AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0709790


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0709790 MtrunA17_Chr3g0141701 87.179 78 8 2 1 77 46 122 2.50e-39 127
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 20 sgRNAs with CRISPR-Local

Find 19 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATACGGTTGCAGCTGGTGC+TGG 0.372363 5_1:-911626 None:intergenic
TTTGACTTGGACTTGTACTT+TGG 0.409420 5_1:-911735 None:intergenic
CTCATGTATCTTGTTTGACT+TGG 0.410489 5_1:-911748 None:intergenic
ATACGGTTGCAGCTGGTGCT+GGG 0.483882 5_1:-911625 None:intergenic
CAAGTCAGTGGTTGTAGAGC+AGG 0.484666 5_1:+911595 Msa0709790:CDS
CTGCTCTACAACCACTGACT+TGG 0.511839 5_1:-911594 None:intergenic
ACCACTGACTTGGCAGGTGG+CGG 0.515445 5_1:-911584 None:intergenic
TTGTACTTTGGAAGCATCAT+CGG 0.516399 5_1:-911723 None:intergenic
TCTACAACCACTGACTTGGC+AGG 0.516779 5_1:-911590 None:intergenic
ATGAAGGTAAGAAGAATGAT+CGG 0.544526 5_1:+911530 Msa0709790:CDS
TGAAGGTAAGAAGAATGATC+GGG 0.567599 5_1:+911531 Msa0709790:CDS
TGGCAATGGTAATAATGATA+CGG 0.579053 5_1:-911642 None:intergenic
AATAATGATACGGTTGCAGC+TGG 0.580071 5_1:-911632 None:intergenic
ACCGCCACCTGCCAAGTCAG+TGG 0.586261 5_1:+911583 Msa0709790:CDS
TGATTATGATGCGATTGTGA+TGG 0.609805 5_1:-911668 None:intergenic
TGATGCGATTGTGATGGAGT+TGG 0.619596 5_1:-911662 None:intergenic
TACTTTGGAAGCATCATCGG+TGG 0.630715 5_1:-911720 None:intergenic
ACAACCACTGACTTGGCAGG+TGG 0.640558 5_1:-911587 None:intergenic
TACGGTTGCAGCTGGTGCTG+GGG 0.642249 5_1:-911624 None:intergenic
GATTGTGATGGAGTTGGCAA+TGG 0.656251 5_1:-911656 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
ATGAAGGTAAGAAGAATGAT+CGG + chr5_1:911530-911549 Msa0709790:CDS 30.0%
TGGCAATGGTAATAATGATA+CGG - chr5_1:911645-911664 None:intergenic 30.0%
TGAAGGTAAGAAGAATGATC+GGG + chr5_1:911531-911550 Msa0709790:CDS 35.0%
TGATTATGATGCGATTGTGA+TGG - chr5_1:911671-911690 None:intergenic 35.0%
!! TTGTACTTTGGAAGCATCAT+CGG - chr5_1:911726-911745 None:intergenic 35.0%
!! TTTGACTTGGACTTGTACTT+TGG - chr5_1:911738-911757 None:intergenic 35.0%
AATAATGATACGGTTGCAGC+TGG - chr5_1:911635-911654 None:intergenic 40.0%
! GATTGTGATGGAGTTGGCAA+TGG - chr5_1:911659-911678 None:intergenic 45.0%
! TGATGCGATTGTGATGGAGT+TGG - chr5_1:911665-911684 None:intergenic 45.0%
!! TACTTTGGAAGCATCATCGG+TGG - chr5_1:911723-911742 None:intergenic 45.0%
!! TCTACAACCACTGACTTGGC+AGG - chr5_1:911593-911612 None:intergenic 50.0%
! CTGCTCTACAACCACTGACT+TGG - chr5_1:911597-911616 None:intergenic 50.0%
CAAGTCAGTGGTTGTAGAGC+AGG + chr5_1:911595-911614 Msa0709790:CDS 50.0%
!! ACAACCACTGACTTGGCAGG+TGG - chr5_1:911590-911609 None:intergenic 55.0%
ATACGGTTGCAGCTGGTGCT+GGG - chr5_1:911628-911647 None:intergenic 55.0%
!! ACCACTGACTTGGCAGGTGG+CGG - chr5_1:911587-911606 None:intergenic 60.0%
! TACGGTTGCAGCTGGTGCTG+GGG - chr5_1:911627-911646 None:intergenic 60.0%
GATACGGTTGCAGCTGGTGC+TGG - chr5_1:911629-911648 None:intergenic 60.0%
ACCGCCACCTGCCAAGTCAG+TGG + chr5_1:911583-911602 Msa0709790:CDS 65.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 911530 911769 911530 ID=Msa0709790;Name=Msa0709790
chr5_1 mRNA 911530 911769 911530 ID=Msa0709790-mRNA-1;Parent=Msa0709790;Name=Msa0709790-mRNA-1;_AED=0.28;_eAED=0.28;_QI=0|-1|0|1|-1|1|1|0|79
chr5_1 exon 911530 911769 911530 ID=Msa0709790-mRNA-1:exon:458;Parent=Msa0709790-mRNA-1
chr5_1 CDS 911530 911769 911530 ID=Msa0709790-mRNA-1:cds;Parent=Msa0709790-mRNA-1
Gene Sequence

>Msa0709790

ATGAAGGTAAGAAGAATGATCGGGCACACACAGAGTAACATCATAAGAACAGCACCGCCACCTGCCAAGTCAGTGGTTGTAGAGCAGGAACTTGCCCCAGCACCAGCTGCAACCGTATCATTATTACCATTGCCAACTCCATCACAATCGCATCATAATCACACTGATCTTATAAGCAGCAATCACAATACCACCGATGATGCTTCCAAAGTACAAGTCCAAGTCAAACAAGATACATGA

Protein sequence

>Msa0709790

MKVRRMIGHTQSNIIRTAPPPAKSVVVEQELAPAPAATVSLLPLPTPSQSHHNHTDLISSNHNTTDDASKVQVQVKQDT*