AlfalfaGEDB | Msa0223960

M. sativa cultivar zhongmu-4 / Msa0223960

KEGG: K01183
GO: -
PFAM: PF01535.23

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0223960	XP_003597543.1	79.167	48	10	0	242	289	105	152	1.23e-11	75.1
Msa0223960	XP_003597543.1	93.361	241	11	1	1	241	1	236	3.09e-150	431

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0223960	sp\|Q9STF9\|THA8L_ARATH	34.091	176	104	3	53	225	72	238	3.00e-21	93.6

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0223960	G7IS14	79.167	48	10	0	242	289	105	152	5.90e-12	75.1
Msa0223960	G7IS14	93.361	241	11	1	1	241	1	236	1.48e-150	431

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000490	Msa0223960	-0.824146	9.454917e-54	-8.615850e-47
Msa0003470	Msa0223960	0.803782	2.958443e-49	-8.615850e-47
Msa0006700	Msa0223960	0.813676	2.268616e-51	-8.615850e-47
Msa0006820	Msa0223960	0.814540	1.462336e-51	-8.615850e-47
Msa0008850	Msa0223960	0.832434	9.475953e-56	-8.615850e-47
Msa0017120	Msa0223960	0.803096	4.103631e-49	-8.615850e-47
Msa0017160	Msa0223960	0.808950	2.407075e-50	-8.615850e-47
Msa0020630	Msa0223960	0.824017	1.013823e-53	-8.615850e-47
Msa0027540	Msa0223960	0.809669	1.687506e-50	-8.615850e-47
Msa0037820	Msa0223960	0.800386	1.476470e-48	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0223960	MtrunA17_Chr2g0329741	93.361	241	11	1	1	241	1	236	2.84e-154	431
Msa0223960	MtrunA17_Chr2g0329741	79.167	48	10	0	242	289	105	152	1.14e-15	75.1
Msa0223960	MtrunA17_Chr4g0032231	32.663	199	116	4	38	227	55	244	1.13e-24	99.8
Msa0223960	MtrunA17_Chr1g0160481	27.160	162	109	2	54	212	36	191	6.35e-18	80.9
Msa0223960	MtrunA17_Chr3g0119081	27.160	162	109	2	54	212	40	195	8.49e-18	80.5
Msa0223960	MtrunA17_Chr1g0182521	30.702	114	65	1	130	243	485	584	5.32e-12	66.2
Msa0223960	MtrunA17_Chr3g0143411	29.661	118	82	1	87	204	243	359	7.40e-11	62.4
Msa0223960	MtrunA17_Chr5g0441971	28.472	144	76	4	146	289	29	145	9.60e-11	60.1

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0223960	AT3G46870.1	34.091	176	104	3	53	225	72	238	3.05e-22	93.6
Msa0223960	AT5G09315.1	30.047	213	122	5	45	236	44	250	8.28e-20	87.0
Msa0223960	AT3G46870.2	31.915	188	104	4	53	225	72	250	5.50e-19	85.1
Msa0223960	AT4G39620.2	24.880	209	141	6	23	222	32	233	9.52e-14	71.6
Msa0223960	AT4G39620.1	24.880	209	141	6	23	222	32	233	1.37e-13	71.2
Msa0223960	AT1G62350.1	27.160	162	109	2	54	212	1	156	1.79e-13	68.2
Msa0223960	AT1G62350.2	27.160	162	109	2	54	212	57	212	3.69e-13	68.2
Msa0223960	AT3G18110.1	27.717	184	123	3	118	292	743	925	9.25e-12	66.2
Msa0223960	AT3G04760.1	29.054	148	98	2	87	231	189	332	6.82e-11	63.2
Msa0223960	AT2G15630.1	27.907	172	108	5	130	289	435	602	8.18e-11	62.8

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Find 72 sgRNAs with CRISPR-Local

Find 216 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACAAGAAACCACCTTCAA+TGG	0.451723	2_1:-73690305	Msa0223960:CDS
AAAGAACAAGATGATTGAGA+TGG	0.594568	2_1:-73688191	Msa0223960:CDS
AAAGCAACAACAGTAGTATG+CGG	0.708184	2_1:-73690343	Msa0223960:CDS
AAGATTGTGATTTGGGGTAA+GGG	0.508118	2_1:+73690514	None:intergenic
AAGCACATGGATATTTCAAA+CGG	0.548348	2_1:-73690425	Msa0223960:CDS
AAGGAACAAGATGATTGAGA+TGG	0.616432	2_1:-73686888	Msa0223960:CDS
AATAACTTGAACGCTTTCTT+TGG	0.235310	2_1:+73690276	None:intergenic
AATATAGTGTCAGAATACAA+TGG	0.656425	2_1:+73688223	None:intergenic
AATGAAGGAAGATTGTGATT+TGG	0.352043	2_1:+73690506	None:intergenic
ACAGATCCATGAAGGCTTCA+GGG	0.478380	2_1:-73688051	Msa0223960:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATGAAGGAAGATTGTGATTT+GGG	+	chr2_1:73686880-73686899	None:intergenic	30.0%
AATGAAGGAAGATTGTGATT+TGG	+	chr2_1:73686881-73686900	None:intergenic	30.0%
AAGCACATGGATATTTCAAA+CGG	-	chr2_1:73686959-73686978	Msa0223960:CDS	30.0%
AATAACTTGAACGCTTTCTT+TGG	+	chr2_1:73687111-73687130	None:intergenic	30.0%
TTCTCAAGTCTAAATTGCTT+AGG	-	chr2_1:73687177-73687196	Msa0223960:intron	30.0%
ATCACAATTAAAACCCTGTT+TGG	-	chr2_1:73687278-73687297	Msa0223960:intron	30.0%
ATTAAAACCCTGTTTGGTTA+AGG	-	chr2_1:73687284-73687303	Msa0223960:intron	30.0%
ACAAGTTGCAAACAACTTAT+AGG	+	chr2_1:73687423-73687442	None:intergenic	30.0%
TTCTAATAAGCTATCTAGGA+CGG	-	chr2_1:73687443-73687462	Msa0223960:intron	30.0%
CTACTACAATTGCAAAACTT+AGG	-	chr2_1:73687669-73687688	Msa0223960:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_1	gene	73686835	73690571	73686835	ID=Msa0223960;Name=Msa0223960
chr2_1	mRNA	73686835	73690571	73686835	ID=Msa0223960-mRNA-1;Parent=Msa0223960;Name=Msa0223960-mRNA-1;_AED=0.26;_eAED=0.26;_QI=110\|0.5\|0.66\|1\|1\|1\|3\|0\|294
chr2_1	exon	73686835	73686996	73686835	ID=Msa0223960-mRNA-1:exon:21723;Parent=Msa0223960-mRNA-1
chr2_1	exon	73687904	73688299	73687904	ID=Msa0223960-mRNA-1:exon:21722;Parent=Msa0223960-mRNA-1
chr2_1	exon	73690135	73690571	73690135	ID=Msa0223960-mRNA-1:exon:21721;Parent=Msa0223960-mRNA-1
chr2_1	five_prime_UTR	73690462	73690571	73690462	ID=Msa0223960-mRNA-1:five_prime_utr;Parent=Msa0223960-mRNA-1
chr2_1	CDS	73690135	73690461	73690135	ID=Msa0223960-mRNA-1:cds;Parent=Msa0223960-mRNA-1
chr2_1	CDS	73687904	73688299	73687904	ID=Msa0223960-mRNA-1:cds;Parent=Msa0223960-mRNA-1
chr2_1	CDS	73686835	73686996	73686835	ID=Msa0223960-mRNA-1:cds;Parent=Msa0223960-mRNA-1

Gene Sequence

>Msa0223960

ATGATACTAAGACCAAGCACATGGATATTTCAAACGGAATTGCTTTTCCCACCCTCAAATTCAACCACCACCAACAACAACAACAACAGAAGAAACAAAGCAACAACAGTAGTATGCGGCCTCCGTTCATGCAAAAACAAGAAACCACCTTCAATGGTAATTTCCAAAGAAAGCGTTCAAGTTATTCACGCTCTCAAACTCGCTAAAAATTCCGAAGAAAAACTCAACCAAGTTCTCAAGTCTAAATTGCTTAGGTTATTGAAGCCTGATGTTTTAATTGTCTTGGCTGAATTGCATAGACAAAACCAACTCCATTTATCTCTCAAGGTTTTCGAGTTTATTATCAGTGATGAGGAAGCTGGGTATGATAAATGGCTGCTGCCATTGTATTCTGACACTATATTGTTGCTGGGAAAGAACAAGATGATTGAGATGGCGGAAGAGATGTTCAATCAAGTTGTGGAAAAGGGGTTGAAACCAGATACGAGGTTGTTCAATGAGATGATCGGGGTTTATTTGCAAGTTGGTAATACGGAGAAGGCGATGGAGGTTTACAGATCCATGAAGGCTTCAGGGTGTTTGCCGGATTCATTGACATTCACTATACTGATTAGGAATCTAATGAATAATGGAGAACATGAACTGGTTGAAACTTTGAAAAAAGAGAGTTTTGACTATGTCAATAAACCTGATAAATTTGTTCAGAAAGTCCAGCTGAAACATGTTTTAGAGCTTATTATTAGTGATGAGGAAGCAGGATATGATACATTGCTGGAACAGTTGTATTCTGACATGATACTGTTGTTGGGAAGGAACAAGATGATTGAGATGGCAGAAGAGATGTTCAATCGGGTGGTTGAAACTGGAAACGAGGTTGTTAAATGA

Protein sequence

>Msa0223960

MILRPSTWIFQTELLFPPSNSTTTNNNNNRRNKATTVVCGLRSCKNKKPPSMVISKESVQVIHALKLAKNSEEKLNQVLKSKLLRLLKPDVLIVLAELHRQNQLHLSLKVFEFIISDEEAGYDKWLLPLYSDTILLLGKNKMIEMAEEMFNQVVEKGLKPDTRLFNEMIGVYLQVGNTEKAMEVYRSMKASGCLPDSLTFTILIRNLMNNGEHELVETLKKESFDYVNKPDKFVQKVQLKHVLELIISDEEAGYDTLLEQLYSDMILLLGRNKMIEMAEEMFNRVVETGNEVVK*