AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0163670


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0163670 MtrunA17_Chr1g0178131 78.947 95 20 0 1 95 1 95 3.25e-48 158
Msa0163670 MtrunA17_Chr7g0266771 67.073 82 27 0 1 82 2 83 2.40e-32 117
Msa0163670 MtrunA17_Chr8g0344291 51.250 80 38 1 16 94 4 83 3.03e-21 86.7
Msa0163670 MtrunA17_Chr2g0321721 47.436 78 41 0 17 94 6 83 3.14e-20 84.7
Msa0163670 MtrunA17_Chr3g0106991 50.602 83 38 2 15 94 5 87 5.78e-18 78.2
Msa0163670 MtrunA17_Chr3g0106961 48.810 84 40 2 14 94 4 87 1.14e-17 76.3
Msa0163670 MtrunA17_Chr4g0026081 52.632 76 36 0 19 94 24 99 7.58e-17 75.9
Msa0163670 MtrunA17_Chr3g0106971 46.753 77 39 2 15 89 5 81 6.87e-14 66.6
Msa0163670 MtrunA17_Chr3g0106981 46.053 76 39 2 16 89 6 81 7.74e-14 66.6
Msa0163670 MtrunA17_Chr8g0344901 43.023 86 45 3 16 97 4 89 1.36e-13 66.2
Msa0163670 MtrunA17_Chr1g0185631 42.500 80 44 2 11 90 4 81 3.07e-12 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0163670 AT3G44326.1 55.844 77 34 0 16 92 21 97 1.87e-24 97.1
Msa0163670 AT2G27310.1 57.143 77 33 0 15 91 11 87 2.70e-24 96.3
Msa0163670 AT2G36090.1 50.617 81 28 1 13 93 14 82 1.20e-16 75.5

Find 54 sgRNAs with CRISPR-Local

Find 54 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTATCATGATTTGCATTTC+CGG 0.272726 1_4:+41991368 None:intergenic
TCTGTTCCGACGAACATCTC+TGG 0.313553 1_4:+41991522 Msa0163670:CDS
TGGAGGTGGTGCAGCGGAGT+GGG 0.320619 1_4:+41991739 Msa0163670:CDS
CAGACTCTATCTCTTCTCAT+AGG 0.359020 1_4:+41991639 Msa0163670:CDS
CTGTTTGCAACGGTGGTTTA+CGG 0.378960 1_4:+41991774 Msa0163670:CDS
GTGGAGGTGGTGCAGCGGAG+TGG 0.384568 1_4:+41991738 Msa0163670:CDS
GGAGGTGGTGCAGCGGAGTG+GGG 0.386877 1_4:+41991740 Msa0163670:CDS
GACTGGCAAGGCGGTGGAGG+TGG 0.421907 1_4:+41991725 Msa0163670:CDS
CGGAACAGAGAGAGTTGAGT+TGG 0.450504 1_4:-41991508 None:intergenic
CACATACTCACACGCCTTGA+CGG 0.456412 1_4:+41991452 Msa0163670:CDS
GGGAGGAGTTGAGGCTGAGC+TGG 0.460888 1_4:+41991661 Msa0163670:CDS
GTCGACTGGCAAGGCGGTGG+AGG 0.477132 1_4:+41991722 Msa0163670:CDS
GAGAGAGTTGAGTTGGGAGC+AGG 0.498381 1_4:-41991501 None:intergenic
AGACTCTATCTCTTCTCATA+GGG 0.500015 1_4:+41991640 Msa0163670:CDS
AAGCGAGAGCAGCGCCGTCA+AGG 0.502843 1_4:-41991466 None:intergenic
CGGAGAACGTTGAGATTACA+TGG 0.505020 1_4:-41991595 None:intergenic
TTCTCATAGGGGAGGAGTTG+AGG 0.505954 1_4:+41991652 Msa0163670:CDS
GGTGGTGCAGCGGAGTGGGG+AGG 0.507878 1_4:+41991743 Msa0163670:CDS
CGAGCGGTGAACGTGTCGAC+TGG 0.509912 1_4:+41991708 Msa0163670:CDS
TTCAGCCTCCTCTGATTCGC+CGG 0.511615 1_4:-41991387 None:intergenic
GGAACAGAGAGAGTTGAGTT+GGG 0.512955 1_4:-41991507 None:intergenic
CTGTTCCGACGAACATCTCT+GGG 0.515288 1_4:+41991523 Msa0163670:CDS
TTGCATTATGTCGTCGTGGA+CGG 0.534628 1_4:-41991426 None:intergenic
AACAGCGACGGCGACGGAGG+TGG 0.537963 1_4:+41991800 Msa0163670:CDS
TTCACCGCTCGTTGACTCAC+CGG 0.540056 1_4:-41991696 None:intergenic
AGGCGGTGGAGGTGGTGCAG+CGG 0.545534 1_4:+41991733 Msa0163670:CDS
CGTGTCGACTGGCAAGGCGG+TGG 0.547797 1_4:+41991719 Msa0163670:CDS
GCGAACACGTGGCGTGTTGG+TGG 0.547964 1_4:-41991573 None:intergenic
TTGCATTTCCGGCGAATCAG+AGG 0.552766 1_4:+41991379 Msa0163670:CDS
GAAGAAAGAGCGGGAGCCAT+CGG 0.558224 1_4:-41991615 None:intergenic
ACACGTGGCGTGTTGGTGGA+AGG 0.562808 1_4:-41991569 None:intergenic
GAGTTGGGAGCAGGTGCTTG+CGG 0.574852 1_4:-41991492 None:intergenic
CATTTCCGGCGAATCAGAGG+AGG 0.576009 1_4:+41991382 Msa0163670:CDS
GTCGTGGACGGCGGAGAATG+TGG 0.582153 1_4:-41991414 None:intergenic
AGAGTCTGCGAAGAAAGAGC+GGG 0.584331 1_4:-41991624 None:intergenic
CGGAGAAACAGCGACGGCGA+CGG 0.589608 1_4:+41991794 Msa0163670:CDS
TTGGGAGCAGGTGCTTGCGG+CGG 0.592620 1_4:-41991489 None:intergenic
AGAAACAGCGACGGCGACGG+AGG 0.596172 1_4:+41991797 Msa0163670:CDS
GGTGAAGTTGCTGTTTGCAA+CGG 0.596822 1_4:+41991764 Msa0163670:CDS
GGTGAACGTGTCGACTGGCA+AGG 0.597397 1_4:+41991713 Msa0163670:CDS
TAGAGTCTGCGAAGAAAGAG+CGG 0.597944 1_4:-41991625 None:intergenic
GTAATCTCAACGTTCTCCGA+TGG 0.609849 1_4:+41991599 Msa0163670:CDS
ATGGCGAACACGTGGCGTGT+TGG 0.612938 1_4:-41991576 None:intergenic
CATTATGTCGTCGTGGACGG+CGG 0.616775 1_4:-41991423 None:intergenic
GAAGTTGCTGTTTGCAACGG+TGG 0.616823 1_4:+41991767 Msa0163670:CDS
TCTATCTCTTCTCATAGGGG+AGG 0.647985 1_4:+41991644 Msa0163670:CDS
GTTTGCCCAGAGATGTTCGT+CGG 0.658971 1_4:-41991528 None:intergenic
GACTCTATCTCTTCTCATAG+GGG 0.659257 1_4:+41991641 Msa0163670:CDS
GAGATTACATGGCGAACACG+TGG 0.665268 1_4:-41991584 None:intergenic
CAAACGCATGCTATTCTACG+TGG 0.694571 1_4:+41991546 Msa0163670:CDS
GAGTTTGCATTATGTCGTCG+TGG 0.722370 1_4:-41991430 None:intergenic
CGATCCGGTGAGTCAACGAG+CGG 0.722539 1_4:+41991692 Msa0163670:CDS
GGTTTACGGAGAAACAGCGA+CGG 0.737765 1_4:+41991788 Msa0163670:CDS
GAACGTGTCGACTGGCAAGG+CGG 0.744897 1_4:+41991716 Msa0163670:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AGACTCTATCTCTTCTCATA+GGG + chr1_4:41991640-41991659 Msa0163670:CDS 35.0%
CAGACTCTATCTCTTCTCAT+AGG + chr1_4:41991639-41991658 Msa0163670:CDS 40.0%
GACTCTATCTCTTCTCATAG+GGG + chr1_4:41991641-41991660 Msa0163670:CDS 40.0%
TTGCATTATGTCGTCGTGGA+CGG - chr1_4:41991429-41991448 None:intergenic 45.0%
GAGTTTGCATTATGTCGTCG+TGG - chr1_4:41991433-41991452 None:intergenic 45.0%
GGAACAGAGAGAGTTGAGTT+GGG - chr1_4:41991510-41991529 None:intergenic 45.0%
CAAACGCATGCTATTCTACG+TGG + chr1_4:41991546-41991565 Msa0163670:CDS 45.0%
CGGAGAACGTTGAGATTACA+TGG - chr1_4:41991598-41991617 None:intergenic 45.0%
GTAATCTCAACGTTCTCCGA+TGG + chr1_4:41991599-41991618 Msa0163670:CDS 45.0%
TAGAGTCTGCGAAGAAAGAG+CGG - chr1_4:41991628-41991647 None:intergenic 45.0%
TCTATCTCTTCTCATAGGGG+AGG + chr1_4:41991644-41991663 Msa0163670:CDS 45.0%
!! GAGCTGGATTTTGATCGATC+CGG + chr1_4:41991677-41991696 Msa0163670:CDS 45.0%
!! GGTGAAGTTGCTGTTTGCAA+CGG + chr1_4:41991764-41991783 Msa0163670:CDS 45.0%
CTGTTTGCAACGGTGGTTTA+CGG + chr1_4:41991774-41991793 Msa0163670:CDS 45.0%
TTGCATTTCCGGCGAATCAG+AGG + chr1_4:41991379-41991398 Msa0163670:CDS 50.0%
CACATACTCACACGCCTTGA+CGG + chr1_4:41991452-41991471 Msa0163670:CDS 50.0%
CGGAACAGAGAGAGTTGAGT+TGG - chr1_4:41991511-41991530 None:intergenic 50.0%
TCTGTTCCGACGAACATCTC+TGG + chr1_4:41991522-41991541 Msa0163670:CDS 50.0%
CTGTTCCGACGAACATCTCT+GGG + chr1_4:41991523-41991542 Msa0163670:CDS 50.0%
GTTTGCCCAGAGATGTTCGT+CGG - chr1_4:41991531-41991550 None:intergenic 50.0%
GAGATTACATGGCGAACACG+TGG - chr1_4:41991587-41991606 None:intergenic 50.0%
AGAGTCTGCGAAGAAAGAGC+GGG - chr1_4:41991627-41991646 None:intergenic 50.0%
TTCTCATAGGGGAGGAGTTG+AGG + chr1_4:41991652-41991671 Msa0163670:CDS 50.0%
!! GAAGTTGCTGTTTGCAACGG+TGG + chr1_4:41991767-41991786 Msa0163670:CDS 50.0%
GGTTTACGGAGAAACAGCGA+CGG + chr1_4:41991788-41991807 Msa0163670:CDS 50.0%
CATTTCCGGCGAATCAGAGG+AGG + chr1_4:41991382-41991401 Msa0163670:CDS 55.0%
! TTCAGCCTCCTCTGATTCGC+CGG - chr1_4:41991390-41991409 None:intergenic 55.0%
CATTATGTCGTCGTGGACGG+CGG - chr1_4:41991426-41991445 None:intergenic 55.0%
!! GAGAGAGTTGAGTTGGGAGC+AGG - chr1_4:41991504-41991523 None:intergenic 55.0%
GAAGAAAGAGCGGGAGCCAT+CGG - chr1_4:41991618-41991637 None:intergenic 55.0%
!! TTCACCGCTCGTTGACTCAC+CGG - chr1_4:41991699-41991718 None:intergenic 55.0%
GAGTTGGGAGCAGGTGCTTG+CGG - chr1_4:41991495-41991514 None:intergenic 60.0%
! ACACGTGGCGTGTTGGTGGA+AGG - chr1_4:41991572-41991591 None:intergenic 60.0%
ATGGCGAACACGTGGCGTGT+TGG - chr1_4:41991579-41991598 None:intergenic 60.0%
CGATCCGGTGAGTCAACGAG+CGG + chr1_4:41991692-41991711 Msa0163670:CDS 60.0%
!! GGTGAACGTGTCGACTGGCA+AGG + chr1_4:41991713-41991732 Msa0163670:CDS 60.0%
!! GAACGTGTCGACTGGCAAGG+CGG + chr1_4:41991716-41991735 Msa0163670:CDS 60.0%
GTCGTGGACGGCGGAGAATG+TGG - chr1_4:41991417-41991436 None:intergenic 65.0%
AAGCGAGAGCAGCGCCGTCA+AGG - chr1_4:41991469-41991488 None:intergenic 65.0%
TTGGGAGCAGGTGCTTGCGG+CGG - chr1_4:41991492-41991511 None:intergenic 65.0%
GCGAACACGTGGCGTGTTGG+TGG - chr1_4:41991576-41991595 None:intergenic 65.0%
GGGAGGAGTTGAGGCTGAGC+TGG + chr1_4:41991661-41991680 Msa0163670:CDS 65.0%
! CGAGCGGTGAACGTGTCGAC+TGG + chr1_4:41991708-41991727 Msa0163670:CDS 65.0%
TGGAGGTGGTGCAGCGGAGT+GGG + chr1_4:41991739-41991758 Msa0163670:CDS 65.0%
CGGAGAAACAGCGACGGCGA+CGG + chr1_4:41991794-41991813 Msa0163670:CDS 65.0%
AGAAACAGCGACGGCGACGG+AGG + chr1_4:41991797-41991816 Msa0163670:CDS 65.0%
!! CGTGTCGACTGGCAAGGCGG+TGG + chr1_4:41991719-41991738 Msa0163670:CDS 70.0%
GTCGACTGGCAAGGCGGTGG+AGG + chr1_4:41991722-41991741 Msa0163670:CDS 70.0%
GACTGGCAAGGCGGTGGAGG+TGG + chr1_4:41991725-41991744 Msa0163670:CDS 70.0%
AGGCGGTGGAGGTGGTGCAG+CGG + chr1_4:41991733-41991752 Msa0163670:CDS 70.0%
GTGGAGGTGGTGCAGCGGAG+TGG + chr1_4:41991738-41991757 Msa0163670:CDS 70.0%
GGAGGTGGTGCAGCGGAGTG+GGG + chr1_4:41991740-41991759 Msa0163670:CDS 70.0%
AACAGCGACGGCGACGGAGG+TGG + chr1_4:41991800-41991819 Msa0163670:CDS 70.0%
GGTGGTGCAGCGGAGTGGGG+AGG + chr1_4:41991743-41991762 Msa0163670:CDS 75.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 41991374 41991832 41991374 ID=Msa0163670;Name=Msa0163670
chr1_4 mRNA 41991374 41991832 41991374 ID=Msa0163670-mRNA-1;Parent=Msa0163670;Name=Msa0163670-mRNA-1;_AED=0.18;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|152
chr1_4 exon 41991374 41991832 41991374 ID=Msa0163670-mRNA-1:exon:12175;Parent=Msa0163670-mRNA-1
chr1_4 CDS 41991374 41991832 41991374 ID=Msa0163670-mRNA-1:cds;Parent=Msa0163670-mRNA-1
Gene Sequence

>Msa0163670

ATGATTTGCATTTCCGGCGAATCAGAGGAGGCTGAAACAACCACATTCTCCGCCGTCCACGACGACATAATGCAAACTCACATACTCACACGCCTTGACGGCGCTGCTCTCGCTTCCGCCGCAAGCACCTGCTCCCAACTCAACTCTCTCTGTTCCGACGAACATCTCTGGGCAAACGCATGCTATTCTACGTGGCCTTCCACCAACACGCCACGTGTTCGCCATGTAATCTCAACGTTCTCCGATGGCTCCCGCTCTTTCTTCGCAGACTCTATCTCTTCTCATAGGGGAGGAGTTGAGGCTGAGCTGGATTTTGATCGATCCGGTGAGTCAACGAGCGGTGAACGTGTCGACTGGCAAGGCGGTGGAGGTGGTGCAGCGGAGTGGGGAGGTGAAGTTGCTGTTTGCAACGGTGGTTTACGGAGAAACAGCGACGGCGACGGAGGTGGCGTTGTGTAG

Protein sequence

>Msa0163670

MICISGESEEAETTTFSAVHDDIMQTHILTRLDGAALASAASTCSQLNSLCSDEHLWANACYSTWPSTNTPRVRHVISTFSDGSRSFFADSISSHRGGVEAELDFDRSGESTSGERVDWQGGGGGAAEWGGEVAVCNGGLRRNSDGDGGGVV*