AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1418330


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1418330 MtrunA17_Chr7g0276211 58.678 121 34 4 1 106 1 120 6.30e-30 103
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 37 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCTCCAATTCATCCAATT+TGG 0.162116 tig0024576:-44620 None:intergenic
CGATCATTGGGGTCCCAAAT+TGG 0.282259 tig0024576:+44606 Msa1418330:CDS
TTCTCCAATTCATCCAATTT+GGG 0.339109 tig0024576:-44619 None:intergenic
CACTCTGTATAAACGATCAT+TGG 0.347459 tig0024576:+44593 Msa1418330:CDS
GGGTCCCAAATTGGATGAAT+TGG 0.357124 tig0024576:+44615 Msa1418330:CDS
AGTGGGTATTCAGGCCAATA+TGG 0.382608 tig0024576:+44429 Msa1418330:CDS
GCATCATCGAGTGGGTATTC+AGG 0.385134 tig0024576:+44420 Msa1418330:CDS
AGGAGTCTCTTCCATGCGAC+TGG 0.421499 tig0024576:-44548 None:intergenic
ACTAATCGATGATTCCATAT+TGG 0.431208 tig0024576:-44443 None:intergenic
ACTCTGTATAAACGATCATT+GGG 0.435834 tig0024576:+44594 Msa1418330:CDS
TGTTCCAACGTTGTCATCTC+AGG 0.449613 tig0024576:+44352 Msa1418330:CDS
CTCCTCTGCGCCCAGTCGCA+TGG 0.454617 tig0024576:+44537 Msa1418330:CDS
CCGCCGTGATTGTAGAGAAC+TGG 0.470912 tig0024576:-44395 None:intergenic
CTCAGGAGCACACGTTGTCT+TGG 0.473627 tig0024576:+44369 Msa1418330:CDS
CATGCGACTGGGCGCAGAGG+AGG 0.514470 tig0024576:-44536 None:intergenic
AGGAGCACACGTTGTCTTGG+CGG 0.535212 tig0024576:+44372 Msa1418330:CDS
TAGTGGGTCAGATGTATTGT+GGG 0.536796 tig0024576:+44462 Msa1418330:CDS
ATCAAAGAGTATGTTCTGAA+AGG 0.563818 tig0024576:-44660 None:intergenic
TTCCATGCGACTGGGCGCAG+AGG 0.567243 tig0024576:-44539 None:intergenic
GAGTATGTTCTGAAAGGTGT+AGG 0.567401 tig0024576:-44654 None:intergenic
CGAACAATAACATCGGTATG+AGG 0.567548 tig0024576:-44568 None:intergenic
GTTCTCTACAATCACGGCGG+CGG 0.568781 tig0024576:+44398 Msa1418330:CDS
AATATGGAATCATCGATTAG+TGG 0.570871 tig0024576:+44445 Msa1418330:CDS
GGAGTCTCTTCCATGCGACT+GGG 0.571081 tig0024576:-44547 None:intergenic
ACGGCGGCGGCATCATCGAG+TGG 0.593713 tig0024576:+44411 Msa1418330:CDS
ATATGGAATCATCGATTAGT+GGG 0.595651 tig0024576:+44446 Msa1418330:CDS
CGGCCAGTTCTCTACAATCA+CGG 0.600978 tig0024576:+44392 Msa1418330:CDS
AAAAGGAAAAGATGTGGTGT+TGG 0.611610 tig0024576:+44329 None:intergenic
AATGATCGTTTATACAGAGT+GGG 0.619057 tig0024576:-44591 None:intergenic
CAATGATCGTTTATACAGAG+TGG 0.623120 tig0024576:-44592 None:intergenic
CGGCGGCGGCATCATCGAGT+GGG 0.630862 tig0024576:+44412 Msa1418330:CDS
TTAGTGGGTCAGATGTATTG+TGG 0.640580 tig0024576:+44461 Msa1418330:CDS
TCGCAAGATCAAGATCAAGA+AGG 0.653778 tig0024576:+44507 Msa1418330:CDS
TGCTCCTGAGATGACAACGT+TGG 0.713430 tig0024576:-44356 None:intergenic
CTCTGTATAAACGATCATTG+GGG 0.729290 tig0024576:+44595 Msa1418330:CDS
GAGTGGGCGAACAATAACAT+CGG 0.729674 tig0024576:-44575 None:intergenic
CCAGTTCTCTACAATCACGG+CGG 0.772030 tig0024576:+44395 Msa1418330:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
ACTAATCGATGATTCCATAT+TGG - tig0024576:44446-44465 None:intergenic 30.0%
! AATATGGAATCATCGATTAG+TGG + tig0024576:44445-44464 Msa1418330:CDS 30.0%
! ATATGGAATCATCGATTAGT+GGG + tig0024576:44446-44465 Msa1418330:CDS 30.0%
AATGATCGTTTATACAGAGT+GGG - tig0024576:44594-44613 None:intergenic 30.0%
ACTCTGTATAAACGATCATT+GGG + tig0024576:44594-44613 Msa1418330:CDS 30.0%
TTCTCCAATTCATCCAATTT+GGG - tig0024576:44622-44641 None:intergenic 30.0%
TTTCTCCAATTCATCCAATT+TGG - tig0024576:44623-44642 None:intergenic 30.0%
CAATGATCGTTTATACAGAG+TGG - tig0024576:44595-44614 None:intergenic 35.0%
CACTCTGTATAAACGATCAT+TGG + tig0024576:44593-44612 Msa1418330:CDS 35.0%
CTCTGTATAAACGATCATTG+GGG + tig0024576:44595-44614 Msa1418330:CDS 35.0%
! TTAGTGGGTCAGATGTATTG+TGG + tig0024576:44461-44480 Msa1418330:CDS 40.0%
! TAGTGGGTCAGATGTATTGT+GGG + tig0024576:44462-44481 Msa1418330:CDS 40.0%
TCGCAAGATCAAGATCAAGA+AGG + tig0024576:44507-44526 Msa1418330:CDS 40.0%
CGAACAATAACATCGGTATG+AGG - tig0024576:44571-44590 None:intergenic 40.0%
!! GAGTATGTTCTGAAAGGTGT+AGG - tig0024576:44657-44676 None:intergenic 40.0%
TGTTCCAACGTTGTCATCTC+AGG + tig0024576:44352-44371 Msa1418330:CDS 45.0%
AGTGGGTATTCAGGCCAATA+TGG + tig0024576:44429-44448 Msa1418330:CDS 45.0%
GAGTGGGCGAACAATAACAT+CGG - tig0024576:44578-44597 None:intergenic 45.0%
GGGTCCCAAATTGGATGAAT+TGG + tig0024576:44615-44634 Msa1418330:CDS 45.0%
TGCTCCTGAGATGACAACGT+TGG - tig0024576:44359-44378 None:intergenic 50.0%
CGGCCAGTTCTCTACAATCA+CGG + tig0024576:44392-44411 Msa1418330:CDS 50.0%
CCAGTTCTCTACAATCACGG+CGG + tig0024576:44395-44414 Msa1418330:CDS 50.0%
GCATCATCGAGTGGGTATTC+AGG + tig0024576:44420-44439 Msa1418330:CDS 50.0%
CGATCATTGGGGTCCCAAAT+TGG + tig0024576:44606-44625 Msa1418330:CDS 50.0%
! CTCAGGAGCACACGTTGTCT+TGG + tig0024576:44369-44388 Msa1418330:CDS 55.0%
! AGGAGCACACGTTGTCTTGG+CGG + tig0024576:44372-44391 Msa1418330:CDS 55.0%
CCGCCGTGATTGTAGAGAAC+TGG - tig0024576:44398-44417 None:intergenic 55.0%
GTTCTCTACAATCACGGCGG+CGG + tig0024576:44398-44417 Msa1418330:CDS 55.0%
GGAGTCTCTTCCATGCGACT+GGG - tig0024576:44550-44569 None:intergenic 55.0%
AGGAGTCTCTTCCATGCGAC+TGG - tig0024576:44551-44570 None:intergenic 55.0%
TTCCATGCGACTGGGCGCAG+AGG - tig0024576:44542-44561 None:intergenic 65.0%
ACGGCGGCGGCATCATCGAG+TGG + tig0024576:44411-44430 Msa1418330:CDS 70.0%
CGGCGGCGGCATCATCGAGT+GGG + tig0024576:44412-44431 Msa1418330:CDS 70.0%
CATGCGACTGGGCGCAGAGG+AGG - tig0024576:44539-44558 None:intergenic 70.0%
CTCCTCTGCGCCCAGTCGCA+TGG + tig0024576:44537-44556 Msa1418330:CDS 70.0%
Chromosome Type Strat End Strand Name
tig0024576 gene 44340 44681 44340 ID=Msa1418330;Name=Msa1418330
tig0024576 mRNA 44340 44681 44340 ID=Msa1418330-mRNA-1;Parent=Msa1418330;Name=Msa1418330-mRNA-1;_AED=0.04;_eAED=0.04;_QI=0|-1|0|1|-1|1|1|0|113
tig0024576 exon 44340 44681 44340 ID=Msa1418330-mRNA-1:exon:289;Parent=Msa1418330-mRNA-1
tig0024576 CDS 44340 44681 44340 ID=Msa1418330-mRNA-1:cds;Parent=Msa1418330-mRNA-1
Gene Sequence

>Msa1418330

ATGTGGTGTTGGTGTTCCAACGTTGTCATCTCAGGAGCACACGTTGTCTTGGCGGCCAGTTCTCTACAATCACGGCGGCGGCATCATCGAGTGGGTATTCAGGCCAATATGGAATCATCGATTAGTGGGTCAGATGTATTGTGGGCATTGCAGAAGAGAGCAAGCACTCGCAAGATCAAGATCAAGAAGGAGTCATCCTCCTCTGCGCCCAGTCGCATGGAAGAGACTCCTCATACCGATGTTATTGTTCGCCCACTCTGTATAAACGATCATTGGGGTCCCAAATTGGATGAATTGGAGAAACGTCTTCGTCACCTACACCTTTCAGAACATACTCTTTGA

Protein sequence

>Msa1418330

MWCWCSNVVISGAHVVLAASSLQSRRRHHRVGIQANMESSISGSDVLWALQKRASTRKIKIKKESSSSAPSRMEETPHTDVIVRPLCINDHWGPKLDELEKRLRHLHLSEHTL*