AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1375120


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1375120 MtrunA17_Chr1g0183221 100.000 116 0 0 1 116 67 182 3.43e-84 243
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1375120 AT4G28660.1 79.825 114 23 0 2 115 68 181 2.20e-68 203
Msa1375120 AT4G28660.2 79.825 114 23 0 2 115 83 196 3.65e-68 203

Find 0 sgRNAs with CRISPR-Local

Find 31 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CCTTGAATGAATTGAATTTT+GGG - tig0019645:23-42 None:intergenic 25.0%
!! TCCTTGAATGAATTGAATTT+TGG - tig0019645:24-43 None:intergenic 25.0%
!! TGGTTTTTATATGATTGATG+AGG + tig0019645:169-188 Msa1375120:CDS 25.0%
!! TTTTATATGATTGATGAGGA+AGG + tig0019645:173-192 Msa1375120:CDS 25.0%
! GTAAATGCTAAATTTGTCAA+CGG + tig0019645:215-234 Msa1375120:CDS 25.0%
CCCAAAATTCAATTCATTCA+AGG + tig0019645:20-39 Msa1375120:CDS 30.0%
AAATCAAAGGATGGAACAAA+TGG + tig0019645:80-99 Msa1375120:CDS 30.0%
CATTTACATCAACAGATTGA+AGG - tig0019645:202-221 None:intergenic 30.0%
TTGAAGCCAAGTATATAATG+CGG + tig0019645:252-271 Msa1375120:CDS 30.0%
TGTGAAGCTGACAAAATCAA+AGG + tig0019645:67-86 Msa1375120:CDS 35.0%
AAGCTGACAAAATCAAAGGA+TGG + tig0019645:71-90 Msa1375120:CDS 35.0%
CCAGAAGAATCAAAAACTGA+GGG - tig0019645:131-150 None:intergenic 35.0%
TCCAGAAGAATCAAAAACTG+AGG - tig0019645:132-151 None:intergenic 35.0%
! TTTGATTCTTCTGGAGAAGT+AGG + tig0019645:137-156 Msa1375120:CDS 35.0%
! GATTTTGTCAGCTTCACATC+AGG - tig0019645:65-84 None:intergenic 40.0%
AAAGGATGGAACAAATGGCA+TGG + tig0019645:85-104 Msa1375120:CDS 40.0%
!! CCCTCAGTTTTTGATTCTTC+TGG + tig0019645:128-147 Msa1375120:CDS 40.0%
TACTTGGCTTCAATTCCTGA+GGG - tig0019645:245-264 None:intergenic 40.0%
ATACTTGGCTTCAATTCCTG+AGG - tig0019645:246-265 None:intergenic 40.0%
TGGAGTCCGCATTATATACT+TGG - tig0019645:261-280 None:intergenic 40.0%
AGTATATAATGCGGACTCCA+CGG + tig0019645:261-280 Msa1375120:CDS 40.0%
GGATAGGTTCATGAGATTCA+TGG + tig0019645:289-308 Msa1375120:CDS 40.0%
CAAATGGCATGGCTATCTTC+AGG + tig0019645:96-115 Msa1375120:CDS 45.0%
GTATATAATGCGGACTCCAC+GGG + tig0019645:262-281 Msa1375120:CDS 45.0%
GAGCGATACTCTGATGCTAA+TGG + tig0019645:311-330 Msa1375120:CDS 45.0%
GGAGAAGTAGGCGACATTAC+TGG + tig0019645:149-168 Msa1375120:CDS 50.0%
TTTGTCAACGGAAAGCCCTC+AGG + tig0019645:227-246 Msa1375120:CDS 50.0%
ATGAACCTATCCCAGTCCCG+TGG - tig0019645:281-300 None:intergenic 55.0%
TAATGCGGACTCCACGGGAC+TGG + tig0019645:267-286 Msa1375120:CDS 60.0%
AATGCGGACTCCACGGGACT+GGG + tig0019645:268-287 Msa1375120:CDS 60.0%
GGACTCCACGGGACTGGGAT+AGG + tig0019645:273-292 Msa1375120:CDS 65.0%
Chromosome Type Strat End Strand Name
tig0019645 gene 5 355 5 ID=Msa1375120;Name=Msa1375120
tig0019645 mRNA 5 355 5 ID=Msa1375120-mRNA-1;Parent=Msa1375120;Name=Msa1375120-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|-1|0|1|-1|1|1|0|116
tig0019645 exon 5 355 5 ID=Msa1375120-mRNA-1:exon:4319;Parent=Msa1375120-mRNA-1
tig0019645 CDS 5 355 5 ID=Msa1375120-mRNA-1:cds;Parent=Msa1375120-mRNA-1
Gene Sequence

>Msa1375120

ATGATGGCGGGGAAGCCCAAAATTCAATTCATTCAAGGAACTGATGAACTGACAATACCTGATGTGAAGCTGACAAAATCAAAGGATGGAACAAATGGCATGGCTATCTTCAGGTTTGATCAACCCTCAGTTTTTGATTCTTCTGGAGAAGTAGGCGACATTACTGGTTTTTATATGATTGATGAGGAAGGAGTCCTTCAATCTGTTGATGTAAATGCTAAATTTGTCAACGGAAAGCCCTCAGGAATTGAAGCCAAGTATATAATGCGGACTCCACGGGACTGGGATAGGTTCATGAGATTCATGGAGCGATACTCTGATGCTAATGGTTTGCAATTCATAAAATATTGA

Protein sequence

>Msa1375120

MMAGKPKIQFIQGTDELTIPDVKLTKSKDGTNGMAIFRFDQPSVFDSSGEVGDITGFYMIDEEGVLQSVDVNAKFVNGKPSGIEAKYIMRTPRDWDRFMRFMERYSDANGLQFIKY*