AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene64209


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene64209.t1 MTR_1g024840 96.835 158 4 1 1 158 1 157 4.69e-110 310
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene64209.t1 AT1G75690 75.497 151 34 3 8 158 7 154 2.51e-78 229

Find 33 sgRNAs with CRISPR-Local

Find 93 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGCTGCGTGTTATCTCTTT+TGG 0.263030 1.3:-1231481 None:intergenic
TGCTTCCCATGCAATGGTTC+TGG 0.280691 1.3:+1231504 MS.gene64209:CDS
CGTGGATAAAATGTGGGTTT+TGG 0.374056 1.3:-1231143 None:intergenic
TTGAGTGTGGCTGTTGGAAT+TGG 0.393770 1.3:+1231313 MS.gene64209:CDS
AGAACCATTGCATGGGAAGC+AGG 0.424768 1.3:-1231502 None:intergenic
AAGTTCAACAGTCACATTAC+CGG 0.426738 1.3:-1232473 None:intergenic
CAGGTAGTTGCTATAACTGT+TGG 0.428401 1.3:+1231286 MS.gene64209:intron
TTTAAGAGGAGAAGAAAGAA+AGG 0.455802 1.3:-1231078 None:intergenic
GTAGTAGATAAGGATTTAAG+AGG 0.469567 1.3:-1231092 None:intergenic
TGTACAACTTGTCAAGGATC+TGG 0.478203 1.3:+1232556 MS.gene64209:CDS
TTTAATACGTGGATAAAATG+TGG 0.513316 1.3:-1231150 None:intergenic
AGAAAGGTGGAATGTAATTG+AGG 0.514748 1.3:-1231062 None:intergenic
TAATTGTGTAGTAGTAGATA+AGG 0.518803 1.3:-1231102 None:intergenic
TTAATACGTGGATAAAATGT+GGG 0.525605 1.3:-1231149 None:intergenic
AAGAGGAGAAGAAAGAAAGG+TGG 0.539027 1.3:-1231075 None:intergenic
CAGCCCTGCTTCCCATGCAA+TGG 0.546625 1.3:+1231498 MS.gene64209:CDS
GAACACCGTACCTACGATCA+AGG 0.554130 1.3:-1232594 None:intergenic
GGTAATGTGACTGTTGAACT+TGG 0.571939 1.3:+1232475 MS.gene64209:CDS
GAACCATTGCATGGGAAGCA+GGG 0.571952 1.3:-1231501 None:intergenic
TGAACTTGGTGGAGCTGAGA+CGG 0.581832 1.3:+1232489 MS.gene64209:CDS
CTGCATAAATTGTGATGGTG+CGG 0.586364 1.3:+1232522 MS.gene64209:CDS
TGCATAAATTGTGATGGTGC+GGG 0.591712 1.3:+1232523 MS.gene64209:CDS
CGAGCTCCAGAACCATTGCA+TGG 0.592093 1.3:-1231510 None:intergenic
TCTCGCTGCATAAATTGTGA+TGG 0.600927 1.3:+1232517 MS.gene64209:CDS
CTGACATGTACAACTTGTCA+AGG 0.612986 1.3:+1232550 MS.gene64209:CDS
ATTTGCGATTCATAAAACAC+TGG 0.613193 1.3:-1231340 None:intergenic
ACTTGGTGGAGCTGAGACGG+AGG 0.632275 1.3:+1232492 MS.gene64209:CDS
AGTTCAAGAGCTTTAATACG+TGG 0.639758 1.3:-1231161 None:intergenic
AACCTCGTTACCTTGATCGT+AGG 0.640316 1.3:+1232584 MS.gene64209:CDS
GAGCTCCAGAACCATTGCAT+GGG 0.645320 1.3:-1231509 None:intergenic
AATGTGACTGTTGAACTTGG+TGG 0.646977 1.3:+1232478 MS.gene64209:CDS
TCTTGAACTTGATCAAAACA+CGG 0.674004 1.3:+1231175 MS.gene64209:CDS
TACCTACGATCAAGGTAACG+AGG 0.728545 1.3:-1232586 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGATATAGTAATAAATAAA+TGG - chr1.3:1231390-1231409 None:intergenic 10.0%
!! ATTATTAAATTATTACAAAG+GGG - chr1.3:1232631-1232650 None:intergenic 10.0%
!! CATTATTAAATTATTACAAA+GGG - chr1.3:1232632-1232651 None:intergenic 10.0%
!! TCATTATTAAATTATTACAA+AGG - chr1.3:1232633-1232652 None:intergenic 10.0%
!!! TAAAATACTGATTAATAATA+TGG - chr1.3:1232216-1232235 None:intergenic 10.0%
!! AAGTTAAATTATATAACTTC+TGG + chr1.3:1231986-1232005 MS.gene64209:intron 15.0%
!! TATACCAAGAATAATAAAAT+AGG + chr1.3:1232258-1232277 MS.gene64209:intron 15.0%
!! TATATCATATAATAATGTGT+TGG + chr1.3:1231402-1231421 MS.gene64209:intron 15.0%
!!! ATAACCTATTTTATTATTCT+TGG - chr1.3:1232265-1232284 None:intergenic 15.0%
!!! TGGATAAGTTTTTTTTTTAT+AGG + chr1.3:1231802-1231821 MS.gene64209:intron 15.0%
!! ATTTGTAAAAGATTGCTTAT+AGG + chr1.3:1231949-1231968 MS.gene64209:intron 20.0%
!! CAAAAAGTTTATATGCTTAT+AGG + chr1.3:1231770-1231789 MS.gene64209:intron 20.0%
!! CTGCAACAAATTAAAAAAAA+AGG - chr1.3:1231460-1231479 None:intergenic 20.0%
!! TGAATGAATGAATAAGAAAT+AGG - chr1.3:1231217-1231236 None:intergenic 20.0%
!!! AACATTTTCAAAGATAAGAT+TGG - chr1.3:1232416-1232435 None:intergenic 20.0%
!!! ACACTTTATACTTTTATGAA+TGG + chr1.3:1231567-1231586 MS.gene64209:intron 20.0%
!!! AGTTTTTTTTTTATAGGTAG+TGG + chr1.3:1231808-1231827 MS.gene64209:intron 20.0%
!!! CTATAAGCATATAAACTTTT+TGG - chr1.3:1231772-1231791 None:intergenic 20.0%
!!! CTTTTTTTTTAATTTGTTGC+AGG + chr1.3:1231458-1231477 MS.gene64209:intron 20.0%
! ATGAATGAATAAGAAATAGG+AGG - chr1.3:1231214-1231233 None:intergenic 25.0%
! CTCACAAGTTATTGTTAATT+TGG + chr1.3:1231609-1231628 MS.gene64209:intron 25.0%
! TAATTGTGTAGTAGTAGATA+AGG - chr1.3:1231105-1231124 None:intergenic 25.0%
! TTAATACGTGGATAAAATGT+GGG - chr1.3:1231152-1231171 None:intergenic 25.0%
! TTTAATACGTGGATAAAATG+TGG - chr1.3:1231153-1231172 None:intergenic 25.0%
!! AATAGTGTTAGCAATTTTCA+TGG - chr1.3:1232716-1232735 None:intergenic 25.0%
!! CTTTATACTTTTATGAATGG+AGG + chr1.3:1231570-1231589 MS.gene64209:intron 25.0%
!! TACCATGATAATTTGTCATT+TGG + chr1.3:1232673-1232692 MS.gene64209:intron 25.0%
!!! TGAATTAGCTTTTAGTTCAT+TGG + chr1.3:1231706-1231725 MS.gene64209:intron 25.0%
AAACAAAATCTGCATTTCTC+TGG - chr1.3:1232448-1232467 None:intergenic 30.0%
AAGAAATTAGCAACCTGATA+TGG - chr1.3:1232391-1232410 None:intergenic 30.0%
ATTTGCGATTCATAAAACAC+TGG - chr1.3:1231343-1231362 None:intergenic 30.0%
GTAGTAGATAAGGATTTAAG+AGG - chr1.3:1231095-1231114 None:intergenic 30.0%
TCTTGAACTTGATCAAAACA+CGG + chr1.3:1231175-1231194 MS.gene64209:CDS 30.0%
TTGCTTATAGGTAGTGTTTA+TGG + chr1.3:1231961-1231980 MS.gene64209:intron 30.0%
TTTAAGAGGAGAAGAAAGAA+AGG - chr1.3:1231081-1231100 None:intergenic 30.0%
! AGAGAAATGCAGATTTTGTT+TGG + chr1.3:1232447-1232466 MS.gene64209:intron 30.0%
!! GAGAAATGCAGATTTTGTTT+GGG + chr1.3:1232448-1232467 MS.gene64209:intron 30.0%
!!! ATGTGAGTGTTTGTTTTTTC+AGG + chr1.3:1231267-1231286 MS.gene64209:intron 30.0%
AAGTTCAACAGTCACATTAC+CGG - chr1.3:1232476-1232495 None:intergenic 35.0%
AATCAGTTCAAGTCCATATC+AGG + chr1.3:1232375-1232394 MS.gene64209:intron 35.0%
AGAAAGGTGGAATGTAATTG+AGG - chr1.3:1231065-1231084 None:intergenic 35.0%
ATGTTGTAGTGTAATGTGCA+AGG + chr1.3:1231675-1231694 MS.gene64209:intron 35.0%
CGCCAAATGACAAATTATCA+TGG - chr1.3:1232678-1232697 None:intergenic 35.0%
CTAAAAGCTAATTCAAACGC+TGG - chr1.3:1231701-1231720 None:intergenic 35.0%
! AGTTCAAGAGCTTTAATACG+TGG - chr1.3:1231164-1231183 None:intergenic 35.0%
! ATGCTAGCTTGAACAATCAT+TGG - chr1.3:1231931-1231950 None:intergenic 35.0%
!! TTATGAATGGAGGAGCATTA+TGG + chr1.3:1231580-1231599 MS.gene64209:intron 35.0%
!! TTGTTGAGCTCTAAGTCAAA+GGG + chr1.3:1232122-1232141 MS.gene64209:intron 35.0%
!! TTTGTTGAGCTCTAAGTCAA+AGG + chr1.3:1232121-1232140 MS.gene64209:intron 35.0%
AAGAGGAGAAGAAAGAAAGG+TGG - chr1.3:1231078-1231097 None:intergenic 40.0%
AATGTGACTGTTGAACTTGG+TGG + chr1.3:1232478-1232497 MS.gene64209:CDS 40.0%
ACAACCTAGTGTCTAGTAAC+CGG - chr1.3:1232349-1232368 None:intergenic 40.0%
ATGCTTATAGGCAGTGTTTG+TGG + chr1.3:1231782-1231801 MS.gene64209:intron 40.0%
CAGGTAGTTGCTATAACTGT+TGG + chr1.3:1231286-1231305 MS.gene64209:intron 40.0%
CTGACATGTACAACTTGTCA+AGG + chr1.3:1232550-1232569 MS.gene64209:CDS 40.0%
GACTCTGGTAATATGGAGTT+CGG + chr1.3:1231876-1231895 MS.gene64209:intron 40.0%
GCGATGCTTATGATTGTGTT+TGG + chr1.3:1232313-1232332 MS.gene64209:intron 40.0%
GGAAAGAGACTCTGGTAATA+TGG + chr1.3:1231869-1231888 MS.gene64209:intron 40.0%
GGTAATGTGACTGTTGAACT+TGG + chr1.3:1232475-1232494 MS.gene64209:CDS 40.0%
TCTCGCTGCATAAATTGTGA+TGG + chr1.3:1232517-1232536 MS.gene64209:CDS 40.0%
TGGTAATATGGAGTTCGGTT+GGG + chr1.3:1231881-1231900 MS.gene64209:intron 40.0%
TGTACAACTTGTCAAGGATC+TGG + chr1.3:1232556-1232575 MS.gene64209:CDS 40.0%
! CGTGGATAAAATGTGGGTTT+TGG - chr1.3:1231146-1231165 None:intergenic 40.0%
! CTGCATAAATTGTGATGGTG+CGG + chr1.3:1232522-1232541 MS.gene64209:CDS 40.0%
! TGCATAAATTGTGATGGTGC+GGG + chr1.3:1232523-1232542 MS.gene64209:CDS 40.0%
!! AACTGTTGGTGTTTTGAGTG+TGG + chr1.3:1231300-1231319 MS.gene64209:CDS 40.0%
!! TGCAGATTTTGTTTGGGAAC+CGG + chr1.3:1232454-1232473 MS.gene64209:CDS 40.0%
AACCTCGTTACCTTGATCGT+AGG + chr1.3:1232584-1232603 MS.gene64209:CDS 45.0%
CGTTACCTTGATCGTAGGTA+CGG + chr1.3:1232589-1232608 MS.gene64209:intron 45.0%
CTGGTAATATGGAGTTCGGT+TGG + chr1.3:1231880-1231899 MS.gene64209:intron 45.0%
CTTAACCGTCATGTTCTCGA+GGG + chr1.3:1231848-1231867 MS.gene64209:intron 45.0%
TACCTACGATCAAGGTAACG+AGG - chr1.3:1232589-1232608 None:intergenic 45.0%
TCTTAACCGTCATGTTCTCG+AGG + chr1.3:1231847-1231866 MS.gene64209:intron 45.0%
TCTTTCCCTCGAGAACATGA+CGG - chr1.3:1231856-1231875 None:intergenic 45.0%
TTGAGTGTGGCTGTTGGAAT+TGG + chr1.3:1231313-1231332 MS.gene64209:CDS 45.0%
! TGTGTTTGGTTGCTTTGTGC+CGG + chr1.3:1232327-1232346 MS.gene64209:intron 45.0%
!! TGAATGGAGGAGCATTATGG+AGG + chr1.3:1231583-1231602 MS.gene64209:intron 45.0%
!!! TTATTATTTTAACTTTTTAT+CGG + chr1.3:1232058-1232077 MS.gene64209:intron 5.0%
AGAACCATTGCATGGGAAGC+AGG - chr1.3:1231505-1231524 None:intergenic 50.0%
GAACACCGTACCTACGATCA+AGG - chr1.3:1232597-1232616 None:intergenic 50.0%
GAACCATTGCATGGGAAGCA+GGG - chr1.3:1231504-1231523 None:intergenic 50.0%
GAGCTCCAGAACCATTGCAT+GGG - chr1.3:1231512-1231531 None:intergenic 50.0%
TGCTTCCCATGCAATGGTTC+TGG + chr1.3:1231504-1231523 MS.gene64209:CDS 50.0%
TTCTCGAGGGAAAGAGACTC+TGG + chr1.3:1231861-1231880 MS.gene64209:intron 50.0%
! CTAAGTCAAAGGGAGTGTGG+AGG + chr1.3:1232132-1232151 MS.gene64209:intron 50.0%
! GCTCTAAGTCAAAGGGAGTG+TGG + chr1.3:1232129-1232148 MS.gene64209:intron 50.0%
! GGGCTGCGTGTTATCTCTTT+TGG - chr1.3:1231484-1231503 None:intergenic 50.0%
! TGAACTTGGTGGAGCTGAGA+CGG + chr1.3:1232489-1232508 MS.gene64209:CDS 50.0%
!! GGTGTTTTGAGTGTGGCTGT+TGG + chr1.3:1231307-1231326 MS.gene64209:CDS 50.0%
!! TGTGCCGGTTACTAGACACT+AGG + chr1.3:1232342-1232361 MS.gene64209:intron 50.0%
CGAGCTCCAGAACCATTGCA+TGG - chr1.3:1231513-1231532 None:intergenic 55.0%
CAGCCCTGCTTCCCATGCAA+TGG + chr1.3:1231498-1231517 MS.gene64209:CDS 60.0%
! ACTTGGTGGAGCTGAGACGG+AGG + chr1.3:1232492-1232511 MS.gene64209:CDS 60.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 1231035 1232778 1231035 ID=MS.gene64209
chr1.3 mRNA 1231035 1232778 1231035 ID=MS.gene64209.t1;Parent=MS.gene64209
chr1.3 exon 1231035 1231196 1231035 ID=MS.gene64209.t1.exon1;Parent=MS.gene64209.t1
chr1.3 CDS 1231035 1231196 1231035 ID=cds.MS.gene64209.t1;Parent=MS.gene64209.t1
chr1.3 exon 1231289 1231372 1231289 ID=MS.gene64209.t1.exon2;Parent=MS.gene64209.t1
chr1.3 CDS 1231289 1231372 1231289 ID=cds.MS.gene64209.t1;Parent=MS.gene64209.t1
chr1.3 exon 1231480 1231531 1231480 ID=MS.gene64209.t1.exon3;Parent=MS.gene64209.t1
chr1.3 CDS 1231480 1231531 1231480 ID=cds.MS.gene64209.t1;Parent=MS.gene64209.t1
chr1.3 exon 1232449 1232605 1232449 ID=MS.gene64209.t1.exon4;Parent=MS.gene64209.t1
chr1.3 CDS 1232449 1232605 1232449 ID=cds.MS.gene64209.t1;Parent=MS.gene64209.t1
chr1.3 exon 1232757 1232778 1232757 ID=MS.gene64209.t1.exon5;Parent=MS.gene64209.t1
chr1.3 CDS 1232757 1232778 1232757 ID=cds.MS.gene64209.t1;Parent=MS.gene64209.t1
Gene Sequence

>MS.gene64209

ATGACAATATCTTATTCACTTTCACTTCCTCAATTACATTCCACCTTTCTTTCTTCTCCTCTTAAATCCTTATCTACTACTACACAATTAGCTAGAAATAAATTTCAACCAAAACCCACATTTTATCCACGTATTAAAGCTCTTGAACTTGATCAAAACACGGTTGTTTATAATTTCCTCCTATTTCTTATTCATTCATTCAATGTTTTTTTGTTACATATTTATCTTAAATATGTGAGTGTTTGTTTTTTCAGGTAGTTGCTATAACTGTTGGTGTTTTGAGTGTGGCTGTTGGAATTGGCATACCAGTGTTTTATGAATCGCAAATTGATAATGCTGTAAAGTGTTTTTACCATTTATTTATTACTATATCATATAATAATGTGTTGGATTAAAATTTAGTTATATATGATATATACGTACCTTTTTTTTTAATTTGTTGCAGGCCAAAAGAGATAACACGCAGCCCTGCTTCCCATGCAATGGTTCTGGAGCTCGTAAGTATCATTTTTTTCTCTTCAATTTCATCATCACACTTTATACTTTTATGAATGGAGGAGCATTATGGAGGTAGCTCACAAGTTATTGTTAATTTGGAGTAGTTGTTAGTACATAACTAACTACTTATATAATATTGTTGATGTTGTAGTGTAATGTGCAAGGCCAGCGTTTGAATTAGCTTTTAGTTCATTGGAATTTCTCGATTAAAATATGTCTATGATTCTTGACATACACCAAAAAGTTTATATGCTTATAGGCAGTGTTTGTGGATAAGTTTTTTTTTTATAGGTAGTGGTATTTCTTGTCTTAACTCTTAACCGTCATGTTCTCGAGGGAAAGAGACTCTGGTAATATGGAGTTCGGTTGGGAGTTAACTGTCAAGTAAAGTCTGACCAATGATTGTTCAAGCTAGCATTTGTAAAAGATTGCTTATAGGTAGTGTTTATGGATAAGTTAAATTATATAACTTCTGGAAGAAAAATAATTTAAAATGCAGAATTTCATTCTAAATTAGACAAGAAGTTATTATTTTAACTTTTTATCGGTATTGTACGATTCTTTTTATAATTCTTTTTTTTAGAATTGTTTGTTGAGCTCTAAGTCAAAGGGAGTGTGGAGGTAGTACGTATTTGCTTGCAAAGAAAATTATATTAATTGCTTCTCACTAACACTGTTTTCCATATTATTAATCAGTATTTTATTAAATCTGATTCATTTCTATCTATACCAAGAATAATAAAATAGGTTATTGCAATTTTGTTTTTATTGAAACTTTTTGCGATGCTTATGATTGTGTTTGGTTGCTTTGTGCCGGTTACTAGACACTAGGTTGTGAATGTAATCAGTTCAAGTCCATATCAGGTTGCTAATTTCTTTACCAATCTTATCTTTGAAAATGTTACACTTTTGCCAGAGAAATGCAGATTTTGTTTGGGAACCGGTAATGTGACTGTTGAACTTGGTGGAGCTGAGACGGAGGTCTCTCGCTGCATAAATTGTGATGGTGCGGGTTCACTGACATGTACAACTTGTCAAGGATCTGGAATTCAACCTCGTTACCTTGATCGTAGGTACGGTGTTCAATCTTATTTTCCCCTTTGTAATAATTTAATAATGAATAAGATTAGTCATTATGTATACCATGATAATTTGTCATTTGGCGCACGTTTTGCAATAACCATGAAAATTGCTAACACTATTTTGTGTTTAATATTTGATCAGAGAATTCAAAGATGATGACTGA

Protein sequence

>MS.gene64209.t1

MTISYSLSLPQLHSTFLSSPLKSLSTTTQLARNKFQPKPTFYPRIKALELDQNTVVAITVGVLSVAVGIGIPVFYESQIDNAAKRDNTQPCFPCNGSGAQKCRFCLGTGNVTVELGGAETEVSRCINCDGAGSLTCTTCQGSGIQPRYLDRREFKDDD