AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0659010

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0659010 MtrunA17_Chr8g0383261 98.086 209 4 0 1 209 1 209 4.87e-157 432
Msa0659010 MtrunA17_Chr6g0466301 76.847 203 46 1 8 209 4 206 1.13e-116 330
Msa0659010 MtrunA17_Chr6g0465491 76.847 203 46 1 8 209 4 206 1.13e-116 330
Msa0659010 MtrunA17_Chr1g0201121 74.757 206 50 1 6 209 2 207 1.59e-115 327
Msa0659010 MtrunA17_Chr2g0331691 65.700 207 71 0 3 209 1 207 1.78e-102 294
Msa0659010 MtrunA17_Chr4g0034871 67.488 203 65 1 8 209 4 206 1.02e-100 290
Msa0659010 MtrunA17_Chr4g0004661 66.337 202 68 0 8 209 60 261 1.39e-100 291
Msa0659010 MtrunA17_Chr6g0466161 65.193 181 63 0 29 209 7 187 4.11e-83 245
Msa0659010 MtrunA17_Chr6g0465471 64.641 181 64 0 29 209 7 187 1.82e-82 244
Msa0659010 MtrunA17_Chr6g0466431 62.617 107 31 1 8 114 4 101 5.13e-44 142
Msa0659010 MtrunA17_Chr6g0465611 62.617 107 31 1 8 114 4 101 5.13e-44 142
Msa0659010 MtrunA17_Chr4g0040781 42.593 162 86 3 12 173 13 167 1.52e-38 132
Msa0659010 MtrunA17_Chr5g0400131 37.019 208 108 6 14 209 11 207 1.92e-38 132
Msa0659010 MtrunA17_Chr1g0174281 42.424 165 88 3 12 176 15 172 2.31e-38 132
Msa0659010 MtrunA17_Chr3g0109721 36.598 194 116 4 14 207 12 198 4.71e-38 130
Msa0659010 MtrunA17_Chr2g0283131 41.975 162 87 3 12 173 13 167 8.99e-38 130
Msa0659010 MtrunA17_Chr4g0046261 41.975 162 87 3 12 173 13 167 1.20e-37 130
Msa0659010 MtrunA17_Chr7g0214241 41.317 167 91 3 12 178 15 174 1.21e-37 130
Msa0659010 MtrunA17_Chr8g0351861 41.718 163 88 3 12 174 16 171 1.33e-37 130
Msa0659010 MtrunA17_Chr2g0327911 40.120 167 95 3 12 178 15 176 3.85e-37 128
Msa0659010 MtrunA17_Chr5g0433561 36.082 194 117 4 14 207 12 198 1.04e-36 127
Msa0659010 MtrunA17_Chr7g0231131 41.212 165 90 3 12 176 15 172 1.78e-36 127
Msa0659010 MtrunA17_Chr8g0381841 37.791 172 100 3 5 176 10 174 1.96e-36 127
Msa0659010 MtrunA17_Chr3g0123971 33.498 203 121 4 14 209 11 206 3.88e-36 125
Msa0659010 MtrunA17_Chr1g0182301 39.881 168 93 3 12 178 15 175 4.04e-36 126
Msa0659010 MtrunA17_Chr2g0325091 40.606 165 91 3 12 176 11 168 4.24e-36 126
Msa0659010 MtrunA17_Chr1g0212521 35.437 206 116 4 12 207 15 213 4.24e-36 126
Msa0659010 MtrunA17_Chr8g0382221 33.824 204 120 4 14 209 11 207 4.88e-36 125
Msa0659010 MtrunA17_Chr6g0458081 38.857 175 100 3 12 186 6 173 5.04e-36 125
Msa0659010 MtrunA17_Chr2g0276941 40.123 162 90 3 12 173 15 169 5.80e-36 125
Msa0659010 MtrunA17_Chr4g0032201 36.471 170 101 2 7 176 7 169 5.98e-36 125
Msa0659010 MtrunA17_Chr2g0315551 34.848 198 112 4 12 209 13 193 1.24e-35 125
Msa0659010 MtrunA17_Chr4g0030911 37.073 205 113 5 12 207 15 212 1.32e-35 124
Msa0659010 MtrunA17_Chr1g0182321 39.286 168 94 3 12 178 15 175 1.39e-35 124
Msa0659010 MtrunA17_Chr7g0263071 38.202 178 102 3 12 188 15 185 1.86e-35 124
Msa0659010 MtrunA17_Chr3g0123821 38.182 165 95 3 12 176 16 173 1.95e-35 124
Msa0659010 MtrunA17_Chr4g0055081 35.096 208 111 7 14 209 11 206 5.02e-35 123
Msa0659010 MtrunA17_Chr8g0390561 37.356 174 102 4 5 178 2 168 1.03e-34 122
Msa0659010 MtrunA17_Chr3g0112681 36.364 165 98 2 12 176 12 169 1.97e-34 121
Msa0659010 MtrunA17_Chr7g0234201 37.725 167 97 3 12 178 13 172 3.78e-34 120
Msa0659010 MtrunA17_Chr8g0335471 38.554 166 95 3 12 177 8 166 5.26e-34 120
Msa0659010 MtrunA17_Chr2g0290891 37.952 166 96 2 12 177 6 164 5.29e-34 120
Msa0659010 MtrunA17_Chr4g0055651 37.349 166 97 2 12 177 8 166 1.10e-33 119
Msa0659010 MtrunA17_Chr2g0319951 38.889 162 92 2 12 173 13 167 1.27e-33 119
Msa0659010 MtrunA17_Chr7g0250531 37.952 166 96 3 12 177 8 166 3.70e-33 118
Msa0659010 MtrunA17_Chr7g0275991 38.650 163 93 3 12 174 20 175 5.86e-33 118
Msa0659010 MtrunA17_Chr1g0189361 37.725 167 97 2 12 178 14 173 7.56e-33 117
Msa0659010 MtrunA17_Chr8g0346421 34.579 214 117 6 12 209 26 232 6.30e-32 115
Msa0659010 MtrunA17_Chr2g0276931 36.416 173 103 3 5 177 2 167 7.67e-32 114
Msa0659010 MtrunA17_Chr6g0482721 39.264 163 92 4 12 174 12 167 9.38e-32 114
Msa0659010 MtrunA17_Chr8g0349711 35.088 171 104 2 13 183 7 170 4.46e-31 113
Msa0659010 MtrunA17_Chr8g0349681 37.427 171 100 2 13 183 176 339 1.71e-30 117
Msa0659010 MtrunA17_Chr8g0349681 35.404 161 97 3 13 173 14 167 2.53e-26 105
Msa0659010 MtrunA17_Chr8g0349681 32.584 178 113 2 12 189 384 554 5.83e-26 104
Msa0659010 MtrunA17_Chr8g0392931 38.037 163 94 3 12 174 14 169 3.90e-30 110
Msa0659010 MtrunA17_Chr1g0199331 34.503 171 104 5 9 178 28 191 8.59e-28 104
Msa0659010 MtrunA17_Chr7g0265001 38.509 161 92 5 14 173 18 172 7.85e-27 102
Msa0659010 MtrunA17_Chr1g0159481 39.634 164 89 6 12 173 17 172 8.74e-27 102
Msa0659010 MtrunA17_Chr8g0384311 38.068 176 98 7 1 173 1 168 1.29e-26 101
Msa0659010 MtrunA17_Chr3g0138121 31.325 166 104 3 13 178 14 169 2.30e-26 100
Msa0659010 MtrunA17_Chr3g0138101 31.325 166 104 3 13 178 14 169 2.32e-26 100
Msa0659010 MtrunA17_Chr3g0138111 31.325 166 104 3 13 178 14 169 2.64e-26 100
Msa0659010 MtrunA17_Chr3g0130271 37.143 175 101 6 1 173 1 168 1.82e-25 98.2
Msa0659010 MtrunA17_Chr1g0180291 34.653 202 120 6 13 209 15 209 5.74e-25 97.1
Msa0659010 MtrunA17_Chr6g0466421 58.108 74 28 1 116 186 14 87 1.45e-24 92.8
Msa0659010 MtrunA17_Chr6g0465601 64.062 64 23 0 123 186 14 77 1.65e-24 92.0
Msa0659010 MtrunA17_Chr1g0197331 35.821 201 117 6 13 207 16 210 7.37e-24 94.4
Msa0659010 MtrunA17_Chr6g0486041 28.986 207 123 6 12 208 6 198 4.65e-18 79.0
Msa0659010 MtrunA17_Chr2g0325101 29.944 177 109 4 12 179 6 176 5.32e-18 78.6
Msa0659010 MtrunA17_Chr2g0326141 32.105 190 100 8 23 189 172 355 1.32e-16 77.0
Msa0659010 MtrunA17_Chr5g0405791 28.824 170 108 3 12 173 6 170 4.30e-16 73.6
Msa0659010 MtrunA17_Chr4g0037021 28.249 177 112 4 12 179 6 176 4.77e-16 73.2
Msa0659010 MtrunA17_Chr3g0117931 29.825 171 105 4 12 173 8 172 1.44e-15 72.4
Msa0659010 MtrunA17_Chr8g0371501 29.412 170 107 4 12 173 6 170 2.39e-15 71.2
Msa0659010 MtrunA17_Chr4g0046211 29.861 144 86 4 12 146 6 143 1.89e-14 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0659010 AT1G22740.1 75.490 204 48 1 6 209 2 203 5.52e-113 321
Msa0659010 AT4G09720.1 71.429 203 57 1 8 209 4 206 4.25e-110 314
Msa0659010 AT4G09720.4 70.192 208 56 3 8 209 4 211 1.32e-106 305
Msa0659010 AT4G09720.3 67.757 214 57 2 8 209 4 217 2.21e-106 305
Msa0659010 AT2G21880.1 70.000 210 57 2 6 209 3 212 6.64e-103 296
Msa0659010 AT1G52280.1 67.317 205 66 1 6 209 2 206 9.97e-103 295
Msa0659010 AT3G16100.1 66.341 205 68 1 6 209 2 206 1.80e-101 292
Msa0659010 AT3G18820.1 67.488 203 65 1 8 209 4 206 3.82e-100 289
Msa0659010 AT1G49300.2 66.176 204 68 1 7 209 3 206 1.18e-99 288
Msa0659010 AT1G49300.1 66.176 204 68 1 7 209 3 206 1.18e-99 288
Msa0659010 AT2G21880.2 66.190 210 57 3 6 209 3 204 4.29e-93 271
Msa0659010 AT2G21880.4 67.914 187 54 2 29 209 1 187 2.14e-88 258
Msa0659010 AT2G21880.3 67.914 187 54 2 29 209 1 187 2.14e-88 258
Msa0659010 AT4G09720.6 67.836 171 54 1 40 209 2 172 2.89e-87 255
Msa0659010 AT4G09720.5 67.836 171 54 1 40 209 2 172 2.89e-87 255
Msa0659010 AT4G09720.2 67.836 171 54 1 40 209 2 172 2.89e-87 255
Msa0659010 AT5G39620.1 53.695 203 92 1 9 209 2 204 8.88e-77 229
Msa0659010 AT3G12160.1 43.030 165 87 3 12 176 15 172 3.89e-39 134
Msa0659010 AT3G15060.1 41.111 180 95 4 12 187 13 185 1.44e-38 132
Msa0659010 AT5G60860.1 41.975 162 87 3 12 173 13 167 2.99e-38 132
Msa0659010 AT1G28550.1 41.975 162 87 3 12 173 13 167 5.21e-38 131
Msa0659010 AT2G33870.1 40.606 165 91 3 12 176 13 170 8.74e-38 130
Msa0659010 AT2G44610.1 36.364 209 109 6 14 209 11 208 1.80e-37 129
Msa0659010 AT5G10260.1 35.096 208 112 5 14 209 11 207 3.68e-37 129
Msa0659010 AT5G45750.1 39.655 174 95 3 12 185 13 176 7.45e-37 128
Msa0659010 AT5G47960.1 40.606 165 91 3 12 176 15 172 1.09e-36 128
Msa0659010 AT5G59840.1 39.326 178 100 3 12 188 15 185 1.13e-36 127
Msa0659010 AT3G53610.2 37.853 177 104 2 12 188 15 185 1.38e-36 127
Msa0659010 AT3G53610.3 37.853 177 104 2 12 188 15 185 1.38e-36 127
Msa0659010 AT3G53610.1 37.853 177 104 2 12 188 15 185 1.38e-36 127
Msa0659010 AT4G18430.1 38.788 165 94 3 12 176 13 170 3.38e-36 126
Msa0659010 AT1G05810.1 38.798 183 96 3 8 190 8 174 6.68e-36 125
Msa0659010 AT1G09630.1 38.788 165 94 3 12 176 12 169 7.56e-36 125
Msa0659010 AT2G43130.1 38.922 167 95 3 12 178 12 171 7.78e-36 125
Msa0659010 AT1G73640.1 38.043 184 98 5 12 186 13 189 1.12e-35 125
Msa0659010 AT5G59150.1 38.788 165 94 3 12 176 12 169 1.24e-35 125
Msa0659010 AT1G05810.2 38.798 183 96 3 8 190 54 220 1.84e-35 126
Msa0659010 AT5G45130.1 38.636 176 101 4 3 178 1 169 3.06e-35 124
Msa0659010 AT2G31680.1 38.323 167 96 2 12 178 12 171 5.06e-35 124
Msa0659010 AT3G46060.2 37.640 178 103 3 12 188 15 185 5.86e-35 123
Msa0659010 AT3G46060.3 37.640 178 103 3 12 188 15 185 5.86e-35 123
Msa0659010 AT3G46060.1 37.640 178 103 3 12 188 15 185 5.86e-35 123
Msa0659010 AT5G64990.1 37.321 209 107 8 14 209 9 206 8.50e-35 122
Msa0659010 AT5G65270.1 39.394 165 93 3 12 176 17 174 8.64e-35 123
Msa0659010 AT4G18800.1 38.889 162 92 2 12 173 13 167 1.08e-34 122
Msa0659010 AT2G22290.1 33.981 206 117 5 14 209 11 207 2.18e-34 121
Msa0659010 AT1G07410.1 35.882 170 102 3 7 176 7 169 2.88e-34 121
Msa0659010 AT4G35860.1 37.143 175 103 4 12 186 6 173 2.96e-34 121
Msa0659010 AT1G18200.1 39.394 165 93 4 12 176 13 170 4.46e-34 121
Msa0659010 AT1G16920.1 40.123 162 90 3 12 173 13 167 7.47e-34 120
Msa0659010 AT1G06400.1 38.889 162 92 2 12 173 13 167 7.72e-34 120
Msa0659010 AT4G17170.1 37.952 166 96 3 12 177 6 164 9.98e-34 120
Msa0659010 AT3G09900.1 36.683 199 118 3 12 208 15 207 1.03e-33 120
Msa0659010 AT4G19640.1 36.932 176 104 4 3 178 1 169 1.04e-33 119
Msa0659010 AT4G39990.1 37.654 162 94 3 12 173 17 171 1.08e-33 120
Msa0659010 AT4G39890.1 37.870 169 91 4 13 177 10 168 1.16e-33 120
Msa0659010 AT3G46830.1 36.364 165 98 3 12 176 12 169 1.71e-33 119
Msa0659010 AT3G46830.2 36.364 165 98 3 12 176 12 169 1.71e-33 119
Msa0659010 AT5G45130.2 39.264 163 92 4 16 178 22 177 6.87e-33 117
Msa0659010 AT4G17160.1 34.314 204 118 5 13 207 7 203 7.15e-33 117
Msa0659010 AT1G01200.1 38.650 163 94 3 12 174 28 184 7.17e-33 118
Msa0659010 AT1G02130.1 36.257 171 102 3 12 182 8 171 1.03e-32 117
Msa0659010 AT5G03520.1 38.542 192 100 5 12 202 15 189 1.13e-32 117
Msa0659010 AT3G07410.1 38.323 167 96 2 12 178 12 171 1.79e-32 117
Msa0659010 AT4G17530.1 36.970 165 97 2 12 176 8 165 1.96e-32 116
Msa0659010 AT5G47200.1 36.970 165 97 2 12 176 8 165 3.49e-32 115
Msa0659010 AT5G64990.2 36.111 216 107 9 14 209 9 213 4.11e-32 116
Msa0659010 AT3G11730.1 37.952 166 96 3 12 177 8 166 5.92e-32 115
Msa0659010 AT5G47520.1 35.329 167 101 3 12 178 14 173 1.37e-30 112
Msa0659010 AT4G19640.2 38.462 143 82 3 3 145 1 137 9.56e-29 105
Msa0659010 AT3G54840.2 35.714 168 100 5 9 175 30 190 1.79e-28 106
Msa0659010 AT4G19640.3 33.708 178 108 6 14 191 12 179 4.30e-28 105
Msa0659010 AT3G54840.1 35.088 171 103 5 9 178 30 193 8.07e-28 104
Msa0659010 AT3G54840.3 35.152 165 99 5 15 178 2 159 3.99e-27 102
Msa0659010 AT1G43890.3 39.634 164 89 6 12 173 13 168 5.29e-27 102
Msa0659010 AT1G43890.1 39.634 164 89 6 12 173 13 168 5.29e-27 102
Msa0659010 AT1G43890.2 39.634 164 89 6 12 173 13 168 5.29e-27 102
Msa0659010 AT5G03530.2 34.826 201 119 6 14 209 15 208 9.55e-27 102
Msa0659010 AT5G03530.1 34.826 201 119 6 14 209 15 208 9.55e-27 102
Msa0659010 AT5G20010.1 30.688 189 115 5 13 201 14 186 2.73e-26 101
Msa0659010 AT5G55190.1 31.325 166 104 3 13 178 14 169 3.34e-26 100
Msa0659010 AT5G20020.1 31.325 166 104 3 13 178 14 169 3.75e-26 100
Msa0659010 AT5G03520.2 38.462 156 78 5 48 202 41 179 2.99e-25 98.2
Msa0659010 AT5G55080.1 28.994 169 104 4 13 178 14 169 3.03e-23 93.2
Msa0659010 AT4G35860.2 36.090 133 78 4 54 186 2 127 7.84e-22 88.2
Msa0659010 AT5G59150.2 38.017 121 68 3 56 176 5 118 2.49e-20 84.3
Msa0659010 AT3G09910.3 35.185 162 97 6 13 173 14 168 3.63e-19 82.0
Msa0659010 AT3G09910.1 35.185 162 97 6 13 173 14 168 3.63e-19 82.0
Msa0659010 AT3G09910.2 35.185 162 97 6 13 173 14 168 3.63e-19 82.0
Msa0659010 AT3G09910.5 34.286 175 107 5 13 186 14 181 4.23e-19 81.6
Msa0659010 AT3G09910.4 34.286 175 107 5 13 186 14 181 4.23e-19 81.6
Msa0659010 AT4G28950.1 28.922 204 126 6 12 208 6 197 1.84e-18 80.5
Msa0659010 AT5G45970.1 29.944 177 111 4 12 180 6 177 1.96e-18 80.1
Msa0659010 AT2G44690.1 29.825 171 105 4 12 173 18 182 4.38e-17 76.6
Msa0659010 AT1G20090.1 28.652 178 112 4 12 180 5 176 1.13e-16 75.1
Msa0659010 AT1G75840.1 28.652 178 112 4 12 180 6 177 1.68e-16 74.7
Msa0659010 AT3G51300.1 30.000 170 106 3 12 173 6 170 1.98e-16 74.7
Msa0659010 AT2G17800.2 30.000 170 106 3 12 173 6 170 2.54e-16 74.3
Msa0659010 AT2G17800.1 30.000 170 106 3 12 173 6 170 2.54e-16 74.3
Msa0659010 AT4G35950.1 30.000 170 106 3 12 173 6 170 3.22e-16 73.9
Msa0659010 AT4G35020.1 27.684 177 113 4 12 179 6 176 1.18e-15 72.8
Msa0659010 AT4G35020.2 27.684 177 113 4 12 179 6 176 1.18e-15 72.8
Msa0659010 AT4G35020.3 27.684 177 113 4 12 179 6 176 1.18e-15 72.8
Msa0659010 AT3G48040.1 29.775 178 110 4 12 180 8 179 1.57e-15 72.4
Msa0659010 AT5G62880.1 29.825 171 105 4 12 173 8 172 3.33e-15 71.6
Msa0659010 AT3G48040.2 29.444 180 110 5 12 182 8 179 7.06e-15 70.1
Msa0659010 AT5G62880.2 29.480 173 107 4 12 175 8 174 1.04e-14 70.1
Msa0659010 AT4G35020.4 29.752 121 78 3 12 131 6 120 2.42e-12 62.4
Msa0659010 AT4G35020.5 29.752 121 78 3 12 131 6 120 2.42e-12 62.4

Find 53 sgRNAs with CRISPR-Local

Find 274 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATGCTGAAGTTGTCGTTTA+AGG 0.202463 4_3:+69259162 Msa0659010:exon
TTTGCAGAGGTTTCAAAATA+TGG 0.212498 4_3:-69258894 None:intergenic
CTAGGGAATGGTGTGCTTCT+AGG 0.247665 4_3:+69258862 Msa0659010:CDS
AATTCTATTATCCGTAAGTA+TGG 0.278709 4_3:+69259279 Msa0659010:three_prime_UTR
GTTCAAATTGTGTTTGGTTC+AGG 0.281773 4_3:+69259242 Msa0659010:three_prime_UTR
GCATTTCCCTTTGTATTACT+TGG 0.328430 4_3:+69258678 Msa0659010:CDS
AGGGGCAGATTGTTGTGTTT+TGG 0.341202 4_3:+69258104 Msa0659010:CDS
GTCATTGTCCTTGGAGATAG+TGG 0.363454 4_3:+69257194 Msa0659010:CDS
TTAGTTGTTCAAATTGTGTT+TGG 0.367616 4_3:+69259236 Msa0659010:three_prime_UTR
TGATAAACTCGTAACCTTGC+AGG 0.369262 4_3:+69257916 Msa0659010:CDS
CAACAGTATAAAGCCACAAT+TGG 0.380292 4_3:+69257860 Msa0659010:CDS
CATTTGATACATTAAACAAT+TGG 0.385874 4_3:+69258151 Msa0659010:CDS
TAGATGGTGGAAACAGTAGA+AGG 0.386136 4_3:+69258715 Msa0659010:CDS
AAAATGCAGCTCCAAGACTA+TGG 0.389043 4_3:-69258077 None:intergenic
TGTGTTTGGTTCAGGTTCAA+TGG 0.392256 4_3:+69259250 Msa0659010:three_prime_UTR
TTGCTTAAGGTCATTGTCCT+TGG 0.394981 4_3:+69257185 Msa0659010:CDS
AACAGTATAAAGCCACAATT+GGG 0.408620 4_3:+69257861 Msa0659010:CDS
TAGGGAATGGTGTGCTTCTA+GGG 0.415556 4_3:+69258863 Msa0659010:CDS
GTGTTTGGTTCAGGTTCAAT+GGG 0.415852 4_3:+69259251 Msa0659010:three_prime_UTR
CAGAAAGAGAACTTTGCTTA+AGG 0.420667 4_3:+69257172 Msa0659010:CDS
CAATTGTGGCTTTATACTGT+TGG 0.421726 4_3:-69257859 None:intergenic
CTTTGTATTACTTGGGAACA+AGG 0.440726 4_3:+69258686 Msa0659010:CDS
CTTCGTTGTTCTGCTTCTGA+AGG 0.452987 4_3:-69259131 None:intergenic
TTCGTTGTTCTGCTTCTGAA+GGG 0.456355 4_3:-69259130 None:intergenic
GGGAACAAGGTTGATATAGA+TGG 0.470323 4_3:+69258699 Msa0659010:CDS
CAGGAAAGGTTCCATAGTCT+TGG 0.475873 4_3:+69258066 Msa0659010:CDS
GCATGATGACTTTCTTAAGC+AGG 0.480067 4_3:+69258173 Msa0659010:CDS
AATTGATCTCAGGCAGATAC+AGG 0.484106 4_3:+69258645 Msa0659010:intron
CATTTCCCTTTGTATTACTT+GGG 0.485534 4_3:+69258679 Msa0659010:CDS
AGGTTACAGAGAAGAAAGCT+AGG 0.511353 4_3:+69258844 Msa0659010:intron
AATACAAAGGGAAATGCCTC+AGG 0.512303 4_3:-69258672 None:intergenic
TTTAAAATTACTCACCTGCA+AGG 0.529499 4_3:-69257930 None:intergenic
AGAAGGATTATCCATACTTA+CGG 0.530605 4_3:-69259290 None:intergenic
TTTGAAACCTCTGCAAAAGA+GGG 0.539597 4_3:+69258900 Msa0659010:CDS
GATTTGGGATACTGCAGGAC+AGG 0.541113 4_3:+69258047 Msa0659010:intron
AACAAAATCAGCCCCAATTG+TGG 0.543201 4_3:-69257873 None:intergenic
CTTGTTCCCAAGTAATACAA+AGG 0.556095 4_3:-69258685 None:intergenic
TTTCTTGGCAGTTATTTCCG+TGG 0.558899 4_3:+69259095 Msa0659010:intron
AACAAGGTTGATATAGATGG+TGG 0.570432 4_3:+69258702 Msa0659010:CDS
CAGAGAAGAAAGCTAGGGAA+TGG 0.574138 4_3:+69258850 Msa0659010:CDS
TTGTTCCCAAGTAATACAAA+GGG 0.581722 4_3:-69258684 None:intergenic
TCGTTGTTCTGCTTCTGAAG+GGG 0.584150 4_3:-69259129 None:intergenic
TGGCAGATTTGGGATACTGC+AGG 0.586660 4_3:+69258042 Msa0659010:intron
GGGATACTGCAGGACAGGAA+AGG 0.587274 4_3:+69258052 Msa0659010:CDS
AAGGGGTTTCAGATATTCCA+CGG 0.600915 4_3:-69259112 None:intergenic
TGAACTAGAAATATCCATGT+TGG 0.610654 4_3:-69257147 None:intergenic
GGTTACAGAGAAGAAAGCTA+GGG 0.611053 4_3:+69258845 Msa0659010:intron
TCATTGTCCTTGGAGATAGT+GGG 0.622102 4_3:+69257195 Msa0659010:CDS
AGATGGTGGAAACAGTAGAA+GGG 0.623050 4_3:+69258716 Msa0659010:CDS
TAACATACCCACTATCTCCA+AGG 0.630039 4_3:-69257202 None:intergenic
GGCAGATACAGGCAATCCTG+AGG 0.655656 4_3:+69258656 Msa0659010:intron
AGGGAATGGTGTGCTTCTAG+GGG 0.665067 4_3:+69258864 Msa0659010:CDS
ACAGTATAAAGCCACAATTG+GGG 0.725981 4_3:+69257862 Msa0659010:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTATAGTACTTTTATTTTT+AGG + chr4_3:69257987-69258006 Msa0659010:intron 10.0%
!!! TTATAGTACTTTTATTTTTA+GGG + chr4_3:69257988-69258007 Msa0659010:intron 10.0%
!!! ATTATAGTACTTTTATTTTT+AGG + chr4_3:69257987-69258006 Msa0659010:intron 10.0%
!!! TTATAGTACTTTTATTTTTA+GGG + chr4_3:69257988-69258007 Msa0659010:intron 10.0%
!!! TTCCAAATGATTAATTTTTT+TGG + chr4_3:69257457-69257476 Msa0659010:intron 15.0%
!! ATTTACATTATCCTAAAAAA+AGG - chr4_3:69257619-69257638 None:intergenic 15.0%
!! CAATTATAGAAAATATATTG+TGG + chr4_3:69257795-69257814 Msa0659010:intron 15.0%
!!! TATAGTACTTTTATTTTTAG+GGG + chr4_3:69257989-69258008 Msa0659010:intron 15.0%
!! AACAAAAATTTACAATGATA+TGG + chr4_3:69258022-69258041 Msa0659010:intron 15.0%
!!! TTCCAAATGATTAATTTTTT+TGG + chr4_3:69257457-69257476 Msa0659010:intron 15.0%
!! ATTTACATTATCCTAAAAAA+AGG - chr4_3:69257619-69257638 None:intergenic 15.0%
!! CAATTATAGAAAATATATTG+TGG + chr4_3:69257795-69257814 Msa0659010:intron 15.0%
!!! TATAGTACTTTTATTTTTAG+GGG + chr4_3:69257989-69258008 Msa0659010:intron 15.0%
!! AACAAAAATTTACAATGATA+TGG + chr4_3:69258022-69258041 Msa0659010:intron 15.0%
!! ATGATGTTATTATTACTTGA+TGG + chr4_3:69257263-69257282 Msa0659010:intron 20.0%
!!! AAATTCTGATATTTTGTTTC+AGG + chr4_3:69257313-69257332 Msa0659010:intron 20.0%
!!! AATTCTGATATTTTGTTTCA+GGG + chr4_3:69257314-69257333 Msa0659010:intron 20.0%
!! CTCCAAAAAAATTAATCATT+TGG - chr4_3:69257462-69257481 None:intergenic 20.0%
!! AAAAAAGGAACAAAACAAAA+GGG - chr4_3:69257499-69257518 None:intergenic 20.0%
!! TAAAAAAGGAACAAAACAAA+AGG - chr4_3:69257500-69257519 None:intergenic 20.0%
!!! AAATTTGGTCGTTTTAAAAA+AGG - chr4_3:69257514-69257533 None:intergenic 20.0%
!!! TTTTTTAAAACGACCAAATT+TGG + chr4_3:69257513-69257532 Msa0659010:intron 20.0%
!! CATTTGATACATTAAACAAT+TGG + chr4_3:69258151-69258170 Msa0659010:CDS 20.0%
!!! AGAGAAATGTTTAAAATCAT+TGG + chr4_3:69258331-69258350 Msa0659010:intron 20.0%
!!! TTTAAAATCATTGGATCAAA+TGG + chr4_3:69258340-69258359 Msa0659010:intron 20.0%
!! AAAAATTGCATACTCAAAAT+CGG - chr4_3:69258588-69258607 None:intergenic 20.0%
!!! TAACTTTCTTTTTTCTTGTT+AGG + chr4_3:69259001-69259020 Msa0659010:intron 20.0%
!! TAAGTTCACACAAAAAAATT+AGG + chr4_3:69259028-69259047 Msa0659010:intron 20.0%
!!! GGATGAATGAATAAATTTTA+AGG + chr4_3:69259183-69259202 Msa0659010:three_prime_UTR 20.0%
!!! ATATTTTATGCATTACATTC+AGG + chr4_3:69259398-69259417 Msa0659010:three_prime_UTR 20.0%
!! ATGATGTTATTATTACTTGA+TGG + chr4_3:69257263-69257282 Msa0659010:intron 20.0%
!!! AAATTCTGATATTTTGTTTC+AGG + chr4_3:69257313-69257332 Msa0659010:intron 20.0%
!!! AATTCTGATATTTTGTTTCA+GGG + chr4_3:69257314-69257333 Msa0659010:intron 20.0%
!! CTCCAAAAAAATTAATCATT+TGG - chr4_3:69257462-69257481 None:intergenic 20.0%
!! AAAAAAGGAACAAAACAAAA+GGG - chr4_3:69257499-69257518 None:intergenic 20.0%
!! TAAAAAAGGAACAAAACAAA+AGG - chr4_3:69257500-69257519 None:intergenic 20.0%
!!! AAATTTGGTCGTTTTAAAAA+AGG - chr4_3:69257514-69257533 None:intergenic 20.0%
!!! TTTTTTAAAACGACCAAATT+TGG + chr4_3:69257513-69257532 Msa0659010:intron 20.0%
!! CATTTGATACATTAAACAAT+TGG + chr4_3:69258151-69258170 Msa0659010:CDS 20.0%
!!! AGAGAAATGTTTAAAATCAT+TGG + chr4_3:69258331-69258350 Msa0659010:intron 20.0%
!!! TTTAAAATCATTGGATCAAA+TGG + chr4_3:69258340-69258359 Msa0659010:intron 20.0%
!! AAAAATTGCATACTCAAAAT+CGG - chr4_3:69258588-69258607 None:intergenic 20.0%
!!! TAACTTTCTTTTTTCTTGTT+AGG + chr4_3:69259001-69259020 Msa0659010:intron 20.0%
!! TAAGTTCACACAAAAAAATT+AGG + chr4_3:69259028-69259047 Msa0659010:intron 20.0%
!!! GGATGAATGAATAAATTTTA+AGG + chr4_3:69259183-69259202 Msa0659010:three_prime_UTR 20.0%
!!! ATATTTTATGCATTACATTC+AGG + chr4_3:69259398-69259417 Msa0659010:three_prime_UTR 20.0%
!!! GTTTTATTGAAAATGCCAAA+TGG - chr4_3:69257369-69257388 None:intergenic 25.0%
!!! GATCTTAAGTGATTTGTTTT+AGG + chr4_3:69257423-69257442 Msa0659010:intron 25.0%
! ATCCTAAAAAAAGGATCTAT+TGG - chr4_3:69257610-69257629 None:intergenic 25.0%
!! TAGATTGCATGTAAATTTTG+TGG - chr4_3:69257702-69257721 None:intergenic 25.0%
! ATCTAAACATATGCTGAAAT+TGG + chr4_3:69257717-69257736 Msa0659010:intron 25.0%
! ATATGCTGAAATTGGTTATA+CGG + chr4_3:69257725-69257744 Msa0659010:intron 25.0%
! AAATTATGGTATCTCACATT+TGG + chr4_3:69257768-69257787 Msa0659010:intron 25.0%
! AACTTGTACAAATAGAAAAG+TGG - chr4_3:69258239-69258258 None:intergenic 25.0%
! ATAAAAAATCCAAATCCTGT+TGG + chr4_3:69259057-69259076 Msa0659010:intron 25.0%
!!! TGACCAATATTGTTATTTCT+TGG + chr4_3:69259080-69259099 Msa0659010:intron 25.0%
! TTAGTTGTTCAAATTGTGTT+TGG + chr4_3:69259236-69259255 Msa0659010:three_prime_UTR 25.0%
! AATTCTATTATCCGTAAGTA+TGG + chr4_3:69259279-69259298 Msa0659010:three_prime_UTR 25.0%
!!! GTTTTATTGAAAATGCCAAA+TGG - chr4_3:69257369-69257388 None:intergenic 25.0%
!!! GATCTTAAGTGATTTGTTTT+AGG + chr4_3:69257423-69257442 Msa0659010:intron 25.0%
! ATCCTAAAAAAAGGATCTAT+TGG - chr4_3:69257610-69257629 None:intergenic 25.0%
!! TAGATTGCATGTAAATTTTG+TGG - chr4_3:69257702-69257721 None:intergenic 25.0%
! ATCTAAACATATGCTGAAAT+TGG + chr4_3:69257717-69257736 Msa0659010:intron 25.0%
! ATATGCTGAAATTGGTTATA+CGG + chr4_3:69257725-69257744 Msa0659010:intron 25.0%
! AAATTATGGTATCTCACATT+TGG + chr4_3:69257768-69257787 Msa0659010:intron 25.0%
! AACTTGTACAAATAGAAAAG+TGG - chr4_3:69258239-69258258 None:intergenic 25.0%
! ATAAAAAATCCAAATCCTGT+TGG + chr4_3:69259057-69259076 Msa0659010:intron 25.0%
!!! TGACCAATATTGTTATTTCT+TGG + chr4_3:69259080-69259099 Msa0659010:intron 25.0%
! TTAGTTGTTCAAATTGTGTT+TGG + chr4_3:69259236-69259255 Msa0659010:three_prime_UTR 25.0%
! AATTCTATTATCCGTAAGTA+TGG + chr4_3:69259279-69259298 Msa0659010:three_prime_UTR 25.0%
TGAACTAGAAATATCCATGT+TGG - chr4_3:69257150-69257169 None:intergenic 30.0%
GACATGAAAACATATGAAGA+AGG - chr4_3:69257237-69257256 None:intergenic 30.0%
!! TGATATTTTGTTTCAGGGTT+GGG + chr4_3:69257319-69257338 Msa0659010:intron 30.0%
! TTGGCAATCTTTTTACTTGT+GGG + chr4_3:69257544-69257563 Msa0659010:intron 30.0%
!!! GTCCAATAGATCCTTTTTTT+AGG + chr4_3:69257605-69257624 Msa0659010:intron 30.0%
AACAGTATAAAGCCACAATT+GGG + chr4_3:69257861-69257880 Msa0659010:CDS 30.0%
! TTTAAAATTACTCACCTGCA+AGG - chr4_3:69257933-69257952 None:intergenic 30.0%
TTACAATGATATGGCAGATT+TGG + chr4_3:69258031-69258050 Msa0659010:intron 30.0%
TACAATGATATGGCAGATTT+GGG + chr4_3:69258032-69258051 Msa0659010:intron 30.0%
AAGATTGTAAGCCAAACAAA+GGG - chr4_3:69258269-69258288 None:intergenic 30.0%
TAAGATTGTAAGCCAAACAA+AGG - chr4_3:69258270-69258289 None:intergenic 30.0%
AATCATTGGATCAAATGGAA+CGG + chr4_3:69258345-69258364 Msa0659010:intron 30.0%
TTTATCTTCCGATAGAATCT+TGG + chr4_3:69258515-69258534 Msa0659010:intron 30.0%
!! CATGGTTCAATTGATTTGAT+CGG - chr4_3:69258546-69258565 None:intergenic 30.0%
TTTGATGTGAAATTGATCTC+AGG + chr4_3:69258635-69258654 Msa0659010:intron 30.0%
CATTTCCCTTTGTATTACTT+GGG + chr4_3:69258679-69258698 Msa0659010:CDS 30.0%
TTGTTCCCAAGTAATACAAA+GGG - chr4_3:69258687-69258706 None:intergenic 30.0%
GCAACAATTAAAAAGGCAAA+TGG - chr4_3:69258824-69258843 None:intergenic 30.0%
!! TTTGCCTTTTTAATTGTTGC+AGG + chr4_3:69258824-69258843 Msa0659010:intron 30.0%
GTAACCTGCAACAATTAAAA+AGG - chr4_3:69258831-69258850 None:intergenic 30.0%
TTTGCAGAGGTTTCAAAATA+TGG - chr4_3:69258897-69258916 None:intergenic 30.0%
CTGCCAAGAAATAACAATAT+TGG - chr4_3:69259086-69259105 None:intergenic 30.0%
!!! AGGAACATAGTTTTGTTCTT+TGG + chr4_3:69259203-69259222 Msa0659010:three_prime_UTR 30.0%
AGAAGGATTATCCATACTTA+CGG - chr4_3:69259293-69259312 None:intergenic 30.0%
!! ATCCTTCTCTGTTTTTCTTT+AGG + chr4_3:69259305-69259324 Msa0659010:three_prime_UTR 30.0%
TGAACTAGAAATATCCATGT+TGG - chr4_3:69257150-69257169 None:intergenic 30.0%
GACATGAAAACATATGAAGA+AGG - chr4_3:69257237-69257256 None:intergenic 30.0%
!! TGATATTTTGTTTCAGGGTT+GGG + chr4_3:69257319-69257338 Msa0659010:intron 30.0%
! TTGGCAATCTTTTTACTTGT+GGG + chr4_3:69257544-69257563 Msa0659010:intron 30.0%
!!! GTCCAATAGATCCTTTTTTT+AGG + chr4_3:69257605-69257624 Msa0659010:intron 30.0%
AACAGTATAAAGCCACAATT+GGG + chr4_3:69257861-69257880 Msa0659010:CDS 30.0%
! TTTAAAATTACTCACCTGCA+AGG - chr4_3:69257933-69257952 None:intergenic 30.0%
TTACAATGATATGGCAGATT+TGG + chr4_3:69258031-69258050 Msa0659010:intron 30.0%
TACAATGATATGGCAGATTT+GGG + chr4_3:69258032-69258051 Msa0659010:intron 30.0%
AAGATTGTAAGCCAAACAAA+GGG - chr4_3:69258269-69258288 None:intergenic 30.0%
TAAGATTGTAAGCCAAACAA+AGG - chr4_3:69258270-69258289 None:intergenic 30.0%
AATCATTGGATCAAATGGAA+CGG + chr4_3:69258345-69258364 Msa0659010:intron 30.0%
TTTATCTTCCGATAGAATCT+TGG + chr4_3:69258515-69258534 Msa0659010:intron 30.0%
!! CATGGTTCAATTGATTTGAT+CGG - chr4_3:69258546-69258565 None:intergenic 30.0%
TTTGATGTGAAATTGATCTC+AGG + chr4_3:69258635-69258654 Msa0659010:intron 30.0%
CATTTCCCTTTGTATTACTT+GGG + chr4_3:69258679-69258698 Msa0659010:CDS 30.0%
TTGTTCCCAAGTAATACAAA+GGG - chr4_3:69258687-69258706 None:intergenic 30.0%
GCAACAATTAAAAAGGCAAA+TGG - chr4_3:69258824-69258843 None:intergenic 30.0%
!! TTTGCCTTTTTAATTGTTGC+AGG + chr4_3:69258824-69258843 Msa0659010:intron 30.0%
GTAACCTGCAACAATTAAAA+AGG - chr4_3:69258831-69258850 None:intergenic 30.0%
TTTGCAGAGGTTTCAAAATA+TGG - chr4_3:69258897-69258916 None:intergenic 30.0%
CTGCCAAGAAATAACAATAT+TGG - chr4_3:69259086-69259105 None:intergenic 30.0%
!!! AGGAACATAGTTTTGTTCTT+TGG + chr4_3:69259203-69259222 Msa0659010:three_prime_UTR 30.0%
AGAAGGATTATCCATACTTA+CGG - chr4_3:69259293-69259312 None:intergenic 30.0%
!! ATCCTTCTCTGTTTTTCTTT+AGG + chr4_3:69259305-69259324 Msa0659010:three_prime_UTR 30.0%
!!! TGAGTTTTGAATCTCCAACA+TGG + chr4_3:69257133-69257152 Msa0659010:exon 35.0%
CAGAAAGAGAACTTTGCTTA+AGG + chr4_3:69257172-69257191 Msa0659010:CDS 35.0%
!! CTGATATTTTGTTTCAGGGT+TGG + chr4_3:69257318-69257337 Msa0659010:intron 35.0%
TTGATGAACCAGTATCCATT+TGG + chr4_3:69257351-69257370 Msa0659010:intron 35.0%
TGAAAATGCCAAATGGATAC+TGG - chr4_3:69257362-69257381 None:intergenic 35.0%
! GTTGGCAATCTTTTTACTTG+TGG + chr4_3:69257543-69257562 Msa0659010:intron 35.0%
! TGGCAATCTTTTTACTTGTG+GGG + chr4_3:69257545-69257564 Msa0659010:intron 35.0%
!! CAATTGTGGCTTTATACTGT+TGG - chr4_3:69257862-69257881 None:intergenic 35.0%
CAACAGTATAAAGCCACAAT+TGG + chr4_3:69257860-69257879 Msa0659010:CDS 35.0%
ACAGTATAAAGCCACAATTG+GGG + chr4_3:69257862-69257881 Msa0659010:CDS 35.0%
!!! AGTTGTTTTTGCCCTTTGTT+TGG + chr4_3:69258255-69258274 Msa0659010:intron 35.0%
ATCAAATGGAACGGCTTAAA+CGG + chr4_3:69258354-69258373 Msa0659010:intron 35.0%
ATCCAAGTTACATTGCAGTT+GGG + chr4_3:69258402-69258421 Msa0659010:intron 35.0%
TTGCATACTCAAAATCGGAT+CGG - chr4_3:69258583-69258602 None:intergenic 35.0%
GCATTTCCCTTTGTATTACT+TGG + chr4_3:69258678-69258697 Msa0659010:CDS 35.0%
CTTGTTCCCAAGTAATACAA+AGG - chr4_3:69258688-69258707 None:intergenic 35.0%
CTTTGTATTACTTGGGAACA+AGG + chr4_3:69258686-69258705 Msa0659010:CDS 35.0%
!! AACAAGGTTGATATAGATGG+TGG + chr4_3:69258702-69258721 Msa0659010:CDS 35.0%
GTGAACACTTTGCATGAAAA+AGG - chr4_3:69258802-69258821 None:intergenic 35.0%
!!! TTTTGAAACCTCTGCAAAAG+AGG + chr4_3:69258899-69258918 Msa0659010:CDS 35.0%
! TTTGAAACCTCTGCAAAAGA+GGG + chr4_3:69258900-69258919 Msa0659010:CDS 35.0%
TAACAATATTGGTCACCAAC+AGG - chr4_3:69259075-69259094 None:intergenic 35.0%
! GTTCAAATTGTGTTTGGTTC+AGG + chr4_3:69259242-69259261 Msa0659010:three_prime_UTR 35.0%
CACCTAAAGAAAAACAGAGA+AGG - chr4_3:69259310-69259329 None:intergenic 35.0%
!!! TGAGTTTTGAATCTCCAACA+TGG + chr4_3:69257133-69257152 Msa0659010:exon 35.0%
CAGAAAGAGAACTTTGCTTA+AGG + chr4_3:69257172-69257191 Msa0659010:CDS 35.0%
!! CTGATATTTTGTTTCAGGGT+TGG + chr4_3:69257318-69257337 Msa0659010:intron 35.0%
TTGATGAACCAGTATCCATT+TGG + chr4_3:69257351-69257370 Msa0659010:intron 35.0%
TGAAAATGCCAAATGGATAC+TGG - chr4_3:69257362-69257381 None:intergenic 35.0%
! GTTGGCAATCTTTTTACTTG+TGG + chr4_3:69257543-69257562 Msa0659010:intron 35.0%
! TGGCAATCTTTTTACTTGTG+GGG + chr4_3:69257545-69257564 Msa0659010:intron 35.0%
!! CAATTGTGGCTTTATACTGT+TGG - chr4_3:69257862-69257881 None:intergenic 35.0%
CAACAGTATAAAGCCACAAT+TGG + chr4_3:69257860-69257879 Msa0659010:CDS 35.0%
ACAGTATAAAGCCACAATTG+GGG + chr4_3:69257862-69257881 Msa0659010:CDS 35.0%
!!! AGTTGTTTTTGCCCTTTGTT+TGG + chr4_3:69258255-69258274 Msa0659010:intron 35.0%
ATCAAATGGAACGGCTTAAA+CGG + chr4_3:69258354-69258373 Msa0659010:intron 35.0%
ATCCAAGTTACATTGCAGTT+GGG + chr4_3:69258402-69258421 Msa0659010:intron 35.0%
TTGCATACTCAAAATCGGAT+CGG - chr4_3:69258583-69258602 None:intergenic 35.0%
GCATTTCCCTTTGTATTACT+TGG + chr4_3:69258678-69258697 Msa0659010:CDS 35.0%
CTTGTTCCCAAGTAATACAA+AGG - chr4_3:69258688-69258707 None:intergenic 35.0%
CTTTGTATTACTTGGGAACA+AGG + chr4_3:69258686-69258705 Msa0659010:CDS 35.0%
!! AACAAGGTTGATATAGATGG+TGG + chr4_3:69258702-69258721 Msa0659010:CDS 35.0%
GTGAACACTTTGCATGAAAA+AGG - chr4_3:69258802-69258821 None:intergenic 35.0%
!!! TTTTGAAACCTCTGCAAAAG+AGG + chr4_3:69258899-69258918 Msa0659010:CDS 35.0%
! TTTGAAACCTCTGCAAAAGA+GGG + chr4_3:69258900-69258919 Msa0659010:CDS 35.0%
TAACAATATTGGTCACCAAC+AGG - chr4_3:69259075-69259094 None:intergenic 35.0%
! GTTCAAATTGTGTTTGGTTC+AGG + chr4_3:69259242-69259261 Msa0659010:three_prime_UTR 35.0%
CACCTAAAGAAAAACAGAGA+AGG - chr4_3:69259310-69259329 None:intergenic 35.0%
TTGCTTAAGGTCATTGTCCT+TGG + chr4_3:69257185-69257204 Msa0659010:CDS 40.0%
TCATTGTCCTTGGAGATAGT+GGG + chr4_3:69257195-69257214 Msa0659010:CDS 40.0%
TAACATACCCACTATCTCCA+AGG - chr4_3:69257205-69257224 None:intergenic 40.0%
!! ACCAAATTTGGTGCATGAGT+TGG + chr4_3:69257525-69257544 Msa0659010:intron 40.0%
! TTTTTACTTGTGGGGAACAG+TGG + chr4_3:69257553-69257572 Msa0659010:intron 40.0%
! ATCCATCATAATTTGCCAGC+TGG - chr4_3:69257585-69257604 None:intergenic 40.0%
AACAAAATCAGCCCCAATTG+TGG - chr4_3:69257876-69257895 None:intergenic 40.0%
!! TGGGGCTGATTTTGTTACAA+AGG + chr4_3:69257880-69257899 Msa0659010:CDS 40.0%
TGATAAACTCGTAACCTTGC+AGG + chr4_3:69257916-69257935 Msa0659010:CDS 40.0%
AAAATGCAGCTCCAAGACTA+TGG - chr4_3:69258080-69258099 None:intergenic 40.0%
! TTGGAGCTGCATTTTACAGA+GGG + chr4_3:69258085-69258104 Msa0659010:CDS 40.0%
GCATGATGACTTTCTTAAGC+AGG + chr4_3:69258173-69258192 Msa0659010:CDS 40.0%
GATCCAAGTTACATTGCAGT+TGG + chr4_3:69258401-69258420 Msa0659010:intron 40.0%
AACCCAACTGCAATGTAACT+TGG - chr4_3:69258407-69258426 None:intergenic 40.0%
CGGTTCAACCAAGATTCTAT+CGG - chr4_3:69258526-69258545 None:intergenic 40.0%
AATTGATCTCAGGCAGATAC+AGG + chr4_3:69258645-69258664 Msa0659010:intron 40.0%
AATACAAAGGGAAATGCCTC+AGG - chr4_3:69258675-69258694 None:intergenic 40.0%
!! GGGAACAAGGTTGATATAGA+TGG + chr4_3:69258699-69258718 Msa0659010:CDS 40.0%
!! TAGATGGTGGAAACAGTAGA+AGG + chr4_3:69258715-69258734 Msa0659010:CDS 40.0%
!! AGATGGTGGAAACAGTAGAA+GGG + chr4_3:69258716-69258735 Msa0659010:CDS 40.0%
AGGTTACAGAGAAGAAAGCT+AGG + chr4_3:69258844-69258863 Msa0659010:intron 40.0%
GGTTACAGAGAAGAAAGCTA+GGG + chr4_3:69258845-69258864 Msa0659010:intron 40.0%
TATTGGTCACCAACAGGATT+TGG - chr4_3:69259069-69259088 None:intergenic 40.0%
! TTTCTTGGCAGTTATTTCCG+TGG + chr4_3:69259095-69259114 Msa0659010:intron 40.0%
AAGGGGTTTCAGATATTCCA+CGG - chr4_3:69259115-69259134 None:intergenic 40.0%
TTCGTTGTTCTGCTTCTGAA+GGG - chr4_3:69259133-69259152 None:intergenic 40.0%
! CATGCTGAAGTTGTCGTTTA+AGG + chr4_3:69259162-69259181 Msa0659010:exon 40.0%
! TGTGTTTGGTTCAGGTTCAA+TGG + chr4_3:69259250-69259269 Msa0659010:three_prime_UTR 40.0%
! GTGTTTGGTTCAGGTTCAAT+GGG + chr4_3:69259251-69259270 Msa0659010:three_prime_UTR 40.0%
TTGCTTAAGGTCATTGTCCT+TGG + chr4_3:69257185-69257204 Msa0659010:CDS 40.0%
TCATTGTCCTTGGAGATAGT+GGG + chr4_3:69257195-69257214 Msa0659010:CDS 40.0%
TAACATACCCACTATCTCCA+AGG - chr4_3:69257205-69257224 None:intergenic 40.0%
!! ACCAAATTTGGTGCATGAGT+TGG + chr4_3:69257525-69257544 Msa0659010:intron 40.0%
! TTTTTACTTGTGGGGAACAG+TGG + chr4_3:69257553-69257572 Msa0659010:intron 40.0%
! ATCCATCATAATTTGCCAGC+TGG - chr4_3:69257585-69257604 None:intergenic 40.0%
AACAAAATCAGCCCCAATTG+TGG - chr4_3:69257876-69257895 None:intergenic 40.0%
!! TGGGGCTGATTTTGTTACAA+AGG + chr4_3:69257880-69257899 Msa0659010:CDS 40.0%
TGATAAACTCGTAACCTTGC+AGG + chr4_3:69257916-69257935 Msa0659010:CDS 40.0%
AAAATGCAGCTCCAAGACTA+TGG - chr4_3:69258080-69258099 None:intergenic 40.0%
! TTGGAGCTGCATTTTACAGA+GGG + chr4_3:69258085-69258104 Msa0659010:CDS 40.0%
GCATGATGACTTTCTTAAGC+AGG + chr4_3:69258173-69258192 Msa0659010:CDS 40.0%
GATCCAAGTTACATTGCAGT+TGG + chr4_3:69258401-69258420 Msa0659010:intron 40.0%
AACCCAACTGCAATGTAACT+TGG - chr4_3:69258407-69258426 None:intergenic 40.0%
CGGTTCAACCAAGATTCTAT+CGG - chr4_3:69258526-69258545 None:intergenic 40.0%
AATTGATCTCAGGCAGATAC+AGG + chr4_3:69258645-69258664 Msa0659010:intron 40.0%
AATACAAAGGGAAATGCCTC+AGG - chr4_3:69258675-69258694 None:intergenic 40.0%
!! GGGAACAAGGTTGATATAGA+TGG + chr4_3:69258699-69258718 Msa0659010:CDS 40.0%
!! TAGATGGTGGAAACAGTAGA+AGG + chr4_3:69258715-69258734 Msa0659010:CDS 40.0%
!! AGATGGTGGAAACAGTAGAA+GGG + chr4_3:69258716-69258735 Msa0659010:CDS 40.0%
AGGTTACAGAGAAGAAAGCT+AGG + chr4_3:69258844-69258863 Msa0659010:intron 40.0%
GGTTACAGAGAAGAAAGCTA+GGG + chr4_3:69258845-69258864 Msa0659010:intron 40.0%
TATTGGTCACCAACAGGATT+TGG - chr4_3:69259069-69259088 None:intergenic 40.0%
! TTTCTTGGCAGTTATTTCCG+TGG + chr4_3:69259095-69259114 Msa0659010:intron 40.0%
AAGGGGTTTCAGATATTCCA+CGG - chr4_3:69259115-69259134 None:intergenic 40.0%
TTCGTTGTTCTGCTTCTGAA+GGG - chr4_3:69259133-69259152 None:intergenic 40.0%
! CATGCTGAAGTTGTCGTTTA+AGG + chr4_3:69259162-69259181 Msa0659010:exon 40.0%
! TGTGTTTGGTTCAGGTTCAA+TGG + chr4_3:69259250-69259269 Msa0659010:three_prime_UTR 40.0%
! GTGTTTGGTTCAGGTTCAAT+GGG + chr4_3:69259251-69259270 Msa0659010:three_prime_UTR 40.0%
GTCATTGTCCTTGGAGATAG+TGG + chr4_3:69257194-69257213 Msa0659010:CDS 45.0%
GCCAACTCATGCACCAAATT+TGG - chr4_3:69257529-69257548 None:intergenic 45.0%
GTCCAGCTGGCAAATTATGA+TGG + chr4_3:69257580-69257599 Msa0659010:intron 45.0%
CAGGAAAGGTTCCATAGTCT+TGG + chr4_3:69258066-69258085 Msa0659010:CDS 45.0%
! CTTGGAGCTGCATTTTACAG+AGG + chr4_3:69258084-69258103 Msa0659010:CDS 45.0%
! TGGAGCTGCATTTTACAGAG+GGG + chr4_3:69258086-69258105 Msa0659010:CDS 45.0%
! AGGGGCAGATTGTTGTGTTT+TGG + chr4_3:69258104-69258123 Msa0659010:CDS 45.0%
AATCGGATCGGTGAGACTTT+CGG - chr4_3:69258571-69258590 None:intergenic 45.0%
CAGAGAAGAAAGCTAGGGAA+TGG + chr4_3:69258850-69258869 Msa0659010:CDS 45.0%
TAGGGAATGGTGTGCTTCTA+GGG + chr4_3:69258863-69258882 Msa0659010:CDS 45.0%
! ATTGTAGCCCTCTTTTGCAG+AGG - chr4_3:69258910-69258929 None:intergenic 45.0%
! TCGTTGTTCTGCTTCTGAAG+GGG - chr4_3:69259132-69259151 None:intergenic 45.0%
CTTCGTTGTTCTGCTTCTGA+AGG - chr4_3:69259134-69259153 None:intergenic 45.0%
GTCATTGTCCTTGGAGATAG+TGG + chr4_3:69257194-69257213 Msa0659010:CDS 45.0%
GCCAACTCATGCACCAAATT+TGG - chr4_3:69257529-69257548 None:intergenic 45.0%
GTCCAGCTGGCAAATTATGA+TGG + chr4_3:69257580-69257599 Msa0659010:intron 45.0%
CAGGAAAGGTTCCATAGTCT+TGG + chr4_3:69258066-69258085 Msa0659010:CDS 45.0%
! CTTGGAGCTGCATTTTACAG+AGG + chr4_3:69258084-69258103 Msa0659010:CDS 45.0%
! TGGAGCTGCATTTTACAGAG+GGG + chr4_3:69258086-69258105 Msa0659010:CDS 45.0%
! AGGGGCAGATTGTTGTGTTT+TGG + chr4_3:69258104-69258123 Msa0659010:CDS 45.0%
AATCGGATCGGTGAGACTTT+CGG - chr4_3:69258571-69258590 None:intergenic 45.0%
CAGAGAAGAAAGCTAGGGAA+TGG + chr4_3:69258850-69258869 Msa0659010:CDS 45.0%
TAGGGAATGGTGTGCTTCTA+GGG + chr4_3:69258863-69258882 Msa0659010:CDS 45.0%
! ATTGTAGCCCTCTTTTGCAG+AGG - chr4_3:69258910-69258929 None:intergenic 45.0%
! TCGTTGTTCTGCTTCTGAAG+GGG - chr4_3:69259132-69259151 None:intergenic 45.0%
CTTCGTTGTTCTGCTTCTGA+AGG - chr4_3:69259134-69259153 None:intergenic 45.0%
!!! AAATTTTTAACATAAAATTA+TGG + chr4_3:69257754-69257773 Msa0659010:intron 5.0%
!! AATAATTGTAAAAAATAAAA+AGG - chr4_3:69258757-69258776 None:intergenic 5.0%
!!! AAATTTTTAACATAAAATTA+TGG + chr4_3:69257754-69257773 Msa0659010:intron 5.0%
!! AATAATTGTAAAAAATAAAA+AGG - chr4_3:69258757-69258776 None:intergenic 5.0%
! TGGCAGATTTGGGATACTGC+AGG + chr4_3:69258042-69258061 Msa0659010:intron 50.0%
! GATTTGGGATACTGCAGGAC+AGG + chr4_3:69258047-69258066 Msa0659010:intron 50.0%
ATCGGATCGGTGAGACTTTC+GGG - chr4_3:69258570-69258589 None:intergenic 50.0%
CTAGGGAATGGTGTGCTTCT+AGG + chr4_3:69258862-69258881 Msa0659010:CDS 50.0%
AGGGAATGGTGTGCTTCTAG+GGG + chr4_3:69258864-69258883 Msa0659010:CDS 50.0%
! TGGCAGATTTGGGATACTGC+AGG + chr4_3:69258042-69258061 Msa0659010:intron 50.0%
! GATTTGGGATACTGCAGGAC+AGG + chr4_3:69258047-69258066 Msa0659010:intron 50.0%
ATCGGATCGGTGAGACTTTC+GGG - chr4_3:69258570-69258589 None:intergenic 50.0%
CTAGGGAATGGTGTGCTTCT+AGG + chr4_3:69258862-69258881 Msa0659010:CDS 50.0%
AGGGAATGGTGTGCTTCTAG+GGG + chr4_3:69258864-69258883 Msa0659010:CDS 50.0%
GAACAGTGGTTCAGTCCAGC+TGG + chr4_3:69257567-69257586 Msa0659010:intron 55.0%
GGGATACTGCAGGACAGGAA+AGG + chr4_3:69258052-69258071 Msa0659010:CDS 55.0%
! TCGGTGAGACTTTCGGGTCA+TGG - chr4_3:69258564-69258583 None:intergenic 55.0%
GGCAGATACAGGCAATCCTG+AGG + chr4_3:69258656-69258675 Msa0659010:intron 55.0%
GAACAGTGGTTCAGTCCAGC+TGG + chr4_3:69257567-69257586 Msa0659010:intron 55.0%
GGGATACTGCAGGACAGGAA+AGG + chr4_3:69258052-69258071 Msa0659010:CDS 55.0%
! TCGGTGAGACTTTCGGGTCA+TGG - chr4_3:69258564-69258583 None:intergenic 55.0%
GGCAGATACAGGCAATCCTG+AGG + chr4_3:69258656-69258675 Msa0659010:intron 55.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 69257111 69259439 69257111 ID=Msa0659010;Name=Msa0659010
chr4_3 mRNA 69257111 69259439 69257111 ID=Msa0659010-mRNA-1;Parent=Msa0659010;Name=Msa0659010-mRNA-1;_AED=0.14;_eAED=0.14;_QI=41|1|1|1|1|1|7|270|209
chr4_3 exon 69257111 69257216 69257111 ID=Msa0659010-mRNA-1:exon:20258;Parent=Msa0659010-mRNA-1
chr4_3 exon 69257335 69257361 69257335 ID=Msa0659010-mRNA-1:exon:20259;Parent=Msa0659010-mRNA-1
chr4_3 exon 69257838 69257937 69257838 ID=Msa0659010-mRNA-1:exon:20260;Parent=Msa0659010-mRNA-1
chr4_3 exon 69258048 69258194 69258048 ID=Msa0659010-mRNA-1:exon:20261;Parent=Msa0659010-mRNA-1
chr4_3 exon 69258657 69258737 69258657 ID=Msa0659010-mRNA-1:exon:20262;Parent=Msa0659010-mRNA-1
chr4_3 exon 69258846 69258991 69258846 ID=Msa0659010-mRNA-1:exon:20263;Parent=Msa0659010-mRNA-1
chr4_3 exon 69259106 69259439 69259106 ID=Msa0659010-mRNA-1:exon:20264;Parent=Msa0659010-mRNA-1
chr4_3 five_prime_UTR 69257111 69257151 69257111 ID=Msa0659010-mRNA-1:five_prime_utr;Parent=Msa0659010-mRNA-1
chr4_3 CDS 69257152 69257216 69257152 ID=Msa0659010-mRNA-1:cds;Parent=Msa0659010-mRNA-1
chr4_3 CDS 69257335 69257361 69257335 ID=Msa0659010-mRNA-1:cds;Parent=Msa0659010-mRNA-1
chr4_3 CDS 69257838 69257937 69257838 ID=Msa0659010-mRNA-1:cds;Parent=Msa0659010-mRNA-1
chr4_3 CDS 69258048 69258194 69258048 ID=Msa0659010-mRNA-1:cds;Parent=Msa0659010-mRNA-1
chr4_3 CDS 69258657 69258737 69258657 ID=Msa0659010-mRNA-1:cds;Parent=Msa0659010-mRNA-1
chr4_3 CDS 69258846 69258991 69258846 ID=Msa0659010-mRNA-1:cds;Parent=Msa0659010-mRNA-1
chr4_3 CDS 69259106 69259169 69259106 ID=Msa0659010-mRNA-1:cds;Parent=Msa0659010-mRNA-1
chr4_3 three_prime_UTR 69259170 69259439 69259170 ID=Msa0659010-mRNA-1:three_prime_utr;Parent=Msa0659010-mRNA-1
Gene Sequence

>Msa0659010

ATGGATATTTCTAGTTCACACAGAAAGAGAACTTTGCTTAAGGTCATTGTCCTTGGAGATAGTGGGGTTGGGAAGACATCTTTGATGAACCAATATGTGTATAAAAAATTTAGCCAACAGTATAAAGCCACAATTGGGGCTGATTTTGTTACAAAGGAGCTGCTAGTTGATGATAAACTCGTAACCTTGCAGATTTGGGATACTGCAGGACAGGAAAGGTTCCATAGTCTTGGAGCTGCATTTTACAGAGGGGCAGATTGTTGTGTTTTGGTATATGATGTAAATATACACAAAACATTTGATACATTAAACAATTGGCATGATGACTTTCTTAAGCAGGCAGATACAGGCAATCCTGAGGCATTTCCCTTTGTATTACTTGGGAACAAGGTTGATATAGATGGTGGAAACAGTAGAAGGGTTACAGAGAAGAAAGCTAGGGAATGGTGTGCTTCTAGGGGTAACATACCATATTTTGAAACCTCTGCAAAAGAGGGCTACAATGTTGATGATGCATTTTTATGTGTTGCTAAAGTTGCATCAGAAAATGAACACGATTTAGACATTTATTTCCGTGGAATATCTGAAACCCCTTCAGAAGCAGAACAACGAAGTAATTGTGCATGCTGA
Protein sequence

>Msa0659010

MDISSSHRKRTLLKVIVLGDSGVGKTSLMNQYVYKKFSQQYKATIGADFVTKELLVDDKLVTLQIWDTAGQERFHSLGAAFYRGADCCVLVYDVNIHKTFDTLNNWHDDFLKQADTGNPEAFPFVLLGNKVDIDGGNSRRVTEKKAREWCASRGNIPYFETSAKEGYNVDDAFLCVAKVASENEHDLDIYFRGISETPSEAEQRSNCAC*