AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0558880


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 6 sgRNAs with CRISPR-Local

Find 70 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGCAAGAGTCGTCAGCTTC+AGG 0.341073 4_1:+57914955 Msa0558870:CDS
GTTCTGAGATGCATTCAAAT+AGG 0.382160 4_1:+57914805 Msa0558870:CDS
AAAACAACATCTAACATTGA+TGG 0.412438 4_1:-57914862 Msa0558880:CDS
CTGACGACTCTTGCAACTCT+TGG 0.431973 4_1:-57914948 Msa0558880:CDS
GAGTCGTCAGCTTCAGGAGG+AGG 0.457899 4_1:+57914961 Msa0558870:CDS
CAAGAGTCGTCAGCTTCAGG+AGG 0.635040 4_1:+57914958 Msa0558870:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATCAAATATGTAATGTTT+CGG + chr4_1:57915352-57915371 None:intergenic 15.0%
!! AAACATTACATATTTGATTA+AGG - chr4_1:57915352-57915371 Msa0558880:intron 15.0%
!! TAATCAAATATGTAATGTTT+CGG + chr4_1:57915352-57915371 None:intergenic 15.0%
!! AAACATTACATATTTGATTA+AGG - chr4_1:57915352-57915371 Msa0558880:intron 15.0%
!! TTATTGAGTTACTTACATAA+TGG + chr4_1:57914947-57914966 Msa0558870:CDS 20.0%
!! CAATTCTGATAAAAAAAGTA+TGG - chr4_1:57915325-57915344 Msa0558880:intron 20.0%
!! TTATTGAGTTACTTACATAA+TGG + chr4_1:57914947-57914966 Msa0558870:CDS 20.0%
!! CAATTCTGATAAAAAAAGTA+TGG - chr4_1:57915325-57915344 Msa0558880:intron 20.0%
! GATCATGTAAATGTATTACA+AGG - chr4_1:57914915-57914934 Msa0558880:CDS 25.0%
!! TTAATCTTTGAGAATTCAAG+TGG - chr4_1:57915048-57915067 Msa0558880:intron 25.0%
!! ATTTTATACTTATGCACAGT+TGG - chr4_1:57915164-57915183 Msa0558880:intron 25.0%
! CATAAAATCTTGTATATACC+AGG - chr4_1:57915216-57915235 Msa0558880:intron 25.0%
!! AAACCAGCATTTTTATTCAA+CGG + chr4_1:57915469-57915488 None:intergenic 25.0%
!!! AATAAAAATGCTGGTTTTAG+AGG - chr4_1:57915472-57915491 Msa0558880:intron 25.0%
! AAAACAACATCTAACATTGA+TGG - chr4_1:57915526-57915545 Msa0558880:intron 25.0%
! GATCATGTAAATGTATTACA+AGG - chr4_1:57914915-57914934 Msa0558880:CDS 25.0%
!! TTAATCTTTGAGAATTCAAG+TGG - chr4_1:57915048-57915067 Msa0558880:intron 25.0%
!! ATTTTATACTTATGCACAGT+TGG - chr4_1:57915164-57915183 Msa0558880:intron 25.0%
! CATAAAATCTTGTATATACC+AGG - chr4_1:57915216-57915235 Msa0558880:intron 25.0%
!! AAACCAGCATTTTTATTCAA+CGG + chr4_1:57915469-57915488 None:intergenic 25.0%
!!! AATAAAAATGCTGGTTTTAG+AGG - chr4_1:57915472-57915491 Msa0558880:intron 25.0%
! AAAACAACATCTAACATTGA+TGG - chr4_1:57915526-57915545 Msa0558880:intron 25.0%
CTAGCATCATACAATAAACA+CGG + chr4_1:57914860-57914879 Msa0558870:CDS 30.0%
! ATGATGCTAGTGTATTTTAC+AGG - chr4_1:57914870-57914889 Msa0558880:CDS 30.0%
! TGATGCTAGTGTATTTTACA+GGG - chr4_1:57914871-57914890 Msa0558880:CDS 30.0%
! TGGAAATTTCTAAGAGCTTA+AGG + chr4_1:57915111-57915130 Msa0558870:three_prime_UTR 30.0%
AAGCTCTTAGAAATTTCCAA+TGG - chr4_1:57915112-57915131 Msa0558880:intron 30.0%
AAAATCTTGTATATACCAGG+AGG - chr4_1:57915219-57915238 Msa0558880:intron 30.0%
! GGGTATTGTAAAATGAATAC+TGG + chr4_1:57915251-57915270 None:intergenic 30.0%
CTAGCATCATACAATAAACA+CGG + chr4_1:57914860-57914879 Msa0558870:CDS 30.0%
! ATGATGCTAGTGTATTTTAC+AGG - chr4_1:57914870-57914889 Msa0558880:CDS 30.0%
! TGATGCTAGTGTATTTTACA+GGG - chr4_1:57914871-57914890 Msa0558880:CDS 30.0%
! TGGAAATTTCTAAGAGCTTA+AGG + chr4_1:57915111-57915130 Msa0558870:three_prime_UTR 30.0%
AAGCTCTTAGAAATTTCCAA+TGG - chr4_1:57915112-57915131 Msa0558880:intron 30.0%
AAAATCTTGTATATACCAGG+AGG - chr4_1:57915219-57915238 Msa0558880:intron 30.0%
! GGGTATTGTAAAATGAATAC+TGG + chr4_1:57915251-57915270 None:intergenic 30.0%
! TTTTACAGGGAACATCACTA+AGG - chr4_1:57914884-57914903 Msa0558880:CDS 35.0%
! AAGTATGTGTAGTCACTTTG+GGG + chr4_1:57915271-57915290 None:intergenic 35.0%
! CAAGTATGTGTAGTCACTTT+GGG + chr4_1:57915272-57915291 None:intergenic 35.0%
ACAAGTATGTGTAGTCACTT+TGG + chr4_1:57915273-57915292 None:intergenic 35.0%
! GAGTTTGTAGACATTTCTAG+AGG + chr4_1:57915306-57915325 None:intergenic 35.0%
TTGCCGTTGAATAAAAATGC+TGG - chr4_1:57915463-57915482 Msa0558880:intron 35.0%
!!! AAAAATGCTGGTTTTAGAGG+TGG - chr4_1:57915475-57915494 Msa0558880:intron 35.0%
!!! AAAATGCTGGTTTTAGAGGT+GGG - chr4_1:57915476-57915495 Msa0558880:intron 35.0%
! TTTTACAGGGAACATCACTA+AGG - chr4_1:57914884-57914903 Msa0558880:CDS 35.0%
! AAGTATGTGTAGTCACTTTG+GGG + chr4_1:57915271-57915290 None:intergenic 35.0%
! CAAGTATGTGTAGTCACTTT+GGG + chr4_1:57915272-57915291 None:intergenic 35.0%
ACAAGTATGTGTAGTCACTT+TGG + chr4_1:57915273-57915292 None:intergenic 35.0%
! GAGTTTGTAGACATTTCTAG+AGG + chr4_1:57915306-57915325 None:intergenic 35.0%
TTGCCGTTGAATAAAAATGC+TGG - chr4_1:57915463-57915482 Msa0558880:intron 35.0%
!!! AAAAATGCTGGTTTTAGAGG+TGG - chr4_1:57915475-57915494 Msa0558880:intron 35.0%
!!! AAAATGCTGGTTTTAGAGGT+GGG - chr4_1:57915476-57915495 Msa0558880:intron 35.0%
! ACTCGTGAGTTTTCTACCAT+TGG + chr4_1:57915131-57915150 Msa0558870:three_prime_UTR 40.0%
! ACTCGTGAGTTTTCTACCAT+TGG + chr4_1:57915131-57915150 Msa0558870:three_prime_UTR 40.0%
CTATGGTTGAAGCTCGCTAA+AGG - chr4_1:57914834-57914853 Msa0558880:CDS 45.0%
!!! GAATACTGGTTTTGACCTCC+TGG + chr4_1:57915237-57915256 None:intergenic 45.0%
!!! TAGCTTTTAGCGAGCACTGA+TGG - chr4_1:57915375-57915394 Msa0558880:intron 45.0%
CTATGGTTGAAGCTCGCTAA+AGG - chr4_1:57914834-57914853 Msa0558880:CDS 45.0%
!!! GAATACTGGTTTTGACCTCC+TGG + chr4_1:57915237-57915256 None:intergenic 45.0%
!!! TAGCTTTTAGCGAGCACTGA+TGG - chr4_1:57915375-57915394 Msa0558880:intron 45.0%
TTGCAAGAGTCGTCAGCTTC+AGG + chr4_1:57915436-57915455 None:intergenic 50.0%
CTGACGACTCTTGCAACTCT+TGG - chr4_1:57915440-57915459 Msa0558880:intron 50.0%
!!! GGTTTTAGAGGTGGGCAAAG+AGG - chr4_1:57915484-57915503 Msa0558880:intron 50.0%
TTGCAAGAGTCGTCAGCTTC+AGG + chr4_1:57915436-57915455 None:intergenic 50.0%
CTGACGACTCTTGCAACTCT+TGG - chr4_1:57915440-57915459 Msa0558880:intron 50.0%
!!! GGTTTTAGAGGTGGGCAAAG+AGG - chr4_1:57915484-57915503 Msa0558880:intron 50.0%
CAAGAGTCGTCAGCTTCAGG+AGG + chr4_1:57915433-57915452 None:intergenic 55.0%
CAAGAGTCGTCAGCTTCAGG+AGG + chr4_1:57915433-57915452 None:intergenic 55.0%
GAGTCGTCAGCTTCAGGAGG+AGG + chr4_1:57915430-57915449 None:intergenic 60.0%
GAGTCGTCAGCTTCAGGAGG+AGG + chr4_1:57915430-57915449 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 57914819 57915591 57914819 ID=Msa0558880;Name=Msa0558880
chr4_1 mRNA 57914819 57915591 57914819 ID=Msa0558880-mRNA-1;Parent=Msa0558880;Name=Msa0558880-mRNA-1;_AED=0.97;_eAED=1.00;_QI=0|0|0|0.66|1|1|3|0|72
chr4_1 exon 57915587 57915591 57915587 ID=Msa0558880-mRNA-1:exon:17638;Parent=Msa0558880-mRNA-1
chr4_1 exon 57915344 57915350 57915344 ID=Msa0558880-mRNA-1:exon:17637;Parent=Msa0558880-mRNA-1
chr4_1 exon 57914819 57915025 57914819 ID=Msa0558880-mRNA-1:exon:17636;Parent=Msa0558880-mRNA-1
chr4_1 CDS 57915587 57915591 57915587 ID=Msa0558880-mRNA-1:cds;Parent=Msa0558880-mRNA-1
chr4_1 CDS 57915344 57915350 57915344 ID=Msa0558880-mRNA-1:cds;Parent=Msa0558880-mRNA-1
chr4_1 CDS 57914819 57915025 57914819 ID=Msa0558880-mRNA-1:cds;Parent=Msa0558880-mRNA-1
Gene Sequence

>Msa0558880

ATGAGAATTCAACGAGCACTGATGGTAGTTTCTGTTAATTCATTTATTGAAGATCCTCCTCCTGAAGCTGACGACTCTTGCAACTCTTGGTTGCCGTTGAATAAAAATGCTGGTTTTAGAGGTGGGCAAAGAGGTGTTTCATTGCATAGCATGAAAACAACATCTAACATTGATGGTCTATTAGTTGCATTTTCTTGCACACACAAGAGTCCTATTTGA

Protein sequence

>Msa0558880

MRIQRALMVVSVNSFIEDPPPEADDSCNSWLPLNKNAGFRGGQRGVSLHSMKTTSNIDGLLVAFSCTHKSPI*