AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0510750


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0510750 MtrunA17_Chr3g0116971 96.330 109 3 1 1 108 1 109 3.12e-71 207
Msa0510750 MtrunA17_Chr3g0116951 68.908 119 23 6 1 108 6 121 6.17e-42 135
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 46 sgRNAs with CRISPR-Local

Find 46 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAACCCTCTCAACGATTTC+AGG 0.158944 3_4:+59883975 None:intergenic
TTGAAGGCCATCTCACCATT+TGG 0.332611 3_4:+59884062 None:intergenic
GGTTGTGGTGGTGGCGGCCA+TGG 0.367830 3_4:+59883997 None:intergenic
CCTTCGTGGGTAATGTTGAT+TGG 0.388716 3_4:+59883872 None:intergenic
CGATTTCAGGGTTGTGGTGG+TGG 0.397034 3_4:+59883988 None:intergenic
TGAAGGCCATCTCACCATTT+GGG 0.418823 3_4:+59884063 None:intergenic
CAACCCTCTCAACGATTTCA+GGG 0.425008 3_4:+59883976 None:intergenic
CCATTTGACCTGTTCCTTCG+TGG 0.432698 3_4:+59883858 None:intergenic
ATAGACAACCCTCTTGTTGA+AGG 0.438765 3_4:+59884046 None:intergenic
GTCACTCATGGAATACATTC+AGG 0.444691 3_4:-59883836 Msa0510750:CDS
CATTTGACCTGTTCCTTCGT+GGG 0.467646 3_4:+59883859 None:intergenic
CAACAAGAGGGTTGTCTATG+AGG 0.468986 3_4:-59884042 Msa0510750:CDS
CCCTTCGAACTTCTGAAATG+TGG 0.470828 3_4:+59884108 None:intergenic
TCTCAACGATTTCAGGGTTG+TGG 0.485866 3_4:+59883982 None:intergenic
TTTCAGGGTTGTGGTGGTGG+CGG 0.488542 3_4:+59883991 None:intergenic
CGTTGAGAGGGTTGAAGTGG+TGG 0.489360 3_4:-59883967 Msa0510750:CDS
AACATTACCCACGAAGGAAC+AGG 0.491085 3_4:-59883866 Msa0510750:CDS
TATGAGGAGGATGCGATTGA+AGG 0.492172 3_4:-59884026 Msa0510750:CDS
CCACGAAGGAACAGGTCAAA+TGG 0.498880 3_4:-59883858 Msa0510750:CDS
GTACCTGAGGTTAGGCCCTA+TGG 0.503566 3_4:-59883933 Msa0510750:CDS
CCACCATAGGGCCTAACCTC+AGG 0.504816 3_4:+59883930 None:intergenic
GAATACTCTCGCTACCCAAA+TGG 0.533741 3_4:-59884077 Msa0510750:CDS
CCTTCGAACTTCTGAAATGT+GGG 0.541962 3_4:+59884109 None:intergenic
ATGAACGTGTACCTGAGGTT+AGG 0.542173 3_4:-59883941 Msa0510750:CDS
CCCACATTTCAGAAGTTCGA+AGG 0.543003 3_4:-59884109 Msa0510750:CDS
ACAGGTCAAATGGTCACTCA+TGG 0.552434 3_4:-59883848 Msa0510750:CDS
CAACGATTTCAGGGTTGTGG+TGG 0.564097 3_4:+59883985 None:intergenic
GTAGATCACTTGACCACCAT+AGG 0.569439 3_4:+59883917 None:intergenic
AATGTGGGTGTGGTAGTCAT+TGG 0.581040 3_4:+59884124 None:intergenic
GGGTAGCGAGAGTATTCGCT+AGG 0.581864 3_4:+59884083 None:intergenic
AGTGATCTACGAAGAGGACG+TGG 0.585316 3_4:-59883904 Msa0510750:CDS
TCGCTACCCAAATGGTGAGA+TGG 0.585879 3_4:-59884069 Msa0510750:CDS
GTGAGATGGCCTTCAACAAG+AGG 0.602330 3_4:-59884055 Msa0510750:CDS
TAGATCACTTGACCACCATA+GGG 0.606041 3_4:+59883918 None:intergenic
GAACTTCTGAAATGTGGGTG+TGG 0.614497 3_4:+59884114 None:intergenic
CCACATTTCAGAAGTTCGAA+GGG 0.620439 3_4:-59884108 Msa0510750:CDS
CAACCCTGAAATCGTTGAGA+GGG 0.621410 3_4:-59883979 Msa0510750:CDS
CCTGAGGTTAGGCCCTATGG+TGG 0.626513 3_4:-59883930 Msa0510750:CDS
ACAACCCTGAAATCGTTGAG+AGG 0.647655 3_4:-59883980 Msa0510750:CDS
CAAGAGGGTTGTCTATGAGG+AGG 0.649483 3_4:-59884039 Msa0510750:CDS
GCGATTGAAGGATCACGCCA+TGG 0.655981 3_4:-59884014 Msa0510750:CDS
TGAGATGGCCTTCAACAAGA+GGG 0.673455 3_4:-59884054 Msa0510750:CDS
TGGTCAAGTGATCTACGAAG+AGG 0.694239 3_4:-59883910 Msa0510750:CDS
AATCGTTGAGAGGGTTGAAG+TGG 0.698905 3_4:-59883970 Msa0510750:CDS
CCAATCAACATTACCCACGA+AGG 0.702298 3_4:-59883872 Msa0510750:CDS
GGAATATGAACGTGTACCTG+AGG 0.717370 3_4:-59883946 Msa0510750:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CCTTCGAACTTCTGAAATGT+GGG + chr3_4:59883858-59883877 None:intergenic 40.0%
CCACATTTCAGAAGTTCGAA+GGG - chr3_4:59883856-59883875 Msa0510750:CDS 40.0%
ATAGACAACCCTCTTGTTGA+AGG + chr3_4:59883921-59883940 None:intergenic 40.0%
TAGATCACTTGACCACCATA+GGG + chr3_4:59884049-59884068 None:intergenic 40.0%
GTCACTCATGGAATACATTC+AGG - chr3_4:59884128-59884147 Msa0510750:CDS 40.0%
AATGTGGGTGTGGTAGTCAT+TGG + chr3_4:59883843-59883862 None:intergenic 45.0%
!! GAACTTCTGAAATGTGGGTG+TGG + chr3_4:59883853-59883872 None:intergenic 45.0%
CCCACATTTCAGAAGTTCGA+AGG - chr3_4:59883855-59883874 Msa0510750:CDS 45.0%
!! CCCTTCGAACTTCTGAAATG+TGG + chr3_4:59883859-59883878 None:intergenic 45.0%
GAATACTCTCGCTACCCAAA+TGG - chr3_4:59883887-59883906 Msa0510750:CDS 45.0%
TGAAGGCCATCTCACCATTT+GGG + chr3_4:59883904-59883923 None:intergenic 45.0%
TTGAAGGCCATCTCACCATT+TGG + chr3_4:59883905-59883924 None:intergenic 45.0%
TGAGATGGCCTTCAACAAGA+GGG - chr3_4:59883910-59883929 Msa0510750:CDS 45.0%
CAACAAGAGGGTTGTCTATG+AGG - chr3_4:59883922-59883941 Msa0510750:CDS 45.0%
TATGAGGAGGATGCGATTGA+AGG - chr3_4:59883938-59883957 Msa0510750:CDS 45.0%
TCTCAACGATTTCAGGGTTG+TGG + chr3_4:59883985-59884004 None:intergenic 45.0%
ACAACCCTGAAATCGTTGAG+AGG - chr3_4:59883984-59884003 Msa0510750:CDS 45.0%
CAACCCTGAAATCGTTGAGA+GGG - chr3_4:59883985-59884004 Msa0510750:CDS 45.0%
CAACCCTCTCAACGATTTCA+GGG + chr3_4:59883991-59884010 None:intergenic 45.0%
TCAACCCTCTCAACGATTTC+AGG + chr3_4:59883992-59884011 None:intergenic 45.0%
AATCGTTGAGAGGGTTGAAG+TGG - chr3_4:59883994-59884013 Msa0510750:CDS 45.0%
GGAATATGAACGTGTACCTG+AGG - chr3_4:59884018-59884037 Msa0510750:CDS 45.0%
ATGAACGTGTACCTGAGGTT+AGG - chr3_4:59884023-59884042 Msa0510750:CDS 45.0%
GTAGATCACTTGACCACCAT+AGG + chr3_4:59884050-59884069 None:intergenic 45.0%
TGGTCAAGTGATCTACGAAG+AGG - chr3_4:59884054-59884073 Msa0510750:CDS 45.0%
CCTTCGTGGGTAATGTTGAT+TGG + chr3_4:59884095-59884114 None:intergenic 45.0%
CCAATCAACATTACCCACGA+AGG - chr3_4:59884092-59884111 Msa0510750:CDS 45.0%
AACATTACCCACGAAGGAAC+AGG - chr3_4:59884098-59884117 Msa0510750:CDS 45.0%
CATTTGACCTGTTCCTTCGT+GGG + chr3_4:59884108-59884127 None:intergenic 45.0%
ACAGGTCAAATGGTCACTCA+TGG - chr3_4:59884116-59884135 Msa0510750:CDS 45.0%
TCGCTACCCAAATGGTGAGA+TGG - chr3_4:59883895-59883914 Msa0510750:CDS 50.0%
GTGAGATGGCCTTCAACAAG+AGG - chr3_4:59883909-59883928 Msa0510750:CDS 50.0%
CAAGAGGGTTGTCTATGAGG+AGG - chr3_4:59883925-59883944 Msa0510750:CDS 50.0%
CAACGATTTCAGGGTTGTGG+TGG + chr3_4:59883982-59884001 None:intergenic 50.0%
AGTGATCTACGAAGAGGACG+TGG - chr3_4:59884060-59884079 Msa0510750:CDS 50.0%
CCATTTGACCTGTTCCTTCG+TGG + chr3_4:59884109-59884128 None:intergenic 50.0%
CCACGAAGGAACAGGTCAAA+TGG - chr3_4:59884106-59884125 Msa0510750:CDS 50.0%
!! GGGTAGCGAGAGTATTCGCT+AGG + chr3_4:59883884-59883903 None:intergenic 55.0%
GCGATTGAAGGATCACGCCA+TGG - chr3_4:59883950-59883969 Msa0510750:CDS 55.0%
!! TTTCAGGGTTGTGGTGGTGG+CGG + chr3_4:59883976-59883995 None:intergenic 55.0%
CGATTTCAGGGTTGTGGTGG+TGG + chr3_4:59883979-59883998 None:intergenic 55.0%
CGTTGAGAGGGTTGAAGTGG+TGG - chr3_4:59883997-59884016 Msa0510750:CDS 55.0%
GTACCTGAGGTTAGGCCCTA+TGG - chr3_4:59884031-59884050 Msa0510750:CDS 55.0%
CCACCATAGGGCCTAACCTC+AGG + chr3_4:59884037-59884056 None:intergenic 60.0%
CCTGAGGTTAGGCCCTATGG+TGG - chr3_4:59884034-59884053 Msa0510750:CDS 60.0%
!! GGTTGTGGTGGTGGCGGCCA+TGG + chr3_4:59883970-59883989 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 59883830 59884156 59883830 ID=Msa0510750;Name=Msa0510750
chr3_4 mRNA 59883830 59884156 59883830 ID=Msa0510750-mRNA-1;Parent=Msa0510750;Name=Msa0510750-mRNA-1;_AED=0.27;_eAED=0.27;_QI=0|-1|0|1|-1|1|1|0|108
chr3_4 exon 59883830 59884156 59883830 ID=Msa0510750-mRNA-1:exon:14210;Parent=Msa0510750-mRNA-1
chr3_4 CDS 59883830 59884156 59883830 ID=Msa0510750-mRNA-1:cds;Parent=Msa0510750-mRNA-1
Gene Sequence

>Msa0510750

ATGATGAGATCCAATGACTACCACACCCACATTTCAGAAGTTCGAAGGGAGCCTAGCGAATACTCTCGCTACCCAAATGGTGAGATGGCCTTCAACAAGAGGGTTGTCTATGAGGAGGATGCGATTGAAGGATCACGCCATGGCCGCCACCACCACAACCCTGAAATCGTTGAGAGGGTTGAAGTGGTGGAATATGAACGTGTACCTGAGGTTAGGCCCTATGGTGGTCAAGTGATCTACGAAGAGGACGTGGATGTTGAGACCAATCAACATTACCCACGAAGGAACAGGTCAAATGGTCACTCATGGAATACATTCAGGCCATAA

Protein sequence

>Msa0510750

MMRSNDYHTHISEVRREPSEYSRYPNGEMAFNKRVVYEEDAIEGSRHGRHHHNPEIVERVEVVEYERVPEVRPYGGQVIYEEDVDVETNQHYPRRNRSNGHSWNTFRP*