AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280009867.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280009867.01.T01 MTR_2g072510 96.226 212 8 0 3 214 66 277 8.82e-148 412
MsG0280009867.01.T01 MTR_1g115890 29.126 206 137 5 17 214 13 217 8.87e-18 79.0
MsG0280009867.01.T01 MTR_3g093980 27.273 198 135 5 24 213 31 227 1.86e-16 75.5
MsG0280009867.01.T01 MTR_1g115870 29.126 206 137 5 17 214 13 217 2.93e-16 74.7
MsG0280009867.01.T01 MTR_1g115840 27.586 203 142 3 17 214 15 217 3.20e-16 74.7
MsG0280009867.01.T01 MTR_3g093980 28.070 171 117 4 48 213 6 175 8.22e-15 70.1
MsG0280009867.01.T01 MTR_3g093980 25.185 135 95 2 23 151 10 144 9.44e-13 63.9
MsG0280009867.01.T01 MTR_1g115850 24.752 202 147 3 18 214 16 217 1.22e-11 62.0
MsG0280009867.01.T01 MTR_1g115850 25.294 170 122 3 50 214 55 224 5.48e-11 60.5
MsG0280009867.01.T01 MTR_1g115820 25.248 202 146 3 17 213 12 213 5.87e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280009867.01.T01 AT1G53520 64.623 212 74 1 3 213 75 286 2.07e-96 283
MsG0280009867.01.T01 AT5G05270 26.316 190 133 5 30 213 20 208 5.19e-11 60.5
MsG0280009867.01.T01 AT5G05270 26.316 190 133 5 30 213 20 208 5.19e-11 60.5

Find 47 sgRNAs with CRISPR-Local

Find 99 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTCAAAGTTGTATATTAT+TGG 0.137358 2:-59568747 None:intergenic
ATGTTGATGGTAAAACTTTC+TGG 0.189488 2:+59568123 MsG0280009867.01.T01:CDS
AAATAAACTTACAAGCAATT+TGG 0.276458 2:-59568288 None:intergenic
ATGATATAAAATATTTGATT+TGG 0.282192 2:+59568724 MsG0280009867.01.T01:three_prime_UTR
GAAATTGTTACATTGGAAAA+TGG 0.299638 2:-59567799 None:intergenic
AAAATAAATAATAGCGTCTC+TGG 0.314816 2:+59568531 MsG0280009867.01.T01:exon
GGCGTCAAGGTCTATGCTTC+TGG 0.323231 2:+59567684 MsG0280009867.01.T01:CDS
TCAGACATCTTTGAGTTTCC+CGG 0.335505 2:+59566945 MsG0280009867.01.T01:CDS
TCAGATTCATTAACATTGTT+TGG 0.344207 2:+59566973 MsG0280009867.01.T01:CDS
TGAAATTTCACATTTGTTGC+TGG 0.346422 2:-59566925 None:intergenic
TGGTGATATGGCATCACTTA+AGG 0.366207 2:-59568150 None:intergenic
TTGAGCCACTGAAGCTTTCA+AGG 0.383492 2:-59568485 None:intergenic
CAGAAATAATACATACAAAT+AGG 0.389539 2:-59568779 None:intergenic
TCATTAACATTGTTTGGAAC+TGG 0.391138 2:+59566979 MsG0280009867.01.T01:CDS
CAGGTTTCTGTCTCGTCTGA+AGG 0.400923 2:+59568370 MsG0280009867.01.T01:intron
GCTTTCAAGGAGGGAGATAC+AGG 0.424288 2:-59568472 None:intergenic
GTTACTGCAGTTAAGCTCTA+TGG 0.439187 2:+59568604 MsG0280009867.01.T01:three_prime_UTR
GCAGTTTCATCTGCAGTTGT+TGG 0.451903 2:-59568185 None:intergenic
ACTCTTGTAAGAGATGTTGA+TGG 0.451968 2:+59568110 MsG0280009867.01.T01:CDS
ATTGTAGCATCCGCGGTAGA+TGG 0.462850 2:-59568397 None:intergenic
TAGAAAGAAACACTCTTGTT+CGG 0.471451 2:+59568803 MsG0280009867.01.T01:three_prime_UTR
TCAACTCATTTACGATAGAC+TGG 0.499366 2:-59567719 None:intergenic
CAGTCGAAAGATGTTATTCA+AGG 0.515053 2:+59567756 MsG0280009867.01.T01:CDS
GCCACTGAAGCTTTCAAGGA+GGG 0.518744 2:-59568481 None:intergenic
CAGACATCTTTGAGTTTCCC+GGG 0.519909 2:+59566946 MsG0280009867.01.T01:CDS
TGTTGATGGTAAAACTTTCT+GGG 0.525779 2:+59568124 MsG0280009867.01.T01:CDS
AGGTTTCTGTCTCGTCTGAA+GGG 0.528945 2:+59568371 MsG0280009867.01.T01:intron
AAACTTACAAGCAATTTGGA+AGG 0.544475 2:-59568284 None:intergenic
TCGTAAATGAGTTGAATGCT+TGG 0.553867 2:+59567727 MsG0280009867.01.T01:CDS
TAATTTGCAATAATTTCTCA+AGG 0.558300 2:-59568088 None:intergenic
ATTTGCAATAATTTCTCAAG+GGG 0.567136 2:-59568086 None:intergenic
TCCCTCCTTGAAAGCTTCAG+TGG 0.568299 2:+59568480 MsG0280009867.01.T01:CDS
GTTAATGAATCTGAGCAACC+CGG 0.573404 2:-59566964 None:intergenic
ATGAGTTGAATGCTTGGAAA+GGG 0.574681 2:+59567733 MsG0280009867.01.T01:CDS
AAATAAATAATAGCGTCTCT+GGG 0.577282 2:+59568532 MsG0280009867.01.T01:three_prime_UTR
TTGGAAAATGGTCTTGAACA+AGG 0.586433 2:-59567787 None:intergenic
AATGAGTTGAATGCTTGGAA+AGG 0.600633 2:+59567732 MsG0280009867.01.T01:CDS
GACGGTCTTGAAAGACGCTA+CGG 0.616631 2:-59568220 None:intergenic
TGCAAATGCTGAATACATTG+AGG 0.620913 2:+59566900 MsG0280009867.01.T01:CDS
TGAACGATTTGAAGTAAACA+AGG 0.622249 2:-59568569 None:intergenic
AATTTGCAATAATTTCTCAA+GGG 0.628018 2:-59568087 None:intergenic
AGTCGAAAGATGTTATTCAA+GGG 0.643960 2:+59567757 MsG0280009867.01.T01:CDS
AGATTCAATTGTAGCATCCG+CGG 0.657060 2:-59568404 None:intergenic
TTAATGAATCTGAGCAACCC+GGG 0.660829 2:-59566963 None:intergenic
AGCCACTGAAGCTTTCAAGG+AGG 0.667896 2:-59568482 None:intergenic
ATTATACATACAGCATACAA+CGG 0.704694 2:-59568631 None:intergenic
TGAAGGGATTCCATCTACCG+CGG 0.714392 2:+59568387 MsG0280009867.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGATATAAAATATTTGATT+TGG + Chr2:59568724-59568743 MsG0280009867.01.T01:three_prime_UTR 10.0%
!! TGTTTATAAAAAGAAATAAA+TGG - Chr2:59567829-59567848 None:intergenic 10.0%
!! AAATATCTAATTCTACAAAA+TGG - Chr2:59567424-59567443 None:intergenic 15.0%
!! TGAAAACATTATGTTTATAA+TGG + Chr2:59567498-59567517 MsG0280009867.01.T01:intron 15.0%
!!! AAATTAAAGTTCCTTTTTTA+AGG - Chr2:59568247-59568266 None:intergenic 15.0%
!!! AATTAAAGTTCCTTTTTTAA+GGG - Chr2:59568246-59568265 None:intergenic 15.0%
!! AAATAAACTTACAAGCAATT+TGG - Chr2:59568291-59568310 None:intergenic 20.0%
!! AATTTGCAATAATTTCTCAA+GGG - Chr2:59568090-59568109 None:intergenic 20.0%
!! CAGAAATAATACATACAAAT+AGG - Chr2:59568782-59568801 None:intergenic 20.0%
!! CATTCAAAGTTGTATATTAT+TGG - Chr2:59568750-59568769 None:intergenic 20.0%
!!! AAACTAAATTTTGTGATTTC+AGG + Chr2:59568351-59568370 MsG0280009867.01.T01:intron 20.0%
!!! ATTAAAGTTCCTTTTTTAAG+GGG - Chr2:59568245-59568264 None:intergenic 20.0%
!!! GAAAAAGTTTTTGCTATTAT+TGG + Chr2:59567663-59567682 MsG0280009867.01.T01:CDS 20.0%
!!! GAACTTTAATTTTTTTGACT+TGG + Chr2:59568255-59568274 MsG0280009867.01.T01:CDS 20.0%
!!! TAATTTGCAATAATTTCTCA+AGG - Chr2:59568091-59568110 None:intergenic 20.0%
!!! TTACATGACAACATTTTAAA+TGG + Chr2:59567049-59567068 MsG0280009867.01.T01:intron 20.0%
! AAAATAAATAATAGCGTCTC+TGG + Chr2:59568531-59568550 MsG0280009867.01.T01:exon 25.0%
! AAATAAATAATAGCGTCTCT+GGG + Chr2:59568532-59568551 MsG0280009867.01.T01:three_prime_UTR 25.0%
! ATTATACATACAGCATACAA+CGG - Chr2:59568634-59568653 None:intergenic 25.0%
! ATTTGCAATAATTTCTCAAG+GGG - Chr2:59568089-59568108 None:intergenic 25.0%
! GAAATTGTTACATTGGAAAA+TGG - Chr2:59567802-59567821 None:intergenic 25.0%
! GGAAAAAGAATGTTGAAAAA+TGG + Chr2:59567348-59567367 MsG0280009867.01.T01:intron 25.0%
! TATAATGTCGCAATTTAATG+TGG + Chr2:59567104-59567123 MsG0280009867.01.T01:intron 25.0%
! TCAGATTCATTAACATTGTT+TGG + Chr2:59566973-59566992 MsG0280009867.01.T01:CDS 25.0%
! TGGATATGAAATTGTTACAT+TGG - Chr2:59567809-59567828 None:intergenic 25.0%
!! AATGGATTCATACATATCTA+AGG + Chr2:59567366-59567385 MsG0280009867.01.T01:intron 25.0%
!! ATTCACAACAAGATTTTGAA+AGG - Chr2:59567248-59567267 None:intergenic 25.0%
!! TTGATAATAACACCTATTTC+AGG + Chr2:59567633-59567652 MsG0280009867.01.T01:intron 25.0%
AAACTTACAAGCAATTTGGA+AGG - Chr2:59568287-59568306 None:intergenic 30.0%
AATCAGTGTTGTCAAATAAC+CGG - Chr2:59568022-59568041 None:intergenic 30.0%
ACAACACTTTGCACTAAATT+TGG + Chr2:59567940-59567959 MsG0280009867.01.T01:intron 30.0%
ACCTACTAAATTCTACTAGA+CGG - Chr2:59567567-59567586 None:intergenic 30.0%
AGTCGAAAGATGTTATTCAA+GGG + Chr2:59567757-59567776 MsG0280009867.01.T01:CDS 30.0%
CTACTAGACGGAAATAATTA+CGG - Chr2:59567555-59567574 None:intergenic 30.0%
GAAAGATACAGCTTATTATC+AGG - Chr2:59567299-59567318 None:intergenic 30.0%
GGCAAAAATAGATGTATCAT+TGG - Chr2:59568872-59568891 MsG0280009868.01.T01:three_prime_UTR 30.0%
TACTAGACGGAAATAATTAC+GGG - Chr2:59567554-59567573 None:intergenic 30.0%
TAGAAAGAAACACTCTTGTT+CGG + Chr2:59568803-59568822 MsG0280009867.01.T01:three_prime_UTR 30.0%
TATCAATCAATCTAGAGACA+AGG - Chr2:59567173-59567192 None:intergenic 30.0%
TTGCACTAAATTTGGTATCA+CGG + Chr2:59567948-59567967 MsG0280009867.01.T01:intron 30.0%
! ATATCTAAGGTTAAACTTCG+AGG + Chr2:59567379-59567398 MsG0280009867.01.T01:intron 30.0%
! GCTCTTTTTGATGTATTTCT+TGG + Chr2:59568442-59568461 MsG0280009867.01.T01:CDS 30.0%
! TCATTAACATTGTTTGGAAC+TGG + Chr2:59566979-59566998 MsG0280009867.01.T01:CDS 30.0%
! TGAAATTTCACATTTGTTGC+TGG - Chr2:59566928-59566947 None:intergenic 30.0%
! TGAACGATTTGAAGTAAACA+AGG - Chr2:59568572-59568591 None:intergenic 30.0%
! TTTTCTCTGTATCCTGAAAT+AGG - Chr2:59567648-59567667 None:intergenic 30.0%
!! ATGTTGATGGTAAAACTTTC+TGG + Chr2:59568123-59568142 MsG0280009867.01.T01:CDS 30.0%
!! ATTTTCTAGTGATCGAAACT+TGG + Chr2:59568682-59568701 MsG0280009867.01.T01:three_prime_UTR 30.0%
!! TGTTGATGGTAAAACTTTCT+GGG + Chr2:59568124-59568143 MsG0280009867.01.T01:CDS 30.0%
!! TTTTGCAATTCTTGGTGATA+TGG - Chr2:59568165-59568184 None:intergenic 30.0%
ACAGGCAAAGTGATTCTATT+TGG + Chr2:59567326-59567345 MsG0280009867.01.T01:intron 35.0%
ACTCTTGTAAGAGATGTTGA+TGG + Chr2:59568110-59568129 MsG0280009867.01.T01:CDS 35.0%
CAGGCAAAGTGATTCTATTT+GGG + Chr2:59567327-59567346 MsG0280009867.01.T01:intron 35.0%
CAGTCGAAAGATGTTATTCA+AGG + Chr2:59567756-59567775 MsG0280009867.01.T01:CDS 35.0%
CTGTATCTTTCAGTAACTAC+AGG + Chr2:59567308-59567327 MsG0280009867.01.T01:intron 35.0%
GATCACGGAAAATAGAAGTT+TGG + Chr2:59567880-59567899 MsG0280009867.01.T01:intron 35.0%
GCAAGATAGCAAAAGTGAAA+AGG - Chr2:59568319-59568338 None:intergenic 35.0%
GGCAAAAAAATCCAAAGGTT+CGG - Chr2:59567533-59567552 None:intergenic 35.0%
GTGTTGTCAATAACTGATCA+CGG + Chr2:59567865-59567884 MsG0280009867.01.T01:intron 35.0%
TCAACTCATTTACGATAGAC+TGG - Chr2:59567722-59567741 None:intergenic 35.0%
TCCGTCTAGTAGAATTTAGT+AGG + Chr2:59567563-59567582 MsG0280009867.01.T01:intron 35.0%
TGACGATGAACTTACTTTCA+TGG + Chr2:59567193-59567212 MsG0280009867.01.T01:intron 35.0%
TGCAAATGCTGAATACATTG+AGG + Chr2:59566900-59566919 MsG0280009867.01.T01:CDS 35.0%
TGTTGTCAAATAACCGGTTA+TGG - Chr2:59568016-59568035 None:intergenic 35.0%
TTACGGGCAAAAAAATCCAA+AGG - Chr2:59567538-59567557 None:intergenic 35.0%
! AATGAGTTGAATGCTTGGAA+AGG + Chr2:59567732-59567751 MsG0280009867.01.T01:CDS 35.0%
! ATGAGTTGAATGCTTGGAAA+GGG + Chr2:59567733-59567752 MsG0280009867.01.T01:CDS 35.0%
! TCGTAAATGAGTTGAATGCT+TGG + Chr2:59567727-59567746 MsG0280009867.01.T01:CDS 35.0%
! TTGGAAAATGGTCTTGAACA+AGG - Chr2:59567790-59567809 None:intergenic 35.0%
!! TTTTGCTATCTTGCGAAAAC+TGG + Chr2:59568325-59568344 MsG0280009867.01.T01:intron 35.0%
!!! AAGTTCCTTTTTTAAGGGGA+CGG - Chr2:59568241-59568260 None:intergenic 35.0%
!!! GTTGTTGGTTTTGCAATTCT+TGG - Chr2:59568173-59568192 None:intergenic 35.0%
!!! TATGCTGAGATCACTGATTT+TGG + Chr2:59567128-59567147 MsG0280009867.01.T01:intron 35.0%
!!! TTTTGCTATTATTGGCGTCA+AGG + Chr2:59567671-59567690 MsG0280009867.01.T01:CDS 35.0%
AGATTCAATTGTAGCATCCG+CGG - Chr2:59568407-59568426 None:intergenic 40.0%
GTTAATGAATCTGAGCAACC+CGG - Chr2:59566967-59566986 None:intergenic 40.0%
GTTACTGCAGTTAAGCTCTA+TGG + Chr2:59568604-59568623 MsG0280009867.01.T01:three_prime_UTR 40.0%
TGGTGATATGGCATCACTTA+AGG - Chr2:59568153-59568172 None:intergenic 40.0%
TTAATGAATCTGAGCAACCC+GGG - Chr2:59566966-59566985 None:intergenic 40.0%
! TCAGACATCTTTGAGTTTCC+CGG + Chr2:59566945-59566964 MsG0280009867.01.T01:CDS 40.0%
AGGTTTCTGTCTCGTCTGAA+GGG + Chr2:59568371-59568390 MsG0280009867.01.T01:intron 45.0%
CAAGACCGTCCCCTTAAAAA+AGG + Chr2:59568233-59568252 MsG0280009867.01.T01:CDS 45.0%
GCAGTTTCATCTGCAGTTGT+TGG - Chr2:59568188-59568207 None:intergenic 45.0%
GGTATTGTATGCCGAACCTT+TGG + Chr2:59567519-59567538 MsG0280009867.01.T01:intron 45.0%
TATGGCAACGCTATAGCGTA+GGG - Chr2:59567998-59568017 None:intergenic 45.0%
TTATGGCAACGCTATAGCGT+AGG - Chr2:59567999-59568018 None:intergenic 45.0%
TTGAGCCACTGAAGCTTTCA+AGG - Chr2:59568488-59568507 None:intergenic 45.0%
! CAGACATCTTTGAGTTTCCC+GGG + Chr2:59566946-59566965 MsG0280009867.01.T01:CDS 45.0%
AGCCACTGAAGCTTTCAAGG+AGG - Chr2:59568485-59568504 None:intergenic 50.0%
ATGGCAACGCTATAGCGTAG+GGG - Chr2:59567997-59568016 None:intergenic 50.0%
ATTGTAGCATCCGCGGTAGA+TGG - Chr2:59568400-59568419 None:intergenic 50.0%
CAGGTTTCTGTCTCGTCTGA+AGG + Chr2:59568370-59568389 MsG0280009867.01.T01:intron 50.0%
CGCTATAGCGTTGCCATAAC+CGG + Chr2:59568000-59568019 MsG0280009867.01.T01:intron 50.0%
GACGGTCTTGAAAGACGCTA+CGG - Chr2:59568223-59568242 None:intergenic 50.0%
GCCACTGAAGCTTTCAAGGA+GGG - Chr2:59568484-59568503 None:intergenic 50.0%
GCTTTCAAGGAGGGAGATAC+AGG - Chr2:59568475-59568494 None:intergenic 50.0%
TCCCTCCTTGAAAGCTTCAG+TGG + Chr2:59568480-59568499 MsG0280009867.01.T01:CDS 50.0%
TGAAGGGATTCCATCTACCG+CGG + Chr2:59568387-59568406 MsG0280009867.01.T01:CDS 50.0%
GGCGTCAAGGTCTATGCTTC+TGG + Chr2:59567684-59567703 MsG0280009867.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr2 gene 59566886 59568896 59566886 ID=MsG0280009867.01;Name=MsG0280009867.01
Chr2 mRNA 59566886 59568896 59566886 ID=MsG0280009867.01.T01;Parent=MsG0280009867.01;Name=MsG0280009867.01.T01;_AED=0.40;_eAED=0.40;_QI=0|1|0.75|1|1|1|4|365|214
Chr2 exon 59566886 59567000 59566886 ID=MsG0280009867.01.T01:exon:6163;Parent=MsG0280009867.01.T01
Chr2 exon 59567655 59567810 59567655 ID=MsG0280009867.01.T01:exon:6164;Parent=MsG0280009867.01.T01
Chr2 exon 59568084 59568298 59568084 ID=MsG0280009867.01.T01:exon:6165;Parent=MsG0280009867.01.T01
Chr2 exon 59568373 59568896 59568373 ID=MsG0280009867.01.T01:exon:6166;Parent=MsG0280009867.01.T01
Chr2 CDS 59566886 59567000 59566886 ID=MsG0280009867.01.T01:cds;Parent=MsG0280009867.01.T01
Chr2 CDS 59567655 59567810 59567655 ID=MsG0280009867.01.T01:cds;Parent=MsG0280009867.01.T01
Chr2 CDS 59568084 59568298 59568084 ID=MsG0280009867.01.T01:cds;Parent=MsG0280009867.01.T01
Chr2 CDS 59568373 59568531 59568373 ID=MsG0280009867.01.T01:cds;Parent=MsG0280009867.01.T01
Chr2 three_prime_UTR 59568532 59568896 59568532 ID=MsG0280009867.01.T01:three_prime_utr;Parent=MsG0280009867.01.T01
Gene Sequence

>MsG0280009867.01.T01

TTGGATACTGCAGCTGCAAATGCTGAATACATTGAGGAACCAGCAACAAATGTGAAATTTCAGACATCTTTGAGTTTCCCGGGTTGCTCAGATTCATTAACATTGTTTGGAACTGGATACAGAGAAAAAGTTTTTGCTATTATTGGCGTCAAGGTCTATGCTTCTGGACTGTATCTAAACCAGTCTATCGTAAATGAGTTGAATGCTTGGAAAGGGCAGTCGAAAGATGTTATTCAAGGGAACTCTTCCTTGTTCAAGACCATTTTCCAATCTCCCCTTGAGAAATTATTGCAAATTACTCTTGTAAGAGATGTTGATGGTAAAACTTTCTGGGATGCCTTAAGTGATGCCATATCACCAAGAATTGCAAAACCAACAACTGCAGATGAAACTGCTTTGACTACTTTCCGTAGCGTCTTTCAAGACCGTCCCCTTAAAAAAGGAACTTTAATTTTTTTGACTTGGTTGAATCCTTCCAAATTGCTTGTTTCTGTCTCGTCTGAAGGGATTCCATCTACCGCGGATGCTACAATTGAATCTGCAAATGTGACTTGTGCTCTTTTTGATGTATTTCTTGGAGACAGTCCTGTATCTCCCTCCTTGAAAGCTTCAGTGGCTCAAAGCTTGTCAAAAGTACTCGAGTAA

Protein sequence

>MsG0280009867.01.T01

LDTAAANAEYIEEPATNVKFQTSLSFPGCSDSLTLFGTGYREKVFAIIGVKVYASGLYLNQSIVNELNAWKGQSKDVIQGNSSLFKTIFQSPLEKLLQITLVRDVDGKTFWDALSDAISPRIAKPTTADETALTTFRSVFQDRPLKKGTLIFLTWLNPSKLLVSVSSEGIPSTADATIESANVTCALFDVFLGDSPVSPSLKASVAQSLSKVLE*