Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1310700 | XP_024628503.1 | 97.217 | 575 | 13 | 1 | 5 | 579 | 3 | 574 | 0.0 | 1153 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1310700 | sp|Q8N9R8|SCAI_HUMAN | 34.336 | 565 | 319 | 13 | 21 | 561 | 62 | 598 | 8.83e-93 | 300 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1310700 | A0A072TME4 | 97.217 | 575 | 10 | 1 | 5 | 579 | 3 | 571 | 0.0 | 1152 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0002230 | Msa1310700 | 0.805116 | 1.558926e-49 | -8.615850e-47 |
| Msa0006110 | Msa1310700 | 0.813068 | 3.086660e-51 | -8.615850e-47 |
| Msa0009180 | Msa1310700 | 0.813404 | 2.604078e-51 | -8.615850e-47 |
| Msa0015390 | Msa1310700 | 0.812058 | 5.132739e-51 | -8.615850e-47 |
| Msa0021210 | Msa1310700 | 0.824529 | 7.684510e-54 | -8.615850e-47 |
| Msa0026910 | Msa1310700 | 0.835234 | 1.889319e-56 | -8.615850e-47 |
| Msa0031340 | Msa1310700 | 0.811751 | 5.984183e-51 | -8.615850e-47 |
| Msa0036140 | Msa1310700 | 0.804727 | 1.879720e-49 | -8.615850e-47 |
| Msa0037880 | Msa1310700 | 0.812260 | 4.637157e-51 | -8.615850e-47 |
| Msa0042920 | Msa1310700 | 0.800654 | 1.302518e-48 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1310700 | MtrunA17_Chr8g0337421 | 97.217 | 575 | 10 | 1 | 5 | 579 | 3 | 571 | 0.0 | 1152 |
| Msa1310700 | MtrunA17_Chr8g0381791 | 48.480 | 592 | 259 | 8 | 19 | 566 | 10 | 599 | 0.0 | 547 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1310700 | AT3G03570.1 | 58.007 | 612 | 195 | 7 | 7 | 568 | 3 | 602 | 0.0 | 711 |
| Msa1310700 | AT4G40050.1 | 46.000 | 600 | 264 | 8 | 17 | 564 | 6 | 597 | 0.0 | 525 |
| Msa1310700 | AT4G40050.2 | 46.370 | 427 | 179 | 5 | 17 | 394 | 6 | 431 | 3.10e-129 | 386 |
Find 143 sgRNAs with CRISPR-Local
Find 546 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAACCAAACTATACCTCAC+CGG | 0.626934 | 8_4:-7328373 | None:intergenic |
| AAAACCCAAACTTGTAAATG+TGG | 0.570497 | 8_4:-7335528 | None:intergenic |
| AAATAACTGGGGATTGGCGT+TGG | 0.503199 | 8_4:+7333953 | Msa1310700:CDS |
| AAATCGGAGAAATTGCGTCG+CGG | 0.756221 | 8_4:+7328005 | Msa1310700:CDS |
| AACCTGGAGCGTTGGTAATA+AGG | 0.311040 | 8_4:-7327800 | None:intergenic |
| AAGGTTGTTGTGGTGGAAAT+TGG | 0.304615 | 8_4:+7328310 | Msa1310700:CDS |
| AAGTGAAATTATCCTCTACC+GGG | 0.665603 | 8_4:+7327562 | Msa1310700:five_prime_UTR |
| AATAATGCCATTTCCAAAGA+AGG | 0.444819 | 8_4:-7335629 | None:intergenic |
| AATACCACATTTACAAGTTT+GGG | 0.304767 | 8_4:+7335524 | Msa1310700:three_prime_UTR |
| AATGCAGGAGCAGGATCTTC+TGG | 0.343711 | 8_4:+7330569 | Msa1310700:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TCAAAGTCTACACACAATTA+TGG | + | chr8_4:7327927-7327946 | Msa1310700:CDS | 30.0% | |
| CATTGTCTACCTCATTATAA+TGG | - | chr8_4:7328486-7328505 | None:intergenic | 30.0% | |
| CCTCTTTGATTGTAGAAATA+AGG | + | chr8_4:7328713-7328732 | Msa1310700:intron | 30.0% | |
| TTACTGTCTGTAACATGTTA+TGG | + | chr8_4:7329086-7329105 | Msa1310700:intron | 30.0% | |
| GTTCAAAACTTAGTCAGAAT+GGG | + | chr8_4:7329246-7329265 | Msa1310700:CDS | 30.0% | |
| TTCAAAACTTAGTCAGAATG+GGG | + | chr8_4:7329247-7329266 | Msa1310700:CDS | 30.0% | |
| ACATTTCATAGCTGTAAGTA+TGG | + | chr8_4:7329367-7329386 | Msa1310700:intron | 30.0% | |
| GATTTGATACAAGAACTACT+GGG | - | chr8_4:7329428-7329447 | None:intergenic | 30.0% | |
| TGATTTGATACAAGAACTAC+TGG | - | chr8_4:7329429-7329448 | None:intergenic | 30.0% | |
| GCCTATTATACTAGTAATCT+AGG | + | chr8_4:7329570-7329589 | Msa1310700:intron | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_4 | gene | 7327497 | 7335670 | 7327497 | ID=Msa1310700;Name=Msa1310700 |
| chr8_4 | mRNA | 7327497 | 7335670 | 7327497 | ID=Msa1310700-mRNA-1;Parent=Msa1310700;Name=Msa1310700-mRNA-1;_AED=0.02;_eAED=0.02;_QI=189|0.87|0.88|1|0.75|0.88|9|614|579 |
| chr8_4 | exon | 7327497 | 7327819 | 7327497 | ID=Msa1310700-mRNA-1:exon:2599;Parent=Msa1310700-mRNA-1 |
| chr8_4 | exon | 7327901 | 7328495 | 7327901 | ID=Msa1310700-mRNA-1:exon:2600;Parent=Msa1310700-mRNA-1 |
| chr8_4 | exon | 7329158 | 7329379 | 7329158 | ID=Msa1310700-mRNA-1:exon:2601;Parent=Msa1310700-mRNA-1 |
| chr8_4 | exon | 7330390 | 7330456 | 7330390 | ID=Msa1310700-mRNA-1:exon:2602;Parent=Msa1310700-mRNA-1 |
| chr8_4 | exon | 7330576 | 7330644 | 7330576 | ID=Msa1310700-mRNA-1:exon:2603;Parent=Msa1310700-mRNA-1 |
| chr8_4 | exon | 7332903 | 7333012 | 7332903 | ID=Msa1310700-mRNA-1:exon:2604;Parent=Msa1310700-mRNA-1 |
| chr8_4 | exon | 7333616 | 7333795 | 7333616 | ID=Msa1310700-mRNA-1:exon:2605;Parent=Msa1310700-mRNA-1 |
| chr8_4 | exon | 7333912 | 7334048 | 7333912 | ID=Msa1310700-mRNA-1:exon:2606;Parent=Msa1310700-mRNA-1 |
| chr8_4 | exon | 7334831 | 7335670 | 7334831 | ID=Msa1310700-mRNA-1:exon:2607;Parent=Msa1310700-mRNA-1 |
| chr8_4 | five_prime_UTR | 7327497 | 7327685 | 7327497 | ID=Msa1310700-mRNA-1:five_prime_utr;Parent=Msa1310700-mRNA-1 |
| chr8_4 | CDS | 7327686 | 7327819 | 7327686 | ID=Msa1310700-mRNA-1:cds;Parent=Msa1310700-mRNA-1 |
| chr8_4 | CDS | 7327901 | 7328495 | 7327901 | ID=Msa1310700-mRNA-1:cds;Parent=Msa1310700-mRNA-1 |
| chr8_4 | CDS | 7329158 | 7329379 | 7329158 | ID=Msa1310700-mRNA-1:cds;Parent=Msa1310700-mRNA-1 |
| chr8_4 | CDS | 7330390 | 7330456 | 7330390 | ID=Msa1310700-mRNA-1:cds;Parent=Msa1310700-mRNA-1 |
| chr8_4 | CDS | 7330576 | 7330644 | 7330576 | ID=Msa1310700-mRNA-1:cds;Parent=Msa1310700-mRNA-1 |
| chr8_4 | CDS | 7332903 | 7333012 | 7332903 | ID=Msa1310700-mRNA-1:cds;Parent=Msa1310700-mRNA-1 |
| chr8_4 | CDS | 7333616 | 7333795 | 7333616 | ID=Msa1310700-mRNA-1:cds;Parent=Msa1310700-mRNA-1 |
| chr8_4 | CDS | 7333912 | 7334048 | 7333912 | ID=Msa1310700-mRNA-1:cds;Parent=Msa1310700-mRNA-1 |
| chr8_4 | CDS | 7334831 | 7335056 | 7334831 | ID=Msa1310700-mRNA-1:cds;Parent=Msa1310700-mRNA-1 |
| chr8_4 | three_prime_UTR | 7335057 | 7335670 | 7335057 | ID=Msa1310700-mRNA-1:three_prime_utr;Parent=Msa1310700-mRNA-1 |
| Gene Sequence |
>Msa1310700 ATGGCGCAACAGCAGCAACAACAACAACAGCTGAACAGCAACATCCCAGTAAGCGAAGTTTTCTGGACTCTCGTCGACAAAGCCGACAAAAAATTCTCCAAAATCAGAGACTTACCTTATTACCAACGCTCCAGGCATGATACTTATTTCTGCAAGGTTTTCAAAGTCTACACACAATTATGGAAATTCCAACAAGAAAATCGCCAGAAACTAATCGAAGCAGGGTTGAAAAGATGGGAAATCGGAGAAATTGCGTCGCGGATTGGTCAATTGTATTTTGGACAATATATGAAAACTTCTGATTACAATTATTTATCAGAATCTTACATTTTCTATGAAGCGATATTCACGCGCGATTATTTCAGAGAGGGATTGTTTTCGGATGTTAATATTGCGAATAAGCAGTTGAGGTTTTTGGCGAGGTTTTTAACGGTGTGTTTGCTTTTGAATCGGAGGGAAATGTTGCAACAATTGGTTAATCAGCTGAAAGTGTTGGTTGATGAGTGTAAGAGAGTGTTTGTGGATAGTGATTTTAAGGAGTGGAAGGTTGTTGTGGTGGAAATTGGGAGGTTTTTGAAGGTTGATACGGGTTTTATGAATGTTAGACCGGTGAGGTATAGTTTGGTTTTGGATTCTCATCCTGATACTTTACCGCGTGTTCCTGTTGCTATCGCGAAGCGGAATTTGAAATTAAGAGATGCTGTGTTGAGTAGCTTCCATTATAATGAGGTTAAGTTTTCAGAGTTCACTATTGACACTTTTAGGATGCTTCAATGTTTAGAGTGGGAACCTAGTGGCTCATTTTACCAGTCTGGTGGTTCAAAACTTAGTCAGAATGGGGCAACTGGGACTGGTCGTATCAGTTACATACAAGATATTGCTGATCCTACGTTACCAGCAAACCCACGGAAAGCTATATTATACCGTCCTTCACTGACACATTTCATAGCTGTTCTTGCCACAATTTGTGAGGAGCTTCCTTTGGATGGTATTCTCCTTGTATATTTGTCAGCTTCAGGAGCAGGATCTTCTGGTGCCGGGAATAATGAATCTGGTTGCATAAACTTAGGTTCCCGTGGAGATAGAGGATCAAACTCCATATATCCATCGGACTTTCTACCGTTTACTAGAAGACCACTTCTTTTAATCATTGATAATGAGAACAGCAAAGCTTTTAAGGCAAGCCCTACTACTTCAGTCATAACTGAAGCAGAAAAAGGAGAGTCAGTGGCAATGCTTCTTTCTCCAAGCCGTTTGCCTCCTTTTGTGTCTGATTGCTCACATTCTTCAAATGGGAGCCTATTCACCATGTTTCTTACAGCTCCGCTACAAGCTTTCTGTTTGCTGCTAGGATTTTCAGGCACTGATATTGATTTGGACTTGTACAACAAAGCTGAAACACTGCTGTCATCATCATTAAATAACTGGGGATTGGCGTTGGCATCAACAGACACGCTTGATCCAGTTTGGGGACAGGTTTTAGGCGATCCCTTCATAAGGAGACTTCTTATGAGATTCATATTTTGCCAGACAGTGTTGACCTCGTATGCACCAGTTTATGATAAAAATGAATTCCTTCCAACATGTGTCCCTTCTCTTCCAACGCCTGTCCTAGCTCCATGCTGCTCGTATCAAAGTGTAATTCTGCAACTAGCTGGCATTTTTGGTGCAACTAAGCACTTCATCTTTTCTGAGGACGTTTCTCCTAAAAATACACTTCCAAACGTGGATCAGTTGTGA |
| Protein sequence |
>Msa1310700 MAQQQQQQQQLNSNIPVSEVFWTLVDKADKKFSKIRDLPYYQRSRHDTYFCKVFKVYTQLWKFQQENRQKLIEAGLKRWEIGEIASRIGQLYFGQYMKTSDYNYLSESYIFYEAIFTRDYFREGLFSDVNIANKQLRFLARFLTVCLLLNRREMLQQLVNQLKVLVDECKRVFVDSDFKEWKVVVVEIGRFLKVDTGFMNVRPVRYSLVLDSHPDTLPRVPVAIAKRNLKLRDAVLSSFHYNEVKFSEFTIDTFRMLQCLEWEPSGSFYQSGGSKLSQNGATGTGRISYIQDIADPTLPANPRKAILYRPSLTHFIAVLATICEELPLDGILLVYLSASGAGSSGAGNNESGCINLGSRGDRGSNSIYPSDFLPFTRRPLLLIIDNENSKAFKASPTTSVITEAEKGESVAMLLSPSRLPPFVSDCSHSSNGSLFTMFLTAPLQAFCLLLGFSGTDIDLDLYNKAETLLSSSLNNWGLALASTDTLDPVWGQVLGDPFIRRLLMRFIFCQTVLTSYAPVYDKNEFLPTCVPSLPTPVLAPCCSYQSVILQLAGIFGATKHFIFSEDVSPKNTLPNVDQL* |
