AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0270500


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0270500 MtrunA17_Chr2g0277261 51.639 122 26 2 11 111 142 251 2.09e-25 98.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 27 sgRNAs with CRISPR-Local

Find 120 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCAGTGTCTTTAATATTAG+AGG 0.249899 2_3:+1719911 Msa0270500:CDS
CGTAGTGAAAAGGCGCAAAA+AGG 0.384129 2_3:+1720339 Msa0270500:CDS
GATAGTTCTGATGGAGGTTC+TGG 0.387267 2_3:+1719412 Msa0270500:CDS
GTAGGTTATTCCAAACAAAT+CGG 0.408482 2_3:+1719826 Msa0270500:intron
TTGCATCTTAAAGGTTCAAA+TGG 0.411084 2_3:+1720208 Msa0270500:intron
CAGTATGAGCCCGATTTGTT+TGG 0.414042 2_3:-1719836 None:intergenic
GATTGTGGTACATCGATATC+AGG 0.419626 2_3:+1720280 Msa0270500:CDS
TCCCTTGTCTTCTGTGCAAG+TGG 0.422763 2_3:-1719877 None:intergenic
CAAACAAATCGGGCTCATAC+TGG 0.426550 2_3:+1719837 Msa0270500:CDS
GTTCTGGCAGGACTCGCATT+GGG 0.465963 2_3:+1719428 Msa0270500:CDS
GGTTCTGGCAGGACTCGCAT+TGG 0.494249 2_3:+1719427 Msa0270500:CDS
ATCTTAATCTCGTAGTGAAA+AGG 0.506452 2_3:+1720329 Msa0270500:CDS
CTCCACTTGCACAGAAGACA+AGG 0.508423 2_3:+1719875 Msa0270500:CDS
GGTGACACAGCTGTGTATCA+AGG 0.521115 2_3:+1720301 Msa0270500:CDS
CTCATACTGGCGAATGCGAT+GGG 0.528771 2_3:+1719850 Msa0270500:CDS
TAGGTTATTCCAAACAAATC+GGG 0.537117 2_3:+1719827 Msa0270500:intron
GTTCTGATGGAGGTTCTGGC+AGG 0.540131 2_3:+1719416 Msa0270500:CDS
GCTCATACTGGCGAATGCGA+TGG 0.552483 2_3:+1719849 Msa0270500:CDS
AATATATCTCAAGATGATGC+TGG 0.556632 2_3:+1720253 Msa0270500:CDS
TCAATGCGAGATAGTTCTGA+TGG 0.566163 2_3:+1719403 None:intergenic
GTGTCTTTAATATTAGAGGA+TGG 0.571592 2_3:+1719915 Msa0270500:CDS
TCTTGATAAGAAGTGCAACA+AGG 0.619615 2_3:+1720366 Msa0270500:CDS
CTTGATAAGAAGTGCAACAA+GGG 0.620361 2_3:+1720367 Msa0270500:CDS
GATGATGCTGGTAATGATTG+TGG 0.637016 2_3:+1720265 Msa0270500:CDS
GATAAGAAGTGCAACAAGGG+TGG 0.647056 2_3:+1720370 Msa0270500:CDS
ATGCGAGATAGTTCTGATGG+AGG 0.651636 2_3:+1719406 Msa0270500:CDS
TCCACTTGCACAGAAGACAA+GGG 0.715461 2_3:+1719876 Msa0270500:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAGAAAAATCGAAATATA+AGG - chr2_3:1719771-1719790 None:intergenic 15.0%
!! ATTAGAAAAATCGAAATATA+AGG - chr2_3:1719771-1719790 None:intergenic 15.0%
!!! TTATGTTATTTATCTTTCCA+TGG + chr2_3:1719551-1719570 Msa0270500:intron 20.0%
!!! ATAACGATTAATATTTTTCG+AGG - chr2_3:1719731-1719750 None:intergenic 20.0%
!! AGAAATAAGCAATTATTATC+AGG - chr2_3:1719966-1719985 None:intergenic 20.0%
!!! AATAATTGCTTATTTCTAAG+AGG + chr2_3:1719969-1719988 Msa0270500:intron 20.0%
!!! TTATGTTATTTATCTTTCCA+TGG + chr2_3:1719551-1719570 Msa0270500:intron 20.0%
!!! ATAACGATTAATATTTTTCG+AGG - chr2_3:1719731-1719750 None:intergenic 20.0%
!! AGAAATAAGCAATTATTATC+AGG - chr2_3:1719966-1719985 None:intergenic 20.0%
!!! AATAATTGCTTATTTCTAAG+AGG + chr2_3:1719969-1719988 Msa0270500:intron 20.0%
!!! CATGATAATAATTAGCCTTT+TGG - chr2_3:1719482-1719501 None:intergenic 25.0%
! CTAATTATTATCATGCTATG+TGG + chr2_3:1719487-1719506 Msa0270500:intron 25.0%
! TATTTCACATACATAGTCTT+TGG + chr2_3:1719654-1719673 Msa0270500:intron 25.0%
!! AGCTTTCTTTAAAAAAGAGT+AGG + chr2_3:1719808-1719827 Msa0270500:intron 25.0%
! TTCAGTGTCTTTAATATTAG+AGG + chr2_3:1719911-1719930 Msa0270500:CDS 25.0%
! GTAAACATTCTTTCAAACAT+TGG - chr2_3:1719944-1719963 None:intergenic 25.0%
! GTTTGTATTAATGAATGTGA+TGG + chr2_3:1720022-1720041 Msa0270500:intron 25.0%
!!! CATGATAATAATTAGCCTTT+TGG - chr2_3:1719482-1719501 None:intergenic 25.0%
! CTAATTATTATCATGCTATG+TGG + chr2_3:1719487-1719506 Msa0270500:intron 25.0%
! TATTTCACATACATAGTCTT+TGG + chr2_3:1719654-1719673 Msa0270500:intron 25.0%
!! AGCTTTCTTTAAAAAAGAGT+AGG + chr2_3:1719808-1719827 Msa0270500:intron 25.0%
! TTCAGTGTCTTTAATATTAG+AGG + chr2_3:1719911-1719930 Msa0270500:CDS 25.0%
! GTAAACATTCTTTCAAACAT+TGG - chr2_3:1719944-1719963 None:intergenic 25.0%
! GTTTGTATTAATGAATGTGA+TGG + chr2_3:1720022-1720041 Msa0270500:intron 25.0%
GTAGGTTATTCCAAACAAAT+CGG + chr2_3:1719826-1719845 Msa0270500:intron 30.0%
TAGGTTATTCCAAACAAATC+GGG + chr2_3:1719827-1719846 Msa0270500:intron 30.0%
GTGTCTTTAATATTAGAGGA+TGG + chr2_3:1719915-1719934 Msa0270500:CDS 30.0%
!! GCTTATTTCTAAGAGGAATA+AGG + chr2_3:1719976-1719995 Msa0270500:intron 30.0%
CCTATTATGAATATCCTCTA+GGG - chr2_3:1720050-1720069 None:intergenic 30.0%
CCTAGAGGATATTCATAATA+GGG + chr2_3:1720048-1720067 Msa0270500:intron 30.0%
TTGCATCTTAAAGGTTCAAA+TGG + chr2_3:1720208-1720227 Msa0270500:intron 30.0%
AATATATCTCAAGATGATGC+TGG + chr2_3:1720253-1720272 Msa0270500:CDS 30.0%
ATCTTAATCTCGTAGTGAAA+AGG + chr2_3:1720329-1720348 Msa0270500:CDS 30.0%
GTAGGTTATTCCAAACAAAT+CGG + chr2_3:1719826-1719845 Msa0270500:intron 30.0%
TAGGTTATTCCAAACAAATC+GGG + chr2_3:1719827-1719846 Msa0270500:intron 30.0%
GTGTCTTTAATATTAGAGGA+TGG + chr2_3:1719915-1719934 Msa0270500:CDS 30.0%
!! GCTTATTTCTAAGAGGAATA+AGG + chr2_3:1719976-1719995 Msa0270500:intron 30.0%
CCTATTATGAATATCCTCTA+GGG - chr2_3:1720050-1720069 None:intergenic 30.0%
CCTAGAGGATATTCATAATA+GGG + chr2_3:1720048-1720067 Msa0270500:intron 30.0%
TTGCATCTTAAAGGTTCAAA+TGG + chr2_3:1720208-1720227 Msa0270500:intron 30.0%
AATATATCTCAAGATGATGC+TGG + chr2_3:1720253-1720272 Msa0270500:CDS 30.0%
ATCTTAATCTCGTAGTGAAA+AGG + chr2_3:1720329-1720348 Msa0270500:CDS 30.0%
AACATTGCATTTCACTTCCA+TGG - chr2_3:1719571-1719590 None:intergenic 35.0%
CCCTAGAGGATATTCATAAT+AGG + chr2_3:1720047-1720066 Msa0270500:intron 35.0%
CCCTATTATGAATATCCTCT+AGG - chr2_3:1720051-1720070 None:intergenic 35.0%
!! AAAGTCTTCATTTTGACCAC+TGG - chr2_3:1720098-1720117 None:intergenic 35.0%
GAAGACTTTCTTGATGAATC+AGG + chr2_3:1720109-1720128 Msa0270500:intron 35.0%
TGTGTCAAAGTGGCATTAAA+AGG - chr2_3:1720139-1720158 None:intergenic 35.0%
GAACTGGATTTGCATCTTAA+AGG + chr2_3:1720199-1720218 Msa0270500:intron 35.0%
! TCTTGATAAGAAGTGCAACA+AGG + chr2_3:1720366-1720385 Msa0270500:CDS 35.0%
! CTTGATAAGAAGTGCAACAA+GGG + chr2_3:1720367-1720386 Msa0270500:CDS 35.0%
AACATTGCATTTCACTTCCA+TGG - chr2_3:1719571-1719590 None:intergenic 35.0%
CCCTAGAGGATATTCATAAT+AGG + chr2_3:1720047-1720066 Msa0270500:intron 35.0%
CCCTATTATGAATATCCTCT+AGG - chr2_3:1720051-1720070 None:intergenic 35.0%
!! AAAGTCTTCATTTTGACCAC+TGG - chr2_3:1720098-1720117 None:intergenic 35.0%
GAAGACTTTCTTGATGAATC+AGG + chr2_3:1720109-1720128 Msa0270500:intron 35.0%
TGTGTCAAAGTGGCATTAAA+AGG - chr2_3:1720139-1720158 None:intergenic 35.0%
GAACTGGATTTGCATCTTAA+AGG + chr2_3:1720199-1720218 Msa0270500:intron 35.0%
! TCTTGATAAGAAGTGCAACA+AGG + chr2_3:1720366-1720385 Msa0270500:CDS 35.0%
! CTTGATAAGAAGTGCAACAA+GGG + chr2_3:1720367-1720386 Msa0270500:CDS 35.0%
ACTCGCATTGGGTTTGAAAA+AGG + chr2_3:1719439-1719458 Msa0270500:intron 40.0%
CTCGCATTGGGTTTGAAAAA+GGG + chr2_3:1719440-1719459 Msa0270500:intron 40.0%
!! AAAAAGGGCGTTAAGCAGAT+CGG + chr2_3:1719455-1719474 Msa0270500:intron 40.0%
TAGGGTCACTGCTTCAAATT+TGG + chr2_3:1720066-1720085 Msa0270500:intron 40.0%
AGGGTCACTGCTTCAAATTT+GGG + chr2_3:1720067-1720086 Msa0270500:intron 40.0%
! GATGATGCTGGTAATGATTG+TGG + chr2_3:1720265-1720284 Msa0270500:CDS 40.0%
GATTGTGGTACATCGATATC+AGG + chr2_3:1720280-1720299 Msa0270500:CDS 40.0%
ACTCGCATTGGGTTTGAAAA+AGG + chr2_3:1719439-1719458 Msa0270500:intron 40.0%
CTCGCATTGGGTTTGAAAAA+GGG + chr2_3:1719440-1719459 Msa0270500:intron 40.0%
!! AAAAAGGGCGTTAAGCAGAT+CGG + chr2_3:1719455-1719474 Msa0270500:intron 40.0%
TAGGGTCACTGCTTCAAATT+TGG + chr2_3:1720066-1720085 Msa0270500:intron 40.0%
AGGGTCACTGCTTCAAATTT+GGG + chr2_3:1720067-1720086 Msa0270500:intron 40.0%
! GATGATGCTGGTAATGATTG+TGG + chr2_3:1720265-1720284 Msa0270500:CDS 40.0%
GATTGTGGTACATCGATATC+AGG + chr2_3:1720280-1720299 Msa0270500:CDS 40.0%
!! ATGCGAGATAGTTCTGATGG+AGG + chr2_3:1719406-1719425 Msa0270500:CDS 45.0%
!! GATAGTTCTGATGGAGGTTC+TGG + chr2_3:1719412-1719431 Msa0270500:CDS 45.0%
GTTAAGCAGATCGGTCCAAA+AGG + chr2_3:1719464-1719483 Msa0270500:intron 45.0%
! CAGTATGAGCCCGATTTGTT+TGG - chr2_3:1719839-1719858 None:intergenic 45.0%
CAAACAAATCGGGCTCATAC+TGG + chr2_3:1719837-1719856 Msa0270500:CDS 45.0%
TCCACTTGCACAGAAGACAA+GGG + chr2_3:1719876-1719895 Msa0270500:CDS 45.0%
!! TGAATGTGATGGATCCCTAG+AGG + chr2_3:1720033-1720052 Msa0270500:intron 45.0%
!! CTTCATTTTGACCACTGGAG+TGG - chr2_3:1720093-1720112 None:intergenic 45.0%
CTTCTGCTGATGTGTCAAAG+TGG - chr2_3:1720149-1720168 None:intergenic 45.0%
ACACATCAGCAGAAGACTTC+TGG + chr2_3:1720154-1720173 Msa0270500:intron 45.0%
ACCTCTGTCTGAACATGAAC+TGG + chr2_3:1720183-1720202 Msa0270500:intron 45.0%
TCCAGTTCATGTTCAGACAG+AGG - chr2_3:1720187-1720206 None:intergenic 45.0%
! CGTAGTGAAAAGGCGCAAAA+AGG + chr2_3:1720339-1720358 Msa0270500:CDS 45.0%
! GATAAGAAGTGCAACAAGGG+TGG + chr2_3:1720370-1720389 Msa0270500:CDS 45.0%
!! ATGCGAGATAGTTCTGATGG+AGG + chr2_3:1719406-1719425 Msa0270500:CDS 45.0%
!! GATAGTTCTGATGGAGGTTC+TGG + chr2_3:1719412-1719431 Msa0270500:CDS 45.0%
GTTAAGCAGATCGGTCCAAA+AGG + chr2_3:1719464-1719483 Msa0270500:intron 45.0%
! CAGTATGAGCCCGATTTGTT+TGG - chr2_3:1719839-1719858 None:intergenic 45.0%
CAAACAAATCGGGCTCATAC+TGG + chr2_3:1719837-1719856 Msa0270500:CDS 45.0%
TCCACTTGCACAGAAGACAA+GGG + chr2_3:1719876-1719895 Msa0270500:CDS 45.0%
!! TGAATGTGATGGATCCCTAG+AGG + chr2_3:1720033-1720052 Msa0270500:intron 45.0%
!! CTTCATTTTGACCACTGGAG+TGG - chr2_3:1720093-1720112 None:intergenic 45.0%
CTTCTGCTGATGTGTCAAAG+TGG - chr2_3:1720149-1720168 None:intergenic 45.0%
ACACATCAGCAGAAGACTTC+TGG + chr2_3:1720154-1720173 Msa0270500:intron 45.0%
ACCTCTGTCTGAACATGAAC+TGG + chr2_3:1720183-1720202 Msa0270500:intron 45.0%
TCCAGTTCATGTTCAGACAG+AGG - chr2_3:1720187-1720206 None:intergenic 45.0%
! CGTAGTGAAAAGGCGCAAAA+AGG + chr2_3:1720339-1720358 Msa0270500:CDS 45.0%
! GATAAGAAGTGCAACAAGGG+TGG + chr2_3:1720370-1720389 Msa0270500:CDS 45.0%
CTCATACTGGCGAATGCGAT+GGG + chr2_3:1719850-1719869 Msa0270500:CDS 50.0%
CTCCACTTGCACAGAAGACA+AGG + chr2_3:1719875-1719894 Msa0270500:CDS 50.0%
TCCCTTGTCTTCTGTGCAAG+TGG - chr2_3:1719880-1719899 None:intergenic 50.0%
TCAAATTTGGGCCACTCCAG+TGG + chr2_3:1720079-1720098 Msa0270500:intron 50.0%
GGTGACACAGCTGTGTATCA+AGG + chr2_3:1720301-1720320 Msa0270500:CDS 50.0%
CTCATACTGGCGAATGCGAT+GGG + chr2_3:1719850-1719869 Msa0270500:CDS 50.0%
CTCCACTTGCACAGAAGACA+AGG + chr2_3:1719875-1719894 Msa0270500:CDS 50.0%
TCCCTTGTCTTCTGTGCAAG+TGG - chr2_3:1719880-1719899 None:intergenic 50.0%
TCAAATTTGGGCCACTCCAG+TGG + chr2_3:1720079-1720098 Msa0270500:intron 50.0%
GGTGACACAGCTGTGTATCA+AGG + chr2_3:1720301-1720320 Msa0270500:CDS 50.0%
! GTTCTGATGGAGGTTCTGGC+AGG + chr2_3:1719416-1719435 Msa0270500:CDS 55.0%
GTTCTGGCAGGACTCGCATT+GGG + chr2_3:1719428-1719447 Msa0270500:CDS 55.0%
GCTCATACTGGCGAATGCGA+TGG + chr2_3:1719849-1719868 Msa0270500:CDS 55.0%
! GTTCTGATGGAGGTTCTGGC+AGG + chr2_3:1719416-1719435 Msa0270500:CDS 55.0%
GTTCTGGCAGGACTCGCATT+GGG + chr2_3:1719428-1719447 Msa0270500:CDS 55.0%
GCTCATACTGGCGAATGCGA+TGG + chr2_3:1719849-1719868 Msa0270500:CDS 55.0%
! GGTTCTGGCAGGACTCGCAT+TGG + chr2_3:1719427-1719446 Msa0270500:CDS 60.0%
! GGTTCTGGCAGGACTCGCAT+TGG + chr2_3:1719427-1719446 Msa0270500:CDS 60.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 1719406 1720396 1719406 ID=Msa0270500;Name=Msa0270500
chr2_3 mRNA 1719406 1720396 1719406 ID=Msa0270500-mRNA-1;Parent=Msa0270500;Name=Msa0270500-mRNA-1;_AED=0.46;_eAED=0.51;_QI=0|0|0|1|0|0|3|0|111
chr2_3 exon 1719406 1719449 1719406 ID=Msa0270500-mRNA-1:exon:846;Parent=Msa0270500-mRNA-1
chr2_3 exon 1719830 1719945 1719830 ID=Msa0270500-mRNA-1:exon:847;Parent=Msa0270500-mRNA-1
chr2_3 exon 1720221 1720396 1720221 ID=Msa0270500-mRNA-1:exon:848;Parent=Msa0270500-mRNA-1
chr2_3 CDS 1719406 1719449 1719406 ID=Msa0270500-mRNA-1:cds;Parent=Msa0270500-mRNA-1
chr2_3 CDS 1719830 1719945 1719830 ID=Msa0270500-mRNA-1:cds;Parent=Msa0270500-mRNA-1
chr2_3 CDS 1720221 1720396 1720221 ID=Msa0270500-mRNA-1:cds;Parent=Msa0270500-mRNA-1
Gene Sequence

>Msa0270500

ATGCGAGATAGTTCTGATGGAGGTTCTGGCAGGACTCGCATTGGGTTATTCCAAACAAATCGGGCTCATACTGGCGAATGCGATGGGAACTCCACTTGCACAGAAGACAAGGGAGAAAAAGAGAATTCAGTGTCTTTAATATTAGAGGATGGCAACCAATGTTCAAATGGCTCGCAGAAGACAGTTAGCAGTAATATATCTCAAGATGATGCTGGTAATGATTGTGGTACATCGATATCAGGTGACACAGCTGTGTATCAAGGTGATCATCTTAATCTCGTAGTGAAAAGGCGCAAAAAGGAAAGTCTTGATAAGAAGTGCAACAAGGGTGGTTAA

Protein sequence

>Msa0270500

MRDSSDGGSGRTRIGLFQTNRAHTGECDGNSTCTEDKGEKENSVSLILEDGNQCSNGSQKTVSSNISQDDAGNDCGTSISGDTAVYQGDHLNLVVKRRKKESLDKKCNKGG*