AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene42682


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene42682.t1 MTR_3g104980 95.261 211 10 0 1 211 1 211 3.08e-143 398
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene42682.t1 AT5G21430 55.738 183 77 3 32 210 35 217 1.83e-62 194
MS.gene42682.t1 AT5G21430 55.191 183 77 4 32 210 35 216 5.39e-60 187

Find 50 sgRNAs with CRISPR-Local

Find 74 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTAAAGAAGATGATTCTTT+TGG 0.261740 3.3:-86356029 None:intergenic
AGACATACTTAACAAGAAAT+TGG 0.280104 3.3:+86356461 MS.gene42682:CDS
ATTGGAGAGTCAAGGTTTAG+AGG 0.312619 3.3:+86356437 MS.gene42682:CDS
GCCATTTGAGGTTGATGTTT+CGG 0.315689 3.3:+86356680 MS.gene42682:CDS
TAATAACAAGGTTCGAGAAT+TGG 0.322385 3.3:+86356419 MS.gene42682:CDS
CTAGCATTTGTGTTATCAAT+TGG 0.334884 3.3:+86356919 MS.gene42682:CDS
ATTACAGATGCAGATCATTA+TGG 0.366825 3.3:+86356179 MS.gene42682:CDS
TGTGTCTGTGTTATCTTCTC+TGG 0.408544 3.3:-86356649 None:intergenic
ACCTTGTTATTATAAGCAAT+TGG 0.411448 3.3:-86356408 None:intergenic
CACATACGTTTGGCCATTTG+AGG 0.421335 3.3:+86356668 MS.gene42682:CDS
TCATACATTCTTCTTTCATC+CGG 0.438615 3.3:-86356615 None:intergenic
ATGTTTCTGACCATGTCATA+TGG 0.442154 3.3:-86356227 None:intergenic
TTGCTTGGAGTGTTGTTACT+TGG 0.444787 3.3:-86355882 None:intergenic
GTTGAGAATCCTAGCTTGCT+TGG 0.458033 3.3:-86355897 None:intergenic
CTGGTTGGTCCTACATCCTC+AGG 0.458680 3.3:-86356862 None:intergenic
ACCGAAACATCAACCTCAAA+TGG 0.480733 3.3:-86356681 None:intergenic
GCAGATCATTATGGTAGACT+TGG 0.523390 3.3:+86356188 MS.gene42682:CDS
GCAAATTGTAGTGGTGGTGG+TGG 0.527140 3.3:-86355856 None:intergenic
AGCTCTAGTGAAAACTCTGC+AGG 0.533335 3.3:+86355969 MS.gene42682:CDS
TAGAAAGTATCCAACCAGTC+TGG 0.536223 3.3:-86356881 None:intergenic
CTGGTTGGATACTTTCTAGC+GGG 0.536918 3.3:+86356886 MS.gene42682:CDS
ATAACACAGACACATACGTT+TGG 0.539673 3.3:+86356658 MS.gene42682:CDS
ATGAAAGAAGAATGTATGAC+TGG 0.543817 3.3:+86356619 MS.gene42682:CDS
GATGTTTCGGTACTTCAAGA+AGG 0.552371 3.3:+86356693 MS.gene42682:CDS
ATGTCATATGGACATCCTCT+TGG 0.556234 3.3:-86356215 None:intergenic
AGTGGTGGTGGTGGAGGACA+AGG 0.559487 3.3:-86355847 None:intergenic
AATTGTAGTGGTGGTGGTGG+AGG 0.559668 3.3:-86355853 None:intergenic
TTGGATACTTTCTAGCGGGA+TGG 0.565729 3.3:+86356890 MS.gene42682:CDS
GTAATTAGTACCTGTGGTGG+AGG 0.566183 3.3:-86356720 None:intergenic
GTTCGAGAATTGGAGAGTCA+AGG 0.571210 3.3:+86356429 MS.gene42682:CDS
GTAGGACCAACCAGACTGGT+TGG 0.579118 3.3:+86356871 MS.gene42682:CDS
TGAAAACTCTGCAGGTTCAA+CGG 0.580415 3.3:+86355977 MS.gene42682:CDS
CAAGTAATTAGTACCTGTGG+TGG 0.590198 3.3:-86356723 None:intergenic
GGATGTAGGACCAACCAGAC+TGG 0.590754 3.3:+86356867 MS.gene42682:CDS
TGGCAGGAACCTGAGGATGT+AGG 0.592315 3.3:+86356853 MS.gene42682:intron
AACAACACTCCAAGCAAGCT+AGG 0.601547 3.3:+86355888 MS.gene42682:CDS
ATACACTATCATGTCATCGC+CGG 0.604771 3.3:+86356596 MS.gene42682:CDS
GCCAATTGCTTATAATAACA+AGG 0.606196 3.3:+86356407 MS.gene42682:CDS
AATGCAAATTGTAGTGGTGG+TGG 0.612182 3.3:-86355859 None:intergenic
AGGTTCAACGGAGGTTGAGT+CGG 0.620899 3.3:+86355989 MS.gene42682:CDS
AAGTATCCAACCAGTCTGGT+TGG 0.621927 3.3:-86356877 None:intergenic
AGAAGGAGATCCTCCACCAC+AGG 0.633320 3.3:+86356710 MS.gene42682:CDS
ACTTGGAATGCAAATTGTAG+TGG 0.640752 3.3:-86355865 None:intergenic
GGTAGACTTGGCTTACCAAG+AGG 0.643717 3.3:+86356200 MS.gene42682:CDS
TGGAAAACATACGTATGCCA+CGG 0.651444 3.3:-86356079 None:intergenic
TGGAATGCAAATTGTAGTGG+TGG 0.664067 3.3:-86355862 None:intergenic
AAGAGGATGTCCATATGACA+TGG 0.675165 3.3:+86356217 MS.gene42682:CDS
ACTGGTTGGATACTTTCTAG+CGG 0.691428 3.3:+86356885 MS.gene42682:CDS
AACGTCGAAAGAGCTCTCCG+TGG 0.728158 3.3:+86356062 MS.gene42682:CDS
AAACTCTGCAGGTTCAACGG+AGG 0.732304 3.3:+86355980 MS.gene42682:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATAATAACAACAACATTA+TGG - chr3.3:86356102-86356121 None:intergenic 15.0%
!! TTGAAATGATCCATTTAAAA+CGG + chr3.3:86356815-86356834 MS.gene42682:intron 20.0%
!!! ATTAAAGAAGATGATTCTTT+TGG - chr3.3:86356032-86356051 None:intergenic 20.0%
! AGACATACTTAACAAGAAAT+TGG + chr3.3:86356461-86356480 MS.gene42682:CDS 25.0%
! GAATTGAATTGATGAAGATA+TGG + chr3.3:86356142-86356161 MS.gene42682:intron 25.0%
! GTATGATTCCTATACAATTA+AGG - chr3.3:86356580-86356599 None:intergenic 25.0%
! TCGGATTTAATTCATATGAA+AGG + chr3.3:86356256-86356275 MS.gene42682:intron 25.0%
! TGTGTTTGCATCATTATTAT+AGG + chr3.3:86356525-86356544 MS.gene42682:intron 25.0%
! TTGGTTAACCTTAATTGTAT+AGG + chr3.3:86356569-86356588 MS.gene42682:intron 25.0%
!! TGGTCAGAAACATTTTATAT+CGG + chr3.3:86356237-86356256 MS.gene42682:intron 25.0%
!! TTTCTATTTCTCAACTTCTT+AGG + chr3.3:86356746-86356765 MS.gene42682:intron 25.0%
!!! ACCTTGTTATTATAAGCAAT+TGG - chr3.3:86356411-86356430 None:intergenic 25.0%
!!! ATTGGAAATACCGTTTTAAA+TGG - chr3.3:86356828-86356847 None:intergenic 25.0%
AAACAAGTAATTAGTACCTG+TGG - chr3.3:86356729-86356748 None:intergenic 30.0%
ATGAAAGAAGAATGTATGAC+TGG + chr3.3:86356619-86356638 MS.gene42682:CDS 30.0%
ATGTCACTCTATACTTTAAC+AGG + chr3.3:86356383-86356402 MS.gene42682:intron 30.0%
ATTACAGATGCAGATCATTA+TGG + chr3.3:86356179-86356198 MS.gene42682:CDS 30.0%
CTAGCATTTGTGTTATCAAT+TGG + chr3.3:86356919-86356938 MS.gene42682:CDS 30.0%
GCCAATTGCTTATAATAACA+AGG + chr3.3:86356407-86356426 MS.gene42682:CDS 30.0%
TAATAACAAGGTTCGAGAAT+TGG + chr3.3:86356419-86356438 MS.gene42682:CDS 30.0%
TCATACATTCTTCTTTCATC+CGG - chr3.3:86356618-86356637 None:intergenic 30.0%
! AACGGTATTTCCAATTTTCA+TGG + chr3.3:86356833-86356852 MS.gene42682:intron 30.0%
!! TGATTCTTTTGGAAGTTCAA+TGG - chr3.3:86356021-86356040 None:intergenic 30.0%
!!! TTTTTTCTGTAAAACAAGCG+CGG + chr3.3:86355931-86355950 MS.gene42682:CDS 30.0%
ACTTGGAATGCAAATTGTAG+TGG - chr3.3:86355868-86355887 None:intergenic 35.0%
ATAACACAGACACATACGTT+TGG + chr3.3:86356658-86356677 MS.gene42682:CDS 35.0%
TATTATAGGTGAGACTTGAG+AGG + chr3.3:86356539-86356558 MS.gene42682:intron 35.0%
! ATGTTTCTGACCATGTCATA+TGG - chr3.3:86356230-86356249 None:intergenic 35.0%
! CTATTTCTCAACTTCTTAGG+AGG + chr3.3:86356749-86356768 MS.gene42682:intron 35.0%
! GTATTTCCAATTTTCATGGC+AGG + chr3.3:86356837-86356856 MS.gene42682:intron 35.0%
AAGAGGATGTCCATATGACA+TGG + chr3.3:86356217-86356236 MS.gene42682:CDS 40.0%
AATGCAAATTGTAGTGGTGG+TGG - chr3.3:86355862-86355881 None:intergenic 40.0%
ACCGAAACATCAACCTCAAA+TGG - chr3.3:86356684-86356703 None:intergenic 40.0%
ACTGGTTGGATACTTTCTAG+CGG + chr3.3:86356885-86356904 MS.gene42682:CDS 40.0%
ATACACTATCATGTCATCGC+CGG + chr3.3:86356596-86356615 MS.gene42682:CDS 40.0%
ATGTCATATGGACATCCTCT+TGG - chr3.3:86356218-86356237 None:intergenic 40.0%
ATTGGAGAGTCAAGGTTTAG+AGG + chr3.3:86356437-86356456 MS.gene42682:CDS 40.0%
CAAGTAATTAGTACCTGTGG+TGG - chr3.3:86356726-86356745 None:intergenic 40.0%
GATGTTTCGGTACTTCAAGA+AGG + chr3.3:86356693-86356712 MS.gene42682:CDS 40.0%
GCAGATCATTATGGTAGACT+TGG + chr3.3:86356188-86356207 MS.gene42682:CDS 40.0%
TGAAAACTCTGCAGGTTCAA+CGG + chr3.3:86355977-86355996 MS.gene42682:CDS 40.0%
TGGAAAACATACGTATGCCA+CGG - chr3.3:86356082-86356101 None:intergenic 40.0%
TGGAATGCAAATTGTAGTGG+TGG - chr3.3:86355865-86355884 None:intergenic 40.0%
TGTGTCTGTGTTATCTTCTC+TGG - chr3.3:86356652-86356671 None:intergenic 40.0%
!! AACTTCTTAGGAGGTGTTTG+AGG + chr3.3:86356758-86356777 MS.gene42682:intron 40.0%
!! GCCATTTGAGGTTGATGTTT+CGG + chr3.3:86356680-86356699 MS.gene42682:CDS 40.0%
!! TAGAAAGTATCCAACCAGTC+TGG - chr3.3:86356884-86356903 None:intergenic 40.0%
!! TTGCTTGGAGTGTTGTTACT+TGG - chr3.3:86355885-86355904 None:intergenic 40.0%
AACAACACTCCAAGCAAGCT+AGG + chr3.3:86355888-86355907 MS.gene42682:CDS 45.0%
AAGTATCCAACCAGTCTGGT+TGG - chr3.3:86356880-86356899 None:intergenic 45.0%
AGACTTGAGAGGTGGTGATT+TGG + chr3.3:86356550-86356569 MS.gene42682:intron 45.0%
CACATACGTTTGGCCATTTG+AGG + chr3.3:86356668-86356687 MS.gene42682:CDS 45.0%
CAGGTTCCTGCCATGAAAAT+TGG - chr3.3:86356846-86356865 None:intergenic 45.0%
GTAATTAGTACCTGTGGTGG+AGG - chr3.3:86356723-86356742 None:intergenic 45.0%
GTTCGAGAATTGGAGAGTCA+AGG + chr3.3:86356429-86356448 MS.gene42682:CDS 45.0%
TATAGGTGAGACTTGAGAGG+TGG + chr3.3:86356542-86356561 MS.gene42682:intron 45.0%
! AGCTCTAGTGAAAACTCTGC+AGG + chr3.3:86355969-86355988 MS.gene42682:CDS 45.0%
! ATTTTCATGGCAGGAACCTG+AGG + chr3.3:86356846-86356865 MS.gene42682:intron 45.0%
! CTGGTTGGATACTTTCTAGC+GGG + chr3.3:86356886-86356905 MS.gene42682:CDS 45.0%
! TTGGATACTTTCTAGCGGGA+TGG + chr3.3:86356890-86356909 MS.gene42682:CDS 45.0%
!! GTTGAGAATCCTAGCTTGCT+TGG - chr3.3:86355900-86355919 None:intergenic 45.0%
!! TTCTTAGGAGGTGTTTGAGG+TGG + chr3.3:86356761-86356780 MS.gene42682:intron 45.0%
AAACTCTGCAGGTTCAACGG+AGG + chr3.3:86355980-86355999 MS.gene42682:CDS 50.0%
AGGTTCAACGGAGGTTGAGT+CGG + chr3.3:86355989-86356008 MS.gene42682:CDS 50.0%
GGTAGACTTGGCTTACCAAG+AGG + chr3.3:86356200-86356219 MS.gene42682:CDS 50.0%
!! AATTGTAGTGGTGGTGGTGG+AGG - chr3.3:86355856-86355875 None:intergenic 50.0%
!! GCAAATTGTAGTGGTGGTGG+TGG - chr3.3:86355859-86355878 None:intergenic 50.0%
AACGTCGAAAGAGCTCTCCG+TGG + chr3.3:86356062-86356081 MS.gene42682:CDS 55.0%
AGAAGGAGATCCTCCACCAC+AGG + chr3.3:86356710-86356729 MS.gene42682:CDS 55.0%
GGATGTAGGACCAACCAGAC+TGG + chr3.3:86356867-86356886 MS.gene42682:CDS 55.0%
GTAGGACCAACCAGACTGGT+TGG + chr3.3:86356871-86356890 MS.gene42682:CDS 55.0%
TGGCAGGAACCTGAGGATGT+AGG + chr3.3:86356853-86356872 MS.gene42682:intron 55.0%
! CTGGTTGGTCCTACATCCTC+AGG - chr3.3:86356865-86356884 None:intergenic 55.0%
!! AGTGGTGGTGGTGGAGGACA+AGG - chr3.3:86355850-86355869 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 86355843 86356954 86355843 ID=MS.gene42682
chr3.3 mRNA 86355843 86356954 86355843 ID=MS.gene42682.t1;Parent=MS.gene42682
chr3.3 exon 86355843 86356089 86355843 ID=MS.gene42682.t1.exon1;Parent=MS.gene42682.t1
chr3.3 CDS 86355843 86356089 86355843 ID=cds.MS.gene42682.t1;Parent=MS.gene42682.t1
chr3.3 exon 86356177 86356238 86356177 ID=MS.gene42682.t1.exon2;Parent=MS.gene42682.t1
chr3.3 CDS 86356177 86356238 86356177 ID=cds.MS.gene42682.t1;Parent=MS.gene42682.t1
chr3.3 exon 86356405 86356494 86356405 ID=MS.gene42682.t1.exon3;Parent=MS.gene42682.t1
chr3.3 CDS 86356405 86356494 86356405 ID=cds.MS.gene42682.t1;Parent=MS.gene42682.t1
chr3.3 exon 86356591 86356731 86356591 ID=MS.gene42682.t1.exon4;Parent=MS.gene42682.t1
chr3.3 CDS 86356591 86356731 86356591 ID=cds.MS.gene42682.t1;Parent=MS.gene42682.t1
chr3.3 exon 86356859 86356954 86356859 ID=MS.gene42682.t1.exon5;Parent=MS.gene42682.t1
chr3.3 CDS 86356859 86356954 86356859 ID=cds.MS.gene42682.t1;Parent=MS.gene42682.t1
Gene Sequence

>MS.gene42682

ATGGCCTTGTCCTCCACCACCACCACTACAATTTGCATTCCAAGTAACAACACTCCAAGCAAGCTAGGATTCTCAACTAATAACATTGTTTTTTCTGTAAAACAAGCGCGGAAACTATGTATCAGAAGCTCTAGTGAAAACTCTGCAGGTTCAACGGAGGTTGAGTCGGAGAGTTCCATTGAACTTCCAAAAGAATCATCTTCTTTAATTTCTGTTCTCAACGTCGAAAGAGCTCTCCGTGGCATACGTATGTTTTCCATAATGTTGTTGTTATTATATGCTTAAAATTGCATTGCATTGAATTGAATTGATGAAGATATGGTTTAGAATGCAGCTATTACAGATGCAGATCATTATGGTAGACTTGGCTTACCAAGAGGATGTCCATATGACATGGTCAGAAACATTTTATATCGGATTTAATTCATATGAAAGGATTTTTATACAGTCAATCAATCATATTCGTCAAACATGAAAATCATTTGACTTCTAAAGTCACGCATGTATGATTGATGTTAATTCAACTTATCTTTTTGATCTATGTCACTCTATACTTTAACAGGTGCCAATTGCTTATAATAACAAGGTTCGAGAATTGGAGAGTCAAGGTTTAGAGGAAGACATACTTAACAAGAAATTGGAACTTCTCAGAGTTAGTATATATGCATGATTTGAATTGTTGTGTGTTTGCATCATTATTATAGGTGAGACTTGAGAGGTGGTGATTTGGTTAACCTTAATTGTATAGGAATCATACACTATCATGTCATCGCCGGATGAAAGAAGAATGTATGACTGGAGCTTGTCCAGAGAAGATAACACAGACACATACGTTTGGCCATTTGAGGTTGATGTTTCGGTACTTCAAGAAGGAGATCCTCCACCACAGGTACTAATTACTTGTTTCTATTTCTCAACTTCTTAGGAGGTGTTTGAGGTGGAGAGAATTTCTCAAATATGATAATGAATTTGTTGAAATGATCCATTTAAAACGGTATTTCCAATTTTCATGGCAGGAACCTGAGGATGTAGGACCAACCAGACTGGTTGGATACTTTCTAGCGGGATGGATACTGCTAGCATTTGTGTTATCAATTGGACTAAACTTATAG

Protein sequence

>MS.gene42682.t1

MALSSTTTTTICIPSNNTPSKLGFSTNNIVFSVKQARKLCIRSSSENSAGSTEVESESSIELPKESSSLISVLNVERALRGIPITDADHYGRLGLPRGCPYDMVPIAYNNKVRELESQGLEEDILNKKLELLRESYTIMSSPDERRMYDWSLSREDNTDTYVWPFEVDVSVLQEGDPPPQEPEDVGPTRLVGYFLAGWILLAFVLSIGLNL