AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0547140

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0547140 MtrunA17_Chr4g0028971 84.397 423 4 3 1 361 276 698 0.0 703
Msa0547140 MtrunA17_Chr2g0326761 59.681 439 89 12 2 361 270 699 1.07e-163 474
Msa0547140 MtrunA17_Chr1g0181211 49.254 402 123 11 1 328 293 687 1.13e-108 332
Msa0547140 MtrunA17_Chr1g0199681 81.395 129 24 0 1 129 351 479 2.07e-69 230
Msa0547140 MtrunA17_Chr7g0264171 49.609 256 101 5 1 234 301 550 6.01e-69 227
Msa0547140 MtrunA17_Chr3g0115271 56.034 116 49 2 13 128 286 399 2.62e-36 139
Msa0547140 MtrunA17_Chr7g0264161 67.816 87 27 1 1 87 216 301 5.61e-35 130
Msa0547140 MtrunA17_Chr5g0424931 39.901 203 95 9 1 195 264 447 7.56e-30 120
Msa0547140 MtrunA17_Chr5g0436761 44.304 158 78 6 1 155 233 383 2.01e-27 112
Msa0547140 MtrunA17_Chr5g0417281 45.736 129 60 4 2 128 230 350 3.13e-23 100
Msa0547140 MtrunA17_Chr4g0070181 42.969 128 68 2 9 136 258 380 2.47e-22 99.0
Msa0547140 MtrunA17_Chr5g0425651 43.220 118 60 3 1 114 1 115 5.01e-22 90.1
Msa0547140 MtrunA17_Chr8g0376771 39.264 163 82 6 10 168 259 408 2.64e-21 95.5
Msa0547140 MtrunA17_Chr2g0301251 45.098 102 55 1 8 109 257 357 2.27e-20 92.8
Msa0547140 MtrunA17_Chr5g0433301 44.340 106 57 2 10 114 261 365 8.85e-20 90.9
Msa0547140 MtrunA17_Chr2g0285461 38.037 163 84 6 10 168 260 409 9.66e-20 90.9
Msa0547140 MtrunA17_Chr2g0277461 44.186 129 59 6 1 128 238 354 1.12e-19 90.1
Msa0547140 MtrunA17_Chr2g0282961 38.318 107 65 1 1 107 282 387 3.60e-19 89.0
Msa0547140 MtrunA17_Chr1g0178011 32.143 196 119 6 1 192 236 421 6.07e-19 87.8
Msa0547140 MtrunA17_Chr1g0156321 44.000 100 54 2 9 107 278 376 1.43e-18 87.4
Msa0547140 MtrunA17_Chr8g0389081 47.000 100 51 2 12 110 271 369 4.15e-18 86.3
Msa0547140 MtrunA17_Chr3g0109391 39.231 130 75 3 10 138 263 389 9.90e-18 84.7
Msa0547140 MtrunA17_Chr8g0385791 38.053 113 69 1 13 125 298 409 4.31e-17 82.8
Msa0547140 MtrunA17_Chr5g0425261 44.538 119 55 5 1 113 227 340 1.69e-16 80.1
Msa0547140 MtrunA17_Chr5g0424741 39.370 127 69 5 2 124 264 386 5.45e-16 79.3
Msa0547140 MtrunA17_Chr4g0009751 32.273 220 117 9 7 223 250 440 1.28e-15 78.2
Msa0547140 MtrunA17_Chr4g0046371 37.895 95 57 2 13 107 284 376 8.40e-15 75.9
Msa0547140 MtrunA17_Chr2g0326281 33.113 151 90 3 13 163 317 456 1.12e-14 75.5
Msa0547140 MtrunA17_Chr5g0424801 43.363 113 56 4 1 108 227 336 1.40e-14 74.3
Msa0547140 MtrunA17_Chr8g0346711 27.548 363 190 15 13 323 267 608 1.66e-14 74.7
Msa0547140 MtrunA17_Chr5g0417211 38.983 118 57 4 2 117 233 337 2.71e-13 70.5
Msa0547140 MtrunA17_Chr4g0029671 36.634 101 64 0 13 113 302 402 3.69e-13 70.9
Msa0547140 MtrunA17_Chr2g0276781 38.017 121 72 2 13 133 261 378 6.55e-13 70.1
Msa0547140 MtrunA17_Chr2g0277531 46.591 88 37 4 1 87 240 318 1.44e-12 67.8
Msa0547140 MtrunA17_Chr2g0289221 44.944 89 41 3 1 87 239 321 2.31e-12 67.4
Msa0547140 MtrunA17_Chr7g0239061 36.090 133 77 7 7 135 245 373 2.58e-12 68.2
Msa0547140 MtrunA17_Chr8g0356211 28.676 136 80 3 13 148 132 250 3.62e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0547140 AT2G28350.1 48.349 424 133 13 10 361 284 693 1.67e-113 345
Msa0547140 AT2G28350.2 46.851 397 125 13 10 334 284 666 1.50e-98 306
Msa0547140 AT4G30080.1 45.000 400 145 7 1 333 277 668 1.82e-95 298
Msa0547140 AT1G77850.1 50.000 130 60 4 2 130 273 398 5.95e-35 135
Msa0547140 AT1G77850.2 50.000 130 60 4 2 130 273 398 6.14e-35 135
Msa0547140 AT1G59750.4 29.526 359 182 12 13 327 260 591 1.49e-22 99.8
Msa0547140 AT1G59750.2 29.363 361 182 12 13 327 260 593 2.26e-22 99.0
Msa0547140 AT1G59750.1 28.809 361 187 12 13 327 260 596 4.64e-22 98.2
Msa0547140 AT1G59750.3 28.809 361 187 12 13 327 260 596 4.64e-22 98.2
Msa0547140 AT1G19850.2 49.000 100 49 2 9 107 279 377 2.37e-20 93.2
Msa0547140 AT1G19850.1 49.000 100 49 2 9 107 290 388 2.56e-20 93.2
Msa0547140 AT1G30330.3 44.000 125 66 4 10 133 262 383 4.15e-20 92.4
Msa0547140 AT1G30330.2 44.000 125 66 4 10 133 262 383 4.15e-20 92.4
Msa0547140 AT1G30330.1 44.000 125 66 4 10 133 260 381 4.39e-20 92.4
Msa0547140 AT5G20730.2 42.478 113 64 1 12 124 262 373 1.50e-19 90.9
Msa0547140 AT5G37020.3 42.056 107 60 2 9 114 163 268 2.63e-19 89.7
Msa0547140 AT5G37020.1 42.056 107 60 2 9 114 258 363 2.70e-19 90.1
Msa0547140 AT5G37020.2 42.056 107 60 2 9 114 258 363 2.92e-19 89.7
Msa0547140 AT1G19220.1 40.000 130 67 4 12 138 261 382 1.06e-18 88.2
Msa0547140 ATMG00940.1 52.439 82 36 2 2 82 69 148 1.31e-18 82.4
Msa0547140 AT5G20730.1 42.105 114 64 2 12 124 262 374 3.83e-18 86.7
Msa0547140 AT5G20730.3 42.105 114 64 2 12 124 262 374 4.62e-18 86.3
Msa0547140 AT5G60450.1 34.884 172 91 6 13 167 312 479 7.10e-18 85.5
Msa0547140 AT5G60450.2 34.884 172 91 6 13 167 288 455 7.53e-18 85.5
Msa0547140 AT5G62000.4 42.105 114 63 2 13 126 300 410 1.03e-17 85.1
Msa0547140 AT5G62000.2 42.105 114 63 2 13 126 300 410 1.06e-17 85.1
Msa0547140 AT5G62000.5 42.105 114 63 2 13 126 300 410 1.06e-17 85.1
Msa0547140 AT5G62000.3 42.105 114 63 2 13 126 300 410 1.06e-17 85.1
Msa0547140 AT5G62000.1 42.105 114 63 2 13 126 300 410 1.06e-17 85.1
Msa0547140 AT2G33860.2 38.318 107 63 2 2 107 127 231 3.59e-14 73.9
Msa0547140 AT2G33860.1 36.792 106 66 1 2 107 284 388 4.59e-14 73.9
Msa0547140 AT3G61830.1 36.296 135 73 6 13 144 264 388 1.91e-12 68.9
Msa0547140 AT3G61830.2 36.296 135 73 6 13 144 213 337 2.09e-12 68.6
Msa0547140 AT2G46530.2 41.667 96 50 4 13 107 173 263 2.82e-12 68.2
Msa0547140 AT2G46530.1 41.667 96 50 4 13 107 260 350 3.82e-12 67.8
Msa0547140 AT4G23980.2 41.053 95 52 3 13 107 252 342 4.16e-12 67.8
Msa0547140 AT4G23980.1 41.053 95 52 3 13 107 252 342 4.24e-12 67.8
Msa0547140 AT2G46530.3 41.667 96 50 4 13 107 281 371 4.66e-12 67.8

Find 163 sgRNAs with CRISPR-Local

Find 266 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTATGATCATGTGAAATTTA+AGG 0.117603 4_1:+39905466 Msa0547140:three_prime_UTR
ATTCTTCTTAAAGAAGAATT+TGG 0.141559 4_1:+39902173 Msa0547140:five_prime_UTR
TTCTAGGATTAGTTGGTTTA+TGG 0.143329 4_1:+39903276 Msa0547140:CDS
TCTTTCTTTGTTGGGGTGTT+AGG 0.196783 4_1:+39902233 Msa0547140:five_prime_UTR
TTGAAACTGAAGACTCTTCT+AGG 0.222931 4_1:+39903260 Msa0547140:CDS
ACCCTTGATTTATCACTTCT+TGG 0.257467 4_1:+39904669 Msa0547140:CDS
TTGACAATTCTGACAGATTC+AGG 0.268164 4_1:+39905175 Msa0547140:CDS
TTCTCCGTGGCGCCTTCTTC+AGG 0.276881 4_1:+39903345 Msa0547140:CDS
TCATCTTGTGGATGGACTTC+AGG 0.278431 4_1:+39902945 Msa0547140:five_prime_UTR
CTAATACAAACATTGATCTA+AGG 0.281084 4_1:+39902412 Msa0547140:five_prime_UTR
AACAATGTAAATTGTGGAAT+TGG 0.287100 4_1:+39902978 Msa0547140:five_prime_UTR
AGTTGGAATCTTTCTTTGTT+GGG 0.288375 4_1:+39902225 Msa0547140:five_prime_UTR
GGAAAGCGAGAAAGGGTATT+TGG 0.289545 4_1:+39902293 Msa0547140:five_prime_UTR
TAATAATGTTAACCAAGATT+TGG 0.290265 4_1:+39905660 Msa0547140:three_prime_UTR
CAGGCATGATAAGCTTTCTT+TGG 0.290621 4_1:-39904358 None:intergenic
TGGAATTCGTAGGGCGAAAA+AGG 0.294025 4_1:+39902899 Msa0547140:five_prime_UTR
GGGCCAAACAGCCCCTTCTT+TGG 0.299415 4_1:+39904147 Msa0547140:CDS
TTCTTCAGAGAATCTGAAAA+TGG 0.300038 4_1:-39902062 None:intergenic
AGAAGGCGCCACGGAGAATT+AGG 0.313389 4_1:-39903340 None:intergenic
GGGAACAATTGCTTCTGTTC+AGG 0.316761 4_1:+39903297 Msa0547140:CDS
CACCAGCAACAAGCTTCTTC+TGG 0.317024 4_1:-39902833 None:intergenic
ATGAGGATTCAATGGTGTTC+TGG 0.321716 4_1:+39903217 Msa0547140:CDS
TCTAGGATTAGTTGGTTTAT+GGG 0.340586 4_1:+39903277 Msa0547140:CDS
CGCCGGCACTTGCTTACAAC+AGG 0.344728 4_1:+39902792 Msa0547140:five_prime_UTR
AATTGTGGAATTGGACCTTT+TGG 0.346313 4_1:+39902987 Msa0547140:five_prime_UTR
TGTGGATGGACTTCAGGTTC+AGG 0.346418 4_1:+39902951 Msa0547140:five_prime_UTR
TGGCAACTTGGATTGGATAT+TGG 0.355146 4_1:+39904606 Msa0547140:CDS
AAGTTGGAATCTTTCTTTGT+TGG 0.356899 4_1:+39902224 Msa0547140:five_prime_UTR
TATCATGCCTGTTGAGTATA+GGG 0.358214 4_1:+39904370 Msa0547140:CDS
TAATGAAGCTTCTTCCTTTG+AGG 0.360496 4_1:+39902517 Msa0547140:five_prime_UTR
AAGAAGAAGAAAACAAATTC+AGG 0.362993 4_1:+39901984 Msa0547140:five_prime_UTR
AGCAACAATAAACTTCAGTT+AGG 0.363663 4_1:+39904252 Msa0547140:CDS
ATCATTCACTATGGTTAAAC+AGG 0.363665 4_1:-39902031 None:intergenic
GAAATTTGAATCATTCACTA+TGG 0.365496 4_1:-39902040 None:intergenic
GTGTCTGTCTGTGTTGTTGT+TGG 0.367469 4_1:+39901872 Msa0547140:five_prime_UTR
TTCACCACTGATTCTTTGTA+AGG 0.370999 4_1:+39902443 Msa0547140:five_prime_UTR
GGTTGTTGCTGTTAAATTCA+TGG 0.373821 4_1:+39902464 Msa0547140:five_prime_UTR
TGTTATGGAAGCTGTGAAAT+TGG 0.385027 4_1:+39903105 Msa0547140:exon
TTCTTCTTAAAGAAGAATTT+GGG 0.390863 4_1:+39902174 Msa0547140:five_prime_UTR
AAACCAAAGAAGGGGCTGTT+TGG 0.394320 4_1:-39904150 None:intergenic
AGGATGTGCACGGTGAGCTT+TGG 0.396378 4_1:+39902739 Msa0547140:five_prime_UTR
AAGACTCTTCTAGGATTAGT+TGG 0.399400 4_1:+39903269 Msa0547140:CDS
TTATCATGCCTGTTGAGTAT+AGG 0.406824 4_1:+39904369 Msa0547140:CDS
GAGAATAAGACATTGAGAAA+TGG 0.411485 4_1:+39903035 Msa0547140:five_prime_UTR
GAGTGAGGCTGAAGATGTTA+TGG 0.412147 4_1:+39903090 Msa0547140:exon
CACTCCCGAGTTCTGTGTTA+AGG 0.419777 4_1:+39903174 Msa0547140:CDS
TTCTTCCTCGGTGGAGTGAA+AGG 0.420088 4_1:-39904048 None:intergenic
AGGTGGTGGTGGTGATGGTT+TGG 0.423416 4_1:+39902563 Msa0547140:five_prime_UTR
TTCGCCACCACCGTCTGAAC+AGG 0.425252 4_1:-39902717 None:intergenic
CTAATGCTGCTGAGTTTAGT+TGG 0.428567 4_1:+39904586 Msa0547140:CDS
GCCTACTACTACAGGTTACA+TGG 0.430758 4_1:+39903938 Msa0547140:intron
GGATAGTAAACAACTTCAAA+TGG 0.432142 4_1:-39903142 None:intergenic
ACGGTGAGCTTTGGAAGTTT+AGG 0.437969 4_1:+39902748 Msa0547140:five_prime_UTR
TAAATGTGGGGTCCAAATCT+TGG 0.443251 4_1:-39905672 None:intergenic
TGAGGATTCAATGGTGTTCT+GGG 0.446254 4_1:+39903218 Msa0547140:CDS
AACCAAGAAGTGATAAATCA+AGG 0.446549 4_1:-39904671 None:intergenic
TTCAATGGTGTTCTGGGATG+AGG 0.448575 4_1:+39903224 Msa0547140:CDS
AGAAGAAAGAGTGTTAAAGT+TGG 0.452089 4_1:+39902208 Msa0547140:five_prime_UTR
ACTGAAGTTTATTGTTGCTA+AGG 0.452992 4_1:-39904248 None:intergenic
GATGAAGGTGGTGGTGGTGA+TGG 0.454860 4_1:+39902558 Msa0547140:five_prime_UTR
CATTATGCAAATATGCTTAG+AGG 0.456170 4_1:-39905379 None:intergenic
CAGCTATGGCATGCTTGTGC+TGG 0.456859 4_1:+39902321 Msa0547140:five_prime_UTR
AGCACCTTGATGTGCATAGT+AGG 0.468204 4_1:+39904295 Msa0547140:CDS
TCAAGGTGCTGAATGTTAGT+AGG 0.470920 4_1:-39904282 None:intergenic
AAGTTTAGGCATATTTATAG+AGG 0.471597 4_1:+39902762 Msa0547140:five_prime_UTR
AACCAGAAGAAGCTTGTTGC+TGG 0.473424 4_1:+39902831 Msa0547140:five_prime_UTR
GCTGAGTTTAGTTGGCAACT+TGG 0.473727 4_1:+39904594 Msa0547140:CDS
AACTCGGGAGTGCTAGCTCT+TGG 0.477183 4_1:-39903163 None:intergenic
TCAGAGCTTGGTTATGATGA+AGG 0.480927 4_1:+39902543 Msa0547140:five_prime_UTR
TTGGATGCATCAAACAAAAC+AGG 0.481900 4_1:+39905355 Msa0547140:CDS
ATTGTCAGATAAACCAAAGA+AGG 0.484820 4_1:-39904160 None:intergenic
GTTGGAATCTTTCTTTGTTG+GGG 0.486228 4_1:+39902226 Msa0547140:five_prime_UTR
GGTTATGATGAAGGTGGTGG+TGG 0.486406 4_1:+39902552 Msa0547140:five_prime_UTR
TCACAAAGGTGAACATGGAT+TGG 0.488178 4_1:+39905336 Msa0547140:CDS
GTTTAGTTGGCAACTTGGAT+TGG 0.492268 4_1:+39904599 Msa0547140:CDS
TCAATGAAGGAAAGCGAGAA+AGG 0.493704 4_1:+39902285 Msa0547140:five_prime_UTR
GCAACAATAAACTTCAGTTA+GGG 0.494974 4_1:+39904253 Msa0547140:CDS
AGGGGCTGTTTGGCCCGAGC+TGG 0.496697 4_1:-39904140 None:intergenic
TGTTCAGACGGTGGTGGCGA+AGG 0.501569 4_1:+39902719 Msa0547140:five_prime_UTR
GGTTCTAACAATGTAAATTG+TGG 0.502204 4_1:+39902972 Msa0547140:five_prime_UTR
AGAAGAGTCTTCAGTTTCAA+AGG 0.502379 4_1:-39903257 None:intergenic
GGAGAATTAGGCCAGCGAAT+AGG 0.504468 4_1:-39903328 None:intergenic
TTGATGGTTCATCTTGTGGA+TGG 0.504684 4_1:+39902937 Msa0547140:five_prime_UTR
AGAAGATTCTCTTATCAAGA+TGG 0.506507 4_1:+39905570 Msa0547140:three_prime_UTR
AGGTTGTTGATCCTATTCGC+TGG 0.509848 4_1:+39903317 Msa0547140:CDS
CTATGGCATGCTTGTGCTGG+TGG 0.512929 4_1:+39902324 Msa0547140:five_prime_UTR
GCATGCTTGTGCTGGTGGAA+TGG 0.513876 4_1:+39902329 Msa0547140:five_prime_UTR
AATCTGACGCAAACAATGGT+GGG 0.517008 4_1:+39902628 Msa0547140:five_prime_UTR
AGTAAACAACTTCAAATGGT+TGG 0.519885 4_1:-39903138 None:intergenic
TGTTAAGGCTTCAGCTGTGA+AGG 0.522163 4_1:+39903189 Msa0547140:CDS
CCTTTGAGGAATTCAGAGCT+TGG 0.522861 4_1:+39902531 Msa0547140:five_prime_UTR
ATATTGTTGTTTACTTAATG+TGG 0.522947 4_1:+39905418 Msa0547140:three_prime_UTR
GGCTGAGCCGCCTGTTCAGA+CGG 0.523312 4_1:+39902707 Msa0547140:five_prime_UTR
GAAGAGTCTTCAGTTTCAAA+GGG 0.524779 4_1:-39903256 None:intergenic
AAATAATGATGCATATATCA+TGG 0.525854 4_1:+39905624 Msa0547140:three_prime_UTR
GGAAACCGCAGCTTCTTCCT+CGG 0.526125 4_1:-39904060 None:intergenic
TAATACAAACATTGATCTAA+GGG 0.528805 4_1:+39902413 Msa0547140:five_prime_UTR
CTTTCCAAGGCTTGATTACG+CGG 0.534891 4_1:+39902686 Msa0547140:five_prime_UTR
TGAAGCCTTAACACAGAACT+CGG 0.535150 4_1:-39903179 None:intergenic
TCTTCCTCGGTGGAGTGAAA+GGG 0.536181 4_1:-39904047 None:intergenic
ACCCTGTTGTAAGCAAGTGC+CGG 0.547262 4_1:-39902794 None:intergenic
GCATGTTCAGCATGTCCTTG+TGG 0.548555 4_1:-39902390 None:intergenic
CAGCTGTGAAGGCTGCTATG+AGG 0.549739 4_1:+39903200 Msa0547140:CDS
GATACTAATTCGACCAACCA+CGG 0.553761 4_1:-39904000 None:intergenic
AATTGTGTGTTGGAATTCGT+AGG 0.556594 4_1:+39902889 Msa0547140:five_prime_UTR
ATCGTCTCAGCACAGTACCT+TGG 0.557673 4_1:-39902663 None:intergenic
GCCGCCTGTTCAGACGGTGG+TGG 0.561486 4_1:+39902713 Msa0547140:five_prime_UTR
CAATGAAGGAAAGCGAGAAA+GGG 0.562434 4_1:+39902286 Msa0547140:five_prime_UTR
ATACTAATTCGACCAACCAC+GGG 0.564828 4_1:-39903999 None:intergenic
ATTGGATATTGGTCATTGCA+AGG 0.566046 4_1:+39904617 Msa0547140:CDS
GGAACTCACAAAGGTGAACA+TGG 0.567844 4_1:+39905331 Msa0547140:CDS
GAAGCGTGTTAGCCCGTGGT+TGG 0.567936 4_1:+39903987 Msa0547140:CDS
TTGTCAGATAAACCAAAGAA+GGG 0.569032 4_1:-39904159 None:intergenic
ATAGTAAAGACAAAGAGAAG+TGG 0.569647 4_1:+39904532 Msa0547140:CDS
AGTTTAGGCATATTTATAGA+GGG 0.570327 4_1:+39902763 Msa0547140:five_prime_UTR
GCTTGTTGCTGGTGATTCTG+TGG 0.572232 4_1:+39902842 Msa0547140:five_prime_UTR
ACCAAGAAGTGATAAATCAA+GGG 0.572563 4_1:-39904670 None:intergenic
CAATCTGACGCAAACAATGG+TGG 0.579930 4_1:+39902627 Msa0547140:five_prime_UTR
TCTGACGCAAACAATGGTGG+GGG 0.580642 4_1:+39902630 Msa0547140:five_prime_UTR
AAAGTCTTAGCAAAAGATGC+AGG 0.580849 4_1:-39902600 None:intergenic
CCAAGCTCTGAATTCCTCAA+AGG 0.581047 4_1:-39902531 None:intergenic
AAATCCTACTATGCACATCA+AGG 0.584801 4_1:-39904299 None:intergenic
TGAGTGGAAGAGTGAGAGTG+AGG 0.587276 4_1:+39903075 Msa0547140:five_prime_UTR
ATCTGACGCAAACAATGGTG+GGG 0.587603 4_1:+39902629 Msa0547140:five_prime_UTR
GAGAATGGTGAATTGTGTGT+TGG 0.591957 4_1:+39902879 Msa0547140:five_prime_UTR
GTGCTGAGACGATCTTTCCA+AGG 0.594168 4_1:+39902673 Msa0547140:five_prime_UTR
TTTATAGAGGGACACCGCGC+CGG 0.597722 4_1:+39902775 Msa0547140:five_prime_UTR
TGACGGAAATTGAACATCGA+GGG 0.599791 4_1:-39904100 None:intergenic
TTGCCTGAAAACGTCGGTGA+CGG 0.603464 4_1:-39904117 None:intergenic
TGGAGTCAGAAGATGTTGGA+AGG 0.603517 4_1:+39904646 Msa0547140:CDS
AGGCTGCTATGAGGATTCAA+TGG 0.603915 4_1:+39903209 Msa0547140:CDS
CTCCACCGAGGAAGAAGCTG+CGG 0.604549 4_1:+39904055 Msa0547140:CDS
GGTATTTGGATCCACAGCTA+TGG 0.606132 4_1:+39902307 Msa0547140:five_prime_UTR
CAACACAGACAGACACACTC+CGG 0.610206 4_1:-39901865 None:intergenic
AACCGCAGCTTCTTCCTCGG+TGG 0.613433 4_1:-39904057 None:intergenic
GCCACCACCGTCTGAACAGG+CGG 0.614156 4_1:-39902714 None:intergenic
TTCTTGGAGTCAGAAGATGT+TGG 0.615581 4_1:+39904642 Msa0547140:CDS
AAAGGGCTGAAATGAATGAC+TGG 0.619094 4_1:-39904030 None:intergenic
CCTACTACTACAGGTTACAT+GGG 0.623681 4_1:+39903939 Msa0547140:intron
CTTGGTTATGATGAAGGTGG+TGG 0.625908 4_1:+39902549 Msa0547140:five_prime_UTR
ACTCAATCTGACGCAAACAA+TGG 0.625908 4_1:+39902624 Msa0547140:five_prime_UTR
GACGGAAATTGAACATCGAG+GGG 0.629218 4_1:-39904099 None:intergenic
TCAGCCGCGTAATCAAGCCT+TGG 0.630202 4_1:-39902690 None:intergenic
GCACAAGCATGCCATAGCTG+TGG 0.631502 4_1:-39902318 None:intergenic
ATTGTGTGTTGGAATTCGTA+GGG 0.641411 4_1:+39902890 Msa0547140:five_prime_UTR
ACGTGAAGCGTGTTAGCCCG+TGG 0.646883 4_1:+39903983 Msa0547140:CDS
AATACCTGAAGAAGGCGCCA+CGG 0.652216 4_1:-39903349 None:intergenic
GTTGTAAGCAAGTGCCGGCG+CGG 0.664793 4_1:-39902789 None:intergenic
TGAGCCGCCTGTTCAGACGG+TGG 0.665475 4_1:+39902710 Msa0547140:five_prime_UTR
GGCACTTGCTTACAACAGGG+TGG 0.673448 4_1:+39902796 Msa0547140:five_prime_UTR
GAAGCCTTAACACAGAACTC+GGG 0.673594 4_1:-39903178 None:intergenic
TGGATAACAGGAACTCACAA+AGG 0.673838 4_1:+39905322 Msa0547140:CDS
TGTCAGATAAACCAAAGAAG+GGG 0.674937 4_1:-39904158 None:intergenic
AGCTGGTTGCCTGAAAACGT+CGG 0.679058 4_1:-39904123 None:intergenic
ACAACCTTACAAAGAATCAG+TGG 0.680398 4_1:-39902447 None:intergenic
TTCGCTGGCCTAATTCTCCG+TGG 0.685289 4_1:+39903332 Msa0547140:CDS
GTGACGGAAATTGAACATCG+AGG 0.688190 4_1:-39904101 None:intergenic
GCCGGCACTTGCTTACAACA+GGG 0.691948 4_1:+39902793 Msa0547140:five_prime_UTR
CTGACGCAAACAATGGTGGG+GGG 0.702104 4_1:+39902631 Msa0547140:five_prime_UTR
GAGCTTGGTTATGATGAAGG+TGG 0.703479 4_1:+39902546 Msa0547140:five_prime_UTR
GTGGTGGCGAAGGATGTGCA+CGG 0.739739 4_1:+39902729 Msa0547140:five_prime_UTR
ACGGAAATTGAACATCGAGG+GGG 0.745927 4_1:-39904098 None:intergenic
TCAGCCCTTTCACTCCACCG+AGG 0.764350 4_1:+39904043 Msa0547140:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATGTAAAAATTGAAGTTTTT+TGG - chr4_1:39902130-39902149 None:intergenic 15.0%
!!! ATATGAAGATTTTGAATAAA+GGG - chr4_1:39902155-39902174 None:intergenic 15.0%
!!! AATATGAAGATTTTGAATAA+AGG - chr4_1:39902156-39902175 None:intergenic 15.0%
!! GAAAAAATATGATAAAAGTT+TGG + chr4_1:39904392-39904411 Msa0547140:CDS 15.0%
!!! ATATGAACAGAATATTTTTA+TGG + chr4_1:39905524-39905543 Msa0547140:three_prime_UTR 15.0%
!! ATTCTTCTTAAAGAAGAATT+TGG + chr4_1:39902173-39902192 Msa0547140:five_prime_UTR 20.0%
!! TTCTTCTTAAAGAAGAATTT+GGG + chr4_1:39902174-39902193 Msa0547140:five_prime_UTR 20.0%
!! TAATACAAACATTGATCTAA+GGG + chr4_1:39902413-39902432 Msa0547140:five_prime_UTR 20.0%
!!! ATTGTTTTTCAGTTACAATT+TGG + chr4_1:39903369-39903388 Msa0547140:intron 20.0%
!!! TTGAACACATTTTTACATAA+GGG + chr4_1:39903402-39903421 Msa0547140:intron 20.0%
!!! TTTGTTATTGTTCAGTTTTA+TGG + chr4_1:39903462-39903481 Msa0547140:intron 20.0%
!! TAAAAAAAAAAACCAGAGTT+GGG - chr4_1:39903767-39903786 None:intergenic 20.0%
!!! AAAATAGAGTAAAGTCATTT+TGG - chr4_1:39905045-39905064 None:intergenic 20.0%
!! GAAATGTCTTATATAATAAG+AGG + chr4_1:39905277-39905296 Msa0547140:intron 20.0%
!!! ATATTGTTGTTTACTTAATG+TGG + chr4_1:39905418-39905437 Msa0547140:three_prime_UTR 20.0%
!! TTATGATCATGTGAAATTTA+AGG + chr4_1:39905466-39905485 Msa0547140:three_prime_UTR 20.0%
!!! ATAAGAGAATCTTCTTTTTT+TGG - chr4_1:39905565-39905584 None:intergenic 20.0%
!! AAATAATGATGCATATATCA+TGG + chr4_1:39905624-39905643 Msa0547140:three_prime_UTR 20.0%
! AAGAAGAAGAAAACAAATTC+AGG + chr4_1:39901984-39902003 Msa0547140:five_prime_UTR 25.0%
! GAAATTTGAATCATTCACTA+TGG - chr4_1:39902043-39902062 None:intergenic 25.0%
!!! AAGAAAAAGTGTTTCAATGA+AGG + chr4_1:39902272-39902291 Msa0547140:five_prime_UTR 25.0%
!! AAAACTTTTGAATTCACTTG+AGG - chr4_1:39902363-39902382 None:intergenic 25.0%
! CTAATACAAACATTGATCTA+AGG + chr4_1:39902412-39902431 Msa0547140:five_prime_UTR 25.0%
! AAGTTTAGGCATATTTATAG+AGG + chr4_1:39902762-39902781 Msa0547140:five_prime_UTR 25.0%
! AGTTTAGGCATATTTATAGA+GGG + chr4_1:39902763-39902782 Msa0547140:five_prime_UTR 25.0%
! AACAATGTAAATTGTGGAAT+TGG + chr4_1:39902978-39902997 Msa0547140:five_prime_UTR 25.0%
! AAAAAAGAAAATGCTCCAAA+AGG - chr4_1:39903005-39903024 None:intergenic 25.0%
!! GTTGAACACATTTTTACATA+AGG + chr4_1:39903401-39903420 Msa0547140:intron 25.0%
!! ACTCAATTTTTATGCTTCTT+TGG + chr4_1:39903425-39903444 Msa0547140:intron 25.0%
! GTAAAAAAAAAAACCAGAGT+TGG - chr4_1:39903768-39903787 None:intergenic 25.0%
!! TTTTTACTCTTGTGATTATG+AGG + chr4_1:39903781-39903800 Msa0547140:intron 25.0%
!!! TTTTGTATGCTTGATTTGAT+TGG + chr4_1:39903835-39903854 Msa0547140:intron 25.0%
!!! AAACATCAGTTTTGTCTTTT+TGG + chr4_1:39904447-39904466 Msa0547140:CDS 25.0%
! TTGTATACTAAACTAACAAC+CGG - chr4_1:39904951-39904970 None:intergenic 25.0%
! TAAGTTTCACTATACATTCT+AGG + chr4_1:39904987-39905006 Msa0547140:intron 25.0%
!! TTTTGCTACTGTACAATAAT+GGG - chr4_1:39905084-39905103 None:intergenic 25.0%
!! ATTTTGCTACTGTACAATAA+TGG - chr4_1:39905085-39905104 None:intergenic 25.0%
! TAGCAAAATAGTATTACTCT+TGG + chr4_1:39905096-39905115 Msa0547140:intron 25.0%
! TAGTATTACTCTTGGATTAA+TGG + chr4_1:39905104-39905123 Msa0547140:intron 25.0%
! AGTATTACTCTTGGATTAAT+GGG + chr4_1:39905105-39905124 Msa0547140:intron 25.0%
!!! TGAACAGAATATTTTTATGG+AGG + chr4_1:39905527-39905546 Msa0547140:three_prime_UTR 25.0%
ATCATTCACTATGGTTAAAC+AGG - chr4_1:39902034-39902053 None:intergenic 30.0%
TTCTTCAGAGAATCTGAAAA+TGG - chr4_1:39902065-39902084 None:intergenic 30.0%
!!! GAAGTTTTTTGGTTTTCTTG+TGG - chr4_1:39902119-39902138 None:intergenic 30.0%
!! AGAAGAAAGAGTGTTAAAGT+TGG + chr4_1:39902208-39902227 Msa0547140:five_prime_UTR 30.0%
AAGTTGGAATCTTTCTTTGT+TGG + chr4_1:39902224-39902243 Msa0547140:five_prime_UTR 30.0%
AGTTGGAATCTTTCTTTGTT+GGG + chr4_1:39902225-39902244 Msa0547140:five_prime_UTR 30.0%
! AAAGTTTTCTACTTTCCACA+AGG + chr4_1:39902375-39902394 Msa0547140:five_prime_UTR 30.0%
CAAAAACTTCATCAGTTTCA+GGG - chr4_1:39902494-39902513 None:intergenic 30.0%
! GGTTCTAACAATGTAAATTG+TGG + chr4_1:39902972-39902991 Msa0547140:five_prime_UTR 30.0%
GAGAATAAGACATTGAGAAA+TGG + chr4_1:39903035-39903054 Msa0547140:five_prime_UTR 30.0%
!!! TTGAGAAATGGTTGTTTTGA+TGG + chr4_1:39903047-39903066 Msa0547140:five_prime_UTR 30.0%
!!! TGTTTTGATGGTAATTTGAG+TGG + chr4_1:39903059-39903078 Msa0547140:five_prime_UTR 30.0%
AGTAAACAACTTCAAATGGT+TGG - chr4_1:39903141-39903160 None:intergenic 30.0%
GGATAGTAAACAACTTCAAA+TGG - chr4_1:39903145-39903164 None:intergenic 30.0%
!! TTCTAGGATTAGTTGGTTTA+TGG + chr4_1:39903276-39903295 Msa0547140:CDS 30.0%
!! TCTAGGATTAGTTGGTTTAT+GGG + chr4_1:39903277-39903296 Msa0547140:CDS 30.0%
TGAAAAACAATACCTGAAGA+AGG - chr4_1:39903360-39903379 None:intergenic 30.0%
AGAACAATTAGTAGTATTGC+AGG - chr4_1:39903575-39903594 None:intergenic 30.0%
CATTGCTAGTAATGATATTG+TGG + chr4_1:39903643-39903662 Msa0547140:intron 30.0%
TGAAATAACCACTGTTAACT+AGG + chr4_1:39903805-39903824 Msa0547140:intron 30.0%
GAAATAACCACTGTTAACTA+GGG + chr4_1:39903806-39903825 Msa0547140:intron 30.0%
! TTCACGTTTTGTAGTAAATC+AGG - chr4_1:39903970-39903989 None:intergenic 30.0%
TTGTCAGATAAACCAAAGAA+GGG - chr4_1:39904162-39904181 None:intergenic 30.0%
ATTGTCAGATAAACCAAAGA+AGG - chr4_1:39904163-39904182 None:intergenic 30.0%
ACTGAAGTTTATTGTTGCTA+AGG - chr4_1:39904251-39904270 None:intergenic 30.0%
AGCAACAATAAACTTCAGTT+AGG + chr4_1:39904252-39904271 Msa0547140:CDS 30.0%
GCAACAATAAACTTCAGTTA+GGG + chr4_1:39904253-39904272 Msa0547140:CDS 30.0%
!!! ATTTTTTCCCTATACTCAAC+AGG - chr4_1:39904380-39904399 None:intergenic 30.0%
ATAGTAAAGACAAAGAGAAG+TGG + chr4_1:39904532-39904551 Msa0547140:CDS 30.0%
ACCAAGAAGTGATAAATCAA+GGG - chr4_1:39904673-39904692 None:intergenic 30.0%
AACCAAGAAGTGATAAATCA+AGG - chr4_1:39904674-39904693 None:intergenic 30.0%
!!! GTGTCTTTTGCTAGTTTTTT+TGG + chr4_1:39904924-39904943 Msa0547140:intron 30.0%
!!! TTTTGCTAGTTTTTTTGGTC+CGG + chr4_1:39904929-39904948 Msa0547140:intron 30.0%
GTATTACTCTTGGATTAATG+GGG + chr4_1:39905106-39905125 Msa0547140:intron 30.0%
TATTACTCTTGGATTAATGG+GGG + chr4_1:39905107-39905126 Msa0547140:intron 30.0%
!! AGGATCTTGATTGTTTTTCA+GGG + chr4_1:39905297-39905316 Msa0547140:intron 30.0%
CATTATGCAAATATGCTTAG+AGG - chr4_1:39905382-39905401 None:intergenic 30.0%
! TTCATATTCAGACTTTTCAG+AGG - chr4_1:39905511-39905530 None:intergenic 30.0%
AGAAGATTCTCTTATCAAGA+TGG + chr4_1:39905570-39905589 Msa0547140:three_prime_UTR 30.0%
GTTGGAATCTTTCTTTGTTG+GGG + chr4_1:39902226-39902245 Msa0547140:five_prime_UTR 35.0%
!! TTCACCACTGATTCTTTGTA+AGG + chr4_1:39902443-39902462 Msa0547140:five_prime_UTR 35.0%
ACAACCTTACAAAGAATCAG+TGG - chr4_1:39902450-39902469 None:intergenic 35.0%
! GGTTGTTGCTGTTAAATTCA+TGG + chr4_1:39902464-39902483 Msa0547140:five_prime_UTR 35.0%
GCAAAAACTTCATCAGTTTC+AGG - chr4_1:39902495-39902514 None:intergenic 35.0%
TAATGAAGCTTCTTCCTTTG+AGG + chr4_1:39902517-39902536 Msa0547140:five_prime_UTR 35.0%
AAAGTCTTAGCAAAAGATGC+AGG - chr4_1:39902603-39902622 None:intergenic 35.0%
!! TGATTCTGTGGTGTTTTTGA+GGG + chr4_1:39902854-39902873 Msa0547140:five_prime_UTR 35.0%
AATTGTGTGTTGGAATTCGT+AGG + chr4_1:39902889-39902908 Msa0547140:five_prime_UTR 35.0%
ATTGTGTGTTGGAATTCGTA+GGG + chr4_1:39902890-39902909 Msa0547140:five_prime_UTR 35.0%
! AATTGTGGAATTGGACCTTT+TGG + chr4_1:39902987-39903006 Msa0547140:five_prime_UTR 35.0%
TGTTATGGAAGCTGTGAAAT+TGG + chr4_1:39903105-39903124 Msa0547140:exon 35.0%
GAAGAGTCTTCAGTTTCAAA+GGG - chr4_1:39903259-39903278 None:intergenic 35.0%
AGAAGAGTCTTCAGTTTCAA+AGG - chr4_1:39903260-39903279 None:intergenic 35.0%
TTGAAACTGAAGACTCTTCT+AGG + chr4_1:39903260-39903279 Msa0547140:CDS 35.0%
!! AAGACTCTTCTAGGATTAGT+TGG + chr4_1:39903269-39903288 Msa0547140:CDS 35.0%
!! TTCAGTTTTATGGTCATCTC+TGG + chr4_1:39903472-39903491 Msa0547140:intron 35.0%
TGGATAATCACAAGCAAATG+TGG + chr4_1:39903663-39903682 Msa0547140:intron 35.0%
!! TATCTTTGAGATCGCAATTG+AGG - chr4_1:39903697-39903716 None:intergenic 35.0%
ATTGTTATGCCTACTACTAC+AGG + chr4_1:39903930-39903949 Msa0547140:intron 35.0%
TGTCAGATAAACCAAAGAAG+GGG - chr4_1:39904161-39904180 None:intergenic 35.0%
AAATCCTACTATGCACATCA+AGG - chr4_1:39904302-39904321 None:intergenic 35.0%
TTATCATGCCTGTTGAGTAT+AGG + chr4_1:39904369-39904388 Msa0547140:CDS 35.0%
TATCATGCCTGTTGAGTATA+GGG + chr4_1:39904370-39904389 Msa0547140:CDS 35.0%
! ATTTGTTGCTCAGTGAGTAT+TGG - chr4_1:39904477-39904496 None:intergenic 35.0%
! AGACAAAGAGAAGTGGTTTT+TGG + chr4_1:39904539-39904558 Msa0547140:CDS 35.0%
! TTTTTGGATGCATTTTCAGC+TGG + chr4_1:39904555-39904574 Msa0547140:CDS 35.0%
! TTTTGGATGCATTTTCAGCT+GGG + chr4_1:39904556-39904575 Msa0547140:CDS 35.0%
ATTGGATATTGGTCATTGCA+AGG + chr4_1:39904617-39904636 Msa0547140:CDS 35.0%
ACCCTTGATTTATCACTTCT+TGG + chr4_1:39904669-39904688 Msa0547140:CDS 35.0%
ACACAAAAGCAATACCTGAA+GGG - chr4_1:39904813-39904832 None:intergenic 35.0%
AACACAAAAGCAATACCTGA+AGG - chr4_1:39904814-39904833 None:intergenic 35.0%
ATTACTCTTGGATTAATGGG+GGG + chr4_1:39905108-39905127 Msa0547140:intron 35.0%
TTGACAATTCTGACAGATTC+AGG + chr4_1:39905175-39905194 Msa0547140:CDS 35.0%
!! GAGGATCTTGATTGTTTTTC+AGG + chr4_1:39905296-39905315 Msa0547140:intron 35.0%
TTGGATGCATCAAACAAAAC+AGG + chr4_1:39905355-39905374 Msa0547140:CDS 35.0%
! GTGAGTGAAGTTTTTGCTGA+TGG + chr4_1:39902006-39902025 Msa0547140:five_prime_UTR 40.0%
! TCTTTCTTTGTTGGGGTGTT+AGG + chr4_1:39902233-39902252 Msa0547140:five_prime_UTR 40.0%
TCAATGAAGGAAAGCGAGAA+AGG + chr4_1:39902285-39902304 Msa0547140:five_prime_UTR 40.0%
CAATGAAGGAAAGCGAGAAA+GGG + chr4_1:39902286-39902305 Msa0547140:five_prime_UTR 40.0%
TCAGAGCTTGGTTATGATGA+AGG + chr4_1:39902543-39902562 Msa0547140:five_prime_UTR 40.0%
ACTCAATCTGACGCAAACAA+TGG + chr4_1:39902624-39902643 Msa0547140:five_prime_UTR 40.0%
AATCTGACGCAAACAATGGT+GGG + chr4_1:39902628-39902647 Msa0547140:five_prime_UTR 40.0%
!! GTGATTCTGTGGTGTTTTTG+AGG + chr4_1:39902853-39902872 Msa0547140:five_prime_UTR 40.0%
GAGAATGGTGAATTGTGTGT+TGG + chr4_1:39902879-39902898 Msa0547140:five_prime_UTR 40.0%
!! GGTTTTGATGGTTCATCTTG+TGG + chr4_1:39902933-39902952 Msa0547140:five_prime_UTR 40.0%
! TTGATGGTTCATCTTGTGGA+TGG + chr4_1:39902937-39902956 Msa0547140:five_prime_UTR 40.0%
TGAAGCCTTAACACAGAACT+CGG - chr4_1:39903182-39903201 None:intergenic 40.0%
ATGAGGATTCAATGGTGTTC+TGG + chr4_1:39903217-39903236 Msa0547140:CDS 40.0%
TGAGGATTCAATGGTGTTCT+GGG + chr4_1:39903218-39903237 Msa0547140:CDS 40.0%
AGTTCAATTGTCATACTGCG+AGG + chr4_1:39903604-39903623 Msa0547140:intron 40.0%
CAATCTCGGCAACAATATGA+AGG - chr4_1:39903727-39903746 None:intergenic 40.0%
TCATATTGTTGCCGAGATTG+CGG + chr4_1:39903727-39903746 Msa0547140:intron 40.0%
! TGAACTTCTTTTCCCAACTC+TGG + chr4_1:39903752-39903771 Msa0547140:intron 40.0%
AACATCTCCCTAGTTAACAG+TGG - chr4_1:39903816-39903835 None:intergenic 40.0%
CCTACTACTACAGGTTACAT+GGG + chr4_1:39903939-39903958 Msa0547140:intron 40.0%
ATACTAATTCGACCAACCAC+GGG - chr4_1:39904002-39904021 None:intergenic 40.0%
GATACTAATTCGACCAACCA+CGG - chr4_1:39904003-39904022 None:intergenic 40.0%
AAAGGGCTGAAATGAATGAC+TGG - chr4_1:39904033-39904052 None:intergenic 40.0%
TGACGGAAATTGAACATCGA+GGG - chr4_1:39904103-39904122 None:intergenic 40.0%
AATGCTCCTGCATGTATACA+GGG + chr4_1:39904180-39904199 Msa0547140:intron 40.0%
!! TCAAGGTGCTGAATGTTAGT+AGG - chr4_1:39904285-39904304 None:intergenic 40.0%
CAGGCATGATAAGCTTTCTT+TGG - chr4_1:39904361-39904380 None:intergenic 40.0%
CTAATGCTGCTGAGTTTAGT+TGG + chr4_1:39904586-39904605 Msa0547140:CDS 40.0%
GTTTAGTTGGCAACTTGGAT+TGG + chr4_1:39904599-39904618 Msa0547140:CDS 40.0%
! TGGCAACTTGGATTGGATAT+TGG + chr4_1:39904606-39904625 Msa0547140:CDS 40.0%
! TGGTCATTGCAAGGTTTTCT+TGG + chr4_1:39904626-39904645 Msa0547140:CDS 40.0%
TTCTTGGAGTCAGAAGATGT+TGG + chr4_1:39904642-39904661 Msa0547140:CDS 40.0%
!!! GTTTTTTACCGTGATGCAAC+AGG + chr4_1:39904762-39904781 Msa0547140:intron 40.0%
!! TTTTGAGTAAACAAGCGCCT+TGG + chr4_1:39904832-39904851 Msa0547140:intron 40.0%
TCTGACATATTCAAGGACCA+AGG - chr4_1:39904852-39904871 None:intergenic 40.0%
TGGTCCTTGAATATGTCAGA+CGG + chr4_1:39904852-39904871 Msa0547140:intron 40.0%
AACACCGTCTGACATATTCA+AGG - chr4_1:39904859-39904878 None:intergenic 40.0%
!!! CTTGATTGTTTTTCAGGGCA+TGG + chr4_1:39905302-39905321 Msa0547140:intron 40.0%
!!! TTTTTCAGGGCATGGATAAC+AGG + chr4_1:39905310-39905329 Msa0547140:intron 40.0%
TGGATAACAGGAACTCACAA+AGG + chr4_1:39905322-39905341 Msa0547140:CDS 40.0%
TCACAAAGGTGAACATGGAT+TGG + chr4_1:39905336-39905355 Msa0547140:CDS 40.0%
! GTGTCTGTCTGTGTTGTTGT+TGG + chr4_1:39901872-39901891 Msa0547140:five_prime_UTR 45.0%
!! GGAAAGCGAGAAAGGGTATT+TGG + chr4_1:39902293-39902312 Msa0547140:five_prime_UTR 45.0%
GGTATTTGGATCCACAGCTA+TGG + chr4_1:39902307-39902326 Msa0547140:five_prime_UTR 45.0%
!! CCAAGCTCTGAATTCCTCAA+AGG - chr4_1:39902534-39902553 None:intergenic 45.0%
! CCTTTGAGGAATTCAGAGCT+TGG + chr4_1:39902531-39902550 Msa0547140:five_prime_UTR 45.0%
GAGCTTGGTTATGATGAAGG+TGG + chr4_1:39902546-39902565 Msa0547140:five_prime_UTR 45.0%
CTTGGTTATGATGAAGGTGG+TGG + chr4_1:39902549-39902568 Msa0547140:five_prime_UTR 45.0%
CAATCTGACGCAAACAATGG+TGG + chr4_1:39902627-39902646 Msa0547140:five_prime_UTR 45.0%
ATCTGACGCAAACAATGGTG+GGG + chr4_1:39902629-39902648 Msa0547140:five_prime_UTR 45.0%
CTTTCCAAGGCTTGATTACG+CGG + chr4_1:39902686-39902705 Msa0547140:five_prime_UTR 45.0%
!! ACGGTGAGCTTTGGAAGTTT+AGG + chr4_1:39902748-39902767 Msa0547140:five_prime_UTR 45.0%
!! AACCAGAAGAAGCTTGTTGC+TGG + chr4_1:39902831-39902850 Msa0547140:five_prime_UTR 45.0%
!! GTGTTTTTGAGGGCTGAGAA+TGG + chr4_1:39902864-39902883 Msa0547140:five_prime_UTR 45.0%
TGGAATTCGTAGGGCGAAAA+AGG + chr4_1:39902899-39902918 Msa0547140:five_prime_UTR 45.0%
GGAATTCGTAGGGCGAAAAA+GGG + chr4_1:39902900-39902919 Msa0547140:five_prime_UTR 45.0%
! TCATCTTGTGGATGGACTTC+AGG + chr4_1:39902945-39902964 Msa0547140:five_prime_UTR 45.0%
GAGTGAGGCTGAAGATGTTA+TGG + chr4_1:39903090-39903109 Msa0547140:exon 45.0%
GAAGCCTTAACACAGAACTC+GGG - chr4_1:39903181-39903200 None:intergenic 45.0%
TGTTAAGGCTTCAGCTGTGA+AGG + chr4_1:39903189-39903208 Msa0547140:CDS 45.0%
AGGCTGCTATGAGGATTCAA+TGG + chr4_1:39903209-39903228 Msa0547140:CDS 45.0%
!! TTCAATGGTGTTCTGGGATG+AGG + chr4_1:39903224-39903243 Msa0547140:CDS 45.0%
GGGAACAATTGCTTCTGTTC+AGG + chr4_1:39903297-39903316 Msa0547140:CDS 45.0%
AGGTTGTTGATCCTATTCGC+TGG + chr4_1:39903317-39903336 Msa0547140:CDS 45.0%
ATGGTCATCTCTGGTGTTTG+TGG + chr4_1:39903481-39903500 Msa0547140:intron 45.0%
AGAAGTTCACACCGCAATCT+CGG - chr4_1:39903741-39903760 None:intergenic 45.0%
GCCTACTACTACAGGTTACA+TGG + chr4_1:39903938-39903957 Msa0547140:intron 45.0%
CCCATGTAACCTGTAGTAGT+AGG - chr4_1:39903942-39903961 None:intergenic 45.0%
ACGGAAATTGAACATCGAGG+GGG - chr4_1:39904101-39904120 None:intergenic 45.0%
GACGGAAATTGAACATCGAG+GGG - chr4_1:39904102-39904121 None:intergenic 45.0%
! GTGACGGAAATTGAACATCG+AGG - chr4_1:39904104-39904123 None:intergenic 45.0%
AAACCAAAGAAGGGGCTGTT+TGG - chr4_1:39904153-39904172 None:intergenic 45.0%
CAATGCTCCTGCATGTATAC+AGG + chr4_1:39904179-39904198 Msa0547140:intron 45.0%
AGCACCTTGATGTGCATAGT+AGG + chr4_1:39904295-39904314 Msa0547140:CDS 45.0%
! GATGCATTTTCAGCTGGGAA+AGG + chr4_1:39904561-39904580 Msa0547140:CDS 45.0%
GCTGAGTTTAGTTGGCAACT+TGG + chr4_1:39904594-39904613 Msa0547140:CDS 45.0%
TGGAGTCAGAAGATGTTGGA+AGG + chr4_1:39904646-39904665 Msa0547140:CDS 45.0%
! ACAGGTGCTGTGAAGCAAAT+CGG + chr4_1:39904780-39904799 Msa0547140:intron 45.0%
AAATCGGTGAAGAACCCTTC+AGG + chr4_1:39904796-39904815 Msa0547140:intron 45.0%
TCAGGCAGCAAAAATGTGAG+AGG + chr4_1:39905193-39905212 Msa0547140:CDS 45.0%
CAGGCAGCAAAAATGTGAGA+GGG + chr4_1:39905194-39905213 Msa0547140:CDS 45.0%
GGAACTCACAAAGGTGAACA+TGG + chr4_1:39905331-39905350 Msa0547140:CDS 45.0%
GCATGTTCAGCATGTCCTTG+TGG - chr4_1:39902393-39902412 None:intergenic 50.0%
! GGTTATGATGAAGGTGGTGG+TGG + chr4_1:39902552-39902571 Msa0547140:five_prime_UTR 50.0%
TCTGACGCAAACAATGGTGG+GGG + chr4_1:39902630-39902649 Msa0547140:five_prime_UTR 50.0%
ATCGTCTCAGCACAGTACCT+TGG - chr4_1:39902666-39902685 None:intergenic 50.0%
GTGCTGAGACGATCTTTCCA+AGG + chr4_1:39902673-39902692 Msa0547140:five_prime_UTR 50.0%
ACCCTGTTGTAAGCAAGTGC+CGG - chr4_1:39902797-39902816 None:intergenic 50.0%
CACCAGCAACAAGCTTCTTC+TGG - chr4_1:39902836-39902855 None:intergenic 50.0%
! GCTTGTTGCTGGTGATTCTG+TGG + chr4_1:39902842-39902861 Msa0547140:five_prime_UTR 50.0%
CGTAGGGCGAAAAAGGGAAT+CGG + chr4_1:39902906-39902925 Msa0547140:five_prime_UTR 50.0%
!!! GGAATCGGTGGTGGTTTTGA+TGG + chr4_1:39902921-39902940 Msa0547140:five_prime_UTR 50.0%
! TGTGGATGGACTTCAGGTTC+AGG + chr4_1:39902951-39902970 Msa0547140:five_prime_UTR 50.0%
TGAGTGGAAGAGTGAGAGTG+AGG + chr4_1:39903075-39903094 Msa0547140:five_prime_UTR 50.0%
CACTCCCGAGTTCTGTGTTA+AGG + chr4_1:39903174-39903193 Msa0547140:CDS 50.0%
GGAGAATTAGGCCAGCGAAT+AGG - chr4_1:39903331-39903350 None:intergenic 50.0%
!! AATACCTGAAGAAGGCGCCA+CGG - chr4_1:39903352-39903371 None:intergenic 50.0%
TCTTCCTCGGTGGAGTGAAA+GGG - chr4_1:39904050-39904069 None:intergenic 50.0%
TTCTTCCTCGGTGGAGTGAA+AGG - chr4_1:39904051-39904070 None:intergenic 50.0%
! TTTCCGTCACCGACGTTTTC+AGG + chr4_1:39904114-39904133 Msa0547140:CDS 50.0%
TTGCCTGAAAACGTCGGTGA+CGG - chr4_1:39904120-39904139 None:intergenic 50.0%
AGCTGGTTGCCTGAAAACGT+CGG - chr4_1:39904126-39904145 None:intergenic 50.0%
!! ACGTTTTCAGGCAACCAGCT+CGG + chr4_1:39904126-39904145 Msa0547140:CDS 50.0%
TCCTCCAAATTGAGCATGCC+TGG - chr4_1:39904208-39904227 None:intergenic 50.0%
TCACAGCACCTGTTGCATCA+CGG - chr4_1:39904773-39904792 None:intergenic 50.0%
GCACAAGCATGCCATAGCTG+TGG - chr4_1:39902321-39902340 None:intergenic 55.0%
CAGCTATGGCATGCTTGTGC+TGG + chr4_1:39902321-39902340 Msa0547140:five_prime_UTR 55.0%
CTATGGCATGCTTGTGCTGG+TGG + chr4_1:39902324-39902343 Msa0547140:five_prime_UTR 55.0%
! GCATGCTTGTGCTGGTGGAA+TGG + chr4_1:39902329-39902348 Msa0547140:five_prime_UTR 55.0%
!! GATGAAGGTGGTGGTGGTGA+TGG + chr4_1:39902558-39902577 Msa0547140:five_prime_UTR 55.0%
!! AGGTGGTGGTGGTGATGGTT+TGG + chr4_1:39902563-39902582 Msa0547140:five_prime_UTR 55.0%
! CTGACGCAAACAATGGTGGG+GGG + chr4_1:39902631-39902650 Msa0547140:five_prime_UTR 55.0%
! GTGGGGGGTTTTCTGTTCCA+AGG + chr4_1:39902646-39902665 Msa0547140:five_prime_UTR 55.0%
TCAGCCGCGTAATCAAGCCT+TGG - chr4_1:39902693-39902712 None:intergenic 55.0%
AGGATGTGCACGGTGAGCTT+TGG + chr4_1:39902739-39902758 Msa0547140:five_prime_UTR 55.0%
TTTATAGAGGGACACCGCGC+CGG + chr4_1:39902775-39902794 Msa0547140:five_prime_UTR 55.0%
GCCGGCACTTGCTTACAACA+GGG + chr4_1:39902793-39902812 Msa0547140:five_prime_UTR 55.0%
GGCACTTGCTTACAACAGGG+TGG + chr4_1:39902796-39902815 Msa0547140:five_prime_UTR 55.0%
AGGGCGAAAAAGGGAATCGG+TGG + chr4_1:39902909-39902928 Msa0547140:five_prime_UTR 55.0%
GCGAAAAAGGGAATCGGTGG+TGG + chr4_1:39902912-39902931 Msa0547140:five_prime_UTR 55.0%
!! AACTCGGGAGTGCTAGCTCT+TGG - chr4_1:39903166-39903185 None:intergenic 55.0%
CAGCTGTGAAGGCTGCTATG+AGG + chr4_1:39903200-39903219 Msa0547140:CDS 55.0%
! TTCGCTGGCCTAATTCTCCG+TGG + chr4_1:39903332-39903351 Msa0547140:CDS 55.0%
!! AGAAGGCGCCACGGAGAATT+AGG - chr4_1:39903343-39903362 None:intergenic 55.0%
GGAAACCGCAGCTTCTTCCT+CGG - chr4_1:39904063-39904082 None:intergenic 55.0%
!! CGTTTTCAGGCAACCAGCTC+GGG + chr4_1:39904127-39904146 Msa0547140:CDS 55.0%
CTGGCTCCCTGTATACATGC+AGG - chr4_1:39904189-39904208 None:intergenic 55.0%
CTGCATGTATACAGGGAGCC+AGG + chr4_1:39904187-39904206 Msa0547140:intron 55.0%
GGAGCCAGGCATGCTCAATT+TGG + chr4_1:39904201-39904220 Msa0547140:intron 55.0%
GCCAGGCATGCTCAATTTGG+AGG + chr4_1:39904204-39904223 Msa0547140:intron 55.0%
TTCGCCACCACCGTCTGAAC+AGG - chr4_1:39902720-39902739 None:intergenic 60.0%
! TGTTCAGACGGTGGTGGCGA+AGG + chr4_1:39902719-39902738 Msa0547140:five_prime_UTR 60.0%
! GTGGTGGCGAAGGATGTGCA+CGG + chr4_1:39902729-39902748 Msa0547140:five_prime_UTR 60.0%
!! GTTGTAAGCAAGTGCCGGCG+CGG - chr4_1:39902792-39902811 None:intergenic 60.0%
CGCCGGCACTTGCTTACAAC+AGG + chr4_1:39902792-39902811 Msa0547140:five_prime_UTR 60.0%
TTCTCCGTGGCGCCTTCTTC+AGG + chr4_1:39903345-39903364 Msa0547140:CDS 60.0%
ACGTGAAGCGTGTTAGCCCG+TGG + chr4_1:39903983-39904002 Msa0547140:CDS 60.0%
GAAGCGTGTTAGCCCGTGGT+TGG + chr4_1:39903987-39904006 Msa0547140:CDS 60.0%
TCAGCCCTTTCACTCCACCG+AGG + chr4_1:39904043-39904062 Msa0547140:CDS 60.0%
CTCCACCGAGGAAGAAGCTG+CGG + chr4_1:39904055-39904074 Msa0547140:CDS 60.0%
AACCGCAGCTTCTTCCTCGG+TGG - chr4_1:39904060-39904079 None:intergenic 60.0%
AGGGGGAAGTCGAGGTTTGG+TGG - chr4_1:39904084-39904103 None:intergenic 60.0%
TCGAGGGGGAAGTCGAGGTT+TGG - chr4_1:39904087-39904106 None:intergenic 60.0%
GAACATCGAGGGGGAAGTCG+AGG - chr4_1:39904092-39904111 None:intergenic 60.0%
GGGCCAAACAGCCCCTTCTT+TGG + chr4_1:39904147-39904166 Msa0547140:CDS 60.0%
GGCTGAGCCGCCTGTTCAGA+CGG + chr4_1:39902707-39902726 Msa0547140:five_prime_UTR 65.0%
TGAGCCGCCTGTTCAGACGG+TGG + chr4_1:39902710-39902729 Msa0547140:five_prime_UTR 65.0%
GCCACCACCGTCTGAACAGG+CGG - chr4_1:39902717-39902736 None:intergenic 65.0%
GCCGCCTGTTCAGACGGTGG+TGG + chr4_1:39902713-39902732 Msa0547140:five_prime_UTR 70.0%
AGGGGCTGTTTGGCCCGAGC+TGG - chr4_1:39904143-39904162 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 39901866 39905681 39901866 ID=Msa0547140;Name=Msa0547140
chr4_1 mRNA 39901866 39905681 39901866 ID=Msa0547140-mRNA-1;Parent=Msa0547140;Name=Msa0547140-mRNA-1;_AED=0.06;_eAED=0.06;_QI=1243|0.4|0.33|1|0.8|0.66|6|282|361
chr4_1 exon 39901866 39903366 39901866 ID=Msa0547140-mRNA-1:exon:10172;Parent=Msa0547140-mRNA-1
chr4_1 exon 39903952 39904168 39903952 ID=Msa0547140-mRNA-1:exon:10173;Parent=Msa0547140-mRNA-1
chr4_1 exon 39904241 39904468 39904241 ID=Msa0547140-mRNA-1:exon:10174;Parent=Msa0547140-mRNA-1
chr4_1 exon 39904514 39904761 39904514 ID=Msa0547140-mRNA-1:exon:10175;Parent=Msa0547140-mRNA-1
chr4_1 exon 39905163 39905215 39905163 ID=Msa0547140-mRNA-1:exon:10176;Parent=Msa0547140-mRNA-1
chr4_1 exon 39905318 39905681 39905318 ID=Msa0547140-mRNA-1:exon:10177;Parent=Msa0547140-mRNA-1
chr4_1 five_prime_UTR 39901866 39903108 39901866 ID=Msa0547140-mRNA-1:five_prime_utr;Parent=Msa0547140-mRNA-1
chr4_1 CDS 39903109 39903366 39903109 ID=Msa0547140-mRNA-1:cds;Parent=Msa0547140-mRNA-1
chr4_1 CDS 39903952 39904168 39903952 ID=Msa0547140-mRNA-1:cds;Parent=Msa0547140-mRNA-1
chr4_1 CDS 39904241 39904468 39904241 ID=Msa0547140-mRNA-1:cds;Parent=Msa0547140-mRNA-1
chr4_1 CDS 39904514 39904761 39904514 ID=Msa0547140-mRNA-1:cds;Parent=Msa0547140-mRNA-1
chr4_1 CDS 39905163 39905215 39905163 ID=Msa0547140-mRNA-1:cds;Parent=Msa0547140-mRNA-1
chr4_1 CDS 39905318 39905399 39905318 ID=Msa0547140-mRNA-1:cds;Parent=Msa0547140-mRNA-1
chr4_1 three_prime_UTR 39905400 39905681 39905400 ID=Msa0547140-mRNA-1:three_prime_utr;Parent=Msa0547140-mRNA-1
Gene Sequence

>Msa0547140

ATGGAAGCTGTGAAATTGGCTGCTAATAACCAACCATTTGAAGTTGTTTACTATCCAAGAGCTAGCACTCCCGAGTTCTGTGTTAAGGCTTCAGCTGTGAAGGCTGCTATGAGGATTCAATGGTGTTCTGGGATGAGGTTCAAGATGCCCTTTGAAACTGAAGACTCTTCTAGGATTAGTTGGTTTATGGGAACAATTGCTTCTGTTCAGGTTGTTGATCCTATTCGCTGGCCTAATTCTCCGTGGCGCCTTCTTCAGGTTACATGGGATGAGCCTGATTTACTACAAAACGTGAAGCGTGTTAGCCCGTGGTTGGTCGAATTAGTATCAAATGTACCAGTCATTCATTTCAGCCCTTTCACTCCACCGAGGAAGAAGCTGCGGTTTCCACCAAACCTCGACTTCCCCCTCGATGTTCAATTTCCGTCACCGACGTTTTCAGGCAACCAGCTCGGGCCAAACAGCCCCTTCTTTGATTTCAACCTTAGCAACAATAAACTTCAGTTAGGGTTGTTTCCTACTAACATTCAGCACCTTGATGTGCATAGTAGGATTTCAAATAGCAACAACATGATGAACAACAATGATAAGACCAAAGAAAGCTTATCATGCCTGTTGAGTATAGGGAAAAAATATGATAAAAGTTTGGAGAAATCTCAATCTGATGACAATGTTGTTAAGAAACATCAGTTTTGTCTTTTTGAAAAAAATTCAGTAGATGATAGTAAAGACAAAGAGAAGTGGTTTTTGGATGCATTTTCAGCTGGGAAAGGTTCTAATGCTGCTGAGTTTAGTTGGCAACTTGGATTGGATATTGGTCATTGCAAGGTTTTCTTGGAGTCAGAAGATGTTGGAAGGACCCTTGATTTATCACTTCTTGGTTCTTATGAAGAGCTCTACAAGAAACTTGCTAACATGTTTGATTTAGAAAAGTCTGAGATGTTGAATCATACAGCTAAAAGATTGACAATTCTGACAGATTCAGGCAGCAAAAATGTGAGAGGGGCATGGATAACAGGAACTCACAAAGGTGAACATGGATTGGATGCATCAAACAAAACAGGACCTCTAAGCATATTTGCATAA
Protein sequence

>Msa0547140

MEAVKLAANNQPFEVVYYPRASTPEFCVKASAVKAAMRIQWCSGMRFKMPFETEDSSRISWFMGTIASVQVVDPIRWPNSPWRLLQVTWDEPDLLQNVKRVSPWLVELVSNVPVIHFSPFTPPRKKLRFPPNLDFPLDVQFPSPTFSGNQLGPNSPFFDFNLSNNKLQLGLFPTNIQHLDVHSRISNSNNMMNNNDKTKESLSCLLSIGKKYDKSLEKSQSDDNVVKKHQFCLFEKNSVDDSKDKEKWFLDAFSAGKGSNAAEFSWQLGLDIGHCKVFLESEDVGRTLDLSLLGSYEELYKKLANMFDLEKSEMLNHTAKRLTILTDSGSKNVRGAWITGTHKGEHGLDASNKTGPLSIFA*