AlfalfaGEDB | Msa0185540

M. sativa cultivar zhongmu-4 / Msa0185540

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0185540	XP_003604095.2	100.000	170	0	0	1	170	224	393	7.69e-118	348

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0185540	sp\|O65502\|HC244_ARATH	91.765	170	14	0	1	170	226	395	1.71e-112	328

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0185540	G7J9A2	100.000	170	0	0	1	170	224	393	3.67e-118	348

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000220	Msa0185540	0.833023	6.765682e-56	-8.615850e-47
Msa0001420	Msa0185540	0.801735	7.825325e-49	-8.615850e-47
Msa0001510	Msa0185540	0.905454	4.222438e-80	-8.615850e-47
Msa0001540	Msa0185540	0.847945	8.384697e-60	-8.615850e-47
Msa0002060	Msa0185540	0.829414	5.217674e-55	-8.615850e-47
Msa0002110	Msa0185540	0.867757	1.081735e-65	-8.615850e-47
Msa0002690	Msa0185540	0.908725	1.249196e-81	-8.615850e-47
Msa0002700	Msa0185540	0.895027	1.414841e-75	-8.615850e-47
Msa0003340	Msa0185540	0.864586	1.092742e-64	-8.615850e-47
Msa0004180	Msa0185540	0.939563	1.030058e-99	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0185540	MtrunA17_Chr3g0146001	100.000	170	0	0	1	170	224	393	7.07e-122	348

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0185540	AT4G35250.1	91.765	170	14	0	1	170	226	395	1.74e-113	328

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Find 40 sgRNAs with CRISPR-Local

Find 194 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATATCTGATTGTTTCGAC+TGG	0.443973	1_4:-75861750	None:intergenic
AAATGATGTTGCTGACAGAT+TGG	0.529217	1_4:+75861080	Msa0185540:CDS
AATATCTGATTGTTTCGACT+GGG	0.571429	1_4:-75861749	None:intergenic
AATCGCTTACATGGATACTC+AGG	0.576431	1_4:+75860761	Msa0185540:CDS
ACACAATGTAATCACCTCTT+GGG	0.536741	1_4:-75860965	None:intergenic
ACATTGTGTGAGAGGCTTGC+GGG	0.395591	1_4:+75860979	Msa0185540:CDS
AGGTGATTACATTGTGTGAG+AGG	0.579608	1_4:+75860971	Msa0185540:CDS
AGGTTGTATGTCTCCGCCAT+TGG	0.538477	1_4:-75861634	None:intergenic
AGTATCCATGTAAGCGATTC+TGG	0.290196	1_4:-75860757	None:intergenic
ATCCATGTAAGCGATTCTGG+TGG	0.627734	1_4:-75860754	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTCTCGAAGAAAAATCTGTT+TGG	+	chr1_4:75860718-75860737	Msa0185540:CDS	30.0%
TCTCGAAGAAAAATCTGTTT+GGG	+	chr1_4:75860719-75860738	Msa0185540:CDS	30.0%
TGCATTACTACATTTCCATT+GGG	+	chr1_4:75861316-75861335	Msa0185540:intron	30.0%
GGAATAAACCTGTAAGAATA+GGG	-	chr1_4:75861428-75861447	None:intergenic	30.0%
TGGAATAAACCTGTAAGAAT+AGG	-	chr1_4:75861429-75861448	None:intergenic	30.0%
ACAGGTTTATTCCATTCTAT+TGG	+	chr1_4:75861435-75861454	Msa0185540:intron	30.0%
CATGCCTATAGTTGATTATA+CGG	+	chr1_4:75861568-75861587	Msa0185540:intron	30.0%
ATGCCTATAGTTGATTATAC+GGG	+	chr1_4:75861569-75861588	Msa0185540:intron	30.0%
TTTCCCGTATAATCAACTAT+AGG	-	chr1_4:75861575-75861594	None:intergenic	30.0%
ATTATACGGGAAATATTTGC+AGG	+	chr1_4:75861582-75861601	Msa0185540:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_4	gene	75860684	75862215	75860684	ID=Msa0185540;Name=Msa0185540
chr1_4	mRNA	75860684	75862215	75860684	ID=Msa0185540-mRNA-1;Parent=Msa0185540;Name=Msa0185540-mRNA-1;_AED=0.06;_eAED=0.06;_QI=0\|1\|0.33\|1\|1\|1\|3\|435\|170
chr1_4	exon	75860684	75860782	75860684	ID=Msa0185540-mRNA-1:exon:25834;Parent=Msa0185540-mRNA-1
chr1_4	exon	75860877	75861113	75860877	ID=Msa0185540-mRNA-1:exon:25835;Parent=Msa0185540-mRNA-1
chr1_4	exon	75861604	75862215	75861604	ID=Msa0185540-mRNA-1:exon:25836;Parent=Msa0185540-mRNA-1
chr1_4	CDS	75860684	75860782	75860684	ID=Msa0185540-mRNA-1:cds;Parent=Msa0185540-mRNA-1
chr1_4	CDS	75860877	75861113	75860877	ID=Msa0185540-mRNA-1:cds;Parent=Msa0185540-mRNA-1
chr1_4	CDS	75861604	75861780	75861604	ID=Msa0185540-mRNA-1:cds;Parent=Msa0185540-mRNA-1
chr1_4	three_prime_UTR	75861781	75862215	75861781	ID=Msa0185540-mRNA-1:three_prime_utr;Parent=Msa0185540-mRNA-1

Gene Sequence

>Msa0185540

ATGCAGGGTCTTATTGGCCAGTATGCTGTTCCTATTCTCGAAGAAAAATCTGTTTGGGGAACTGACGCCCCCACCAGAATCGCTTACATGGATACTCAGGATATAGCTCGCTTGACTTTTATAGCCTTACGAAATGAAAAAATCAATGGTAAACTTCTTACATTTGCTGGTCCTCGTGCGTGGACTACCCAAGAGGTGATTACATTGTGTGAGAGGCTTGCGGGCCAAGATGCTAATGTAACTACAGTTCCTGTCTCCGTTTTAAGACTCACTCGTCAGTTAACCCGCTTTTTTGAGTGGACAAATGATGTTGCTGACAGATTGGCATTTTCAGAGGTGCTTACAAGTGACACAGTTTTCTCTGTTCCAATGGCGGAGACATACAACCTACTTGGTGTCGATACCAAAGATATAATTACATTAGAGAAGTATTTGCAGGACTACTTCACGAACATATTGAAGAAGTTGAAGGATCTCAAAGCCCAGTCGAAACAATCAGATATTTTCTTCTGA

Protein sequence

>Msa0185540

MQGLIGQYAVPILEEKSVWGTDAPTRIAYMDTQDIARLTFIALRNEKINGKLLTFAGPRAWTTQEVITLCERLAGQDANVTTVPVSVLRLTRQLTRFFEWTNDVADRLAFSEVLTSDTVFSVPMAETYNLLGVDTKDIITLEKYLQDYFTNILKKLKDLKAQSKQSDIFF*