AlfalfaGEDB | Msa0828730

M. sativa cultivar zhongmu-4 / Msa0828730

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0828730	AFK33703.1	79.651	172	9	3	3	150	8	177	3.15e-79	246

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0828730	I3S0B1	79.651	172	9	3	3	150	8	177	1.50e-79	246

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000220	Msa0828730	0.806688	7.284471e-50	-8.615850e-47
Msa0001510	Msa0828730	0.864105	1.545058e-64	-8.615850e-47
Msa0002060	Msa0828730	0.800217	1.598290e-48	-8.615850e-47
Msa0002110	Msa0828730	0.862528	4.754701e-64	-8.615850e-47
Msa0002690	Msa0828730	0.888103	7.944744e-73	-8.615850e-47
Msa0002700	Msa0828730	0.879234	1.471306e-69	-8.615850e-47
Msa0003340	Msa0828730	0.863707	2.053797e-64	-8.615850e-47
Msa0003450	Msa0828730	0.816551	5.218091e-52	-8.615850e-47
Msa0004180	Msa0828730	0.889049	3.430232e-73	-8.615850e-47
Msa0005190	Msa0828730	0.818121	2.311996e-52	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0828730	MtrunA17_Chr3g0119841	79.070	172	10	3	3	150	8	177	1.43e-82	244

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0828730	AT1G64680.2	61.379	145	38	2	23	150	18	161	3.95e-56	175
Msa0828730	AT1G64680.1	61.379	145	38	2	23	150	18	161	9.81e-56	176

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Find 44 sgRNAs with CRISPR-Local

Find 194 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGGAGCTGGTTCACCAGA+TGG	0.553062	5_4:+1658209	None:intergenic
AAATATGCTCCAAGAGCAAA+TGG	0.490621	5_4:-1658266	Msa0828730:CDS
AAATGGGGTGAAGCAAAAGA+GGG	0.627912	5_4:-1655859	Msa0828730:CDS
AACTCTGCAACCCATCTTGT+TGG	0.430396	5_4:+1656749	None:intergenic
AATGGGGTGAAGCAAAAGAG+GGG	0.736860	5_4:-1655858	Msa0828730:CDS
AATTATTTCCACCAACAAGA+TGG	0.491129	5_4:-1656760	Msa0828730:CDS
ACCTGAGCAGGCGCACCTGG+AGG	0.601220	5_4:+1657958	None:intergenic
AGTAACAAAATCACCTCAGA+AGG	0.568318	5_4:+1656683	None:intergenic
ATGATAATATCCACCTGAGC+AGG	0.514693	5_4:+1657946	None:intergenic
ATGGTGCTACTGCTACCATT+CGG	0.571787	5_4:-1658247	Msa0828730:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AGAAGAAGAAAAAAAAGGGT+TGG	-	chr5_4:1655416-1655435	Msa0828730:CDS	30.0%
TAATATAGATACAGGCATGA+GGG	+	chr5_4:1656628-1656647	None:intergenic	30.0%
ACAATTAATCTTTAGACCGA+AGG	-	chr5_4:1656674-1656693	Msa0828730:intron	30.0%
TAACTACCTTATTGCAGTTT+AGG	-	chr5_4:1656711-1656730	Msa0828730:CDS	30.0%
AATTATTTCCACCAACAAGA+TGG	-	chr5_4:1656735-1656754	Msa0828730:CDS	30.0%
ATTATTTCCACCAACAAGAT+GGG	-	chr5_4:1656736-1656755	Msa0828730:CDS	30.0%
TCAATCCATTTATTTGTCAG+TGG	-	chr5_4:1656978-1656997	Msa0828730:intron	30.0%
TCACGTGTGAAAACAAAAAA+TGG	-	chr5_4:1657499-1657518	Msa0828730:intron	30.0%
TATGTACTAGGTTATAGAAG+TGG	-	chr5_4:1657609-1657628	Msa0828730:intron	30.0%
GTTATAGAAGTGGAGATAAA+TGG	-	chr5_4:1657619-1657638	Msa0828730:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_4	gene	1655181	1658336	1655181	ID=Msa0828730;Name=Msa0828730
chr5_4	mRNA	1655181	1658336	1655181	ID=Msa0828730-mRNA-1;Parent=Msa0828730;Name=Msa0828730-mRNA-1;_AED=0.14;_eAED=0.14;_QI=0\|0.4\|0.33\|1\|0.4\|0.5\|6\|232\|161
chr5_4	exon	1655181	1655451	1655181	ID=Msa0828730-mRNA-1:exon:423;Parent=Msa0828730-mRNA-1
chr5_4	exon	1655857	1655898	1655857	ID=Msa0828730-mRNA-1:exon:422;Parent=Msa0828730-mRNA-1
chr5_4	exon	1656697	1656789	1656697	ID=Msa0828730-mRNA-1:exon:421;Parent=Msa0828730-mRNA-1
chr5_4	exon	1657960	1658019	1657960	ID=Msa0828730-mRNA-1:exon:420;Parent=Msa0828730-mRNA-1
chr5_4	exon	1658070	1658315	1658070	ID=Msa0828730-mRNA-1:exon:419;Parent=Msa0828730-mRNA-1
chr5_4	exon	1658331	1658336	1658331	ID=Msa0828730-mRNA-1:exon:418;Parent=Msa0828730-mRNA-1
chr5_4	CDS	1658331	1658336	1658331	ID=Msa0828730-mRNA-1:cds;Parent=Msa0828730-mRNA-1
chr5_4	CDS	1658070	1658315	1658070	ID=Msa0828730-mRNA-1:cds;Parent=Msa0828730-mRNA-1
chr5_4	CDS	1657960	1658019	1657960	ID=Msa0828730-mRNA-1:cds;Parent=Msa0828730-mRNA-1
chr5_4	CDS	1656697	1656789	1656697	ID=Msa0828730-mRNA-1:cds;Parent=Msa0828730-mRNA-1
chr5_4	CDS	1655857	1655898	1655857	ID=Msa0828730-mRNA-1:cds;Parent=Msa0828730-mRNA-1
chr5_4	CDS	1655413	1655451	1655413	ID=Msa0828730-mRNA-1:cds;Parent=Msa0828730-mRNA-1
chr5_4	three_prime_UTR	1655181	1655412	1655181	ID=Msa0828730-mRNA-1:three_prime_utr;Parent=Msa0828730-mRNA-1

Gene Sequence

>Msa0828730

ATGGTGCTTGTTCCTCAGCTAATAACTCCACCAAAATATGCTCCAAGAGCAAATGGTGCTACTGCTACCATTCGGTGTGGTATAGCTGAACCATCTGGTGAACCAGCTCCTTTAGGACAAAAGACACGTTACAATGACAGTATTTTTGAGAAGGTTTTCATGACTTTGTTTGCACGAAAAATGGAACCATTTGCAGAGCCTGTAATAGGGAATGCTAAAAAGAAGAAGAAAAAAAAGGGTTGGTTTATGAGACAACAGCAAGTTGTTCGTGAGGTTCTTCTTTCTATGCTTCCTCCAGGTGCGCCTGCTCAGTTTAGGAAATTATTTCCACCAACAAGATGGGTTGCAGAGTTTAATGCTGCATTGACAGTGCCTTTCTTCCACTGGCTAGTGGGTCCTTCTGAGGTTATAGAAGTGGAGATAAATGGGGTGAAGCAAAAGAGGGGCGTACCTGGAGAACAGTGGATGTGTTGGACAGTGTGTTAA

Protein sequence

>Msa0828730

MVLVPQLITPPKYAPRANGATATIRCGIAEPSGEPAPLGQKTRYNDSIFEKVFMTLFARKMEPFAEPVIGNAKKKKKKKGWFMRQQQVVREVLLSMLPPGAPAQFRKLFPPTRWVAEFNAALTVPFFHWLVGPSEVIEVEINGVKQKRGVPGEQWMCWTVC*