AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0316800


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0316800 MtrunA17_Chr2g0289601 93.333 75 4 1 28 101 136 210 7.03e-25 92.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 24 sgRNAs with CRISPR-Local

Find 33 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGAATATGAATAAGAATTT+TGG 0.192899 2_4:-14056304 Msa0316800:CDS
AGAAAGGATTTGGGGTGTTT+GGG 0.237919 2_4:-14056116 Msa0316800:CDS
AGAAAATTTGTGAGTTTCAT+TGG 0.262073 2_4:-14056139 Msa0316800:CDS
GAGAAAGGATTTGGGGTGTT+TGG 0.279385 2_4:-14056117 Msa0316800:CDS
GTTTCATTGGAGAAAGGATT+TGG 0.305166 2_4:-14056126 Msa0316800:CDS
TTTCATTGGAGAAAGGATTT+GGG 0.320194 2_4:-14056125 Msa0316800:CDS
GTTGCTGCTGATGAAGAAAA+AGG 0.344045 2_4:-14056169 Msa0316800:CDS
AAGAGAAGAGGAACAAGAAA+GGG 0.446012 2_4:-14056336 Msa0316800:CDS
AAAGAGAAGAGGAACAAGAA+AGG 0.446393 2_4:-14056337 Msa0316800:CDS
TGGTTGGTGCAGAGTGGTAA+AGG 0.452437 2_4:-14056382 Msa0316800:intron
TTGTGAGTTTCATTGGAGAA+AGG 0.453786 2_4:-14056132 Msa0316800:CDS
AGTGGTAAAGGTGGAAGGAA+AGG 0.462740 2_4:-14056370 Msa0316800:CDS
GTGGTAAAGGTGGAAGGAAA+GGG 0.491830 2_4:-14056369 Msa0316800:CDS
AAGGTGGAAGGAAAGGGAAA+GGG 0.501120 2_4:-14056363 Msa0316800:CDS
AGTAATAGTAGTAACAATAG+TGG 0.510346 2_4:-14056220 Msa0316800:CDS
AACTACTGAGTTAGAATCTG+TGG 0.517490 2_4:-14056251 Msa0316800:CDS
GAAAGGATTTGGGGTGTTTG+GGG 0.530002 2_4:-14056115 Msa0316800:CDS
GGAAAGGGAAGAAAGAGAAG+AGG 0.541545 2_4:-14056348 Msa0316800:CDS
AATAGTAGTAACAATAGTGG+TGG 0.552263 2_4:-14056217 Msa0316800:CDS
TTCATTGGAGAAAGGATTTG+GGG 0.576638 2_4:-14056124 Msa0316800:CDS
AAAGGTGGAAGGAAAGGGAA+AGG 0.578469 2_4:-14056364 Msa0316800:CDS
TGCAGAGTGGTAAAGGTGGA+AGG 0.580401 2_4:-14056375 Msa0316800:intron
ACTACTGAGTTAGAATCTGT+GGG 0.616892 2_4:-14056250 Msa0316800:CDS
TTGGTGCAGAGTGGTAAAGG+TGG 0.641768 2_4:-14056379 Msa0316800:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAGAATATGAATAAGAATTT+TGG - chr2_4:14056311-14056330 Msa0316800:CDS 15.0%
! GTCAAATAATTAAAGCTACT+GGG + chr2_4:14056165-14056184 None:intergenic 25.0%
! AGTCAAATAATTAAAGCTAC+TGG + chr2_4:14056166-14056185 None:intergenic 25.0%
! AGTAATAGTAGTAACAATAG+TGG - chr2_4:14056395-14056414 Msa0316800:intron 25.0%
! AGAAAATTTGTGAGTTTCAT+TGG - chr2_4:14056476-14056495 Msa0316800:intron 25.0%
!! AGAATTTTGGTGAGTTGAAT+CGG - chr2_4:14056324-14056343 Msa0316800:CDS 30.0%
AATAGTAGTAACAATAGTGG+TGG - chr2_4:14056398-14056417 Msa0316800:intron 30.0%
TTTCATTGGAGAAAGGATTT+GGG - chr2_4:14056490-14056509 Msa0316800:intron 30.0%
! TCATGTATGAGTTGACGATT+CGG + chr2_4:14056191-14056210 None:intergenic 35.0%
! TGAGATCATTGATGCTTTTG+AGG - chr2_4:14056208-14056227 Msa0316800:CDS 35.0%
AAAGAGAAGAGGAACAAGAA+AGG - chr2_4:14056278-14056297 Msa0316800:CDS 35.0%
AAGAGAAGAGGAACAAGAAA+GGG - chr2_4:14056279-14056298 Msa0316800:CDS 35.0%
AACTACTGAGTTAGAATCTG+TGG - chr2_4:14056364-14056383 Msa0316800:CDS 35.0%
ACTACTGAGTTAGAATCTGT+GGG - chr2_4:14056365-14056384 Msa0316800:CDS 35.0%
TTGCTGCTGATGAAGAAAAA+GGG - chr2_4:14056447-14056466 Msa0316800:intron 35.0%
TTGTGAGTTTCATTGGAGAA+AGG - chr2_4:14056483-14056502 Msa0316800:intron 35.0%
GTTTCATTGGAGAAAGGATT+TGG - chr2_4:14056489-14056508 Msa0316800:intron 35.0%
TTCATTGGAGAAAGGATTTG+GGG - chr2_4:14056491-14056510 Msa0316800:intron 35.0%
!! TCATTGATGCTTTTGAGGAG+TGG - chr2_4:14056213-14056232 Msa0316800:CDS 40.0%
GTTGCTGCTGATGAAGAAAA+AGG - chr2_4:14056446-14056465 Msa0316800:intron 40.0%
! AGAAAGGATTTGGGGTGTTT+GGG - chr2_4:14056499-14056518 Msa0316800:intron 40.0%
!! TGATGCTTTTGAGGAGTGGT+TGG - chr2_4:14056217-14056236 Msa0316800:CDS 45.0%
AGTGGTAAAGGTGGAAGGAA+AGG - chr2_4:14056245-14056264 Msa0316800:CDS 45.0%
GTGGTAAAGGTGGAAGGAAA+GGG - chr2_4:14056246-14056265 Msa0316800:CDS 45.0%
AAAGGTGGAAGGAAAGGGAA+AGG - chr2_4:14056251-14056270 Msa0316800:CDS 45.0%
AAGGTGGAAGGAAAGGGAAA+GGG - chr2_4:14056252-14056271 Msa0316800:CDS 45.0%
GGAAAGGGAAGAAAGAGAAG+AGG - chr2_4:14056267-14056286 Msa0316800:CDS 45.0%
! GAGAAAGGATTTGGGGTGTT+TGG - chr2_4:14056498-14056517 Msa0316800:intron 45.0%
! GAAAGGATTTGGGGTGTTTG+GGG - chr2_4:14056500-14056519 Msa0316800:intron 45.0%
! TGGTTGGTGCAGAGTGGTAA+AGG - chr2_4:14056233-14056252 Msa0316800:CDS 50.0%
TTGGTGCAGAGTGGTAAAGG+TGG - chr2_4:14056236-14056255 Msa0316800:CDS 50.0%
TGCAGAGTGGTAAAGGTGGA+AGG - chr2_4:14056240-14056259 Msa0316800:CDS 50.0%
! GAGGAGTGGTTGGTGCAGAG+TGG - chr2_4:14056227-14056246 Msa0316800:CDS 60.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 14056111 14056526 14056111 ID=Msa0316800;Name=Msa0316800
chr2_4 mRNA 14056111 14056526 14056111 ID=Msa0316800-mRNA-1;Parent=Msa0316800;Name=Msa0316800-mRNA-1;_AED=0.37;_eAED=0.42;_QI=0|0|0|1|1|1|2|0|101
chr2_4 exon 14056503 14056526 14056503 ID=Msa0316800-mRNA-1:exon:5348;Parent=Msa0316800-mRNA-1
chr2_4 exon 14056111 14056392 14056111 ID=Msa0316800-mRNA-1:exon:5347;Parent=Msa0316800-mRNA-1
chr2_4 CDS 14056503 14056526 14056503 ID=Msa0316800-mRNA-1:cds;Parent=Msa0316800-mRNA-1
chr2_4 CDS 14056111 14056392 14056111 ID=Msa0316800-mRNA-1:cds;Parent=Msa0316800-mRNA-1
Gene Sequence

>Msa0316800

ATGCAACTCTGTTCAACTGTAGCTAGTGGTAAAGGTGGAAGGAAAGGGAAAGGGAAGAAAGAGAAGAGGAACAAGAAAGGGTTTGAGAATAAGAATATGAATAAGAATTTTGGTGAGTTGAATCGGTCTGTGTTGAACGAGTCAACTACTGAGTTAGAATCTGTGGGTGAAAGTAGTAATAGTAGTAACAATAGTGGTGGAAGTGAAAATAGTGAAAAAGTTGTTGTTGCTGCTGATGAAGAAAAAGGGTCAATGAGAAAATTTGTGAGTTTCATTGGAGAAAGGATTTGGGGTGTTTGGGGATAG

Protein sequence

>Msa0316800

MQLCSTVASGKGGRKGKGKKEKRNKKGFENKNMNKNFGELNRSVLNESTTELESVGESSNSSNNSGGSENSEKVVVAADEEKGSMRKFVSFIGERIWGVWG*