AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0310440

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0310440 MtrunA17_Chr2g0279151 98.221 281 5 0 1 281 1 281 0.0 571
Msa0310440 MtrunA17_Chr4g0050381 79.004 281 55 1 1 281 1 277 6.70e-169 468
Msa0310440 MtrunA17_Chr5g0426141 74.823 282 70 1 1 281 1 282 1.67e-154 432
Msa0310440 MtrunA17_Chr3g0115851 73.404 282 74 1 1 281 1 282 3.72e-154 431
Msa0310440 MtrunA17_Chr2g0317021 61.049 267 103 1 16 281 67 333 7.86e-112 326
Msa0310440 MtrunA17_Chr8g0360351 61.255 271 104 1 11 280 11 281 3.56e-110 320
Msa0310440 MtrunA17_Chr4g0051661 54.217 249 113 1 32 280 150 397 9.27e-88 266
Msa0310440 MtrunA17_Chr1g0150451 46.183 262 134 5 22 280 66 323 2.03e-70 220
Msa0310440 MtrunA17_Chr3g0136711 45.660 265 131 6 22 280 23 280 5.03e-69 216
Msa0310440 MtrunA17_Chr1g0212961 41.729 266 141 7 22 280 79 337 1.41e-64 206
Msa0310440 MtrunA17_Chr3g0126511 45.149 268 140 5 16 280 77 340 1.89e-64 206
Msa0310440 MtrunA17_Chr3g0105371 37.052 251 142 8 41 281 95 339 3.65e-39 139
Msa0310440 MtrunA17_Chr5g0430481 38.776 245 135 7 47 281 136 375 4.77e-39 140
Msa0310440 MtrunA17_Chr3g0105351 37.302 252 142 8 40 281 70 315 1.57e-38 137
Msa0310440 MtrunA17_Chr1g0187481 32.453 265 153 5 23 280 32 277 2.49e-36 130
Msa0310440 MtrunA17_Chr1g0194191 34.800 250 134 7 51 278 105 347 3.82e-36 132
Msa0310440 MtrunA17_Chr5g0407161 34.498 229 132 5 64 280 123 345 9.78e-36 131
Msa0310440 MtrunA17_Chr1g0166481 34.831 267 144 5 21 280 30 273 2.87e-35 127
Msa0310440 MtrunA17_Chr3g0144111 36.187 257 111 8 47 280 1 227 1.30e-34 125
Msa0310440 MtrunA17_Chr4g0070871 37.743 257 115 7 65 281 253 504 6.28e-34 129
Msa0310440 MtrunA17_Chr3g0138281 35.345 232 131 5 64 281 120 346 2.24e-33 125
Msa0310440 MtrunA17_Chr2g0293811 33.898 236 128 7 65 278 100 329 8.07e-33 123
Msa0310440 MtrunA17_Chr4g0065141 32.950 261 142 9 47 281 98 351 9.48e-33 124
Msa0310440 MtrunA17_Chr1g0171571 32.770 296 166 10 8 281 361 645 2.77e-31 122
Msa0310440 MtrunA17_Chr6g0483411 34.286 245 127 9 66 281 80 319 2.63e-30 115
Msa0310440 MtrunA17_Chr2g0299981 33.951 324 135 12 31 281 219 536 1.93e-29 116
Msa0310440 MtrunA17_Chr3g0112291 32.069 290 151 10 33 281 92 376 1.38e-27 109
Msa0310440 MtrunA17_Chr4g0001761 29.283 321 147 13 30 281 20 329 2.44e-27 108
Msa0310440 MtrunA17_Chr1g0150071 27.358 318 154 10 32 281 22 330 9.88e-27 107
Msa0310440 MtrunA17_Chr8g0389921 34.016 244 132 7 63 281 196 435 1.09e-26 108
Msa0310440 MtrunA17_Chr1g0150781 33.086 269 128 9 58 281 283 544 2.27e-26 108
Msa0310440 MtrunA17_Chr3g0136811 27.358 318 154 10 32 281 22 330 8.53e-26 104
Msa0310440 MtrunA17_Chr1g0158781 33.574 277 156 8 29 281 108 380 1.01e-25 104
Msa0310440 MtrunA17_Chr3g0135961 31.203 266 137 7 58 281 279 540 1.01e-25 106
Msa0310440 MtrunA17_Chr5g0435721 33.766 231 132 6 33 246 192 418 5.26e-24 100
Msa0310440 MtrunA17_Chr5g0396731 31.282 195 117 4 65 246 107 297 6.82e-22 93.6
Msa0310440 MtrunA17_Chr7g0272061 38.547 179 83 9 125 278 183 359 1.96e-21 92.8
Msa0310440 MtrunA17_Chr1g0192421 35.349 215 109 11 91 280 141 350 5.89e-21 90.9
Msa0310440 MtrunA17_Chr4g0067711 36.275 204 103 10 102 280 151 352 8.19e-21 90.9
Msa0310440 MtrunA17_Chr7g0263281 28.276 290 129 10 58 278 64 343 2.25e-20 89.7
Msa0310440 MtrunA17_Chr2g0295401 37.500 216 105 12 90 280 143 353 6.49e-20 88.2
Msa0310440 MtrunA17_Chr1g0209451 28.030 264 159 10 40 278 38 295 9.71e-20 88.2
Msa0310440 MtrunA17_Chr3g0127091 27.090 299 156 11 31 277 58 346 1.27e-19 87.8
Msa0310440 MtrunA17_Chr6g0486661 28.016 257 141 10 65 278 124 379 2.06e-19 88.2
Msa0310440 MtrunA17_Chr1g0181901 29.911 224 114 7 65 247 76 297 2.37e-19 87.0
Msa0310440 MtrunA17_Chr3g0089711 28.528 326 153 12 23 278 29 344 4.73e-19 85.9
Msa0310440 MtrunA17_Chr3g0089721 28.528 326 153 12 23 278 29 344 4.88e-19 85.9
Msa0310440 MtrunA17_Chr8g0371321 28.000 275 129 10 68 278 82 351 6.01e-19 85.9
Msa0310440 MtrunA17_Chr1g0213351 28.223 287 134 11 58 278 72 352 1.15e-18 84.7
Msa0310440 MtrunA17_Chr7g0256771 28.063 253 121 10 40 246 53 290 1.29e-18 84.0
Msa0310440 MtrunA17_Chr1g0153671 29.185 233 141 9 65 278 65 292 2.23e-18 84.3
Msa0310440 MtrunA17_Chr1g0211251 29.204 226 113 8 65 247 81 302 4.04e-18 83.6
Msa0310440 MtrunA17_Chr7g0221691 38.122 181 90 10 102 264 208 384 1.08e-17 82.4
Msa0310440 MtrunA17_Chr7g0250061 28.315 279 129 12 65 278 76 348 1.86e-17 81.6
Msa0310440 MtrunA17_Chr7g0225501 31.513 238 137 11 63 278 84 317 5.83e-17 80.5
Msa0310440 MtrunA17_Chr4g0004971 40.000 160 77 7 124 266 165 322 6.27e-17 79.7
Msa0310440 MtrunA17_Chr2g0324061 36.420 162 85 7 102 246 162 322 1.09e-16 79.7
Msa0310440 MtrunA17_Chr4g0068971 38.333 120 59 3 169 278 115 229 1.43e-16 77.8
Msa0310440 MtrunA17_Chr7g0271971 27.660 235 146 8 63 278 66 295 2.87e-16 78.2
Msa0310440 MtrunA17_Chr7g0249421 36.420 162 85 7 102 246 183 343 4.93e-16 77.8
Msa0310440 MtrunA17_Chr1g0192471 25.818 275 177 9 25 278 27 295 8.22e-16 77.0
Msa0310440 MtrunA17_Chr7g0238501 27.778 252 131 9 68 270 266 515 1.05e-15 77.0
Msa0310440 MtrunA17_Chr7g0243571 35.669 157 85 6 125 266 188 343 1.25e-15 76.6
Msa0310440 MtrunA17_Chr3g0133801 27.778 234 143 9 65 278 66 293 1.34e-15 76.3
Msa0310440 MtrunA17_Chr7g0256751 26.101 318 153 13 33 280 42 347 7.34e-15 74.3
Msa0310440 MtrunA17_Chr2g0294291 27.237 257 141 10 57 270 66 319 1.46e-14 73.2
Msa0310440 MtrunA17_Chr1g0207571 25.407 307 158 12 36 278 36 335 1.25e-13 70.5
Msa0310440 MtrunA17_Chr1g0151781 29.289 239 128 10 53 251 55 292 7.11e-13 67.8
Msa0310440 MtrunA17_Chr8g0342431 36.913 149 76 6 132 264 203 349 8.19e-13 68.2
Msa0310440 MtrunA17_Chr4g0063051 28.947 228 138 11 60 270 55 275 1.19e-12 67.0
Msa0310440 MtrunA17_Chr1g0175381 25.436 287 152 13 33 270 53 326 2.37e-11 63.5
Msa0310440 MtrunA17_Chr3g0089691 27.984 243 106 9 101 278 20 258 7.69e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0310440 AT1G34750.2 70.567 282 82 1 1 281 1 282 9.30e-146 410
Msa0310440 AT1G34750.1 70.567 282 82 1 1 281 1 282 9.30e-146 410
Msa0310440 AT1G34750.3 70.567 282 82 1 1 281 1 282 9.30e-146 410
Msa0310440 AT1G22280.1 68.327 281 89 0 1 281 1 281 4.55e-139 393
Msa0310440 AT1G22280.3 66.899 287 89 1 1 281 1 287 1.72e-136 387
Msa0310440 AT1G78200.1 62.324 284 103 3 1 281 1 283 2.04e-125 358
Msa0310440 AT1G78200.3 62.324 284 103 3 1 281 1 283 2.04e-125 358
Msa0310440 AT1G78200.2 62.324 284 103 3 1 281 1 283 2.04e-125 358
Msa0310440 AT1G34750.4 68.016 247 76 2 1 246 1 245 5.02e-119 341
Msa0310440 AT1G78200.5 67.227 238 75 2 47 281 1 238 1.35e-112 325
Msa0310440 AT1G78200.4 67.227 238 75 2 47 281 1 238 1.35e-112 325
Msa0310440 AT2G20630.1 59.023 266 106 2 19 281 14 279 1.47e-110 321
Msa0310440 AT4G28400.1 58.427 267 106 2 19 280 16 282 9.59e-109 317
Msa0310440 AT3G15260.2 60.517 271 105 2 13 281 19 289 2.39e-108 316
Msa0310440 AT3G15260.1 60.517 271 105 2 13 281 19 289 2.39e-108 316
Msa0310440 AT4G28400.3 59.073 259 105 1 23 280 2 260 2.52e-108 315
Msa0310440 AT2G20630.2 58.491 265 107 2 19 280 14 278 1.27e-107 314
Msa0310440 AT4G28400.2 57.589 224 90 2 19 237 16 239 1.26e-87 262
Msa0310440 AT2G34740.1 53.200 250 111 3 34 280 88 334 5.93e-84 255
Msa0310440 AT1G22280.2 68.108 185 59 0 1 185 1 185 9.30e-83 247
Msa0310440 AT5G10740.1 47.547 265 126 6 22 280 22 279 6.46e-73 228
Msa0310440 AT5G10740.2 47.547 265 126 6 22 280 22 279 6.46e-73 228
Msa0310440 AT5G24940.1 46.241 266 130 6 21 280 21 279 8.10e-71 225
Msa0310440 AT2G34740.3 54.106 207 89 3 34 237 84 287 1.59e-70 220
Msa0310440 AT2G34740.2 54.106 207 89 3 34 237 102 305 1.62e-70 221
Msa0310440 AT4G31750.1 45.420 262 136 5 22 280 22 279 4.25e-70 219
Msa0310440 AT4G31750.3 45.420 262 136 5 22 280 22 279 4.25e-70 219
Msa0310440 AT4G31750.2 45.420 262 136 5 22 280 22 279 4.56e-70 219
Msa0310440 AT5G53140.1 43.511 262 141 5 23 281 91 348 5.40e-65 209
Msa0310440 AT5G53140.2 43.511 262 141 5 23 281 91 348 5.40e-65 209
Msa0310440 AT1G43900.1 41.502 253 140 6 32 281 122 369 3.30e-59 193
Msa0310440 AT3G51470.2 34.661 251 143 7 45 280 39 283 1.56e-39 140
Msa0310440 AT2G33700.1 35.448 268 146 8 33 280 84 344 5.79e-39 140
Msa0310440 AT3G51470.1 34.661 251 143 7 45 280 83 327 7.79e-39 139
Msa0310440 AT2G30020.1 40.400 250 130 11 45 281 150 393 1.09e-38 140
Msa0310440 AT3G62260.3 38.494 239 120 8 64 280 26 259 3.47e-38 136
Msa0310440 AT3G62260.1 38.494 239 120 8 64 280 115 348 2.69e-37 136
Msa0310440 AT3G62260.2 38.494 239 120 8 64 280 116 349 2.98e-37 136
Msa0310440 AT1G07160.1 39.442 251 130 9 45 280 133 376 4.71e-37 135
Msa0310440 AT2G40180.1 36.653 251 138 8 47 281 141 386 3.01e-36 133
Msa0310440 AT2G25620.1 36.325 234 124 6 64 278 127 354 4.21e-36 133
Msa0310440 AT2G25620.2 36.325 234 124 6 64 278 127 354 4.21e-36 133
Msa0310440 AT1G48040.1 34.201 269 136 10 45 280 89 349 3.62e-33 125
Msa0310440 AT3G17250.1 33.607 244 130 6 63 280 156 393 5.97e-33 125
Msa0310440 AT1G67820.2 35.841 226 131 8 47 265 97 315 2.19e-31 120
Msa0310440 AT1G67820.1 35.841 226 131 8 47 265 133 351 6.57e-31 120
Msa0310440 AT5G57050.2 34.109 258 124 9 65 281 120 372 8.40e-30 116
Msa0310440 AT5G57050.1 34.109 258 124 9 65 281 160 412 9.24e-30 116
Msa0310440 AT4G26080.1 33.115 305 142 13 31 281 127 423 2.41e-29 115
Msa0310440 AT2G40860.4 33.333 249 150 6 46 281 306 551 3.47e-27 110
Msa0310440 AT2G40860.1 33.333 249 150 6 46 281 404 649 3.62e-27 110
Msa0310440 AT1G07430.1 34.034 238 129 8 33 246 120 353 3.75e-27 109
Msa0310440 AT3G11410.1 33.032 221 113 6 56 247 128 342 4.16e-27 108
Msa0310440 AT5G51760.2 33.333 318 148 11 24 281 69 382 6.50e-27 108
Msa0310440 AT2G29380.1 32.031 256 127 8 66 281 113 361 6.87e-27 107
Msa0310440 AT1G18030.1 31.481 270 147 11 45 281 85 349 7.04e-27 107
Msa0310440 AT5G51760.1 33.333 318 148 11 24 281 99 412 8.00e-27 108
Msa0310440 AT2G25070.1 27.445 317 155 11 32 281 22 330 6.45e-26 105
Msa0310440 AT2G25070.2 27.445 317 155 11 32 281 22 330 6.45e-26 105
Msa0310440 AT1G72770.4 42.222 180 78 5 125 281 326 502 8.87e-26 106
Msa0310440 AT1G72770.1 42.222 180 78 5 125 281 326 502 8.87e-26 106
Msa0310440 AT1G72770.3 42.222 180 78 5 125 281 326 502 8.87e-26 106
Msa0310440 AT2G40860.2 33.051 236 142 6 46 268 404 636 8.14e-25 104
Msa0310440 AT4G31860.1 27.044 318 155 11 32 281 22 330 1.83e-24 101
Msa0310440 AT5G59220.1 32.340 235 134 6 33 246 111 341 3.00e-24 101
Msa0310440 AT3G23360.1 28.627 255 146 6 31 280 36 259 4.36e-24 98.2
Msa0310440 AT1G17550.1 31.227 269 130 7 65 281 237 502 6.59e-24 101
Msa0310440 AT1G17550.2 31.227 269 130 7 65 281 237 502 6.59e-24 101
Msa0310440 AT4G31860.3 28.102 274 129 10 32 246 22 286 6.96e-23 95.9
Msa0310440 AT4G27800.1 28.852 305 153 11 27 277 51 345 7.42e-23 97.1
Msa0310440 AT3G16800.4 40.104 192 88 10 115 281 65 254 1.58e-22 94.0
Msa0310440 AT3G16800.6 40.104 192 88 10 115 281 65 254 1.58e-22 94.0
Msa0310440 AT1G18030.2 32.579 221 116 9 45 237 85 300 2.29e-22 94.7
Msa0310440 AT3G05640.2 37.255 204 101 9 102 280 148 349 2.32e-22 95.5
Msa0310440 AT3G05640.1 37.255 204 101 9 102 280 148 349 2.32e-22 95.5
Msa0310440 AT3G05640.3 37.255 204 101 9 102 280 148 349 2.32e-22 95.5
Msa0310440 AT3G16800.2 40.104 192 88 10 115 281 160 349 3.74e-22 94.7
Msa0310440 AT3G16800.1 40.104 192 88 10 115 281 160 349 3.74e-22 94.7
Msa0310440 AT3G16800.3 40.104 192 88 10 115 281 160 349 3.74e-22 94.7
Msa0310440 AT5G27930.1 36.634 202 101 10 102 278 153 352 4.86e-21 92.0
Msa0310440 AT5G27930.2 36.634 202 101 10 102 278 153 352 4.86e-21 92.0
Msa0310440 AT4G31860.2 27.757 263 122 10 32 235 22 275 4.98e-20 87.8
Msa0310440 AT4G08260.1 30.165 242 129 9 47 281 1 209 5.79e-20 86.3
Msa0310440 AT3G12620.2 30.851 282 130 11 59 278 77 355 5.87e-20 89.0
Msa0310440 AT3G12620.1 30.851 282 130 11 59 278 77 355 5.87e-20 89.0
Msa0310440 AT1G68410.4 26.562 256 162 9 43 278 51 300 1.08e-19 88.6
Msa0310440 AT1G68410.3 26.562 256 162 9 43 278 51 300 1.08e-19 88.6
Msa0310440 AT1G68410.1 26.562 256 162 9 43 278 51 300 1.08e-19 88.6
Msa0310440 AT1G68410.2 26.562 256 162 9 43 278 51 300 1.08e-19 88.6
Msa0310440 AT4G27800.2 27.336 289 149 11 27 265 51 328 2.03e-19 87.0
Msa0310440 AT3G51370.1 29.084 251 131 8 65 270 79 327 2.16e-19 87.4
Msa0310440 AT3G51370.3 29.084 251 131 8 65 270 79 327 2.16e-19 87.4
Msa0310440 AT2G20050.1 28.740 254 140 10 65 278 143 395 2.26e-19 88.6
Msa0310440 AT4G27800.3 27.985 268 133 10 27 244 51 308 2.31e-19 86.7
Msa0310440 AT2G20050.2 28.740 254 140 10 65 278 143 395 2.51e-19 88.2
Msa0310440 AT1G09160.2 27.239 268 168 9 32 278 34 295 3.59e-19 87.0
Msa0310440 AT1G09160.1 27.239 268 168 9 32 278 34 295 3.59e-19 87.0
Msa0310440 AT1G47380.1 26.953 256 161 9 43 278 42 291 1.22e-18 85.5
Msa0310440 AT5G66080.1 29.084 251 125 9 68 270 85 330 3.26e-18 84.0
Msa0310440 AT3G27140.1 30.992 242 107 8 47 281 1 189 4.17e-18 82.0
Msa0310440 AT4G38520.2 28.571 280 136 11 63 280 78 355 4.37e-18 84.0
Msa0310440 AT4G38520.1 28.571 280 136 11 63 280 78 355 4.37e-18 84.0
Msa0310440 AT5G06750.3 29.110 292 139 10 43 272 50 335 2.59e-17 81.6
Msa0310440 AT5G06750.1 29.110 292 139 10 43 272 50 335 2.59e-17 81.6
Msa0310440 AT5G01700.1 29.839 248 134 10 58 266 21 267 2.62e-17 80.9
Msa0310440 AT3G55050.1 25.979 281 145 8 59 278 78 356 2.78e-17 81.3
Msa0310440 AT3G55050.2 25.979 281 145 8 59 278 78 356 2.78e-17 81.3
Msa0310440 AT5G01700.2 29.839 248 134 10 58 266 70 316 3.37e-17 81.3
Msa0310440 AT1G79630.2 34.066 182 102 7 102 266 128 308 8.90e-17 80.1
Msa0310440 AT1G79630.7 34.066 182 102 7 102 266 128 308 8.90e-17 80.1
Msa0310440 AT1G79630.1 34.066 182 102 7 102 266 198 378 1.09e-16 80.1
Msa0310440 AT3G02750.2 35.556 180 92 8 90 246 174 352 1.17e-16 80.1
Msa0310440 AT3G02750.1 35.556 180 92 8 90 246 174 352 1.17e-16 80.1
Msa0310440 AT1G79630.8 34.066 182 102 7 102 266 194 374 1.23e-16 80.1
Msa0310440 AT1G79630.6 34.066 182 102 7 102 266 88 268 1.51e-16 79.3
Msa0310440 AT1G79630.3 34.066 182 102 7 102 266 88 268 1.51e-16 79.3
Msa0310440 AT1G79630.4 34.066 182 102 7 102 266 88 268 1.51e-16 79.3
Msa0310440 AT1G79630.5 34.066 182 102 7 102 266 88 268 1.51e-16 79.3
Msa0310440 AT5G02760.1 28.125 288 141 12 21 247 9 291 1.93e-16 79.0
Msa0310440 AT1G16220.1 36.478 159 84 6 124 266 198 355 6.27e-16 77.8
Msa0310440 AT3G17090.1 27.113 284 143 10 57 278 75 356 1.92e-15 75.9
Msa0310440 AT3G51370.2 30.660 212 101 7 103 270 33 242 1.97e-15 75.1
Msa0310440 AT5G36250.1 34.973 183 99 8 102 266 183 363 1.00e-14 74.3
Msa0310440 AT3G17090.2 26.724 232 128 7 57 247 75 305 1.66e-14 72.8
Msa0310440 AT5G66080.2 30.189 212 102 7 103 270 17 226 2.74e-14 71.6
Msa0310440 AT2G40860.3 30.769 195 120 5 46 228 404 595 9.17e-14 71.6
Msa0310440 AT2G05050.1 39.394 99 54 2 189 281 92 190 3.33e-13 67.4
Msa0310440 AT5G26010.1 27.935 247 130 10 37 246 47 282 4.41e-13 68.9
Msa0310440 AT4G33920.1 27.148 291 134 13 58 278 54 336 6.08e-13 68.6
Msa0310440 AT5G57050.3 32.680 153 83 7 65 202 160 307 6.34e-13 68.2
Msa0310440 AT4G32950.1 30.638 235 129 11 58 264 65 293 6.50e-13 68.2
Msa0310440 AT5G19280.1 25.784 287 171 12 28 280 299 577 7.40e-13 68.9
Msa0310440 AT5G59220.2 30.000 190 108 6 33 201 111 296 2.56e-12 66.2
Msa0310440 AT5G27930.3 36.424 151 76 8 102 234 153 301 9.54e-12 64.7
Msa0310440 AT5G36250.2 32.487 197 99 9 102 266 183 377 1.25e-11 65.1
Msa0310440 AT5G19280.2 25.253 297 170 13 28 280 299 587 1.73e-11 64.7

Find 83 sgRNAs with CRISPR-Local

Find 222 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTTCTCACAGTCCTGATTT+GGG 0.253672 2_4:+5232164 Msa0310440:CDS
TCTTTCTCACAGTCCTGATT+TGG 0.254999 2_4:+5232163 Msa0310440:CDS
TGAGAGAGAGTTGAATTAAA+TGG 0.263700 2_4:+5231026 Msa0310440:exon
ATGAGAGGTAAGATCTTTAT+AGG 0.272783 2_4:-5230829 None:intergenic
TAATAAATAATCAGAAATTA+TGG 0.279094 2_4:+5232219 Msa0310440:CDS
ATGAAATCAATTTATCAGTT+TGG 0.285612 2_4:+5233758 Msa0310440:three_prime_UTR
GGTAAGATCTTTATAGGAAG+TGG 0.290720 2_4:-5230823 None:intergenic
TGAAGCTCTTCGATTCAATT+AGG 0.301274 2_4:+5233829 Msa0310440:three_prime_UTR
CTTCAAGGCCCTCTTAAGTA+TGG 0.331523 2_4:+5231806 Msa0310440:CDS
CTACCAAAGAGCTTATTAAT+AGG 0.340721 2_4:-5230702 None:intergenic
ATGATGCGTCTTTCAGTATT+GGG 0.345245 2_4:-5232317 None:intergenic
TTTCCTATTAATAAGCTCTT+TGG 0.346720 2_4:+5230699 Msa0310440:five_prime_UTR
CTTGCAGTTGCTCGGGCATT+CGG 0.354027 2_4:+5233354 Msa0310440:CDS
GGAAAGAATTTGTAAGCATC+TGG 0.358340 2_4:-5230681 None:intergenic
TTTATATTGACAATGGGAAT+TGG 0.367194 2_4:+5233957 Msa0310440:three_prime_UTR
ATGGCTGCAAAACACAAATA+AGG 0.374341 2_4:-5230593 None:intergenic
CCAACTCTCGCCCGTTGAAC+TGG 0.390268 2_4:-5231892 None:intergenic
GGCAAAGGCAAGAGCCTTCA+AGG 0.390525 2_4:+5231791 Msa0310440:CDS
GATGATGCGTCTTTCAGTAT+TGG 0.404290 2_4:-5232318 None:intergenic
GATCCTCCACGCCCCAAATC+AGG 0.407496 2_4:-5232176 None:intergenic
CATACTTAAGAGGGCCTTGA+AGG 0.416633 2_4:-5231805 None:intergenic
TACTTGCTAGTGATGGTCTA+TGG 0.418352 2_4:+5233457 Msa0310440:CDS
GCTTCGATGATCGAACTGTT+TGG 0.423468 2_4:-5232116 None:intergenic
CACAGTCCTGATTTGGGGCG+TGG 0.426527 2_4:+5232170 Msa0310440:CDS
GTATCAAGAGCTCAGTATCT+TGG 0.427375 2_4:-5233437 None:intergenic
TTTCTGATTATTTATTAGAA+TGG 0.428271 2_4:-5232213 None:intergenic
CAGATCGCAAGTGTGACTTC+AGG 0.437990 2_4:-5233386 None:intergenic
AGACGCATCATCGAGAATAA+AGG 0.440708 2_4:+5232329 Msa0310440:CDS
AGCTTATGAGGCAACTGATC+AGG 0.443548 2_4:+5232136 Msa0310440:CDS
AAGCAAAAGCCATACTTAAG+AGG 0.443765 2_4:-5231815 None:intergenic
CCTTCAAGATATTAGAAAAG+AGG 0.445161 2_4:-5231976 None:intergenic
CGAAAATCGTCCAGTTCAAC+GGG 0.446953 2_4:+5231882 Msa0310440:CDS
CAGTTCAACGGGCGAGAGTT+GGG 0.457156 2_4:+5231893 Msa0310440:CDS
TTGCAATATCAACTGCCTCT+TGG 0.459676 2_4:-5233598 None:intergenic
TGCTATATTTGATGGACACT+CGG 0.459898 2_4:+5231922 Msa0310440:CDS
GGACGTTCTTTATGCGGCTC+TGG 0.465744 2_4:+5231770 Msa0310440:CDS
TGGACAGCTTGCAGTTGCTC+GGG 0.484799 2_4:+5233347 Msa0310440:CDS
GTAAGATCTTTATAGGAAGT+GGG 0.489143 2_4:-5230822 None:intergenic
ATGGACAGCTTGCAGTTGCT+CGG 0.494076 2_4:+5233346 Msa0310440:CDS
GGTGATGTTGCAAGAGTGAA+TGG 0.495091 2_4:+5233327 Msa0310440:intron
CCAGTTCAACGGGCGAGAGT+TGG 0.504156 2_4:+5231892 Msa0310440:CDS
GTATCTTGGTCAATATCGTC+GGG 0.509507 2_4:-5233423 None:intergenic
GCGAAAATCGTCCAGTTCAA+CGG 0.511342 2_4:+5231881 Msa0310440:CDS
AGTATCTTGGTCAATATCGT+CGG 0.513251 2_4:-5233424 None:intergenic
TTCGATCATCGAAGCTTATG+AGG 0.516217 2_4:+5232124 Msa0310440:CDS
AGGCATTGAAAAGAGAGAGC+AGG 0.521221 2_4:+5233666 Msa0310440:CDS
AAAGATAAAAGATCCACAAA+AGG 0.522450 2_4:+5233622 Msa0310440:CDS
GTTGCGGAAATGAAGAGAAA+AGG 0.523684 2_4:-5230777 None:intergenic
TCTTTATGCGGCTCTGGCAA+AGG 0.527519 2_4:+5231776 Msa0310440:CDS
TTTCACTGAATTGATCATCT+GGG 0.532697 2_4:+5230894 Msa0310440:five_prime_UTR
GGCATTGAAAAGAGAGAGCA+GGG 0.537795 2_4:+5233667 Msa0310440:CDS
TTATAGGAAGTGGGTGGTCA+CGG 0.537871 2_4:-5230813 None:intergenic
AAATTATGGATAGCAAATGT+TGG 0.546553 2_4:+5232233 Msa0310440:CDS
CTCTTGATACTTGCTAGTGA+TGG 0.551258 2_4:+5233450 Msa0310440:CDS
GCTATATTTGATGGACACTC+GGG 0.552095 2_4:+5231923 Msa0310440:CDS
AGAAATCAAGAAATACTGAA+TGG 0.557197 2_4:-5233857 None:intergenic
AGCTTGAGTAACTTGATCCT+CGG 0.566493 2_4:+5233997 Msa0310440:three_prime_UTR
GTTGGAGGACGTTCTTTATG+CGG 0.569210 2_4:+5231764 Msa0310440:CDS
GCAAAACACAAATAAGGAAG+AGG 0.569566 2_4:-5230587 None:intergenic
TTTCTCACAGTCCTGATTTG+GGG 0.570400 2_4:+5232165 Msa0310440:CDS
AGCAAAAGCCATACTTAAGA+GGG 0.571518 2_4:-5231814 None:intergenic
CGAGCAGTTCTGTCGAGGAA+AGG 0.583792 2_4:+5232263 Msa0310440:CDS
AGCTCTTCGATTCAATTAGG+TGG 0.590001 2_4:+5233832 Msa0310440:three_prime_UTR
TTGCTTCAACTCTTCCCGAA+AGG 0.594951 2_4:+5231059 Msa0310440:CDS
TGCTAGTGATGGTCTATGGA+AGG 0.596717 2_4:+5233461 Msa0310440:CDS
GGTTTATTAAAGAACCTGGA+AGG 0.597472 2_4:-5232367 None:intergenic
TTTGGGGCGTGGAGGATCGA+CGG 0.597691 2_4:+5232181 Msa0310440:CDS
TCATCTGAGTGAAAATTCAG+AGG 0.600464 2_4:+5230929 Msa0310440:five_prime_UTR
CTTGAAGCGAACTACAATGC+AGG 0.608420 2_4:-5233699 None:intergenic
TCTTCATTTCCGCAACAGAA+CGG 0.618247 2_4:+5230784 Msa0310440:five_prime_UTR
AGTCCTGATTTGGGGCGTGG+AGG 0.620509 2_4:+5232173 Msa0310440:CDS
ATTCACGAGCAGTTCTGTCG+AGG 0.639885 2_4:+5232258 Msa0310440:CDS
TCAGGTAATGGCAAACCAAG+AGG 0.643063 2_4:+5233583 Msa0310440:intron
AGTCGCTTATATTATAGCCG+AGG 0.645750 2_4:-5234014 None:intergenic
AGCTAAACAACTAACTGCAG+AGG 0.651227 2_4:+5233646 Msa0310440:CDS
CGTGAAAGTCCTCCATTGTG+TGG 0.653511 2_4:-5231856 None:intergenic
GACACAAGACCACACAATGG+AGG 0.665307 2_4:+5231847 Msa0310440:CDS
AGTTCTGTCGAGGAAAGGTG+TGG 0.670672 2_4:+5232268 Msa0310440:CDS
AGCAGTGTGAAGATGAACAT+TGG 0.673038 2_4:+5233932 Msa0310440:three_prime_UTR
CGCATCATCGAGAATAAAGG+CGG 0.678594 2_4:+5232332 Msa0310440:CDS
TGGGAATTGGAATACATGAG+TGG 0.686678 2_4:+5233970 Msa0310440:three_prime_UTR
AGATCTTTATAGGAAGTGGG+TGG 0.704685 2_4:-5230819 None:intergenic
AGGGACACAAGACCACACAA+TGG 0.718438 2_4:+5231844 Msa0310440:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAGTTTAAATTTTTTGAT+TGG + chr2_4:5230861-5230880 Msa0310440:five_prime_UTR 10.0%
!! TAATAAATAATCAGAAATTA+TGG + chr2_4:5232219-5232238 Msa0310440:CDS 10.0%
!!! TAATTACAAAAAATTGTTTT+GGG - chr2_4:5232944-5232963 None:intergenic 10.0%
!!! ACTTTTATTTTGTGTTTTTT+TGG + chr2_4:5231095-5231114 Msa0310440:intron 15.0%
!! AGCTATTAAAAACTAAATTT+GGG - chr2_4:5231224-5231243 None:intergenic 15.0%
!! AAGCTATTAAAAACTAAATT+TGG - chr2_4:5231225-5231244 None:intergenic 15.0%
!! AACAACCAAAATATAATATA+TGG - chr2_4:5231590-5231609 None:intergenic 15.0%
!! TTTCTGATTATTTATTAGAA+TGG - chr2_4:5232216-5232235 None:intergenic 15.0%
!!! CTAATTACAAAAAATTGTTT+TGG - chr2_4:5232945-5232964 None:intergenic 15.0%
!!! CAAAACAATTTTTTGTAATT+AGG + chr2_4:5232943-5232962 Msa0310440:intron 15.0%
!!! CCTACTTATAAATATATTTT+AGG - chr2_4:5233105-5233124 None:intergenic 15.0%
!! CCTAAAATATATTTATAAGT+AGG + chr2_4:5233102-5233121 Msa0310440:intron 15.0%
!! TTATCATAAGTTCAAAATTT+AGG - chr2_4:5233171-5233190 None:intergenic 15.0%
!!! TAAATTTTGAACTTATGATA+AGG + chr2_4:5233170-5233189 Msa0310440:intron 15.0%
!!! AATAATTTTAGAAACAATTG+CGG - chr2_4:5233518-5233537 None:intergenic 15.0%
!! AAAAATGTAAAAAGTCAACA+AGG + chr2_4:5230560-5230579 Msa0310440:five_prime_UTR 20.0%
!! AAAATGTAAAAAGTCAACAA+GGG + chr2_4:5230561-5230580 Msa0310440:five_prime_UTR 20.0%
!!! AAAGTCTTTTGTTTTTTCAA+TGG - chr2_4:5230615-5230634 None:intergenic 20.0%
!! TACAACATATAACAAAACAA+TGG - chr2_4:5232014-5232033 None:intergenic 20.0%
!! TTATATGTTGTAATGTCTTT+CGG + chr2_4:5232022-5232041 Msa0310440:intron 20.0%
!!! AACAATTTTTTGTAATTAGG+AGG + chr2_4:5232946-5232965 Msa0310440:intron 20.0%
!! TATGATAAGGTTTATTTAGA+TGG + chr2_4:5233183-5233202 Msa0310440:intron 20.0%
!! GACATTAGAAGATAAATTTA+AGG - chr2_4:5233496-5233515 None:intergenic 20.0%
!!! TCAATTGATTACATATTTTC+AGG + chr2_4:5233565-5233584 Msa0310440:intron 20.0%
!! ATGAAATCAATTTATCAGTT+TGG + chr2_4:5233758-5233777 Msa0310440:three_prime_UTR 20.0%
!!! ATTGGTTTTTATATTGACAA+TGG + chr2_4:5233950-5233969 Msa0310440:three_prime_UTR 20.0%
!!! TTGGTTTTTATATTGACAAT+GGG + chr2_4:5233951-5233970 Msa0310440:three_prime_UTR 20.0%
! TTTCCTATTAATAAGCTCTT+TGG + chr2_4:5230699-5230718 Msa0310440:five_prime_UTR 25.0%
!! TTTGTTCTTTCTTTTGTCTT+TGG + chr2_4:5231138-5231157 Msa0310440:intron 25.0%
!!! TTTCTTTTGTCTTTGGAATA+TGG + chr2_4:5231145-5231164 Msa0310440:intron 25.0%
! TAATTCTAACGTTGAGAAAT+TGG - chr2_4:5231178-5231197 None:intergenic 25.0%
!! TCAACGTTAGAATTAAGTTT+TGG + chr2_4:5231183-5231202 Msa0310440:intron 25.0%
!!! AATTAAGTTTTGGTGATTTG+AGG + chr2_4:5231193-5231212 Msa0310440:intron 25.0%
! TGATGAACAAAATTGAAACT+TGG + chr2_4:5231248-5231267 Msa0310440:intron 25.0%
! GATGAACAAAATTGAAACTT+GGG + chr2_4:5231249-5231268 Msa0310440:intron 25.0%
!!! TTATATTTTGGTTGTTGTAC+TGG + chr2_4:5231594-5231613 Msa0310440:intron 25.0%
!! GTTTAGTCTTCAACATAAAA+TGG - chr2_4:5231627-5231646 None:intergenic 25.0%
! CATAACATAAATGTCAATGT+TGG - chr2_4:5231649-5231668 None:intergenic 25.0%
! ACATTGACATTTATGTTATG+TGG + chr2_4:5231649-5231668 Msa0310440:intron 25.0%
!!! TGCTAGTAATTTGTTAAAAG+TGG + chr2_4:5231694-5231713 Msa0310440:intron 25.0%
!!! ATTTTAACTTGCAACGTTTT+AGG + chr2_4:5231739-5231758 Msa0310440:intron 25.0%
!!! TTTTAACTTGCAACGTTTTA+GGG + chr2_4:5231740-5231759 Msa0310440:intron 25.0%
!!! AAAGAGGTGTTTTTGTAAAT+AGG - chr2_4:5231963-5231982 None:intergenic 25.0%
! AAAATCTCACAAGTTATGAT+TGG + chr2_4:5232066-5232085 Msa0310440:intron 25.0%
! AAATTATGGATAGCAAATGT+TGG + chr2_4:5232233-5232252 Msa0310440:CDS 25.0%
! TTAAGGTTTATTAAAGAACC+TGG - chr2_4:5232374-5232393 None:intergenic 25.0%
! CAATATCTCATAAGACATTA+AGG - chr2_4:5232391-5232410 None:intergenic 25.0%
!! TACATCTTTTATGGTTCATA+AGG + chr2_4:5232592-5232611 Msa0310440:intron 25.0%
! GATTCTACACTTTATGTTAT+AGG - chr2_4:5232656-5232675 None:intergenic 25.0%
! AATTAGAGCATGATTAAACT+GGG - chr2_4:5232720-5232739 None:intergenic 25.0%
! AAATTAGAGCATGATTAAAC+TGG - chr2_4:5232721-5232740 None:intergenic 25.0%
! GCATATAGTTATATCAAGTT+GGG - chr2_4:5232811-5232830 None:intergenic 25.0%
! AGCATATAGTTATATCAAGT+TGG - chr2_4:5232812-5232831 None:intergenic 25.0%
! AACTATATGCTAGAGAATAA+AGG + chr2_4:5232821-5232840 Msa0310440:intron 25.0%
! ACTATATGCTAGAGAATAAA+GGG + chr2_4:5232822-5232841 Msa0310440:intron 25.0%
!!! GATTACATATTTTCAGGTAA+TGG + chr2_4:5233571-5233590 Msa0310440:intron 25.0%
! AAAGATAAAAGATCCACAAA+AGG + chr2_4:5233622-5233641 Msa0310440:CDS 25.0%
!! TGAATTTTACATGGGAAAAA+GGG - chr2_4:5233806-5233825 None:intergenic 25.0%
!! CTTCAAACTGAATTTTACAT+GGG - chr2_4:5233814-5233833 None:intergenic 25.0%
! AGAAATCAAGAAATACTGAA+TGG - chr2_4:5233860-5233879 None:intergenic 25.0%
! TTTATATTGACAATGGGAAT+TGG + chr2_4:5233957-5233976 Msa0310440:three_prime_UTR 25.0%
CTACCAAAGAGCTTATTAAT+AGG - chr2_4:5230705-5230724 None:intergenic 30.0%
GTAAGATCTTTATAGGAAGT+GGG - chr2_4:5230825-5230844 None:intergenic 30.0%
ATGAGAGGTAAGATCTTTAT+AGG - chr2_4:5230832-5230851 None:intergenic 30.0%
!! ACTTTTTCCTGAAAAATGAG+AGG - chr2_4:5230847-5230866 None:intergenic 30.0%
! TTTTCACTGAATTGATCATC+TGG + chr2_4:5230893-5230912 Msa0310440:five_prime_UTR 30.0%
TTTCACTGAATTGATCATCT+GGG + chr2_4:5230894-5230913 Msa0310440:five_prime_UTR 30.0%
TGAGAGAGAGTTGAATTAAA+TGG + chr2_4:5231026-5231045 Msa0310440:exon 30.0%
TAAAAACATCATCACCTTTC+GGG - chr2_4:5231076-5231095 None:intergenic 30.0%
GTAAAAACATCATCACCTTT+CGG - chr2_4:5231077-5231096 None:intergenic 30.0%
!! GGGATTGTTTTATGTTTTTG+TGG + chr2_4:5231269-5231288 Msa0310440:intron 30.0%
! GCTATGTTTTACTTTCATCT+TGG + chr2_4:5231557-5231576 Msa0310440:intron 30.0%
! GTTTTACTTTCATCTTGGAT+TGG + chr2_4:5231562-5231581 Msa0310440:intron 30.0%
! TGGCGCCATATATTATATTT+TGG + chr2_4:5231582-5231601 Msa0310440:intron 30.0%
!! GGACTTTTTGCTATATTTGA+TGG + chr2_4:5231914-5231933 Msa0310440:CDS 30.0%
CCTTCAAGATATTAGAAAAG+AGG - chr2_4:5231979-5231998 None:intergenic 30.0%
! CCTCTTTTCTAATATCTTGA+AGG + chr2_4:5231976-5231995 Msa0310440:CDS 30.0%
! TCACAAGTTATGATTGGTTA+TGG + chr2_4:5232072-5232091 Msa0310440:intron 30.0%
ATTCATCTTGTCATTCTATG+AGG + chr2_4:5232434-5232453 Msa0310440:intron 30.0%
AGCATAAGACTTCAATTTGA+TGG - chr2_4:5232497-5232516 None:intergenic 30.0%
TTATTAGCAAATACATCCAC+AGG - chr2_4:5232554-5232573 None:intergenic 30.0%
! CATTCTTCGTACATCTTTTA+TGG + chr2_4:5232583-5232602 Msa0310440:intron 30.0%
GCTTCACTTGTTTAGAAAAT+AGG + chr2_4:5232755-5232774 Msa0310440:intron 30.0%
! GAAGTTATGGTTATTGGTTA+GGG - chr2_4:5232880-5232899 None:intergenic 30.0%
TCAATAAGAGTAGGAAGTTA+TGG - chr2_4:5232893-5232912 None:intergenic 30.0%
ACATTAACAAGCCAAAACTT+GGG - chr2_4:5233227-5233246 None:intergenic 30.0%
TACATTAACAAGCCAAAACT+TGG - chr2_4:5233228-5233247 None:intergenic 30.0%
! TTGTTAATGTAGAGGTACAT+TGG + chr2_4:5233237-5233256 Msa0310440:intron 30.0%
! CATTGATGTTAAATCCGTTT+TGG + chr2_4:5233264-5233283 Msa0310440:intron 30.0%
! CTGAATTTTACATGGGAAAA+AGG - chr2_4:5233807-5233826 None:intergenic 30.0%
! GCTTCAAACTGAATTTTACA+TGG - chr2_4:5233815-5233834 None:intergenic 30.0%
TATTGTGTGTGTGTGTATAT+TGG + chr2_4:5234047-5234066 Msa0310440:three_prime_UTR 30.0%
GCAAAACACAAATAAGGAAG+AGG - chr2_4:5230590-5230609 None:intergenic 35.0%
ATGGCTGCAAAACACAAATA+AGG - chr2_4:5230596-5230615 None:intergenic 35.0%
GGAAAGAATTTGTAAGCATC+TGG - chr2_4:5230684-5230703 None:intergenic 35.0%
GGTAAGATCTTTATAGGAAG+TGG - chr2_4:5230826-5230845 None:intergenic 35.0%
!! GATCTTACCTCTCATTTTTC+AGG + chr2_4:5230837-5230856 Msa0310440:five_prime_UTR 35.0%
TCATCTGAGTGAAAATTCAG+AGG + chr2_4:5230929-5230948 Msa0310440:five_prime_UTR 35.0%
!!! TTCAGAGGTTTTGAAAGTTG+TGG + chr2_4:5230944-5230963 Msa0310440:five_prime_UTR 35.0%
AGATATGAGCTTATGTAGCA+CGG + chr2_4:5231346-5231365 Msa0310440:intron 35.0%
ATGAAGCATAAACACAGACA+CGG - chr2_4:5231485-5231504 None:intergenic 35.0%
TGTCTGTGTTTATGCTTCAT+AGG + chr2_4:5231485-5231504 Msa0310440:intron 35.0%
AGCAAAAGCCATACTTAAGA+GGG - chr2_4:5231817-5231836 None:intergenic 35.0%
AAGCAAAAGCCATACTTAAG+AGG - chr2_4:5231818-5231837 None:intergenic 35.0%
! TATGGCTTTTGCTTAGTTGA+AGG + chr2_4:5231824-5231843 Msa0310440:CDS 35.0%
! ATGGCTTTTGCTTAGTTGAA+GGG + chr2_4:5231825-5231844 Msa0310440:CDS 35.0%
! TGCTATATTTGATGGACACT+CGG + chr2_4:5231922-5231941 Msa0310440:CDS 35.0%
!! AGGTGTTTTTGTAAATAGGC+AGG - chr2_4:5231959-5231978 None:intergenic 35.0%
! AAGTTATGATTGGTTATGGC+AGG + chr2_4:5232076-5232095 Msa0310440:intron 35.0%
ATGATGCGTCTTTCAGTATT+GGG - chr2_4:5232320-5232339 None:intergenic 35.0%
GGTTTATTAAAGAACCTGGA+AGG - chr2_4:5232370-5232389 None:intergenic 35.0%
! GGTTTTCATCAACAATGGAA+AGG - chr2_4:5232533-5232552 None:intergenic 35.0%
ACTAAACATGGATTCCTACA+TGG - chr2_4:5232697-5232716 None:intergenic 35.0%
GATTAAACTGGGACTAAACA+TGG - chr2_4:5232709-5232728 None:intergenic 35.0%
TTCTAAACAAGTGAAGCTCA+GGG - chr2_4:5232752-5232771 None:intergenic 35.0%
TTTCTAAACAAGTGAAGCTC+AGG - chr2_4:5232753-5232772 None:intergenic 35.0%
ACTTGTTTAGAAAATAGGCC+TGG + chr2_4:5232760-5232779 Msa0310440:intron 35.0%
AGGCAACACATAATAAATCC+AGG - chr2_4:5232781-5232800 None:intergenic 35.0%
!!! ATATCAAGTTGGGCTTTTTG+AGG - chr2_4:5232801-5232820 None:intergenic 35.0%
! GGAAGTTATGGTTATTGGTT+AGG - chr2_4:5232881-5232900 None:intergenic 35.0%
GAGTAGGAAGTTATGGTTAT+TGG - chr2_4:5232886-5232905 None:intergenic 35.0%
GGACTCAAATCAATAAGAGT+AGG - chr2_4:5232902-5232921 None:intergenic 35.0%
!! TATTGATTTGAGTCCTGCAT+TGG + chr2_4:5232907-5232926 Msa0310440:intron 35.0%
!! ATTCACAAAACCGACTTGTA+AGG - chr2_4:5232981-5233000 None:intergenic 35.0%
! TAGTCCAATAGATCTAAGGT+AGG - chr2_4:5233022-5233041 None:intergenic 35.0%
CAGATAGTCCAATAGATCTA+AGG - chr2_4:5233026-5233045 None:intergenic 35.0%
ACTATCTGTTATCAACCACT+TGG + chr2_4:5233038-5233057 Msa0310440:intron 35.0%
CAAAACTTGGGTTTGATGTT+AGG - chr2_4:5233215-5233234 None:intergenic 35.0%
! CTAACATCAAACCCAAGTTT+TGG + chr2_4:5233213-5233232 Msa0310440:intron 35.0%
!!! GTTTTGGCTTGTTAATGTAG+AGG + chr2_4:5233229-5233248 Msa0310440:intron 35.0%
TTCACAACACTCAACCAAAA+CGG - chr2_4:5233281-5233300 None:intergenic 35.0%
TCACTTGTGGATGTTATATC+AGG + chr2_4:5233306-5233325 Msa0310440:intron 35.0%
AGTATCTTGGTCAATATCGT+CGG - chr2_4:5233427-5233446 None:intergenic 35.0%
TGAAGCTCTTCGATTCAATT+AGG + chr2_4:5233829-5233848 Msa0310440:three_prime_UTR 35.0%
GTTGCGGAAATGAAGAGAAA+AGG - chr2_4:5230780-5230799 None:intergenic 40.0%
TCTTCATTTCCGCAACAGAA+CGG + chr2_4:5230784-5230803 Msa0310440:five_prime_UTR 40.0%
AGATCTTTATAGGAAGTGGG+TGG - chr2_4:5230822-5230841 None:intergenic 40.0%
GACACACCTTCAATCTAAAG+TGG - chr2_4:5231372-5231391 None:intergenic 40.0%
! CTTTAGATTGAAGGTGTGTC+TGG + chr2_4:5231372-5231391 Msa0310440:intron 40.0%
CACTATGACACTGATACATG+AGG + chr2_4:5231407-5231426 Msa0310440:intron 40.0%
ATGAGGCACATTCAAACTAC+TGG + chr2_4:5231424-5231443 Msa0310440:intron 40.0%
CACATTCAAACTACTGGTGT+CGG + chr2_4:5231430-5231449 Msa0310440:intron 40.0%
!! TTTTGGTTGTTGTACTGGTG+TGG + chr2_4:5231599-5231618 Msa0310440:intron 40.0%
! GCTATATTTGATGGACACTC+GGG + chr2_4:5231923-5231942 Msa0310440:CDS 40.0%
! GGTTATGGCAGGAGAATTTT+TGG + chr2_4:5232087-5232106 Msa0310440:intron 40.0%
TTCGATCATCGAAGCTTATG+AGG + chr2_4:5232124-5232143 Msa0310440:CDS 40.0%
TCTTTCTCACAGTCCTGATT+TGG + chr2_4:5232163-5232182 Msa0310440:CDS 40.0%
CTTTCTCACAGTCCTGATTT+GGG + chr2_4:5232164-5232183 Msa0310440:CDS 40.0%
! TTTCTCACAGTCCTGATTTG+GGG + chr2_4:5232165-5232184 Msa0310440:CDS 40.0%
GATGATGCGTCTTTCAGTAT+TGG - chr2_4:5232321-5232340 None:intergenic 40.0%
AGACGCATCATCGAGAATAA+AGG + chr2_4:5232329-5232348 Msa0310440:CDS 40.0%
! CCACAGGTTTTCATCAACAA+TGG - chr2_4:5232538-5232557 None:intergenic 40.0%
! CCATTGTTGATGAAAACCTG+TGG + chr2_4:5232535-5232554 Msa0310440:intron 40.0%
AGAATCGTCTTGAGAAGTCA+TGG + chr2_4:5232670-5232689 Msa0310440:intron 40.0%
GTCCTGCATTGGATGTTATA+AGG + chr2_4:5232918-5232937 Msa0310440:intron 40.0%
! AATAGATCTAAGGTAGGCTC+TGG - chr2_4:5233016-5233035 None:intergenic 40.0%
AGAGCCTACCTTAGATCTAT+TGG + chr2_4:5233015-5233034 Msa0310440:intron 40.0%
GTTGTGAACTTTCTCACTTG+TGG + chr2_4:5233293-5233312 Msa0310440:intron 40.0%
GTATCTTGGTCAATATCGTC+GGG - chr2_4:5233426-5233445 None:intergenic 40.0%
GTATCAAGAGCTCAGTATCT+TGG - chr2_4:5233440-5233459 None:intergenic 40.0%
! CTCTTGATACTTGCTAGTGA+TGG + chr2_4:5233450-5233469 Msa0310440:CDS 40.0%
! TACTTGCTAGTGATGGTCTA+TGG + chr2_4:5233457-5233476 Msa0310440:CDS 40.0%
TTGCAATATCAACTGCCTCT+TGG - chr2_4:5233601-5233620 None:intergenic 40.0%
! AGTTGTTTAGCTGCCTTTTG+TGG - chr2_4:5233638-5233657 None:intergenic 40.0%
AGCTAAACAACTAACTGCAG+AGG + chr2_4:5233646-5233665 Msa0310440:CDS 40.0%
AGCTCTTCGATTCAATTAGG+TGG + chr2_4:5233832-5233851 Msa0310440:three_prime_UTR 40.0%
AGCAGTGTGAAGATGAACAT+TGG + chr2_4:5233932-5233951 Msa0310440:three_prime_UTR 40.0%
TGGGAATTGGAATACATGAG+TGG + chr2_4:5233970-5233989 Msa0310440:three_prime_UTR 40.0%
AGCTTGAGTAACTTGATCCT+CGG + chr2_4:5233997-5234016 Msa0310440:three_prime_UTR 40.0%
AGTCGCTTATATTATAGCCG+AGG - chr2_4:5234017-5234036 None:intergenic 40.0%
TTATAGGAAGTGGGTGGTCA+CGG - chr2_4:5230816-5230835 None:intergenic 45.0%
TTGCTTCAACTCTTCCCGAA+AGG + chr2_4:5231059-5231078 Msa0310440:CDS 45.0%
CAGCAACTTCATAACAGTCG+TGG + chr2_4:5231296-5231315 Msa0310440:intron 45.0%
ATAAACACAGACACGGACAC+CGG - chr2_4:5231478-5231497 None:intergenic 45.0%
!!! CTTGCAACGTTTTAGGGTGT+TGG + chr2_4:5231746-5231765 Msa0310440:intron 45.0%
GTTGGAGGACGTTCTTTATG+CGG + chr2_4:5231764-5231783 Msa0310440:CDS 45.0%
CATACTTAAGAGGGCCTTGA+AGG - chr2_4:5231808-5231827 None:intergenic 45.0%
CTTCAAGGCCCTCTTAAGTA+TGG + chr2_4:5231806-5231825 Msa0310440:CDS 45.0%
GCGAAAATCGTCCAGTTCAA+CGG + chr2_4:5231881-5231900 Msa0310440:CDS 45.0%
CGAAAATCGTCCAGTTCAAC+GGG + chr2_4:5231882-5231901 Msa0310440:CDS 45.0%
GCTTCGATGATCGAACTGTT+TGG - chr2_4:5232119-5232138 None:intergenic 45.0%
AGCTTATGAGGCAACTGATC+AGG + chr2_4:5232136-5232155 Msa0310440:CDS 45.0%
CGCATCATCGAGAATAAAGG+CGG + chr2_4:5232332-5232351 Msa0310440:CDS 45.0%
!! GGTTTTGTCTCAAACCTTCC+AGG + chr2_4:5232353-5232372 Msa0310440:CDS 45.0%
TGAGAAGTCATGGACCATGT+AGG + chr2_4:5232680-5232699 Msa0310440:intron 45.0%
! GGCCTTATAACATCCAATGC+AGG - chr2_4:5232923-5232942 None:intergenic 45.0%
GTATCTCGCACCTTACAAGT+CGG + chr2_4:5232968-5232987 Msa0310440:intron 45.0%
! ATATCAGAAGCGTGACCAAG+TGG - chr2_4:5233056-5233075 None:intergenic 45.0%
GGTGATGTTGCAAGAGTGAA+TGG + chr2_4:5233327-5233346 Msa0310440:intron 45.0%
! ATATCGTCGGGTTTTACGTC+TGG - chr2_4:5233414-5233433 None:intergenic 45.0%
TGCTAGTGATGGTCTATGGA+AGG + chr2_4:5233461-5233480 Msa0310440:CDS 45.0%
! TCAGGTAATGGCAAACCAAG+AGG + chr2_4:5233583-5233602 Msa0310440:intron 45.0%
!! AGGCATTGAAAAGAGAGAGC+AGG + chr2_4:5233666-5233685 Msa0310440:CDS 45.0%
! GGCATTGAAAAGAGAGAGCA+GGG + chr2_4:5233667-5233686 Msa0310440:CDS 45.0%
CTTGAAGCGAACTACAATGC+AGG - chr2_4:5233702-5233721 None:intergenic 45.0%
! CGGATTTTGCCGTTCTGTTG+CGG - chr2_4:5230796-5230815 None:intergenic 50.0%
! GCACGGCCACTTTAGATTGA+AGG + chr2_4:5231363-5231382 Msa0310440:intron 50.0%
GCGTGTCTGTGTAAGTGACA+TGG + chr2_4:5231452-5231471 Msa0310440:intron 50.0%
GTCTGTGTAAGTGACATGGC+CGG + chr2_4:5231456-5231475 Msa0310440:intron 50.0%
!!! GCAACGTTTTAGGGTGTTGG+AGG + chr2_4:5231749-5231768 Msa0310440:intron 50.0%
! TCTTTATGCGGCTCTGGCAA+AGG + chr2_4:5231776-5231795 Msa0310440:CDS 50.0%
AGGGACACAAGACCACACAA+TGG + chr2_4:5231844-5231863 Msa0310440:CDS 50.0%
GACACAAGACCACACAATGG+AGG + chr2_4:5231847-5231866 Msa0310440:CDS 50.0%
CGTGAAAGTCCTCCATTGTG+TGG - chr2_4:5231859-5231878 None:intergenic 50.0%
ATTCACGAGCAGTTCTGTCG+AGG + chr2_4:5232258-5232277 Msa0310440:CDS 50.0%
AGTTCTGTCGAGGAAAGGTG+TGG + chr2_4:5232268-5232287 Msa0310440:CDS 50.0%
ATGGACAGCTTGCAGTTGCT+CGG + chr2_4:5233346-5233365 Msa0310440:CDS 50.0%
CAGATCGCAAGTGTGACTTC+AGG - chr2_4:5233389-5233408 None:intergenic 50.0%
ACAGTCGTGGATGCTGTGTG+AGG + chr2_4:5231309-5231328 Msa0310440:intron 55.0%
! TTGAAGGTGTGTCTGGTGCG+CGG + chr2_4:5231379-5231398 Msa0310440:intron 55.0%
GGACGTTCTTTATGCGGCTC+TGG + chr2_4:5231770-5231789 Msa0310440:CDS 55.0%
GGCAAAGGCAAGAGCCTTCA+AGG + chr2_4:5231791-5231810 Msa0310440:CDS 55.0%
CAGTTCAACGGGCGAGAGTT+GGG + chr2_4:5231893-5231912 Msa0310440:CDS 55.0%
CGAGCAGTTCTGTCGAGGAA+AGG + chr2_4:5232263-5232282 Msa0310440:CDS 55.0%
TGGACAGCTTGCAGTTGCTC+GGG + chr2_4:5233347-5233366 Msa0310440:CDS 55.0%
CTTGCAGTTGCTCGGGCATT+CGG + chr2_4:5233354-5233373 Msa0310440:CDS 55.0%
CCAACTCTCGCCCGTTGAAC+TGG - chr2_4:5231895-5231914 None:intergenic 60.0%
CCAGTTCAACGGGCGAGAGT+TGG + chr2_4:5231892-5231911 Msa0310440:CDS 60.0%
!! CACAGTCCTGATTTGGGGCG+TGG + chr2_4:5232170-5232189 Msa0310440:CDS 60.0%
!! AGTCCTGATTTGGGGCGTGG+AGG + chr2_4:5232173-5232192 Msa0310440:CDS 60.0%
GATCCTCCACGCCCCAAATC+AGG - chr2_4:5232179-5232198 None:intergenic 60.0%
! TTTGGGGCGTGGAGGATCGA+CGG + chr2_4:5232181-5232200 Msa0310440:CDS 60.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 5230527 5234096 5230527 ID=Msa0310440;Name=Msa0310440
chr2_4 mRNA 5230527 5234096 5230527 ID=Msa0310440-mRNA-1;Parent=Msa0310440;Name=Msa0310440-mRNA-1;_AED=0.25;_eAED=0.25;_QI=518|1|1|1|1|1|5|372|281
chr2_4 exon 5230527 5231080 5230527 ID=Msa0310440-mRNA-1:exon:1862;Parent=Msa0310440-mRNA-1
chr2_4 exon 5231761 5232000 5231761 ID=Msa0310440-mRNA-1:exon:1863;Parent=Msa0310440-mRNA-1
chr2_4 exon 5232098 5232374 5232098 ID=Msa0310440-mRNA-1:exon:1864;Parent=Msa0310440-mRNA-1
chr2_4 exon 5233328 5233482 5233328 ID=Msa0310440-mRNA-1:exon:1865;Parent=Msa0310440-mRNA-1
chr2_4 exon 5233587 5234096 5233587 ID=Msa0310440-mRNA-1:exon:1866;Parent=Msa0310440-mRNA-1
chr2_4 five_prime_UTR 5230527 5231044 5230527 ID=Msa0310440-mRNA-1:five_prime_utr;Parent=Msa0310440-mRNA-1
chr2_4 CDS 5231045 5231080 5231045 ID=Msa0310440-mRNA-1:cds;Parent=Msa0310440-mRNA-1
chr2_4 CDS 5231761 5232000 5231761 ID=Msa0310440-mRNA-1:cds;Parent=Msa0310440-mRNA-1
chr2_4 CDS 5232098 5232374 5232098 ID=Msa0310440-mRNA-1:cds;Parent=Msa0310440-mRNA-1
chr2_4 CDS 5233328 5233482 5233328 ID=Msa0310440-mRNA-1:cds;Parent=Msa0310440-mRNA-1
chr2_4 CDS 5233587 5233724 5233587 ID=Msa0310440-mRNA-1:cds;Parent=Msa0310440-mRNA-1
chr2_4 three_prime_UTR 5233725 5234096 5233725 ID=Msa0310440-mRNA-1:three_prime_utr;Parent=Msa0310440-mRNA-1
Gene Sequence

>Msa0310440

ATGGATAGTCTCTGTTGCTTCAACTCTTCCCGAAAGGGTGTTGGAGGACGTTCTTTATGCGGCTCTGGCAAAGGCAAGAGCCTTCAAGGCCCTCTTAAGTATGGCTTTTGCTTAGTTGAAGGGACACAAGACCACACAATGGAGGACTTTCACGTTGCGAAAATCGTCCAGTTCAACGGGCGAGAGTTGGGACTTTTTGCTATATTTGATGGACACTCGGGTGACACTGTGCCTGCCTATTTACAAAAACACCTCTTTTCTAATATCTTGAAGGAAGAGAATTTTTGGACTGATCCAAACAGTTCGATCATCGAAGCTTATGAGGCAACTGATCAGGCTATTCTTTCTCACAGTCCTGATTTGGGGCGTGGAGGATCGACGGCAGTGACTGCCATTCTAATAAATAATCAGAAATTATGGATAGCAAATGTTGGAGATTCACGAGCAGTTCTGTCGAGGAAAGGTGTGGCTATACAGATGAGTATTGATCACGAGCCCAATACTGAAAGACGCATCATCGAGAATAAAGGCGGTTTTGTCTCAAACCTTCCAGGTGATGTTGCAAGAGTGAATGGACAGCTTGCAGTTGCTCGGGCATTCGGAGATAGAAACCTGAAGTCACACTTGCGATCTGATCCAGACGTAAAACCCGACGATATTGACCAAGATACTGAGCTCTTGATACTTGCTAGTGATGGTCTATGGAAGGTAATGGCAAACCAAGAGGCAGTTGATATTGCAATAAAGATAAAAGATCCACAAAAGGCAGCTAAACAACTAACTGCAGAGGCATTGAAAAGAGAGAGCAGGGATGACATTTCCTGCATTGTAGTTCGCTTCAAGTGA
Protein sequence

>Msa0310440

MDSLCCFNSSRKGVGGRSLCGSGKGKSLQGPLKYGFCLVEGTQDHTMEDFHVAKIVQFNGRELGLFAIFDGHSGDTVPAYLQKHLFSNILKEENFWTDPNSSIIEAYEATDQAILSHSPDLGRGGSTAVTAILINNQKLWIANVGDSRAVLSRKGVAIQMSIDHEPNTERRIIENKGGFVSNLPGDVARVNGQLAVARAFGDRNLKSHLRSDPDVKPDDIDQDTELLILASDGLWKVMANQEAVDIAIKIKDPQKAAKQLTAEALKRESRDDISCIVVRFK*