AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0411270


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0411270 MtrunA17_Chr3g0102541 88.312 77 9 0 7 83 446 522 3.80e-41 144
Msa0411270 MtrunA17_Chr3g0102541 68.889 45 13 1 66 110 600 643 7.84e-13 63.2
Msa0411270 MtrunA17_Chr3g0102531 83.117 77 13 0 7 83 445 521 2.13e-39 139
Msa0411270 MtrunA17_Chr3g0102531 71.111 45 12 1 66 110 599 642 5.21e-13 63.9
Msa0411270 MtrunA17_Chr3g0102551 73.239 71 19 0 7 77 451 521 1.36e-30 114
Msa0411270 MtrunA17_Chr8g0383701 41.463 82 45 1 7 88 797 875 9.71e-15 68.9
Msa0411270 MtrunA17_Chr2g0296991 46.032 63 34 0 7 69 745 807 1.48e-14 68.2
Msa0411270 MtrunA17_Chr8g0379241 44.444 63 35 0 7 69 689 751 2.55e-14 67.8
Msa0411270 MtrunA17_Chr2g0284211 42.857 63 36 0 7 69 677 739 8.17e-14 66.2
Msa0411270 MtrunA17_Chr1g0201731 46.032 63 34 0 7 69 798 860 1.52e-13 65.5
Msa0411270 MtrunA17_Chr7g0267731 42.857 63 36 0 7 69 686 748 1.94e-13 65.1
Msa0411270 MtrunA17_Chr8g0352231 42.857 63 36 0 7 69 778 840 2.11e-13 65.1
Msa0411270 MtrunA17_Chr1g0183381 43.548 62 35 0 7 68 606 667 3.92e-13 64.3
Msa0411270 MtrunA17_Chr8g0364641 43.750 64 36 0 5 68 677 740 1.15e-12 62.8
Msa0411270 MtrunA17_Chr3g0137951 37.838 74 45 1 7 79 786 859 3.81e-12 61.2
Msa0411270 MtrunA17_Chr4g0073711 40.984 61 36 0 7 67 738 798 3.84e-12 61.2
Msa0411270 MtrunA17_Chr3g0088821 41.935 62 36 0 7 68 778 839 4.20e-12 61.2
Msa0411270 MtrunA17_Chr4g0046401 42.857 70 39 1 7 76 453 521 9.79e-11 57.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0411270 AT1G55850.1 65.217 69 24 0 7 75 443 511 2.54e-26 102
Msa0411270 AT4G39350.1 41.463 82 45 1 7 88 784 862 1.41e-14 68.6
Msa0411270 AT2G21770.1 41.463 82 45 1 7 88 788 866 1.50e-14 68.6
Msa0411270 AT5G44030.2 46.032 63 34 0 7 69 777 839 3.75e-14 67.4
Msa0411270 AT5G44030.1 46.032 63 34 0 7 69 748 810 3.82e-14 67.4
Msa0411270 AT5G44030.3 46.032 63 34 0 7 69 469 531 4.04e-14 67.4
Msa0411270 AT4G32410.1 45.161 62 34 0 7 68 780 841 2.01e-13 65.5
Msa0411270 AT5G17420.1 41.935 62 36 0 7 68 726 787 2.51e-13 65.1
Msa0411270 AT4G24010.1 42.500 80 44 1 7 86 447 524 4.66e-13 64.3
Msa0411270 AT4G18780.1 42.623 61 35 0 7 67 683 743 9.56e-13 63.5
Msa0411270 AT2G25540.1 42.857 63 36 0 7 69 760 822 1.59e-12 62.8
Msa0411270 AT5G64740.1 42.857 63 36 0 7 69 785 847 5.26e-12 61.2
Msa0411270 AT4G23990.1 40.000 75 43 1 7 81 466 538 7.07e-12 60.8
Msa0411270 AT5G09870.1 42.857 63 36 0 7 69 770 832 7.32e-12 60.8
Msa0411270 AT4G23990.2 40.000 75 43 1 7 81 252 324 8.97e-12 60.8
Msa0411270 AT5G05170.1 40.323 62 37 0 7 68 765 826 2.61e-11 59.3
Msa0411270 AT4G15320.2 41.379 58 34 0 7 64 506 563 9.39e-11 57.8

Find 20 sgRNAs with CRISPR-Local

Find 126 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCAAAGTTCAGTCCTATT+TGG 0.223598 3_2:+53485628 Msa0411270:CDS
AGTCCTATTTGGTACGCTTT+TGG 0.262148 3_2:+53485639 Msa0411270:CDS
CCAACAAGGAAAGCTTTCTT+AGG 0.270056 3_2:+53485531 Msa0411270:CDS
TTATGTTGAACAAGTGATTC+TGG 0.278433 3_2:-53485573 None:intergenic
CCTAAGAAAGCTTTCCTTGT+TGG 0.298769 3_2:-53485531 None:intergenic
GAGAAGGATATTATGGAGTT+TGG 0.300136 3_2:+53486373 Msa0411270:CDS
AAGATATGAGAAGGATATTA+TGG 0.313246 3_2:+53486366 Msa0411270:CDS
AATCCAAAAGCGTACCAAAT+AGG 0.362728 3_2:-53485642 None:intergenic
AGTGATTCTGGCAAGGTTGT+TGG 0.401127 3_2:-53485561 None:intergenic
AGTGACAGTAAGACATTTGA+AGG 0.417903 3_2:-53485677 None:intergenic
AAATTTCATAATATCTACTA+AGG 0.468679 3_2:+53486321 Msa0411270:CDS
CAGTAAGACATTTGAAGGCC+TGG 0.486113 3_2:-53485672 None:intergenic
CAGTGTGGAGATGTAATAAC+AGG 0.506505 3_2:+53485465 Msa0411270:CDS
CCTTGTTGGATTATAGTACA+CGG 0.570690 3_2:-53485517 None:intergenic
CACTTGTTCAACATAAGAGA+TGG 0.602865 3_2:+53485580 Msa0411270:CDS
CAACATAAGAGATGGTCTGA+AGG 0.609206 3_2:+53485588 Msa0411270:CDS
TGTTTCACAAAGATATGAGA+AGG 0.620914 3_2:+53486357 Msa0411270:CDS
CCGTGTACTATAATCCAACA+AGG 0.621803 3_2:+53485517 Msa0411270:CDS
TTGAACAAGTGATTCTGGCA+AGG 0.652845 3_2:-53485568 None:intergenic
TATTATATGGTTGTGCAGTG+TGG 0.658058 3_2:+53485450 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTGTTATTAGTAAAATTC+AGG + chr3_2:53485943-53485962 Msa0411270:intron 15.0%
!! AAATTTCATAATATCTACTA+AGG + chr3_2:53486321-53486340 Msa0411270:CDS 15.0%
!!! TATTGTTATTAGTAAAATTC+AGG + chr3_2:53485943-53485962 Msa0411270:intron 15.0%
!! AAATTTCATAATATCTACTA+AGG + chr3_2:53486321-53486340 Msa0411270:CDS 15.0%
!! GGATTATCTATTTTATGCAA+AGG + chr3_2:53485486-53485505 Msa0411270:CDS 25.0%
!! TATCTATTTTATGCAAAGGA+TGG + chr3_2:53485490-53485509 Msa0411270:CDS 25.0%
! TTACTGTCACTATAATATGT+GGG + chr3_2:53485689-53485708 Msa0411270:intron 25.0%
!! ATGATGGAATAATACATAGT+TGG - chr3_2:53485729-53485748 None:intergenic 25.0%
!! TAGTAAAATTTTCATACCTG+TGG - chr3_2:53485789-53485808 None:intergenic 25.0%
!! TATCCTTTAGAAGAGATAAT+TGG + chr3_2:53485918-53485937 Msa0411270:intron 25.0%
! AAACCAATTATCTCTTCTAA+AGG - chr3_2:53485924-53485943 None:intergenic 25.0%
! TTGCAATATAACGTTTATAG+AGG - chr3_2:53486018-53486037 None:intergenic 25.0%
!! TTTTGTTGATACCATGTTAA+AGG + chr3_2:53486278-53486297 Msa0411270:intron 25.0%
!!! ATACCATGTTAAAGGTTTTT+TGG + chr3_2:53486286-53486305 Msa0411270:intron 25.0%
! AATCCAAAAAACCTTTAACA+TGG - chr3_2:53486292-53486311 None:intergenic 25.0%
! AAGATATGAGAAGGATATTA+TGG + chr3_2:53486366-53486385 Msa0411270:CDS 25.0%
!! GGATTATCTATTTTATGCAA+AGG + chr3_2:53485486-53485505 Msa0411270:CDS 25.0%
!! TATCTATTTTATGCAAAGGA+TGG + chr3_2:53485490-53485509 Msa0411270:CDS 25.0%
! TTACTGTCACTATAATATGT+GGG + chr3_2:53485689-53485708 Msa0411270:intron 25.0%
!! ATGATGGAATAATACATAGT+TGG - chr3_2:53485729-53485748 None:intergenic 25.0%
!! TAGTAAAATTTTCATACCTG+TGG - chr3_2:53485789-53485808 None:intergenic 25.0%
!! TATCCTTTAGAAGAGATAAT+TGG + chr3_2:53485918-53485937 Msa0411270:intron 25.0%
! AAACCAATTATCTCTTCTAA+AGG - chr3_2:53485924-53485943 None:intergenic 25.0%
! TTGCAATATAACGTTTATAG+AGG - chr3_2:53486018-53486037 None:intergenic 25.0%
!! TTTTGTTGATACCATGTTAA+AGG + chr3_2:53486278-53486297 Msa0411270:intron 25.0%
!!! ATACCATGTTAAAGGTTTTT+TGG + chr3_2:53486286-53486305 Msa0411270:intron 25.0%
! AATCCAAAAAACCTTTAACA+TGG - chr3_2:53486292-53486311 None:intergenic 25.0%
! AAGATATGAGAAGGATATTA+TGG + chr3_2:53486366-53486385 Msa0411270:CDS 25.0%
TTATGTTGAACAAGTGATTC+TGG - chr3_2:53485576-53485595 None:intergenic 30.0%
TTTCAAAGTTCAGTCCTATT+TGG + chr3_2:53485628-53485647 Msa0411270:CDS 30.0%
CTTACTGTCACTATAATATG+TGG + chr3_2:53485688-53485707 Msa0411270:intron 30.0%
GGAATAATACATAGTTGGAA+AGG - chr3_2:53485724-53485743 None:intergenic 30.0%
CTACATCAGTTCCTATATAT+AGG + chr3_2:53485806-53485825 Msa0411270:intron 30.0%
ATTGATCAGATTTAAGTCCT+TGG + chr3_2:53486113-53486132 Msa0411270:intron 30.0%
!! TGTTTGTTAGAGTTTTTGTG+AGG + chr3_2:53486178-53486197 Msa0411270:intron 30.0%
!! GTTTGTTAGAGTTTTTGTGA+GGG + chr3_2:53486179-53486198 Msa0411270:intron 30.0%
TGGTATCAACAAAAGCAAAA+AGG - chr3_2:53486272-53486291 None:intergenic 30.0%
TGTTTCACAAAGATATGAGA+AGG + chr3_2:53486357-53486376 Msa0411270:CDS 30.0%
TTATGTTGAACAAGTGATTC+TGG - chr3_2:53485576-53485595 None:intergenic 30.0%
TTTCAAAGTTCAGTCCTATT+TGG + chr3_2:53485628-53485647 Msa0411270:CDS 30.0%
CTTACTGTCACTATAATATG+TGG + chr3_2:53485688-53485707 Msa0411270:intron 30.0%
GGAATAATACATAGTTGGAA+AGG - chr3_2:53485724-53485743 None:intergenic 30.0%
CTACATCAGTTCCTATATAT+AGG + chr3_2:53485806-53485825 Msa0411270:intron 30.0%
ATTGATCAGATTTAAGTCCT+TGG + chr3_2:53486113-53486132 Msa0411270:intron 30.0%
!! TGTTTGTTAGAGTTTTTGTG+AGG + chr3_2:53486178-53486197 Msa0411270:intron 30.0%
!! GTTTGTTAGAGTTTTTGTGA+GGG + chr3_2:53486179-53486198 Msa0411270:intron 30.0%
TGGTATCAACAAAAGCAAAA+AGG - chr3_2:53486272-53486291 None:intergenic 30.0%
TGTTTCACAAAGATATGAGA+AGG + chr3_2:53486357-53486376 Msa0411270:CDS 30.0%
CCTTGTTGGATTATAGTACA+CGG - chr3_2:53485520-53485539 None:intergenic 35.0%
CACTTGTTCAACATAAGAGA+TGG + chr3_2:53485580-53485599 Msa0411270:CDS 35.0%
! AATCCAAAAGCGTACCAAAT+AGG - chr3_2:53485645-53485664 None:intergenic 35.0%
AGTGACAGTAAGACATTTGA+AGG - chr3_2:53485680-53485699 None:intergenic 35.0%
! GAGAAAAAGTGAAGGTATGA+TGG - chr3_2:53485745-53485764 None:intergenic 35.0%
TTTCATACCTGTGGAAACAA+GGG - chr3_2:53485780-53485799 None:intergenic 35.0%
! TTTTCATACCTGTGGAAACA+AGG - chr3_2:53485781-53485800 None:intergenic 35.0%
CACAACGAGATCCTATATAT+AGG - chr3_2:53485820-53485839 None:intergenic 35.0%
TATTGCAATCACAATTGCAG+TGG + chr3_2:53486030-53486049 Msa0411270:intron 35.0%
! TATGCAAAAGGTATGAACCA+AGG - chr3_2:53486133-53486152 None:intergenic 35.0%
CCAACTATGACATATGCAAA+AGG - chr3_2:53486145-53486164 None:intergenic 35.0%
! CCTTTTGCATATGTCATAGT+TGG + chr3_2:53486142-53486161 Msa0411270:intron 35.0%
GAGAAGGATATTATGGAGTT+TGG + chr3_2:53486373-53486392 Msa0411270:CDS 35.0%
CCTTGTTGGATTATAGTACA+CGG - chr3_2:53485520-53485539 None:intergenic 35.0%
CACTTGTTCAACATAAGAGA+TGG + chr3_2:53485580-53485599 Msa0411270:CDS 35.0%
! AATCCAAAAGCGTACCAAAT+AGG - chr3_2:53485645-53485664 None:intergenic 35.0%
AGTGACAGTAAGACATTTGA+AGG - chr3_2:53485680-53485699 None:intergenic 35.0%
! GAGAAAAAGTGAAGGTATGA+TGG - chr3_2:53485745-53485764 None:intergenic 35.0%
TTTCATACCTGTGGAAACAA+GGG - chr3_2:53485780-53485799 None:intergenic 35.0%
! TTTTCATACCTGTGGAAACA+AGG - chr3_2:53485781-53485800 None:intergenic 35.0%
CACAACGAGATCCTATATAT+AGG - chr3_2:53485820-53485839 None:intergenic 35.0%
TATTGCAATCACAATTGCAG+TGG + chr3_2:53486030-53486049 Msa0411270:intron 35.0%
! TATGCAAAAGGTATGAACCA+AGG - chr3_2:53486133-53486152 None:intergenic 35.0%
CCAACTATGACATATGCAAA+AGG - chr3_2:53486145-53486164 None:intergenic 35.0%
! CCTTTTGCATATGTCATAGT+TGG + chr3_2:53486142-53486161 Msa0411270:intron 35.0%
GAGAAGGATATTATGGAGTT+TGG + chr3_2:53486373-53486392 Msa0411270:CDS 35.0%
CAGTGTGGAGATGTAATAAC+AGG + chr3_2:53485465-53485484 Msa0411270:CDS 40.0%
CCGTGTACTATAATCCAACA+AGG + chr3_2:53485517-53485536 Msa0411270:CDS 40.0%
CCTAAGAAAGCTTTCCTTGT+TGG - chr3_2:53485534-53485553 None:intergenic 40.0%
CCAACAAGGAAAGCTTTCTT+AGG + chr3_2:53485531-53485550 Msa0411270:CDS 40.0%
TTGAACAAGTGATTCTGGCA+AGG - chr3_2:53485571-53485590 None:intergenic 40.0%
CAACATAAGAGATGGTCTGA+AGG + chr3_2:53485588-53485607 Msa0411270:CDS 40.0%
! AGTCCTATTTGGTACGCTTT+TGG + chr3_2:53485639-53485658 Msa0411270:CDS 40.0%
!! GCTTTTGGATTGATTAGTCC+AGG + chr3_2:53485654-53485673 Msa0411270:CDS 40.0%
! CCTTTGAGGAGAAAAAGTGA+AGG - chr3_2:53485753-53485772 None:intergenic 40.0%
!!! CCTTCACTTTTTCTCCTCAA+AGG + chr3_2:53485750-53485769 Msa0411270:intron 40.0%
TTCAGGCAAATGTAACCATG+TGG + chr3_2:53485960-53485979 Msa0411270:intron 40.0%
! GTTAGAGTTTTTGTGAGGGT+AGG + chr3_2:53486183-53486202 Msa0411270:intron 40.0%
AGGAGGTTACACTTAAAGGT+TGG + chr3_2:53486203-53486222 Msa0411270:intron 40.0%
AGGTTACACTTAAAGGTTGG+TGG + chr3_2:53486206-53486225 Msa0411270:intron 40.0%
!! ACTTAAAGGTTGGTGGAATG+AGG + chr3_2:53486213-53486232 Msa0411270:intron 40.0%
CAGTGTGGAGATGTAATAAC+AGG + chr3_2:53485465-53485484 Msa0411270:CDS 40.0%
CCGTGTACTATAATCCAACA+AGG + chr3_2:53485517-53485536 Msa0411270:CDS 40.0%
CCTAAGAAAGCTTTCCTTGT+TGG - chr3_2:53485534-53485553 None:intergenic 40.0%
CCAACAAGGAAAGCTTTCTT+AGG + chr3_2:53485531-53485550 Msa0411270:CDS 40.0%
TTGAACAAGTGATTCTGGCA+AGG - chr3_2:53485571-53485590 None:intergenic 40.0%
CAACATAAGAGATGGTCTGA+AGG + chr3_2:53485588-53485607 Msa0411270:CDS 40.0%
! AGTCCTATTTGGTACGCTTT+TGG + chr3_2:53485639-53485658 Msa0411270:CDS 40.0%
!! GCTTTTGGATTGATTAGTCC+AGG + chr3_2:53485654-53485673 Msa0411270:CDS 40.0%
! CCTTTGAGGAGAAAAAGTGA+AGG - chr3_2:53485753-53485772 None:intergenic 40.0%
!!! CCTTCACTTTTTCTCCTCAA+AGG + chr3_2:53485750-53485769 Msa0411270:intron 40.0%
TTCAGGCAAATGTAACCATG+TGG + chr3_2:53485960-53485979 Msa0411270:intron 40.0%
! GTTAGAGTTTTTGTGAGGGT+AGG + chr3_2:53486183-53486202 Msa0411270:intron 40.0%
AGGAGGTTACACTTAAAGGT+TGG + chr3_2:53486203-53486222 Msa0411270:intron 40.0%
AGGTTACACTTAAAGGTTGG+TGG + chr3_2:53486206-53486225 Msa0411270:intron 40.0%
!! ACTTAAAGGTTGGTGGAATG+AGG + chr3_2:53486213-53486232 Msa0411270:intron 40.0%
AGTGATTCTGGCAAGGTTGT+TGG - chr3_2:53485564-53485583 None:intergenic 45.0%
CAGTAAGACATTTGAAGGCC+TGG - chr3_2:53485675-53485694 None:intergenic 45.0%
GAAACAAGGGAATGCCTTTG+AGG - chr3_2:53485767-53485786 None:intergenic 45.0%
TGACATCACAACCACACTTG+CGG - chr3_2:53485986-53486005 None:intergenic 45.0%
! AGAGTTTTTGTGAGGGTAGG+AGG + chr3_2:53486186-53486205 Msa0411270:intron 45.0%
GGGTAGGAGGTTACACTTAA+AGG + chr3_2:53486199-53486218 Msa0411270:intron 45.0%
AGTGATTCTGGCAAGGTTGT+TGG - chr3_2:53485564-53485583 None:intergenic 45.0%
CAGTAAGACATTTGAAGGCC+TGG - chr3_2:53485675-53485694 None:intergenic 45.0%
GAAACAAGGGAATGCCTTTG+AGG - chr3_2:53485767-53485786 None:intergenic 45.0%
TGACATCACAACCACACTTG+CGG - chr3_2:53485986-53486005 None:intergenic 45.0%
! AGAGTTTTTGTGAGGGTAGG+AGG + chr3_2:53486186-53486205 Msa0411270:intron 45.0%
GGGTAGGAGGTTACACTTAA+AGG + chr3_2:53486199-53486218 Msa0411270:intron 45.0%
! AGGCATTCCCTTGTTTCCAC+AGG + chr3_2:53485770-53485789 Msa0411270:intron 50.0%
!! AAGGTTGGTGGAATGAGGTG+AGG + chr3_2:53486218-53486237 Msa0411270:intron 50.0%
! AGGCATTCCCTTGTTTCCAC+AGG + chr3_2:53485770-53485789 Msa0411270:intron 50.0%
!! AAGGTTGGTGGAATGAGGTG+AGG + chr3_2:53486218-53486237 Msa0411270:intron 50.0%
TAACCATGTGGCCGCAAGTG+TGG + chr3_2:53485972-53485991 Msa0411270:intron 55.0%
GGTGGAATGAGGTGAGGATG+TGG + chr3_2:53486224-53486243 Msa0411270:intron 55.0%
TAACCATGTGGCCGCAAGTG+TGG + chr3_2:53485972-53485991 Msa0411270:intron 55.0%
GGTGGAATGAGGTGAGGATG+TGG + chr3_2:53486224-53486243 Msa0411270:intron 55.0%
CAACCACACTTGCGGCCACA+TGG - chr3_2:53485978-53485997 None:intergenic 60.0%
CAACCACACTTGCGGCCACA+TGG - chr3_2:53485978-53485997 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 53485456 53486411 53485456 ID=Msa0411270;Name=Msa0411270
chr3_2 mRNA 53485456 53486411 53485456 ID=Msa0411270-mRNA-1;Parent=Msa0411270;Name=Msa0411270-mRNA-1;_AED=0.47;_eAED=0.48;_QI=0|0|0|1|1|1|2|0|113
chr3_2 exon 53485456 53485685 53485456 ID=Msa0411270-mRNA-1:exon:10688;Parent=Msa0411270-mRNA-1
chr3_2 exon 53486300 53486411 53486300 ID=Msa0411270-mRNA-1:exon:10689;Parent=Msa0411270-mRNA-1
chr3_2 CDS 53485456 53485685 53485456 ID=Msa0411270-mRNA-1:cds;Parent=Msa0411270-mRNA-1
chr3_2 CDS 53486300 53486411 53486300 ID=Msa0411270-mRNA-1:cds;Parent=Msa0411270-mRNA-1
Gene Sequence

>Msa0411270

ATGGTTGTGCAGTGTGGAGATGTAATAACAGGATTATCTATTTTATGCAAAGGATGGAAATCCGTGTACTATAATCCAACAAGGAAAGCTTTCTTAGGCTTGTGTCCAACAACCTTGCCAGAATCACTTGTTCAACATAAGAGATGGTCTGAAGGAGAATTTCAAATTCTACTTTCAAAGTTCAGTCCTATTTGGTACGCTTTTGGATTGATTAGTCCAGGCCTTCAAATGTTTTTTGGATTTTCAAATTCAAATTTCATAATATCTACTAAGGTAGCTGAAGAAAATGTTTCACAAAGATATGAGAAGGATATTATGGAGTTTGGAACTCATCTCCAATGA

Protein sequence

>Msa0411270

MVVQCGDVITGLSILCKGWKSVYYNPTRKAFLGLCPTTLPESLVQHKRWSEGEFQILLSKFSPIWYAFGLISPGLQMFFGFSNSNFIISTKVAEENVSQRYEKDIMEFGTHLQ*