AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene83941


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene83941.t1 MTR_5g038970 94.472 199 10 1 1 198 1 199 6.07e-135 376
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene83941.t1 AT1G14345 78.417 139 28 2 61 198 59 196 1.86e-76 228

Find 69 sgRNAs with CRISPR-Local

Find 75 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAGAAGAATTCTCAGGTTT+TGG 0.237091 5.4:-28971432 MS.gene83941:CDS
CAGGTTGTTCCTAGGTTTAT+TGG 0.243976 5.4:-28971557 MS.gene83941:CDS
CTAAGAGTGAGTGTTATTTC+AGG 0.245187 5.4:-28971632 MS.gene83941:CDS
TGTGGATGTGGTAGGGGTTG+TGG 0.247060 5.4:+28971820 None:intergenic
GTGCACTTTACCTACGTTAC+AGG 0.269722 5.4:-28971664 MS.gene83941:CDS
GTGGATGTGGTAGGGGTTGT+GGG 0.271455 5.4:+28971821 None:intergenic
GGTGTGGTTGTGGTGGTTGA+TGG 0.281173 5.4:+28971934 None:intergenic
CTGAAATAACACTCACTCTT+AGG 0.291915 5.4:+28971633 None:intergenic
GGGGTTGTGGGCTTGTTTGT+TGG 0.299097 5.4:+28971833 None:intergenic
TTTACGGTTGTGGGTGGAGG+TGG 0.315642 5.4:+28971886 None:intergenic
TTCCTAGGTTTATTGGTGAA+TGG 0.317822 5.4:-28971550 MS.gene83941:CDS
GGGGTTGGAGGGTGTGGTTG+TGG 0.332045 5.4:+28971924 None:intergenic
TGGTTGATGGTGGTGGTTGT+TGG 0.333417 5.4:+28971947 None:intergenic
GTTGGAGGGTGTGGTTGTGG+TGG 0.352309 5.4:+28971927 None:intergenic
GTGGTTGTGTGGATGTGGTA+GGG 0.363059 5.4:+28971813 None:intergenic
TAGTGTTATCAAAGATGTTC+AGG 0.370339 5.4:-28971576 MS.gene83941:CDS
TGTGGTGAGTTTACGGTTGT+GGG 0.371948 5.4:+28971877 None:intergenic
AAGAAGATGGTTTCTGCATT+GGG 0.372772 5.4:+28971779 None:intergenic
TTGTGGTGAGTTTACGGTTG+TGG 0.379941 5.4:+28971876 None:intergenic
GGTGGTTGTGTGGATGTGGT+AGG 0.382236 5.4:+28971812 None:intergenic
TCCTAGGTTTATTGGTGAAT+GGG 0.386223 5.4:-28971549 MS.gene83941:CDS
AAATAAAATCAAAAGCCTTT+AGG 0.404905 5.4:+28971398 None:intergenic
TGAAACTGCTGCTAGTGTTA+AGG 0.412622 5.4:+28971688 None:intergenic
GGAAGAGGGGTTGGAGGGTG+TGG 0.412714 5.4:+28971918 None:intergenic
AGTGTTATTTCAGGCTTAAC+GGG 0.414099 5.4:-28971623 MS.gene83941:CDS
GAAACTGCTGCTAGTGTTAA+GGG 0.418053 5.4:+28971689 None:intergenic
GAGTGTTATTTCAGGCTTAA+CGG 0.418536 5.4:-28971624 MS.gene83941:CDS
TGTGGGCTTGTTTGTTGGTG+AGG 0.420960 5.4:+28971838 None:intergenic
TGGTTGTGTGGATGTGGTAG+GGG 0.423031 5.4:+28971814 None:intergenic
TTAGCAATTTCTCTAAACTT+AGG 0.423972 5.4:+28971473 None:intergenic
TAAGTATTGTTTGTCTATGA+AGG 0.424827 5.4:-28971453 MS.gene83941:CDS
TTACGGTTGTGGGTGGAGGT+GGG 0.431500 5.4:+28971887 None:intergenic
TACTCGGCTTCACTCTTGTA+TGG 0.433021 5.4:-28971715 MS.gene83941:CDS
GAGTTTACGGTTGTGGGTGG+AGG 0.435485 5.4:+28971883 None:intergenic
TTGATGGTGGTGGTTGTTGG+TGG 0.448728 5.4:+28971950 None:intergenic
TTGGATCAATCTGGTCCTAA+AGG 0.450236 5.4:-28971413 MS.gene83941:CDS
ATGGTACAAAGGTTGATCTT+AGG 0.451687 5.4:-28971502 MS.gene83941:CDS
GAAGAAGATGGTTTCTGCAT+TGG 0.454026 5.4:+28971778 None:intergenic
GTTTGATGAGGAAGAGGGGT+TGG 0.457551 5.4:+28971909 None:intergenic
AGGTGGTGGTTGTGTGGATG+TGG 0.459887 5.4:+28971808 None:intergenic
GTGGTTGTGGTGGTTGATGG+TGG 0.462739 5.4:+28971937 None:intergenic
TGATGAGGAAGAGGGGTTGG+AGG 0.465797 5.4:+28971912 None:intergenic
ATTGTTATTACATTAAAAGA+TGG 0.465933 5.4:-28971521 MS.gene83941:CDS
GTTGTGGTGGTTGATGGTGG+TGG 0.466674 5.4:+28971940 None:intergenic
GGTGGGTTTGATGAGGAAGA+GGG 0.474274 5.4:+28971904 None:intergenic
GATGAGGAAGAGGGGTTGGA+GGG 0.483645 5.4:+28971913 None:intergenic
TATGAAGGAGAAGAATTCTC+AGG 0.492519 5.4:-28971438 MS.gene83941:CDS
GGGTGGAGGTGGGTTTGATG+AGG 0.494426 5.4:+28971897 None:intergenic
AAGATGTTCAGGTTGTTCCT+AGG 0.508304 5.4:-28971565 MS.gene83941:CDS
CTCATCACCAACCTCCTACT+CGG 0.528547 5.4:-28971731 MS.gene83941:CDS
AGGTGGGTTTGATGAGGAAG+AGG 0.533100 5.4:+28971903 None:intergenic
GGTGAGTTTACGGTTGTGGG+TGG 0.544615 5.4:+28971880 None:intergenic
TATTTCAGGCTTAACGGGTG+AGG 0.547577 5.4:-28971618 MS.gene83941:CDS
AGCTCCACCGTCGAAGAAGA+TGG 0.550541 5.4:+28971766 None:intergenic
TACATTAAAAGATGGTACAA+AGG 0.555819 5.4:-28971513 MS.gene83941:CDS
GAAACCATCTTCTTCGACGG+TGG 0.561109 5.4:-28971770 MS.gene83941:CDS
CGAGTAGGAGGTTGGTGATG+AGG 0.561148 5.4:+28971732 None:intergenic
CCTAGGTTTATTGGTGAATG+GGG 0.564165 5.4:-28971548 MS.gene83941:CDS
GTGGGTTTGATGAGGAAGAG+GGG 0.565343 5.4:+28971905 None:intergenic
CAAGAGTGAAGCCGAGTAGG+AGG 0.565864 5.4:+28971720 None:intergenic
TTCGACGGTGGAGCTCACTA+CGG 0.570521 5.4:-28971758 MS.gene83941:CDS
AGTGAAGCCGAGTAGGAGGT+TGG 0.574847 5.4:+28971724 None:intergenic
AATAACACTCACTCTTAGGT+TGG 0.579943 5.4:+28971637 None:intergenic
ATACAAGAGTGAAGCCGAGT+AGG 0.584381 5.4:+28971717 None:intergenic
GCAGAAACCATCTTCTTCGA+CGG 0.614487 5.4:-28971773 MS.gene83941:CDS
GGTTGGTGAACCTGTAACGT+AGG 0.615503 5.4:+28971654 None:intergenic
GTTGTTGGTGGTGATAAGAG+AGG 0.626805 5.4:+28971962 None:intergenic
TTGTTGGTGAGGATGAGACG+TGG 0.634180 5.4:+28971849 None:intergenic
CCCCATTCACCAATAAACCT+AGG 0.651079 5.4:+28971548 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTGTTATTACATTAAAAGA+TGG - chr5.4:28971865-28971884 MS.gene83941:CDS 15.0%
! TACATTAAAAGATGGTACAA+AGG - chr5.4:28971873-28971892 MS.gene83941:CDS 25.0%
! TTAGCAATTTCTCTAAACTT+AGG + chr5.4:28971916-28971935 None:intergenic 25.0%
!! TAAGTATTGTTTGTCTATGA+AGG - chr5.4:28971933-28971952 MS.gene83941:CDS 25.0%
TAGTGTTATCAAAGATGTTC+AGG - chr5.4:28971810-28971829 MS.gene83941:CDS 30.0%
AAGAAGATGGTTTCTGCATT+GGG + chr5.4:28971610-28971629 None:intergenic 35.0%
AATAACACTCACTCTTAGGT+TGG + chr5.4:28971752-28971771 None:intergenic 35.0%
CTGAAATAACACTCACTCTT+AGG + chr5.4:28971756-28971775 None:intergenic 35.0%
TATGAAGGAGAAGAATTCTC+AGG - chr5.4:28971948-28971967 MS.gene83941:CDS 35.0%
TCCTAGGTTTATTGGTGAAT+GGG - chr5.4:28971837-28971856 MS.gene83941:CDS 35.0%
TTCCTAGGTTTATTGGTGAA+TGG - chr5.4:28971836-28971855 MS.gene83941:CDS 35.0%
! ATGGTACAAAGGTTGATCTT+AGG - chr5.4:28971884-28971903 MS.gene83941:CDS 35.0%
!! AGTGTTATTTCAGGCTTAAC+GGG - chr5.4:28971763-28971782 MS.gene83941:CDS 35.0%
!! CTAAGAGTGAGTGTTATTTC+AGG - chr5.4:28971754-28971773 MS.gene83941:CDS 35.0%
!! GAGTGTTATTTCAGGCTTAA+CGG - chr5.4:28971762-28971781 MS.gene83941:CDS 35.0%
CAGGTTGTTCCTAGGTTTAT+TGG - chr5.4:28971829-28971848 MS.gene83941:CDS 40.0%
CCTAGGTTTATTGGTGAATG+GGG - chr5.4:28971838-28971857 MS.gene83941:CDS 40.0%
GAAACTGCTGCTAGTGTTAA+GGG + chr5.4:28971700-28971719 None:intergenic 40.0%
GAAGAAGATGGTTTCTGCAT+TGG + chr5.4:28971611-28971630 None:intergenic 40.0%
TGAAACTGCTGCTAGTGTTA+AGG + chr5.4:28971701-28971720 None:intergenic 40.0%
TTGGATCAATCTGGTCCTAA+AGG - chr5.4:28971973-28971992 MS.gene83941:CDS 40.0%
! GGAGAAGAATTCTCAGGTTT+TGG - chr5.4:28971954-28971973 MS.gene83941:CDS 40.0%
!! AAGATGTTCAGGTTGTTCCT+AGG - chr5.4:28971821-28971840 MS.gene83941:CDS 40.0%
!! GGAGTTTTGTGGTGAGTTTA+CGG + chr5.4:28971519-28971538 None:intergenic 40.0%
!!! TCTCAGGTTTTGGATCAATC+TGG - chr5.4:28971964-28971983 MS.gene83941:CDS 40.0%
ATACAAGAGTGAAGCCGAGT+AGG + chr5.4:28971672-28971691 None:intergenic 45.0%
CCCCATTCACCAATAAACCT+AGG + chr5.4:28971841-28971860 None:intergenic 45.0%
GCAGAAACCATCTTCTTCGA+CGG - chr5.4:28971613-28971632 MS.gene83941:CDS 45.0%
GTGCACTTTACCTACGTTAC+AGG - chr5.4:28971722-28971741 MS.gene83941:CDS 45.0%
TACTCGGCTTCACTCTTGTA+TGG - chr5.4:28971671-28971690 MS.gene83941:CDS 45.0%
TGTGGTGAGTTTACGGTTGT+GGG + chr5.4:28971512-28971531 None:intergenic 45.0%
TTGTGGTGAGTTTACGGTTG+TGG + chr5.4:28971513-28971532 None:intergenic 45.0%
! GTTTCTGCATTGGGCTTTTG+AGG + chr5.4:28971601-28971620 None:intergenic 45.0%
! TATTTCAGGCTTAACGGGTG+AGG - chr5.4:28971768-28971787 MS.gene83941:CDS 45.0%
!! GTTGTTGGTGGTGATAAGAG+AGG + chr5.4:28971427-28971446 None:intergenic 45.0%
CTCATCACCAACCTCCTACT+CGG - chr5.4:28971655-28971674 MS.gene83941:CDS 50.0%
GAAACCATCTTCTTCGACGG+TGG - chr5.4:28971616-28971635 MS.gene83941:CDS 50.0%
GTGGGTTTGATGAGGAAGAG+GGG + chr5.4:28971484-28971503 None:intergenic 50.0%
GTGGTTGTGTGGATGTGGTA+GGG + chr5.4:28971576-28971595 None:intergenic 50.0%
GTTTGATGAGGAAGAGGGGT+TGG + chr5.4:28971480-28971499 None:intergenic 50.0%
TGGTTGTGTGGATGTGGTAG+GGG + chr5.4:28971575-28971594 None:intergenic 50.0%
! AGGTGGGTTTGATGAGGAAG+AGG + chr5.4:28971486-28971505 None:intergenic 50.0%
! GGATGAGACGTGGAGTTTTG+TGG + chr5.4:28971530-28971549 None:intergenic 50.0%
! GGTGGGTTTGATGAGGAAGA+GGG + chr5.4:28971485-28971504 None:intergenic 50.0%
! TGTGGGCTTGTTTGTTGGTG+AGG + chr5.4:28971551-28971570 None:intergenic 50.0%
! TTGTTGGTGAGGATGAGACG+TGG + chr5.4:28971540-28971559 None:intergenic 50.0%
!! GGTTGGTGAACCTGTAACGT+AGG + chr5.4:28971735-28971754 None:intergenic 50.0%
!! TCTGCATTGGGCTTTTGAGG+TGG + chr5.4:28971598-28971617 None:intergenic 50.0%
!! TGGTTGATGGTGGTGGTTGT+TGG + chr5.4:28971442-28971461 None:intergenic 50.0%
!! TTGATGGTGGTGGTTGTTGG+TGG + chr5.4:28971439-28971458 None:intergenic 50.0%
!!! CTTTTGAGGTGGTGGTTGTG+TGG + chr5.4:28971587-28971606 None:intergenic 50.0%
AGCTCCACCGTCGAAGAAGA+TGG + chr5.4:28971623-28971642 None:intergenic 55.0%
AGTGAAGCCGAGTAGGAGGT+TGG + chr5.4:28971665-28971684 None:intergenic 55.0%
CAAGAGTGAAGCCGAGTAGG+AGG + chr5.4:28971669-28971688 None:intergenic 55.0%
GAGTTTACGGTTGTGGGTGG+AGG + chr5.4:28971506-28971525 None:intergenic 55.0%
GATGAGGAAGAGGGGTTGGA+GGG + chr5.4:28971476-28971495 None:intergenic 55.0%
GGTGAGTTTACGGTTGTGGG+TGG + chr5.4:28971509-28971528 None:intergenic 55.0%
GGTGGTTGTGTGGATGTGGT+AGG + chr5.4:28971577-28971596 None:intergenic 55.0%
GGTGTGGTTGTGGTGGTTGA+TGG + chr5.4:28971455-28971474 None:intergenic 55.0%
GTGGATGTGGTAGGGGTTGT+GGG + chr5.4:28971568-28971587 None:intergenic 55.0%
TGATGAGGAAGAGGGGTTGG+AGG + chr5.4:28971477-28971496 None:intergenic 55.0%
TGTGGATGTGGTAGGGGTTG+TGG + chr5.4:28971569-28971588 None:intergenic 55.0%
TTACGGTTGTGGGTGGAGGT+GGG + chr5.4:28971502-28971521 None:intergenic 55.0%
TTCGACGGTGGAGCTCACTA+CGG - chr5.4:28971628-28971647 MS.gene83941:CDS 55.0%
TTTACGGTTGTGGGTGGAGG+TGG + chr5.4:28971503-28971522 None:intergenic 55.0%
! AGGTGGTGGTTGTGTGGATG+TGG + chr5.4:28971581-28971600 None:intergenic 55.0%
! GGGGTTGTGGGCTTGTTTGT+TGG + chr5.4:28971556-28971575 None:intergenic 55.0%
!! CGAGTAGGAGGTTGGTGATG+AGG + chr5.4:28971657-28971676 None:intergenic 55.0%
!! GTGGTTGTGGTGGTTGATGG+TGG + chr5.4:28971452-28971471 None:intergenic 55.0%
!! GTTGTGGTGGTTGATGGTGG+TGG + chr5.4:28971449-28971468 None:intergenic 55.0%
!!! GCATTGGGCTTTTGAGGTGG+TGG + chr5.4:28971595-28971614 None:intergenic 55.0%
! GGGTGGAGGTGGGTTTGATG+AGG + chr5.4:28971492-28971511 None:intergenic 60.0%
! GTTGGAGGGTGTGGTTGTGG+TGG + chr5.4:28971462-28971481 None:intergenic 60.0%
GGAAGAGGGGTTGGAGGGTG+TGG + chr5.4:28971471-28971490 None:intergenic 65.0%
! GGGGTTGGAGGGTGTGGTTG+TGG + chr5.4:28971465-28971484 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 28971406 28972002 28971406 ID=MS.gene83941
chr5.4 mRNA 28971406 28972002 28971406 ID=MS.gene83941.t1;Parent=MS.gene83941
chr5.4 exon 28971406 28972002 28971406 ID=MS.gene83941.t1.exon1;Parent=MS.gene83941.t1
chr5.4 CDS 28971406 28972002 28971406 ID=cds.MS.gene83941.t1;Parent=MS.gene83941.t1
Gene Sequence

>MS.gene83941

ATGGCTTGCAATTCTACACCTCTCTTATCACCACCAACAACCACCACCATCAACCACCACAACCACACCCTCCAACCCCTCTTCCTCATCAAACCCACCTCCACCCACAACCGTAAACTCACCACAAAACTCCACGTCTCATCCTCACCAACAAACAAGCCCACAACCCCTACCACATCCACACAACCACCACCTCAAAAGCCCAATGCAGAAACCATCTTCTTCGACGGTGGAGCTCACTACGGAGACCTCATCACCAACCTCCTACTCGGCTTCACTCTTGTATGGCTTCCCTTAACACTAGCAGCAGTTTCACGTGCACTTTACCTACGTTACAGGTTCACCAACCTAAGAGTGAGTGTTATTTCAGGCTTAACGGGTGAGGATAGAAGTGACTTTTCATATAGTGTTATCAAAGATGTTCAGGTTGTTCCTAGGTTTATTGGTGAATGGGGTGATATTGTTATTACATTAAAAGATGGTACAAAGGTTGATCTTAGGAGTGTTCCTAAGTTTAGAGAAATTGCTAAGTATTGTTTGTCTATGAAGGAGAAGAATTCTCAGGTTTTGGATCAATCTGGTCCTAAAGGCTTTTGA

Protein sequence

>MS.gene83941.t1

MACNSTPLLSPPTTTTINHHNHTLQPLFLIKPTSTHNRKLTTKLHVSSSPTNKPTTPTTSTQPPPQKPNAETIFFDGGAHYGDLITNLLLGFTLVWLPLTLAAVSRALYLRYRFTNLRVSVISGLTGEDRSDFSYSVIKDVQVVPRFIGEWGDIVITLKDGTKVDLRSVPKFREIAKYCLSMKEKNSQVLDQSGPKGF