AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0921340


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0921340 MtrunA17_Chr6g0478031 97.452 157 4 0 1 157 1 157 4.15e-110 309
Msa0921340 MtrunA17_Chr2g0303931 75.524 143 35 0 1 143 6 148 7.15e-71 210
Msa0921340 MtrunA17_Chr4g0065951 46.617 133 71 0 16 148 21 153 3.86e-35 120
Msa0921340 MtrunA17_Chr1g0199041 42.568 148 71 4 2 148 6 140 1.08e-26 99.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0921340 AT5G46530.1 72.000 150 42 0 1 150 6 155 4.07e-77 226
Msa0921340 AT1G29520.1 78.667 150 32 0 1 150 6 155 5.29e-76 224
Msa0921340 AT5G18970.1 45.578 147 80 0 2 148 7 153 1.33e-37 127
Msa0921340 AT1G04560.1 45.270 148 67 4 2 148 7 141 1.29e-30 109

Find 29 sgRNAs with CRISPR-Local

Find 123 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTTCCCATTGGGAAGAAAA+TGG 0.238328 6_2:-58596289 None:intergenic
TGCATGTATGTCATTGTTCT+AGG 0.248721 6_2:+58596016 Msa0921340:CDS
CTCTACATAGTTGCAATTCA+TGG 0.273645 6_2:+58597125 Msa0921340:CDS
GGTGCTGCATCGACAATTTC+AGG 0.318250 6_2:+58596360 Msa0921340:CDS
TGCTGCTTCAATTGCTACTT+TGG 0.334645 6_2:+58596425 Msa0921340:CDS
CCAATGGGAAATGCTTCAAC+TGG 0.374176 6_2:+58596300 Msa0921340:CDS
CTTCAATTGCTACTTTGGCT+TGG 0.384442 6_2:+58596430 Msa0921340:CDS
GACTCTTACACTGCTTGCCA+TGG 0.388463 6_2:+58596452 Msa0921340:CDS
GTTACGATTTCTTTGTTAGC+TGG 0.398205 6_2:+58596330 Msa0921340:CDS
TCTTTGTTAGCTGGAGTAGT+TGG 0.415192 6_2:+58596339 Msa0921340:CDS
TTGTTCTAGGCATTGGTGCA+TGG 0.424644 6_2:+58596029 Msa0921340:CDS
CAGTTGAAGCATTTCCCATT+GGG 0.433142 6_2:-58596299 None:intergenic
TGAGCTGGAAGATTTAATTC+CGG 0.439380 6_2:-58596258 None:intergenic
TGTTCTAGGCATTGGTGCAT+GGG 0.449144 6_2:+58596030 Msa0921340:CDS
ACTCTTACACTGCTTGCCAT+GGG 0.453490 6_2:+58596453 Msa0921340:CDS
ACAAAATACATAGTTCGGTT+AGG 0.457892 6_2:+58595949 None:intergenic
TTTCCCATTGGGAAGAAAAT+GGG 0.461124 6_2:-58596288 None:intergenic
GAGTCAAACATATCTGTTCA+TGG 0.465531 6_2:+58596382 Msa0921340:CDS
TCAAATTAGAAATGCACGTC+TGG 0.465623 6_2:+58596979 Msa0921340:CDS
CCAGTTGAAGCATTTCCCAT+TGG 0.492597 6_2:-58596300 None:intergenic
GATAATTATGAAAGCCTCCA+TGG 0.495765 6_2:-58597084 None:intergenic
TATGTCATTGTTCTAGGCAT+TGG 0.506725 6_2:+58596022 Msa0921340:CDS
GTCCATAGCAACACTTCTCT+TGG 0.510981 6_2:+58595982 Msa0921340:CDS
CACCAAGAGAAGTGTTGCTA+TGG 0.525202 6_2:-58595984 None:intergenic
TTAACTTCTGCAGATTTGCA+TGG 0.530112 6_2:+58596942 Msa0921340:intron
ATGAATAGAGCAATAGATCA+TGG 0.550315 6_2:+58596055 Msa0921340:CDS
AGAGAAGCTGTGTAACTGAA+AGG 0.596446 6_2:-58597106 None:intergenic
GTAGCAATTGAAGCAGCAGA+TGG 0.611432 6_2:-58596420 None:intergenic
CTCTTACACTGCTTGCCATG+GGG 0.615745 6_2:+58596454 Msa0921340:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTATTACTATTATTTAG+AGG - chr6_2:58596520-58596539 None:intergenic 10.0%
!! AATAAAACTATAAAAATCAA+AGG + chr6_2:58596653-58596672 Msa0921340:intron 10.0%
!! CATATAATATATATATTTGA+CGG + chr6_2:58597156-58597175 Msa0921340:CDS 10.0%
!!! TTTTTATTACTATTATTTAG+AGG - chr6_2:58596520-58596539 None:intergenic 10.0%
!! AATAAAACTATAAAAATCAA+AGG + chr6_2:58596653-58596672 Msa0921340:intron 10.0%
!!! GTTTTAATTGAAAACAAATT+TGG - chr6_2:58596875-58596894 None:intergenic 15.0%
!!! GAATCATTTAATAATTTTCT+TGG + chr6_2:58597015-58597034 Msa0921340:intron 15.0%
!!! GTTTTAATTGAAAACAAATT+TGG - chr6_2:58596875-58596894 None:intergenic 15.0%
!!! GAATCATTTAATAATTTTCT+TGG + chr6_2:58597015-58597034 Msa0921340:intron 15.0%
!!! TTCTGACATTCTAAAATATT+TGG - chr6_2:58596123-58596142 None:intergenic 20.0%
!! ATTACTATTATTTAGAGGTA+TGG - chr6_2:58596515-58596534 None:intergenic 20.0%
!!! TTCTGACATTCTAAAATATT+TGG - chr6_2:58596123-58596142 None:intergenic 20.0%
!! ATTACTATTATTTAGAGGTA+TGG - chr6_2:58596515-58596534 None:intergenic 20.0%
!!! ATAGATCATGGTTTTATCAT+AGG + chr6_2:58596067-58596086 Msa0921340:CDS 25.0%
! TTAGAATGTCAGAAAAACAA+TGG + chr6_2:58596129-58596148 Msa0921340:intron 25.0%
!!! ATTTTGTTATGAAATTACGC+AGG + chr6_2:58596234-58596253 Msa0921340:intron 25.0%
!! TTGATTTTATCCATTCCTAT+AGG + chr6_2:58596584-58596603 Msa0921340:intron 25.0%
! CAATGAATTTCGTAAAATCA+TGG + chr6_2:58596788-58596807 Msa0921340:intron 25.0%
!! TGTAACTTTTGTCAAAATCA+CGG + chr6_2:58596841-58596860 Msa0921340:intron 25.0%
!!! ATAGATCATGGTTTTATCAT+AGG + chr6_2:58596067-58596086 Msa0921340:CDS 25.0%
! TTAGAATGTCAGAAAAACAA+TGG + chr6_2:58596129-58596148 Msa0921340:intron 25.0%
!!! ATTTTGTTATGAAATTACGC+AGG + chr6_2:58596234-58596253 Msa0921340:intron 25.0%
!! TTGATTTTATCCATTCCTAT+AGG + chr6_2:58596584-58596603 Msa0921340:intron 25.0%
! CAATGAATTTCGTAAAATCA+TGG + chr6_2:58596788-58596807 Msa0921340:intron 25.0%
!! TGTAACTTTTGTCAAAATCA+CGG + chr6_2:58596841-58596860 Msa0921340:intron 25.0%
ATGAATAGAGCAATAGATCA+TGG + chr6_2:58596055-58596074 Msa0921340:CDS 30.0%
ATTTGGCAAATGTGAAAGAT+AGG - chr6_2:58596106-58596125 None:intergenic 30.0%
TAAGTCTATCAATCACCTAT+AGG - chr6_2:58596602-58596621 None:intergenic 30.0%
! AAATCAAAGGAGCGTATTAA+AGG + chr6_2:58596666-58596685 Msa0921340:intron 30.0%
! AATCAAAGGAGCGTATTAAA+GGG + chr6_2:58596667-58596686 Msa0921340:intron 30.0%
ATGAATAGAGCAATAGATCA+TGG + chr6_2:58596055-58596074 Msa0921340:CDS 30.0%
ATTTGGCAAATGTGAAAGAT+AGG - chr6_2:58596106-58596125 None:intergenic 30.0%
TAAGTCTATCAATCACCTAT+AGG - chr6_2:58596602-58596621 None:intergenic 30.0%
! AAATCAAAGGAGCGTATTAA+AGG + chr6_2:58596666-58596685 Msa0921340:intron 30.0%
! AATCAAAGGAGCGTATTAAA+GGG + chr6_2:58596667-58596686 Msa0921340:intron 30.0%
TGCATGTATGTCATTGTTCT+AGG + chr6_2:58596016-58596035 Msa0921340:CDS 35.0%
! TATGTCATTGTTCTAGGCAT+TGG + chr6_2:58596022-58596041 Msa0921340:CDS 35.0%
TTACCGGACTTGAATTACTT+CGG + chr6_2:58596204-58596223 Msa0921340:intron 35.0%
TGAGCTGGAAGATTTAATTC+CGG - chr6_2:58596261-58596280 None:intergenic 35.0%
AAAATGGGTGAAAAATGAGC+TGG - chr6_2:58596276-58596295 None:intergenic 35.0%
TTTCCCATTGGGAAGAAAAT+GGG - chr6_2:58596291-58596310 None:intergenic 35.0%
ATTTCCCATTGGGAAGAAAA+TGG - chr6_2:58596292-58596311 None:intergenic 35.0%
GTTACGATTTCTTTGTTAGC+TGG + chr6_2:58596330-58596349 Msa0921340:CDS 35.0%
GAGTCAAACATATCTGTTCA+TGG + chr6_2:58596382-58596401 Msa0921340:CDS 35.0%
AAAGAGAGAAATTTACCCCA+TGG - chr6_2:58596472-58596491 None:intergenic 35.0%
CTATCAATCACCTATAGGAA+TGG - chr6_2:58596597-58596616 None:intergenic 35.0%
! TGCTCTTCTTTTATCTTACC+AGG + chr6_2:58596749-58596768 Msa0921340:intron 35.0%
TTAACTTCTGCAGATTTGCA+TGG + chr6_2:58596942-58596961 Msa0921340:intron 35.0%
TCAAATTAGAAATGCACGTC+TGG + chr6_2:58596979-58596998 Msa0921340:CDS 35.0%
GATAATTATGAAAGCCTCCA+TGG - chr6_2:58597087-58597106 None:intergenic 35.0%
CTCTACATAGTTGCAATTCA+TGG + chr6_2:58597125-58597144 Msa0921340:CDS 35.0%
TGCATGTATGTCATTGTTCT+AGG + chr6_2:58596016-58596035 Msa0921340:CDS 35.0%
! TATGTCATTGTTCTAGGCAT+TGG + chr6_2:58596022-58596041 Msa0921340:CDS 35.0%
TTACCGGACTTGAATTACTT+CGG + chr6_2:58596204-58596223 Msa0921340:intron 35.0%
TGAGCTGGAAGATTTAATTC+CGG - chr6_2:58596261-58596280 None:intergenic 35.0%
AAAATGGGTGAAAAATGAGC+TGG - chr6_2:58596276-58596295 None:intergenic 35.0%
TTTCCCATTGGGAAGAAAAT+GGG - chr6_2:58596291-58596310 None:intergenic 35.0%
ATTTCCCATTGGGAAGAAAA+TGG - chr6_2:58596292-58596311 None:intergenic 35.0%
GTTACGATTTCTTTGTTAGC+TGG + chr6_2:58596330-58596349 Msa0921340:CDS 35.0%
GAGTCAAACATATCTGTTCA+TGG + chr6_2:58596382-58596401 Msa0921340:CDS 35.0%
AAAGAGAGAAATTTACCCCA+TGG - chr6_2:58596472-58596491 None:intergenic 35.0%
CTATCAATCACCTATAGGAA+TGG - chr6_2:58596597-58596616 None:intergenic 35.0%
! TGCTCTTCTTTTATCTTACC+AGG + chr6_2:58596749-58596768 Msa0921340:intron 35.0%
TTAACTTCTGCAGATTTGCA+TGG + chr6_2:58596942-58596961 Msa0921340:intron 35.0%
TCAAATTAGAAATGCACGTC+TGG + chr6_2:58596979-58596998 Msa0921340:CDS 35.0%
GATAATTATGAAAGCCTCCA+TGG - chr6_2:58597087-58597106 None:intergenic 35.0%
CTCTACATAGTTGCAATTCA+TGG + chr6_2:58597125-58597144 Msa0921340:CDS 35.0%
! ATTTTTCGCAGCTATTTGCG+TGG + chr6_2:58596164-58596183 Msa0921340:intron 40.0%
AACGTGTGAGTTAGGTTTAC+CGG + chr6_2:58596188-58596207 Msa0921340:intron 40.0%
ATGCCGAAGTAATTCAAGTC+CGG - chr6_2:58596210-58596229 None:intergenic 40.0%
GTTATGAAATTACGCAGGTC+CGG + chr6_2:58596239-58596258 Msa0921340:intron 40.0%
! TTCACCCATTTTCTTCCCAA+TGG + chr6_2:58596284-58596303 Msa0921340:CDS 40.0%
! TCACCCATTTTCTTCCCAAT+GGG + chr6_2:58596285-58596304 Msa0921340:CDS 40.0%
! CAGTTGAAGCATTTCCCATT+GGG - chr6_2:58596302-58596321 None:intergenic 40.0%
TCTTTGTTAGCTGGAGTAGT+TGG + chr6_2:58596339-58596358 Msa0921340:CDS 40.0%
! TGCTGCTTCAATTGCTACTT+TGG + chr6_2:58596425-58596444 Msa0921340:CDS 40.0%
!! CTTCAATTGCTACTTTGGCT+TGG + chr6_2:58596430-58596449 Msa0921340:CDS 40.0%
TATCTTACCAGGTGCATGTT+TGG + chr6_2:58596760-58596779 Msa0921340:intron 40.0%
! CCATGGTTTTCTGCAAAAAG+AGG - chr6_2:58597070-58597089 None:intergenic 40.0%
! CCTCTTTTTGCAGAAAACCA+TGG + chr6_2:58597067-58597086 Msa0921340:intron 40.0%
! CTTTTTGCAGAAAACCATGG+AGG + chr6_2:58597070-58597089 Msa0921340:intron 40.0%
AGAGAAGCTGTGTAACTGAA+AGG - chr6_2:58597109-58597128 None:intergenic 40.0%
! ATTTTTCGCAGCTATTTGCG+TGG + chr6_2:58596164-58596183 Msa0921340:intron 40.0%
AACGTGTGAGTTAGGTTTAC+CGG + chr6_2:58596188-58596207 Msa0921340:intron 40.0%
ATGCCGAAGTAATTCAAGTC+CGG - chr6_2:58596210-58596229 None:intergenic 40.0%
GTTATGAAATTACGCAGGTC+CGG + chr6_2:58596239-58596258 Msa0921340:intron 40.0%
! TTCACCCATTTTCTTCCCAA+TGG + chr6_2:58596284-58596303 Msa0921340:CDS 40.0%
! TCACCCATTTTCTTCCCAAT+GGG + chr6_2:58596285-58596304 Msa0921340:CDS 40.0%
! CAGTTGAAGCATTTCCCATT+GGG - chr6_2:58596302-58596321 None:intergenic 40.0%
TCTTTGTTAGCTGGAGTAGT+TGG + chr6_2:58596339-58596358 Msa0921340:CDS 40.0%
! TGCTGCTTCAATTGCTACTT+TGG + chr6_2:58596425-58596444 Msa0921340:CDS 40.0%
!! CTTCAATTGCTACTTTGGCT+TGG + chr6_2:58596430-58596449 Msa0921340:CDS 40.0%
TATCTTACCAGGTGCATGTT+TGG + chr6_2:58596760-58596779 Msa0921340:intron 40.0%
! CCATGGTTTTCTGCAAAAAG+AGG - chr6_2:58597070-58597089 None:intergenic 40.0%
! CCTCTTTTTGCAGAAAACCA+TGG + chr6_2:58597067-58597086 Msa0921340:intron 40.0%
! CTTTTTGCAGAAAACCATGG+AGG + chr6_2:58597070-58597089 Msa0921340:intron 40.0%
AGAGAAGCTGTGTAACTGAA+AGG - chr6_2:58597109-58597128 None:intergenic 40.0%
GTCCATAGCAACACTTCTCT+TGG + chr6_2:58595982-58596001 Msa0921340:CDS 45.0%
!! CACCAAGAGAAGTGTTGCTA+TGG - chr6_2:58595987-58596006 None:intergenic 45.0%
!! TTGTTCTAGGCATTGGTGCA+TGG + chr6_2:58596029-58596048 Msa0921340:CDS 45.0%
!! TGTTCTAGGCATTGGTGCAT+GGG + chr6_2:58596030-58596049 Msa0921340:CDS 45.0%
! CCAGTTGAAGCATTTCCCAT+TGG - chr6_2:58596303-58596322 None:intergenic 45.0%
CCAATGGGAAATGCTTCAAC+TGG + chr6_2:58596300-58596319 Msa0921340:CDS 45.0%
GTAGCAATTGAAGCAGCAGA+TGG - chr6_2:58596423-58596442 None:intergenic 45.0%
ACTCTTACACTGCTTGCCAT+GGG + chr6_2:58596453-58596472 Msa0921340:CDS 45.0%
TGTCAATCCAAACATGCACC+TGG - chr6_2:58596770-58596789 None:intergenic 45.0%
GTCCATAGCAACACTTCTCT+TGG + chr6_2:58595982-58596001 Msa0921340:CDS 45.0%
!! CACCAAGAGAAGTGTTGCTA+TGG - chr6_2:58595987-58596006 None:intergenic 45.0%
!! TTGTTCTAGGCATTGGTGCA+TGG + chr6_2:58596029-58596048 Msa0921340:CDS 45.0%
!! TGTTCTAGGCATTGGTGCAT+GGG + chr6_2:58596030-58596049 Msa0921340:CDS 45.0%
! CCAGTTGAAGCATTTCCCAT+TGG - chr6_2:58596303-58596322 None:intergenic 45.0%
CCAATGGGAAATGCTTCAAC+TGG + chr6_2:58596300-58596319 Msa0921340:CDS 45.0%
GTAGCAATTGAAGCAGCAGA+TGG - chr6_2:58596423-58596442 None:intergenic 45.0%
ACTCTTACACTGCTTGCCAT+GGG + chr6_2:58596453-58596472 Msa0921340:CDS 45.0%
TGTCAATCCAAACATGCACC+TGG - chr6_2:58596770-58596789 None:intergenic 45.0%
! TGCGTGGTAACGTGTGAGTT+AGG + chr6_2:58596180-58596199 Msa0921340:intron 50.0%
! GGTGCTGCATCGACAATTTC+AGG + chr6_2:58596360-58596379 Msa0921340:CDS 50.0%
GACTCTTACACTGCTTGCCA+TGG + chr6_2:58596452-58596471 Msa0921340:CDS 50.0%
CTCTTACACTGCTTGCCATG+GGG + chr6_2:58596454-58596473 Msa0921340:CDS 50.0%
! TGCGTGGTAACGTGTGAGTT+AGG + chr6_2:58596180-58596199 Msa0921340:intron 50.0%
! GGTGCTGCATCGACAATTTC+AGG + chr6_2:58596360-58596379 Msa0921340:CDS 50.0%
GACTCTTACACTGCTTGCCA+TGG + chr6_2:58596452-58596471 Msa0921340:CDS 50.0%
CTCTTACACTGCTTGCCATG+GGG + chr6_2:58596454-58596473 Msa0921340:CDS 50.0%
Chromosome Type Strat End Strand Name
chr6_2 gene 58595964 58597175 58595964 ID=Msa0921340;Name=Msa0921340
chr6_2 mRNA 58595964 58597175 58595964 ID=Msa0921340-mRNA-1;Parent=Msa0921340;Name=Msa0921340-mRNA-1;_AED=0.05;_eAED=0.05;_QI=13|1|0.75|1|1|1|4|0|157
chr6_2 exon 58595964 58596088 58595964 ID=Msa0921340-mRNA-1:exon:8720;Parent=Msa0921340-mRNA-1
chr6_2 exon 58596256 58596475 58596256 ID=Msa0921340-mRNA-1:exon:8721;Parent=Msa0921340-mRNA-1
chr6_2 exon 58596955 58597000 58596955 ID=Msa0921340-mRNA-1:exon:8722;Parent=Msa0921340-mRNA-1
chr6_2 exon 58597080 58597175 58597080 ID=Msa0921340-mRNA-1:exon:8723;Parent=Msa0921340-mRNA-1
chr6_2 five_prime_UTR 58595964 58595976 58595964 ID=Msa0921340-mRNA-1:five_prime_utr;Parent=Msa0921340-mRNA-1
chr6_2 CDS 58595977 58596088 58595977 ID=Msa0921340-mRNA-1:cds;Parent=Msa0921340-mRNA-1
chr6_2 CDS 58596256 58596475 58596256 ID=Msa0921340-mRNA-1:cds;Parent=Msa0921340-mRNA-1
chr6_2 CDS 58596955 58597000 58596955 ID=Msa0921340-mRNA-1:cds;Parent=Msa0921340-mRNA-1
chr6_2 CDS 58597080 58597175 58597080 ID=Msa0921340-mRNA-1:cds;Parent=Msa0921340-mRNA-1
Gene Sequence

>Msa0921340

ATGAAGTCCATAGCAACACTTCTCTTGGTGCTCAACTTTTGCATGTATGTCATTGTTCTAGGCATTGGTGCATGGGCTATGAATAGAGCAATAGATCATGGTTTTATCATAGGTCCGGAATTAAATCTTCCAGCTCATTTTTCACCCATTTTCTTCCCAATGGGAAATGCTTCAACTGGATTCTTTGTTACGATTTCTTTGTTAGCTGGAGTAGTTGGTGCTGCATCGACAATTTCAGGAGTCAAACATATCTGTTCATGGACTGCAGAGAGCTTGCCATCTGCTGCTTCAATTGCTACTTTGGCTTGGACTCTTACACTGCTTGCCATGGGATTTGCATGGAAAGAGATTGAGCTTCAAATTAGAAATGCACGTCTGAAAACCATGGAGGCTTTCATAATTATCCTTTCAGTTACACAGCTTCTCTACATAGTTGCAATTCATGGTGTTGCTGCATATAATATATATATTTGA

Protein sequence

>Msa0921340

MKSIATLLLVLNFCMYVIVLGIGAWAMNRAIDHGFIIGPELNLPAHFSPIFFPMGNASTGFFVTISLLAGVVGAASTISGVKHICSWTAESLPSAASIATLAWTLTLLAMGFAWKEIELQIRNARLKTMEAFIIILSVTQLLYIVAIHGVAAYNIYI*