Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1244610 | XP_013445387.1 | 98.450 | 129 | 2 | 0 | 1 | 129 | 1 | 129 | 5.49e-88 | 261 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1244610 | sp|Q9FN32|YIPL7_ARATH | 69.531 | 128 | 39 | 0 | 1 | 128 | 1 | 128 | 1.00e-60 | 185 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0011170 | Msa1244610 | 0.841225 | 5.404919e-58 | -8.615850e-47 |
| Msa0011660 | Msa1244610 | -0.834174 | 3.491423e-56 | -8.615850e-47 |
| Msa0011960 | Msa1244610 | 0.808558 | 2.919772e-50 | -8.615850e-47 |
| Msa0012040 | Msa1244610 | 0.822151 | 2.761746e-53 | -8.615850e-47 |
| Msa0015390 | Msa1244610 | 0.813100 | 3.036031e-51 | -8.615850e-47 |
| Msa0017510 | Msa1244610 | 0.839554 | 1.478661e-57 | -8.615850e-47 |
| Msa0020410 | Msa1244610 | 0.803598 | 3.229982e-49 | -8.615850e-47 |
| Msa0024210 | Msa1244610 | -0.815772 | 7.790640e-52 | -8.615850e-47 |
| Msa0031340 | Msa1244610 | 0.827459 | 1.546034e-54 | -8.615850e-47 |
| Msa0035150 | Msa1244610 | 0.812586 | 3.936162e-51 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1244610 | MtrunA17_Chr8g0360291 | 98.450 | 129 | 2 | 0 | 1 | 129 | 1 | 129 | 5.05e-92 | 261 |
| Msa1244610 | MtrunA17_Chr2g0316921 | 71.094 | 128 | 37 | 0 | 1 | 128 | 1 | 128 | 5.43e-64 | 191 |
| Msa1244610 | MtrunA17_Chr4g0046851 | 57.031 | 128 | 53 | 1 | 1 | 128 | 1 | 126 | 1.58e-48 | 151 |
| Msa1244610 | MtrunA17_Chr3g0113291 | 52.344 | 128 | 61 | 0 | 1 | 128 | 24 | 151 | 6.38e-46 | 145 |
| Msa1244610 | MtrunA17_Chr7g0242231 | 57.273 | 110 | 47 | 0 | 1 | 110 | 1 | 110 | 1.94e-44 | 141 |
| Msa1244610 | MtrunA17_Chr5g0436971 | 59.091 | 110 | 45 | 0 | 1 | 110 | 1 | 110 | 4.94e-44 | 140 |
| Msa1244610 | MtrunA17_Chr2g0316891 | 70.588 | 85 | 25 | 0 | 44 | 128 | 42 | 126 | 1.57e-39 | 129 |
| Msa1244610 | MtrunA17_Chr1g0188931 | 45.361 | 97 | 53 | 0 | 12 | 108 | 8 | 104 | 1.24e-31 | 108 |
| Msa1244610 | MtrunA17_Chr3g0127211 | 47.423 | 97 | 51 | 0 | 12 | 108 | 8 | 104 | 2.00e-31 | 107 |
| Msa1244610 | MtrunA17_Chr8g0346501 | 42.857 | 98 | 56 | 0 | 9 | 106 | 5 | 102 | 2.03e-26 | 95.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1244610 | AT5G53940.1 | 69.531 | 128 | 39 | 0 | 1 | 128 | 1 | 128 | 1.02e-61 | 185 |
| Msa1244610 | AT2G40110.1 | 55.814 | 129 | 56 | 1 | 1 | 128 | 1 | 129 | 4.46e-47 | 148 |
| Msa1244610 | AT3G08990.2 | 51.562 | 128 | 62 | 0 | 1 | 128 | 1 | 128 | 1.49e-46 | 147 |
| Msa1244610 | AT3G08990.1 | 51.562 | 128 | 62 | 0 | 1 | 128 | 1 | 128 | 1.49e-46 | 147 |
| Msa1244610 | AT3G08990.3 | 51.562 | 128 | 62 | 0 | 1 | 128 | 1 | 128 | 1.49e-46 | 147 |
| Msa1244610 | AT2G40110.2 | 64.706 | 102 | 36 | 0 | 1 | 102 | 1 | 102 | 1.63e-46 | 146 |
| Msa1244610 | AT3G55890.4 | 58.095 | 105 | 44 | 0 | 1 | 105 | 1 | 105 | 7.58e-42 | 135 |
| Msa1244610 | AT3G55890.1 | 58.095 | 105 | 44 | 0 | 1 | 105 | 1 | 105 | 7.58e-42 | 135 |
| Msa1244610 | AT3G55890.3 | 59.406 | 101 | 41 | 0 | 1 | 101 | 1 | 101 | 2.43e-41 | 133 |
| Msa1244610 | AT3G55890.2 | 59.406 | 101 | 41 | 0 | 1 | 101 | 1 | 101 | 2.43e-41 | 133 |
Find 42 sgRNAs with CRISPR-Local
Find 234 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAAGCCAAAAGTACAAAGA+GGG | 0.574439 | 8_2:+33287319 | Msa1244610:CDS |
| AAAGAGATTAAGATGAACCA+GGG | 0.646469 | 8_2:-33286159 | None:intergenic |
| AAGGAAATAGCAAAACAAAT+TGG | 0.377402 | 8_2:-33287643 | None:intergenic |
| AAGTGAAGAAGAAGAGTGAG+CGG | 0.553078 | 8_2:+33286210 | Msa1244610:five_prime_UTR |
| AATAGTAATACTCCCTGCCC+TGG | 0.535884 | 8_2:+33286142 | None:intergenic |
| ACTTCCTCAAGATTCATAAA+AGG | 0.314442 | 8_2:-33286191 | None:intergenic |
| ACTTTCCCTCTTTGTACTTT+TGG | 0.194241 | 8_2:-33287324 | None:intergenic |
| AGATTAAGATGAACCAGGGC+AGG | 0.485254 | 8_2:-33286155 | None:intergenic |
| AGATTTGGCCCAGGTGGCTA+AGG | 0.481908 | 8_2:+33287813 | Msa1244610:intron |
| AGCGGTGACATCAATCAAAT+GGG | 0.480919 | 8_2:+33286228 | Msa1244610:exon |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAGAGATTAAGATGAACCA+GGG | - | chr8_2:33286162-33286181 | None:intergenic | 30.0% | |
| ACTTCCTCAAGATTCATAAA+AGG | - | chr8_2:33286194-33286213 | None:intergenic | 30.0% | |
| AAACAATAAGGGTTAACGAA+TGG | - | chr8_2:33286409-33286428 | None:intergenic | 30.0% | |
| GAGAAGAAAAGCAAACAATA+AGG | - | chr8_2:33286421-33286440 | None:intergenic | 30.0% | |
| TAAAATTGCATCTCTCTCAT+AGG | + | chr8_2:33287282-33287301 | Msa1244610:intron | 30.0% | |
| TGTGTAATTCGTGTATCAAA+AGG | + | chr8_2:33287716-33287735 | Msa1244610:three_prime_UTR | 30.0% | |
| AAAGAGATTAAGATGAACCA+GGG | - | chr8_2:33286162-33286181 | None:intergenic | 30.0% | |
| ACTTCCTCAAGATTCATAAA+AGG | - | chr8_2:33286194-33286213 | None:intergenic | 30.0% | |
| AAACAATAAGGGTTAACGAA+TGG | - | chr8_2:33286409-33286428 | None:intergenic | 30.0% | |
| GAGAAGAAAAGCAAACAATA+AGG | - | chr8_2:33286421-33286440 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_2 | gene | 33286154 | 33287894 | 33286154 | ID=Msa1244610;Name=Msa1244610 |
| chr8_2 | mRNA | 33286154 | 33287894 | 33286154 | ID=Msa1244610-mRNA-1;Parent=Msa1244610;Name=Msa1244610-mRNA-1;_AED=0.16;_eAED=0.16;_QI=92|1|1|1|0.8|0.66|6|290|129 |
| chr8_2 | exon | 33286154 | 33286341 | 33286154 | ID=Msa1244610-mRNA-1:exon:10757;Parent=Msa1244610-mRNA-1 |
| chr8_2 | exon | 33286443 | 33286486 | 33286443 | ID=Msa1244610-mRNA-1:exon:10758;Parent=Msa1244610-mRNA-1 |
| chr8_2 | exon | 33287064 | 33287172 | 33287064 | ID=Msa1244610-mRNA-1:exon:10759;Parent=Msa1244610-mRNA-1 |
| chr8_2 | exon | 33287304 | 33287359 | 33287304 | ID=Msa1244610-mRNA-1:exon:10760;Parent=Msa1244610-mRNA-1 |
| chr8_2 | exon | 33287459 | 33287764 | 33287459 | ID=Msa1244610-mRNA-1:exon:10761;Parent=Msa1244610-mRNA-1 |
| chr8_2 | exon | 33287826 | 33287894 | 33287826 | ID=Msa1244610-mRNA-1:exon:10762;Parent=Msa1244610-mRNA-1 |
| chr8_2 | five_prime_UTR | 33286154 | 33286245 | 33286154 | ID=Msa1244610-mRNA-1:five_prime_utr;Parent=Msa1244610-mRNA-1 |
| chr8_2 | CDS | 33286246 | 33286341 | 33286246 | ID=Msa1244610-mRNA-1:cds;Parent=Msa1244610-mRNA-1 |
| chr8_2 | CDS | 33286443 | 33286486 | 33286443 | ID=Msa1244610-mRNA-1:cds;Parent=Msa1244610-mRNA-1 |
| chr8_2 | CDS | 33287064 | 33287172 | 33287064 | ID=Msa1244610-mRNA-1:cds;Parent=Msa1244610-mRNA-1 |
| chr8_2 | CDS | 33287304 | 33287359 | 33287304 | ID=Msa1244610-mRNA-1:cds;Parent=Msa1244610-mRNA-1 |
| chr8_2 | CDS | 33287459 | 33287543 | 33287459 | ID=Msa1244610-mRNA-1:cds;Parent=Msa1244610-mRNA-1 |
| chr8_2 | three_prime_UTR | 33287544 | 33287764 | 33287544 | ID=Msa1244610-mRNA-1:three_prime_utr;Parent=Msa1244610-mRNA-1 |
| chr8_2 | three_prime_UTR | 33287826 | 33287894 | 33287826 | ID=Msa1244610-mRNA-1:three_prime_utr;Parent=Msa1244610-mRNA-1 |
| Gene Sequence |
>Msa1244610 ATGGGGAGAATTTTTGCAGTTGAGCTGGATGGCAGAGTTTACGGCTGCAAGTCCTGCAAAACTCACCTTGCTCTTTCCACTGATCTCATCTCCAGGGGATTTTATTGTCATAGAGGAAAAGCATACCTTTTCAATAATGTTGTGAACTTCACTAGTGGAGAAGTGGTGGAAAGAGCGATGCTTTCAGGGATGCATACTGTTGCAGATATATTTTGTTGTCGCTGCGGTCAGATAATTGGCTGGAAATACGAAACTGTGCAGGAGAAAAGCCAAAAGTACAAAGAGGGAAAGTTTGTTCTTGAAAGAGGAAGGATCGTTGATGAAATCGGTTTCTCCACTGAATTCTGTGTTGAAGGCTGTGTCAGCATGAGTGATACTGAAGATGATTAG |
| Protein sequence |
>Msa1244610 MGRIFAVELDGRVYGCKSCKTHLALSTDLISRGFYCHRGKAYLFNNVVNFTSGEVVERAMLSGMHTVADIFCCRCGQIIGWKYETVQEKSQKYKEGKFVLERGRIVDEIGFSTEFCVEGCVSMSDTEDD* |
