AlfalfaGEDB | Msa0659010

M. sativa cultivar zhongmu-4 / Msa0659010

KEGG: K07897|K07976
GO: -
PFAM: PF00071.25

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0659010	XP_003630119.1	98.086	209	4	0	1	209	1	209	5.30e-153	432

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0659010	sp\|Q43463\|RAB7_SOYBN	79.024	205	42	1	6	209	2	206	7.56e-122	346

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
No data available in table

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0003470	Msa0659010	0.826760	2.272851e-54	-8.615850e-47
Msa0004350	Msa0659010	0.825561	4.383584e-54	-8.615850e-47
Msa0008850	Msa0659010	0.832157	1.109489e-55	-8.615850e-47
Msa0011720	Msa0659010	0.813498	2.483134e-51	-8.615850e-47
Msa0017120	Msa0659010	0.821015	5.053480e-53	-8.615850e-47
Msa0024680	Msa0659010	0.814903	1.215581e-51	-8.615850e-47
Msa0027540	Msa0659010	0.835487	1.630576e-56	-8.615850e-47
Msa0028060	Msa0659010	0.802460	5.550563e-49	-8.615850e-47
Msa0030000	Msa0659010	0.820366	7.125573e-53	-8.615850e-47
Msa0030590	Msa0659010	0.813908	2.017004e-51	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0659010	MtrunA17_Chr8g0383261	98.086	209	4	0	1	209	1	209	4.87e-157	432
Msa0659010	MtrunA17_Chr6g0466301	76.847	203	46	1	8	209	4	206	1.13e-116	330
Msa0659010	MtrunA17_Chr6g0465491	76.847	203	46	1	8	209	4	206	1.13e-116	330
Msa0659010	MtrunA17_Chr1g0201121	74.757	206	50	1	6	209	2	207	1.59e-115	327
Msa0659010	MtrunA17_Chr2g0331691	65.700	207	71	0	3	209	1	207	1.78e-102	294
Msa0659010	MtrunA17_Chr4g0034871	67.488	203	65	1	8	209	4	206	1.02e-100	290
Msa0659010	MtrunA17_Chr4g0004661	66.337	202	68	0	8	209	60	261	1.39e-100	291
Msa0659010	MtrunA17_Chr6g0466161	65.193	181	63	0	29	209	7	187	4.11e-83	245
Msa0659010	MtrunA17_Chr6g0465471	64.641	181	64	0	29	209	7	187	1.82e-82	244
Msa0659010	MtrunA17_Chr6g0466431	62.617	107	31	1	8	114	4	101	5.13e-44	142

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0659010	AT1G22740.1	75.490	204	48	1	6	209	2	203	5.52e-113	321
Msa0659010	AT4G09720.1	71.429	203	57	1	8	209	4	206	4.25e-110	314
Msa0659010	AT4G09720.4	70.192	208	56	3	8	209	4	211	1.32e-106	305
Msa0659010	AT4G09720.3	67.757	214	57	2	8	209	4	217	2.21e-106	305
Msa0659010	AT2G21880.1	70.000	210	57	2	6	209	3	212	6.64e-103	296
Msa0659010	AT1G52280.1	67.317	205	66	1	6	209	2	206	9.97e-103	295
Msa0659010	AT3G16100.1	66.341	205	68	1	6	209	2	206	1.80e-101	292
Msa0659010	AT3G18820.1	67.488	203	65	1	8	209	4	206	3.82e-100	289
Msa0659010	AT1G49300.2	66.176	204	68	1	7	209	3	206	1.18e-99	288
Msa0659010	AT1G49300.1	66.176	204	68	1	7	209	3	206	1.18e-99	288

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Find 53 sgRNAs with CRISPR-Local

Find 274 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATGCAGCTCCAAGACTA+TGG	0.389043	4_3:-69258077	None:intergenic
AACAAAATCAGCCCCAATTG+TGG	0.543201	4_3:-69257873	None:intergenic
AACAAGGTTGATATAGATGG+TGG	0.570432	4_3:+69258702	Msa0659010:CDS
AACAGTATAAAGCCACAATT+GGG	0.408620	4_3:+69257861	Msa0659010:CDS
AAGGGGTTTCAGATATTCCA+CGG	0.600915	4_3:-69259112	None:intergenic
AATACAAAGGGAAATGCCTC+AGG	0.512303	4_3:-69258672	None:intergenic
AATTCTATTATCCGTAAGTA+TGG	0.278709	4_3:+69259279	Msa0659010:three_prime_UTR
AATTGATCTCAGGCAGATAC+AGG	0.484106	4_3:+69258645	Msa0659010:intron
ACAGTATAAAGCCACAATTG+GGG	0.725981	4_3:+69257862	Msa0659010:CDS
AGAAGGATTATCCATACTTA+CGG	0.530605	4_3:-69259290	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TGAACTAGAAATATCCATGT+TGG	-	chr4_3:69257150-69257169	None:intergenic	30.0%
GACATGAAAACATATGAAGA+AGG	-	chr4_3:69257237-69257256	None:intergenic	30.0%
AACAGTATAAAGCCACAATT+GGG	+	chr4_3:69257861-69257880	Msa0659010:CDS	30.0%
TTACAATGATATGGCAGATT+TGG	+	chr4_3:69258031-69258050	Msa0659010:intron	30.0%
TACAATGATATGGCAGATTT+GGG	+	chr4_3:69258032-69258051	Msa0659010:intron	30.0%
AAGATTGTAAGCCAAACAAA+GGG	-	chr4_3:69258269-69258288	None:intergenic	30.0%
TAAGATTGTAAGCCAAACAA+AGG	-	chr4_3:69258270-69258289	None:intergenic	30.0%
AATCATTGGATCAAATGGAA+CGG	+	chr4_3:69258345-69258364	Msa0659010:intron	30.0%
TTTATCTTCCGATAGAATCT+TGG	+	chr4_3:69258515-69258534	Msa0659010:intron	30.0%
TTTGATGTGAAATTGATCTC+AGG	+	chr4_3:69258635-69258654	Msa0659010:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_3	gene	69257111	69259439	69257111	ID=Msa0659010;Name=Msa0659010
chr4_3	mRNA	69257111	69259439	69257111	ID=Msa0659010-mRNA-1;Parent=Msa0659010;Name=Msa0659010-mRNA-1;_AED=0.14;_eAED=0.14;_QI=41\|1\|1\|1\|1\|1\|7\|270\|209
chr4_3	exon	69257111	69257216	69257111	ID=Msa0659010-mRNA-1:exon:20258;Parent=Msa0659010-mRNA-1
chr4_3	exon	69257335	69257361	69257335	ID=Msa0659010-mRNA-1:exon:20259;Parent=Msa0659010-mRNA-1
chr4_3	exon	69257838	69257937	69257838	ID=Msa0659010-mRNA-1:exon:20260;Parent=Msa0659010-mRNA-1
chr4_3	exon	69258048	69258194	69258048	ID=Msa0659010-mRNA-1:exon:20261;Parent=Msa0659010-mRNA-1
chr4_3	exon	69258657	69258737	69258657	ID=Msa0659010-mRNA-1:exon:20262;Parent=Msa0659010-mRNA-1
chr4_3	exon	69258846	69258991	69258846	ID=Msa0659010-mRNA-1:exon:20263;Parent=Msa0659010-mRNA-1
chr4_3	exon	69259106	69259439	69259106	ID=Msa0659010-mRNA-1:exon:20264;Parent=Msa0659010-mRNA-1
chr4_3	five_prime_UTR	69257111	69257151	69257111	ID=Msa0659010-mRNA-1:five_prime_utr;Parent=Msa0659010-mRNA-1
chr4_3	CDS	69257152	69257216	69257152	ID=Msa0659010-mRNA-1:cds;Parent=Msa0659010-mRNA-1
chr4_3	CDS	69257335	69257361	69257335	ID=Msa0659010-mRNA-1:cds;Parent=Msa0659010-mRNA-1
chr4_3	CDS	69257838	69257937	69257838	ID=Msa0659010-mRNA-1:cds;Parent=Msa0659010-mRNA-1
chr4_3	CDS	69258048	69258194	69258048	ID=Msa0659010-mRNA-1:cds;Parent=Msa0659010-mRNA-1
chr4_3	CDS	69258657	69258737	69258657	ID=Msa0659010-mRNA-1:cds;Parent=Msa0659010-mRNA-1
chr4_3	CDS	69258846	69258991	69258846	ID=Msa0659010-mRNA-1:cds;Parent=Msa0659010-mRNA-1
chr4_3	CDS	69259106	69259169	69259106	ID=Msa0659010-mRNA-1:cds;Parent=Msa0659010-mRNA-1
chr4_3	three_prime_UTR	69259170	69259439	69259170	ID=Msa0659010-mRNA-1:three_prime_utr;Parent=Msa0659010-mRNA-1

Gene Sequence

>Msa0659010

ATGGATATTTCTAGTTCACACAGAAAGAGAACTTTGCTTAAGGTCATTGTCCTTGGAGATAGTGGGGTTGGGAAGACATCTTTGATGAACCAATATGTGTATAAAAAATTTAGCCAACAGTATAAAGCCACAATTGGGGCTGATTTTGTTACAAAGGAGCTGCTAGTTGATGATAAACTCGTAACCTTGCAGATTTGGGATACTGCAGGACAGGAAAGGTTCCATAGTCTTGGAGCTGCATTTTACAGAGGGGCAGATTGTTGTGTTTTGGTATATGATGTAAATATACACAAAACATTTGATACATTAAACAATTGGCATGATGACTTTCTTAAGCAGGCAGATACAGGCAATCCTGAGGCATTTCCCTTTGTATTACTTGGGAACAAGGTTGATATAGATGGTGGAAACAGTAGAAGGGTTACAGAGAAGAAAGCTAGGGAATGGTGTGCTTCTAGGGGTAACATACCATATTTTGAAACCTCTGCAAAAGAGGGCTACAATGTTGATGATGCATTTTTATGTGTTGCTAAAGTTGCATCAGAAAATGAACACGATTTAGACATTTATTTCCGTGGAATATCTGAAACCCCTTCAGAAGCAGAACAACGAAGTAATTGTGCATGCTGA

Protein sequence

>Msa0659010

MDISSSHRKRTLLKVIVLGDSGVGKTSLMNQYVYKKFSQQYKATIGADFVTKELLVDDKLVTLQIWDTAGQERFHSLGAAFYRGADCCVLVYDVNIHKTFDTLNNWHDDFLKQADTGNPEAFPFVLLGNKVDIDGGNSRRVTEKKAREWCASRGNIPYFETSAKEGYNVDDAFLCVAKVASENEHDLDIYFRGISETPSEAEQRSNCAC*