AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0237960


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0237960 MtrunA17_Chr2g0287391 84.375 192 17 2 1 192 1 179 3.02e-101 290
Msa0237960 MtrunA17_Chr2g0286581 49.215 191 85 5 3 193 13 191 7.12e-45 147
Msa0237960 MtrunA17_Chr2g0287311 44.286 210 92 5 1 193 11 212 4.10e-37 128
Msa0237960 MtrunA17_Chr2g0287401 43.005 193 77 4 1 193 1 160 1.31e-33 117
Msa0237960 MtrunA17_Chr2g0287351 57.609 92 36 1 3 94 2 90 9.15e-27 102
Msa0237960 MtrunA17_Chr4g0019961 38.095 189 107 3 4 192 3 181 8.69e-24 93.2
Msa0237960 MtrunA17_Chr2g0287321 36.410 195 83 6 4 193 13 171 1.37e-23 92.0
Msa0237960 MtrunA17_Chr2g0287331 54.545 77 32 1 7 83 3 76 1.65e-20 82.4
Msa0237960 MtrunA17_Chr1g0156951 31.381 239 97 7 3 191 2 223 6.85e-20 83.6
Msa0237960 MtrunA17_Chr2g0287301 35.870 184 78 5 3 186 13 156 1.29e-19 81.3
Msa0237960 MtrunA17_Chr2g0287421 53.247 77 33 1 1 77 1 74 2.17e-18 78.6
Msa0237960 MtrunA17_Chr2g0287371 50.633 79 36 1 1 79 4 79 2.04e-17 77.4
Msa0237960 MtrunA17_Chr1g0170131 49.367 79 33 1 3 81 2 73 2.19e-16 74.3
Msa0237960 MtrunA17_Chr5g0420551 26.222 225 115 5 16 193 8 228 1.53e-14 69.7
Msa0237960 MtrunA17_Chr2g0288061 33.880 183 110 3 12 189 11 187 1.75e-14 68.6
Msa0237960 MtrunA17_Chr2g0287341 60.714 56 17 2 1 56 1 51 1.14e-12 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 38 sgRNAs with CRISPR-Local

Find 39 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGTGCTTCTTAAGGAATTT+AGG 0.194647 2_2:+11207940 None:intergenic
TTCTGACGTGGATAATCTTT+TGG 0.310646 2_2:-11207656 Msa0237960:CDS
CACGGACACGGTGGTTCTTC+CGG 0.314955 2_2:-11208058 Msa0237960:CDS
ATCCTCCAGTGCTTGGGATT+GGG 0.342543 2_2:+11207579 None:intergenic
TATCCTCCAGTGCTTGGGAT+TGG 0.354112 2_2:+11207578 None:intergenic
CGAATTGAAACGGGTGTTGA+TGG 0.362790 2_2:-11207686 Msa0237960:CDS
AGATCTAACCTCCAAGGCTT+TGG 0.383571 2_2:-11207893 Msa0237960:CDS
ATTTCGATTATCAGTTCTTC+CGG 0.390404 2_2:+11208039 None:intergenic
CATTATATCCTCCAGTGCTT+GGG 0.417270 2_2:+11207573 None:intergenic
TCATTATATCCTCCAGTGCT+TGG 0.419753 2_2:+11207572 None:intergenic
AGAACCACCGTGTCCGTGTT+TGG 0.425629 2_2:+11208063 None:intergenic
GAATTCCATCGTTTGCAAAC+GGG 0.440834 2_2:+11207976 None:intergenic
TTCTTCCTCCTCTTGAGGCA+TGG 0.443136 2_2:+11207825 None:intergenic
CGAATTCCATCGTTTGCAAA+CGG 0.446779 2_2:+11207975 None:intergenic
TCATTTCCAGAGTTGATTCA+AGG 0.453871 2_2:-11208017 Msa0237960:CDS
AGCTCTGAAAGTGCTTCTTA+AGG 0.482555 2_2:+11207932 None:intergenic
AGCAATGACGGCGGAAGCAA+CGG 0.488849 2_2:-11208094 None:intergenic
GCACTTTCAGAGCTTGTTCA+CGG 0.493716 2_2:-11207923 Msa0237960:CDS
GGACTGTTCCAAAGCCTTGG+AGG 0.494324 2_2:+11207885 None:intergenic
TCTTCTTCTTCCTCCTCTTG+AGG 0.507183 2_2:+11207820 None:intergenic
ATTGGACTGTTCCAAAGCCT+TGG 0.508501 2_2:+11207882 None:intergenic
AGTATTCCTTGAATCAACTC+TGG 0.529325 2_2:+11208011 None:intergenic
GCAAACCCAATCCCAAGCAC+TGG 0.551500 2_2:-11207584 Msa0237960:CDS
CGCGGCCATGCCTCAAGAGG+AGG 0.572319 2_2:-11207830 Msa0237960:CDS
CAAGGAATACTCTGCAACTG+AGG 0.574910 2_2:-11207999 Msa0237960:CDS
AACCCAATCCCAAGCACTGG+AGG 0.597028 2_2:-11207581 Msa0237960:CDS
AACGGCGGAGTCACCAAACA+CGG 0.597428 2_2:-11208076 Msa0237960:CDS
TGAGGCCCGTTTGCAAACGA+TGG 0.616269 2_2:-11207981 Msa0237960:CDS
TAACGCGGCCATGCCTCAAG+AGG 0.620807 2_2:-11207833 Msa0237960:CDS
ATGTTGCGATATGAAAGCAT+TGG 0.637121 2_2:+11207864 None:intergenic
AATGACGGCGGAAGCAACGG+CGG 0.640542 2_2:-11208091 Msa0237960:CDS
CATCAAAGATCTAACCTCCA+AGG 0.641038 2_2:-11207899 Msa0237960:CDS
GGAGTCACCAAACACGGACA+CGG 0.649843 2_2:-11208070 Msa0237960:CDS
GATGGATTTAGTTTCTGACG+TGG 0.707895 2_2:-11207668 Msa0237960:CDS
GTCACCAAACACGGACACGG+TGG 0.741586 2_2:-11208067 Msa0237960:CDS
CTTAACGAGAGCAATGACGG+CGG 0.742276 2_2:-11208103 None:intergenic
ATTGGGTTTGCATATCAACG+TGG 0.746293 2_2:+11207596 None:intergenic
GCAACATATTATAAATAACG+CGG 0.768241 2_2:-11207848 Msa0237960:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! GCAACATATTATAAATAACG+CGG - chr2_2:11207773-11207792 Msa0237960:CDS 25.0%
! AAGAAAAAGACGAATTGAAA+CGG - chr2_2:11207925-11207944 Msa0237960:CDS 25.0%
!! ATAATCTTTTGGTGAATCTT+CGG - chr2_2:11207976-11207995 Msa0237960:CDS 25.0%
ATTTCGATTATCAGTTCTTC+CGG + chr2_2:11207585-11207604 None:intergenic 30.0%
AAGTGCTTCTTAAGGAATTT+AGG + chr2_2:11207684-11207703 None:intergenic 30.0%
AGAAAAAGACGAATTGAAAC+GGG - chr2_2:11207926-11207945 Msa0237960:CDS 30.0%
! TCATTTCCAGAGTTGATTCA+AGG - chr2_2:11207604-11207623 Msa0237960:CDS 35.0%
AGTATTCCTTGAATCAACTC+TGG + chr2_2:11207613-11207632 None:intergenic 35.0%
! ATGTTGCGATATGAAAGCAT+TGG + chr2_2:11207760-11207779 None:intergenic 35.0%
! TTCTGACGTGGATAATCTTT+TGG - chr2_2:11207965-11207984 Msa0237960:CDS 35.0%
!! AGTGTTTTCGAAGCTTTGTA+CGG - chr2_2:11208072-11208091 Msa0237960:CDS 35.0%
GAATTCCATCGTTTGCAAAC+GGG + chr2_2:11207648-11207667 None:intergenic 40.0%
CGAATTCCATCGTTTGCAAA+CGG + chr2_2:11207649-11207668 None:intergenic 40.0%
!! AGCTCTGAAAGTGCTTCTTA+AGG + chr2_2:11207692-11207711 None:intergenic 40.0%
CATCAAAGATCTAACCTCCA+AGG - chr2_2:11207722-11207741 Msa0237960:CDS 40.0%
! GATGGATTTAGTTTCTGACG+TGG - chr2_2:11207953-11207972 Msa0237960:CDS 40.0%
ATTGGGTTTGCATATCAACG+TGG + chr2_2:11208028-11208047 None:intergenic 40.0%
CATTATATCCTCCAGTGCTT+GGG + chr2_2:11208051-11208070 None:intergenic 40.0%
TCATTATATCCTCCAGTGCT+TGG + chr2_2:11208052-11208071 None:intergenic 40.0%
CAAGGAATACTCTGCAACTG+AGG - chr2_2:11207622-11207641 Msa0237960:CDS 45.0%
! GCACTTTCAGAGCTTGTTCA+CGG - chr2_2:11207698-11207717 Msa0237960:CDS 45.0%
AGATCTAACCTCCAAGGCTT+TGG - chr2_2:11207728-11207747 Msa0237960:CDS 45.0%
ATTGGACTGTTCCAAAGCCT+TGG + chr2_2:11207742-11207761 None:intergenic 45.0%
TCTTCTTCTTCCTCCTCTTG+AGG + chr2_2:11207804-11207823 None:intergenic 45.0%
!! CGAATTGAAACGGGTGTTGA+TGG - chr2_2:11207935-11207954 Msa0237960:CDS 45.0%
TTCTTCCTCCTCTTGAGGCA+TGG + chr2_2:11207799-11207818 None:intergenic 50.0%
ATCCTCCAGTGCTTGGGATT+GGG + chr2_2:11208045-11208064 None:intergenic 50.0%
TATCCTCCAGTGCTTGGGAT+TGG + chr2_2:11208046-11208065 None:intergenic 50.0%
AACGGCGGAGTCACCAAACA+CGG - chr2_2:11207545-11207564 Msa0237960:CDS 55.0%
GGAGTCACCAAACACGGACA+CGG - chr2_2:11207551-11207570 Msa0237960:CDS 55.0%
AGAACCACCGTGTCCGTGTT+TGG + chr2_2:11207561-11207580 None:intergenic 55.0%
TGAGGCCCGTTTGCAAACGA+TGG - chr2_2:11207640-11207659 Msa0237960:CDS 55.0%
GGACTGTTCCAAAGCCTTGG+AGG + chr2_2:11207739-11207758 None:intergenic 55.0%
GCAAACCCAATCCCAAGCAC+TGG - chr2_2:11208037-11208056 Msa0237960:CDS 55.0%
!! AACCCAATCCCAAGCACTGG+AGG - chr2_2:11208040-11208059 Msa0237960:CDS 55.0%
GTCACCAAACACGGACACGG+TGG - chr2_2:11207554-11207573 Msa0237960:CDS 60.0%
CACGGACACGGTGGTTCTTC+CGG - chr2_2:11207563-11207582 Msa0237960:CDS 60.0%
TAACGCGGCCATGCCTCAAG+AGG - chr2_2:11207788-11207807 Msa0237960:CDS 60.0%
CGCGGCCATGCCTCAAGAGG+AGG - chr2_2:11207791-11207810 Msa0237960:CDS 70.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 11207531 11208112 11207531 ID=Msa0237960;Name=Msa0237960
chr2_2 mRNA 11207531 11208112 11207531 ID=Msa0237960-mRNA-1;Parent=Msa0237960;Name=Msa0237960-mRNA-1;_AED=0.46;_eAED=0.46;_QI=0|-1|0|1|-1|1|1|0|193
chr2_2 exon 11207531 11208112 11207531 ID=Msa0237960-mRNA-1:exon:4906;Parent=Msa0237960-mRNA-1
chr2_2 CDS 11207531 11208112 11207531 ID=Msa0237960-mRNA-1:cds;Parent=Msa0237960-mRNA-1
Gene Sequence

>Msa0237960

ATGACGGCGGAAGCAACGGCGGAGTCACCAAACACGGACACGGTGGTTCTTCCGGAAGAACTGATAATCGAAATCATTTCCAGAGTTGATTCAAGGAATACTCTGCAACTGAGGCCCGTTTGCAAACGATGGAATTCGCTTATTACTGATCCTAAATTCCTTAAGAAGCACTTTCAGAGCTTGTTCACGGACATCAAAGATCTAACCTCCAAGGCTTTGGAACAGTCCAATGCTTTCATATCGCAACATATTATAAATAACGCGGCCATGCCTCAAGAGGAGGAAGAAGAAGATGCTGAAGAAGAAGAAGAAGAAGAAGATGCTGCTGTTGTTGAAGAAGAAGATGTTGTTGTTGCTGCTGTTGATGAAGAAGAAGATGTTGCTGCTGTTGATGAAGAAAAAGACGAATTGAAACGGGTGTTGATGGATTTAGTTTCTGACGTGGATAATCTTTTGGTGAATCTTCGGTTTATCAAAGACAATACAGAAACTATCCACGTTGATATGCAAACCCAATCCCAAGCACTGGAGGATATAATGAAGTGTTTTCGAAGCTTTGTACGGATTTATCTCAAGTCATAA

Protein sequence

>Msa0237960

MTAEATAESPNTDTVVLPEELIIEIISRVDSRNTLQLRPVCKRWNSLITDPKFLKKHFQSLFTDIKDLTSKALEQSNAFISQHIINNAAMPQEEEEEDAEEEEEEEDAAVVEEEDVVVAAVDEEEDVAAVDEEKDELKRVLMDLVSDVDNLLVNLRFIKDNTETIHVDMQTQSQALEDIMKCFRSFVRIYLKS*