AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0434910


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0434910 MtrunA17_Chr3g0141701 87.179 78 8 2 1 77 46 122 2.50e-39 127
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 20 sgRNAs with CRISPR-Local

Find 19 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATACGGTTGCAGCTGGTGC+TGG 0.372363 3_2:+87935795 None:intergenic
TTTGACTTGGACTTGTACTT+TGG 0.409420 3_2:+87935686 None:intergenic
CTCATGTATCTTGTTTGACT+TGG 0.410489 3_2:+87935673 None:intergenic
ATACGGTTGCAGCTGGTGCT+GGG 0.483882 3_2:+87935796 None:intergenic
CAAGTCAGTGGTTGTAGAGC+AGG 0.484666 3_2:-87935826 Msa0434910:CDS
CTGCTCTACAACCACTGACT+TGG 0.511839 3_2:+87935827 None:intergenic
ACCACTGACTTGGCAGGTGG+CGG 0.515445 3_2:+87935837 None:intergenic
TTGTACTTTGGAAGCATCAT+CGG 0.516399 3_2:+87935698 None:intergenic
TCTACAACCACTGACTTGGC+AGG 0.516779 3_2:+87935831 None:intergenic
ATGAAGGTAAGAAGAATGAT+CGG 0.544526 3_2:-87935891 Msa0434910:CDS
TGAAGGTAAGAAGAATGATC+GGG 0.567599 3_2:-87935890 Msa0434910:CDS
TGGCAATGGTAATAATGATA+CGG 0.579053 3_2:+87935779 None:intergenic
AATAATGATACGGTTGCAGC+TGG 0.580071 3_2:+87935789 None:intergenic
ACCGCCACCTGCCAAGTCAG+TGG 0.586261 3_2:-87935838 Msa0434910:CDS
TGATTATGATGCGATTGTGA+TGG 0.609805 3_2:+87935753 None:intergenic
TGATGCGATTGTGATGGAGT+TGG 0.619596 3_2:+87935759 None:intergenic
TACTTTGGAAGCATCATCGG+TGG 0.630715 3_2:+87935701 None:intergenic
ACAACCACTGACTTGGCAGG+TGG 0.640558 3_2:+87935834 None:intergenic
TACGGTTGCAGCTGGTGCTG+GGG 0.642249 3_2:+87935797 None:intergenic
GATTGTGATGGAGTTGGCAA+TGG 0.656251 3_2:+87935765 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
ATGAAGGTAAGAAGAATGAT+CGG - chr3_2:87935674-87935693 Msa0434910:CDS 30.0%
TGGCAATGGTAATAATGATA+CGG + chr3_2:87935789-87935808 None:intergenic 30.0%
TGAAGGTAAGAAGAATGATC+GGG - chr3_2:87935675-87935694 Msa0434910:CDS 35.0%
TGATTATGATGCGATTGTGA+TGG + chr3_2:87935815-87935834 None:intergenic 35.0%
!! TTGTACTTTGGAAGCATCAT+CGG + chr3_2:87935870-87935889 None:intergenic 35.0%
!! TTTGACTTGGACTTGTACTT+TGG + chr3_2:87935882-87935901 None:intergenic 35.0%
AATAATGATACGGTTGCAGC+TGG + chr3_2:87935779-87935798 None:intergenic 40.0%
! GATTGTGATGGAGTTGGCAA+TGG + chr3_2:87935803-87935822 None:intergenic 45.0%
! TGATGCGATTGTGATGGAGT+TGG + chr3_2:87935809-87935828 None:intergenic 45.0%
!! TACTTTGGAAGCATCATCGG+TGG + chr3_2:87935867-87935886 None:intergenic 45.0%
!! TCTACAACCACTGACTTGGC+AGG + chr3_2:87935737-87935756 None:intergenic 50.0%
! CTGCTCTACAACCACTGACT+TGG + chr3_2:87935741-87935760 None:intergenic 50.0%
CAAGTCAGTGGTTGTAGAGC+AGG - chr3_2:87935739-87935758 Msa0434910:CDS 50.0%
!! ACAACCACTGACTTGGCAGG+TGG + chr3_2:87935734-87935753 None:intergenic 55.0%
ATACGGTTGCAGCTGGTGCT+GGG + chr3_2:87935772-87935791 None:intergenic 55.0%
!! ACCACTGACTTGGCAGGTGG+CGG + chr3_2:87935731-87935750 None:intergenic 60.0%
! TACGGTTGCAGCTGGTGCTG+GGG + chr3_2:87935771-87935790 None:intergenic 60.0%
GATACGGTTGCAGCTGGTGC+TGG + chr3_2:87935773-87935792 None:intergenic 60.0%
ACCGCCACCTGCCAAGTCAG+TGG - chr3_2:87935727-87935746 Msa0434910:CDS 65.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 87935674 87935913 87935674 ID=Msa0434910;Name=Msa0434910
chr3_2 mRNA 87935674 87935913 87935674 ID=Msa0434910-mRNA-1;Parent=Msa0434910;Name=Msa0434910-mRNA-1;_AED=0.24;_eAED=0.24;_QI=0|-1|0|1|-1|1|1|0|79
chr3_2 exon 87935674 87935913 87935674 ID=Msa0434910-mRNA-1:exon:25783;Parent=Msa0434910-mRNA-1
chr3_2 CDS 87935674 87935913 87935674 ID=Msa0434910-mRNA-1:cds;Parent=Msa0434910-mRNA-1
Gene Sequence

>Msa0434910

ATGAAGGTAAGAAGAATGATCGGGCACACACAGAGTAACATCATAAGAACAGCACCGCCACCTGCCAAGTCAGTGGTTGTAGAGCAGGAACTTGCCCCAGCACCAGCTGCAACCGTATCATTATTACCATTGCCAACTCCATCACAATCGCATCATAATCACACTGATCTTATAAGCAGCAATCACAATACCACCGATGATGCTTCCAAAGTACAAGTCCAAGTCAAACAAGATACATGA

Protein sequence

>Msa0434910

MKVRRMIGHTQSNIIRTAPPPAKSVVVEQELAPAPAATVSLLPLPTPSQSHHNHTDLISSNHNTTDDASKVQVQVKQDT*