AlfalfaGEDB | Msa0045530

M. sativa cultivar zhongmu-4 / Msa0045530

KEGG: K02717
GO: GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009534 GO:0009535 GO:0009536 GO:0009579 GO:0016020 GO:0031976 GO:0031984 GO:0034357 GO:0042651 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464 GO:0055035
PFAM: PF01789.19

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0045530	sp\|O80634\|PNSL1_ARATH	60.084	238	91	2	1	234	1	238	3.69e-96	283

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000180	Msa0045530	0.806430	8.259100e-50	-8.615850e-47
Msa0000220	Msa0045530	0.883212	5.433129e-71	-8.615850e-47
Msa0001040	Msa0045530	0.836742	7.829939e-57	-8.615850e-47
Msa0001420	Msa0045530	0.857397	1.678691e-62	-8.615850e-47
Msa0001510	Msa0045530	0.892852	1.083119e-74	-8.615850e-47
Msa0001540	Msa0045530	0.892996	9.476722e-75	-8.615850e-47
Msa0002060	Msa0045530	0.834114	3.612960e-56	-8.615850e-47
Msa0002690	Msa0045530	0.897539	1.274931e-76	-8.615850e-47
Msa0002700	Msa0045530	0.892717	1.226928e-74	-8.615850e-47
Msa0003340	Msa0045530	0.824325	8.580919e-54	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0045530	MtrunA17_Chr1g0212681	99.573	234	1	0	1	234	1	234	9.68e-178	487
Msa0045530	MtrunA17_Chr7g0222751	38.220	191	117	1	45	234	50	240	6.87e-46	152

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0045530	AT2G39470.1	60.084	238	91	2	1	234	1	238	3.75e-97	283
Msa0045530	AT2G39470.2	60.593	236	85	3	1	234	1	230	2.76e-95	278
Msa0045530	AT2G39470.3	58.824	170	62	3	1	168	1	164	1.43e-60	188
Msa0045530	AT3G55330.1	37.569	181	111	2	53	231	48	228	2.48e-42	144

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Find 83 sgRNAs with CRISPR-Local

Find 138 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACTCCTAGCCACATCACA+AGG	0.625733	1_1:+77471440	Msa0045530:five_prime_UTR
AAATCATGAACACACCTTAT+GGG	0.424094	1_1:-77471584	None:intergenic
AACAATATATGACATGCAAG+AGG	0.615986	1_1:+77472799	Msa0045530:CDS
AAGAAAGATATCAGAGATTT+GGG	0.360869	1_1:+77472633	Msa0045530:CDS
AAGATACTACACATTAATTG+TGG	0.525487	1_1:+77473481	Msa0045530:CDS
AAGTGGTAGCAGACTCCTTT+AGG	0.329582	1_1:+77473546	Msa0045530:CDS
AATGTATGTGTCAAAGTGGG+CGG	0.746612	1_1:-77473728	None:intergenic
ACAAAAGATCGGTATCTGTC+TGG	0.502898	1_1:-77472117	None:intergenic
ACAAAATGCATGGAAGTTAA+GGG	0.508559	1_1:+77474123	Msa0045530:three_prime_UTR
ACACACCTTATGGGATAAGT+AGG	0.562591	1_1:-77471575	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAATCATGAACACACCTTAT+GGG	-	chr1_1:77471587-77471606	None:intergenic	30.0%
AAAATCATGAACACACCTTA+TGG	-	chr1_1:77471588-77471607	None:intergenic	30.0%
CACATTCAAATCATTTGCTT+CGG	+	chr1_1:77471694-77471713	Msa0045530:CDS	30.0%
TGATAAAGGTAGATTACTTC+TGG	-	chr1_1:77471882-77471901	None:intergenic	30.0%
GACAAACTTGTAGAAAATTG+TGG	-	chr1_1:77472500-77472519	None:intergenic	30.0%
GAAGAAAGATATCAGAGATT+TGG	+	chr1_1:77472632-77472651	Msa0045530:CDS	30.0%
TCTTGCATGTCATATATTGT+TGG	-	chr1_1:77472800-77472819	None:intergenic	30.0%
AACAATATATGACATGCAAG+AGG	+	chr1_1:77472799-77472818	Msa0045530:CDS	30.0%
GATTGTTTGTCAATATGCAT+TGG	-	chr1_1:77472891-77472910	None:intergenic	30.0%
AGATTACTTACCATTTCCTA+TGG	-	chr1_1:77473020-77473039	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_1	gene	77471438	77474178	77471438	ID=Msa0045530;Name=Msa0045530
chr1_1	mRNA	77471438	77474178	77471438	ID=Msa0045530-mRNA-1;Parent=Msa0045530;Name=Msa0045530-mRNA-1;_AED=0.10;_eAED=0.10;_QI=100\|1\|1\|1\|1\|1\|8\|595\|234
chr1_1	exon	77471438	77471591	77471438	ID=Msa0045530-mRNA-1:exon:26440;Parent=Msa0045530-mRNA-1
chr1_1	exon	77471684	77471780	77471684	ID=Msa0045530-mRNA-1:exon:26441;Parent=Msa0045530-mRNA-1
chr1_1	exon	77471940	77472023	77471940	ID=Msa0045530-mRNA-1:exon:26442;Parent=Msa0045530-mRNA-1
chr1_1	exon	77472112	77472185	77472112	ID=Msa0045530-mRNA-1:exon:26443;Parent=Msa0045530-mRNA-1
chr1_1	exon	77472546	77472668	77472546	ID=Msa0045530-mRNA-1:exon:26444;Parent=Msa0045530-mRNA-1
chr1_1	exon	77472749	77472820	77472749	ID=Msa0045530-mRNA-1:exon:26445;Parent=Msa0045530-mRNA-1
chr1_1	exon	77472932	77473028	77472932	ID=Msa0045530-mRNA-1:exon:26446;Parent=Msa0045530-mRNA-1
chr1_1	exon	77473480	77474178	77473480	ID=Msa0045530-mRNA-1:exon:26447;Parent=Msa0045530-mRNA-1
chr1_1	five_prime_UTR	77471438	77471537	77471438	ID=Msa0045530-mRNA-1:five_prime_utr;Parent=Msa0045530-mRNA-1
chr1_1	CDS	77471538	77471591	77471538	ID=Msa0045530-mRNA-1:cds;Parent=Msa0045530-mRNA-1
chr1_1	CDS	77471684	77471780	77471684	ID=Msa0045530-mRNA-1:cds;Parent=Msa0045530-mRNA-1
chr1_1	CDS	77471940	77472023	77471940	ID=Msa0045530-mRNA-1:cds;Parent=Msa0045530-mRNA-1
chr1_1	CDS	77472112	77472185	77472112	ID=Msa0045530-mRNA-1:cds;Parent=Msa0045530-mRNA-1
chr1_1	CDS	77472546	77472668	77472546	ID=Msa0045530-mRNA-1:cds;Parent=Msa0045530-mRNA-1
chr1_1	CDS	77472749	77472820	77472749	ID=Msa0045530-mRNA-1:cds;Parent=Msa0045530-mRNA-1
chr1_1	CDS	77472932	77473028	77472932	ID=Msa0045530-mRNA-1:cds;Parent=Msa0045530-mRNA-1
chr1_1	CDS	77473480	77473583	77473480	ID=Msa0045530-mRNA-1:cds;Parent=Msa0045530-mRNA-1
chr1_1	three_prime_UTR	77473584	77474178	77473584	ID=Msa0045530-mRNA-1:three_prime_utr;Parent=Msa0045530-mRNA-1

Gene Sequence

>Msa0045530

ATGGTAGTTTCTTCTTGCTCTTTAACCTGGACTTCATCCTACTTATCCCATAAGCTAAACTTGCCACATTCAAATCATTTGCTTCGGAATACTACAACTTCATCTTCTAACAATGTCTCTTGTGCAATGGAAACAACTTCCAGTACAGAAAGACACTGCCAGAGAAGACCACTGCTGTTGGGTATAGGAGCATTGACAGCAAATTTGTTACCAGCAAACTCACTCTTAGCTGAAGAAATACCAGACAGATACCGATCTTTTGTCGACTATGAAGATGGATATTCTTACATTTATCCCTCAGATTGGAAGGAATTTGACTTCAGGGCACACGATTCTGCATTCAAAGACAGATATTTACAGTTACAAAATGTAAGGGTGAGATTCATACCAACTGAGAAGAAAGATATCAGAGATTTGGGTCCAATGGAAGAGGTTATTACTGATTTGGTGAAACATAGATATGCTGCCCCAAACCAAAGACCAACAATATATGACATGCAAGAGCGGACGATAGATGGAAAACATTATTATACAATGGAATATGTACTTACATCACGAAATTACGCTAGTGCCTCCTTTACGACACTTGCCATAGGAAATGGAAGATACTACACATTAATTGTGGGAGCCAATGAAAGACGATGGAAAAAAGTACGAGATCAGCTTAAAGTGGTAGCAGACTCCTTTAGGCTCCTTGACATCTGA

Protein sequence

>Msa0045530

MVVSSCSLTWTSSYLSHKLNLPHSNHLLRNTTTSSSNNVSCAMETTSSTERHCQRRPLLLGIGALTANLLPANSLLAEEIPDRYRSFVDYEDGYSYIYPSDWKEFDFRAHDSAFKDRYLQLQNVRVRFIPTEKKDIRDLGPMEEVITDLVKHRYAAPNQRPTIYDMQERTIDGKHYYTMEYVLTSRNYASASFTTLAIGNGRYYTLIVGANERRWKKVRDQLKVVADSFRLLDI*