AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene34935


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene34935.t1 AT1G70760 51.813 193 84 5 4 196 6 189 1.49e-56 177

Find 46 sgRNAs with CRISPR-Local

Find 81 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAAGAACTTTGGTGGATTT+TGG 0.186030 4.1:-16581401 MS.gene34935:CDS
GAGAGAACGAGATGAATAAT+AGG 0.193558 4.1:+16581940 None:intergenic
CAAAGCCAGAACAAGATTAT+TGG 0.194951 4.1:+16581246 None:intergenic
CCAATGGGCAGTGCCAAATT+TGG 0.221494 4.1:+16581666 None:intergenic
GGAAGAGGAGGTAGAGCTTT+TGG 0.286436 4.1:+16581962 None:intergenic
CAGTCTAAAGAAACCAAATT+TGG 0.296905 4.1:-16581679 MS.gene34935:CDS
TGATAGGATCATTTGCCTTC+TGG 0.313169 4.1:+16581700 None:intergenic
TTTGGTTTCTTTAGACTGAT+AGG 0.318596 4.1:+16581684 None:intergenic
TGCAGCCAATAATCTTGTTC+TGG 0.318687 4.1:-16581251 MS.gene34935:intron
CAATAATCTTGTTCTGGCTT+TGG 0.325939 4.1:-16581245 MS.gene34935:CDS
TGTGATGTAGGCTCATTGTT+TGG 0.328228 4.1:-16581046 MS.gene34935:intron
GGGTACCGTTTCTAAACTTC+AGG 0.341388 4.1:-16581025 MS.gene34935:CDS
TGGAGCACTTCTCTTGGCCT+TGG 0.343783 4.1:-16581646 MS.gene34935:intron
AAATGAAGACAAACATGAAT+TGG 0.365498 4.1:+16581184 None:intergenic
GATGGCTAGTCATAATCTTC+AGG 0.365633 4.1:+16580906 None:intergenic
TAATTATTACATGTAGTGAT+TGG 0.389402 4.1:+16581861 None:intergenic
GCCCATTGGAGCACTTCTCT+TGG 0.394201 4.1:-16581652 MS.gene34935:CDS
GTGATGTAGGCTCATTGTTT+GGG 0.399295 4.1:-16581045 MS.gene34935:intron
TATTACATGTAGTGATTGGT+TGG 0.418115 4.1:+16581865 None:intergenic
AGAGAACGAGATGAATAATA+GGG 0.420219 4.1:+16581941 None:intergenic
ATAATAATCCGCAAGAACTT+TGG 0.423897 4.1:-16581411 MS.gene34935:CDS
TGTTCTGGCTTTGGAAAAGA+TGG 0.430184 4.1:-16581236 MS.gene34935:CDS
GACCAAGGATACTCCATGTC+TGG 0.435462 4.1:+16580987 None:intergenic
TAATCTTCAGGATCAGCAAA+AGG 0.448767 4.1:+16580918 None:intergenic
CCAAATTTGGCACTGCCCAT+TGG 0.471352 4.1:-16581666 MS.gene34935:CDS
CGAGATGAATAATAGGGAAG+AGG 0.479300 4.1:+16581947 None:intergenic
ATCCAGACATGGAGTATCCT+TGG 0.501281 4.1:-16580989 MS.gene34935:CDS
ATAATCCGCAAGAACTTTGG+TGG 0.502309 4.1:-16581408 MS.gene34935:CDS
GGCCAAGAGAAGTGCTCCAA+TGG 0.507470 4.1:+16581650 None:intergenic
GATGAATAATAGGGAAGAGG+AGG 0.518891 4.1:+16581950 None:intergenic
CCAGTGACTGCTAATGCTGC+TGG 0.526137 4.1:+16581439 None:intergenic
AGTAAAATGCAGCAGCCAGA+AGG 0.537147 4.1:-16581715 MS.gene34935:CDS
CCAGCAGCATTAGCAGTCAC+TGG 0.537269 4.1:-16581439 MS.gene34935:CDS
AACTTCCTGAAGTTTAGAAA+CGG 0.540412 4.1:+16581020 None:intergenic
TACTCCATGTCTGGATCCCG+TGG 0.543899 4.1:+16580996 None:intergenic
GCCAAGAGAAGTGCTCCAAT+GGG 0.543924 4.1:+16581651 None:intergenic
AATCTTCAGGATCAGCAAAA+GGG 0.561326 4.1:+16580919 None:intergenic
TAAACTTCAGGAAGTTCCCA+CGG 0.579936 4.1:-16581013 MS.gene34935:CDS
AAACTTCAGGAAGTTCCCAC+GGG 0.581934 4.1:-16581012 MS.gene34935:CDS
AAAATCCACCAAAGTTCTTG+CGG 0.588377 4.1:+16581403 None:intergenic
GTTCCCACGGGATCCAGACA+TGG 0.595079 4.1:-16581000 MS.gene34935:CDS
TGACTGCTAATGCTGCTGGT+TGG 0.620328 4.1:+16581443 None:intergenic
TAAAACTTACTGGCCCAACA+AGG 0.670047 4.1:+16581347 None:intergenic
GGATCATCAGGTATAGACCA+AGG 0.670305 4.1:+16580972 None:intergenic
ACTCCATGTCTGGATCCCGT+GGG 0.684029 4.1:+16580997 None:intergenic
ATGGTACAGAAGAAAGCTTG+CGG 0.727997 4.1:-16581217 MS.gene34935:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTACTAAAATAAATATTTAA+TGG - chr4.1:16581333-16581352 MS.gene34935:intron 10.0%
!! CAATTGGATAATTAATTAAT+GGG + chr4.1:16581160-16581179 None:intergenic 15.0%
!! AAAAAATATGACTAGAGAAT+TGG + chr4.1:16581088-16581107 None:intergenic 20.0%
!! ATTTAATGGCAAAATATTAG+AGG - chr4.1:16581347-16581366 MS.gene34935:intron 20.0%
!! GCAATTGGATAATTAATTAA+TGG + chr4.1:16581161-16581180 None:intergenic 20.0%
!! TAATTATTACATGTAGTGAT+TGG + chr4.1:16581042-16581061 None:intergenic 20.0%
!! TTGTAAATACTTATAAGAGA+AGG + chr4.1:16581761-16581780 None:intergenic 20.0%
!! TTTAATGGCAAAATATTAGA+GGG - chr4.1:16581348-16581367 MS.gene34935:intron 20.0%
!!! GATAATTAATTAATGGGTTA+AGG + chr4.1:16581154-16581173 None:intergenic 20.0%
!!! TATGCATTTCTTATTTTTGA+AGG + chr4.1:16581952-16581971 None:intergenic 20.0%
! AAATGAAGACAAACATGAAT+TGG + chr4.1:16581719-16581738 None:intergenic 25.0%
! GATGTGATATTAAAACTTAC+TGG + chr4.1:16581566-16581585 None:intergenic 25.0%
!! TATATACTGTCTCTAAGTTT+TGG - chr4.1:16581377-16581396 MS.gene34935:CDS 25.0%
AACTTCCTGAAGTTTAGAAA+CGG + chr4.1:16581883-16581902 None:intergenic 30.0%
AATATGACTAGAGAATTGGT+TGG + chr4.1:16581084-16581103 None:intergenic 30.0%
AGAGAACGAGATGAATAATA+GGG + chr4.1:16580962-16580981 None:intergenic 30.0%
AGAGTATAATAATCTCACCA+AGG + chr4.1:16581274-16581293 None:intergenic 30.0%
AGTGTGTTAACATAGACAAA+AGG - chr4.1:16581414-16581433 MS.gene34935:CDS 30.0%
ATAATAATCCGCAAGAACTT+TGG - chr4.1:16581489-16581508 MS.gene34935:intron 30.0%
CAAAAGGTGATTATTTGAAC+AGG - chr4.1:16581430-16581449 MS.gene34935:CDS 30.0%
CAGTCTAAAGAAACCAAATT+TGG - chr4.1:16581221-16581240 MS.gene34935:CDS 30.0%
TAGTGATATAAAGTGTAGCA+AGG + chr4.1:16581316-16581335 None:intergenic 30.0%
TATTACATGTAGTGATTGGT+TGG + chr4.1:16581038-16581057 None:intergenic 30.0%
! TCTTTTTCTACTTCCTTGTT+GGG - chr4.1:16581540-16581559 MS.gene34935:intron 30.0%
! TGCATTTTACTATCTGCAAT+TGG + chr4.1:16581176-16581195 None:intergenic 30.0%
! TTCTTTTTCTACTTCCTTGT+TGG - chr4.1:16581539-16581558 MS.gene34935:intron 30.0%
! TTTGGTTTCTTTAGACTGAT+AGG + chr4.1:16581219-16581238 None:intergenic 30.0%
!! TTTCGTTTTTCTTGTGATGT+AGG - chr4.1:16581842-16581861 MS.gene34935:intron 30.0%
!!! CTTATTTTTGAAGGATCATC+AGG + chr4.1:16581943-16581962 None:intergenic 30.0%
AAAATCCACCAAAGTTCTTG+CGG + chr4.1:16581500-16581519 None:intergenic 35.0%
AATCTTCAGGATCAGCAAAA+GGG + chr4.1:16581984-16582003 None:intergenic 35.0%
ACTTTAATGTGCATGGTGAT+TGG - chr4.1:16581794-16581813 MS.gene34935:intron 35.0%
CAAAGCCAGAACAAGATTAT+TGG + chr4.1:16581657-16581676 None:intergenic 35.0%
GAGAGAACGAGATGAATAAT+AGG + chr4.1:16580963-16580982 None:intergenic 35.0%
GCAACTTACTTTAATGTGCA+TGG - chr4.1:16581787-16581806 MS.gene34935:intron 35.0%
TAATCTTCAGGATCAGCAAA+AGG + chr4.1:16581985-16582004 None:intergenic 35.0%
! GAGAGCATAGTGTATTAACA+GGG - chr4.1:16581612-16581631 MS.gene34935:intron 35.0%
! TGAGAGCATAGTGTATTAAC+AGG - chr4.1:16581611-16581630 MS.gene34935:intron 35.0%
!! CAATAATCTTGTTCTGGCTT+TGG - chr4.1:16581655-16581674 MS.gene34935:CDS 35.0%
!! GAGTAGTAGTAGTGTTTTTG+TGG + chr4.1:16581012-16581031 None:intergenic 35.0%
!! GTAGTAGTGTTTTTGTGGAA+TGG + chr4.1:16581007-16581026 None:intergenic 35.0%
ATAATCCGCAAGAACTTTGG+TGG - chr4.1:16581492-16581511 MS.gene34935:intron 40.0%
ATGACTAGAGAATTGGTTGG+TGG + chr4.1:16581081-16581100 None:intergenic 40.0%
ATGGTACAGAAGAAAGCTTG+CGG - chr4.1:16581683-16581702 MS.gene34935:CDS 40.0%
CGAGATGAATAATAGGGAAG+AGG + chr4.1:16580956-16580975 None:intergenic 40.0%
GATGAATAATAGGGAAGAGG+AGG + chr4.1:16580953-16580972 None:intergenic 40.0%
GTGATGTAGGCTCATTGTTT+GGG - chr4.1:16581855-16581874 MS.gene34935:intron 40.0%
TAAAACTTACTGGCCCAACA+AGG + chr4.1:16581556-16581575 None:intergenic 40.0%
TAAACTTCAGGAAGTTCCCA+CGG - chr4.1:16581887-16581906 MS.gene34935:CDS 40.0%
TGATAGGATCATTTGCCTTC+TGG + chr4.1:16581203-16581222 None:intergenic 40.0%
TGCAGCCAATAATCTTGTTC+TGG - chr4.1:16581649-16581668 MS.gene34935:CDS 40.0%
TGTGATGTAGGCTCATTGTT+TGG - chr4.1:16581854-16581873 MS.gene34935:intron 40.0%
!! TGTTCTGGCTTTGGAAAAGA+TGG - chr4.1:16581664-16581683 MS.gene34935:CDS 40.0%
!!! GCAAGAACTTTGGTGGATTT+TGG - chr4.1:16581499-16581518 MS.gene34935:intron 40.0%
!!! TGGTGGATTTTGGCACAATT+GGG - chr4.1:16581509-16581528 MS.gene34935:intron 40.0%
!!! TTGGTGGATTTTGGCACAAT+TGG - chr4.1:16581508-16581527 MS.gene34935:intron 40.0%
AAACTTCAGGAAGTTCCCAC+GGG - chr4.1:16581888-16581907 MS.gene34935:CDS 45.0%
AGTAAAATGCAGCAGCCAGA+AGG - chr4.1:16581185-16581204 MS.gene34935:CDS 45.0%
GGATCATCAGGTATAGACCA+AGG + chr4.1:16581931-16581950 None:intergenic 45.0%
! ATCCAGACATGGAGTATCCT+TGG - chr4.1:16581911-16581930 MS.gene34935:CDS 45.0%
! GGGTACCGTTTCTAAACTTC+AGG - chr4.1:16581875-16581894 MS.gene34935:CDS 45.0%
!! GGTGGATTTTGGCACAATTG+GGG - chr4.1:16581510-16581529 MS.gene34935:intron 45.0%
!! GTGGATTTTGGCACAATTGG+GGG - chr4.1:16581511-16581530 MS.gene34935:intron 45.0%
!!! GAGGTAGAGCTTTTGGAACA+TGG + chr4.1:16580934-16580953 None:intergenic 45.0%
!!! GTAGAGCTTTTGGAACATGG+AGG + chr4.1:16580931-16580950 None:intergenic 45.0%
CCAAATTTGGCACTGCCCAT+TGG - chr4.1:16581234-16581253 MS.gene34935:CDS 50.0%
CCAATGGGCAGTGCCAAATT+TGG + chr4.1:16581237-16581256 None:intergenic 50.0%
GACCAAGGATACTCCATGTC+TGG + chr4.1:16581916-16581935 None:intergenic 50.0%
TGACTGCTAATGCTGCTGGT+TGG + chr4.1:16581460-16581479 None:intergenic 50.0%
! GGAAGAGGAGGTAGAGCTTT+TGG + chr4.1:16580941-16580960 None:intergenic 50.0%
!! GCCAAGAGAAGTGCTCCAAT+GGG + chr4.1:16581252-16581271 None:intergenic 50.0%
ACTCCATGTCTGGATCCCGT+GGG + chr4.1:16581906-16581925 None:intergenic 55.0%
CCAGCAGCATTAGCAGTCAC+TGG - chr4.1:16581461-16581480 MS.gene34935:intron 55.0%
CCAGTGACTGCTAATGCTGC+TGG + chr4.1:16581464-16581483 None:intergenic 55.0%
TACTCCATGTCTGGATCCCG+TGG + chr4.1:16581907-16581926 None:intergenic 55.0%
! AAGAGAAGGTGCACTCACGC+TGG + chr4.1:16581747-16581766 None:intergenic 55.0%
! AGAGAAGGTGCACTCACGCT+GGG + chr4.1:16581746-16581765 None:intergenic 55.0%
! GCCCATTGGAGCACTTCTCT+TGG - chr4.1:16581248-16581267 MS.gene34935:CDS 55.0%
! TGGAGCACTTCTCTTGGCCT+TGG - chr4.1:16581254-16581273 MS.gene34935:intron 55.0%
!! GGCCAAGAGAAGTGCTCCAA+TGG + chr4.1:16581253-16581272 None:intergenic 55.0%
GTTCCCACGGGATCCAGACA+TGG - chr4.1:16581900-16581919 MS.gene34935:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 16580911 16582011 16580911 ID=MS.gene34935
chr4.1 mRNA 16580911 16582011 16580911 ID=MS.gene34935.t1;Parent=MS.gene34935
chr4.1 exon 16581871 16582011 16581871 ID=MS.gene34935.t1.exon1;Parent=MS.gene34935.t1
chr4.1 CDS 16581871 16582011 16581871 ID=cds.MS.gene34935.t1;Parent=MS.gene34935.t1
chr4.1 exon 16581647 16581739 16581647 ID=MS.gene34935.t1.exon2;Parent=MS.gene34935.t1
chr4.1 CDS 16581647 16581739 16581647 ID=cds.MS.gene34935.t1;Parent=MS.gene34935.t1
chr4.1 exon 16581357 16581470 16581357 ID=MS.gene34935.t1.exon3;Parent=MS.gene34935.t1
chr4.1 CDS 16581357 16581470 16581357 ID=cds.MS.gene34935.t1;Parent=MS.gene34935.t1
chr4.1 exon 16581174 16581268 16581174 ID=MS.gene34935.t1.exon4;Parent=MS.gene34935.t1
chr4.1 CDS 16581174 16581268 16581174 ID=cds.MS.gene34935.t1;Parent=MS.gene34935.t1
chr4.1 exon 16580911 16581058 16580911 ID=MS.gene34935.t1.exon5;Parent=MS.gene34935.t1
chr4.1 CDS 16580911 16581058 16580911 ID=cds.MS.gene34935.t1;Parent=MS.gene34935.t1
Gene Sequence

>MS.gene34935

ATGAGCTGCTCATTGAGCCTCCATGTTCCAAAAGCTCTACCTCCTCTTCCCTATTATTCATCTCGTTCTCTCTTCATTGCTTTTAAACACAAACCATTCCACAAAAACACTACTACTACTCTTTCCAACCAATCACTACATGTAATAATTAATTTGCTTTTCTTATTCCACCAACCAATTCTCTAGTCATATTTTTTAGTTTGCTTCTTTCATTGTATATTATATTAACTAATTATCTAACCTTAACCCATTAATTAATTATCCAATTGCAGATAGTAAAATGCAGCAGCCAGAAGGCAAATGATCCTATCAGTCTAAAGAAACCAAATTTGGCACTGCCCATTGGAGCACTTCTCTTGGCCTTGGTGAGATTATTATACTCTTTGTACATAGAAATTCAATCCTTGCTACACTTTATATCACTACTAAAATAAATATTTAATGGCAAAATATTAGAGGGATATTTTATATACTGTCTCTAAGTTTTGGTTGTTATTTTATATAGTGTGTTAACATAGACAAAAGGTGATTATTTGAACAGGCTGACCAACCAGCAGCATTAGCAGTCACTGGATTAAATAATAATCCGCAAGAACTTTGGTGGATTTTGGCACAATTGGGGGTTGTATTCTTTTTCTACTTCCTTGTTGGGCCAGTAAGTTTTAATATCACATCTTATGAAGTTTGACATTATATATAGTGAGAGCATAGTGTATTAACAGGGAGAATTATAATTACTGCAGCCAATAATCTTGTTCTGGCTTTGGAAAAGATGGTACAGAAGAAAGCTTGCGGAGATGTATTTCCAATTCATGTTTGTCTTCATTTTCTTCCCAGCGTGAGTGCACCTTCTCTTATAAGTATTTACAATTTGATGCAACTTACTTTAATGTGCATGGTGATTGGATCTCTCTCTTCTTTTAATTACTAATTTCGTTTTTCTTGTGATGTAGGCTCATTGTTTGGGTACCGTTTCTAAACTTCAGGAAGTTCCCACGGGATCCAGACATGGAGTATCCTTGGTCTATACCTGATGATCCTTCAAAAATAAGAAATGCATACTATAAGTACCCTTTTGCTGATCCTGAAGATTATGACTAG

Protein sequence

>MS.gene34935.t1

MSCSLSLHVPKALPPLPYYSSRSLFIAFKHKPFHKNTTTTLSNQSLHIVKCSSQKANDPISLKKPNLALPIGALLLALADQPAALAVTGLNNNPQELWWILAQLGVVFFFYFLVGPPIILFWLWKRWYRRKLAEMYFQFMFVFIFFPALIVWVPFLNFRKFPRDPDMEYPWSIPDDPSKIRNAYYKYPFADPEDYD