742416 37742120 ID=MsG0780037987.01.T01:exon:5671;Parent=MsG0780037987.01.T01
Chr7 CDS 37742120 37742416 37742120 ID=MsG0780037987.01.T01:cds;Parent=MsG0780037987.01.T01
Chr7 mRNA 37742238 37742312 37742238 ID=MsG0780037987.01.T02;Parent=MsG0780037987.01;Name=MsG0780037987.01.T02;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|24
Chr7 exon 37742238 37742312 37742238 ID=MsG0780037987.01.T02:exon:5672;Parent=MsG0780037987.01.T02
Chr7 CDS 37742238 37742312 37742238 ID=MsG0780037987.01.T02:cds;Parent=MsG0780037987.01.T02
Gene Sequence

>MsG0780037987.01.T02

ATGGCTCCTCTCCTCTCGAGAATTCATATATCCCTTATCAGTATATGGACAGCTACCTCTCGAGCACAGGTTTAG

>MsG0780037987.01.T01

CAAACTTCTCCTCAGGAGGATAGATGGGGCGATTTAGGTGAGATCCAATGTAGATCCAACTTTCTATTCACTCGTGGGATCCGGGCGGTCCAGGGGGGACCATCATGGCTCCTCTCCTCTCGAGAATTCATATATCCCTTATCAGTATATGGACAGCTACCTCTCGAGCACAGGTTTAGGTTTGGCCTCAATAGAAAAAGAAAAACGGAGCACCTAACAACGTATCTTCACAGACCAAGAACTACGAGATCACCTCTTTCATTCTGGGGTGACGGCGGGATCGTACCATTCTCTTGA

Protein sequence

>MsG0780037987.01.T02

MAPLLSRIHISLISIWTATSRAQV*

>MsG0780037987.01.T01

QTSPQEDRWGDLGEIQCRSNFLFTRGIRAVQGGPSWLLSSREFIYPLSVYGQLPLEHRFRFGLNRKRKTEHLTTYLHRPRTTRSPLSFWGDGGIVPFS*