AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0463850


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0463850 MtrunA17_Chr3g0114271 98.592 71 1 0 1 71 1 71 9.64e-46 140
Msa0463850 MtrunA17_Chr5g0437891 85.507 69 10 0 1 69 1 69 6.75e-28 95.5
Msa0463850 MtrunA17_Chr1g0198341 32.857 70 47 0 2 71 3 72 2.34e-11 53.9
Msa0463850 MtrunA17_Chr4g0067291 44.828 58 32 0 7 64 78 135 2.96e-11 57.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0463850 AT5G05365.1 73.529 68 18 0 1 68 1 68 1.94e-30 102

Find 12 sgRNAs with CRISPR-Local

Find 25 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTAAACTAAAATTTCAATTA+TGG 0.153724 3_3:+63406978 None:intergenic
ATTAGAGTCCTTCACAAAAT+TGG 0.192675 3_3:-63407037 Msa0463850:CDS
TAAACTAAAATTTCAATTAT+GGG 0.274782 3_3:+63406979 None:intergenic
TTATATAATATATATGTTCA+TGG 0.362890 3_3:-63406898 Msa0463850:three_prime_UTR
CTGAACAAGGTTATTGTTAC+GGG 0.372260 3_3:-63407082 Msa0463850:CDS
GCTGAACAAGGTTATTGTTA+CGG 0.424660 3_3:-63407083 Msa0463850:CDS
GGTTTAAGCAGCATCTTCAA+AGG 0.529778 3_3:+63407000 None:intergenic
GTTTAAGCAGCATCTTCAAA+GGG 0.559014 3_3:+63407001 None:intergenic
CGTGGACACAAAGCTGAACA+AGG 0.593500 3_3:-63407095 Msa0463850:CDS
CAGGTGGTGGAAGTCAAAGT+CGG 0.656628 3_3:-63407328 Msa0463850:CDS
TTGTATCAAGAAAATCCTCA+AGG 0.661316 3_3:-63407290 Msa0463850:CDS
AGATATTGAAACGTATAACG+TGG 0.667545 3_3:-63407113 Msa0463850:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAACTAAAATTTCAATTAT+GGG + chr3_3:63407350-63407369 None:intergenic 10.0%
!! TTATATAATATATATGTTCA+TGG - chr3_3:63407428-63407447 Msa0463850:intron 10.0%
!! ATATTATATAAGAACAACTA+AGG + chr3_3:63407180-63407199 None:intergenic 15.0%
!! CTAAACTAAAATTTCAATTA+TGG + chr3_3:63407351-63407370 None:intergenic 15.0%
!! TAGAAATTTTACAATCAGCA+TGG - chr3_3:63406881-63406900 Msa0463850:three_prime_UTR 25.0%
! AACAATAGAAAGATGTTCAA+TGG + chr3_3:63406923-63406942 None:intergenic 25.0%
!!! AGAACCTTGAATTTTTTTGA+TGG + chr3_3:63407062-63407081 None:intergenic 25.0%
!!! GTAGCTATGTTAGATTTTTT+GGG + chr3_3:63407099-63407118 None:intergenic 25.0%
!!! AGTAGCTATGTTAGATTTTT+TGG + chr3_3:63407100-63407119 None:intergenic 25.0%
!!! TGTTTTTGAGAAAATTAAGC+AGG + chr3_3:63407399-63407418 None:intergenic 25.0%
TTGTATCAAGAAAATCCTCA+AGG - chr3_3:63407036-63407055 Msa0463850:CDS 30.0%
AAGGCCATCAAAAAAATTCA+AGG - chr3_3:63407055-63407074 Msa0463850:CDS 30.0%
AGATATTGAAACGTATAACG+TGG - chr3_3:63407213-63407232 Msa0463850:intron 30.0%
ATTAGAGTCCTTCACAAAAT+TGG - chr3_3:63407289-63407308 Msa0463850:CDS 30.0%
! CATTCTTTCCAATTTTGTGA+AGG + chr3_3:63407300-63407319 None:intergenic 30.0%
TCTTCTTATGTGTCATCATC+AGG - chr3_3:63406979-63406998 Msa0463850:three_prime_UTR 35.0%
!!! GAATTTTTTTGATGGCCTTG+AGG + chr3_3:63407054-63407073 None:intergenic 35.0%
GCTGAACAAGGTTATTGTTA+CGG - chr3_3:63407243-63407262 Msa0463850:intron 35.0%
CTGAACAAGGTTATTGTTAC+GGG - chr3_3:63407244-63407263 Msa0463850:intron 35.0%
GTTTAAGCAGCATCTTCAAA+GGG + chr3_3:63407328-63407347 None:intergenic 35.0%
TCTTATGTGTCATCATCAGG+TGG - chr3_3:63406982-63407001 Msa0463850:three_prime_UTR 40.0%
GGTTTAAGCAGCATCTTCAA+AGG + chr3_3:63407329-63407348 None:intergenic 40.0%
TATGTGTCATCATCAGGTGG+TGG - chr3_3:63406985-63407004 Msa0463850:exon 45.0%
!! CAGGTGGTGGAAGTCAAAGT+CGG - chr3_3:63406998-63407017 Msa0463850:exon 50.0%
CGTGGACACAAAGCTGAACA+AGG - chr3_3:63407231-63407250 Msa0463850:intron 50.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 63406860 63407488 63406860 ID=Msa0463850;Name=Msa0463850
chr3_3 mRNA 63406860 63407488 63406860 ID=Msa0463850-mRNA-1;Parent=Msa0463850;Name=Msa0463850-mRNA-1;_AED=0.43;_eAED=0.43;_QI=40|1|1|1|1|1|3|143|71
chr3_3 exon 63406860 63407133 63406860 ID=Msa0463850-mRNA-1:exon:13831;Parent=Msa0463850-mRNA-1
chr3_3 exon 63407272 63407347 63407272 ID=Msa0463850-mRNA-1:exon:13830;Parent=Msa0463850-mRNA-1
chr3_3 exon 63407440 63407488 63407440 ID=Msa0463850-mRNA-1:exon:13829;Parent=Msa0463850-mRNA-1
chr3_3 five_prime_UTR 63407449 63407488 63407449 ID=Msa0463850-mRNA-1:five_prime_utr;Parent=Msa0463850-mRNA-1
chr3_3 CDS 63407440 63407448 63407440 ID=Msa0463850-mRNA-1:cds;Parent=Msa0463850-mRNA-1
chr3_3 CDS 63407272 63407347 63407272 ID=Msa0463850-mRNA-1:cds;Parent=Msa0463850-mRNA-1
chr3_3 CDS 63407003 63407133 63407003 ID=Msa0463850-mRNA-1:cds;Parent=Msa0463850-mRNA-1
chr3_3 three_prime_UTR 63406860 63407002 63406860 ID=Msa0463850-mRNA-1:three_prime_utr;Parent=Msa0463850-mRNA-1
Gene Sequence

>Msa0463850

ATGGCTAATGTGGTGGAAGTCAAAGTCGGTTTGCACTGTGACGATTGTATCAAGAAAATCCTCAAGGCCATCAAAAAAATTCAAGATATTGAAACGTATAACGTGGACACAAAGCTGAACAAGGTTATTGTTACGGGTAACGTAACAACAGAACAAGTTATTAGAGTCCTTCACAAAATTGGAAAGAATGCAACTCCCTTTGAAGATGCTGCTTAA

Protein sequence

>Msa0463850

MANVVEVKVGLHCDDCIKKILKAIKKIQDIETYNVDTKLNKVIVTGNVTTEQVIRVLHKIGKNATPFEDAA*