AlfalfaGEDB | Msa0719330

M. sativa cultivar zhongmu-4 / Msa0719330

KEGG: K00855
GO: GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
PFAM: PF00485.21

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0719330	XP_003612664.1	99.270	411	3	0	1	411	1	411	0.0	849

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0719330	sp\|P27774\|KPPR_MESCR	86.911	382	46	2	17	398	7	384	0.0	695

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0719330	G7K882	99.270	411	3	0	1	411	1	411	0.0	849

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000220	Msa0719330	0.851099	1.106201e-60	-8.615850e-47
Msa0000610	Msa0719330	0.842225	2.944793e-58	-8.615850e-47
Msa0000800	Msa0719330	-0.817471	3.241037e-52	-8.615850e-47
Msa0001040	Msa0719330	0.869109	3.961337e-66	-8.615850e-47
Msa0001510	Msa0719330	0.906427	1.501220e-80	-8.615850e-47
Msa0001540	Msa0719330	0.822832	1.917688e-53	-8.615850e-47
Msa0001550	Msa0719330	0.809578	1.765697e-50	-8.615850e-47
Msa0002110	Msa0719330	0.906467	1.438920e-80	-8.615850e-47
Msa0002690	Msa0719330	0.944135	3.398819e-103	-8.615850e-47
Msa0002700	Msa0719330	0.933142	2.943386e-95	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0719330	MtrunA17_Chr5g0409821	99.270	411	3	0	1	411	1	411	0.0	849
Msa0719330	MtrunA17_Chr4g0057631	30.645	124	83	2	137	257	90	213	7.50e-13	70.1
Msa0719330	MtrunA17_Chr7g0225691	28.302	159	111	2	137	292	110	268	9.33e-13	69.7
Msa0719330	MtrunA17_Chr6g0457931	36.667	90	56	1	169	257	5	94	1.84e-12	68.2
Msa0719330	MtrunA17_Chr3g0102981	26.752	157	114	1	137	292	105	261	5.49e-12	67.4
Msa0719330	MtrunA17_Chr5g0447081	26.415	159	114	2	137	292	105	263	3.52e-11	64.7
Msa0719330	MtrunA17_Chr4g0037431	25.907	193	112	6	66	253	90	256	5.24e-11	64.7

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0719330	AT1G32060.1	80.872	413	59	5	1	411	1	395	0.0	679
Msa0719330	AT3G27440.1	24.681	235	154	5	64	293	28	244	1.04e-15	79.3
Msa0719330	AT3G27440.2	24.681	235	154	5	64	293	28	244	1.39e-15	78.6
Msa0719330	AT3G27440.3	25.843	178	129	3	119	293	20	197	1.72e-14	75.1
Msa0719330	AT3G27190.1	33.065	124	80	2	137	257	119	242	2.84e-14	74.7
Msa0719330	AT1G55810.7	26.000	200	124	5	64	257	33	214	8.92e-14	73.2
Msa0719330	AT1G55810.2	26.000	200	124	5	64	257	43	224	9.43e-14	73.2
Msa0719330	AT1G55810.5	30.645	124	83	2	137	257	101	224	9.56e-14	73.2
Msa0719330	AT1G55810.4	30.645	124	83	2	137	257	101	224	9.56e-14	73.2
Msa0719330	AT1G55810.3	26.000	200	124	5	64	257	43	224	1.05e-13	73.2

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Find 99 sgRNAs with CRISPR-Local

Find 232 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACTTACATGCTCTTATCC+TGG	0.529194	5_1:-14406133	Msa0719330:CDS
AACACATAAAATATATGAGG+TGG	0.602665	5_1:+14408202	None:intergenic
AACATACAATACATGAAATG+TGG	0.565555	5_1:+14404631	None:intergenic
AACCTCTCTTCCAAATTCTA+TGG	0.381744	5_1:-14404900	Msa0719330:CDS
AAGAAGAAGTGAGTTTAAGT+TGG	0.366190	5_1:+14408137	None:intergenic
AAGGAAAGATATTAAGAGTG+AGG	0.539424	5_1:-14406243	Msa0719330:CDS
AAGTTGGATAGAGGATTATT+TGG	0.283269	5_1:+14408153	None:intergenic
AATATTTCAGAGAGACATGG+CGG	0.751503	5_1:-14406748	Msa0719330:intron
ACAAATATTTCAGAGAGACA+TGG	0.624789	5_1:-14406751	Msa0719330:intron
ACAAGTTAAAGCTATTAAAG+AGG	0.546142	5_1:-14407621	Msa0719330:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AGTTGGATAGAGGATTATTT+GGG	+	chr5_1:14404620-14404639	None:intergenic	30.0%
AAGTTGGATAGAGGATTATT+TGG	+	chr5_1:14404621-14404640	None:intergenic	30.0%
AAGAAGAAGTGAGTTTAAGT+TGG	+	chr5_1:14404637-14404656	None:intergenic	30.0%
ATTGAGATGTTTGTTCTTAG+AGG	+	chr5_1:14404743-14404762	None:intergenic	30.0%
ACATCATCAAAAACACATGT+TGG	-	chr5_1:14404767-14404786	Msa0719330:CDS	30.0%
TTTGGATGATTACCATTCTT+TGG	-	chr5_1:14405054-14405073	Msa0719330:intron	30.0%
TATTAAACCACCCAAAATCT+TGG	-	chr5_1:14405234-14405253	Msa0719330:intron	30.0%
AATCTTGAGAATCAAGATGT+GGG	-	chr5_1:14405547-14405566	Msa0719330:intron	30.0%
TCAATCTTCCTTAATGTATG+TGG	-	chr5_1:14405666-14405685	Msa0719330:intron	30.0%
CTTATGCAATTCTATAAGGT+TGG	-	chr5_1:14405956-14405975	Msa0719330:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_1	gene	14404449	14408344	14404449	ID=Msa0719330;Name=Msa0719330
chr5_1	mRNA	14404449	14408344	14404449	ID=Msa0719330-mRNA-1;Parent=Msa0719330;Name=Msa0719330-mRNA-1;_AED=0.17;_eAED=0.17;_QI=261\|1\|1\|1\|1\|1\|5\|294\|411
chr5_1	exon	14404449	14404994	14404449	ID=Msa0719330-mRNA-1:exon:5674;Parent=Msa0719330-mRNA-1
chr5_1	exon	14406090	14406334	14406090	ID=Msa0719330-mRNA-1:exon:5673;Parent=Msa0719330-mRNA-1
chr5_1	exon	14406676	14406760	14406676	ID=Msa0719330-mRNA-1:exon:5672;Parent=Msa0719330-mRNA-1
chr5_1	exon	14406872	14406956	14406872	ID=Msa0719330-mRNA-1:exon:5671;Parent=Msa0719330-mRNA-1
chr5_1	exon	14407515	14408344	14407515	ID=Msa0719330-mRNA-1:exon:5670;Parent=Msa0719330-mRNA-1
chr5_1	five_prime_UTR	14408084	14408344	14408084	ID=Msa0719330-mRNA-1:five_prime_utr;Parent=Msa0719330-mRNA-1
chr5_1	CDS	14407515	14408083	14407515	ID=Msa0719330-mRNA-1:cds;Parent=Msa0719330-mRNA-1
chr5_1	CDS	14406872	14406956	14406872	ID=Msa0719330-mRNA-1:cds;Parent=Msa0719330-mRNA-1
chr5_1	CDS	14406676	14406760	14406676	ID=Msa0719330-mRNA-1:cds;Parent=Msa0719330-mRNA-1
chr5_1	CDS	14406090	14406334	14406090	ID=Msa0719330-mRNA-1:cds;Parent=Msa0719330-mRNA-1
chr5_1	CDS	14404743	14404994	14404743	ID=Msa0719330-mRNA-1:cds;Parent=Msa0719330-mRNA-1
chr5_1	three_prime_UTR	14404449	14404742	14404449	ID=Msa0719330-mRNA-1:three_prime_utr;Parent=Msa0719330-mRNA-1

Gene Sequence

>Msa0719330

ATGGCAGCATGTACTGTCTACTCAACACAACCTCTAAGAACAAACATCTCAATTCCAACATCATCAAAAACACATGTTGGTTTGAATCAAAAACAGGTAGTGTTCTACACAACAACCAAAAAGAGCAACAACAGAAGAACTAACAGTAACAACAGAAGATACTTGATAACATGTGGAGCAGGTGACTCACAGACAATTGTGATTGGTCTAGCAGCTGATTCAGGATGTGGAAAAAGTACTTTCATGAGAAGACTCACAAGTGTTTTTGGAGGTGCAGCAGAACCTCCAAAAGGTGGAAACCCTGACTCAAACACACTTATCAGTGACACAACCACTGTGATATGTTTGGATGATTACCATTCTTTGGATAGAACTGGTAGAAAAGAGAAAGGTGTTACTGCACTTGATCCAAGAGCTAATGATTTTGATCTTATGTATGAACAAGTTAAAGCTATTAAAGAGGGTATTTCTGTTCAAAAACCTATTTATAATCATGTTACTGGTCTTTTGGATCCTCCTGAGCTTATTAAACCACCCAAAATCTTGGTCATTGAAGGTCTTCACCCAATGTATGATTCTCGAGTCAGAGATCTTTTGGACTTCAGTATCTACTTAGACATTAGCAATGAGGTGAAATTTGCTTGGAAAATTCAGAGAGACATGGCGGAACGTGGACACAGTCTTGAAAGTATCAAGGCTAGCATTGAAGCAAGGAAGCCTGATTTTGAAGCTTATATTGATCCACAAAAGCAATATGCAGATGCAGTGATAGAAGTGTTGCCAACCCAACTAATTCCAGATGATAATGAAGGAAAGATATTAAGAGTGAGGTTGATCCAGAAAGAGGGTGTTAAATACTTTAGCCCAGTTTACTTGTTTGATGACGGTTCTACAATTTCATGGATACCATGTGGAAGGAAACTTACATGCTCTTATCCTGGCATTAAATTTTCCTATGGACCAGAGACTTACAAAGGAAATGAGGTGTCAGTTGTGGAAATGGATGGACAATTTGACAGACTAGATGAACTAATATATGTTGAGAGCCACTTAAGCAACCTCTCTTCCAAATTCTATGGAGAAGTCACTCAACAAATGTTGAAGCATGCTGATTTCCCTGGTAGCAACAATGGAACAGGTCTCTTCCAAACCATAGTTGGTTTGAAGATAAGAGATTTATTTGAGCAGATAGTAGCAAGCAGGGCTGAGACTCCAGTAGGAGCAGCTAAAGCTTAG

Protein sequence

>Msa0719330

MAACTVYSTQPLRTNISIPTSSKTHVGLNQKQVVFYTTTKKSNNRRTNSNNRRYLITCGAGDSQTIVIGLAADSGCGKSTFMRRLTSVFGGAAEPPKGGNPDSNTLISDTTTVICLDDYHSLDRTGRKEKGVTALDPRANDFDLMYEQVKAIKEGISVQKPIYNHVTGLLDPPELIKPPKILVIEGLHPMYDSRVRDLLDFSIYLDISNEVKFAWKIQRDMAERGHSLESIKASIEARKPDFEAYIDPQKQYADAVIEVLPTQLIPDDNEGKILRVRLIQKEGVKYFSPVYLFDDGSTISWIPCGRKLTCSYPGIKFSYGPETYKGNEVSVVEMDGQFDRLDELIYVESHLSNLSSKFYGEVTQQMLKHADFPGSNNGTGLFQTIVGLKIRDLFEQIVASRAETPVGAAKA*