Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0557630 | XP_024638882.1 | 97.133 | 279 | 8 | 0 | 1 | 279 | 1 | 279 | 0.0 | 561 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0557630 | A0A396I9U6 | 97.133 | 279 | 8 | 0 | 1 | 279 | 1 | 279 | 0.0 | 561 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0003470 | Msa0557630 | 0.826280 | 2.957931e-54 | -8.615850e-47 |
| Msa0004350 | Msa0557630 | 0.843058 | 1.769285e-58 | -8.615850e-47 |
| Msa0006700 | Msa0557630 | 0.816352 | 5.781737e-52 | -8.615850e-47 |
| Msa0006820 | Msa0557630 | 0.825671 | 4.128838e-54 | -8.615850e-47 |
| Msa0008330 | Msa0557630 | 0.818385 | 2.015260e-52 | -8.615850e-47 |
| Msa0008850 | Msa0557630 | 0.837776 | 4.257849e-57 | -8.615850e-47 |
| Msa0011720 | Msa0557630 | 0.843407 | 1.428222e-58 | -8.615850e-47 |
| Msa0012440 | Msa0557630 | 0.840645 | 7.676978e-58 | -8.615850e-47 |
| Msa0017120 | Msa0557630 | 0.846936 | 1.587266e-59 | -8.615850e-47 |
| Msa0017940 | Msa0557630 | 0.803430 | 3.499710e-49 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0557630 | MtrunA17_Chr4g0046891 | 97.133 | 279 | 8 | 0 | 1 | 279 | 1 | 279 | 0.0 | 561 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0557630 | AT4G26415.1 | 45.299 | 234 | 61 | 10 | 3 | 192 | 7 | 217 | 3.23e-44 | 152 |
Find 59 sgRNAs with CRISPR-Local
Find 148 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAACTAGCAATGGACATGC+AGG | 0.491726 | 4_1:-56079689 | Msa0557630:CDS |
| AAACTAGCAATGGACATGCA+GGG | 0.676573 | 4_1:-56079688 | Msa0557630:CDS |
| AAATGGAGGAAGAGATGGCA+AGG | 0.533678 | 4_1:-56080125 | Msa0557630:CDS |
| AACAGTTGCTGGGGAGAACG+TGG | 0.612915 | 4_1:-56079872 | Msa0557630:CDS |
| AACCAACAAAATAGTACTAT+TGG | 0.366556 | 4_1:-56080276 | Msa0557630:CDS |
| AACGTGGTTTGGTGTAAACC+TGG | 0.608504 | 4_1:-56079856 | Msa0557630:CDS |
| AATATAGTGGAATAAGGGGA+AGG | 0.620558 | 4_1:-56080086 | Msa0557630:CDS |
| AATGAATATAGTGGAATAAG+GGG | 0.579962 | 4_1:-56080090 | Msa0557630:CDS |
| ACATTGTTAGAAGAAGAAAT+AGG | 0.529346 | 4_1:-56079654 | Msa0557630:CDS |
| ACCGGTTTCTCTAGAAGCTA+TGG | 0.522607 | 4_1:-56079593 | Msa0557630:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| CAAAATAGTACTATTGGCAA+TGG | - | chr4_1:56079651-56079670 | Msa0557630:CDS | 30.0% | |
| AGTTGTCACATTGCAAAAAA+GGG | + | chr4_1:56079725-56079744 | None:intergenic | 30.0% | |
| AAAGATCTGACAAAAAAGAG+AGG | - | chr4_1:56080141-56080160 | Msa0557630:CDS | 30.0% | |
| GTAGGAGACAAAATATAAGA+AGG | - | chr4_1:56080180-56080199 | Msa0557630:CDS | 30.0% | |
| CAAAATAGTACTATTGGCAA+TGG | - | chr4_1:56079651-56079670 | Msa0557630:CDS | 30.0% | |
| AGTTGTCACATTGCAAAAAA+GGG | + | chr4_1:56079725-56079744 | None:intergenic | 30.0% | |
| AAAGATCTGACAAAAAAGAG+AGG | - | chr4_1:56080141-56080160 | Msa0557630:CDS | 30.0% | |
| GTAGGAGACAAAATATAAGA+AGG | - | chr4_1:56080180-56080199 | Msa0557630:CDS | 30.0% | |
| TTTCTCCTCAAGAACTCTTT+GGG | - | chr4_1:56079561-56079580 | Msa0557630:CDS | 35.0% | |
| TCTTATGCCTTTCTTCATCT+TGG | + | chr4_1:56079589-56079608 | None:intergenic | 35.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_1 | gene | 56079552 | 56080391 | 56079552 | ID=Msa0557630;Name=Msa0557630 |
| chr4_1 | mRNA | 56079552 | 56080391 | 56079552 | ID=Msa0557630-mRNA-1;Parent=Msa0557630;Name=Msa0557630-mRNA-1;_AED=0.37;_eAED=0.37;_QI=0|-1|0|1|-1|1|1|0|279 |
| chr4_1 | exon | 56079552 | 56080391 | 56079552 | ID=Msa0557630-mRNA-1:exon:16776;Parent=Msa0557630-mRNA-1 |
| chr4_1 | CDS | 56079552 | 56080391 | 56079552 | ID=Msa0557630-mRNA-1:cds;Parent=Msa0557630-mRNA-1 |
| Gene Sequence |
>Msa0557630 ATGCCATTCTTTCTCCTCAAGAACTCTTTGGGAGCCAAGATGAAGAAAGGCATAAGAACTTTCTGCAACACTGATGTGTCAACCTCAACTCTAAACCAACAAAATAGTACTATTGGCAATGGCATTGGCAACCATAGCCAAGTTGACATCACAAGCAGCAAGGTAAGCTCCCCTTTTTTGCAATGTGACAACTCAAATTCAAAGCAAAGCACACCAACATTAGAAGATCTTATACTACAATTAGAAATGGAGGAAGAGATGGCAAGGAAAGAAAAACTCAATGAATATAGTGGAATAAGGGGAAGGATGTCATGTGTTAACAACTCTGACATATTAAGATCTGCAAGGAATGCTTTGAATCAATATCCAAGGTTTTCACTTGATGGAAGAGATTCCATGTATAGGTCATCTTTTGGGACTATAGAAGGAAGAAGATCAGTTTGTAGTGAGATAAGTTTAGAACCAAAGCTTGATGAAAAGGTTATGTGTTTTCCACCAACAGTTGCTGGGGAGAACGTGGTTTGGTGTAAACCTGGTGTTGTGGCAAAGTTAATGGGTTTGGAAGCTATTCCAGTGTCAGTAGGTAGAAAAAGATCTGACAAAAAAGAGAGGATGAGTAGTGTTGATTGTAGGAGACAAAATATAAGAAGGAGGCCATTTGAGAGGCATGATTTGGAGAGAAAACTAGCAATGGACATGCAGGGTTATCGTGATCACATTGTTAGAAGAAGAAATAGGTCTGGTTTTTGCTCTAAAAATGGATATTGTATTATGAAACCGGTTTCTCTAGAAGCTATGGCAGGAGGTCCTAGTAGTTGGCAACCAAGGAGATATGTTTAG |
| Protein sequence |
>Msa0557630 MPFFLLKNSLGAKMKKGIRTFCNTDVSTSTLNQQNSTIGNGIGNHSQVDITSSKVSSPFLQCDNSNSKQSTPTLEDLILQLEMEEEMARKEKLNEYSGIRGRMSCVNNSDILRSARNALNQYPRFSLDGRDSMYRSSFGTIEGRRSVCSEISLEPKLDEKVMCFPPTVAGENVVWCKPGVVAKLMGLEAIPVSVGRKRSDKKERMSSVDCRRQNIRRRPFERHDLERKLAMDMQGYRDHIVRRRNRSGFCSKNGYCIMKPVSLEAMAGGPSSWQPRRYV* |
