Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380012105.01.T01 | XP_003598457.1 | 86.364 | 396 | 49 | 2 | 1 | 396 | 1 | 391 | 0 | 689 |
Co-expression Network
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380012105.01.T01 | MTR_3g013950 | 86.364 | 396 | 49 | 2 | 1 | 396 | 1 | 391 | 0.0 | 689 |
| MsG0380012105.01.T01 | MTR_0267s0050 | 86.111 | 396 | 48 | 3 | 1 | 396 | 1 | 389 | 0.0 | 680 |
| MsG0380012105.01.T01 | MTR_4g037465 | 55.250 | 400 | 157 | 8 | 2 | 396 | 17 | 399 | 3.46e-144 | 416 |
| MsG0380012105.01.T01 | MTR_7g056820 | 56.576 | 403 | 142 | 9 | 2 | 396 | 3 | 380 | 2.83e-143 | 412 |
| MsG0380012105.01.T01 | MTR_2g079810 | 57.353 | 272 | 83 | 5 | 1 | 267 | 1 | 244 | 9.01e-103 | 306 |
| MsG0380012105.01.T01 | MTR_2g079810 | 54.321 | 81 | 33 | 4 | 317 | 396 | 228 | 305 | 2.03e-15 | 76.6 |
| MsG0380012105.01.T01 | MTR_0128s0110 | 65.238 | 210 | 68 | 3 | 191 | 396 | 1 | 209 | 3.59e-89 | 268 |
| MsG0380012105.01.T01 | MTR_7g056290 | 37.470 | 419 | 208 | 15 | 5 | 389 | 21 | 419 | 6.28e-66 | 216 |
| MsG0380012105.01.T01 | MTR_4g024890 | 36.543 | 405 | 219 | 14 | 1 | 391 | 5 | 385 | 4.95e-64 | 210 |
| MsG0380012105.01.T01 | MTR_7g061060 | 37.566 | 378 | 204 | 11 | 3 | 369 | 9 | 365 | 5.71e-64 | 210 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380012105.01.T01 | AT3G06240 | 26.531 | 392 | 216 | 15 | 5 | 362 | 35 | 388 | 7.61e-27 | 111 |
| MsG0380012105.01.T01 | AT3G23880 | 25.897 | 390 | 237 | 12 | 9 | 391 | 17 | 361 | 3.17e-24 | 103 |
| MsG0380012105.01.T01 | AT4G12560 | 26.488 | 336 | 185 | 12 | 9 | 321 | 7 | 303 | 1.40e-20 | 93.2 |
| MsG0380012105.01.T01 | AT4G12560 | 26.488 | 336 | 185 | 12 | 9 | 321 | 7 | 303 | 1.40e-20 | 93.2 |
| MsG0380012105.01.T01 | AT4G12560 | 26.488 | 336 | 185 | 12 | 9 | 321 | 7 | 303 | 1.40e-20 | 93.2 |
| MsG0380012105.01.T01 | AT4G22390 | 25.602 | 332 | 189 | 13 | 9 | 321 | 7 | 299 | 9.19e-16 | 79.0 |
| MsG0380012105.01.T01 | AT4G22390 | 25.602 | 332 | 189 | 13 | 9 | 321 | 7 | 299 | 1.03e-15 | 78.6 |
| MsG0380012105.01.T01 | AT2G02030 | 25.743 | 303 | 185 | 10 | 9 | 292 | 42 | 323 | 8.85e-15 | 75.1 |
| MsG0380012105.01.T01 | AT3G23260 | 24.629 | 337 | 205 | 14 | 2 | 331 | 5 | 299 | 1.24e-11 | 65.9 |
| MsG0380012105.01.T01 | AT2G40910 | 23.123 | 333 | 190 | 15 | 8 | 317 | 71 | 360 | 4.43e-11 | 64.7 |
Find 73 sgRNAs with CRISPR-Local
Find 161 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACAAAATGGGTTCTCATG+TGG | 0.607888 | 3:-10969786 | None:intergenic |
| AAATTATCATTTGTATGAAT+GGG | 0.405683 | 3:+10969612 | MsG0380012105.01.T01:CDS |
| AAATTGGTGAATTCCTTAAT+TGG | 0.248151 | 3:+10969881 | MsG0380012105.01.T01:CDS |
| AACATATTATTATCAGGAGG+CGG | 0.588218 | 3:-10968525 | None:intergenic |
| AAGAAATTTGTACTCAGTTC+TGG | 0.264761 | 3:+10968448 | MsG0380012105.01.T01:CDS |
| AAGTATATGTCGTTGCCAGA+TGG | 0.604075 | 3:+10969694 | MsG0380012105.01.T01:CDS |
| AATCTCGCGAGAGGCTTCAC+CGG | 0.460869 | 3:-10968120 | None:intergenic |
| AATGAGTGAATTATAGTGTT+TGG | 0.354481 | 3:-10968154 | None:intergenic |
| AATTGGCTCTGCTAACGGAT+TGG | 0.483628 | 3:+10968383 | MsG0380012105.01.T01:CDS |
| ACACACCCTAATTAGCTTAG+AGG | 0.326651 | 3:+10968227 | MsG0380012105.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AACATATTATTATCAGGAGG+CGG | - | Chr3:10968528-10968547 | None:intergenic | 30.0% | |
| AACCAATGTGCAATGATAAT+TGG | - | Chr3:10969337-10969356 | None:intergenic | 30.0% | |
| AAGAAATTTGTACTCAGTTC+TGG | + | Chr3:10968448-10968467 | MsG0380012105.01.T01:CDS | 30.0% | |
| AGCTATGTTGATCTTCAAAT+TGG | + | Chr3:10969865-10969884 | MsG0380012105.01.T01:CDS | 30.0% | |
| ATCACAAGATTGTGATACTT+GGG | + | Chr3:10969291-10969310 | MsG0380012105.01.T01:intron | 30.0% | |
| ATTATGAATTGCCTTAGTGA+TGG | + | Chr3:10968790-10968809 | MsG0380012105.01.T01:intron | 30.0% | |
| CAAAAAAAATCACAAAAGGG+TGG | + | Chr3:10968420-10968439 | MsG0380012105.01.T01:CDS | 30.0% | |
| CAAGCGATAAAATCAATAAC+TGG | - | Chr3:10969654-10969673 | None:intergenic | 30.0% | |
| CATTTCCCACAAAACAAAAT+GGG | - | Chr3:10969800-10969819 | None:intergenic | 30.0% | |
| CTTAGAGGAAAACAATACTT+GGG | + | Chr3:10968242-10968261 | MsG0380012105.01.T01:CDS | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 10968066 | 10970092 | 10968066 | ID=MsG0380012105.01;Name=MsG0380012105.01 |
| Chr3 | mRNA | 10968066 | 10970092 | 10968066 | ID=MsG0380012105.01.T01;Parent=MsG0380012105.01;Name=MsG0380012105.01.T01;_AED=0.50;_eAED=0.50;_QI=0|0|0|1|1|1|2|0|396 |
| Chr3 | exon | 10968066 | 10968599 | 10968066 | ID=MsG0380012105.01.T01:exon:228;Parent=MsG0380012105.01.T01 |
| Chr3 | exon | 10969436 | 10970092 | 10969436 | ID=MsG0380012105.01.T01:exon:229;Parent=MsG0380012105.01.T01 |
| Chr3 | CDS | 10968066 | 10968599 | 10968066 | ID=MsG0380012105.01.T01:cds;Parent=MsG0380012105.01.T01 |
| Chr3 | CDS | 10969436 | 10970092 | 10969436 | ID=MsG0380012105.01.T01:cds;Parent=MsG0380012105.01.T01 |
| Gene Sequence |
>MsG0380012105.01.T01 ATGTCTCTTCCAATACTTCTTCCAGAACTCATAGACGACATCATATTGCGGCTTCCGGTGAAGCCTCTCGCGAGATTCAAATGTGTATCCAAACACTATAATTCACTCATTTCCGACCCTGAATTCACAAAACTTCACCTTCAAAGATTACCTAAAAATTCACACACCCTAATTAGCTTAGAGGAAAACAATACTTGGGTTATAACTCCGCACTCAGTGCGTCATTTACTCGAAAACCCGTCATCCATTACCGAAGAAGAAGACGCTAGTCGTCGTTTTAACATGAACGGGAACTATTTTTGGAACTTTTTTGTTTCAATTGGCTCTGCTAACGGATTGGTCTGTTTGATCAGTCAAAAAAAATCACAAAAGGGTGGAAATAAAGAAATTTGTACTCAGTTCTGGAACCCTACTTTACGGTTAAGATCCCAAGACTCACCAAATTTGACTATCATGCCTCCGCCTCCTGATAATAATATGTTGTCCAATGTTCACTTTGGTTTCGGCTATGATGATTCAAGTGACACATACAAGGTGGTAGCTGTGTTTTGGAATTGTATAGCACAAAAGATGGAGGCGAAAGTTCATTGCATGGGCGATAGTTCTTGGAGAAATACTCTTGCTTGCCCTGATTTTCCAATTTTAGTAAAAAGGACTATCGTTGGACCATTCGTGAATGGTAGTGTTAACTGGTTGGCACATCATAACTTAAATTATCATTTGTATGAATGGGAAAATGTTGCCATCAACCAGTTATTGATTTTATCGCTTGATTTGCGAACGGAGACGTGTAAGTATATGTCGTTGCCAGATGGTACAACCGTAGTGCCCCAAGATCTACTAAGCCTTGCTGTTTTGAGGGGTTGCTTGTGTTTTTATTATAACCACATGAGAACCCATTTTGTTTTGTGGGAAATGAAAGAGTTTGGAGTTCAAGAGTCTTGGACTCAGTTGGTGAATGTTAGCTATGTTGATCTTCAAATTGGTGAATTCCTTAATTGGTTGTTGTTGCCAGTGTGCCTATCCGAAAATGGAGATGTCGTGATGCTAGTTTGCGAAGAAGAGTATGATGCTATTATGTATAATCAGAAAGATGGTATTGCTGAACCTTTTGAACTTTCTAACAACCAAATTCGTTATGCTGATGAACATATGCAGAGCTTGGTTTTGCCTTGTCCTCATCCACATTAA |
| Protein sequence |
>MsG0380012105.01.T01 MSLPILLPELIDDIILRLPVKPLARFKCVSKHYNSLISDPEFTKLHLQRLPKNSHTLISLEENNTWVITPHSVRHLLENPSSITEEEDASRRFNMNGNYFWNFFVSIGSANGLVCLISQKKSQKGGNKEICTQFWNPTLRLRSQDSPNLTIMPPPPDNNMLSNVHFGFGYDDSSDTYKVVAVFWNCIAQKMEAKVHCMGDSSWRNTLACPDFPILVKRTIVGPFVNGSVNWLAHHNLNYHLYEWENVAINQLLILSLDLRTETCKYMSLPDGTTVVPQDLLSLAVLRGCLCFYYNHMRTHFVLWEMKEFGVQESWTQLVNVSYVDLQIGEFLNWLLLPVCLSENGDVVMLVCEEEYDAIMYNQKDGIAEPFELSNNQIRYADEHMQSLVLPCPHPH* |
