AlfalfaGEDB | Msa0705060

M. sativa cultivar zhongmu-4 / Msa0705060

KEGG: K07897|K07976
GO: -
PFAM: PF00071.25

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0705060	XP_003630119.1	98.086	209	4	0	1	209	1	209	5.30e-153	432

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0705060	sp\|Q43463\|RAB7_SOYBN	79.024	205	42	1	6	209	2	206	7.56e-122	346

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0705060	G7LBB5	98.086	209	4	0	1	209	1	209	2.53e-153	432

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0003470	Msa0705060	0.833084	6.534093e-56	-8.615850e-47
Msa0004350	Msa0705060	0.814463	1.520972e-51	-8.615850e-47
Msa0008850	Msa0705060	0.818767	1.650622e-52	-8.615850e-47
Msa0017120	Msa0705060	0.818578	1.821730e-52	-8.615850e-47
Msa0024680	Msa0705060	0.809665	1.691597e-50	-8.615850e-47
Msa0027540	Msa0705060	0.827218	1.766329e-54	-8.615850e-47
Msa0028060	Msa0705060	0.802978	4.340951e-49	-8.615850e-47
Msa0030000	Msa0705060	0.807451	5.022677e-50	-8.615850e-47
Msa0047470	Msa0705060	0.804145	2.486087e-49	-8.615850e-47
Msa0050430	Msa0705060	0.815532	8.807976e-52	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0705060	MtrunA17_Chr8g0383261	98.086	209	4	0	1	209	1	209	4.87e-157	432
Msa0705060	MtrunA17_Chr6g0466301	76.847	203	46	1	8	209	4	206	1.13e-116	330
Msa0705060	MtrunA17_Chr6g0465491	76.847	203	46	1	8	209	4	206	1.13e-116	330
Msa0705060	MtrunA17_Chr1g0201121	74.757	206	50	1	6	209	2	207	1.59e-115	327
Msa0705060	MtrunA17_Chr2g0331691	65.700	207	71	0	3	209	1	207	1.78e-102	294
Msa0705060	MtrunA17_Chr4g0034871	67.488	203	65	1	8	209	4	206	1.02e-100	290
Msa0705060	MtrunA17_Chr4g0004661	66.337	202	68	0	8	209	60	261	1.39e-100	291
Msa0705060	MtrunA17_Chr6g0466161	65.193	181	63	0	29	209	7	187	4.11e-83	245
Msa0705060	MtrunA17_Chr6g0465471	64.641	181	64	0	29	209	7	187	1.82e-82	244
Msa0705060	MtrunA17_Chr6g0466431	62.617	107	31	1	8	114	4	101	5.13e-44	142

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0705060	AT1G22740.1	75.490	204	48	1	6	209	2	203	5.52e-113	321
Msa0705060	AT4G09720.1	71.429	203	57	1	8	209	4	206	4.25e-110	314
Msa0705060	AT4G09720.4	70.192	208	56	3	8	209	4	211	1.32e-106	305
Msa0705060	AT4G09720.3	67.757	214	57	2	8	209	4	217	2.21e-106	305
Msa0705060	AT2G21880.1	70.000	210	57	2	6	209	3	212	6.64e-103	296
Msa0705060	AT1G52280.1	67.317	205	66	1	6	209	2	206	9.97e-103	295
Msa0705060	AT3G16100.1	66.341	205	68	1	6	209	2	206	1.80e-101	292
Msa0705060	AT3G18820.1	67.488	203	65	1	8	209	4	206	3.82e-100	289
Msa0705060	AT1G49300.2	66.176	204	68	1	7	209	3	206	1.18e-99	288
Msa0705060	AT1G49300.1	66.176	204	68	1	7	209	3	206	1.18e-99	288

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Find 53 sgRNAs with CRISPR-Local

Find 280 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATGCAGCTCCAAGACTA+TGG	0.389043	4_4:+77581435	None:intergenic
AACAAAATCAGCCCCAATTG+TGG	0.543201	4_4:+77581639	None:intergenic
AACAAGGTTGATATAGATGG+TGG	0.570432	4_4:-77580804	Msa0705060:CDS
AACAGTATAAAGCCACAATT+GGG	0.408620	4_4:-77581651	Msa0705060:CDS
AAGGGGTTTCAGATATTCCA+CGG	0.600915	4_4:+77580394	None:intergenic
AATACAAAGGGAAATGCCTC+AGG	0.512303	4_4:+77580834	None:intergenic
AATTCTATTATCCGTAAGTA+TGG	0.278709	4_4:-77580227	Msa0705060:three_prime_UTR
AATTGATCTCAGGCAGATAC+AGG	0.484106	4_4:-77580861	Msa0705060:intron
ACAGTATAAAGCCACAATTG+GGG	0.725981	4_4:-77581650	Msa0705060:CDS
AGAAGGATTATCCATACTTA+CGG	0.530605	4_4:+77580216	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TGAACTAGAAATATCCATGT+TGG	+	chr4_4:77580128-77580147	None:intergenic	30.0%
GACATGAAAACATATGAAGA+AGG	+	chr4_4:77580215-77580234	None:intergenic	30.0%
TAGATTGCATGTAAATTTGG+TGG	+	chr4_4:77580679-77580698	None:intergenic	30.0%
AACAGTATAAAGCCACAATT+GGG	-	chr4_4:77580838-77580857	Msa0705060:CDS	30.0%
AAGATTGTAAGCCAAACAAA+GGG	+	chr4_4:77581246-77581265	None:intergenic	30.0%
TAAGATTGTAAGCCAAACAA+AGG	+	chr4_4:77581247-77581266	None:intergenic	30.0%
AATCATTGGATCAAATGGAA+CGG	-	chr4_4:77581322-77581341	Msa0705060:intron	30.0%
TTTATCTTCCGATAGAATCT+TGG	-	chr4_4:77581498-77581517	Msa0705060:intron	30.0%
TTTGATGTGAAATTGATCTC+AGG	-	chr4_4:77581618-77581637	Msa0705060:CDS	30.0%
CATTTCCCTTTGTATTACTT+GGG	-	chr4_4:77581662-77581681	Msa0705060:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_4	gene	77580089	77582422	77580089	ID=Msa0705060;Name=Msa0705060
chr4_4	mRNA	77580089	77582422	77580089	ID=Msa0705060-mRNA-1;Parent=Msa0705060;Name=Msa0705060-mRNA-1;_AED=0.14;_eAED=0.14;_QI=41\|1\|1\|1\|1\|1\|7\|270\|209
chr4_4	exon	77580089	77580422	77580089	ID=Msa0705060-mRNA-1:exon:24654;Parent=Msa0705060-mRNA-1
chr4_4	exon	77580537	77580682	77580537	ID=Msa0705060-mRNA-1:exon:24653;Parent=Msa0705060-mRNA-1
chr4_4	exon	77580791	77580871	77580791	ID=Msa0705060-mRNA-1:exon:24652;Parent=Msa0705060-mRNA-1
chr4_4	exon	77581340	77581486	77581340	ID=Msa0705060-mRNA-1:exon:24651;Parent=Msa0705060-mRNA-1
chr4_4	exon	77581597	77581696	77581597	ID=Msa0705060-mRNA-1:exon:24650;Parent=Msa0705060-mRNA-1
chr4_4	exon	77582172	77582198	77582172	ID=Msa0705060-mRNA-1:exon:24649;Parent=Msa0705060-mRNA-1
chr4_4	exon	77582317	77582422	77582317	ID=Msa0705060-mRNA-1:exon:24648;Parent=Msa0705060-mRNA-1
chr4_4	five_prime_UTR	77582382	77582422	77582382	ID=Msa0705060-mRNA-1:five_prime_utr;Parent=Msa0705060-mRNA-1
chr4_4	CDS	77582317	77582381	77582317	ID=Msa0705060-mRNA-1:cds;Parent=Msa0705060-mRNA-1
chr4_4	CDS	77582172	77582198	77582172	ID=Msa0705060-mRNA-1:cds;Parent=Msa0705060-mRNA-1
chr4_4	CDS	77581597	77581696	77581597	ID=Msa0705060-mRNA-1:cds;Parent=Msa0705060-mRNA-1
chr4_4	CDS	77581340	77581486	77581340	ID=Msa0705060-mRNA-1:cds;Parent=Msa0705060-mRNA-1
chr4_4	CDS	77580791	77580871	77580791	ID=Msa0705060-mRNA-1:cds;Parent=Msa0705060-mRNA-1
chr4_4	CDS	77580537	77580682	77580537	ID=Msa0705060-mRNA-1:cds;Parent=Msa0705060-mRNA-1
chr4_4	CDS	77580359	77580422	77580359	ID=Msa0705060-mRNA-1:cds;Parent=Msa0705060-mRNA-1
chr4_4	three_prime_UTR	77580089	77580358	77580089	ID=Msa0705060-mRNA-1:three_prime_utr;Parent=Msa0705060-mRNA-1

Gene Sequence

>Msa0705060

ATGGATATTTCTAGTTCACACAGAAAGAGAACTTTGCTTAAGGTCATTGTCCTTGGAGATAGTGGGGTTGGGAAGACATCTTTGATGAACCAATATGTGTATAAAAAATTTAGCCAACAGTATAAAGCCACAATTGGGGCTGATTTTGTTACAAAGGAGCTGCTAGTTGATGATAAACTCGTAACCTTGCAGATTTGGGATACTGCAGGACAGGAAAGGTTCCATAGTCTTGGAGCTGCATTTTACAGAGGGGCAGATTGTTGTGTTTTGGTATATGATGTAAATATACACAAAACATTTGATACATTAAACAATTGGCATGATGACTTTCTTAAGCAGGCAGATACAGGCAATCCTGAGGCATTTCCCTTTGTATTACTTGGGAACAAGGTTGATATAGATGGTGGAAACAGTAGAAGGGTTACAGAGAAGAAAGCTAGGGAATGGTGTGCTTCTAGGGGTAACATACCATATTTTGAAACCTCTGCAAAAGAGGGCTACAATGTTGATGATGCATTTTTATGTGTTGCTAAAGTTGCATCAGAAAATGAACACGATTTAGACATTTATTTCCGTGGAATATCTGAAACCCCTTCAGAAGCAGAACAACGAAGTAATTGTGCATGCTGA

Protein sequence

>Msa0705060

MDISSSHRKRTLLKVIVLGDSGVGKTSLMNQYVYKKFSQQYKATIGADFVTKELLVDDKLVTLQIWDTAGQERFHSLGAAFYRGADCCVLVYDVNIHKTFDTLNNWHDDFLKQADTGNPEAFPFVLLGNKVDIDGGNSRRVTEKKAREWCASRGNIPYFETSAKEGYNVDDAFLCVAKVASENEHDLDIYFRGISETPSEAEQRSNCAC*