AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280006764.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280006764.01.T01 MTR_2g014430 95.270 148 7 0 3 150 1 148 1.29e-84 244
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280006764.01.T01 AT2G33855 55.944 143 61 1 3 145 1 141 8.13e-48 151

Find 65 sgRNAs with CRISPR-Local

Find 73 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGAGGGTTGGTGTGGTTGT+GGG 0.269536 2:+5957696 None:intergenic
GGTTTGTAAGGATGTGTGTT+AGG 0.271091 2:+5957718 None:intergenic
GTGGAGGGTTGGTGTGGTTG+TGG 0.275633 2:+5957695 None:intergenic
GTGTGGTTGTGGGGTTTGTA+AGG 0.288036 2:+5957706 None:intergenic
TATCAATCAAGATAACTAAA+TGG 0.290563 2:+5957327 None:intergenic
TGGGTATTCCTAAAACTTCT+TGG 0.291474 2:-5957545 MsG0280006764.01.T01:CDS
GGTAATAAAATAGGTATAAG+AGG 0.312742 2:+5957384 None:intergenic
AGTGACAACAACAAAATATT+AGG 0.321966 2:+5957363 None:intergenic
TGTTGTTGTCACTTATGATA+TGG 0.343100 2:-5957353 MsG0280006764.01.T01:CDS
AAAATATTAGGTAATAAAAT+AGG 0.344419 2:+5957375 None:intergenic
AATTATCATTCTTGCTCAAA+AGG 0.344967 2:-5957578 MsG0280006764.01.T01:CDS
AAATCCCCTGCTACCTCAAA+TGG 0.355642 2:-5957650 MsG0280006764.01.T01:CDS
GAATCAATGGTGGAGGTTGC+TGG 0.357663 2:+5957505 None:intergenic
GAGTTTATGAAATTAGAAAT+TGG 0.363694 2:+5957902 None:intergenic
TAAGGATGTGTGTTAGGGCT+TGG 0.394935 2:+5957724 None:intergenic
GTTTGTAAGGATGTGTGTTA+GGG 0.413826 2:+5957719 None:intergenic
AACAAAGGAAGAGTTATGTT+AGG 0.432276 2:-5957446 MsG0280006764.01.T01:CDS
GGAAGAGTTATGTTAGGAAA+AGG 0.438245 2:-5957440 MsG0280006764.01.T01:CDS
AATCAATCTTATACAGGAAG+TGG 0.442092 2:+5957275 None:intergenic
GATGGATTGGAGCAGAGGAT+TGG 0.459996 2:+5957618 None:intergenic
AACTCCTTGTGAACAAGAAA+AGG 0.467568 2:-5957884 MsG0280006764.01.T01:exon
CTCAAAAGGGTCTTCACAAT+GGG 0.476531 2:-5957564 MsG0280006764.01.T01:CDS
GAAGAGATGGATTGGAGCAG+AGG 0.480756 2:+5957613 None:intergenic
GGAGGGTTGGTGTGGTTGTG+GGG 0.493337 2:+5957697 None:intergenic
AGGGTTAACATTTGTGCCAA+GGG 0.493789 2:+5957404 None:intergenic
GGATTGGAGCAGAGGATTGG+TGG 0.495688 2:+5957621 None:intergenic
ATTATCATTCTTGCTCAAAA+GGG 0.496190 2:-5957577 MsG0280006764.01.T01:CDS
GTAGTCCCATTTGAGGTAGC+AGG 0.497802 2:+5957644 None:intergenic
ACTCCTTCAGCAAGAATCAA+TGG 0.503351 2:+5957492 None:intergenic
AGGAGTGGAGGAAGCACGGT+GGG 0.505781 2:-5957475 MsG0280006764.01.T01:CDS
AATGGTGGAGGTTGCTGGAA+TGG 0.508912 2:+5957510 None:intergenic
AATCCCCTGCTACCTCAAAT+GGG 0.518789 2:-5957649 MsG0280006764.01.T01:CDS
ATGTTAGGAAAAGGCTTGCA+AGG 0.547320 2:-5957431 MsG0280006764.01.T01:CDS
GTATTCCTAAAACTTCTTGG+AGG 0.547416 2:-5957542 MsG0280006764.01.T01:CDS
GAGGGTTAACATTTGTGCCA+AGG 0.547578 2:+5957403 None:intergenic
GATTGGAGCAGAGGATTGGT+GGG 0.557001 2:+5957622 None:intergenic
GTAATAAAATAGGTATAAGA+GGG 0.558203 2:+5957385 None:intergenic
GCTCAAAAGGGTCTTCACAA+TGG 0.561078 2:-5957565 MsG0280006764.01.T01:CDS
TAGTCCCATTTGAGGTAGCA+GGG 0.566660 2:+5957645 None:intergenic
ATCAATCTTATACAGGAAGT+GGG 0.572022 2:+5957276 None:intergenic
CCACCATTGATTCTTGCTGA+AGG 0.574245 2:-5957495 MsG0280006764.01.T01:CDS
ATTGATTCTTGCTGAAGGAG+TGG 0.575091 2:-5957490 MsG0280006764.01.T01:CDS
TTTATTCATATATATGACAA+AGG 0.585315 2:-5957230 MsG0280006764.01.T01:exon
CTGAAGGAGTGGAGGAAGCA+CGG 0.592911 2:-5957479 MsG0280006764.01.T01:CDS
TTGAGAGTGGAGGGTTGGTG+TGG 0.593756 2:+5957689 None:intergenic
AAGAATTGAGAGTGGAGGGT+TGG 0.597424 2:+5957684 None:intergenic
AGACAAAAGCTAGAGAAATG+AGG 0.598819 2:+5957783 None:intergenic
TCAGCAAGAATCAATGGTGG+AGG 0.602784 2:+5957498 None:intergenic
TTGGTGGGTAGTCCCATTTG+AGG 0.608890 2:+5957637 None:intergenic
GAGGTAGCAGGGGATTTGTG+TGG 0.609669 2:+5957656 None:intergenic
AGGCTTGCAAGGAAACCCCT+TGG 0.615306 2:-5957420 MsG0280006764.01.T01:CDS
GATTCTTGCTGAAGGAGTGG+AGG 0.617592 2:-5957487 MsG0280006764.01.T01:CDS
AGGTTGCTGGAATGGACACT+TGG 0.621740 2:+5957518 None:intergenic
CTATGAAGAATTGAGAGTGG+AGG 0.622479 2:+5957679 None:intergenic
AGTCCCATTTGAGGTAGCAG+GGG 0.633905 2:+5957646 None:intergenic
GGAGTGGAGGAAGCACGGTG+GGG 0.635320 2:-5957474 MsG0280006764.01.T01:CDS
TGGCTATGAAGAATTGAGAG+TGG 0.639401 2:+5957676 None:intergenic
TATGAAGAATTGAGAGTGGA+GGG 0.652100 2:+5957680 None:intergenic
AAGGAGTGGAGGAAGCACGG+TGG 0.655059 2:-5957476 MsG0280006764.01.T01:CDS
CACGGTGGGGAGTTGAACAA+AGG 0.655259 2:-5957461 MsG0280006764.01.T01:CDS
GGGTTAACATTTGTGCCAAG+GGG 0.655878 2:+5957405 None:intergenic
TGAGCATGGAAGATACAACA+TGG 0.667356 2:-5957758 MsG0280006764.01.T01:CDS
CCTTCAGCAAGAATCAATGG+TGG 0.683493 2:+5957495 None:intergenic
GAGCATGGAAGATACAACAT+GGG 0.707943 2:-5957757 MsG0280006764.01.T01:CDS
TTCGCATAATAGATAAAACG+AGG 0.722986 2:+5957812 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATATTAGGTAATAAAAT+AGG + Chr2:5957722-5957741 None:intergenic 10.0%
!!! TATAAGATTGATTTTTATTT+TGG - Chr2:5957829-5957848 MsG0280006764.01.T01:exon 10.0%
!! AATAAAAATCAATCTTATAC+AGG + Chr2:5957828-5957847 None:intergenic 15.0%
!! TTTATTCATATATATGACAA+AGG - Chr2:5957864-5957883 MsG0280006764.01.T01:exon 15.0%
!! GTAATAAAATAGGTATAAGA+GGG + Chr2:5957712-5957731 None:intergenic 20.0%
!! TATCAATCAAGATAACTAAA+TGG + Chr2:5957770-5957789 None:intergenic 20.0%
! AATTATCATTCTTGCTCAAA+AGG - Chr2:5957516-5957535 MsG0280006764.01.T01:CDS 25.0%
! AGTGACAACAACAAAATATT+AGG + Chr2:5957734-5957753 None:intergenic 25.0%
! ATTATCATTCTTGCTCAAAA+GGG - Chr2:5957517-5957536 MsG0280006764.01.T01:CDS 25.0%
! GGTAATAAAATAGGTATAAG+AGG + Chr2:5957713-5957732 None:intergenic 25.0%
AACAAAGGAAGAGTTATGTT+AGG - Chr2:5957648-5957667 MsG0280006764.01.T01:CDS 30.0%
AATCAATCTTATACAGGAAG+TGG + Chr2:5957822-5957841 None:intergenic 30.0%
ATCAATCTTATACAGGAAGT+GGG + Chr2:5957821-5957840 None:intergenic 30.0%
ATCCATCTCTTCTCAAAAAA+TGG - Chr2:5957492-5957511 MsG0280006764.01.T01:CDS 30.0%
TCCATCTCTTCTCAAAAAAT+GGG - Chr2:5957493-5957512 MsG0280006764.01.T01:CDS 30.0%
TTCGCATAATAGATAAAACG+AGG + Chr2:5957285-5957304 None:intergenic 30.0%
! TGTTGTTGTCACTTATGATA+TGG - Chr2:5957741-5957760 MsG0280006764.01.T01:CDS 30.0%
!!! TTTTTTGAGAAGAGATGGAT+TGG + Chr2:5957492-5957511 None:intergenic 30.0%
AACTCCTTGTGAACAAGAAA+AGG - Chr2:5957210-5957229 MsG0280006764.01.T01:exon 35.0%
AGACAAAAGCTAGAGAAATG+AGG + Chr2:5957314-5957333 None:intergenic 35.0%
GGAAGAGTTATGTTAGGAAA+AGG - Chr2:5957654-5957673 MsG0280006764.01.T01:CDS 35.0%
GTATTCCTAAAACTTCTTGG+AGG - Chr2:5957552-5957571 MsG0280006764.01.T01:CDS 35.0%
GTTTGTAAGGATGTGTGTTA+GGG + Chr2:5957378-5957397 None:intergenic 35.0%
TATGAAGAATTGAGAGTGGA+GGG + Chr2:5957417-5957436 None:intergenic 35.0%
TGGGTATTCCTAAAACTTCT+TGG - Chr2:5957549-5957568 MsG0280006764.01.T01:CDS 35.0%
! ATGACCTTTTCTTGTTCACA+AGG + Chr2:5957217-5957236 None:intergenic 35.0%
!!! TCCCATTTTTTGAGAAGAGA+TGG + Chr2:5957497-5957516 None:intergenic 35.0%
ACTCCTTCAGCAAGAATCAA+TGG + Chr2:5957605-5957624 None:intergenic 40.0%
AGGGTTAACATTTGTGCCAA+GGG + Chr2:5957693-5957712 None:intergenic 40.0%
ATGTTAGGAAAAGGCTTGCA+AGG - Chr2:5957663-5957682 MsG0280006764.01.T01:CDS 40.0%
CTATGAAGAATTGAGAGTGG+AGG + Chr2:5957418-5957437 None:intergenic 40.0%
CTCAAAAGGGTCTTCACAAT+GGG - Chr2:5957530-5957549 MsG0280006764.01.T01:CDS 40.0%
GAGCATGGAAGATACAACAT+GGG - Chr2:5957337-5957356 MsG0280006764.01.T01:CDS 40.0%
GGTTTGTAAGGATGTGTGTT+AGG + Chr2:5957379-5957398 None:intergenic 40.0%
TGAGCATGGAAGATACAACA+TGG - Chr2:5957336-5957355 MsG0280006764.01.T01:CDS 40.0%
TGGCTATGAAGAATTGAGAG+TGG + Chr2:5957421-5957440 None:intergenic 40.0%
! ATTGATTCTTGCTGAAGGAG+TGG - Chr2:5957604-5957623 MsG0280006764.01.T01:CDS 40.0%
! TAGCTTTTGTCTGATGAGCA+TGG - Chr2:5957322-5957341 MsG0280006764.01.T01:exon 40.0%
! TTGGACCTCCAAGAAGTTTT+AGG + Chr2:5957560-5957579 None:intergenic 40.0%
AAATCCCCTGCTACCTCAAA+TGG - Chr2:5957444-5957463 MsG0280006764.01.T01:CDS 45.0%
AAGAATTGAGAGTGGAGGGT+TGG + Chr2:5957413-5957432 None:intergenic 45.0%
AATCCCCTGCTACCTCAAAT+GGG - Chr2:5957445-5957464 MsG0280006764.01.T01:CDS 45.0%
CCTTCAGCAAGAATCAATGG+TGG + Chr2:5957602-5957621 None:intergenic 45.0%
GAGGGTTAACATTTGTGCCA+AGG + Chr2:5957694-5957713 None:intergenic 45.0%
GCTCAAAAGGGTCTTCACAA+TGG - Chr2:5957529-5957548 MsG0280006764.01.T01:CDS 45.0%
GGGTTAACATTTGTGCCAAG+GGG + Chr2:5957692-5957711 None:intergenic 45.0%
TAAGGATGTGTGTTAGGGCT+TGG + Chr2:5957373-5957392 None:intergenic 45.0%
TCAGCAAGAATCAATGGTGG+AGG + Chr2:5957599-5957618 None:intergenic 45.0%
! TAGTCCCATTTGAGGTAGCA+GGG + Chr2:5957452-5957471 None:intergenic 45.0%
!! CCACCATTGATTCTTGCTGA+AGG - Chr2:5957599-5957618 MsG0280006764.01.T01:CDS 45.0%
AGGTTGCTGGAATGGACACT+TGG + Chr2:5957579-5957598 None:intergenic 50.0%
GATTCTTGCTGAAGGAGTGG+AGG - Chr2:5957607-5957626 MsG0280006764.01.T01:CDS 50.0%
GTGTGGTTGTGGGGTTTGTA+AGG + Chr2:5957391-5957410 None:intergenic 50.0%
! AATGGTGGAGGTTGCTGGAA+TGG + Chr2:5957587-5957606 None:intergenic 50.0%
! AGTCCCATTTGAGGTAGCAG+GGG + Chr2:5957451-5957470 None:intergenic 50.0%
! GAATCAATGGTGGAGGTTGC+TGG + Chr2:5957592-5957611 None:intergenic 50.0%
! GATTGGAGCAGAGGATTGGT+GGG + Chr2:5957475-5957494 None:intergenic 50.0%
! GTAGTCCCATTTGAGGTAGC+AGG + Chr2:5957453-5957472 None:intergenic 50.0%
! TTGGTGGGTAGTCCCATTTG+AGG + Chr2:5957460-5957479 None:intergenic 50.0%
!! GAAGAGATGGATTGGAGCAG+AGG + Chr2:5957484-5957503 None:intergenic 50.0%
!! GATGGATTGGAGCAGAGGAT+TGG + Chr2:5957479-5957498 None:intergenic 50.0%
AGGCTTGCAAGGAAACCCCT+TGG - Chr2:5957674-5957693 MsG0280006764.01.T01:CDS 55.0%
CACGGTGGGGAGTTGAACAA+AGG - Chr2:5957633-5957652 MsG0280006764.01.T01:CDS 55.0%
CTGAAGGAGTGGAGGAAGCA+CGG - Chr2:5957615-5957634 MsG0280006764.01.T01:CDS 55.0%
GAGGTAGCAGGGGATTTGTG+TGG + Chr2:5957441-5957460 None:intergenic 55.0%
GGATTGGAGCAGAGGATTGG+TGG + Chr2:5957476-5957495 None:intergenic 55.0%
!! TGGAGGGTTGGTGTGGTTGT+GGG + Chr2:5957401-5957420 None:intergenic 55.0%
!! TTGAGAGTGGAGGGTTGGTG+TGG + Chr2:5957408-5957427 None:intergenic 55.0%
! AAGGAGTGGAGGAAGCACGG+TGG - Chr2:5957618-5957637 MsG0280006764.01.T01:CDS 60.0%
! AGGAGTGGAGGAAGCACGGT+GGG - Chr2:5957619-5957638 MsG0280006764.01.T01:CDS 60.0%
!! GGAGGGTTGGTGTGGTTGTG+GGG + Chr2:5957400-5957419 None:intergenic 60.0%
!! GTGGAGGGTTGGTGTGGTTG+TGG + Chr2:5957402-5957421 None:intergenic 60.0%
! GGAGTGGAGGAAGCACGGTG+GGG - Chr2:5957620-5957639 MsG0280006764.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr2 gene 5957195 5957921 5957195 ID=MsG0280006764.01;
Chr2 mRNA 5957195 5957921 5957195 ID=MsG0280006764.01.T01;Parent=MsG0280006764.01
Chr2 exon 5957195 5957921 5957195 ID=MsG0280006764.01.T01.exon;Parent=MsG0280006764.01.T01
Chr2 CDS 5957329 5957781 5957329 ID=MsG0280006764.01.T01.cds;Parent=MsG0280006764.01.T01
Gene Sequence

>MsG0280006764.01.T01

ATGAGCATGGAAGATACAACATGGGAGCAGAAACTCCAAGCCCTAACACACATCCTTACAAACCCCACAACCACACCAACCCTCCACTCTCAATTCTTCATAGCCACACAAATCCCCTGCTACCTCAAATGGGACTACCCACCAATCCTCTGCTCCAATCCATCTCTTCTCAAAAAATGGGAATTATCATTCTTGCTCAAAAGGGTCTTCACAATGGGTATTCCTAAAACTTCTTGGAGGTCCAAGTGTCCATTCCAGCAACCTCCACCATTGATTCTTGCTGAAGGAGTGGAGGAAGCACGGTGGGGAGTTGAACAAAGGAAGAGTTATGTTAGGAAAAGGCTTGCAAGGAAACCCCTTGGCACAAATGTTAACCCTCTTATACCTATTTTATTACCTAATATTTTGTTGTTGTCACTTATGATATGGAATCCATTTAGTTATCTTGATTGA

Protein sequence

>MsG0280006764.01.T01

MSMEDTTWEQKLQALTHILTNPTTTPTLHSQFFIATQIPCYLKWDYPPILCSNPSLLKKWELSFLLKRVFTMGIPKTSWRSKCPFQQPPPLILAEGVEEARWGVEQRKSYVRKRLARKPLGTNVNPLIPILLPNILLLSLMIWNPFSYLD*