AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene42627


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene42627.t1 MTR_3g104980 95.261 211 10 0 1 211 1 211 3.08e-143 398
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene42627.t1 AT5G21430 55.738 183 77 3 32 210 35 217 1.83e-62 194
MS.gene42627.t1 AT5G21430 55.191 183 77 4 32 210 35 216 5.39e-60 187

Find 50 sgRNAs with CRISPR-Local

Find 74 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTAAAGAAGATGATTCTTT+TGG 0.261740 3.4:-91914331 None:intergenic
AGACATACTTAACAAGAAAT+TGG 0.280104 3.4:+91914763 MS.gene42627:CDS
ATTGGAGAGTCAAGGTTTAG+AGG 0.312619 3.4:+91914739 MS.gene42627:CDS
GCCATTTGAGGTTGATGTTT+CGG 0.315689 3.4:+91914982 MS.gene42627:CDS
TAATAACAAGGTTCGAGAAT+TGG 0.322385 3.4:+91914721 MS.gene42627:CDS
CTAGCATTTGTGTTATCAAT+TGG 0.334884 3.4:+91915221 MS.gene42627:CDS
ATTACAGATGCAGATCATTA+TGG 0.366825 3.4:+91914481 MS.gene42627:CDS
TGTGTCTGTGTTATCTTCTC+TGG 0.408544 3.4:-91914951 None:intergenic
ACCTTGTTATTATAAGCAAT+TGG 0.411448 3.4:-91914710 None:intergenic
CACATACGTTTGGCCATTTG+AGG 0.421335 3.4:+91914970 MS.gene42627:CDS
TCATACATTCTTCTTTCATC+CGG 0.438615 3.4:-91914917 None:intergenic
ATGTTTCTGACCATGTCATA+TGG 0.442154 3.4:-91914529 None:intergenic
TTGCTTGGAGTGTTGTTACT+TGG 0.444787 3.4:-91914184 None:intergenic
GTTGAGAATCCTAGCTTGCT+TGG 0.458033 3.4:-91914199 None:intergenic
CTGGTTGGTCCTACATCCTC+AGG 0.458680 3.4:-91915164 None:intergenic
ACCGAAACATCAACCTCAAA+TGG 0.480733 3.4:-91914983 None:intergenic
GCAGATCATTATGGTAGACT+TGG 0.523390 3.4:+91914490 MS.gene42627:CDS
GCAAATTGTAGTGGTGGTGG+TGG 0.527140 3.4:-91914158 None:intergenic
AGCTCTAGTGAAAACTCTGC+AGG 0.533335 3.4:+91914271 MS.gene42627:CDS
TAGAAAGTATCCAACCAGTC+TGG 0.536223 3.4:-91915183 None:intergenic
CTGGTTGGATACTTTCTAGC+GGG 0.536918 3.4:+91915188 MS.gene42627:CDS
ATAACACAGACACATACGTT+TGG 0.539673 3.4:+91914960 MS.gene42627:CDS
ATGAAAGAAGAATGTATGAC+TGG 0.543817 3.4:+91914921 MS.gene42627:CDS
GATGTTTCGGTACTTCAAGA+AGG 0.552371 3.4:+91914995 MS.gene42627:CDS
ATGTCATATGGACATCCTCT+TGG 0.556234 3.4:-91914517 None:intergenic
AGTGGTGGTGGTGGAGGACA+AGG 0.559487 3.4:-91914149 None:intergenic
AATTGTAGTGGTGGTGGTGG+AGG 0.559668 3.4:-91914155 None:intergenic
TTGGATACTTTCTAGCGGGA+TGG 0.565729 3.4:+91915192 MS.gene42627:CDS
GTAATTAGTACCTGTGGTGG+AGG 0.566183 3.4:-91915022 None:intergenic
GTTCGAGAATTGGAGAGTCA+AGG 0.571210 3.4:+91914731 MS.gene42627:CDS
GTAGGACCAACCAGACTGGT+TGG 0.579118 3.4:+91915173 MS.gene42627:CDS
TGAAAACTCTGCAGGTTCAA+CGG 0.580415 3.4:+91914279 MS.gene42627:CDS
CAAGTAATTAGTACCTGTGG+TGG 0.590198 3.4:-91915025 None:intergenic
GGATGTAGGACCAACCAGAC+TGG 0.590754 3.4:+91915169 MS.gene42627:CDS
TGGCAGGAACCTGAGGATGT+AGG 0.592315 3.4:+91915155 MS.gene42627:intron
AACAACACTCCAAGCAAGCT+AGG 0.601547 3.4:+91914190 MS.gene42627:CDS
ATACACTATCATGTCATCGC+CGG 0.604771 3.4:+91914898 MS.gene42627:CDS
GCCAATTGCTTATAATAACA+AGG 0.606196 3.4:+91914709 MS.gene42627:CDS
AATGCAAATTGTAGTGGTGG+TGG 0.612182 3.4:-91914161 None:intergenic
AGGTTCAACGGAGGTTGAGT+CGG 0.620899 3.4:+91914291 MS.gene42627:CDS
AAGTATCCAACCAGTCTGGT+TGG 0.621927 3.4:-91915179 None:intergenic
AGAAGGAGATCCTCCACCAC+AGG 0.633320 3.4:+91915012 MS.gene42627:CDS
ACTTGGAATGCAAATTGTAG+TGG 0.640752 3.4:-91914167 None:intergenic
GGTAGACTTGGCTTACCAAG+AGG 0.643717 3.4:+91914502 MS.gene42627:CDS
TGGAAAACATACGTATGCCA+CGG 0.651444 3.4:-91914381 None:intergenic
TGGAATGCAAATTGTAGTGG+TGG 0.664067 3.4:-91914164 None:intergenic
AAGAGGATGTCCATATGACA+TGG 0.675165 3.4:+91914519 MS.gene42627:CDS
ACTGGTTGGATACTTTCTAG+CGG 0.691428 3.4:+91915187 MS.gene42627:CDS
AACGTCGAAAGAGCTCTCCG+TGG 0.728158 3.4:+91914364 MS.gene42627:CDS
AAACTCTGCAGGTTCAACGG+AGG 0.732304 3.4:+91914282 MS.gene42627:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATAATAACAACAACATTA+TGG - chr3.4:91914404-91914423 None:intergenic 15.0%
!! TTGAAATGATCCATTTAAAA+CGG + chr3.4:91915117-91915136 MS.gene42627:intron 20.0%
!!! ATTAAAGAAGATGATTCTTT+TGG - chr3.4:91914334-91914353 None:intergenic 20.0%
! AGACATACTTAACAAGAAAT+TGG + chr3.4:91914763-91914782 MS.gene42627:CDS 25.0%
! GAATTGAATTGATGAAGATA+TGG + chr3.4:91914444-91914463 MS.gene42627:intron 25.0%
! GTATGATTCCTATACAATTA+AGG - chr3.4:91914882-91914901 None:intergenic 25.0%
! TCGGATTTAATTCATATGAA+AGG + chr3.4:91914558-91914577 MS.gene42627:intron 25.0%
! TGTGTTTGCATCATTATTAT+AGG + chr3.4:91914827-91914846 MS.gene42627:intron 25.0%
! TTGGTTAACCTTAATTGTAT+AGG + chr3.4:91914871-91914890 MS.gene42627:intron 25.0%
!! TGGTCAGAAACATTTTATAT+CGG + chr3.4:91914539-91914558 MS.gene42627:intron 25.0%
!! TTTCTATTTCTCAACTTCTT+AGG + chr3.4:91915048-91915067 MS.gene42627:intron 25.0%
!!! ACCTTGTTATTATAAGCAAT+TGG - chr3.4:91914713-91914732 None:intergenic 25.0%
!!! ATTGGAAATACCGTTTTAAA+TGG - chr3.4:91915130-91915149 None:intergenic 25.0%
AAACAAGTAATTAGTACCTG+TGG - chr3.4:91915031-91915050 None:intergenic 30.0%
ATGAAAGAAGAATGTATGAC+TGG + chr3.4:91914921-91914940 MS.gene42627:CDS 30.0%
ATGTCACTCTATACTTTAAC+AGG + chr3.4:91914685-91914704 MS.gene42627:intron 30.0%
ATTACAGATGCAGATCATTA+TGG + chr3.4:91914481-91914500 MS.gene42627:CDS 30.0%
CTAGCATTTGTGTTATCAAT+TGG + chr3.4:91915221-91915240 MS.gene42627:CDS 30.0%
GCCAATTGCTTATAATAACA+AGG + chr3.4:91914709-91914728 MS.gene42627:CDS 30.0%
TAATAACAAGGTTCGAGAAT+TGG + chr3.4:91914721-91914740 MS.gene42627:CDS 30.0%
TCATACATTCTTCTTTCATC+CGG - chr3.4:91914920-91914939 None:intergenic 30.0%
! AACGGTATTTCCAATTTTCA+TGG + chr3.4:91915135-91915154 MS.gene42627:intron 30.0%
!! TGATTCTTTTGGAAGTTCAA+TGG - chr3.4:91914323-91914342 None:intergenic 30.0%
!!! TTTTTTCTGTAAAACAAGCG+CGG + chr3.4:91914233-91914252 MS.gene42627:CDS 30.0%
ACTTGGAATGCAAATTGTAG+TGG - chr3.4:91914170-91914189 None:intergenic 35.0%
ATAACACAGACACATACGTT+TGG + chr3.4:91914960-91914979 MS.gene42627:CDS 35.0%
TATTATAGGTGAGACTTGAG+AGG + chr3.4:91914841-91914860 MS.gene42627:intron 35.0%
! ATGTTTCTGACCATGTCATA+TGG - chr3.4:91914532-91914551 None:intergenic 35.0%
! CTATTTCTCAACTTCTTAGG+AGG + chr3.4:91915051-91915070 MS.gene42627:intron 35.0%
! GTATTTCCAATTTTCATGGC+AGG + chr3.4:91915139-91915158 MS.gene42627:intron 35.0%
AAGAGGATGTCCATATGACA+TGG + chr3.4:91914519-91914538 MS.gene42627:CDS 40.0%
AATGCAAATTGTAGTGGTGG+TGG - chr3.4:91914164-91914183 None:intergenic 40.0%
ACCGAAACATCAACCTCAAA+TGG - chr3.4:91914986-91915005 None:intergenic 40.0%
ACTGGTTGGATACTTTCTAG+CGG + chr3.4:91915187-91915206 MS.gene42627:CDS 40.0%
ATACACTATCATGTCATCGC+CGG + chr3.4:91914898-91914917 MS.gene42627:CDS 40.0%
ATGTCATATGGACATCCTCT+TGG - chr3.4:91914520-91914539 None:intergenic 40.0%
ATTGGAGAGTCAAGGTTTAG+AGG + chr3.4:91914739-91914758 MS.gene42627:CDS 40.0%
CAAGTAATTAGTACCTGTGG+TGG - chr3.4:91915028-91915047 None:intergenic 40.0%
GATGTTTCGGTACTTCAAGA+AGG + chr3.4:91914995-91915014 MS.gene42627:CDS 40.0%
GCAGATCATTATGGTAGACT+TGG + chr3.4:91914490-91914509 MS.gene42627:CDS 40.0%
TGAAAACTCTGCAGGTTCAA+CGG + chr3.4:91914279-91914298 MS.gene42627:CDS 40.0%
TGGAAAACATACGTATGCCA+CGG - chr3.4:91914384-91914403 None:intergenic 40.0%
TGGAATGCAAATTGTAGTGG+TGG - chr3.4:91914167-91914186 None:intergenic 40.0%
TGTGTCTGTGTTATCTTCTC+TGG - chr3.4:91914954-91914973 None:intergenic 40.0%
!! AACTTCTTAGGAGGTGTTTG+AGG + chr3.4:91915060-91915079 MS.gene42627:intron 40.0%
!! GCCATTTGAGGTTGATGTTT+CGG + chr3.4:91914982-91915001 MS.gene42627:CDS 40.0%
!! TAGAAAGTATCCAACCAGTC+TGG - chr3.4:91915186-91915205 None:intergenic 40.0%
!! TTGCTTGGAGTGTTGTTACT+TGG - chr3.4:91914187-91914206 None:intergenic 40.0%
AACAACACTCCAAGCAAGCT+AGG + chr3.4:91914190-91914209 MS.gene42627:CDS 45.0%
AAGTATCCAACCAGTCTGGT+TGG - chr3.4:91915182-91915201 None:intergenic 45.0%
AGACTTGAGAGGTGGTGATT+TGG + chr3.4:91914852-91914871 MS.gene42627:intron 45.0%
CACATACGTTTGGCCATTTG+AGG + chr3.4:91914970-91914989 MS.gene42627:CDS 45.0%
CAGGTTCCTGCCATGAAAAT+TGG - chr3.4:91915148-91915167 None:intergenic 45.0%
GTAATTAGTACCTGTGGTGG+AGG - chr3.4:91915025-91915044 None:intergenic 45.0%
GTTCGAGAATTGGAGAGTCA+AGG + chr3.4:91914731-91914750 MS.gene42627:CDS 45.0%
TATAGGTGAGACTTGAGAGG+TGG + chr3.4:91914844-91914863 MS.gene42627:intron 45.0%
! AGCTCTAGTGAAAACTCTGC+AGG + chr3.4:91914271-91914290 MS.gene42627:CDS 45.0%
! ATTTTCATGGCAGGAACCTG+AGG + chr3.4:91915148-91915167 MS.gene42627:intron 45.0%
! CTGGTTGGATACTTTCTAGC+GGG + chr3.4:91915188-91915207 MS.gene42627:CDS 45.0%
! TTGGATACTTTCTAGCGGGA+TGG + chr3.4:91915192-91915211 MS.gene42627:CDS 45.0%
!! GTTGAGAATCCTAGCTTGCT+TGG - chr3.4:91914202-91914221 None:intergenic 45.0%
!! TTCTTAGGAGGTGTTTGAGG+TGG + chr3.4:91915063-91915082 MS.gene42627:intron 45.0%
AAACTCTGCAGGTTCAACGG+AGG + chr3.4:91914282-91914301 MS.gene42627:CDS 50.0%
AGGTTCAACGGAGGTTGAGT+CGG + chr3.4:91914291-91914310 MS.gene42627:CDS 50.0%
GGTAGACTTGGCTTACCAAG+AGG + chr3.4:91914502-91914521 MS.gene42627:CDS 50.0%
!! AATTGTAGTGGTGGTGGTGG+AGG - chr3.4:91914158-91914177 None:intergenic 50.0%
!! GCAAATTGTAGTGGTGGTGG+TGG - chr3.4:91914161-91914180 None:intergenic 50.0%
AACGTCGAAAGAGCTCTCCG+TGG + chr3.4:91914364-91914383 MS.gene42627:CDS 55.0%
AGAAGGAGATCCTCCACCAC+AGG + chr3.4:91915012-91915031 MS.gene42627:CDS 55.0%
GGATGTAGGACCAACCAGAC+TGG + chr3.4:91915169-91915188 MS.gene42627:CDS 55.0%
GTAGGACCAACCAGACTGGT+TGG + chr3.4:91915173-91915192 MS.gene42627:CDS 55.0%
TGGCAGGAACCTGAGGATGT+AGG + chr3.4:91915155-91915174 MS.gene42627:intron 55.0%
! CTGGTTGGTCCTACATCCTC+AGG - chr3.4:91915167-91915186 None:intergenic 55.0%
!! AGTGGTGGTGGTGGAGGACA+AGG - chr3.4:91914152-91914171 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 91914145 91915256 91914145 ID=MS.gene42627
chr3.4 mRNA 91914145 91915256 91914145 ID=MS.gene42627.t1;Parent=MS.gene42627
chr3.4 exon 91914145 91914391 91914145 ID=MS.gene42627.t1.exon1;Parent=MS.gene42627.t1
chr3.4 CDS 91914145 91914391 91914145 ID=cds.MS.gene42627.t1;Parent=MS.gene42627.t1
chr3.4 exon 91914479 91914540 91914479 ID=MS.gene42627.t1.exon2;Parent=MS.gene42627.t1
chr3.4 CDS 91914479 91914540 91914479 ID=cds.MS.gene42627.t1;Parent=MS.gene42627.t1
chr3.4 exon 91914707 91914796 91914707 ID=MS.gene42627.t1.exon3;Parent=MS.gene42627.t1
chr3.4 CDS 91914707 91914796 91914707 ID=cds.MS.gene42627.t1;Parent=MS.gene42627.t1
chr3.4 exon 91914893 91915033 91914893 ID=MS.gene42627.t1.exon4;Parent=MS.gene42627.t1
chr3.4 CDS 91914893 91915033 91914893 ID=cds.MS.gene42627.t1;Parent=MS.gene42627.t1
chr3.4 exon 91915161 91915256 91915161 ID=MS.gene42627.t1.exon5;Parent=MS.gene42627.t1
chr3.4 CDS 91915161 91915256 91915161 ID=cds.MS.gene42627.t1;Parent=MS.gene42627.t1
Gene Sequence

>MS.gene42627

ATGGCCTTGTCCTCCACCACCACCACTACAATTTGCATTCCAAGTAACAACACTCCAAGCAAGCTAGGATTCTCAACTAATAACATTGTTTTTTCTGTAAAACAAGCGCGGAAACTATGTATCAGAAGCTCTAGTGAAAACTCTGCAGGTTCAACGGAGGTTGAGTCGGAGAGTTCCATTGAACTTCCAAAAGAATCATCTTCTTTAATTTCTGTTCTCAACGTCGAAAGAGCTCTCCGTGGCATACGTATGTTTTCCATAATGTTGTTGTTATTATATGCTTAAAATTGCATTGCATTGAATTGAATTGATGAAGATATGGTTTAGAATGCAGCTATTACAGATGCAGATCATTATGGTAGACTTGGCTTACCAAGAGGATGTCCATATGACATGGTCAGAAACATTTTATATCGGATTTAATTCATATGAAAGGATTTTTATACAGTCAATCAATCATATTCGTCAAACATGAAAATCATTTGACTTCTAAAGTCACGCATGTATGATTGATGTTAATTCAACTTATCTTTTTGATCTATGTCACTCTATACTTTAACAGGTGCCAATTGCTTATAATAACAAGGTTCGAGAATTGGAGAGTCAAGGTTTAGAGGAAGACATACTTAACAAGAAATTGGAACTTCTCAGAGTTAGTATATATGCATGATTTGAATTGTTGTGTGTTTGCATCATTATTATAGGTGAGACTTGAGAGGTGGTGATTTGGTTAACCTTAATTGTATAGGAATCATACACTATCATGTCATCGCCGGATGAAAGAAGAATGTATGACTGGAGCTTGTCCAGAGAAGATAACACAGACACATACGTTTGGCCATTTGAGGTTGATGTTTCGGTACTTCAAGAAGGAGATCCTCCACCACAGGTACTAATTACTTGTTTCTATTTCTCAACTTCTTAGGAGGTGTTTGAGGTGGAGAGAATTTCTCAAATATGATAATGAATTTGTTGAAATGATCCATTTAAAACGGTATTTCCAATTTTCATGGCAGGAACCTGAGGATGTAGGACCAACCAGACTGGTTGGATACTTTCTAGCGGGATGGATACTGCTAGCATTTGTGTTATCAATTGGACTAAACTTATAG

Protein sequence

>MS.gene42627.t1

MALSSTTTTTICIPSNNTPSKLGFSTNNIVFSVKQARKLCIRSSSENSAGSTEVESESSIELPKESSSLISVLNVERALRGIPITDADHYGRLGLPRGCPYDMVPIAYNNKVRELESQGLEEDILNKKLELLRESYTIMSSPDERRMYDWSLSREDNTDTYVWPFEVDVSVLQEGDPPPQEPEDVGPTRLVGYFLAGWILLAFVLSIGLNL