AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1243740


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 41 sgRNAs with CRISPR-Local

Find 57 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGCTGCATGTGTTTGTTTA+AGG 0.185584 8_2:-30205038 None:intergenic
GGGTATGGGTTCGGTATTTC+GGG 0.201322 8_2:-30204975 None:intergenic
GGTTCGGTATTTCGGGTTTC+GGG 0.237009 8_2:-30204968 None:intergenic
TGGGTATGGGTTCGGTATTT+CGG 0.277325 8_2:-30204976 None:intergenic
GGGTTCGGTATTTCGGGTTT+CGG 0.298928 8_2:-30204969 None:intergenic
TTCGTTGATGCATGAGAATT+TGG 0.338359 8_2:-30205142 None:intergenic
ACGGGTGAAAGGGGAGGATC+GGG 0.341829 8_2:-30204769 None:intergenic
TATTGTTTAGAATGTTGTTC+TGG 0.349496 8_2:-30204707 None:intergenic
AGTCATCATACTAGGATGTT+TGG 0.350832 8_2:-30205084 None:intergenic
CTGGGAGATTGCTCATGTTA+CGG 0.406540 8_2:-30205283 None:intergenic
GTTTAAGGTGGTTGGTCGGT+CGG 0.432366 8_2:-30205023 None:intergenic
ACGCCGGCGAGAGAGAATAA+TGG 0.446201 8_2:-30204798 None:intergenic
TCGCCGGCGTTCTTCAACTC+AGG 0.455017 8_2:+30204811 Msa1243740:CDS
AACGGGTGAAAGGGGAGGAT+CGG 0.467460 8_2:-30204770 None:intergenic
ATGGAGACGAACGGGTGAAA+GGG 0.471383 8_2:-30204779 None:intergenic
TTCGGTTTAGTCATCATACT+AGG 0.480056 8_2:-30205092 None:intergenic
AGAGAATAATGGAGACGAAC+GGG 0.487256 8_2:-30204787 None:intergenic
GAAGAGATGATGAACGATGA+AGG 0.500398 8_2:-30205256 None:intergenic
ATGTGTTTGTTTAAGGTGGT+TGG 0.513154 8_2:-30205031 None:intergenic
CTGCATGTGTTTGTTTAAGG+TGG 0.521593 8_2:-30205035 None:intergenic
GATCTTAGAATTGCCATGGT+TGG 0.529897 8_2:-30205212 None:intergenic
AGGATGTTTGGATTTGTACA+AGG 0.531120 8_2:-30205072 None:intergenic
AAGGTGGTTGGTCGGTCGGT+CGG 0.531598 8_2:-30205019 None:intergenic
AATGGAGACGAACGGGTGAA+AGG 0.546663 8_2:-30204780 None:intergenic
TGGAGACGAACGGGTGAAAG+GGG 0.549042 8_2:-30204778 None:intergenic
AAGAGATGATGAACGATGAA+GGG 0.552036 8_2:-30205255 None:intergenic
CGGGTGAAAGGGGAGGATCG+GGG 0.563715 8_2:-30204768 None:intergenic
TATTGATCTTAGAATTGCCA+TGG 0.568232 8_2:-30205216 None:intergenic
GGGATCAATTCTATGATACA+AGG 0.586596 8_2:-30204748 None:intergenic
TGTTTAGAATGTTGTTCTGG+TGG 0.590272 8_2:-30204704 None:intergenic
AGACGAACGGGTGAAAGGGG+AGG 0.599156 8_2:-30204775 None:intergenic
GAGAGAATAATGGAGACGAA+CGG 0.610866 8_2:-30204788 None:intergenic
TCTCTCTCTCAAACCAACCA+TGG 0.623502 8_2:+30205199 Msa1243740:three_prime_UTR
TCTCCATTATTCTCTCTCGC+CGG 0.624876 8_2:+30204795 Msa1243740:CDS
GGTCGGTCTTACATTACACA+AGG 0.630545 8_2:-30205002 None:intergenic
GTTTGTTTAAGGTGGTTGGT+CGG 0.635447 8_2:-30205027 None:intergenic
TCGGTCTTACATTACACAAG+GGG 0.650244 8_2:-30205000 None:intergenic
CCAGAAAACACAGTGTACAC+AGG 0.665717 8_2:+30205302 Msa1243740:three_prime_UTR
GTCGGTCTTACATTACACAA+GGG 0.665900 8_2:-30205001 None:intergenic
ATACCTGAGTTGAAGAACGC+CGG 0.670797 8_2:-30204814 None:intergenic
CGGTCTTACATTACACAAGG+GGG 0.759626 8_2:-30204999 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATTCATTATTATTAAAAG+AGG + chr8_2:30204678-30204697 Msa1243740:intron 10.0%
!!! TAAATCTAATTTATTCTTTT+TGG + chr8_2:30204934-30204953 Msa1243740:intron 10.0%
!!! CCTTAAAATTTTGTTTGTTT+CGG - chr8_2:30205113-30205132 None:intergenic 20.0%
! ATGTTACTAGATTCAATGTT+TGG - chr8_2:30204660-30204679 None:intergenic 25.0%
!! TATTGTTTAGAATGTTGTTC+TGG - chr8_2:30204710-30204729 None:intergenic 25.0%
!! TCTGGTAATTTTCTCATTTT+TGG + chr8_2:30204843-30204862 Msa1243740:intron 25.0%
!!! ATTTTCTCATTTTTGGTGAT+TGG + chr8_2:30204850-30204869 Msa1243740:intron 25.0%
! GAGTCTAATTCTAATTAAAC+TGG - chr8_2:30204894-30204913 None:intergenic 25.0%
!! CCGAAACAAACAAAATTTTA+AGG + chr8_2:30205110-30205129 Msa1243740:three_prime_UTR 25.0%
TATTGATCTTAGAATTGCCA+TGG - chr8_2:30205219-30205238 None:intergenic 30.0%
! TGTTTAGAATGTTGTTCTGG+TGG - chr8_2:30204707-30204726 None:intergenic 35.0%
GGGATCAATTCTATGATACA+AGG - chr8_2:30204751-30204770 None:intergenic 35.0%
CAACTCAGGTATATTCAATC+TGG + chr8_2:30204825-30204844 Msa1243740:intron 35.0%
! ATGTGTTTGTTTAAGGTGGT+TGG - chr8_2:30205034-30205053 None:intergenic 35.0%
ATGCTGCATGTGTTTGTTTA+AGG - chr8_2:30205041-30205060 None:intergenic 35.0%
AGGATGTTTGGATTTGTACA+AGG - chr8_2:30205075-30205094 None:intergenic 35.0%
AGTCATCATACTAGGATGTT+TGG - chr8_2:30205087-30205106 None:intergenic 35.0%
TTCGGTTTAGTCATCATACT+AGG - chr8_2:30205095-30205114 None:intergenic 35.0%
TTCGTTGATGCATGAGAATT+TGG - chr8_2:30205145-30205164 None:intergenic 35.0%
AAGAGATGATGAACGATGAA+GGG - chr8_2:30205258-30205277 None:intergenic 35.0%
! AGAGAATAATGGAGACGAAC+GGG - chr8_2:30204790-30204809 None:intergenic 40.0%
! GAGAGAATAATGGAGACGAA+CGG - chr8_2:30204791-30204810 None:intergenic 40.0%
TCGGTCTTACATTACACAAG+GGG - chr8_2:30205003-30205022 None:intergenic 40.0%
GTCGGTCTTACATTACACAA+GGG - chr8_2:30205004-30205023 None:intergenic 40.0%
! GTTTGTTTAAGGTGGTTGGT+CGG - chr8_2:30205030-30205049 None:intergenic 40.0%
CTGCATGTGTTTGTTTAAGG+TGG - chr8_2:30205038-30205057 None:intergenic 40.0%
GATCTTAGAATTGCCATGGT+TGG - chr8_2:30205215-30205234 None:intergenic 40.0%
GAAGAGATGATGAACGATGA+AGG - chr8_2:30205259-30205278 None:intergenic 40.0%
! CTGTGTACACTGTGTTTTCT+GGG - chr8_2:30205304-30205323 None:intergenic 40.0%
TCTCCATTATTCTCTCTCGC+CGG + chr8_2:30204795-30204814 Msa1243740:CDS 45.0%
! ATACCTGAGTTGAAGAACGC+CGG - chr8_2:30204817-30204836 None:intergenic 45.0%
! TGGGTATGGGTTCGGTATTT+CGG - chr8_2:30204979-30204998 None:intergenic 45.0%
CTTACATTACACAAGGGGGT+GGG - chr8_2:30204998-30205017 None:intergenic 45.0%
TCTTACATTACACAAGGGGG+TGG - chr8_2:30204999-30205018 None:intergenic 45.0%
CGGTCTTACATTACACAAGG+GGG - chr8_2:30205002-30205021 None:intergenic 45.0%
GGTCGGTCTTACATTACACA+AGG - chr8_2:30205005-30205024 None:intergenic 45.0%
TCTCTCTCTCAAACCAACCA+TGG + chr8_2:30205199-30205218 Msa1243740:three_prime_UTR 45.0%
CTGGGAGATTGCTCATGTTA+CGG - chr8_2:30205286-30205305 None:intergenic 45.0%
! CCTGTGTACACTGTGTTTTC+TGG - chr8_2:30205305-30205324 None:intergenic 45.0%
CCAGAAAACACAGTGTACAC+AGG + chr8_2:30205302-30205321 Msa1243740:three_prime_UTR 45.0%
ATGGAGACGAACGGGTGAAA+GGG - chr8_2:30204782-30204801 None:intergenic 50.0%
AATGGAGACGAACGGGTGAA+AGG - chr8_2:30204783-30204802 None:intergenic 50.0%
!! GGTTCGGTATTTCGGGTTTC+GGG - chr8_2:30204971-30204990 None:intergenic 50.0%
!! GGGTTCGGTATTTCGGGTTT+CGG - chr8_2:30204972-30204991 None:intergenic 50.0%
! GGGTATGGGTTCGGTATTTC+GGG - chr8_2:30204978-30204997 None:intergenic 50.0%
TTACACAAGGGGGTGGGTAT+GGG - chr8_2:30204992-30205011 None:intergenic 50.0%
ATTACACAAGGGGGTGGGTA+TGG - chr8_2:30204993-30205012 None:intergenic 50.0%
! GTTTAAGGTGGTTGGTCGGT+CGG - chr8_2:30205026-30205045 None:intergenic 50.0%
AACGGGTGAAAGGGGAGGAT+CGG - chr8_2:30204773-30204792 None:intergenic 55.0%
TGGAGACGAACGGGTGAAAG+GGG - chr8_2:30204781-30204800 None:intergenic 55.0%
ACGCCGGCGAGAGAGAATAA+TGG - chr8_2:30204801-30204820 None:intergenic 55.0%
ACGGGTGAAAGGGGAGGATC+GGG - chr8_2:30204772-30204791 None:intergenic 60.0%
AGACGAACGGGTGAAAGGGG+AGG - chr8_2:30204778-30204797 None:intergenic 60.0%
TCGCCGGCGTTCTTCAACTC+AGG + chr8_2:30204811-30204830 Msa1243740:CDS 60.0%
CAAGGGGGTGGGTATGGGTT+CGG - chr8_2:30204987-30205006 None:intergenic 60.0%
! AAGGTGGTTGGTCGGTCGGT+CGG - chr8_2:30205022-30205041 None:intergenic 60.0%
CGGGTGAAAGGGGAGGATCG+GGG - chr8_2:30204771-30204790 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 30204626 30205331 30204626 ID=Msa1243740;Name=Msa1243740
chr8_2 mRNA 30204626 30205331 30204626 ID=Msa1243740-mRNA-1;Parent=Msa1243740;Name=Msa1243740-mRNA-1;_AED=0.21;_eAED=0.21;_QI=0|0.5|0.33|0.66|0|0|3|320|63
chr8_2 exon 30204626 30204630 30204626 ID=Msa1243740-mRNA-1:exon:10198;Parent=Msa1243740-mRNA-1
chr8_2 exon 30204698 30204832 30204698 ID=Msa1243740-mRNA-1:exon:10199;Parent=Msa1243740-mRNA-1
chr8_2 exon 30204960 30205331 30204960 ID=Msa1243740-mRNA-1:exon:10200;Parent=Msa1243740-mRNA-1
chr8_2 CDS 30204626 30204630 30204626 ID=Msa1243740-mRNA-1:cds;Parent=Msa1243740-mRNA-1
chr8_2 CDS 30204698 30204832 30204698 ID=Msa1243740-mRNA-1:cds;Parent=Msa1243740-mRNA-1
chr8_2 CDS 30204960 30205011 30204960 ID=Msa1243740-mRNA-1:cds;Parent=Msa1243740-mRNA-1
chr8_2 three_prime_UTR 30205012 30205331 30205012 ID=Msa1243740-mRNA-1:three_prime_utr;Parent=Msa1243740-mRNA-1
Gene Sequence

>Msa1243740

ATGAAAGGTTACCACCAGAACAACATTCTAAACAATAATCAAAACACAAATCTTTCCTTGTATCATAGAATTGATCCCCGATCCTCCCCTTTCACCCGTTCGTCTCCATTATTCTCTCTCGCCGGCGTTCTTCAACTCAGTACTGAAGCCCGAAACCCGAAATACCGAACCCATACCCACCCCCTTGTGTAA

Protein sequence

>Msa1243740

MKGYHQNNILNNNQNTNLSLYHRIDPRSSPFTRSSPLFSLAGVLQLSTEARNPKYRTHTHPLV*