AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0187820

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0187820 MtrunA17_Chr6g0484901 91.260 492 20 2 1 470 1 491 0.0 866
Msa0187820 MtrunA17_Chr1g0210201 52.754 472 177 7 24 470 17 467 1.70e-165 475
Msa0187820 MtrunA17_Chr7g0275731 46.154 429 175 11 78 470 76 484 2.45e-117 353
Msa0187820 MtrunA17_Chr1g0165201 41.148 418 207 8 82 470 62 469 3.34e-105 321
Msa0187820 MtrunA17_Chr1g0212321 38.266 473 236 10 36 470 32 486 4.56e-105 321
Msa0187820 MtrunA17_Chr8g0390761 42.816 348 173 4 142 463 101 448 2.92e-92 287
Msa0187820 MtrunA17_Chr3g0130931 35.278 360 198 7 144 470 91 448 6.24e-64 213
Msa0187820 MtrunA17_Chr5g0442921 29.754 447 253 11 83 470 109 553 3.32e-61 209
Msa0187820 MtrunA17_Chr3g0095891 32.713 376 205 7 143 470 83 458 6.85e-61 206
Msa0187820 MtrunA17_Chr3g0099901 33.251 403 226 9 105 470 89 485 2.06e-59 202
Msa0187820 MtrunA17_Chr4g0001991 30.788 419 234 10 92 470 115 517 7.61e-57 196
Msa0187820 MtrunA17_Chr5g0435871 32.973 370 205 12 138 470 71 434 1.19e-52 183
Msa0187820 MtrunA17_Chr3g0099891 31.771 384 215 11 120 470 85 454 2.23e-52 183
Msa0187820 MtrunA17_Chr5g0435971 32.703 370 206 12 138 470 68 431 2.39e-51 179
Msa0187820 MtrunA17_Chr5g0419291 31.436 369 212 10 137 470 66 428 9.38e-51 178
Msa0187820 MtrunA17_Chr2g0291201 30.435 414 232 14 100 470 51 451 3.49e-50 177
Msa0187820 MtrunA17_Chr8g0352661 32.213 357 195 13 156 470 85 436 1.62e-47 169
Msa0187820 MtrunA17_Chr8g0386441 30.857 350 202 9 156 470 85 429 1.44e-46 167
Msa0187820 MtrunA17_Chr8g0352671 30.791 354 201 10 156 470 88 436 8.94e-46 165
Msa0187820 MtrunA17_Chr2g0310801 30.056 356 197 9 156 470 89 433 1.01e-43 159
Msa0187820 MtrunA17_Chr6g0487471 30.508 354 203 10 156 470 85 434 1.45e-43 159
Msa0187820 MtrunA17_Chr6g0457401 30.458 371 214 9 138 470 72 436 4.31e-43 157
Msa0187820 MtrunA17_Chr1g0164401 30.919 359 204 11 146 470 80 428 7.21e-43 157
Msa0187820 MtrunA17_Chr2g0310821 28.530 347 205 9 156 470 107 442 1.04e-42 157
Msa0187820 MtrunA17_Chr5g0395871 30.986 355 201 9 153 470 77 424 1.94e-42 155
Msa0187820 MtrunA17_Chr2g0310831 30.919 359 193 12 156 470 93 440 8.78e-42 154
Msa0187820 MtrunA17_Chr3g0133071 31.063 367 210 12 139 470 79 437 2.85e-41 152
Msa0187820 MtrunA17_Chr2g0307461 31.025 361 192 13 162 470 75 430 4.14e-41 152
Msa0187820 MtrunA17_Chr2g0307991 32.312 359 185 14 157 470 59 404 4.27e-41 151
Msa0187820 MtrunA17_Chr3g0087851 28.571 441 249 15 72 470 20 436 2.92e-40 150
Msa0187820 MtrunA17_Chr8g0353261 29.395 347 207 9 155 470 94 433 2.00e-39 147
Msa0187820 MtrunA17_Chr5g0415951 30.141 355 204 13 154 470 86 434 2.77e-39 147
Msa0187820 MtrunA17_Chr4g0061641 29.341 334 193 10 173 470 70 396 2.84e-38 143
Msa0187820 MtrunA17_Chr2g0309561 26.915 457 242 17 96 470 62 508 4.12e-38 145
Msa0187820 MtrunA17_Chr2g0308001 32.410 361 187 15 157 470 58 408 4.78e-38 144
Msa0187820 MtrunA17_Chr5g0395911 29.944 354 203 10 156 470 66 413 5.91e-38 143
Msa0187820 MtrunA17_Chr6g0486981 28.771 358 206 11 155 470 86 436 3.43e-37 141
Msa0187820 MtrunA17_Chr5g0395931 27.723 404 228 13 110 470 53 435 3.30e-35 136
Msa0187820 MtrunA17_Chr2g0333991 27.714 350 207 12 156 465 87 430 5.52e-35 135
Msa0187820 MtrunA17_Chr2g0311451 27.606 355 211 12 156 470 87 435 1.19e-34 134
Msa0187820 MtrunA17_Chr4g0027681 27.384 409 229 16 121 470 37 436 7.09e-29 119
Msa0187820 MtrunA17_Chr4g0023351 34.389 221 105 7 157 339 58 276 1.04e-27 112
Msa0187820 MtrunA17_Chr3g0082281 36.946 203 115 6 276 470 122 319 1.11e-27 112
Msa0187820 MtrunA17_Chr7g0267171 26.823 384 205 15 156 470 89 465 9.30e-27 112
Msa0187820 MtrunA17_Chr7g0249451 26.963 382 203 15 150 470 64 430 1.50e-26 112
Msa0187820 MtrunA17_Chr4g0056211 28.434 415 201 19 143 470 65 470 7.84e-26 110
Msa0187820 MtrunA17_Chr5g0434411 27.451 357 206 13 162 470 86 437 9.10e-26 109
Msa0187820 MtrunA17_Chr1g0189381 24.752 404 240 13 122 470 35 429 9.61e-26 109
Msa0187820 MtrunA17_Chr3g0110191 27.171 357 203 15 162 470 91 438 4.73e-25 107
Msa0187820 MtrunA17_Chr4g0053721 25.208 361 215 12 156 470 79 430 4.12e-24 105
Msa0187820 MtrunA17_Chr3g0112051 28.022 364 175 16 162 470 78 409 7.11e-24 103
Msa0187820 MtrunA17_Chr7g0221681 25.980 408 231 16 122 470 38 433 3.74e-23 102
Msa0187820 MtrunA17_Chr5g0399171 28.199 422 192 22 143 470 65 469 3.77e-23 102
Msa0187820 MtrunA17_Chr7g0239001 26.431 367 203 15 164 470 66 425 3.24e-22 99.0
Msa0187820 MtrunA17_Chr2g0320621 27.100 369 208 12 156 470 76 437 5.20e-22 98.6
Msa0187820 MtrunA17_Chr7g0265331 25.676 370 211 12 156 470 80 440 1.82e-21 97.1
Msa0187820 MtrunA17_Chr5g0401601 24.384 365 213 13 156 470 87 438 2.03e-21 97.4
Msa0187820 MtrunA17_Chr1g0179751 23.514 370 225 9 154 470 105 469 7.40e-21 95.5
Msa0187820 MtrunA17_Chr8g0384731 25.000 360 218 18 156 470 72 424 7.46e-21 94.7
Msa0187820 MtrunA17_Chr2g0312941 26.431 367 207 13 158 466 88 449 1.34e-20 94.0
Msa0187820 MtrunA17_Chr6g0484731 24.274 379 219 16 149 470 183 550 2.11e-20 94.0
Msa0187820 MtrunA17_Chr8g0352681 43.956 91 49 2 144 233 75 164 1.47e-19 85.9
Msa0187820 MtrunA17_Chr5g0434441 23.592 373 202 13 162 470 73 426 1.16e-17 85.1
Msa0187820 MtrunA17_Chr5g0395891 27.090 299 172 12 212 470 20 312 7.20e-17 81.3
Msa0187820 MtrunA17_Chr1g0179091 25.076 331 220 13 155 470 49 366 3.19e-16 80.1
Msa0187820 MtrunA17_Chr3g0130741 23.416 363 219 15 156 470 83 434 1.81e-15 78.6
Msa0187820 MtrunA17_Chr1g0159791 24.100 361 221 14 156 470 56 409 4.70e-15 77.0
Msa0187820 MtrunA17_Chr4g0010951 31.410 156 86 7 156 293 60 212 5.03e-13 70.9
Msa0187820 MtrunA17_Chr8g0352691 27.170 265 153 10 239 470 2 259 2.02e-12 67.4
Msa0187820 MtrunA17_Chr2g0298521 41.250 80 42 2 162 241 120 194 2.28e-12 66.2
Msa0187820 MtrunA17_Chr5g0419151 27.341 267 148 11 241 470 110 367 2.87e-12 68.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0187820 AT3G18490.1 53.360 491 197 6 12 470 10 500 0.0 541
Msa0187820 AT1G25510.1 51.592 471 190 6 29 470 22 483 8.89e-174 497
Msa0187820 AT1G01300.1 46.585 410 178 9 93 470 84 484 4.78e-113 342
Msa0187820 AT3G61820.1 46.602 412 169 11 100 470 82 483 3.57e-111 337
Msa0187820 AT3G20015.1 39.151 424 222 6 75 470 55 470 1.86e-105 322
Msa0187820 AT5G10760.1 33.673 392 218 8 105 470 89 464 1.43e-60 205
Msa0187820 AT2G03200.1 34.819 359 189 11 154 470 103 458 1.51e-59 202
Msa0187820 AT3G59080.1 31.002 429 229 13 107 470 106 532 2.12e-58 201
Msa0187820 AT1G64830.1 34.270 356 188 12 153 470 81 428 4.43e-56 192
Msa0187820 AT5G10770.1 33.500 400 218 12 105 470 88 473 6.96e-56 193
Msa0187820 AT2G42980.1 29.812 426 237 13 99 470 107 524 8.64e-54 189
Msa0187820 AT1G79720.1 30.238 420 229 12 96 470 82 482 4.80e-52 183
Msa0187820 AT5G33340.1 33.708 356 192 12 153 470 85 434 2.86e-50 177
Msa0187820 AT3G59080.2 29.787 423 206 12 107 470 106 496 8.56e-50 177
Msa0187820 AT3G54400.1 32.687 361 200 14 144 470 73 424 2.09e-43 158
Msa0187820 AT2G35615.1 27.335 439 250 15 84 470 22 443 2.14e-41 153
Msa0187820 AT3G25700.1 28.472 432 246 12 93 470 27 449 3.47e-41 153
Msa0187820 AT5G07030.1 30.663 362 205 13 146 470 102 454 2.32e-40 150
Msa0187820 AT1G31450.1 29.797 443 223 19 82 470 33 441 7.66e-40 149
Msa0187820 AT1G09750.1 31.593 364 207 15 144 470 90 448 3.35e-36 139
Msa0187820 AT2G28010.1 28.035 346 197 10 158 470 65 391 8.86e-34 131
Msa0187820 AT4G30030.1 27.988 343 216 11 158 470 78 419 5.10e-33 130
Msa0187820 AT5G36260.1 26.799 403 227 15 123 470 44 433 5.82e-33 130
Msa0187820 AT2G28040.1 28.161 348 195 10 157 470 64 390 3.30e-31 124
Msa0187820 AT3G02740.1 27.518 407 228 17 118 470 46 439 4.45e-31 125
Msa0187820 AT2G28040.2 28.161 348 195 10 157 470 55 381 4.54e-31 124
Msa0187820 AT2G28225.1 26.781 351 199 10 158 470 67 397 6.97e-31 123
Msa0187820 AT3G12700.1 26.558 369 223 13 147 470 95 460 1.90e-30 123
Msa0187820 AT2G28220.1 26.629 353 197 10 158 470 67 397 3.78e-30 121
Msa0187820 AT3G02740.2 27.596 366 201 15 158 470 10 364 1.08e-29 120
Msa0187820 AT4G30040.1 27.011 348 209 10 158 465 85 427 1.10e-29 120
Msa0187820 AT2G28030.1 27.035 344 203 8 158 470 61 387 1.49e-29 119
Msa0187820 AT2G23945.1 24.646 353 226 8 158 470 96 448 1.66e-26 112
Msa0187820 AT5G37540.1 28.933 356 205 12 162 470 84 438 1.84e-26 111
Msa0187820 AT1G05840.1 26.341 410 233 17 118 470 41 438 2.01e-26 112
Msa0187820 AT2G39710.1 29.412 374 188 19 162 470 69 431 2.35e-26 111
Msa0187820 AT3G50050.1 25.348 359 211 12 156 470 91 436 2.92e-26 112
Msa0187820 AT5G02190.1 27.297 370 194 16 167 470 82 442 2.10e-25 108
Msa0187820 AT5G22850.1 26.287 369 211 14 156 470 79 440 1.11e-24 107
Msa0187820 AT1G65240.1 24.796 367 212 14 156 470 72 426 1.58e-24 106
Msa0187820 AT1G66180.1 27.424 361 200 12 162 470 76 426 2.21e-24 105
Msa0187820 AT2G36670.2 25.474 369 212 11 156 470 98 457 1.17e-23 103
Msa0187820 AT2G36670.4 25.341 367 211 11 158 470 34 391 2.55e-23 102
Msa0187820 AT2G36670.3 25.341 367 211 11 158 470 34 391 2.55e-23 102
Msa0187820 AT2G36670.1 25.341 367 211 11 158 470 105 462 3.67e-23 102
Msa0187820 AT5G43100.1 24.581 358 213 16 156 470 74 417 1.06e-22 101
Msa0187820 AT5G43100.2 24.302 358 214 15 156 470 74 417 1.24e-22 100
Msa0187820 AT3G52500.1 26.371 383 211 16 156 470 88 467 6.43e-22 98.6
Msa0187820 AT3G25700.2 33.862 189 104 7 93 268 27 207 1.89e-21 95.9
Msa0187820 AT4G33490.2 25.348 359 219 16 156 470 58 411 3.82e-21 95.9
Msa0187820 AT4G33490.1 29.796 245 140 10 156 372 55 295 2.15e-19 90.5
Msa0187820 AT5G45120.1 24.747 396 215 16 158 470 83 478 1.20e-18 89.0
Msa0187820 AT3G42550.1 23.671 414 266 15 71 470 12 389 2.13e-18 87.8
Msa0187820 AT1G08210.3 23.626 364 224 11 156 470 130 488 2.73e-17 84.7
Msa0187820 AT1G08210.4 23.626 364 224 11 156 470 116 474 3.39e-17 84.7
Msa0187820 AT1G08210.1 23.626 364 224 11 156 470 82 440 4.11e-17 84.3
Msa0187820 AT1G77480.2 23.410 393 232 18 134 470 40 419 6.54e-17 83.2
Msa0187820 AT1G77480.1 23.797 395 228 18 134 470 40 419 7.06e-17 83.2
Msa0187820 AT4G16563.1 27.972 429 198 16 143 470 73 491 1.60e-16 82.4
Msa0187820 AT1G49050.1 20.800 375 230 17 156 470 201 568 4.87e-16 81.3
Msa0187820 AT1G49050.3 23.019 265 160 10 156 380 201 461 1.07e-15 79.7
Msa0187820 AT3G51350.1 22.253 364 219 11 158 470 102 452 1.28e-15 79.7
Msa0187820 AT1G49050.2 20.107 373 231 16 158 470 30 395 1.42e-15 79.0
Msa0187820 AT5G10080.1 25.964 389 204 17 144 470 87 453 8.96e-15 77.0
Msa0187820 AT4G12920.1 24.100 361 225 11 143 470 43 387 1.18e-14 75.9
Msa0187820 AT1G08210.2 24.118 340 204 11 156 446 82 416 4.83e-14 74.7

Find 104 sgRNAs with CRISPR-Local

Find 256 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTTCTTCATTGTCTATTAT+TGG 0.121880 2_1:-2579863 Msa0187820:CDS
TGTCAATCACTCGAACTTTC+TGG 0.181123 2_1:-2580588 Msa0187820:CDS
TCAAGAAGATTATTGTTATT+AGG 0.186341 2_1:+2581071 None:intergenic
TGTTATTAGGGTTAAAATTA+AGG 0.205225 2_1:+2581084 None:intergenic
GCGGCGGCGGGCGATGGTTC+TGG 0.242006 2_1:-2580169 Msa0187820:CDS
CAAGAAGATTATTGTTATTA+GGG 0.244824 2_1:+2581072 None:intergenic
TGTCAAATTTGCAAAGTGTT+AGG 0.266037 2_1:-2580000 Msa0187820:CDS
ACACCAGTCTCCTCCGGTTC+TGG 0.294317 2_1:-2580807 Msa0187820:CDS
GAGGAAGTTGGGTCGTATAT+TGG 0.294568 2_1:+2580648 None:intergenic
TGGGCAGATGGTTGCTGTTC+CGG 0.300297 2_1:-2580191 Msa0187820:CDS
AGCACTGAAACAGTCTCTTT+TGG 0.304461 2_1:-2580498 Msa0187820:CDS
AATCTTGCTAATAATTTAGT+AGG 0.325828 2_1:-2579806 Msa0187820:CDS
GGTTGCTGTTCCGGCGGCGG+CGG 0.328873 2_1:-2580182 Msa0187820:CDS
TGTTGAAGCTCGGTTTGAAC+CGG 0.347290 2_1:+2580843 None:intergenic
AGAACCATGTAGTAGGGTTT+TGG 0.352914 2_1:+2580738 None:intergenic
CGTATATTGGGTCAGTTTGT+TGG 0.365812 2_1:+2580661 None:intergenic
AAAAGCAAAGCAAAACTTTC+CGG 0.372345 2_1:+2579895 None:intergenic
ACACAGGTAGTGACGTAAAC+TGG 0.375480 2_1:-2580713 Msa0187820:CDS
GGCGAAGAATTACTTGATTC+CGG 0.382640 2_1:-2579927 Msa0187820:CDS
GAATAAGGTTTGGAGTTTGC+CGG 0.387350 2_1:-2579948 Msa0187820:CDS
GTTCCGGCGGCGGCGGGCGA+TGG 0.395405 2_1:-2580175 Msa0187820:CDS
GTCAAATTTGCAAAGTGTTA+GGG 0.398124 2_1:-2579999 Msa0187820:CDS
AGGAAGTTGGGTCGTATATT+GGG 0.404786 2_1:+2580649 None:intergenic
TGATAGGTTGATGAGGAAGT+TGG 0.410689 2_1:+2580636 None:intergenic
GCCGGAGCCGGAGCCAGAAC+CGG 0.410875 2_1:+2580794 None:intergenic
GTTGCTGTTCCGGCGGCGGC+GGG 0.413651 2_1:-2580181 Msa0187820:CDS
TTGACTCTGTAACCGCGTTA+TGG 0.426385 2_1:+2580114 None:intergenic
ACGAATAAACCTCGTTATCA+TGG 0.427575 2_1:+2580436 None:intergenic
GAGTCTAGCTTGCATAAGTT+TGG 0.427941 2_1:+2580947 None:intergenic
GTCTCCTCCGGTTCTGGCTC+CGG 0.432085 2_1:-2580801 Msa0187820:CDS
TGGTTGGCCTATACCTAGTC+TGG 0.437865 2_1:+2580761 None:intergenic
TAAGTTTGGTATAGTCTTTG+TGG 0.439184 2_1:+2580961 None:intergenic
TCCGGTTCTGGCTCCGGCTC+CGG 0.439544 2_1:-2580795 Msa0187820:CDS
ATGATTCTAGATGAGGAAGT+TGG 0.439677 2_1:+2581167 None:intergenic
GCGGGCGATGGTTCTGGAAG+TGG 0.441038 2_1:-2580163 Msa0187820:CDS
TTGACATTGTTCTGCTTCAC+AGG 0.443922 2_1:+2580605 None:intergenic
AATGAGAAGTGATAAGTTGA+AGG 0.458328 2_1:+2581214 None:intergenic
CTGTTTAGTTGATCGTAACT+CGG 0.471197 2_1:-2580335 Msa0187820:CDS
GAGCCAGAACCGGAGGAGAC+TGG 0.471361 2_1:+2580804 None:intergenic
TATTTCTCCAGACTAGGTAT+AGG 0.472269 2_1:-2580768 Msa0187820:CDS
CTCTCTACACCAGTCTCCTC+CGG 0.474159 2_1:-2580813 Msa0187820:CDS
CGGTGCAAGTACTGAATCAC+CGG 0.477993 2_1:+2580276 None:intergenic
AACCGTGTTGCTATCGGTTG+TGG 0.479426 2_1:-2580459 Msa0187820:CDS
TTCACAGGCCACGGGTTGAT+AGG 0.480371 2_1:+2580620 None:intergenic
TTGTTCTGCTTCACAGGCCA+CGG 0.483509 2_1:+2580611 None:intergenic
GATGGTTGCTGTTCCGGCGG+CGG 0.488784 2_1:-2580185 Msa0187820:CDS
GATAGGTTGATGAGGAAGTT+GGG 0.493074 2_1:+2580637 None:intergenic
GGCGATGGTTCTGGAAGTGG+TGG 0.494014 2_1:-2580160 Msa0187820:CDS
TGTCAATACCAAGTTTCTTA+CGG 0.496571 2_1:-2580549 Msa0187820:CDS
AGCCATCACCGTAAGAAACT+TGG 0.503849 2_1:+2580541 None:intergenic
CTAGATGAGGAAGTTGGAGA+TGG 0.505794 2_1:+2581173 None:intergenic
GAGATCTTCTTGTTGAAGCT+CGG 0.507550 2_1:+2580833 None:intergenic
GAACCATCGCCCGCCGCCGC+CGG 0.511271 2_1:+2580172 None:intergenic
AACTTTCCGGCAGAATCCAC+CGG 0.512170 2_1:+2579908 None:intergenic
GAACTTTCTGGTTGTCGTGA+TGG 0.516786 2_1:-2580576 Msa0187820:CDS
GGAGAAATACTCGCCGGAGC+CGG 0.522013 2_1:+2580782 None:intergenic
GTTGAAGCTCGGTTTGAACC+GGG 0.525615 2_1:+2580844 None:intergenic
CTCACTGGTATAAGTGTTGG+TGG 0.526614 2_1:-2580211 Msa0187820:CDS
TTGATTCCGGTGGATTCTGC+CGG 0.531084 2_1:-2579914 Msa0187820:CDS
TGTTTAGTTGATCGTAACTC+GGG 0.531571 2_1:-2580334 Msa0187820:CDS
TGTGTCAAGAACCATGTAGT+AGG 0.533525 2_1:+2580731 None:intergenic
GGTTTGAACCGGGTGGAGAT+CGG 0.536393 2_1:+2580854 None:intergenic
TGTTGGTAACATTCTGTGCA+TGG 0.537558 2_1:+2580678 None:intergenic
TTGAGGAAGAGATATCAAGA+AGG 0.539178 2_1:+2581126 None:intergenic
AGCAACACGGTTCACTGACC+CGG 0.539290 2_1:+2580470 None:intergenic
TACCAAGTTTCTTACGGTGA+TGG 0.540230 2_1:-2580543 Msa0187820:CDS
TGTTCTGCTTCACAGGCCAC+GGG 0.543088 2_1:+2580612 None:intergenic
TCAGTGAACCGTGTTGCTAT+CGG 0.544791 2_1:-2580465 Msa0187820:CDS
ATTCTGTGCATGGTTTGCAT+TGG 0.547691 2_1:+2580688 None:intergenic
TGAGGAAGAGATATCAAGAA+GGG 0.550503 2_1:+2581127 None:intergenic
ACTCTCTGATTCTTGAGCAT+CGG 0.554931 2_1:+2580256 None:intergenic
GGTGGGATTATTGTTGACTC+TGG 0.555801 2_1:-2580142 Msa0187820:CDS
TCAAGAAGGGAAGTGTGTGA+AGG 0.558101 2_1:+2581140 None:intergenic
GAGTTTAACTCGGTTCAACC+CGG 0.566822 2_1:-2580295 Msa0187820:CDS
GTCAACTCTTGAGTTTAACT+CGG 0.567369 2_1:-2580305 Msa0187820:CDS
GGAGCCGGAGCCAGAACCGG+AGG 0.572106 2_1:+2580797 None:intergenic
CCAAAATGAAAAGCCACAGT+TGG 0.573369 2_1:+2579974 None:intergenic
GCGATGGTTCTGGAAGTGGT+GGG 0.576981 2_1:-2580159 Msa0187820:CDS
AGGAGTAATGATTCTAGATG+AGG 0.577172 2_1:+2581160 None:intergenic
TATAAGTGTTGGTGGGCAGA+TGG 0.579265 2_1:-2580203 Msa0187820:CDS
ACCACCAAAACCCTACTACA+TGG 0.585191 2_1:-2580742 Msa0187820:CDS
TACTACATGGTTCTTGACAC+AGG 0.586619 2_1:-2580729 Msa0187820:CDS
GTGTCAAGAACCATGTAGTA+GGG 0.589979 2_1:+2580732 None:intergenic
AAGTGTTAGGGTTCCAACTG+TGG 0.596932 2_1:-2579987 Msa0187820:CDS
ATTGGAAATGTTCAACAACA+AGG 0.601781 2_1:-2579845 Msa0187820:CDS
ACTCTGTAACCGCGTTATGG+CGG 0.603076 2_1:+2580117 None:intergenic
GGAATCAAGTAATTCTTCGC+CGG 0.608752 2_1:+2579929 None:intergenic
CAAGAAAACCGATCTCCACC+CGG 0.616953 2_1:-2580862 Msa0187820:CDS
CGGTTGTGGCCATGATAACG+AGG 0.619718 2_1:-2580445 Msa0187820:intron
GGTGATGGCTCATTCACTGT+AGG 0.625401 2_1:-2580528 Msa0187820:CDS
CAACTCACTGGTATAAGTGT+TGG 0.628270 2_1:-2580214 Msa0187820:CDS
TTGGAAATGTTCAACAACAA+GGG 0.629226 2_1:-2579844 Msa0187820:CDS
ACTCTGGAACCGCCATAACG+CGG 0.629561 2_1:-2580126 Msa0187820:CDS
CTCATCAACCTATCAACCCG+TGG 0.637052 2_1:-2580628 Msa0187820:CDS
TAGTCTGGAGAAATACTCGC+CGG 0.638745 2_1:+2580776 None:intergenic
CACGGGTTGATAGGTTGATG+AGG 0.644825 2_1:+2580629 None:intergenic
GCAGATGGTTGCTGTTCCGG+CGG 0.655706 2_1:-2580188 Msa0187820:CDS
GGCGAGTATTTCTCCAGACT+AGG 0.673783 2_1:-2580774 Msa0187820:CDS
TCACTGGTATAAGTGTTGGT+GGG 0.680307 2_1:-2580210 Msa0187820:CDS
ATAGACAATGAAGAACTCGT+CGG 0.681138 2_1:+2579869 None:intergenic
GGTGCAAGTACTGAATCACC+GGG 0.690821 2_1:+2580277 None:intergenic
GGCCACAACCGATAGCAACA+CGG 0.691870 2_1:+2580457 None:intergenic
GAAGCTCGGTTTGAACCGGG+TGG 0.706106 2_1:+2580847 None:intergenic
GAAGAATTACTTGATTCCGG+TGG 0.707703 2_1:-2579924 Msa0187820:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTTAAAATTAAGGACTTTTT+TGG + chr2_1:2579932-2579951 None:intergenic 20.0%
!! TGTTATTAGGGTTAAAATTA+AGG + chr2_1:2579942-2579961 None:intergenic 20.0%
!!! CAAGAAGATTATTGTTATTA+GGG + chr2_1:2579954-2579973 None:intergenic 20.0%
!!! TCAAGAAGATTATTGTTATT+AGG + chr2_1:2579955-2579974 None:intergenic 20.0%
!!! AAAAATGGATTTTTCGTTTA+GGG + chr2_1:2579983-2580002 None:intergenic 20.0%
!!! AAAAAATGGATTTTTCGTTT+AGG + chr2_1:2579984-2580003 None:intergenic 20.0%
!! AATCTTGCTAATAATTTAGT+AGG - chr2_1:2581217-2581236 Msa0187820:CDS 20.0%
!!! GTTAAAATTAAGGACTTTTT+TGG + chr2_1:2579932-2579951 None:intergenic 20.0%
!! TGTTATTAGGGTTAAAATTA+AGG + chr2_1:2579942-2579961 None:intergenic 20.0%
!!! CAAGAAGATTATTGTTATTA+GGG + chr2_1:2579954-2579973 None:intergenic 20.0%
!!! TCAAGAAGATTATTGTTATT+AGG + chr2_1:2579955-2579974 None:intergenic 20.0%
!!! AAAAATGGATTTTTCGTTTA+GGG + chr2_1:2579983-2580002 None:intergenic 20.0%
!!! AAAAAATGGATTTTTCGTTT+AGG + chr2_1:2579984-2580003 None:intergenic 20.0%
!! AATCTTGCTAATAATTTAGT+AGG - chr2_1:2581217-2581236 Msa0187820:CDS 20.0%
!!! AATTAAGGACTTTTTTGGTT+TGG + chr2_1:2579927-2579946 None:intergenic 25.0%
! AGATGAAGAGTTTGAAAAAA+TGG + chr2_1:2579998-2580017 None:intergenic 25.0%
!!! TTTTATTTTGTTCAACTCAC+TGG - chr2_1:2580797-2580816 Msa0187820:CDS 25.0%
!!! TTTTCATTTTGGTGAGAATA+AGG - chr2_1:2581060-2581079 Msa0187820:CDS 25.0%
! AGTTCTTCATTGTCTATTAT+TGG - chr2_1:2581160-2581179 Msa0187820:CDS 25.0%
!!! AATTAAGGACTTTTTTGGTT+TGG + chr2_1:2579927-2579946 None:intergenic 25.0%
! AGATGAAGAGTTTGAAAAAA+TGG + chr2_1:2579998-2580017 None:intergenic 25.0%
!!! TTTTATTTTGTTCAACTCAC+TGG - chr2_1:2580797-2580816 Msa0187820:CDS 25.0%
!!! TTTTCATTTTGGTGAGAATA+AGG - chr2_1:2581060-2581079 Msa0187820:CDS 25.0%
! AGTTCTTCATTGTCTATTAT+TGG - chr2_1:2581160-2581179 Msa0187820:CDS 25.0%
!!! GAAGGAAATAAGTGCTTTTT+TGG + chr2_1:2579794-2579813 None:intergenic 30.0%
! AATGAGAAGTGATAAGTTGA+AGG + chr2_1:2579812-2579831 None:intergenic 30.0%
!!! TTTTTTGGTTTGGTGAATTG+AGG + chr2_1:2579917-2579936 None:intergenic 30.0%
! TCATTGATGAGATTTTCTCT+TGG + chr2_1:2580039-2580058 None:intergenic 30.0%
TAAGTTTGGTATAGTCTTTG+TGG + chr2_1:2580065-2580084 None:intergenic 30.0%
TGTCAATACCAAGTTTCTTA+CGG - chr2_1:2580474-2580493 Msa0187820:CDS 30.0%
! TGTCAAATTTGCAAAGTGTT+AGG - chr2_1:2581023-2581042 Msa0187820:CDS 30.0%
! GTCAAATTTGCAAAGTGTTA+GGG - chr2_1:2581024-2581043 Msa0187820:CDS 30.0%
!! ATTTTGGTGAGAATAAGGTT+TGG - chr2_1:2581065-2581084 Msa0187820:CDS 30.0%
AAAAGCAAAGCAAAACTTTC+CGG + chr2_1:2581131-2581150 None:intergenic 30.0%
ATTGGAAATGTTCAACAACA+AGG - chr2_1:2581178-2581197 Msa0187820:CDS 30.0%
TTGGAAATGTTCAACAACAA+GGG - chr2_1:2581179-2581198 Msa0187820:CDS 30.0%
!!! GAAGGAAATAAGTGCTTTTT+TGG + chr2_1:2579794-2579813 None:intergenic 30.0%
! AATGAGAAGTGATAAGTTGA+AGG + chr2_1:2579812-2579831 None:intergenic 30.0%
!!! TTTTTTGGTTTGGTGAATTG+AGG + chr2_1:2579917-2579936 None:intergenic 30.0%
! TCATTGATGAGATTTTCTCT+TGG + chr2_1:2580039-2580058 None:intergenic 30.0%
TAAGTTTGGTATAGTCTTTG+TGG + chr2_1:2580065-2580084 None:intergenic 30.0%
TGTCAATACCAAGTTTCTTA+CGG - chr2_1:2580474-2580493 Msa0187820:CDS 30.0%
! TGTCAAATTTGCAAAGTGTT+AGG - chr2_1:2581023-2581042 Msa0187820:CDS 30.0%
! GTCAAATTTGCAAAGTGTTA+GGG - chr2_1:2581024-2581043 Msa0187820:CDS 30.0%
!! ATTTTGGTGAGAATAAGGTT+TGG - chr2_1:2581065-2581084 Msa0187820:CDS 30.0%
AAAAGCAAAGCAAAACTTTC+CGG + chr2_1:2581131-2581150 None:intergenic 30.0%
ATTGGAAATGTTCAACAACA+AGG - chr2_1:2581178-2581197 Msa0187820:CDS 30.0%
TTGGAAATGTTCAACAACAA+GGG - chr2_1:2581179-2581198 Msa0187820:CDS 30.0%
ATGATTCTAGATGAGGAAGT+TGG + chr2_1:2579859-2579878 None:intergenic 35.0%
AGGAGTAATGATTCTAGATG+AGG + chr2_1:2579866-2579885 None:intergenic 35.0%
TGAGGAAGAGATATCAAGAA+GGG + chr2_1:2579899-2579918 None:intergenic 35.0%
TTGAGGAAGAGATATCAAGA+AGG + chr2_1:2579900-2579919 None:intergenic 35.0%
TATTTCTCCAGACTAGGTAT+AGG - chr2_1:2580255-2580274 Msa0187820:CDS 35.0%
ACGAATAAACCTCGTTATCA+TGG + chr2_1:2580590-2580609 None:intergenic 35.0%
! TGATAACGAGGTTTATTCGT+CGG - chr2_1:2580590-2580609 Msa0187820:CDS 35.0%
CTGTTTAGTTGATCGTAACT+CGG - chr2_1:2580688-2580707 Msa0187820:CDS 35.0%
TGTTTAGTTGATCGTAACTC+GGG - chr2_1:2580689-2580708 Msa0187820:CDS 35.0%
GTCAACTCTTGAGTTTAACT+CGG - chr2_1:2580718-2580737 Msa0187820:CDS 35.0%
ATAGACAATGAAGAACTCGT+CGG + chr2_1:2581157-2581176 None:intergenic 35.0%
ATGATTCTAGATGAGGAAGT+TGG + chr2_1:2579859-2579878 None:intergenic 35.0%
AGGAGTAATGATTCTAGATG+AGG + chr2_1:2579866-2579885 None:intergenic 35.0%
TGAGGAAGAGATATCAAGAA+GGG + chr2_1:2579899-2579918 None:intergenic 35.0%
TTGAGGAAGAGATATCAAGA+AGG + chr2_1:2579900-2579919 None:intergenic 35.0%
TATTTCTCCAGACTAGGTAT+AGG - chr2_1:2580255-2580274 Msa0187820:CDS 35.0%
ACGAATAAACCTCGTTATCA+TGG + chr2_1:2580590-2580609 None:intergenic 35.0%
! TGATAACGAGGTTTATTCGT+CGG - chr2_1:2580590-2580609 Msa0187820:CDS 35.0%
CTGTTTAGTTGATCGTAACT+CGG - chr2_1:2580688-2580707 Msa0187820:CDS 35.0%
TGTTTAGTTGATCGTAACTC+GGG - chr2_1:2580689-2580708 Msa0187820:CDS 35.0%
GTCAACTCTTGAGTTTAACT+CGG - chr2_1:2580718-2580737 Msa0187820:CDS 35.0%
ATAGACAATGAAGAACTCGT+CGG + chr2_1:2581157-2581176 None:intergenic 35.0%
! GAGTCTAGCTTGCATAAGTT+TGG + chr2_1:2580079-2580098 None:intergenic 40.0%
GAGATCTTCTTGTTGAAGCT+CGG + chr2_1:2580193-2580212 None:intergenic 40.0%
! AGAACCATGTAGTAGGGTTT+TGG + chr2_1:2580288-2580307 None:intergenic 40.0%
GTGTCAAGAACCATGTAGTA+GGG + chr2_1:2580294-2580313 None:intergenic 40.0%
TGTGTCAAGAACCATGTAGT+AGG + chr2_1:2580295-2580314 None:intergenic 40.0%
TACTACATGGTTCTTGACAC+AGG - chr2_1:2580294-2580313 Msa0187820:CDS 40.0%
! ATTCTGTGCATGGTTTGCAT+TGG + chr2_1:2580338-2580357 None:intergenic 40.0%
!! TGTTGGTAACATTCTGTGCA+TGG + chr2_1:2580348-2580367 None:intergenic 40.0%
CGTATATTGGGTCAGTTTGT+TGG + chr2_1:2580365-2580384 None:intergenic 40.0%
AGGAAGTTGGGTCGTATATT+GGG + chr2_1:2580377-2580396 None:intergenic 40.0%
!! GATAGGTTGATGAGGAAGTT+GGG + chr2_1:2580389-2580408 None:intergenic 40.0%
!! TGATAGGTTGATGAGGAAGT+TGG + chr2_1:2580390-2580409 None:intergenic 40.0%
TTGACATTGTTCTGCTTCAC+AGG + chr2_1:2580421-2580440 None:intergenic 40.0%
TGTCAATCACTCGAACTTTC+TGG - chr2_1:2580435-2580454 Msa0187820:intron 40.0%
TACCAAGTTTCTTACGGTGA+TGG - chr2_1:2580480-2580499 Msa0187820:CDS 40.0%
!! AGCACTGAAACAGTCTCTTT+TGG - chr2_1:2580525-2580544 Msa0187820:CDS 40.0%
!! ACAGTCTCTTTTGGAAACTC+CGG - chr2_1:2580534-2580553 Msa0187820:CDS 40.0%
ACTCTCTGATTCTTGAGCAT+CGG + chr2_1:2580770-2580789 None:intergenic 40.0%
CAACTCACTGGTATAAGTGT+TGG - chr2_1:2580809-2580828 Msa0187820:CDS 40.0%
!! TCACTGGTATAAGTGTTGGT+GGG - chr2_1:2580813-2580832 Msa0187820:CDS 40.0%
CCAAAATGAAAAGCCACAGT+TGG + chr2_1:2581052-2581071 None:intergenic 40.0%
! CCAACTGTGGCTTTTCATTT+TGG - chr2_1:2581049-2581068 Msa0187820:CDS 40.0%
! GAATAAGGTTTGGAGTTTGC+CGG - chr2_1:2581075-2581094 Msa0187820:CDS 40.0%
GGAATCAAGTAATTCTTCGC+CGG + chr2_1:2581097-2581116 None:intergenic 40.0%
GGCGAAGAATTACTTGATTC+CGG - chr2_1:2581096-2581115 Msa0187820:CDS 40.0%
GAAGAATTACTTGATTCCGG+TGG - chr2_1:2581099-2581118 Msa0187820:CDS 40.0%
! GAGTCTAGCTTGCATAAGTT+TGG + chr2_1:2580079-2580098 None:intergenic 40.0%
GAGATCTTCTTGTTGAAGCT+CGG + chr2_1:2580193-2580212 None:intergenic 40.0%
! AGAACCATGTAGTAGGGTTT+TGG + chr2_1:2580288-2580307 None:intergenic 40.0%
GTGTCAAGAACCATGTAGTA+GGG + chr2_1:2580294-2580313 None:intergenic 40.0%
TGTGTCAAGAACCATGTAGT+AGG + chr2_1:2580295-2580314 None:intergenic 40.0%
TACTACATGGTTCTTGACAC+AGG - chr2_1:2580294-2580313 Msa0187820:CDS 40.0%
! ATTCTGTGCATGGTTTGCAT+TGG + chr2_1:2580338-2580357 None:intergenic 40.0%
!! TGTTGGTAACATTCTGTGCA+TGG + chr2_1:2580348-2580367 None:intergenic 40.0%
CGTATATTGGGTCAGTTTGT+TGG + chr2_1:2580365-2580384 None:intergenic 40.0%
AGGAAGTTGGGTCGTATATT+GGG + chr2_1:2580377-2580396 None:intergenic 40.0%
!! GATAGGTTGATGAGGAAGTT+GGG + chr2_1:2580389-2580408 None:intergenic 40.0%
!! TGATAGGTTGATGAGGAAGT+TGG + chr2_1:2580390-2580409 None:intergenic 40.0%
TTGACATTGTTCTGCTTCAC+AGG + chr2_1:2580421-2580440 None:intergenic 40.0%
TGTCAATCACTCGAACTTTC+TGG - chr2_1:2580435-2580454 Msa0187820:intron 40.0%
TACCAAGTTTCTTACGGTGA+TGG - chr2_1:2580480-2580499 Msa0187820:CDS 40.0%
!! AGCACTGAAACAGTCTCTTT+TGG - chr2_1:2580525-2580544 Msa0187820:CDS 40.0%
!! ACAGTCTCTTTTGGAAACTC+CGG - chr2_1:2580534-2580553 Msa0187820:CDS 40.0%
ACTCTCTGATTCTTGAGCAT+CGG + chr2_1:2580770-2580789 None:intergenic 40.0%
CAACTCACTGGTATAAGTGT+TGG - chr2_1:2580809-2580828 Msa0187820:CDS 40.0%
!! TCACTGGTATAAGTGTTGGT+GGG - chr2_1:2580813-2580832 Msa0187820:CDS 40.0%
CCAAAATGAAAAGCCACAGT+TGG + chr2_1:2581052-2581071 None:intergenic 40.0%
! CCAACTGTGGCTTTTCATTT+TGG - chr2_1:2581049-2581068 Msa0187820:CDS 40.0%
! GAATAAGGTTTGGAGTTTGC+CGG - chr2_1:2581075-2581094 Msa0187820:CDS 40.0%
GGAATCAAGTAATTCTTCGC+CGG + chr2_1:2581097-2581116 None:intergenic 40.0%
GGCGAAGAATTACTTGATTC+CGG - chr2_1:2581096-2581115 Msa0187820:CDS 40.0%
GAAGAATTACTTGATTCCGG+TGG - chr2_1:2581099-2581118 Msa0187820:CDS 40.0%
CTAGATGAGGAAGTTGGAGA+TGG + chr2_1:2579853-2579872 None:intergenic 45.0%
! TCAAGAAGGGAAGTGTGTGA+AGG + chr2_1:2579886-2579905 None:intergenic 45.0%
! TGTTGAAGCTCGGTTTGAAC+CGG + chr2_1:2580183-2580202 None:intergenic 45.0%
TAGTCTGGAGAAATACTCGC+CGG + chr2_1:2580250-2580269 None:intergenic 45.0%
!! TGTAGTAGGGTTTTGGTGGT+TGG + chr2_1:2580281-2580300 None:intergenic 45.0%
ACCACCAAAACCCTACTACA+TGG - chr2_1:2580281-2580300 Msa0187820:CDS 45.0%
!! ACCATGTAGTAGGGTTTTGG+TGG + chr2_1:2580285-2580304 None:intergenic 45.0%
ACACAGGTAGTGACGTAAAC+TGG - chr2_1:2580310-2580329 Msa0187820:CDS 45.0%
GAGGAAGTTGGGTCGTATAT+TGG + chr2_1:2580378-2580397 None:intergenic 45.0%
!! GAACTTTCTGGTTGTCGTGA+TGG - chr2_1:2580447-2580466 Msa0187820:CDS 45.0%
AGCCATCACCGTAAGAAACT+TGG + chr2_1:2580485-2580504 None:intergenic 45.0%
!! CAGTCTCTTTTGGAAACTCC+GGG - chr2_1:2580535-2580554 Msa0187820:CDS 45.0%
! TCAGTGAACCGTGTTGCTAT+CGG - chr2_1:2580558-2580577 Msa0187820:CDS 45.0%
GAGTTTAACTCGGTTCAACC+CGG - chr2_1:2580728-2580747 Msa0187820:CDS 45.0%
!! CTCACTGGTATAAGTGTTGG+TGG - chr2_1:2580812-2580831 Msa0187820:CDS 45.0%
!! TATAAGTGTTGGTGGGCAGA+TGG - chr2_1:2580820-2580839 Msa0187820:CDS 45.0%
!! GGTGGGATTATTGTTGACTC+TGG - chr2_1:2580881-2580900 Msa0187820:CDS 45.0%
TTGACTCTGTAACCGCGTTA+TGG + chr2_1:2580912-2580931 None:intergenic 45.0%
AAGTGTTAGGGTTCCAACTG+TGG - chr2_1:2581036-2581055 Msa0187820:CDS 45.0%
CTAGATGAGGAAGTTGGAGA+TGG + chr2_1:2579853-2579872 None:intergenic 45.0%
! TCAAGAAGGGAAGTGTGTGA+AGG + chr2_1:2579886-2579905 None:intergenic 45.0%
! TGTTGAAGCTCGGTTTGAAC+CGG + chr2_1:2580183-2580202 None:intergenic 45.0%
TAGTCTGGAGAAATACTCGC+CGG + chr2_1:2580250-2580269 None:intergenic 45.0%
!! TGTAGTAGGGTTTTGGTGGT+TGG + chr2_1:2580281-2580300 None:intergenic 45.0%
ACCACCAAAACCCTACTACA+TGG - chr2_1:2580281-2580300 Msa0187820:CDS 45.0%
!! ACCATGTAGTAGGGTTTTGG+TGG + chr2_1:2580285-2580304 None:intergenic 45.0%
ACACAGGTAGTGACGTAAAC+TGG - chr2_1:2580310-2580329 Msa0187820:CDS 45.0%
GAGGAAGTTGGGTCGTATAT+TGG + chr2_1:2580378-2580397 None:intergenic 45.0%
!! GAACTTTCTGGTTGTCGTGA+TGG - chr2_1:2580447-2580466 Msa0187820:CDS 45.0%
AGCCATCACCGTAAGAAACT+TGG + chr2_1:2580485-2580504 None:intergenic 45.0%
!! CAGTCTCTTTTGGAAACTCC+GGG - chr2_1:2580535-2580554 Msa0187820:CDS 45.0%
! TCAGTGAACCGTGTTGCTAT+CGG - chr2_1:2580558-2580577 Msa0187820:CDS 45.0%
GAGTTTAACTCGGTTCAACC+CGG - chr2_1:2580728-2580747 Msa0187820:CDS 45.0%
!! CTCACTGGTATAAGTGTTGG+TGG - chr2_1:2580812-2580831 Msa0187820:CDS 45.0%
!! TATAAGTGTTGGTGGGCAGA+TGG - chr2_1:2580820-2580839 Msa0187820:CDS 45.0%
!! GGTGGGATTATTGTTGACTC+TGG - chr2_1:2580881-2580900 Msa0187820:CDS 45.0%
TTGACTCTGTAACCGCGTTA+TGG + chr2_1:2580912-2580931 None:intergenic 45.0%
AAGTGTTAGGGTTCCAACTG+TGG - chr2_1:2581036-2581055 Msa0187820:CDS 45.0%
!! GGTGGAGATCGGTTTTCTTG+AGG + chr2_1:2580161-2580180 None:intergenic 50.0%
CAAGAAAACCGATCTCCACC+CGG - chr2_1:2580161-2580180 Msa0187820:CDS 50.0%
! GTTGAAGCTCGGTTTGAACC+GGG + chr2_1:2580182-2580201 None:intergenic 50.0%
GGCGAGTATTTCTCCAGACT+AGG - chr2_1:2580249-2580268 Msa0187820:CDS 50.0%
!! TGGTTGGCCTATACCTAGTC+TGG + chr2_1:2580265-2580284 None:intergenic 50.0%
!! CACGGGTTGATAGGTTGATG+AGG + chr2_1:2580397-2580416 None:intergenic 50.0%
CTCATCAACCTATCAACCCG+TGG - chr2_1:2580395-2580414 Msa0187820:intron 50.0%
TTGTTCTGCTTCACAGGCCA+CGG + chr2_1:2580415-2580434 None:intergenic 50.0%
! GGTGATGGCTCATTCACTGT+AGG - chr2_1:2580495-2580514 Msa0187820:CDS 50.0%
! AACCGTGTTGCTATCGGTTG+TGG - chr2_1:2580564-2580583 Msa0187820:CDS 50.0%
!! GGTGCAAGTACTGAATCACC+GGG + chr2_1:2580749-2580768 None:intergenic 50.0%
!! CGGTGCAAGTACTGAATCAC+CGG + chr2_1:2580750-2580769 None:intergenic 50.0%
ACTCTGTAACCGCGTTATGG+CGG + chr2_1:2580909-2580928 None:intergenic 50.0%
! TTGATTCCGGTGGATTCTGC+CGG - chr2_1:2581109-2581128 Msa0187820:CDS 50.0%
AACTTTCCGGCAGAATCCAC+CGG + chr2_1:2581118-2581137 None:intergenic 50.0%
!! GGTGGAGATCGGTTTTCTTG+AGG + chr2_1:2580161-2580180 None:intergenic 50.0%
CAAGAAAACCGATCTCCACC+CGG - chr2_1:2580161-2580180 Msa0187820:CDS 50.0%
! GTTGAAGCTCGGTTTGAACC+GGG + chr2_1:2580182-2580201 None:intergenic 50.0%
GGCGAGTATTTCTCCAGACT+AGG - chr2_1:2580249-2580268 Msa0187820:CDS 50.0%
!! TGGTTGGCCTATACCTAGTC+TGG + chr2_1:2580265-2580284 None:intergenic 50.0%
!! CACGGGTTGATAGGTTGATG+AGG + chr2_1:2580397-2580416 None:intergenic 50.0%
CTCATCAACCTATCAACCCG+TGG - chr2_1:2580395-2580414 Msa0187820:intron 50.0%
TTGTTCTGCTTCACAGGCCA+CGG + chr2_1:2580415-2580434 None:intergenic 50.0%
! GGTGATGGCTCATTCACTGT+AGG - chr2_1:2580495-2580514 Msa0187820:CDS 50.0%
! AACCGTGTTGCTATCGGTTG+TGG - chr2_1:2580564-2580583 Msa0187820:CDS 50.0%
!! GGTGCAAGTACTGAATCACC+GGG + chr2_1:2580749-2580768 None:intergenic 50.0%
!! CGGTGCAAGTACTGAATCAC+CGG + chr2_1:2580750-2580769 None:intergenic 50.0%
ACTCTGTAACCGCGTTATGG+CGG + chr2_1:2580909-2580928 None:intergenic 50.0%
! TTGATTCCGGTGGATTCTGC+CGG - chr2_1:2581109-2581128 Msa0187820:CDS 50.0%
AACTTTCCGGCAGAATCCAC+CGG + chr2_1:2581118-2581137 None:intergenic 50.0%
GGTTTGAACCGGGTGGAGAT+CGG + chr2_1:2580172-2580191 None:intergenic 55.0%
CTCTCTACACCAGTCTCCTC+CGG - chr2_1:2580210-2580229 Msa0187820:CDS 55.0%
!! TTCACAGGCCACGGGTTGAT+AGG + chr2_1:2580406-2580425 None:intergenic 55.0%
TGTTCTGCTTCACAGGCCAC+GGG + chr2_1:2580414-2580433 None:intergenic 55.0%
AGCAACACGGTTCACTGACC+CGG + chr2_1:2580556-2580575 None:intergenic 55.0%
GGCCACAACCGATAGCAACA+CGG + chr2_1:2580569-2580588 None:intergenic 55.0%
! CGGTTGTGGCCATGATAACG+AGG - chr2_1:2580578-2580597 Msa0187820:CDS 55.0%
GGTTTATTCGTCGGTGCAGC+TGG - chr2_1:2580599-2580618 Msa0187820:CDS 55.0%
! GTCGGTGCAGCTGGTTTACT+CGG - chr2_1:2580608-2580627 Msa0187820:CDS 55.0%
! GCAGCTGGTTTACTCGGACT+CGG - chr2_1:2580614-2580633 Msa0187820:CDS 55.0%
!! GTTTACTCGGACTCGGTGGT+GGG - chr2_1:2580621-2580640 Msa0187820:CDS 55.0%
! TGGGCAGATGGTTGCTGTTC+CGG - chr2_1:2580832-2580851 Msa0187820:CDS 55.0%
!! GCGATGGTTCTGGAAGTGGT+GGG - chr2_1:2580864-2580883 Msa0187820:CDS 55.0%
!! ACTCTGGAACCGCCATAACG+CGG - chr2_1:2580897-2580916 Msa0187820:CDS 55.0%
GGTTTGAACCGGGTGGAGAT+CGG + chr2_1:2580172-2580191 None:intergenic 55.0%
CTCTCTACACCAGTCTCCTC+CGG - chr2_1:2580210-2580229 Msa0187820:CDS 55.0%
!! TTCACAGGCCACGGGTTGAT+AGG + chr2_1:2580406-2580425 None:intergenic 55.0%
TGTTCTGCTTCACAGGCCAC+GGG + chr2_1:2580414-2580433 None:intergenic 55.0%
AGCAACACGGTTCACTGACC+CGG + chr2_1:2580556-2580575 None:intergenic 55.0%
GGCCACAACCGATAGCAACA+CGG + chr2_1:2580569-2580588 None:intergenic 55.0%
! CGGTTGTGGCCATGATAACG+AGG - chr2_1:2580578-2580597 Msa0187820:CDS 55.0%
GGTTTATTCGTCGGTGCAGC+TGG - chr2_1:2580599-2580618 Msa0187820:CDS 55.0%
! GTCGGTGCAGCTGGTTTACT+CGG - chr2_1:2580608-2580627 Msa0187820:CDS 55.0%
! GCAGCTGGTTTACTCGGACT+CGG - chr2_1:2580614-2580633 Msa0187820:CDS 55.0%
!! GTTTACTCGGACTCGGTGGT+GGG - chr2_1:2580621-2580640 Msa0187820:CDS 55.0%
! TGGGCAGATGGTTGCTGTTC+CGG - chr2_1:2580832-2580851 Msa0187820:CDS 55.0%
!! GCGATGGTTCTGGAAGTGGT+GGG - chr2_1:2580864-2580883 Msa0187820:CDS 55.0%
!! ACTCTGGAACCGCCATAACG+CGG - chr2_1:2580897-2580916 Msa0187820:CDS 55.0%
! GAAGCTCGGTTTGAACCGGG+TGG + chr2_1:2580179-2580198 None:intergenic 60.0%
ACACCAGTCTCCTCCGGTTC+TGG - chr2_1:2580216-2580235 Msa0187820:CDS 60.0%
GGAGAAATACTCGCCGGAGC+CGG + chr2_1:2580244-2580263 None:intergenic 60.0%
! GCTGGTTTACTCGGACTCGG+TGG - chr2_1:2580617-2580636 Msa0187820:CDS 60.0%
!! GGTTTACTCGGACTCGGTGG+TGG - chr2_1:2580620-2580639 Msa0187820:CDS 60.0%
! GCAGATGGTTGCTGTTCCGG+CGG - chr2_1:2580835-2580854 Msa0187820:CDS 60.0%
!! GGCGATGGTTCTGGAAGTGG+TGG - chr2_1:2580863-2580882 Msa0187820:CDS 60.0%
! GAAGCTCGGTTTGAACCGGG+TGG + chr2_1:2580179-2580198 None:intergenic 60.0%
ACACCAGTCTCCTCCGGTTC+TGG - chr2_1:2580216-2580235 Msa0187820:CDS 60.0%
GGAGAAATACTCGCCGGAGC+CGG + chr2_1:2580244-2580263 None:intergenic 60.0%
! GCTGGTTTACTCGGACTCGG+TGG - chr2_1:2580617-2580636 Msa0187820:CDS 60.0%
!! GGTTTACTCGGACTCGGTGG+TGG - chr2_1:2580620-2580639 Msa0187820:CDS 60.0%
! GCAGATGGTTGCTGTTCCGG+CGG - chr2_1:2580835-2580854 Msa0187820:CDS 60.0%
!! GGCGATGGTTCTGGAAGTGG+TGG - chr2_1:2580863-2580882 Msa0187820:CDS 60.0%
GAGCCAGAACCGGAGGAGAC+TGG + chr2_1:2580222-2580241 None:intergenic 65.0%
!! GTCTCCTCCGGTTCTGGCTC+CGG - chr2_1:2580222-2580241 Msa0187820:CDS 65.0%
! GATGGTTGCTGTTCCGGCGG+CGG - chr2_1:2580838-2580857 Msa0187820:CDS 65.0%
!! GCGGGCGATGGTTCTGGAAG+TGG - chr2_1:2580860-2580879 Msa0187820:CDS 65.0%
GAGCCAGAACCGGAGGAGAC+TGG + chr2_1:2580222-2580241 None:intergenic 65.0%
!! GTCTCCTCCGGTTCTGGCTC+CGG - chr2_1:2580222-2580241 Msa0187820:CDS 65.0%
! GATGGTTGCTGTTCCGGCGG+CGG - chr2_1:2580838-2580857 Msa0187820:CDS 65.0%
!! GCGGGCGATGGTTCTGGAAG+TGG - chr2_1:2580860-2580879 Msa0187820:CDS 65.0%
!! TCCGGTTCTGGCTCCGGCTC+CGG - chr2_1:2580228-2580247 Msa0187820:CDS 70.0%
!! TCCGGTTCTGGCTCCGGCTC+CGG - chr2_1:2580228-2580247 Msa0187820:CDS 70.0%
GGAGCCGGAGCCAGAACCGG+AGG + chr2_1:2580229-2580248 None:intergenic 75.0%
GCCGGAGCCGGAGCCAGAAC+CGG + chr2_1:2580232-2580251 None:intergenic 75.0%
! GGTTGCTGTTCCGGCGGCGG+CGG - chr2_1:2580841-2580860 Msa0187820:CDS 75.0%
! GTTGCTGTTCCGGCGGCGGC+GGG - chr2_1:2580842-2580861 Msa0187820:CDS 75.0%
GGAGCCGGAGCCAGAACCGG+AGG + chr2_1:2580229-2580248 None:intergenic 75.0%
GCCGGAGCCGGAGCCAGAAC+CGG + chr2_1:2580232-2580251 None:intergenic 75.0%
! GGTTGCTGTTCCGGCGGCGG+CGG - chr2_1:2580841-2580860 Msa0187820:CDS 75.0%
! GTTGCTGTTCCGGCGGCGGC+GGG - chr2_1:2580842-2580861 Msa0187820:CDS 75.0%
! GAACCATCGCCCGCCGCCGC+CGG + chr2_1:2580854-2580873 None:intergenic 80.0%
! GCGGCGGCGGGCGATGGTTC+TGG - chr2_1:2580854-2580873 Msa0187820:CDS 80.0%
! GAACCATCGCCCGCCGCCGC+CGG + chr2_1:2580854-2580873 None:intergenic 80.0%
! GCGGCGGCGGGCGATGGTTC+TGG - chr2_1:2580854-2580873 Msa0187820:CDS 80.0%
! GTTCCGGCGGCGGCGGGCGA+TGG - chr2_1:2580848-2580867 Msa0187820:CDS 85.0%
! GTTCCGGCGGCGGCGGGCGA+TGG - chr2_1:2580848-2580867 Msa0187820:CDS 85.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 2579784 2581261 2579784 ID=Msa0187820;Name=Msa0187820
chr2_1 mRNA 2579784 2581261 2579784 ID=Msa0187820-mRNA-1;Parent=Msa0187820;Name=Msa0187820-mRNA-1;_AED=0.02;_eAED=0.02;_QI=0|0|0|1|1|1|2|0|470
chr2_1 exon 2580446 2581261 2580446 ID=Msa0187820-mRNA-1:exon:1191;Parent=Msa0187820-mRNA-1
chr2_1 exon 2579784 2580380 2579784 ID=Msa0187820-mRNA-1:exon:1190;Parent=Msa0187820-mRNA-1
chr2_1 CDS 2580446 2581261 2580446 ID=Msa0187820-mRNA-1:cds;Parent=Msa0187820-mRNA-1
chr2_1 CDS 2579784 2580380 2579784 ID=Msa0187820-mRNA-1:cds;Parent=Msa0187820-mRNA-1
Gene Sequence

>Msa0187820

ATGGCTTCCAAAAAAGCACTTATTTCCTTCAACTTATCACTTCTCATTACAACAATCTATCTTCTTCCATCTCCAACTTCCTCATCTAGAATCATTACTCCTTCACACACTTCCCTTCTTGATATCTCTTCCTCAATTCACCAAACCAAAAAAGTCCTTAATTTTAACCCTAATAACAATAATCTTCTTGAAGAAACCCTAAACGAAAAATCCATTTTTTCAAACTCTTCATCTTTCTCAATTCTCATACATCCAAGAGAAAATCTCATCAATGAAAACCACAAAGACTATACCAAACTTATGCAAGCTAGACTCACTCGTGACTCAGCTCGAGTTGACTCACTCACTACAAAACTCATACTTTCTCTAAACAACCTCAAGAAAACCGATCTCCACCCGGTTCAAACCGAGCTTCAACAAGAAGATCTCTCTACACCAGTCTCCTCCGGTTCTGGCTCCGGCTCCGGCGAGTATTTCTCCAGACTAGGTATAGGCCAACCACCAAAACCCTACTACATGGTTCTTGACACAGGTAGTGACGTAAACTGGATCCAATGCAAACCATGCACAGAATGTTACCAACAAACTGACCCAATATACGACCCAACTTCCTCATCAACCTATCAACCCGTGGCCTGTGAAGCAGAACAATGTCAATCACTCGAACTTTCTGGTTGTCGTGATGGTAAATGTCAATACCAAGTTTCTTACGGTGATGGCTCATTCACTGTAGGAGAATATAGCACTGAAACAGTCTCTTTTGGAAACTCCGGGTCAGTGAACCGTGTTGCTATCGGTTGTGGCCATGATAACGAGATCAAAGCTTCATCGTTTTCTTACTGTTTAGTTGATCGTAACTCGGGAAAATCGTCAACTCTTGAGTTTAACTCGGTTCAACCCGGTGATTCAGTACTTGCACCGATGCTCAAGAATCAGAGAGTTAATACGTTTTATTTTGTTCAACTCACTGGTATAAGTGTTGGTGGGCAGATGGTTGCTGTTCCGGCGGCGGCGGGCGATGGTTCTGGAAGTGGTGGGATTATTGTTGACTCTGGAACCGCCATAACGCGGTTACAGAGTCAACTTTATAACTCAGTTCGCGACGAGTTTAAAAGAATGACTCAGAATTTACGTTCTGCTGAGAATTTTGCATTGTTTGATACTTGTTATGATTTGTCAAATTTGCAAAGTGTTAGGGTTCCAACTGTGGCTTTTCATTTTGGTGAGAATAAGGTTTGGAGTTTGCCGGCGAAGAATTACTTGATTCCGGTGGATTCTGCCGGAAAGTTTTGCTTTGCTTTTGCTCCGACGAGTTCTTCATTGTCTATTATTGGAAATGTTCAACAACAAGGGACTCGTGTTACTTTTAATCTTGCTAATAATTTAGTAGGATTCTCAAATGATAAGTGCTAG
Protein sequence

>Msa0187820

MASKKALISFNLSLLITTIYLLPSPTSSSRIITPSHTSLLDISSSIHQTKKVLNFNPNNNNLLEETLNEKSIFSNSSSFSILIHPRENLINENHKDYTKLMQARLTRDSARVDSLTTKLILSLNNLKKTDLHPVQTELQQEDLSTPVSSGSGSGSGEYFSRLGIGQPPKPYYMVLDTGSDVNWIQCKPCTECYQQTDPIYDPTSSSTYQPVACEAEQCQSLELSGCRDGKCQYQVSYGDGSFTVGEYSTETVSFGNSGSVNRVAIGCGHDNEIKASSFSYCLVDRNSGKSSTLEFNSVQPGDSVLAPMLKNQRVNTFYFVQLTGISVGGQMVAVPAAAGDGSGSGGIIVDSGTAITRLQSQLYNSVRDEFKRMTQNLRSAENFALFDTCYDLSNLQSVRVPTVAFHFGENKVWSLPAKNYLIPVDSAGKFCFAFAPTSSSLSIIGNVQQQGTRVTFNLANNLVGFSNDKC*