AlfalfaGEDB | Msa0144510

M. sativa cultivar zhongmu-4 / Msa0144510

KEGG: K15397
GO: GO:0003674 GO:0003824 GO:0004312 GO:0006725 GO:0006950 GO:0006970 GO:0008150 GO:0008152 GO:0009058 GO:0009266 GO:0009314 GO:0009409 GO:0009416 GO:0009611 GO:0009628 GO:0009698 GO:0009699 GO:0009922 GO:0009987 GO:0010345 GO:0016740 GO:0016746 GO:0016747 GO:0019438 GO:0019748 GO:0044237 GO:0044249 GO:0044550 GO:0050896 GO:0071704 GO:1901360 GO:1901362 GO:1901576
PFAM: PF08392.15

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0144510	sp\|Q5XEP9\|KCS2_ARATH	75.285	526	120	6	4	521	1	524	0.0	825

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0144510	G7ZZP2	98.273	521	9	0	1	521	1	521	0.0	1070

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000220	Msa0144510	0.870198	1.749180e-66	-8.615850e-47
Msa0001460	Msa0144510	0.842307	2.801608e-58	-8.615850e-47
Msa0001540	Msa0144510	0.822288	2.566367e-53	-8.615850e-47
Msa0003370	Msa0144510	0.804669	1.933622e-49	-8.615850e-47
Msa0006580	Msa0144510	0.844560	7.011288e-59	-8.615850e-47
Msa0009210	Msa0144510	0.816460	5.467877e-52	-8.615850e-47
Msa0012460	Msa0144510	0.809229	2.097885e-50	-8.615850e-47
Msa0015370	Msa0144510	0.940793	1.268370e-100	-8.615850e-47
Msa0017060	Msa0144510	0.820377	7.083082e-53	-8.615850e-47
Msa0017980	Msa0144510	0.830866	2.306558e-55	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	bit score
Msa0144510	MtrunA17_Chr1g0166191	98.273	521	9	0	1	521	1	521	1070
Msa0144510	MtrunA17_Chr1g0188051	85.631	515	68	3	11	521	2	514	933
Msa0144510	MtrunA17_Chr4g0063011	76.065	493	118	0	26	518	8	500	800
Msa0144510	MtrunA17_Chr4g0035081	66.469	507	161	2	6	512	5	502	707
Msa0144510	MtrunA17_Chr7g0276251	66.200	500	160	5	23	513	30	529	672
Msa0144510	MtrunA17_Chr2g0306781	84.759	374	57	0	145	518	32	405	660
Msa0144510	MtrunA17_Chr1g0209881	63.083	493	181	1	26	518	5	496	640
Msa0144510	MtrunA17_Chr5g0423521	60.000	495	197	1	24	518	3	496	618
Msa0144510	MtrunA17_Chr3g0136561	58.190	464	183	3	58	510	438	901	583
Msa0144510	MtrunA17_Chr6g0480561	62.125	433	163	1	83	514	38	470	576

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	bit score
Msa0144510	AT1G04220.1	75.285	526	120	6	4	521	1	524	825
Msa0144510	AT2G26640.1	76.065	493	118	0	26	518	17	509	810
Msa0144510	AT5G43760.1	76.613	496	110	3	32	521	34	529	773
Msa0144510	AT1G19440.1	68.454	485	152	1	26	510	29	512	698
Msa0144510	AT2G16280.1	65.385	494	170	1	17	510	16	508	695
Msa0144510	AT1G01120.1	62.097	496	182	3	23	512	31	526	665
Msa0144510	AT1G68530.1	60.484	496	195	1	23	518	3	497	642
Msa0144510	AT4G34510.1	63.312	477	168	3	37	512	15	485	639
Msa0144510	AT1G25450.1	60.243	493	195	1	26	518	1	492	631
Msa0144510	AT2G26250.1	56.008	516	196	5	23	510	36	548	595

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Find 109 sgRNAs with CRISPR-Local

Find 218 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACAAACATCACAACATT+AGG	0.368559	1_4:+4099741	Msa0144510:CDS
AAAAGAGGAAAGAAAGCAAA+GGG	0.495861	1_4:-4097123	None:intergenic
AAAAGTTTAAGGTAAAGAAG+TGG	0.543392	1_4:-4097153	None:intergenic
AAAATCTTGATTTAAGTGAT+TGG	0.369920	1_4:+4099928	Msa0144510:CDS
AAACTAAAATATGTGAAACT+TGG	0.549788	1_4:+4097393	Msa0144510:CDS
AAAGACCTAATGGCTGTTGC+CGG	0.418979	1_4:+4099708	Msa0144510:CDS
AAGAAAATACTTGAGAGGTC+AGG	0.497485	1_4:+4097738	Msa0144510:CDS
AAGCAACTTTGAAGATTAAT+TGG	0.219548	1_4:-4100444	None:intergenic
AATAAGAGCTGTTCCGACAT+AGG	0.515411	1_4:-4099777	None:intergenic
AATATCTTGAGTTATAATCT+TGG	0.479554	1_4:+4097993	Msa0144510:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAGAGGAAAGAAAGCAAA+GGG	-	chr1_4:4097126-4097145	None:intergenic	30.0%
ATCTTGAGTTATAATCTTGG+TGG	+	chr1_4:4097996-4098015	Msa0144510:CDS	30.0%
AAATGTGATTGGATTGAACT+AGG	-	chr1_4:4098151-4098170	None:intergenic	30.0%
ATCTGATCAACAAATGTGAT+TGG	-	chr1_4:4098162-4098181	None:intergenic	30.0%
TTCTCTGCATAACAATAACT+AGG	+	chr1_4:4098322-4098341	Msa0144510:intron	30.0%
AATTCAAGTCCATTTCTTCT+TGG	+	chr1_4:4098411-4098430	Msa0144510:intron	30.0%
TACAACCTGTACATTTGAAT+TGG	-	chr1_4:4098713-4098732	None:intergenic	30.0%
TTCAAATGTACAGGTTGTAA+AGG	+	chr1_4:4098714-4098733	Msa0144510:intron	30.0%
CAACATGTCAACATAAACAA+GGG	-	chr1_4:4098823-4098842	None:intergenic	30.0%
ACAACATGTCAACATAAACA+AGG	-	chr1_4:4098824-4098843	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_4	gene	4097078	4100459	4097078	ID=Msa0144510;Name=Msa0144510
chr1_4	mRNA	4097078	4100459	4097078	ID=Msa0144510-mRNA-1;Parent=Msa0144510;Name=Msa0144510-mRNA-1;_AED=0.02;_eAED=0.02;_QI=216\|1\|1\|1\|1\|1\|2\|236\|521
chr1_4	exon	4097078	4098073	4097078	ID=Msa0144510-mRNA-1:exon:1683;Parent=Msa0144510-mRNA-1
chr1_4	exon	4099438	4100459	4099438	ID=Msa0144510-mRNA-1:exon:1684;Parent=Msa0144510-mRNA-1
chr1_4	five_prime_UTR	4097078	4097293	4097078	ID=Msa0144510-mRNA-1:five_prime_utr;Parent=Msa0144510-mRNA-1
chr1_4	CDS	4097294	4098073	4097294	ID=Msa0144510-mRNA-1:cds;Parent=Msa0144510-mRNA-1
chr1_4	CDS	4099438	4100223	4099438	ID=Msa0144510-mRNA-1:cds;Parent=Msa0144510-mRNA-1
chr1_4	three_prime_UTR	4100224	4100459	4100224	ID=Msa0144510-mRNA-1:three_prime_utr;Parent=Msa0144510-mRNA-1

Gene Sequence

>Msa0144510

ATGCCCTTCATGGAACAGCAAAATAATCAACAAGATCATAATTCTAGAAGAAGCATTTCTTTTGAAAGAAGTAAACTCCCCAATTTTTTAATATCCGTGAAACTAAAATATGTGAAACTTGGTTACCACTATTTGATTTCTAATGCTATGTACCTTGTTTTAATACCTCTCTTTGGAGTAGCTTCAGTTCACTTATCAACTTTTTCAATCAAAGATTTAATCCAATTATATGAAAATCTCAAATTCAACTTTGTGTCAATGCTTATATGTTCAAGTCTAGTGGTATTTCTTGCAACCCTTTATTTCATGTCTCGTCCAAGAGGCATATACTTGGTTGATTTTGCATGCTACAAACCACAAAAAGAATTGCAAGTCACAAGAGAGATTTTCGTAGAAAGATCAATTTTAACAAAAGCATTCACAGAAGAAACTCTTTCTTTTCAAAAGAAAATACTTGAGAGGTCAGGTTTAGGGCAAAAGACATATTTGCCACCAGCAATAATGAGGGTCCCACCAAATCCTTGTATGGCTGAAGCAAGGAAAGAAGCTGAAGAGGTTATGTTTGGTGCTATTGATGAGGTTCTTCAGAAAACTGGTGTGAAGGCTAAGGATATTGGGATTTTGGTTGTGAATTGTAGTTTGTTTAATCCAACACCATCTCTTTCTGCCATGATTGTGAATCATTATAAATTGAGAGGAAATATCTTGAGTTATAATCTTGGTGGTATGGGTTGTAGTGCTGGTCTTATCTCTATTGACCTTGCCAAACAGCTCCTACAGGTACATCCAAACTCATATGCCTTAGTAGTGAGCATGGAGAACATAACACTAAACTGGTATTTCGGCAACGACAGATCAATGCTAGTGCCAAATTGTCTCTTTAGAATGGGAGGAGCAGCAGTTCTCCTTTCCAACAAGCCCCGCGACCGCCTCCGATCAAAATACCAACTAGTCCACACCGTCCGAACGCACAAAGGTGCAGACGACAAATCCTATGGCTGCGTCTTTCAAGAAGAAGACGACACAAAGCAAGTCGGTGTATCACTCTCAAAAGACCTAATGGCTGTTGCCGGAGAAGCACTCAAAACAAACATCACAACATTAGGACCATTAGTCCTACCTATGTCGGAACAGCTCTTATTTTTCGGGACATTGGTTGCGCGAAAAGTCTTCAAGATGAAAATCAAACCATACATTCCTGATTTTAAATTAGCTTTTGAGCATTTTTGTATTCATGCTGGTGGAAGAGCTGTTTTGGATGAGTTGGAGAAAAATCTTGATTTAAGTGATTGGCATATGGAACCATCAAGGATGACACTGAATAGATTTGGCAACACTTCAAGTAGTTCTTTGTGGTATGAATTGGCTTATACTGAAGCTAAAGGGAGGATTAAAAAAGGTGATAGAACTTGGCAAATTGCATTTGGTTCTGGTTTTAAGTGTAATAGTGCTGTTTGGAAAGCATTGAAGACTATTAATCCTGCAAAGGAGAAGAATCCTTGGATTGATGAGATTCATGAGTTTCCTGTTCATGTGCCTAAAGTGTCAAAAGTTATTGGTTCTTAA

Protein sequence

>Msa0144510

MPFMEQQNNQQDHNSRRSISFERSKLPNFLISVKLKYVKLGYHYLISNAMYLVLIPLFGVASVHLSTFSIKDLIQLYENLKFNFVSMLICSSLVVFLATLYFMSRPRGIYLVDFACYKPQKELQVTREIFVERSILTKAFTEETLSFQKKILERSGLGQKTYLPPAIMRVPPNPCMAEARKEAEEVMFGAIDEVLQKTGVKAKDIGILVVNCSLFNPTPSLSAMIVNHYKLRGNILSYNLGGMGCSAGLISIDLAKQLLQVHPNSYALVVSMENITLNWYFGNDRSMLVPNCLFRMGGAAVLLSNKPRDRLRSKYQLVHTVRTHKGADDKSYGCVFQEEDDTKQVGVSLSKDLMAVAGEALKTNITTLGPLVLPMSEQLLFFGTLVARKVFKMKIKPYIPDFKLAFEHFCIHAGGRAVLDELEKNLDLSDWHMEPSRMTLNRFGNTSSSSLWYELAYTEAKGRIKKGDRTWQIAFGSGFKCNSAVWKALKTINPAKEKNPWIDEIHEFPVHVPKVSKVIGS*