AlfalfaGEDB | Msa0608720

M. sativa cultivar zhongmu-4 / Msa0608720

KEGG: K20069
GO: -
PFAM: PF13649.9

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0608720	AES89766.1	97.838	370	8	0	1	370	1	370	0.0	755

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0608720	G7JCJ7	97.838	370	8	0	1	370	1	370	0.0	755

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000220	Msa0608720	0.821901	3.154730e-53	-8.615850e-47
Msa0000610	Msa0608720	0.812459	4.195951e-51	-8.615850e-47
Msa0000800	Msa0608720	-0.832046	1.182109e-55	-8.615850e-47
Msa0001420	Msa0608720	0.814143	1.789768e-51	-8.615850e-47
Msa0001510	Msa0608720	0.928243	3.804787e-92	-8.615850e-47
Msa0001540	Msa0608720	0.852822	3.587859e-61	-8.615850e-47
Msa0001550	Msa0608720	0.830115	3.522290e-55	-8.615850e-47
Msa0002060	Msa0608720	0.809526	1.811276e-50	-8.615850e-47
Msa0002110	Msa0608720	0.878287	3.171903e-69	-8.615850e-47
Msa0002690	Msa0608720	0.951370	2.354556e-109	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0608720	MtrunA17_Chr4g0041041	97.838	370	8	0	1	370	17	386	0.0	754

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 110 sgRNAs with CRISPR-Local

Find 501 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATGGATACATGCAAATG+GGG	0.624426	4_2:-55933905	Msa0608720:CDS
AACCAGCTTCCTTCAGTGTT+TGG	0.362900	4_2:+55932716	None:intergenic
AAGTAGTACTCGTCAAGAAA+GGG	0.541395	4_2:+55932751	None:intergenic
AATCAATTCTTACCAACCCT+AGG	0.624884	4_2:-55932681	Msa0608720:CDS
AATCAGGGTATACTAAAGAC+TGG	0.564057	4_2:+55940683	None:intergenic
AATCCCAAGATGTTTCATTG+AGG	0.639944	4_2:+55932634	None:intergenic
AATGGTGTATAATCAAGAAT+AGG	0.474995	4_2:-55932500	Msa0608720:three_prime_UTR
ACAAGATCAAATGATTTAGA+AGG	0.410624	4_2:+55933868	None:intergenic
ACATGCAAATGGGGAAGACA+CGG	0.570127	4_2:-55933896	Msa0608720:CDS
ACATTCACTATGGCTCTTGC+AGG	0.387189	4_2:+55933408	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TAGACAAGTTCTTATAAGGT+TGG	-	chr4_2:55932624-55932643	Msa0608720:three_prime_UTR	30.0%
TTGCACAACAAGTTTCAATA+TGG	-	chr4_2:55932681-55932700	Msa0608720:CDS	30.0%
AGAAAAGACCAAAAAGTGAA+AGG	+	chr4_2:55932874-55932893	None:intergenic	30.0%
CATTTCATGCATATGATGAA+GGG	-	chr4_2:55932962-55932981	Msa0608720:intron	30.0%
TGGTAACTCATTATCAAGAA+AGG	+	chr4_2:55933069-55933088	None:intergenic	30.0%
AACATATTCAACTACCAAAG+AGG	+	chr4_2:55933102-55933121	None:intergenic	30.0%
ATTAGCAAAATCACGTGAAA+TGG	-	chr4_2:55933780-55933799	Msa0608720:intron	30.0%
TTATCTTTGGTCTGTACTAT+TGG	+	chr4_2:55934256-55934275	None:intergenic	30.0%
TACAGACCAAAGATAATTTC+TGG	-	chr4_2:55934260-55934279	Msa0608720:CDS	30.0%
TTCCAACTATAGCATAAATC+TGG	-	chr4_2:55934501-55934520	Msa0608720:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_2	gene	55932306	55941232	55932306	ID=Msa0608720;Name=Msa0608720
chr4_2	mRNA	55932306	55941232	55932306	ID=Msa0608720-mRNA-1;Parent=Msa0608720;Name=Msa0608720-mRNA-1;_AED=0.28;_eAED=0.28;_QI=38\|1\|1\|1\|0.87\|0.77\|9\|342\|370
chr4_2	exon	55932306	55932815	55932306	ID=Msa0608720-mRNA-1:exon:14358;Parent=Msa0608720-mRNA-1
chr4_2	exon	55933242	55933262	55933242	ID=Msa0608720-mRNA-1:exon:14357;Parent=Msa0608720-mRNA-1
chr4_2	exon	55933347	55933442	55933347	ID=Msa0608720-mRNA-1:exon:14356;Parent=Msa0608720-mRNA-1
chr4_2	exon	55933852	55934004	55933852	ID=Msa0608720-mRNA-1:exon:14355;Parent=Msa0608720-mRNA-1
chr4_2	exon	55934107	55934340	55934107	ID=Msa0608720-mRNA-1:exon:14354;Parent=Msa0608720-mRNA-1
chr4_2	exon	55940539	55940588	55940539	ID=Msa0608720-mRNA-1:exon:14353;Parent=Msa0608720-mRNA-1
chr4_2	exon	55940683	55940795	55940683	ID=Msa0608720-mRNA-1:exon:14352;Parent=Msa0608720-mRNA-1
chr4_2	exon	55940897	55941187	55940897	ID=Msa0608720-mRNA-1:exon:14351;Parent=Msa0608720-mRNA-1
chr4_2	exon	55941208	55941232	55941208	ID=Msa0608720-mRNA-1:exon:14350;Parent=Msa0608720-mRNA-1
chr4_2	five_prime_UTR	55941208	55941232	55941208	ID=Msa0608720-mRNA-1:five_prime_utr;Parent=Msa0608720-mRNA-1
chr4_2	five_prime_UTR	55941175	55941187	55941175	ID=Msa0608720-mRNA-1:five_prime_utr;Parent=Msa0608720-mRNA-1
chr4_2	CDS	55940897	55941174	55940897	ID=Msa0608720-mRNA-1:cds;Parent=Msa0608720-mRNA-1
chr4_2	CDS	55940683	55940795	55940683	ID=Msa0608720-mRNA-1:cds;Parent=Msa0608720-mRNA-1
chr4_2	CDS	55940539	55940588	55940539	ID=Msa0608720-mRNA-1:cds;Parent=Msa0608720-mRNA-1
chr4_2	CDS	55934107	55934340	55934107	ID=Msa0608720-mRNA-1:cds;Parent=Msa0608720-mRNA-1
chr4_2	CDS	55933852	55934004	55933852	ID=Msa0608720-mRNA-1:cds;Parent=Msa0608720-mRNA-1
chr4_2	CDS	55933347	55933442	55933347	ID=Msa0608720-mRNA-1:cds;Parent=Msa0608720-mRNA-1
chr4_2	CDS	55933242	55933262	55933242	ID=Msa0608720-mRNA-1:cds;Parent=Msa0608720-mRNA-1
chr4_2	CDS	55932648	55932815	55932648	ID=Msa0608720-mRNA-1:cds;Parent=Msa0608720-mRNA-1
chr4_2	three_prime_UTR	55932306	55932647	55932306	ID=Msa0608720-mRNA-1:three_prime_utr;Parent=Msa0608720-mRNA-1

Gene Sequence

>Msa0608720

ATGGCTCTGTCTTCACAACTTCAGAACCTCTCATCATTAATCTCCCCGCATGGCATCACCACCACTACCACAAAGGCACCAACACGGCGTCACGTGGTGGTTCACGCTGCGGCTTCCGACACAACAACCTCCCTTCAAGCTAGTAGCTATGAAGAAGGAAAGCTGGTAAGACCAAAATGGACTGGAGAGACTCCTCTCTCTCGCATGGTTCGAGCTCTCATATCTTTCAAGCCTTTATACTCTATCCTCAAGCTTGGTGCTAGACAAGTTCTTATAAGTACAGCTGAGAAAAACAACATACCTTGGAGGGAAATGACAAAAGAGATTTTGGAGTCAGAAGTTTATAAGGAGTTGGATAGCATTCAAAACCAGTCTTTAGTATACCCTGATTATTATTTGAATCCATTTCATGCATATGATGAAGGGAATCTTACATGGCTGGCAGCAGCAGAAGCCGAGGCTGCTACTAAGTCAATGGCAAGACGAGCGATTCCTACCGCTTCATCAGTAGAAGAAGCGACGCAGATAATGCGTGACAATTGGCTTAATGCAATTGAACAACATCATCTACAGCATTCACAAACATCTATGATTGGTGACATTCTAGACATTGGATGTTCTGTGGGAGTAAGCTCAAGATTTCTTGCGGACAAGTTCCCTACGGCCAATGTCATTGGGCTAGATATGTCACCCTATTTTCTAGCTGTGGCTCAGCATAAGGAAAAGAGCGGAACGCCTAGAAAAAATCCTATAAAATGGATACATGCAAATGGGGAAGACACGGGCTTGCCTTCTAAATCATTTGATCTTGTGTCTATTTCTTATGTGTTTCATGAATGTCCTGCAAGAGCCATAGTGAATGTAGTAAAGGAAGCATTCCGTCTCCTTCGGCCTGGAGGCACGTTTGCTATGACAGATAATTCGCCAAAGTCAAAGGTCCTACAGGAGTTGTCTCCTGTCCTGTTTACATTGATGAAGAGCACAGAACCCTTTCTTGACGAGTACTACTTGACTGATATGGACCAAACACTGAAGGAAGCTGGTTTCGTGAACATAAAATCAATTCTTACCAACCCTAGGCACGTGACTTTAACGGCTACTGTGCCTCAATGA

Protein sequence

>Msa0608720

MALSSQLQNLSSLISPHGITTTTTKAPTRRHVVVHAAASDTTTSLQASSYEEGKLVRPKWTGETPLSRMVRALISFKPLYSILKLGARQVLISTAEKNNIPWREMTKEILESEVYKELDSIQNQSLVYPDYYLNPFHAYDEGNLTWLAAAEAEAATKSMARRAIPTASSVEEATQIMRDNWLNAIEQHHLQHSQTSMIGDILDIGCSVGVSSRFLADKFPTANVIGLDMSPYFLAVAQHKEKSGTPRKNPIKWIHANGEDTGLPSKSFDLVSISYVFHECPARAIVNVVKEAFRLLRPGGTFAMTDNSPKSKVLQELSPVLFTLMKSTEPFLDEYYLTDMDQTLKEAGFVNIKSILTNPRHVTLTATVPQ*