AlfalfaGEDB | Msa1319650

M. sativa cultivar zhongmu-4 / Msa1319650

KEGG: K08332
GO: GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
PFAM: PF00514.26

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1319650	KEH18966.1	97.751	578	13	0	8	585	7	584	0.0	1142

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1319650	sp\|O22193\|PUB4_ARATH	25.000	324	197	7	172	494	537	815	1.16e-14	81.3

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1319650	A0A072TP55	97.751	578	13	0	8	585	7	584	0.0	1142

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000550	Msa1319650	-0.828629	8.084043e-55	-8.615850e-47
Msa0004920	Msa1319650	0.815018	1.145828e-51	-8.615850e-47
Msa0007080	Msa1319650	0.823709	1.196953e-53	-8.615850e-47
Msa0017510	Msa1319650	0.832323	1.009160e-55	-8.615850e-47
Msa0020930	Msa1319650	0.801132	1.040325e-48	-8.615850e-47
Msa0028640	Msa1319650	0.804038	2.616816e-49	-8.615850e-47
Msa0036430	Msa1319650	0.804582	2.015571e-49	-8.615850e-47
Msa0040450	Msa1319650	0.841751	3.930394e-58	-8.615850e-47
Msa0046100	Msa1319650	0.806606	7.581392e-50	-8.615850e-47
Msa0047050	Msa1319650	0.805588	1.241863e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1319650	MtrunA17_Chr8g0350681	98.039	561	11	0	25	585	1	561	0.0	1103
Msa1319650	MtrunA17_Chr7g0246731	78.369	564	115	3	25	585	1	560	0.0	884
Msa1319650	MtrunA17_Chr3g0131821	35.009	537	323	13	61	584	30	553	1.12e-78	258
Msa1319650	MtrunA17_Chr4g0048401	30.020	503	309	14	49	525	27	512	5.98e-52	186
Msa1319650	MtrunA17_Chr4g0002071	31.707	164	109	3	421	581	13	176	1.13e-20	90.1
Msa1319650	MtrunA17_Chr1g0152561	29.237	236	155	6	262	490	101	331	1.82e-19	90.1
Msa1319650	MtrunA17_Chr1g0183781	28.862	246	162	7	262	502	480	717	1.14e-17	87.0
Msa1319650	MtrunA17_Chr7g0261371	28.099	242	155	9	262	493	554	786	1.99e-15	79.7
Msa1319650	MtrunA17_Chr7g0261371	31.034	203	134	4	361	560	528	727	3.88e-11	66.2
Msa1319650	MtrunA17_Chr7g0248251	27.240	279	194	7	225	498	370	644	1.27e-14	77.0

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1319650	AT2G45720.1	67.201	561	176	4	25	585	1	553	0.0	752
Msa1319650	AT2G45720.2	67.201	561	176	4	25	585	1	553	0.0	752
Msa1319650	AT2G45720.5	67.201	561	176	4	25	585	1	553	0.0	752
Msa1319650	AT2G45720.3	67.201	561	176	4	25	585	1	553	0.0	752
Msa1319650	AT2G45720.4	67.201	561	176	4	25	585	1	553	0.0	752
Msa1319650	AT1G01830.1	62.807	570	205	5	18	584	9	574	0.0	676
Msa1319650	AT1G01830.3	62.807	570	205	5	18	584	9	574	0.0	676
Msa1319650	AT1G01830.2	62.807	570	205	5	18	584	9	574	0.0	676
Msa1319650	AT5G50900.1	38.086	533	310	9	64	584	30	554	5.39e-102	320
Msa1319650	AT1G61350.1	27.971	547	359	12	62	583	32	568	2.52e-61	213

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Find 93 sgRNAs with CRISPR-Local

Find 214 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATACGCCGTTTGTTGCCT+CGG	0.438321	8_4:-23353728	Msa1319650:CDS
AAATCGAAGAGAGGTTAAGA+AGG	0.584354	8_4:-23353813	Msa1319650:CDS
AACTATACTATCTTCCACCA+TGG	0.626237	8_4:+23355236	None:intergenic
AACTGTGGTGTGCAAAACCT+TGG	0.584865	8_4:-23353429	Msa1319650:three_prime_UTR
AAGAAAATGTTAGGTGAAGT+TGG	0.452479	8_4:-23353932	Msa1319650:CDS
AAGAGAAACCAGAGTTTCCT+CGG	0.556662	8_4:+23354057	None:intergenic
AAGAGGTTGATGATTTCATA+TGG	0.439272	8_4:-23353680	Msa1319650:CDS
AAGTGATCCGAGGCAACAAA+CGG	0.527449	8_4:+23353721	None:intergenic
AATCTGCTGTTGGTGCATTG+AGG	0.654433	8_4:-23354099	Msa1319650:CDS
ACAAAAGCAAGAGAGGTAAA+AGG	0.285811	8_4:-23355141	Msa1319650:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTGAGAATCAAACACCAAAA+GGG	+	chr8_4:23353396-23353415	None:intergenic	30.0%
ATTGAGAATCAAACACCAAA+AGG	+	chr8_4:23353397-23353416	None:intergenic	30.0%
TCATCAATCAAAAATACAGC+TGG	+	chr8_4:23353478-23353497	None:intergenic	30.0%
TCTTAGTTGCTCATTCTTAA+AGG	-	chr8_4:23353645-23353664	Msa1319650:CDS	30.0%
GATATGATATCAAATGGTCA+AGG	+	chr8_4:23354257-23354276	None:intergenic	30.0%
GTAATTGAAGCAAATCTAGA+TGG	+	chr8_4:23354317-23354336	None:intergenic	30.0%
AGTTAATAATGTATGTGAGG+TGG	-	chr8_4:23354378-23354397	Msa1319650:CDS	30.0%
ATGGCTGTTAATGAAAATGA+AGG	+	chr8_4:23354513-23354532	None:intergenic	30.0%
AGTGATCATTTAATACCCTT+TGG	+	chr8_4:23354594-23354613	None:intergenic	30.0%
AAGTAAGAGAAATACTCATG+GGG	-	chr8_4:23354619-23354638	Msa1319650:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_4	gene	23353357	23357190	23353357	ID=Msa1319650;Name=Msa1319650
chr8_4	mRNA	23353357	23357190	23353357	ID=Msa1319650-mRNA-1;Parent=Msa1319650;Name=Msa1319650-mRNA-1;_AED=0.10;_eAED=0.11;_QI=0\|0\|0.25\|1\|0\|0\|4\|214\|585
chr8_4	exon	23353357	23355308	23353357	ID=Msa1319650-mRNA-1:exon:8135;Parent=Msa1319650-mRNA-1
chr8_4	exon	23356649	23356656	23356649	ID=Msa1319650-mRNA-1:exon:8134;Parent=Msa1319650-mRNA-1
chr8_4	exon	23357171	23357178	23357171	ID=Msa1319650-mRNA-1:exon:8133;Parent=Msa1319650-mRNA-1
chr8_4	exon	23357187	23357190	23357187	ID=Msa1319650-mRNA-1:exon:8132;Parent=Msa1319650-mRNA-1
chr8_4	CDS	23357187	23357190	23357187	ID=Msa1319650-mRNA-1:cds;Parent=Msa1319650-mRNA-1
chr8_4	CDS	23357171	23357178	23357171	ID=Msa1319650-mRNA-1:cds;Parent=Msa1319650-mRNA-1
chr8_4	CDS	23356649	23356656	23356649	ID=Msa1319650-mRNA-1:cds;Parent=Msa1319650-mRNA-1
chr8_4	CDS	23353571	23355308	23353571	ID=Msa1319650-mRNA-1:cds;Parent=Msa1319650-mRNA-1
chr8_4	three_prime_UTR	23353357	23353570	23353357	ID=Msa1319650-mRNA-1:three_prime_utr;Parent=Msa1319650-mRNA-1

Gene Sequence

>Msa1319650

ATGGTAAATATTCAAATCAACTTTGGTGATCATCATCATTTCTACAACACTACTACTACAAATTTTCAAACCATGGTGGAAGATAGTATAGTTGCTGTCAAAGTTGATACATGTTCAACAGAAGAATGGTTGTTGAACTCACAAGAACTTGTTCCTATTGCTCTTACAAAAGCAAGAGAGGTAAAAGGGTTTCAAGGTAGGTGGAAAATGATCATTTCAAAATTGGAACAAGTTCCATCAAAGCTATCAGATTTATCAAGCCACCCTTGTTTTTCAAAGAATGCACTTTGCAAGGAACAATTGCAGGCTGTGTCAAAGACACTAAGAGAAGGTATTGAATTAGCTGAGTTATGTTTGAAAGAAAAGTATGAAGGAAAACTAAGAATGCAAAGTGATCTTGATGCATTAATTGGTAAGCTTGATTTGAATTTAAAGGATTGTAGCTTGTTAACAAAAACTGGTGTACTTGGTGAAGTTACTTTGCAATTCAATGTTTCTGGTTCTATAACAGAATCAGATATTGAAACACATTGCAATATAAAGGAGCTACTCGCGCGCCTTCAAATCGGTCATTTGGAATCGAAACACAAAGCTTTGGATAGACTTCATGATATTATGAGGGAAGATGAGAAGAATGTTTTGGCAGTTTTTTGCAGGAGCAATGTTGTTGCTTTGGTTCAGTTGCTTACAGCAACATCAACAAGGATAAGGGAGAAAACAGTTAGTATTATATGCTCGCTCGTTGAATCCGGTGGTAGCTGCGAAAACTGGTTAGTTTCTGAAGGTGTTTTGCCACCACTTATAAGGCTTGTTGAATCAGGTAGTGCTGTTGCTAAAGAAAAGGCTATTGTTTCTCTTCAAAGGTTGTCTATGACGGAAGACACAACACGTGCGATCGTCGGACATGGAGGGGTTCGTCCGTTAGTCGAGCTTTGTCAGGTTGGTGATTCTGTTTCACAATCTGCAGCTGCTTGTACTTTGAAGAATATTTCGGCTGTTCCGGAAGTGAGACAGGTTTTGGCTGAAGAAGGCGTTGTTAAGGTTATGATCAATCTTCTTAACAATGGAATGTTGTTAGGAACTAAAGAGTATGCTGCTGAGTGTTTGCAGAATCTGACTGCAAGCAATGAGAATCTGAGAAGGTCTGTTATATCAGAAGGCGGCGTTCGAAGCATTTTGGCTTATCTTGATGGTCCACTTCCTCAAGAATCTGCTGTTGGTGCATTGAGGAATCTCGTTGCATCGGTTTCCGAGGAAACTCTGGTTTCTCTTGGATTGCTTCCTTGTTTAGTTCATGTTTTGAAATCAGGATCATTAGGTGCGAAACAATGCGCTGCATCGGTTATATGCAGGATTTGTAGCTCAATTGAGATGAAGAAAATGTTAGGTGAAGTTGGTTGCATTCCTCTATTGATCAACATGCTTGAGGGTAAAGCAAACACTGCAAGAGAACTTGCAGCACAAGCAATTGCAAGCTTGATGATTCTGTCGCAAAATCGAAGAGAGGTTAAGAAGGATGATAAAAGTGTGCCGAATTTGGTTCAATTGCTTGATCCTAGTCCACAAAACACTGCGAAAAAATACGCCGTTTGTTGCCTCGGATCACTTTCTTCTTGTAAGAAATGTAAGAGGTTGATGATTTCATATGGAGCAATAGGGTATTTGAAGAAGCTTATTGAGATGGAGATTCCAGGAGCTAAAAAGTTGCTTGAAAGATTGGAAAGAGGGAAATTGAGAAGCTTGTTTAGCAGAAAATAA

Protein sequence

>Msa1319650

MVNIQINFGDHHHFYNTTTTNFQTMVEDSIVAVKVDTCSTEEWLLNSQELVPIALTKAREVKGFQGRWKMIISKLEQVPSKLSDLSSHPCFSKNALCKEQLQAVSKTLREGIELAELCLKEKYEGKLRMQSDLDALIGKLDLNLKDCSLLTKTGVLGEVTLQFNVSGSITESDIETHCNIKELLARLQIGHLESKHKALDRLHDIMREDEKNVLAVFCRSNVVALVQLLTATSTRIREKTVSIICSLVESGGSCENWLVSEGVLPPLIRLVESGSAVAKEKAIVSLQRLSMTEDTTRAIVGHGGVRPLVELCQVGDSVSQSAAACTLKNISAVPEVRQVLAEEGVVKVMINLLNNGMLLGTKEYAAECLQNLTASNENLRRSVISEGGVRSILAYLDGPLPQESAVGALRNLVASVSEETLVSLGLLPCLVHVLKSGSLGAKQCAASVICRICSSIEMKKMLGEVGCIPLLINMLEGKANTARELAAQAIASLMILSQNRREVKKDDKSVPNLVQLLDPSPQNTAKKYAVCCLGSLSSCKKCKRLMISYGAIGYLKKLIEMEIPGAKKLLERLERGKLRSLFSRK*