AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0947550

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0947550 MtrunA17_Chr6g0462031 97.934 242 2 1 1 239 1 242 7.24e-176 483
Msa0947550 MtrunA17_Chr4g0006181 37.743 257 109 6 22 231 88 340 1.88e-44 152
Msa0947550 MtrunA17_Chr3g0092091 37.970 266 117 10 1 235 1 249 2.03e-42 144
Msa0947550 MtrunA17_Chr8g0345631 40.758 211 97 7 46 237 66 267 8.42e-42 143
Msa0947550 MtrunA17_Chr3g0133901 38.565 223 105 7 33 235 52 262 4.01e-41 141
Msa0947550 MtrunA17_Chr2g0301081 40.976 205 93 7 46 231 65 260 1.91e-40 139
Msa0947550 MtrunA17_Chr6g0476641 38.743 191 107 3 53 235 91 279 2.07e-37 132
Msa0947550 MtrunA17_Chr6g0453181 42.408 191 94 6 53 230 66 253 1.93e-36 129
Msa0947550 MtrunA17_Chr5g0448411 37.959 245 116 9 16 231 31 268 1.96e-36 129
Msa0947550 MtrunA17_Chr6g0453151 42.487 193 91 6 53 230 133 320 2.36e-36 130
Msa0947550 MtrunA17_Chr4g0051941 43.147 197 91 7 49 230 64 254 2.91e-36 129
Msa0947550 MtrunA17_Chr6g0453141 42.487 193 91 6 53 230 66 253 3.33e-36 129
Msa0947550 MtrunA17_Chr5g0448421 38.710 248 112 10 16 231 31 270 3.99e-35 126
Msa0947550 MtrunA17_Chr5g0447531 39.474 228 100 9 36 230 31 253 7.61e-35 125
Msa0947550 MtrunA17_Chr6g0453161 42.487 193 91 6 53 230 68 255 2.87e-34 128
Msa0947550 MtrunA17_Chr6g0453161 41.969 193 92 6 53 230 333 520 1.28e-33 127
Msa0947550 MtrunA17_Chr1g0157481 39.000 200 100 6 53 231 125 323 7.45e-34 124
Msa0947550 MtrunA17_Chr3g0113311 34.545 220 96 5 46 221 60 275 6.08e-31 115
Msa0947550 MtrunA17_Chr2g0318521 37.104 221 110 9 36 230 35 252 5.48e-30 112
Msa0947550 MtrunA17_Chr6g0464511 38.202 178 86 6 53 230 47 200 5.12e-23 92.8
Msa0947550 MtrunA17_Chr2g0279001 33.846 195 79 6 46 201 60 243 2.62e-21 89.4
Msa0947550 MtrunA17_Chr6g0453171 56.061 66 28 1 165 230 22 86 6.22e-19 79.0
Msa0947550 MtrunA17_Chr2g0296081 46.479 71 33 2 165 235 36 101 1.54e-12 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0947550 AT3G54890.1 82.845 239 39 2 1 237 1 239 7.12e-140 392
Msa0947550 AT3G54890.4 81.122 196 35 2 1 194 1 196 7.32e-108 310
Msa0947550 AT3G54890.2 69.456 239 37 3 1 237 1 205 1.64e-106 306
Msa0947550 AT3G54890.3 74.684 158 37 3 1 155 1 158 1.30e-72 219
Msa0947550 AT3G08940.2 38.113 265 111 7 16 231 20 280 1.03e-46 157
Msa0947550 AT3G47470.1 37.452 259 121 8 1 231 1 246 1.30e-44 150
Msa0947550 AT5G01530.1 39.496 238 93 6 41 231 50 283 2.56e-44 150
Msa0947550 AT3G61470.1 40.284 211 98 7 46 237 55 256 2.59e-40 139
Msa0947550 AT1G45474.2 37.327 217 101 5 44 236 47 252 2.87e-40 139
Msa0947550 AT1G45474.1 37.327 217 101 5 44 236 47 252 2.87e-40 139
Msa0947550 AT2G34430.1 44.103 195 93 6 49 230 63 254 1.48e-38 135
Msa0947550 AT2G34420.1 44.103 195 93 6 49 230 62 253 1.63e-38 135
Msa0947550 AT2G40100.1 38.356 219 88 4 46 221 59 273 3.37e-38 134
Msa0947550 AT4G10340.1 39.791 191 105 3 53 235 82 270 3.63e-38 134
Msa0947550 AT1G29920.1 43.455 191 93 5 53 230 67 255 3.81e-38 134
Msa0947550 AT1G29910.1 43.455 191 93 5 53 230 67 255 3.81e-38 134
Msa0947550 AT1G29930.1 43.455 191 93 5 53 230 67 255 4.19e-38 134
Msa0947550 AT1G19150.1 39.512 205 96 7 46 231 68 263 7.69e-38 133
Msa0947550 AT2G05100.1 41.451 193 93 6 53 230 66 253 3.91e-35 126
Msa0947550 AT2G05070.1 41.451 193 93 6 53 230 66 253 4.16e-35 126
Msa0947550 AT3G27690.2 40.933 193 94 6 53 230 99 286 3.24e-34 124
Msa0947550 AT3G27690.1 40.933 193 94 6 53 230 67 254 3.89e-34 124
Msa0947550 AT1G61520.3 40.465 215 95 9 46 231 56 266 1.45e-33 122
Msa0947550 AT1G61520.1 40.465 215 95 9 46 231 56 266 1.45e-33 122
Msa0947550 AT1G76570.1 37.000 200 104 6 53 231 123 321 1.82e-32 120
Msa0947550 AT5G54270.1 34.766 256 138 8 1 230 1 253 5.50e-32 118
Msa0947550 AT1G61520.2 40.594 202 87 9 59 231 14 211 4.49e-30 112
Msa0947550 AT2G05100.2 39.645 169 83 5 53 206 66 230 1.03e-26 105
Msa0947550 AT1G15820.1 35.165 182 74 6 53 201 70 240 2.29e-20 87.4
Msa0947550 AT5G28450.1 53.333 75 32 3 165 237 99 172 1.98e-15 72.0

Find 90 sgRNAs with CRISPR-Local

Find 186 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACTGGACCTTTGGAGAATT+TGG 0.160780 6_3:-30463115 Msa0947550:CDS
ACCCAATGGAAATCCAATTT+TGG 0.171044 6_3:-30465527 Msa0947550:five_prime_UTR
ATGCATGCAGCCAGGGATTT+TGG 0.202155 6_3:-30464001 Msa0947550:intron
CATGACAAAATAAATGTATT+TGG 0.209101 6_3:+30462977 None:intergenic
GATATTGGAAAATCCAAAAT+TGG 0.226960 6_3:+30465514 None:intergenic
GGGCACCCTACCCACCATTT+TGG 0.282770 6_3:-30463881 Msa0947550:CDS
TAGGAGAATGTTAGGGATAT+TGG 0.283556 6_3:+30465499 None:intergenic
TGGATTTGATCCTCTACGTC+TGG 0.312419 6_3:-30464553 Msa0947550:CDS
GTGTAAGTTTGCTTCTATAA+AGG 0.313630 6_3:+30462826 None:intergenic
CCTAGACCACCTTACCTTGA+TGG 0.314069 6_3:-30465231 Msa0947550:CDS
AATGAATATAGGAGAATGTT+AGG 0.315337 6_3:+30465491 None:intergenic
TTCTCCAAAGGTCCAGTTCC+TGG 0.317892 6_3:+30463120 None:intergenic
CAGGAATGGGCAGCACTTCC+TGG 0.319362 6_3:-30463940 Msa0947550:CDS
GTACCAGAGGCATTGGGATT+AGG 0.338005 6_3:-30463979 Msa0947550:CDS
TGTTGAGCACCAAAGGAGTA+TGG 0.346828 6_3:-30463833 Msa0947550:CDS
ATTAGGACGTCTTGCTTTGT+TGG 0.354009 6_3:-30463178 Msa0947550:intron
GGTCTTTCTCCATACTCCTT+TGG 0.357030 6_3:+30463824 None:intergenic
TACCTTGATGGTTCAGCTCC+AGG 0.367208 6_3:-30465219 Msa0947550:CDS
AGGCATTGGGATTAGGCAAC+TGG 0.374324 6_3:-30463972 Msa0947550:CDS
ATATCTATTGAGGTGGAATA+AGG 0.375187 6_3:+30463049 None:intergenic
AAGGAATTCAATGACCAAAA+TGG 0.388111 6_3:+30463867 None:intergenic
TATAACAAAGATTGATTTGA+GGG 0.395396 6_3:+30463024 None:intergenic
TCCAAAATTGGATTTCCATT+GGG 0.399822 6_3:+30465526 None:intergenic
AGGTAGGTTGCTTGTCCTCC+AGG 0.409848 6_3:+30463922 None:intergenic
GATTCACCATGACTTCTGAT+TGG 0.414343 6_3:-30465266 Msa0947550:CDS
ATCCAAAATTGGATTTCCAT+TGG 0.416315 6_3:+30465525 None:intergenic
TCATGAATCAGATCTCTCTA+TGG 0.424717 6_3:-30462959 Msa0947550:three_prime_UTR
ACATAAGAGTGAATGAATAT+AGG 0.430012 6_3:+30465480 None:intergenic
GATAATATTAAGATACCTCT+TGG 0.432597 6_3:-30462906 Msa0947550:three_prime_UTR
CCATCAAGGTAAGGTGGTCT+AGG 0.434279 6_3:+30465231 None:intergenic
TTGAATCTCTCAAGATTCTC+AGG 0.437523 6_3:+30464522 None:intergenic
TCAAATTTAATTACAAAGAC+AGG 0.441517 6_3:+30462927 None:intergenic
AAGGAGTTAAGATATATCTA+TGG 0.457353 6_3:-30462878 Msa0947550:three_prime_UTR
ATGACTTCTGATTGGATGCC+TGG 0.463135 6_3:-30465258 Msa0947550:CDS
ATATCCAGGAACTGGACCTT+TGG 0.468791 6_3:-30463124 Msa0947550:CDS
CTGAACTCATTCACTGCAGA+TGG 0.472063 6_3:-30464494 Msa0947550:CDS
GAAGCATTTGTGCAAGGAAG+TGG 0.473273 6_3:+30465294 None:intergenic
CCTAGGCAACCCTGTTCCAT+GGG 0.474387 6_3:-30463902 Msa0947550:CDS
ACCTAGGCAACCCTGTTCCA+TGG 0.483827 6_3:-30463903 Msa0947550:CDS
CTGGGTGAAAGCACAGGAAT+GGG 0.485329 6_3:-30463953 Msa0947550:CDS
GGAGAATTTGGCAACACACT+TGG 0.485817 6_3:-30463103 Msa0947550:CDS
ACCTTGATGGTTCAGCTCCA+GGG 0.491528 6_3:-30465218 Msa0947550:intron
CAAACAGCATATCCAGGAAC+TGG 0.497647 6_3:-30463132 Msa0947550:CDS
GGATTTGATCCTCTACGTCT+GGG 0.503278 6_3:-30464552 Msa0947550:CDS
GCTCAACAAAAGCAATAGAA+AGG 0.508788 6_3:+30463848 None:intergenic
GCCTCTGGTACCAAAATCCC+TGG 0.509037 6_3:+30463991 None:intergenic
GACAAAATAAATGTATTTGG+TGG 0.511225 6_3:+30462980 None:intergenic
TTGCCTAATCCCAATGCCTC+TGG 0.512790 6_3:+30463976 None:intergenic
AGGCAACTGGGTGAAAGCAC+AGG 0.515063 6_3:-30463959 Msa0947550:CDS
TTTGAGGGAGATATCTATTG+AGG 0.515176 6_3:+30463039 None:intergenic
ACTGGGTGAAAGCACAGGAA+TGG 0.517047 6_3:-30463954 Msa0947550:CDS
TTTGAGGAAGAAAGAAGTGA+TGG 0.520641 6_3:+30465339 None:intergenic
TTTCCAATGTTGTTGTGCCA+TGG 0.523601 6_3:+30463075 None:intergenic
CAATGACCAAAATGGTGGGT+AGG 0.530752 6_3:+30463875 None:intergenic
GATCCATGGCACAACAACAT+TGG 0.533754 6_3:-30463078 Msa0947550:CDS
AAAATCAAAGAAGTTAAGAA+TGG 0.539315 6_3:-30463724 Msa0947550:intron
AATGACCAAAATGGTGGGTA+GGG 0.541785 6_3:+30463876 None:intergenic
ATATAACAAAGATTGATTTG+AGG 0.556606 6_3:+30463023 None:intergenic
CAACACACTTGGCTGATCCA+TGG 0.557652 6_3:-30463092 Msa0947550:CDS
TTGTGTTTGTGTGACAAGAA+TGG 0.559021 6_3:-30465400 Msa0947550:exon
CCCATGGAACAGGGTTGCCT+AGG 0.565700 6_3:+30463902 None:intergenic
GTAGGGTGCCCCATGGAACA+GGG 0.567362 6_3:+30463893 None:intergenic
TAAGGTGGTCTAGGCTGACC+AGG 0.567668 6_3:+30465240 None:intergenic
AAGATACCTCTTGGCATCAA+AGG 0.570679 6_3:-30462897 Msa0947550:three_prime_UTR
GGAGGACAAGCAACCTACCT+AGG 0.572729 6_3:-30463919 Msa0947550:CDS
AAAGGATCAAAAGCACCACC+AGG 0.573417 6_3:+30463784 None:intergenic
GAATTCAATGACCAAAATGG+TGG 0.574206 6_3:+30463870 None:intergenic
TGGAACAGGGTTGCCTAGGT+AGG 0.576958 6_3:+30463906 None:intergenic
GATACTTACAACAGCAAGCA+TGG 0.578696 6_3:+30464470 None:intergenic
AGGCATCCAATCAGAAGTCA+TGG 0.583801 6_3:+30465260 None:intergenic
ATGAATATAGGAGAATGTTA+GGG 0.584903 6_3:+30465492 None:intergenic
GGAGCTGAACCATCAAGGTA+AGG 0.587136 6_3:+30465222 None:intergenic
TGAACTCATTCACTGCAGAT+GGG 0.591140 6_3:-30464493 Msa0947550:CDS
ACCCTGGAGCTGAACCATCA+AGG 0.592237 6_3:+30465217 None:intergenic
GAGGGAGATATCTATTGAGG+TGG 0.592911 6_3:+30463042 None:intergenic
GTTCAACAAACAGCATATCC+AGG 0.600057 6_3:-30463138 Msa0947550:CDS
GGCATTGGGATTAGGCAACT+GGG 0.602759 6_3:-30463971 Msa0947550:CDS
TTAACTCCTTTGATGCCAAG+AGG 0.615697 6_3:+30462891 None:intergenic
CTAGAAGAAGCATTTGTGCA+AGG 0.615959 6_3:+30465288 None:intergenic
TGTGTTGCCAAATTCTCCAA+AGG 0.630431 6_3:+30463108 None:intergenic
AATTCAATGACCAAAATGGT+GGG 0.634135 6_3:+30463871 None:intergenic
GGGTCTTTAGAGTAACCCAA+AGG 0.636573 6_3:+30463766 None:intergenic
ATGGTGGGTAGGGTGCCCCA+TGG 0.643524 6_3:+30463886 None:intergenic
GCTGAACCATCAAGGTAAGG+TGG 0.647312 6_3:+30465225 None:intergenic
GGTACTTCACCCAGACGTAG+AGG 0.653463 6_3:+30464543 None:intergenic
CCTGAAAAGAAGAAGTACCC+TGG 0.684859 6_3:-30463802 Msa0947550:CDS
CTAGGCAACCCTGTTCCATG+GGG 0.718085 6_3:-30463901 Msa0947550:CDS
AAGGATCAAAAGCACCACCA+GGG 0.718614 6_3:+30463785 None:intergenic
GAATGGGCAGCACTTCCTGG+AGG 0.731277 6_3:-30463937 Msa0947550:CDS
GAAAAGAAGAAGTACCCTGG+TGG 0.742886 6_3:-30463799 Msa0947550:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTTTACCATTATTTTTTT+TGG - chr6_3:30463149-30463168 Msa0947550:CDS 10.0%
!! ATATTTACCAAAAAAAATAA+TGG + chr6_3:30463159-30463178 None:intergenic 10.0%
!!! TACAAAATAATTTTTTTAAG+AGG - chr6_3:30463665-30463684 Msa0947550:intron 10.0%
!! TAACTAAATAAAGGTTTATA+AGG + chr6_3:30463394-30463413 None:intergenic 15.0%
!!! CAATTTTCTTAACTAAATAA+AGG + chr6_3:30463403-30463422 None:intergenic 15.0%
!!! ATAATTTTTTTAAGAGGTAA+AGG - chr6_3:30463671-30463690 Msa0947550:intron 15.0%
!!! TTAATAACGTTCAAATTTTT+TGG + chr6_3:30464811-30464830 None:intergenic 15.0%
!!! ACATTTCTGAAAATATTATA+TGG - chr6_3:30465044-30465063 Msa0947550:intron 15.0%
!!! TAGTTTTCAATATCGATATT+AGG - chr6_3:30464051-30464070 Msa0947550:intron 20.0%
!!! TGTATTCATGAAACTTTTTA+GGG + chr6_3:30464581-30464600 None:intergenic 20.0%
!!! TTGTATTCATGAAACTTTTT+AGG + chr6_3:30464582-30464601 None:intergenic 20.0%
!! AAAATCAAAGAAGTTAAGAA+TGG - chr6_3:30464600-30464619 Msa0947550:intron 20.0%
!!! TATAACAAAGATTGATTTGA+GGG + chr6_3:30465303-30465322 None:intergenic 20.0%
!!! ATATAACAAAGATTGATTTG+AGG + chr6_3:30465304-30465323 None:intergenic 20.0%
!! CATGACAAAATAAATGTATT+TGG + chr6_3:30465350-30465369 None:intergenic 20.0%
!! TCAAATTTAATTACAAAGAC+AGG + chr6_3:30465400-30465419 None:intergenic 20.0%
! GATATTGGAAAATCCAAAAT+TGG + chr6_3:30462813-30462832 None:intergenic 25.0%
! ATGAATATAGGAGAATGTTA+GGG + chr6_3:30462835-30462854 None:intergenic 25.0%
! AATGAATATAGGAGAATGTT+AGG + chr6_3:30462836-30462855 None:intergenic 25.0%
! ACATAAGAGTGAATGAATAT+AGG + chr6_3:30462847-30462866 None:intergenic 25.0%
!! AGTTGAAAATCTTGATTTTG+AGG + chr6_3:30463004-30463023 None:intergenic 25.0%
!! TAATGGACAAACCTAAATTT+AGG - chr6_3:30463324-30463343 Msa0947550:intron 25.0%
!! TAGTGTATGAATATTTGTTG+TGG - chr6_3:30463727-30463746 Msa0947550:CDS 25.0%
!!! TTTTTTTGTTACAGTGACTT+TGG - chr6_3:30463751-30463770 Msa0947550:CDS 25.0%
!! GTTGTAAGTATCTCTTTTAT+AGG - chr6_3:30463865-30463884 Msa0947550:CDS 25.0%
!! TTTTATAGGAAAACCAGTTT+AGG - chr6_3:30463879-30463898 Msa0947550:CDS 25.0%
! TTTCAATATCGATATTAGGT+AGG - chr6_3:30464055-30464074 Msa0947550:intron 25.0%
! ACGTTATTAATCACAATTTC+AGG - chr6_3:30464821-30464840 Msa0947550:intron 25.0%
! ATTTGAATACTTGAAACTCA+TGG - chr6_3:30464862-30464881 Msa0947550:intron 25.0%
! TAAACTCGACAACATTTAAA+TGG + chr6_3:30464999-30465018 None:intergenic 25.0%
! CTTTGTTGAATGTTTGTATT+AGG - chr6_3:30465129-30465148 Msa0947550:intron 25.0%
! GACAAAATAAATGTATTTGG+TGG + chr6_3:30465347-30465366 None:intergenic 25.0%
! GATAATATTAAGATACCTCT+TGG - chr6_3:30465418-30465437 Msa0947550:five_prime_UTR 25.0%
! AAGGAGTTAAGATATATCTA+TGG - chr6_3:30465446-30465465 Msa0947550:five_prime_UTR 25.0%
ATCCAAAATTGGATTTCCAT+TGG + chr6_3:30462802-30462821 None:intergenic 30.0%
! TGTCTAGTGTCTACTATTTT+TGG + chr6_3:30462903-30462922 None:intergenic 30.0%
CATGTGTGACAATATTTAAG+AGG + chr6_3:30463281-30463300 None:intergenic 30.0%
AACCTAAATTTAGGTGTGTT+TGG - chr6_3:30463333-30463352 Msa0947550:intron 30.0%
ATCCAAACACACCTAAATTT+AGG + chr6_3:30463338-30463357 None:intergenic 30.0%
GTTAAGAAAATTGAAGTCGT+CGG - chr6_3:30463410-30463429 Msa0947550:intron 30.0%
TGTGATTGTTCGATCAAAAT+CGG - chr6_3:30463463-30463482 Msa0947550:intron 30.0%
!!! CAGTGTATACTAGTTTTACA+AGG - chr6_3:30463999-30464018 Msa0947550:intron 30.0%
TGAAAACTACTATGAAGTAC+TGG + chr6_3:30464040-30464059 None:intergenic 30.0%
TCGATATTAGGTAGGTTTAA+TGG - chr6_3:30464063-30464082 Msa0947550:intron 30.0%
TTAGGTAGGTTTAATGGAAT+CGG - chr6_3:30464069-30464088 Msa0947550:intron 30.0%
! CAGTGTAAAACTATTTTACG+CGG - chr6_3:30464261-30464280 Msa0947550:intron 30.0%
AATTCAATGACCAAAATGGT+GGG + chr6_3:30464456-30464475 None:intergenic 30.0%
AAGGAATTCAATGACCAAAA+TGG + chr6_3:30464460-30464479 None:intergenic 30.0%
ATATCTATTGAGGTGGAATA+AGG + chr6_3:30465278-30465297 None:intergenic 30.0%
! GTGTAAGTTTGCTTCTATAA+AGG + chr6_3:30465501-30465520 None:intergenic 30.0%
TAGGAGAATGTTAGGGATAT+TGG + chr6_3:30462828-30462847 None:intergenic 35.0%
TTGTGTTTGTGTGACAAGAA+TGG - chr6_3:30462924-30462943 Msa0947550:three_prime_UTR 35.0%
TTTGAGGAAGAAAGAAGTGA+TGG + chr6_3:30462988-30463007 None:intergenic 35.0%
GCATTAGTATATACATACCC+TGG + chr6_3:30463126-30463145 None:intergenic 35.0%
GAGTTGAATTGTTGTAGTAG+TGG - chr6_3:30463205-30463224 Msa0947550:intron 35.0%
! CGATCAAAATCGGACAATTT+TGG - chr6_3:30463473-30463492 Msa0947550:intron 35.0%
ACCTCTCCTCATATTAATTG+AGG - chr6_3:30463626-30463645 Msa0947550:intron 35.0%
CCTCTCCTCATATTAATTGA+GGG - chr6_3:30463627-30463646 Msa0947550:intron 35.0%
CTCTCCTCATATTAATTGAG+GGG - chr6_3:30463628-30463647 Msa0947550:intron 35.0%
TCTCCTCATATTAATTGAGG+GGG - chr6_3:30463629-30463648 Msa0947550:intron 35.0%
TTGAATCTCTCAAGATTCTC+AGG + chr6_3:30463805-30463824 None:intergenic 35.0%
! AAACCAGTTTAGGTTTCTCT+TGG - chr6_3:30463889-30463908 Msa0947550:CDS 35.0%
ATCAAACGGTCTAGATCAAA+CGG - chr6_3:30464116-30464135 Msa0947550:intron 35.0%
CAAACGGTCTAGATTTCAAA+CGG + chr6_3:30464165-30464184 None:intergenic 35.0%
TGTAAACAGTCAATGGTTGT+TGG + chr6_3:30464196-30464215 None:intergenic 35.0%
TTATATTATGCATGCAGCCA+GGG - chr6_3:30464316-30464335 Msa0947550:intron 35.0%
GAATTCAATGACCAAAATGG+TGG + chr6_3:30464457-30464476 None:intergenic 35.0%
GCTCAACAAAAGCAATAGAA+AGG + chr6_3:30464479-30464498 None:intergenic 35.0%
GTTAACGCAGTTTACATGTA+TGG + chr6_3:30464977-30464996 None:intergenic 35.0%
TTTGAGGGAGATATCTATTG+AGG + chr6_3:30465288-30465307 None:intergenic 35.0%
TCATGAATCAGATCTCTCTA+TGG - chr6_3:30465365-30465384 Msa0947550:CDS 35.0%
!! CTAGAAGAAGCATTTGTGCA+AGG + chr6_3:30463039-30463058 None:intergenic 40.0%
GATTCACCATGACTTCTGAT+TGG - chr6_3:30463058-30463077 Msa0947550:CDS 40.0%
CGGAAATTCTCGCATTTATG+CGG - chr6_3:30463430-30463449 Msa0947550:intron 40.0%
AATTCTCGCATTTATGCGGT+CGG - chr6_3:30463434-30463453 Msa0947550:intron 40.0%
ACCGTTTGATCTTGATCCAA+CGG - chr6_3:30463549-30463568 Msa0947550:intron 40.0%
ACCGTTGGATCAAGATCAAA+CGG + chr6_3:30463553-30463572 None:intergenic 40.0%
! ATTTTCCGCATTCATGCAGT+CGG + chr6_3:30463586-30463605 None:intergenic 40.0%
CCCTCAATTAATATGAGGAG+AGG + chr6_3:30463630-30463649 None:intergenic 40.0%
TGAACTCATTCACTGCAGAT+GGG - chr6_3:30463831-30463850 Msa0947550:CDS 40.0%
GATACTTACAACAGCAAGCA+TGG + chr6_3:30463857-30463876 None:intergenic 40.0%
ATGCCAAGAGAAACCTAAAC+TGG + chr6_3:30463895-30463914 None:intergenic 40.0%
!! ACCGTCCGGTTTTGATATAA+CGG - chr6_3:30463934-30463953 Msa0947550:CDS 40.0%
ACCGTTATATCAAAACCGGA+CGG + chr6_3:30463938-30463957 None:intergenic 40.0%
CCGAACCGTTATATCAAAAC+CGG + chr6_3:30463942-30463961 None:intergenic 40.0%
!! CCGGTTTTGATATAACGGTT+CGG - chr6_3:30463939-30463958 Msa0947550:CDS 40.0%
TCGATCCAACAGACATTGAT+GGG + chr6_3:30464095-30464114 None:intergenic 40.0%
TCTGTTGGATCGAGATCAAA+CGG - chr6_3:30464102-30464121 Msa0947550:intron 40.0%
TTTCAAACGGTCCATCAGAT+GGG + chr6_3:30464152-30464171 None:intergenic 40.0%
ATTTCAAACGGTCCATCAGA+TGG + chr6_3:30464153-30464172 None:intergenic 40.0%
GTTGTTGGATCGAGATCAAA+CGG + chr6_3:30464181-30464200 None:intergenic 40.0%
AGGTAGGTGTAAACAGTCAA+TGG + chr6_3:30464203-30464222 None:intergenic 40.0%
TTGACTGTTTACACCTACCT+AGG - chr6_3:30464203-30464222 Msa0947550:intron 40.0%
TACACCTACCTAGGTTTATG+TGG - chr6_3:30464212-30464231 Msa0947550:intron 40.0%
ATTGCCACATAAACCTAGGT+AGG + chr6_3:30464219-30464238 None:intergenic 40.0%
TCACATTGCCACATAAACCT+AGG + chr6_3:30464223-30464242 None:intergenic 40.0%
GTTATATTATGCATGCAGCC+AGG - chr6_3:30464315-30464334 Msa0947550:intron 40.0%
!!! ATTTTGGTACCAGAGGCATT+GGG - chr6_3:30464339-30464358 Msa0947550:intron 40.0%
!! AATGACCAAAATGGTGGGTA+GGG + chr6_3:30464451-30464470 None:intergenic 40.0%
!! TTGCTTTTGTTGAGCACCAA+AGG - chr6_3:30464484-30464503 Msa0947550:CDS 40.0%
!! CAGGGTACTTCTTCTTTTCA+GGG + chr6_3:30464524-30464543 None:intergenic 40.0%
TGTTGTCGAATTTCAGTGCA+CGG + chr6_3:30464695-30464714 None:intergenic 40.0%
ACAGTGCAATGAGTGGTTAT+TGG - chr6_3:30464752-30464771 Msa0947550:intron 40.0%
GTTAATCGCGTTCAAATTCG+TGG - chr6_3:30464884-30464903 Msa0947550:intron 40.0%
GGTTAATGAGATGTTCGATC+TGG + chr6_3:30464952-30464971 None:intergenic 40.0%
ACGCAGTTTACATGTATGGT+TGG + chr6_3:30464973-30464992 None:intergenic 40.0%
ATTAGGACGTCTTGCTTTGT+TGG - chr6_3:30465146-30465165 Msa0947550:intron 40.0%
!!! CTTGCTTTGTTGGCTTTTGT+TGG - chr6_3:30465156-30465175 Msa0947550:intron 40.0%
GTTCAACAAACAGCATATCC+AGG - chr6_3:30465186-30465205 Msa0947550:intron 40.0%
! AACTGGACCTTTGGAGAATT+TGG - chr6_3:30465209-30465228 Msa0947550:intron 40.0%
TGTGTTGCCAAATTCTCCAA+AGG + chr6_3:30465219-30465238 None:intergenic 40.0%
TTTCCAATGTTGTTGTGCCA+TGG + chr6_3:30465252-30465271 None:intergenic 40.0%
AAGATACCTCTTGGCATCAA+AGG - chr6_3:30465427-30465446 Msa0947550:five_prime_UTR 40.0%
TTAACTCCTTTGATGCCAAG+AGG + chr6_3:30465436-30465455 None:intergenic 40.0%
! GAAGCATTTGTGCAAGGAAG+TGG + chr6_3:30463033-30463052 None:intergenic 45.0%
AGGCATCCAATCAGAAGTCA+TGG + chr6_3:30463067-30463086 None:intergenic 45.0%
ATGACTTCTGATTGGATGCC+TGG - chr6_3:30463066-30463085 Msa0947550:CDS 45.0%
CATGTGTCGAATGTCGAACA+CGG - chr6_3:30463364-30463383 Msa0947550:intron 45.0%
TTGATCCAACGGTCACTGAT+GGG - chr6_3:30463560-30463579 Msa0947550:intron 45.0%
GCTCCCCCTCAATTAATATG+AGG + chr6_3:30463635-30463654 None:intergenic 45.0%
TGGATTTGATCCTCTACGTC+TGG - chr6_3:30463771-30463790 Msa0947550:CDS 45.0%
GGATTTGATCCTCTACGTCT+GGG - chr6_3:30463772-30463791 Msa0947550:CDS 45.0%
CTGAACTCATTCACTGCAGA+TGG - chr6_3:30463830-30463849 Msa0947550:CDS 45.0%
CTAGTAGATGTGAAACCGTC+CGG - chr6_3:30463920-30463939 Msa0947550:CDS 45.0%
CTCGATCCAACAGACATTGA+TGG + chr6_3:30464096-30464115 None:intergenic 45.0%
GTCTAAATCAGCCCATCTGA+TGG - chr6_3:30464138-30464157 Msa0947550:intron 45.0%
!! GATTTTGGTACCAGAGGCAT+TGG - chr6_3:30464338-30464357 Msa0947550:intron 45.0%
!! CAATGACCAAAATGGTGGGT+AGG + chr6_3:30464452-30464471 None:intergenic 45.0%
TGTTGAGCACCAAAGGAGTA+TGG - chr6_3:30464491-30464510 Msa0947550:CDS 45.0%
GGTCTTTCTCCATACTCCTT+TGG + chr6_3:30464503-30464522 None:intergenic 45.0%
!! CCAGGGTACTTCTTCTTTTC+AGG + chr6_3:30464525-30464544 None:intergenic 45.0%
!! CCTGAAAAGAAGAAGTACCC+TGG - chr6_3:30464522-30464541 Msa0947550:CDS 45.0%
!! GAAAAGAAGAAGTACCCTGG+TGG - chr6_3:30464525-30464544 Msa0947550:CDS 45.0%
!! AAGGATCAAAAGCACCACCA+GGG + chr6_3:30464542-30464561 None:intergenic 45.0%
!! AAAGGATCAAAAGCACCACC+AGG + chr6_3:30464543-30464562 None:intergenic 45.0%
!! TGGTGGTGCTTTTGATCCTT+TGG - chr6_3:30464542-30464561 Msa0947550:CDS 45.0%
! GGTGGTGCTTTTGATCCTTT+GGG - chr6_3:30464543-30464562 Msa0947550:CDS 45.0%
!! GGGTCTTTAGAGTAACCCAA+AGG + chr6_3:30464561-30464580 None:intergenic 45.0%
GGTTAACACAGTGCAATGAG+TGG - chr6_3:30464745-30464764 Msa0947550:intron 45.0%
CAAACAGCATATCCAGGAAC+TGG - chr6_3:30465192-30465211 Msa0947550:intron 45.0%
ATATCCAGGAACTGGACCTT+TGG - chr6_3:30465200-30465219 Msa0947550:intron 45.0%
GGAGAATTTGGCAACACACT+TGG - chr6_3:30465221-30465240 Msa0947550:CDS 45.0%
GATCCATGGCACAACAACAT+TGG - chr6_3:30465246-30465265 Msa0947550:CDS 45.0%
GAGGGAGATATCTATTGAGG+TGG + chr6_3:30465285-30465304 None:intergenic 45.0%
!!! ATAATATATATTTTTACTAA+TGG - chr6_3:30463307-30463326 Msa0947550:intron 5.0%
CCATCAAGGTAAGGTGGTCT+AGG + chr6_3:30463096-30463115 None:intergenic 50.0%
CCTAGACCACCTTACCTTGA+TGG - chr6_3:30463093-30463112 Msa0947550:CDS 50.0%
GCTGAACCATCAAGGTAAGG+TGG + chr6_3:30463102-30463121 None:intergenic 50.0%
GGAGCTGAACCATCAAGGTA+AGG + chr6_3:30463105-30463124 None:intergenic 50.0%
! TACCTTGATGGTTCAGCTCC+AGG - chr6_3:30463105-30463124 Msa0947550:CDS 50.0%
! ACCTTGATGGTTCAGCTCCA+GGG - chr6_3:30463106-30463125 Msa0947550:CDS 50.0%
CTTGATCCAACGGTCACTGA+TGG - chr6_3:30463559-30463578 Msa0947550:intron 50.0%
ATCGGCCCATCAATGTCTGT+TGG - chr6_3:30464087-30464106 Msa0947550:intron 50.0%
! ATGCATGCAGCCAGGGATTT+TGG - chr6_3:30464323-30464342 Msa0947550:intron 50.0%
!! GTACCAGAGGCATTGGGATT+AGG - chr6_3:30464345-30464364 Msa0947550:intron 50.0%
TTGCCTAATCCCAATGCCTC+TGG + chr6_3:30464351-30464370 None:intergenic 50.0%
!! AGGCATTGGGATTAGGCAAC+TGG - chr6_3:30464352-30464371 Msa0947550:intron 50.0%
! GGCATTGGGATTAGGCAACT+GGG - chr6_3:30464353-30464372 Msa0947550:intron 50.0%
! ACTGGGTGAAAGCACAGGAA+TGG - chr6_3:30464370-30464389 Msa0947550:intron 50.0%
! CTGGGTGAAAGCACAGGAAT+GGG - chr6_3:30464371-30464390 Msa0947550:intron 50.0%
AGTACCACCTTTCACACACG+CGG - chr6_3:30464724-30464743 Msa0947550:intron 50.0%
TTAACCGCGTGTGTGAAAGG+TGG + chr6_3:30464731-30464750 None:intergenic 50.0%
GTGTTAACCGCGTGTGTGAA+AGG + chr6_3:30464734-30464753 None:intergenic 50.0%
TTCTCCAAAGGTCCAGTTCC+TGG + chr6_3:30465207-30465226 None:intergenic 50.0%
CAACACACTTGGCTGATCCA+TGG - chr6_3:30465232-30465251 Msa0947550:CDS 50.0%
TAAGGTGGTCTAGGCTGACC+AGG + chr6_3:30463087-30463106 None:intergenic 55.0%
ACCCTGGAGCTGAACCATCA+AGG + chr6_3:30463110-30463129 None:intergenic 55.0%
ATGGGCCGACTGCATGAATG+CGG - chr6_3:30463578-30463597 Msa0947550:intron 55.0%
GGTACTTCACCCAGACGTAG+AGG + chr6_3:30463784-30463803 None:intergenic 55.0%
!! GCCAGGGATTTTGGTACCAG+AGG - chr6_3:30464332-30464351 Msa0947550:intron 55.0%
GCCTCTGGTACCAAAATCCC+TGG + chr6_3:30464336-30464355 None:intergenic 55.0%
! AGGCAACTGGGTGAAAGCAC+AGG - chr6_3:30464365-30464384 Msa0947550:intron 55.0%
AGGTAGGTTGCTTGTCCTCC+AGG + chr6_3:30464405-30464424 None:intergenic 55.0%
GGAGGACAAGCAACCTACCT+AGG - chr6_3:30464405-30464424 Msa0947550:intron 55.0%
TGGAACAGGGTTGCCTAGGT+AGG + chr6_3:30464421-30464440 None:intergenic 55.0%
ACCTAGGCAACCCTGTTCCA+TGG - chr6_3:30464421-30464440 Msa0947550:intron 55.0%
CCTAGGCAACCCTGTTCCAT+GGG - chr6_3:30464422-30464441 Msa0947550:intron 55.0%
CTAGGCAACCCTGTTCCATG+GGG - chr6_3:30464423-30464442 Msa0947550:intron 55.0%
CAGGAATGGGCAGCACTTCC+TGG - chr6_3:30464384-30464403 Msa0947550:intron 60.0%
GAATGGGCAGCACTTCCTGG+AGG - chr6_3:30464387-30464406 Msa0947550:intron 60.0%
CCCATGGAACAGGGTTGCCT+AGG + chr6_3:30464425-30464444 None:intergenic 60.0%
! GTAGGGTGCCCCATGGAACA+GGG + chr6_3:30464434-30464453 None:intergenic 60.0%
! GGGCACCCTACCCACCATTT+TGG - chr6_3:30464443-30464462 Msa0947550:intron 60.0%
GTCGGCCCATCAGTGACCGT+TGG + chr6_3:30463568-30463587 None:intergenic 65.0%
! GGTAGGGTGCCCCATGGAAC+AGG + chr6_3:30464435-30464454 None:intergenic 65.0%
!! ATGGTGGGTAGGGTGCCCCA+TGG + chr6_3:30464441-30464460 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr6_3 gene 30462799 30465547 30462799 ID=Msa0947550;Name=Msa0947550
chr6_3 mRNA 30462799 30465547 30462799 ID=Msa0947550-mRNA-1;Parent=Msa0947550;Name=Msa0947550-mRNA-1;_AED=0.01;_eAED=0.01;_QI=144|1|1|1|1|1|4|254|239
chr6_3 exon 30462799 30463195 30462799 ID=Msa0947550-mRNA-1:exon:8041;Parent=Msa0947550-mRNA-1
chr6_3 exon 30463725 30464013 30463725 ID=Msa0947550-mRNA-1:exon:8040;Parent=Msa0947550-mRNA-1
chr6_3 exon 30464479 30464581 30464479 ID=Msa0947550-mRNA-1:exon:8039;Parent=Msa0947550-mRNA-1
chr6_3 exon 30465219 30465547 30465219 ID=Msa0947550-mRNA-1:exon:8038;Parent=Msa0947550-mRNA-1
chr6_3 five_prime_UTR 30465404 30465547 30465404 ID=Msa0947550-mRNA-1:five_prime_utr;Parent=Msa0947550-mRNA-1
chr6_3 CDS 30465219 30465403 30465219 ID=Msa0947550-mRNA-1:cds;Parent=Msa0947550-mRNA-1
chr6_3 CDS 30464479 30464581 30464479 ID=Msa0947550-mRNA-1:cds;Parent=Msa0947550-mRNA-1
chr6_3 CDS 30463725 30464013 30463725 ID=Msa0947550-mRNA-1:cds;Parent=Msa0947550-mRNA-1
chr6_3 CDS 30463053 30463195 30463053 ID=Msa0947550-mRNA-1:cds;Parent=Msa0947550-mRNA-1
chr6_3 three_prime_UTR 30462799 30463052 30462799 ID=Msa0947550-mRNA-1:three_prime_utr;Parent=Msa0947550-mRNA-1
Gene Sequence

>Msa0947550

ATGGCTTGTAACACATTGATGAGTTCTGCTATCTCAGCATTTCCATCACTTCTTTCTTCCTCAAAATCAAGATTTTCAACTTCACTTCCACTTCCTTGCACAAATGCTTCTTCTAGATTCACCATGACTTCTGATTGGATGCCTGGTCAGCCTAGACCACCTTACCTTGATGGTTCAGCTCCAGGTGACTTTGGATTTGATCCTCTACGTCTGGGTGAAGTACCTGAGAATCTTGAGAGATTCAAAGAGTCTGAACTCATTCACTGCAGATGGGCCATGCTTGCTGTTCCAGGGATTTTGGTACCAGAGGCATTGGGATTAGGCAACTGGGTGAAAGCACAGGAATGGGCAGCACTTCCTGGAGGACAAGCAACCTACCTAGGCAACCCTGTTCCATGGGGCACCCTACCCACCATTTTGGTCATTGAATTCCTTTCTATTGCTTTTGTTGAGCACCAAAGGAGTATGGAGAAAGACCCTGAAAAGAAGAAGTACCCTGGTGGTGCTTTTGATCCTTTGGGTTACTCTAAAGACCCTAAAAAGTTTCATGAATACAAAATCAAAGAAGTTAAGAATGGACGTCTTGCTTTGTTGGCTTTTGTTGGAATCTGTGTTCAACAAACAGCATATCCAGGAACTGGACCTTTGGAGAATTTGGCAACACACTTGGCTGATCCATGGCACAACAACATTGGAAATGTCCTTATTCCACCTCAATAG
Protein sequence

>Msa0947550

MACNTLMSSAISAFPSLLSSSKSRFSTSLPLPCTNASSRFTMTSDWMPGQPRPPYLDGSAPGDFGFDPLRLGEVPENLERFKESELIHCRWAMLAVPGILVPEALGLGNWVKAQEWAALPGGQATYLGNPVPWGTLPTILVIEFLSIAFVEHQRSMEKDPEKKKYPGGAFDPLGYSKDPKKFHEYKIKEVKNGRLALLAFVGICVQQTAYPGTGPLENLATHLADPWHNNIGNVLIPPQ*