AlfalfaGEDB | Msa1186150

M. sativa cultivar zhongmu-4 / Msa1186150

KEGG: K22324
GO: GO:0003674 GO:0003824 GO:0004497 GO:0008150 GO:0008152 GO:0016491 GO:0055114
PFAM: PF00743.22

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1186150	XP_003627754.1	94.444	432	24	0	1	432	1	432	0.0	851

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1186150	sp\|Q9SXD5\|GSXL3_ARATH	54.439	428	182	5	4	421	13	437	1.56e-163	471

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0004180	Msa1186150	-0.830269	3.229329e-55	-8.615850e-47
Msa0005560	Msa1186150	0.812488	4.135053e-51	-8.615850e-47
Msa0027020	Msa1186150	-0.814409	1.563173e-51	-8.615850e-47
Msa0042210	Msa1186150	-0.815909	7.258727e-52	-8.615850e-47
Msa0047560	Msa1186150	-0.802694	4.967453e-49	-8.615850e-47
Msa0070120	Msa1186150	0.815018	1.146140e-51	-8.615850e-47
Msa0075080	Msa1186150	-0.820875	5.442424e-53	-8.615850e-47
Msa0075820	Msa1186150	-0.805911	1.062189e-49	-8.615850e-47
Msa0080100	Msa1186150	-0.825271	5.134453e-54	-8.615850e-47
Msa0093360	Msa1186150	-0.824153	9.417827e-54	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1186150	MtrunA17_Chr8g0352461	94.444	432	24	0	1	432	1	432	0.0	851
Msa1186150	MtrunA17_Chr8g0352481	87.269	432	54	1	1	432	1	431	0.0	787
Msa1186150	MtrunA17_Chr5g0399001	54.481	424	186	3	4	420	33	456	5.29e-160	459
Msa1186150	MtrunA17_Chr3g0091441	42.923	431	226	7	4	420	13	437	8.11e-117	349
Msa1186150	MtrunA17_Chr7g0268621	24.460	278	176	5	4	279	6	251	1.12e-18	87.4
Msa1186150	MtrunA17_Chr1g0148801	25.571	219	134	6	6	223	18	208	8.30e-18	85.1
Msa1186150	MtrunA17_Chr3g0139441	27.143	210	124	6	6	212	41	224	3.32e-17	83.2
Msa1186150	MtrunA17_Chr2g0304651	26.482	253	129	8	3	222	4	232	8.89e-16	79.3
Msa1186150	MtrunA17_Chr2g0305211	26.337	243	122	8	4	213	5	223	2.74e-15	77.8
Msa1186150	MtrunA17_Chr2g0305231	26.337	243	122	8	4	213	5	223	2.74e-15	77.8

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1186150	AT1G62620.1	54.439	428	182	5	4	421	13	437	1.59e-164	471
Msa1186150	AT1G63370.1	53.972	428	184	5	4	421	13	437	1.09e-162	466
Msa1186150	AT1G12140.1	52.632	437	195	4	2	427	11	446	2.86e-157	452
Msa1186150	AT1G12140.3	52.632	437	195	4	2	427	11	446	2.86e-157	452
Msa1186150	AT1G12200.1	53.037	428	190	4	4	421	14	440	1.50e-155	448
Msa1186150	AT1G12140.2	52.174	437	195	5	2	427	11	444	5.93e-154	444
Msa1186150	AT1G62600.1	52.459	427	194	3	4	421	13	439	6.72e-154	444
Msa1186150	AT1G62620.2	56.800	375	155	3	4	371	13	387	3.36e-151	434
Msa1186150	AT1G12160.1	49.203	439	198	6	4	420	12	447	2.04e-149	433
Msa1186150	AT1G65860.1	50.000	422	205	4	4	420	13	433	4.29e-149	432

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Find 126 sgRNAs with CRISPR-Local

Find 328 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACCAATGAAAGAGAGCCA+TGG	0.673851	8_1:-24188142	None:intergenic
AAAGTTGCAGTGATCGGCGC+CGG	0.565896	8_1:+24183949	Msa1186150:CDS
AAATCTTCCAAGACATATCA+TGG	0.419741	8_1:+24184152	Msa1186150:CDS
AACTTCCTACCCTTCAAATC+TGG	0.337087	8_1:-24187646	None:intergenic
AAGAACCCCATGATATGTCT+TGG	0.495350	8_1:-24184159	None:intergenic
AAGACCGAAACCAGATTTGA+AGG	0.481017	8_1:+24187636	Msa1186150:CDS
AAGCGAATGAGTTTGACGCT+TGG	0.580224	8_1:-24188417	None:intergenic
AATCTTCCAAGACATATCAT+GGG	0.579372	8_1:+24184153	Msa1186150:CDS
AATGTTGATTGTTAAATTGG+AGG	0.520725	8_1:+24188970	Msa1186150:three_prime_UTR
ACAACCGCATCGGAGGAACT+TGG	0.526800	8_1:+24184040	Msa1186150:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CTAAAACAATCTTGTCTTGA+TGG	+	chr8_1:24183924-24183943	Msa1186150:exon	30.0%
AAATCTTCCAAGACATATCA+TGG	+	chr8_1:24184152-24184171	Msa1186150:CDS	30.0%
AATCTTCCAAGACATATCAT+GGG	+	chr8_1:24184153-24184172	Msa1186150:CDS	30.0%
CATTCAATAAATGACGTATG+TGG	+	chr8_1:24184576-24184595	Msa1186150:intron	30.0%
ATGACATGTCTTGTCTTTAT+TGG	-	chr8_1:24184981-24185000	None:intergenic	30.0%
AAGAAGAATTGGTAAAGTAG+TGG	+	chr8_1:24185699-24185718	Msa1186150:intron	30.0%
AGAAGAATTGGTAAAGTAGT+GGG	+	chr8_1:24185700-24185719	Msa1186150:intron	30.0%
TGTCTCTTCGAAATTAAGAA+TGG	-	chr8_1:24186419-24186438	None:intergenic	30.0%
CGTAAGAACACTCTTAATTA+AGG	+	chr8_1:24186483-24186502	Msa1186150:intron	30.0%
ACTTGACCTGAAATTTACTT+TGG	-	chr8_1:24186809-24186828	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_1	gene	24183814	24189050	24183814	ID=Msa1186150;Name=Msa1186150
chr8_1	mRNA	24183814	24189050	24183814	ID=Msa1186150-mRNA-1;Parent=Msa1186150;Name=Msa1186150-mRNA-1;_AED=0.11;_eAED=0.11;_QI=129\|1\|1\|1\|1\|1\|6\|110\|432
chr8_1	exon	24183814	24184521	24183814	ID=Msa1186150-mRNA-1:exon:8799;Parent=Msa1186150-mRNA-1
chr8_1	exon	24187550	24187702	24187550	ID=Msa1186150-mRNA-1:exon:8800;Parent=Msa1186150-mRNA-1
chr8_1	exon	24187799	24187881	24187799	ID=Msa1186150-mRNA-1:exon:8801;Parent=Msa1186150-mRNA-1
chr8_1	exon	24188039	24188174	24188039	ID=Msa1186150-mRNA-1:exon:8802;Parent=Msa1186150-mRNA-1
chr8_1	exon	24188278	24188451	24188278	ID=Msa1186150-mRNA-1:exon:8803;Parent=Msa1186150-mRNA-1
chr8_1	exon	24188767	24189050	24188767	ID=Msa1186150-mRNA-1:exon:8804;Parent=Msa1186150-mRNA-1
chr8_1	five_prime_UTR	24183814	24183942	24183814	ID=Msa1186150-mRNA-1:five_prime_utr;Parent=Msa1186150-mRNA-1
chr8_1	CDS	24183943	24184521	24183943	ID=Msa1186150-mRNA-1:cds;Parent=Msa1186150-mRNA-1
chr8_1	CDS	24187550	24187702	24187550	ID=Msa1186150-mRNA-1:cds;Parent=Msa1186150-mRNA-1
chr8_1	CDS	24187799	24187881	24187799	ID=Msa1186150-mRNA-1:cds;Parent=Msa1186150-mRNA-1
chr8_1	CDS	24188039	24188174	24188039	ID=Msa1186150-mRNA-1:cds;Parent=Msa1186150-mRNA-1
chr8_1	CDS	24188278	24188451	24188278	ID=Msa1186150-mRNA-1:cds;Parent=Msa1186150-mRNA-1
chr8_1	CDS	24188767	24188940	24188767	ID=Msa1186150-mRNA-1:cds;Parent=Msa1186150-mRNA-1
chr8_1	three_prime_UTR	24188941	24189050	24188941	ID=Msa1186150-mRNA-1:three_prime_utr;Parent=Msa1186150-mRNA-1

Gene Sequence

>Msa1186150

ATGGTTAAAGTTGCAGTGATCGGCGCCGGAGTCTCCGGTATGGTTGCCGCCAAGGAGCTACAGCAAGAAGGCCACAACGTCGTCGTTTTCGAGAAGAACAACCGCATCGGAGGAACTTGGATATACACTCCAAAAACAGACTCCGATCCTCTAAGCATAGACCCAACCAGAGAAACAGTTCACAGCAGTGTATACCTCTCACTACGCACAAATCTTCCAAGACATATCATGGGGTTCTTAGATTACCCTTTGTTCAAGCGAGAATCCGGTGATCCAAGAACGTTTCCGGGTCATGAAGAAGTGCTTCGTTTTTTGGAGGATTTTGCTGGTGAGTTTGGGATACACGGGTTGACCCGATTTGAAACGGAGGTGGTGAAAGTTGAGAGGAAAGGAGGGAAGGAGTGGGTGGTTGAGTCAAGGGGTGGTGACTCAGTGAGTCAAGAAGTGTTTGAAGCGGTTATTGTGTGTTCAGGTCATTTTGTGGAACCGAGACTTGCTTTGGTTCCTGGTATTGAAAACTTTCGTGGCTTTCAAATGCATTCTCATAATTACAGAGTTCCTCATTCTTTCAAAGACCAGGTTGTGATTTTAATTGGGCTAGGATCTAGTTCATTTGACATCTCAAGAGATATTGCAAGACTAGCAAAGGAAGTTCATGTTGCAACAAGACCGAAACCAGATTTGAAGGGTAGGAAGTTAGAAAATGTTCGCAATATATGTTTCCATACTTTGATTAAATGTGTATATGAAGATGGCTTGGTTGCCTTTGAAGATGGATTCTCTACTCATGCGGATGCCATAATTCACTGTACAGGGTACAAATACCACATTCCTTTCCTAGAAACAAATGGAACAGTAACCATTGAAGATAATCGTGTTGGGCCACTATACAAACACGTTTTTCCTCCTTCACTAGCACCATGGCTCTCTTTCATTGGTTTAACCTATAAGGAAATTGCTTTCAGTGTGATTGAGTTACAAGCCAAGTGGATTGCTAGGGTTTTATCTGGTAAGATTCTTTTACCCGATGAAGAAGAGATGATGAAATCTATTAAAGATTTCTATCAATCCATGGAGGAAAATGGGTTACCCAAGCGTCAAACTCATTCGCTTCGTCCATTGCAAGGTGACTACAAACATTGGCTAGTGGAACAGATTGGGCTGCCTCCATTGGAAGATTGGAGGGACAATATGTTGATGGAGTGTTTGAAGAATTCAATTGAAATGAATGAAATGTTTAGAGATGAATGGGATGATAATTATTGGGATGCTATCAGACAGAGTGGATCAGCTTCTTAA

Protein sequence

>Msa1186150

MVKVAVIGAGVSGMVAAKELQQEGHNVVVFEKNNRIGGTWIYTPKTDSDPLSIDPTRETVHSSVYLSLRTNLPRHIMGFLDYPLFKRESGDPRTFPGHEEVLRFLEDFAGEFGIHGLTRFETEVVKVERKGGKEWVVESRGGDSVSQEVFEAVIVCSGHFVEPRLALVPGIENFRGFQMHSHNYRVPHSFKDQVVILIGLGSSSFDISRDIARLAKEVHVATRPKPDLKGRKLENVRNICFHTLIKCVYEDGLVAFEDGFSTHADAIIHCTGYKYHIPFLETNGTVTIEDNRVGPLYKHVFPPSLAPWLSFIGLTYKEIAFSVIELQAKWIARVLSGKILLPDEEEMMKSIKDFYQSMEENGLPKRQTHSLRPLQGDYKHWLVEQIGLPPLEDWRDNMLMECLKNSIEMNEMFRDEWDDNYWDAIRQSGSAS*