AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0623330


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0623330 MtrunA17_Chr4g0003001 98.077 208 4 0 1 208 58 265 4.69e-156 432
Msa0623330 MtrunA17_Chr2g0333351 60.000 220 65 10 1 208 1 209 4.15e-76 228
Msa0623330 MtrunA17_Chr4g0064971 48.000 150 67 4 1 149 1 140 5.53e-35 124
Msa0623330 MtrunA17_Chr2g0304551 61.290 93 33 2 1 92 1 91 3.05e-31 114
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0623330 AT1G15760.1 49.545 220 73 10 3 207 5 201 5.17e-59 184
Msa0623330 AT1G80520.1 50.926 216 72 10 1 207 1 191 3.07e-51 164
Msa0623330 AT2G12462.1 30.709 254 123 9 1 207 1 248 6.01e-27 103

Find 44 sgRNAs with CRISPR-Local

Find 44 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCATCTCTTGTATATGAGTA+TGG 0.307188 4_3:+534396 Msa0623330:CDS
CCATGCTTTGGAGAAATTCA+TGG 0.323501 4_3:-534470 None:intergenic
GCTAGTAGTAGTAGTTATTA+TGG 0.324043 4_3:+534693 Msa0623330:CDS
AAACTTGCAAGAAAAGAAAA+AGG 0.339805 4_3:+534528 Msa0623330:CDS
GAGAATTTCTAGCCTGTGTT+TGG 0.345241 4_3:-534505 None:intergenic
CTATGGAGATTCCCATGCTT+TGG 0.347816 4_3:-534482 None:intergenic
ATGATCTTTGCCATTGGTCT+TGG 0.351786 4_3:-534564 None:intergenic
ATCATGATAGCAATGGTTAT+TGG 0.385458 4_3:+534901 Msa0623330:CDS
CAACCATGGATTGGTTCTCT+TGG 0.390859 4_3:+534352 None:intergenic
ATCTTTGCCATTGGTCTTGG+TGG 0.398986 4_3:-534561 None:intergenic
CAGCTGCTGTTGAAGATATC+AGG 0.407684 4_3:+534925 Msa0623330:CDS
TTCTAGCCTGTGTTTGGCTA+TGG 0.411531 4_3:-534499 None:intergenic
GCTCCCATGATCTTTGCCAT+TGG 0.442339 4_3:-534570 None:intergenic
GAGAGGCTGTTGCTTACAAA+TGG 0.465042 4_3:+534780 Msa0623330:CDS
AAGACCAATGGCAAAGATCA+TGG 0.469365 4_3:+534566 Msa0623330:CDS
GCAGGCATCATTGAAGGACT+AGG 0.479985 4_3:-534807 None:intergenic
TTGTTGTAGTTCCAACATCA+AGG 0.486008 4_3:+534670 Msa0623330:CDS
GAAGAGGAATAAGAAGTTGA+AGG 0.494849 4_3:+534746 Msa0623330:CDS
TAACTACATAATGAGAAAGT+TGG 0.500944 4_3:+534620 Msa0623330:CDS
TCAACTTACCAACCATGGAT+TGG 0.503114 4_3:+534343 None:intergenic
GCAAGAGCAGGCATCATTGA+AGG 0.509469 4_3:-534813 None:intergenic
CATATACAAGAGATGGATCA+AGG 0.528896 4_3:-534389 None:intergenic
CTACTACTAGCCCTTGATGT+TGG 0.534119 4_3:-534681 None:intergenic
TTTCATGAAGGAAAGATGAA+AGG 0.538904 4_3:+534873 Msa0623330:CDS
CCATGAATTTCTCCAAAGCA+TGG 0.539592 4_3:+534470 Msa0623330:CDS
CCATACTCATATACAAGAGA+TGG 0.559337 4_3:-534396 None:intergenic
AGACCAATGGCAAAGATCAT+GGG 0.562788 4_3:+534567 Msa0623330:CDS
GAGGAATAAGAAGTTGAAGG+TGG 0.566010 4_3:+534749 Msa0623330:CDS
CATGAATTTCTCCAAAGCAT+GGG 0.566734 4_3:+534471 Msa0623330:CDS
CAGGCATCATTGAAGGACTA+GGG 0.582193 4_3:-534806 None:intergenic
GAGACAACCACCAAGACCAA+TGG 0.584520 4_3:+534554 Msa0623330:CDS
TCATGAAAATGATAAACCAT+AGG 0.596177 4_3:-534858 None:intergenic
GGAAGAGTTCTGTTGTGAAG+AGG 0.606689 4_3:+534730 Msa0623330:CDS
AAACTATCAAGAGCAAGAGC+AGG 0.606845 4_3:-534825 None:intergenic
TGTTGTAGTTCCAACATCAA+GGG 0.608033 4_3:+534671 Msa0623330:CDS
CTGCTGTTGAAGATATCAGG+TGG 0.617099 4_3:+534928 Msa0623330:CDS
GGAGATGATCATGATAGCAA+TGG 0.631701 4_3:+534894 Msa0623330:CDS
GCTCATTGTGTGCAAAAGTG+AGG 0.635769 4_3:-534419 None:intergenic
TGCTGTTGAAGATATCAGGT+GGG 0.636800 4_3:+534929 Msa0623330:CDS
AAGCCAAGAGAACCAATCCA+TGG 0.643677 4_3:-534355 None:intergenic
ATTATGGAACAAGATCATCA+TGG 0.658028 4_3:+534709 Msa0623330:CDS
TGAGCTAGAACAAGAAGACA+TGG 0.668504 4_3:+534437 Msa0623330:CDS
GAATCTCCATAGCCAAACAC+AGG 0.676222 4_3:+534493 Msa0623330:CDS
TGAAGGTGGATAAACAAGAG+AGG 0.697861 4_3:+534763 Msa0623330:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAACTTGCAAGAAAAGAAAA+AGG + chr4_3:534528-534547 Msa0623330:CDS 25.0%
!!! AAAAAGACAAAAAAGTGCTT+AGG + chr4_3:534597-534616 Msa0623330:CDS 25.0%
! TAACTACATAATGAGAAAGT+TGG + chr4_3:534620-534639 Msa0623330:CDS 25.0%
! TCATGAAAATGATAAACCAT+AGG - chr4_3:534861-534880 None:intergenic 25.0%
!! ATGGTTTATCATTTTCATGA+AGG + chr4_3:534861-534880 Msa0623330:CDS 25.0%
GCTAGTAGTAGTAGTTATTA+TGG + chr4_3:534693-534712 Msa0623330:CDS 30.0%
ATTATGGAACAAGATCATCA+TGG + chr4_3:534709-534728 Msa0623330:CDS 30.0%
! TAGTTTTTGTAGTACTCCTA+TGG + chr4_3:534842-534861 Msa0623330:CDS 30.0%
TTTCATGAAGGAAAGATGAA+AGG + chr4_3:534873-534892 Msa0623330:CDS 30.0%
ATCATGATAGCAATGGTTAT+TGG + chr4_3:534901-534920 Msa0623330:CDS 30.0%
CATATACAAGAGATGGATCA+AGG - chr4_3:534392-534411 None:intergenic 35.0%
CCATACTCATATACAAGAGA+TGG - chr4_3:534399-534418 None:intergenic 35.0%
CCATCTCTTGTATATGAGTA+TGG + chr4_3:534396-534415 Msa0623330:CDS 35.0%
CATGAATTTCTCCAAAGCAT+GGG + chr4_3:534471-534490 Msa0623330:CDS 35.0%
TTGTTGTAGTTCCAACATCA+AGG + chr4_3:534670-534689 Msa0623330:CDS 35.0%
TGTTGTAGTTCCAACATCAA+GGG + chr4_3:534671-534690 Msa0623330:CDS 35.0%
GAAGAGGAATAAGAAGTTGA+AGG + chr4_3:534746-534765 Msa0623330:CDS 35.0%
TGAGCTAGAACAAGAAGACA+TGG + chr4_3:534437-534456 Msa0623330:CDS 40.0%
!! CCATGCTTTGGAGAAATTCA+TGG - chr4_3:534473-534492 None:intergenic 40.0%
CCATGAATTTCTCCAAAGCA+TGG + chr4_3:534470-534489 Msa0623330:CDS 40.0%
!! GAGAATTTCTAGCCTGTGTT+TGG - chr4_3:534508-534527 None:intergenic 40.0%
! ATGATCTTTGCCATTGGTCT+TGG - chr4_3:534567-534586 None:intergenic 40.0%
AAGACCAATGGCAAAGATCA+TGG + chr4_3:534566-534585 Msa0623330:CDS 40.0%
AGACCAATGGCAAAGATCAT+GGG + chr4_3:534567-534586 Msa0623330:CDS 40.0%
GAGGAATAAGAAGTTGAAGG+TGG + chr4_3:534749-534768 Msa0623330:CDS 40.0%
TGAAGGTGGATAAACAAGAG+AGG + chr4_3:534763-534782 Msa0623330:CDS 40.0%
AAACTATCAAGAGCAAGAGC+AGG - chr4_3:534828-534847 None:intergenic 40.0%
GGAGATGATCATGATAGCAA+TGG + chr4_3:534894-534913 Msa0623330:CDS 40.0%
TGCTGTTGAAGATATCAGGT+GGG + chr4_3:534929-534948 Msa0623330:CDS 40.0%
GCTCATTGTGTGCAAAAGTG+AGG - chr4_3:534422-534441 None:intergenic 45.0%
CTATGGAGATTCCCATGCTT+TGG - chr4_3:534485-534504 None:intergenic 45.0%
GAATCTCCATAGCCAAACAC+AGG + chr4_3:534493-534512 Msa0623330:CDS 45.0%
TTCTAGCCTGTGTTTGGCTA+TGG - chr4_3:534502-534521 None:intergenic 45.0%
!! ATCTTTGCCATTGGTCTTGG+TGG - chr4_3:534564-534583 None:intergenic 45.0%
CTACTACTAGCCCTTGATGT+TGG - chr4_3:534684-534703 None:intergenic 45.0%
GGAAGAGTTCTGTTGTGAAG+AGG + chr4_3:534730-534749 Msa0623330:CDS 45.0%
! GAGAGGCTGTTGCTTACAAA+TGG + chr4_3:534780-534799 Msa0623330:CDS 45.0%
CAGGCATCATTGAAGGACTA+GGG - chr4_3:534809-534828 None:intergenic 45.0%
CAGCTGCTGTTGAAGATATC+AGG + chr4_3:534925-534944 Msa0623330:CDS 45.0%
CTGCTGTTGAAGATATCAGG+TGG + chr4_3:534928-534947 Msa0623330:CDS 45.0%
GAGACAACCACCAAGACCAA+TGG + chr4_3:534554-534573 Msa0623330:CDS 50.0%
GCTCCCATGATCTTTGCCAT+TGG - chr4_3:534573-534592 None:intergenic 50.0%
GCAGGCATCATTGAAGGACT+AGG - chr4_3:534810-534829 None:intergenic 50.0%
GCAAGAGCAGGCATCATTGA+AGG - chr4_3:534816-534835 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 534357 534983 534357 ID=Msa0623330;Name=Msa0623330
chr4_3 mRNA 534357 534983 534357 ID=Msa0623330-mRNA-1;Parent=Msa0623330;Name=Msa0623330-mRNA-1;_AED=0.23;_eAED=0.23;_QI=0|-1|0|1|-1|1|1|0|208
chr4_3 exon 534357 534983 534357 ID=Msa0623330-mRNA-1:exon:185;Parent=Msa0623330-mRNA-1
chr4_3 CDS 534357 534983 534357 ID=Msa0623330-mRNA-1:cds;Parent=Msa0623330-mRNA-1
Gene Sequence

>Msa0623330

ATGGATTGGTTCTCTTGGCTTTCAAAAACAAACCTTGATCCATCTCTTGTATATGAGTATGGCCTCACTTTTGCACACAATGAGCTAGAACAAGAAGACATGGTTTACTTCAACCATGAATTTCTCCAAAGCATGGGAATCTCCATAGCCAAACACAGGCTAGAAATTCTCAAACTTGCAAGAAAAGAAAAAGGAAAGAGACAACCACCAAGACCAATGGCAAAGATCATGGGAGCAATCAAAAAGACAAAAAAGTGCTTAGGTAACTACATAATGAGAAAGTTGGTTACATGTGAAGAATCAAATTCAGCACTTGTTGTAGTTCCAACATCAAGGGCTAGTAGTAGTAGTTATTATGGAACAAGATCATCATGGAAGAGTTCTGTTGTGAAGAGGAATAAGAAGTTGAAGGTGGATAAACAAGAGAGGCTGTTGCTTACAAATGGAAGCCCTAGTCCTTCAATGATGCCTGCTCTTGCTCTTGATAGTTTTTGTAGTACTCCTATGGTTTATCATTTTCATGAAGGAAAGATGAAAGGAGATGATCATGATAGCAATGGTTATTGGTCAGCTGCTGTTGAAGATATCAGGTGGGATACTATGTTCCAAGATCTAAAACCAAACTAA

Protein sequence

>Msa0623330

MDWFSWLSKTNLDPSLVYEYGLTFAHNELEQEDMVYFNHEFLQSMGISIAKHRLEILKLARKEKGKRQPPRPMAKIMGAIKKTKKCLGNYIMRKLVTCEESNSALVVVPTSRASSSSYYGTRSSWKSSVVKRNKKLKVDKQERLLLTNGSPSPSMMPALALDSFCSTPMVYHFHEGKMKGDDHDSNGYWSAAVEDIRWDTMFQDLKPN*