AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880046805.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 47 sgRNAs with CRISPR-Local

Find 57 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTCTTTGAATCATGATAAT+AGG 0.233271 8:-78457298 MsG0880046805.01.T01:exon
TCTGAGTGGCCAGGAGTTAA+TGG 0.256229 8:+78457205 None:intergenic
CAAACATGTCCATCAAAGTT+TGG 0.287284 8:+78456800 None:intergenic
TACTCTCAACCAAACTTTGA+TGG 0.301864 8:-78456809 MsG0880046805.01.T01:three_prime_UTR
GAATTACGTGAAGGTGAAAA+TGG 0.315246 8:-78456884 MsG0880046805.01.T01:exon
TCACAATATGGAGCTTCATT+TGG 0.317095 8:-78457384 MsG0880046805.01.T01:five_prime_UTR
CTCTCTACACAATCACAATA+TGG 0.343546 8:-78457396 MsG0880046805.01.T01:five_prime_UTR
GAGAGAAAGAGTGGGAAGGT+TGG 0.357245 8:+78457231 None:intergenic
CACAATATGGAGCTTCATTT+GGG 0.385933 8:-78457383 MsG0880046805.01.T01:five_prime_UTR
ATCAAGACAAAGAAGGTGAT+TGG 0.388129 8:-78456621 MsG0880046805.01.T01:three_prime_UTR
TTCTTCCTCCTACAATTAAC+CGG 0.390508 8:+78456976 None:intergenic
TTCTTGCTCAAGATATTTCA+TGG 0.399762 8:-78456597 MsG0880046805.01.T01:three_prime_UTR
GATGGCCTCCGGTTAATTGT+AGG 0.427386 8:-78456984 MsG0880046805.01.T01:CDS
GATCACGTGGTCCCCATTGA+TGG 0.439591 8:-78456925 MsG0880046805.01.T01:CDS
CTGAGTGGCCAGGAGTTAAT+GGG 0.451365 8:+78457206 None:intergenic
GATGATGAAGAAGCTCTTGT+TGG 0.454535 8:-78457006 MsG0880046805.01.T01:CDS
TAATGGGAGGAGAGAAAGAG+TGG 0.454609 8:+78457222 None:intergenic
TCTCTCCTCCCATTAACTCC+TGG 0.458165 8:-78457214 MsG0880046805.01.T01:CDS
AGAAAGAGTGGGAAGGTTGG+TGG 0.462033 8:+78457234 None:intergenic
ATGGTTCTCATGATAATCAT+AGG 0.480012 8:-78456906 MsG0880046805.01.T01:CDS
ATTATCATGAGAACCATCAA+TGG 0.488136 8:+78456912 None:intergenic
AGCTCTTGTTGGATGGCCTC+CGG 0.493311 8:-78456995 MsG0880046805.01.T01:CDS
TGATCAACAACAGTATGAAC+TGG 0.501567 8:-78456647 MsG0880046805.01.T01:three_prime_UTR
TTATCATGAGAACCATCAAT+GGG 0.508335 8:+78456913 None:intergenic
ATTACTGGTGATGGTGGTAG+TGG 0.526533 8:+78457144 None:intergenic
AATAATATAATTACTGGTGA+TGG 0.527200 8:+78457135 None:intergenic
CGGACGAGAATTATTATAAG+TGG 0.529450 8:+78457349 None:intergenic
AATATAATTACTGGTGATGG+TGG 0.543697 8:+78457138 None:intergenic
CTGGTTTATCAAGACAAAGA+AGG 0.557412 8:-78456628 MsG0880046805.01.T01:three_prime_UTR
GGTTGAGAGTATGAAATGAA+TGG 0.558722 8:+78456821 None:intergenic
TCATGATCATCTGAGTGGCC+AGG 0.559365 8:+78457196 None:intergenic
GGAGGAGAGAAAGAGTGGGA+AGG 0.576479 8:+78457227 None:intergenic
AATGGGAGGAGAGAAAGAGT+GGG 0.579242 8:+78457223 None:intergenic
ATGAAGAAGCTCTTGTTGGA+TGG 0.580018 8:-78457002 MsG0880046805.01.T01:CDS
TATCATGATTCAAAGAGAAG+TGG 0.584987 8:+78457303 None:intergenic
TACGTGAAGGTGAAAATGGA+AGG 0.594315 8:-78456880 MsG0880046805.01.T01:exon
GGCCTCCGGTTAATTGTAGG+AGG 0.613414 8:-78456981 MsG0880046805.01.T01:CDS
AGGTGTAGGAATAGCTCGAA+AGG 0.621460 8:-78456860 MsG0880046805.01.T01:three_prime_UTR
TTAGTAGCAGCAATATCGTA+CGG 0.636596 8:+78457329 None:intergenic
TTCCTCCTACAATTAACCGG+AGG 0.665193 8:+78456979 None:intergenic
AAGGTGAAAATGGAAGGTGT+AGG 0.668997 8:-78456874 MsG0880046805.01.T01:three_prime_UTR
TAATCATAGGAATTACGTGA+AGG 0.675805 8:-78456893 MsG0880046805.01.T01:exon
AGTGGCCAGGAGTTAATGGG+AGG 0.697461 8:+78457209 None:intergenic
TTGCTCAAGATATTTCATGG+AGG 0.720826 8:-78456594 MsG0880046805.01.T01:three_prime_UTR
CATGATCATGATCATCTGAG+TGG 0.721603 8:+78457191 None:intergenic
AAATGATGTTGAAGATCACG+TGG 0.746908 8:-78456938 MsG0880046805.01.T01:CDS
TATCATGAGAACCATCAATG+GGG 0.763816 8:+78456914 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GTACATTAATATTATTTATA+TGG - Chr8:78456930-78456949 MsG0880046805.01.T01:CDS 10.0%
!! AATTAGTAGTACTAATTATT+TGG + Chr8:78457271-78457290 None:intergenic 15.0%
!! GTAACTAATAATATAATTAC+TGG + Chr8:78456878-78456897 None:intergenic 15.0%
!! AAGTACTAACATTGAAATAT+TGG + Chr8:78456904-78456923 None:intergenic 20.0%
!! AATAATATAATTACTGGTGA+TGG + Chr8:78456872-78456891 None:intergenic 20.0%
! GTATATATAGTGTATATGCA+CGG - Chr8:78457298-78457317 MsG0880046805.01.T01:exon 25.0%
! TCTCTTTGAATCATGATAAT+AGG - Chr8:78456706-78456725 MsG0880046805.01.T01:intron 25.0%
! TTAGTACTACTAATTAGTTG+AGG - Chr8:78457276-78457295 MsG0880046805.01.T01:CDS 25.0%
ATGGTTCTCATGATAATCAT+AGG - Chr8:78457098-78457117 MsG0880046805.01.T01:intron 30.0%
ATTATCATGAGAACCATCAA+TGG + Chr8:78457095-78457114 None:intergenic 30.0%
TAATCATAGGAATTACGTGA+AGG - Chr8:78457111-78457130 MsG0880046805.01.T01:intron 30.0%
TATCATGATTCAAAGAGAAG+TGG + Chr8:78456704-78456723 None:intergenic 30.0%
TCATGTAGTTGGAAATTAAC+AGG - Chr8:78457328-78457347 MsG0880046805.01.T01:five_prime_UTR 30.0%
TTATCATGAGAACCATCAAT+GGG + Chr8:78457094-78457113 None:intergenic 30.0%
TTCTTGCTCAAGATATTTCA+TGG - Chr8:78457407-78457426 MsG0880046805.01.T01:five_prime_UTR 30.0%
!! AATATAATTACTGGTGATGG+TGG + Chr8:78456869-78456888 None:intergenic 30.0%
AAATGATGTTGAAGATCACG+TGG - Chr8:78457066-78457085 MsG0880046805.01.T01:intron 35.0%
ACGGCATTAATTCATGTAGT+TGG - Chr8:78457317-78457336 MsG0880046805.01.T01:five_prime_UTR 35.0%
ATCAAGACAAAGAAGGTGAT+TGG - Chr8:78457383-78457402 MsG0880046805.01.T01:five_prime_UTR 35.0%
CAAACATGTCCATCAAAGTT+TGG + Chr8:78457207-78457226 None:intergenic 35.0%
CACAATATGGAGCTTCATTT+GGG - Chr8:78456621-78456640 MsG0880046805.01.T01:three_prime_UTR 35.0%
CGGACGAGAATTATTATAAG+TGG + Chr8:78456658-78456677 None:intergenic 35.0%
CTCTCTACACAATCACAATA+TGG - Chr8:78456608-78456627 MsG0880046805.01.T01:three_prime_UTR 35.0%
CTGGTTTATCAAGACAAAGA+AGG - Chr8:78457376-78457395 MsG0880046805.01.T01:five_prime_UTR 35.0%
GAATTACGTGAAGGTGAAAA+TGG - Chr8:78457120-78457139 MsG0880046805.01.T01:intron 35.0%
TACTCTCAACCAAACTTTGA+TGG - Chr8:78457195-78457214 MsG0880046805.01.T01:CDS 35.0%
TATCATGAGAACCATCAATG+GGG + Chr8:78457093-78457112 None:intergenic 35.0%
TCACAATATGGAGCTTCATT+TGG - Chr8:78456620-78456639 MsG0880046805.01.T01:three_prime_UTR 35.0%
TGATCAACAACAGTATGAAC+TGG - Chr8:78457357-78457376 MsG0880046805.01.T01:five_prime_UTR 35.0%
TTAGTAGCAGCAATATCGTA+CGG + Chr8:78456678-78456697 None:intergenic 35.0%
TTCTTCCTCCTACAATTAAC+CGG + Chr8:78457031-78457050 None:intergenic 35.0%
TTGCTCAAGATATTTCATGG+AGG - Chr8:78457410-78457429 MsG0880046805.01.T01:five_prime_UTR 35.0%
! GGTTGAGAGTATGAAATGAA+TGG + Chr8:78457186-78457205 None:intergenic 35.0%
!!! CGTAGTTTTTTCTCAACTCT+TGG - Chr8:78456732-78456751 MsG0880046805.01.T01:intron 35.0%
ATGAAGAAGCTCTTGTTGGA+TGG - Chr8:78457002-78457021 MsG0880046805.01.T01:CDS 40.0%
CATGATCATGATCATCTGAG+TGG + Chr8:78456816-78456835 None:intergenic 40.0%
GATGATGAAGAAGCTCTTGT+TGG - Chr8:78456998-78457017 MsG0880046805.01.T01:CDS 40.0%
TACGTGAAGGTGAAAATGGA+AGG - Chr8:78457124-78457143 MsG0880046805.01.T01:intron 40.0%
! AAGGTGAAAATGGAAGGTGT+AGG - Chr8:78457130-78457149 MsG0880046805.01.T01:intron 40.0%
AATGGGAGGAGAGAAAGAGT+GGG + Chr8:78456784-78456803 None:intergenic 45.0%
AGGTGTAGGAATAGCTCGAA+AGG - Chr8:78457144-78457163 MsG0880046805.01.T01:intron 45.0%
TAATGGGAGGAGAGAAAGAG+TGG + Chr8:78456785-78456804 None:intergenic 45.0%
TTCCTCCTACAATTAACCGG+AGG + Chr8:78457028-78457047 None:intergenic 45.0%
!! ATTACTGGTGATGGTGGTAG+TGG + Chr8:78456863-78456882 None:intergenic 45.0%
!! ATTATTTATTTGATTAATTT+AGG - Chr8:78456966-78456985 MsG0880046805.01.T01:CDS 5.0%
AGAAAGAGTGGGAAGGTTGG+TGG + Chr8:78456773-78456792 None:intergenic 50.0%
CTGAGTGGCCAGGAGTTAAT+GGG + Chr8:78456801-78456820 None:intergenic 50.0%
GAGAGAAAGAGTGGGAAGGT+TGG + Chr8:78456776-78456795 None:intergenic 50.0%
GATGGCCTCCGGTTAATTGT+AGG - Chr8:78457020-78457039 MsG0880046805.01.T01:intron 50.0%
TCATGATCATCTGAGTGGCC+AGG + Chr8:78456811-78456830 None:intergenic 50.0%
TCTCTCCTCCCATTAACTCC+TGG - Chr8:78456790-78456809 MsG0880046805.01.T01:intron 50.0%
TCTGAGTGGCCAGGAGTTAA+TGG + Chr8:78456802-78456821 None:intergenic 50.0%
AGCTCTTGTTGGATGGCCTC+CGG - Chr8:78457009-78457028 MsG0880046805.01.T01:CDS 55.0%
AGTGGCCAGGAGTTAATGGG+AGG + Chr8:78456798-78456817 None:intergenic 55.0%
GATCACGTGGTCCCCATTGA+TGG - Chr8:78457079-78457098 MsG0880046805.01.T01:intron 55.0%
GGAGGAGAGAAAGAGTGGGA+AGG + Chr8:78456780-78456799 None:intergenic 55.0%
GGCCTCCGGTTAATTGTAGG+AGG - Chr8:78457023-78457042 MsG0880046805.01.T01:intron 55.0%
Chromosome Type Strat End Strand Name
Chr8 gene 78456591 78457435 78456591 ID=MsG0880046805.01;Name=MsG0880046805.01
Chr8 mRNA 78456591 78457435 78456591 ID=MsG0880046805.01.T01;Parent=MsG0880046805.01;Name=MsG0880046805.01.T01;_AED=0.20;_eAED=0.20;_QI=127|1|0.66|1|0.5|0.66|3|188|100
Chr8 exon 78456591 78456676 78456591 ID=MsG0880046805.01.T01:exon:18783;Parent=MsG0880046805.01.T01
Chr8 exon 78456794 78457038 78456794 ID=MsG0880046805.01.T01:exon:18782;Parent=MsG0880046805.01.T01
Chr8 exon 78457149 78457435 78457149 ID=MsG0880046805.01.T01:exon:18781;Parent=MsG0880046805.01.T01
Chr8 five_prime_UTR 78457309 78457435 78457309 ID=MsG0880046805.01.T01:five_prime_utr;Parent=MsG0880046805.01.T01
Chr8 CDS 78457149 78457308 78457149 ID=MsG0880046805.01.T01:cds;Parent=MsG0880046805.01.T01
Chr8 CDS 78456896 78457038 78456896 ID=MsG0880046805.01.T01:cds;Parent=MsG0880046805.01.T01
Chr8 three_prime_UTR 78456794 78456895 78456794 ID=MsG0880046805.01.T01:three_prime_utr;Parent=MsG0880046805.01.T01
Chr8 three_prime_UTR 78456591 78456676 78456591 ID=MsG0880046805.01.T01:three_prime_utr;Parent=MsG0880046805.01.T01
Gene Sequence

>MsG0880046805.01.T01

ATGATAATAGGAAACGTAGTTTTTTCTCAACTCTTGGAGCATGCAGCAGAAACCACCAACCTTCCCACTCTTTCTCTCCTCCCATTAACTCCTGGCCACTCAGATGATCATGATCATGATGATCAACACTGCTCTCACAGCTCCACTACCACCATCACCAGAGCAATGAAGATGATGAAGAAGCTCTTGTTGGATGGCCTCCGGTTAATTGTAGGAGGAAGAAAATTCGTTCTAATGAAAATGATGTTGAAGATCACGTGGTCCCCATTGATGGTTCTCATGATAATCATAGGAATTACGTGA

Protein sequence

>MsG0880046805.01.T01

MIIGNVVFSQLLEHAAETTNLPTLSLLPLTPGHSDDHDHDDQHCSHSSTTTITRAMKMMKKLLLDGLRLIVGGRKFVLMKMMLKITWSPLMVLMIIIGIT*