AlfalfaGEDB | Msa1453130

M. sativa cultivar zhongmu-4 / Msa1453130

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1453130	G7L1P5	98.621	145	2	0	1	145	1	145	1.05e-99	291

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000220	Msa1453130	0.850866	1.287315e-60	-8.615850e-47
Msa0001040	Msa1453130	0.820780	5.724681e-53	-8.615850e-47
Msa0001510	Msa1453130	0.900653	5.908716e-78	-8.615850e-47
Msa0001540	Msa1453130	0.851126	1.087429e-60	-8.615850e-47
Msa0002060	Msa1453130	0.835661	1.473418e-56	-8.615850e-47
Msa0002690	Msa1453130	0.895123	1.292539e-75	-8.615850e-47
Msa0002700	Msa1453130	0.883939	2.933476e-71	-8.615850e-47
Msa0003340	Msa1453130	0.816949	4.248553e-52	-8.615850e-47
Msa0004180	Msa1453130	0.891514	3.706586e-74	-8.615850e-47
Msa0005190	Msa1453130	0.808151	3.564863e-50	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1453130	MtrunA17_Chr7g0263571	98.621	145	2	0	1	145	1	145	2.03e-103	291

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1453130	AT3G53470.2	68.750	96	30	0	48	143	40	135	1.17e-43	140
Msa1453130	AT3G53470.1	71.111	90	26	0	54	143	45	134	5.50e-42	136

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Find 51 sgRNAs with CRISPR-Local

Find 180 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGAGAATAACAAATCCG+AGG	0.655714	tig0034497:-2997	None:intergenic
AAAGATCAAACCTCAAATTC+TGG	0.301138	tig0034497:+2755	Msa1453130:CDS
AACATACTACAAATTTGAAG+GGG	0.613458	tig0034497:-1651	None:intergenic
AAGAAGAGGATGATAAAGTT+TGG	0.290458	tig0034497:-1615	None:intergenic
AATAGAACAGGAATAGAAAC+AGG	0.428545	tig0034497:-2939	None:intergenic
AATATGTGAGCTGGAATCCT+AGG	0.492726	tig0034497:+2683	Msa1453130:CDS
AATTGTGTCAAGGACTCGAG+TGG	0.625018	tig0034497:-2969	None:intergenic
AATTTGTAGTATGTTCCAAA+AGG	0.463127	tig0034497:+1659	Msa1453130:CDS
ACGATGGGATATAAAAGTTG+TGG	0.530720	tig0034497:-1578	None:intergenic
ACTTGTTGATTGGTGCTTGA+AGG	0.468811	tig0034497:-2840	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AGAAGAGGATGATAAAGTTT+GGG	-	tig0034497:1617-1636	None:intergenic	30.0%
AAGAAGAGGATGATAAAGTT+TGG	-	tig0034497:1618-1637	None:intergenic	30.0%
TACAAATTTGAAGGGGAAAT+AGG	-	tig0034497:1647-1666	None:intergenic	30.0%
GGAACATACTACAAATTTGA+AGG	-	tig0034497:1656-1675	None:intergenic	30.0%
CAAAATACAGTGTTTAAGAG+AGG	+	tig0034497:2125-2144	Msa1453130:intron	30.0%
AAATACAGTGTTTAAGAGAG+GGG	+	tig0034497:2127-2146	Msa1453130:intron	30.0%
TTTCCTTCCTAGAAAGTAAT+CGG	+	tig0034497:2157-2176	Msa1453130:intron	30.0%
TTCCAGCTCACATATTTATT+TGG	-	tig0034497:2679-2698	None:intergenic	30.0%
AAAGATCAAACCTCAAATTC+TGG	+	tig0034497:2755-2774	Msa1453130:CDS	30.0%
AATAGAACAGGAATAGAAAC+AGG	-	tig0034497:2942-2961	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
tig0034497	gene	1454	3097	1454	ID=Msa1453130;Name=Msa1453130
tig0034497	mRNA	1454	3097	1454	ID=Msa1453130-mRNA-1;Parent=Msa1453130;Name=Msa1453130-mRNA-1;_AED=0.12;_eAED=0.12;_QI=96\|1\|1\|1\|0\|0.5\|2\|233\|145
tig0034497	exon	1454	1715	1454	ID=Msa1453130-mRNA-1:exon:19021;Parent=Msa1453130-mRNA-1
tig0034497	exon	2593	3097	2593	ID=Msa1453130-mRNA-1:exon:19022;Parent=Msa1453130-mRNA-1
tig0034497	five_prime_UTR	1454	1549	1454	ID=Msa1453130-mRNA-1:five_prime_utr;Parent=Msa1453130-mRNA-1
tig0034497	CDS	1550	1715	1550	ID=Msa1453130-mRNA-1:cds;Parent=Msa1453130-mRNA-1
tig0034497	CDS	2593	2864	2593	ID=Msa1453130-mRNA-1:cds;Parent=Msa1453130-mRNA-1
tig0034497	three_prime_UTR	2865	3097	2865	ID=Msa1453130-mRNA-1:three_prime_utr;Parent=Msa1453130-mRNA-1

Gene Sequence

>Msa1453130

ATGGATATTCATAGACACATTTCAATACCCACAACTTTTATATCCCATCGTTTCTCTTCACCAACCCAAACTTTATCATCCTCTTCTTTCCATTCCTATTTCCCCTTCAAATTTGTAGTATGTTCCAAAAGGGACGATCAATCTCAAAGAAACGCCGATAACAATGGTGATAAATCATCAACGGATTGGGACAAGGCATGGTCAAACTTCAAGAAGCAGGGAAAAAAAACTCTCTTCTCAAATTTTTCTCCAAATAAATATGTGAGCTGGAATCCTAGGCGTTCAGATTTTCCACTTTCTGAAGAAGTTGATCCTATCAAGAGAACAGAAAGATCAAACCTCAAATTCTGGACTAGTCCTACTTTTACTCTTGGTGGGGCACTTATTATTCTCATAGTTCTTTTGTTGTACACCCTTCAAGCACCAATCAACAAGTAA

Protein sequence

>Msa1453130

MDIHRHISIPTTFISHRFSSPTQTLSSSSFHSYFPFKFVVCSKRDDQSQRNADNNGDKSSTDWDKAWSNFKKQGKKTLFSNFSPNKYVSWNPRRSDFPLSEEVDPIKRTERSNLKFWTSPTFTLGGALIILIVLLLYTLQAPINK*