AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780038976.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780038976.01.T01 MTR_7g066060 90.741 270 20 1 37 301 143 412 3.98e-179 504
MsG0780038976.01.T01 MTR_7g066080 71.429 287 60 3 37 301 134 420 5.56e-143 410
MsG0780038976.01.T01 MTR_6g055210 53.242 293 103 10 37 301 137 423 2.73e-86 266
MsG0780038976.01.T01 MTR_6g016285 51.195 293 108 11 37 301 136 421 2.75e-79 248
MsG0780038976.01.T01 MTR_3g080370 48.110 291 118 8 38 301 137 421 3.30e-75 237
MsG0780038976.01.T01 MTR_6g086920 48.973 292 115 11 38 301 188 473 2.61e-73 234
MsG0780038976.01.T01 MTR_5g056020 48.136 295 119 9 36 301 135 424 4.13e-73 233
MsG0780038976.01.T01 MTR_6g092560 47.195 303 136 12 16 301 104 399 6.04e-72 229
MsG0780038976.01.T01 MTR_0707s0020 47.038 287 114 11 38 301 176 447 2.45e-69 222
MsG0780038976.01.T01 MTR_6g088385 46.713 289 112 11 37 301 52 322 8.33e-66 209
MsG0780038976.01.T01 MTR_8g030630 57.789 199 64 4 118 298 72 268 3.84e-63 206
MsG0780038976.01.T01 MTR_8g030630 58.242 182 52 4 99 276 279 440 6.28e-61 200
MsG0780038976.01.T01 MTR_3g464470 45.230 283 118 9 35 297 116 381 2.75e-57 189
MsG0780038976.01.T01 MTR_4g031020 43.509 285 120 11 36 296 161 428 1.65e-53 180
MsG0780038976.01.T01 MTR_7g078840 42.963 270 127 9 31 277 134 399 4.81e-53 179
MsG0780038976.01.T01 MTR_5g058620 44.304 237 105 8 64 281 25 253 1.70e-52 174
MsG0780038976.01.T01 MTR_1g058870 44.304 237 105 8 64 281 25 253 1.70e-52 174
MsG0780038976.01.T01 MTR_7g066100 39.726 292 132 9 31 296 121 394 2.00e-52 177
MsG0780038976.01.T01 MTR_4g013185 40.550 291 129 9 37 295 147 425 5.59e-50 171
MsG0780038976.01.T01 MTR_4g030980 41.901 284 126 10 36 296 161 428 8.00e-50 171
MsG0780038976.01.T01 MTR_7g078900 43.083 253 125 8 37 277 144 389 8.61e-50 171
MsG0780038976.01.T01 MTR_3g464500 43.956 273 120 10 43 297 147 404 1.66e-49 169
MsG0780038976.01.T01 MTR_4g030090 40.364 275 132 9 42 296 138 400 4.07e-49 168
MsG0780038976.01.T01 MTR_7g066000 42.086 278 124 11 39 294 139 401 6.43e-49 167
MsG0780038976.01.T01 MTR_1g442850 40.876 274 135 9 34 295 117 375 1.36e-48 166
MsG0780038976.01.T01 MTR_4g024890 42.105 266 130 8 42 295 131 384 1.94e-48 166
MsG0780038976.01.T01 MTR_7g070830 39.655 290 139 10 33 295 140 420 2.39e-48 167
MsG0780038976.01.T01 MTR_7g070880 38.966 290 141 10 33 295 140 420 2.80e-48 166
MsG0780038976.01.T01 MTR_7g056710 41.288 264 128 9 39 294 135 379 6.19e-48 164
MsG0780038976.01.T01 MTR_4g030830 41.392 273 130 9 42 296 134 394 6.89e-48 165
MsG0780038976.01.T01 MTR_2g043410 37.086 302 144 10 22 297 137 418 8.11e-48 165
MsG0780038976.01.T01 MTR_7g056503 38.621 290 152 9 22 296 120 398 1.66e-47 164
MsG0780038976.01.T01 MTR_2g043400 37.847 288 132 9 37 297 152 419 1.73e-47 164
MsG0780038976.01.T01 MTR_8g066440 41.455 275 131 10 39 297 143 403 3.37e-47 163
MsG0780038976.01.T01 MTR_6g065510 39.855 276 129 9 39 295 73 330 4.25e-47 161
MsG0780038976.01.T01 MTR_7g055830 39.130 299 135 11 36 297 70 358 6.27e-47 161
MsG0780038976.01.T01 MTR_7g055870 38.095 315 145 12 23 297 122 426 1.11e-46 162
MsG0780038976.01.T01 MTR_4g013180 41.379 261 122 8 36 277 122 370 1.46e-46 163
MsG0780038976.01.T01 MTR_7g055930 38.730 315 143 14 23 297 122 426 1.80e-46 162
MsG0780038976.01.T01 MTR_7g066030 39.298 285 128 11 37 296 139 403 9.71e-46 159
MsG0780038976.01.T01 MTR_7g078860 41.699 259 128 10 40 285 136 384 1.20e-45 159
MsG0780038976.01.T01 MTR_7g077070 35.737 319 155 13 21 295 117 429 3.44e-45 159
MsG0780038976.01.T01 MTR_7g078040 36.486 296 153 12 16 281 113 403 3.88e-45 158
MsG0780038976.01.T01 MTR_7g060940 40.072 277 120 9 39 294 141 392 5.82e-45 157
MsG0780038976.01.T01 MTR_7g061090 40.364 275 132 8 34 295 150 405 9.29e-45 157
MsG0780038976.01.T01 MTR_2g006360 40.075 267 124 8 37 294 122 361 1.12e-44 155
MsG0780038976.01.T01 MTR_1g088120 37.801 291 135 10 20 277 101 378 1.52e-44 156
MsG0780038976.01.T01 MTR_4g013175 38.806 268 134 7 38 287 148 403 3.97e-44 155
MsG0780038976.01.T01 MTR_7g056003 38.194 288 130 10 39 297 141 409 4.12e-44 155
MsG0780038976.01.T01 MTR_0464s0020 38.225 293 136 11 33 297 155 430 4.66e-44 155
MsG0780038976.01.T01 MTR_7g061050 40.511 274 131 10 34 295 130 383 5.78e-44 154
MsG0780038976.01.T01 MTR_7g009265 38.571 280 149 9 36 297 51 325 6.21e-44 152
MsG0780038976.01.T01 MTR_7g057270 39.007 282 146 9 33 295 139 413 8.43e-44 154
MsG0780038976.01.T01 MTR_7g056717 40.149 269 134 9 35 295 136 385 8.90e-44 154
MsG0780038976.01.T01 MTR_4g019990 39.493 276 125 10 43 296 134 389 1.47e-43 153
MsG0780038976.01.T01 MTR_7g055977 39.147 258 142 5 26 277 112 360 1.48e-43 153
MsG0780038976.01.T01 MTR_4g033495 38.596 285 143 9 26 293 133 402 1.80e-43 154
MsG0780038976.01.T01 MTR_7g062060 38.176 296 152 11 14 295 121 399 5.15e-43 152
MsG0780038976.01.T01 MTR_2g047200 38.628 277 137 11 34 296 137 394 7.79e-43 151
MsG0780038976.01.T01 MTR_7g077970 37.793 299 131 13 40 295 135 421 9.01e-43 152
MsG0780038976.01.T01 MTR_1g112010 39.615 260 122 9 42 296 96 325 1.68e-42 149
MsG0780038976.01.T01 MTR_2g461450 39.608 255 135 8 29 278 113 353 2.08e-42 149
MsG0780038976.01.T01 MTR_7g079160 39.773 264 127 10 38 281 153 404 2.26e-42 152
MsG0780038976.01.T01 MTR_6g017305 36.986 292 154 11 27 297 1 283 2.93e-42 147
MsG0780038976.01.T01 MTR_7g091630 36.735 294 144 10 37 297 148 432 3.78e-42 150
MsG0780038976.01.T01 MTR_7g056200 38.462 286 109 11 36 297 141 383 6.62e-42 149
MsG0780038976.01.T01 MTR_3g437520 36.882 263 128 8 39 280 161 406 7.69e-42 150
MsG0780038976.01.T01 MTR_7g056737 39.370 254 132 7 35 282 136 373 1.22e-41 149
MsG0780038976.01.T01 MTR_7g056173 41.129 248 112 9 72 294 27 265 1.31e-41 147
MsG0780038976.01.T01 MTR_7g061060 40.000 265 125 11 34 285 133 376 1.52e-41 148
MsG0780038976.01.T01 MTR_7g056290 35.836 293 140 10 31 296 150 421 1.84e-41 148
MsG0780038976.01.T01 MTR_7g055953 41.350 237 114 8 72 295 90 314 2.34e-41 145
MsG0780038976.01.T01 MTR_7g077990 35.165 273 132 8 23 279 126 369 3.13e-41 147
MsG0780038976.01.T01 MTR_7g034310 43.103 232 103 9 72 295 17 227 4.85e-41 142
MsG0780038976.01.T01 MTR_4g008790 37.984 258 116 6 38 279 186 415 9.05e-41 147
MsG0780038976.01.T01 MTR_7g056390 36.585 287 136 10 35 295 150 416 9.16e-41 146
MsG0780038976.01.T01 MTR_7g028660 37.132 272 144 8 36 295 122 378 2.07e-40 145
MsG0780038976.01.T01 MTR_7g061010 39.636 275 126 10 35 294 137 386 2.77e-40 144
MsG0780038976.01.T01 MTR_5g068040 38.078 281 128 11 39 294 120 379 9.01e-40 143
MsG0780038976.01.T01 MTR_6g017280 39.552 268 147 9 40 297 127 389 1.22e-39 143
MsG0780038976.01.T01 MTR_7g056570 39.847 261 131 9 43 295 140 382 1.56e-39 144
MsG0780038976.01.T01 MTR_3g072470 37.671 292 137 12 31 296 146 418 1.56e-39 143
MsG0780038976.01.T01 MTR_2g010920 36.014 286 152 8 36 295 144 424 2.56e-39 143
MsG0780038976.01.T01 MTR_7g060960 37.457 291 142 12 20 294 100 366 2.66e-39 141
MsG0780038976.01.T01 MTR_7g052150 36.397 272 139 10 22 279 127 378 7.51e-39 141
MsG0780038976.01.T01 MTR_7g052670 35.517 290 144 12 43 301 152 429 7.70e-39 142
MsG0780038976.01.T01 MTR_4g034860 34.146 287 162 8 7 278 118 392 9.52e-39 141
MsG0780038976.01.T01 MTR_7g113500 36.232 276 148 9 39 295 115 381 1.08e-38 140
MsG0780038976.01.T01 MTR_4g006350 35.897 273 138 10 43 294 617 873 2.30e-38 144
MsG0780038976.01.T01 MTR_5g096710 35.231 281 152 8 30 297 137 400 2.46e-38 140
MsG0780038976.01.T01 MTR_5g069760 37.500 272 131 12 38 295 123 369 4.37e-38 138
MsG0780038976.01.T01 MTR_0128s0010 35.495 293 157 10 23 297 121 399 1.19e-37 138
MsG0780038976.01.T01 MTR_7g077910 37.415 294 135 12 34 296 61 336 1.41e-37 136
MsG0780038976.01.T01 MTR_0097s0030 34.812 293 147 9 26 296 118 388 1.83e-37 137
MsG0780038976.01.T01 MTR_2g047240 34.812 293 147 9 26 296 118 388 1.83e-37 137
MsG0780038976.01.T01 MTR_7g055987 39.450 218 100 8 39 250 141 332 4.30e-37 135
MsG0780038976.01.T01 MTR_1g015730 37.681 276 128 10 37 294 144 393 9.63e-37 135
MsG0780038976.01.T01 MTR_7g050480 37.597 258 129 9 31 279 135 369 1.17e-36 135
MsG0780038976.01.T01 MTR_5g043160 33.446 296 159 11 26 296 176 458 2.16e-36 135
MsG0780038976.01.T01 MTR_2g010950 35.273 275 155 8 38 295 136 404 2.41e-36 134
MsG0780038976.01.T01 MTR_7g445890 35.811 296 161 12 25 297 95 384 2.76e-36 134
MsG0780038976.01.T01 MTR_7g050520 37.600 250 125 8 39 279 142 369 4.44e-36 133
MsG0780038976.01.T01 MTR_0128s0070 35.593 295 153 13 21 294 117 395 1.16e-35 132
MsG0780038976.01.T01 MTR_4g129190 36.429 280 141 12 40 295 123 389 1.40e-35 132
MsG0780038976.01.T01 MTR_1g071010 35.689 283 137 12 36 297 130 388 1.59e-35 132
MsG0780038976.01.T01 MTR_7g035295 33.829 269 140 6 40 297 140 381 3.10e-35 131
MsG0780038976.01.T01 MTR_1g029650 36.154 260 128 10 36 279 136 373 4.05e-35 131
MsG0780038976.01.T01 MTR_2g047892 34.432 273 139 9 43 296 146 397 5.12e-35 130
MsG0780038976.01.T01 MTR_3g013470 37.657 239 124 8 51 277 120 345 6.34e-35 130
MsG0780038976.01.T01 MTR_0128s0020 37.175 269 137 10 39 294 143 392 1.88e-34 129
MsG0780038976.01.T01 MTR_7g062100 36.149 296 144 11 16 297 134 398 2.61e-34 129
MsG0780038976.01.T01 MTR_2g047878 35.793 271 126 10 36 296 132 364 2.81e-34 128
MsG0780038976.01.T01 MTR_4g030790 38.835 206 109 6 42 240 105 300 3.15e-34 127
MsG0780038976.01.T01 MTR_6g017295 35.689 283 133 11 40 285 65 335 3.79e-34 130
MsG0780038976.01.T01 MTR_0649s0020 34.304 309 157 12 17 296 97 388 4.26e-34 128
MsG0780038976.01.T01 MTR_4g011660 35.052 291 142 7 39 297 144 419 4.45e-34 129
MsG0780038976.01.T01 MTR_7g078960 33.564 289 147 9 37 297 17 288 5.67e-34 125
MsG0780038976.01.T01 MTR_0242s0060 33.218 289 155 11 19 279 110 388 5.98e-34 128
MsG0780038976.01.T01 MTR_0242s0040 33.218 289 155 11 19 279 110 388 5.98e-34 128
MsG0780038976.01.T01 MTR_7g091640 38.739 222 113 8 23 228 134 348 1.18e-33 127
MsG0780038976.01.T01 MTR_7g056820 33.333 264 141 11 29 278 114 356 1.24e-33 127
MsG0780038976.01.T01 MTR_7g091610 41.530 183 94 5 125 297 69 248 1.39e-33 123
MsG0780038976.01.T01 MTR_2g010900 36.864 236 126 8 38 258 102 329 1.48e-33 127
MsG0780038976.01.T01 MTR_7g066840 33.446 296 154 12 23 297 126 399 7.25e-33 125
MsG0780038976.01.T01 MTR_7g066850 35.117 299 154 10 23 297 124 406 1.03e-32 125
MsG0780038976.01.T01 MTR_4g020000 35.907 259 140 10 53 295 95 343 1.88e-32 123
MsG0780038976.01.T01 MTR_5g069930 34.944 269 143 12 38 294 127 375 2.30e-32 123
MsG0780038976.01.T01 MTR_7g445750 41.624 197 85 6 37 212 143 330 3.22e-32 122
MsG0780038976.01.T01 MTR_4g030760 35.662 272 126 10 42 296 192 431 4.99e-32 123
MsG0780038976.01.T01 MTR_1g015740 36.715 207 99 7 71 277 120 294 1.05e-31 120
MsG0780038976.01.T01 MTR_7g070440 33.566 286 162 10 23 294 99 370 1.16e-31 121
MsG0780038976.01.T01 MTR_6g017270 32.168 286 136 7 19 297 108 342 1.85e-31 120
MsG0780038976.01.T01 MTR_2g437110 34.586 266 139 10 43 296 151 393 5.02e-31 121
MsG0780038976.01.T01 MTR_4g128320 34.252 254 120 10 36 277 39 257 9.74e-31 117
MsG0780038976.01.T01 MTR_0267s0050 33.333 264 153 9 43 294 130 382 1.53e-30 119
MsG0780038976.01.T01 MTR_7g056260 38.278 209 92 9 33 224 129 317 2.04e-30 117
MsG0780038976.01.T01 MTR_7g006360 31.273 275 120 7 24 295 67 275 8.07e-30 115
MsG0780038976.01.T01 MTR_4g037465 31.126 302 166 12 19 299 117 397 9.65e-30 116
MsG0780038976.01.T01 MTR_7g022600 34.138 290 158 11 31 296 142 422 1.17e-29 117
MsG0780038976.01.T01 MTR_2g027820 40.000 180 94 4 125 294 124 299 1.25e-29 114
MsG0780038976.01.T01 MTR_6g042600 41.358 162 81 4 140 294 165 319 5.33e-29 114
MsG0780038976.01.T01 MTR_4g030110 38.372 258 105 10 43 280 155 378 1.14e-28 113
MsG0780038976.01.T01 MTR_1g047490 38.942 208 83 7 23 215 119 297 2.17e-28 111
MsG0780038976.01.T01 MTR_3g013950 31.786 280 166 10 34 299 121 389 2.61e-28 112
MsG0780038976.01.T01 MTR_2g047895 32.472 271 134 10 43 294 66 306 3.38e-28 111
MsG0780038976.01.T01 MTR_7g056760 32.226 301 176 9 15 296 114 405 3.61e-28 112
MsG0780038976.01.T01 MTR_2g017720 37.321 209 103 9 82 279 89 280 5.65e-28 112
MsG0780038976.01.T01 MTR_7g070937 48.855 131 51 4 71 186 11 140 1.74e-27 105
MsG0780038976.01.T01 MTR_4g129160 36.515 241 118 10 34 256 137 360 7.81e-27 108
MsG0780038976.01.T01 MTR_6g081280 36.100 241 119 9 72 295 18 240 8.21e-27 105
MsG0780038976.01.T01 MTR_7g056797 33.456 272 154 9 39 296 145 403 8.26e-27 108
MsG0780038976.01.T01 MTR_7g022670 38.372 172 101 3 128 296 182 351 1.28e-26 107
MsG0780038976.01.T01 MTR_7g058630 37.500 168 98 4 135 297 16 181 1.58e-26 103
MsG0780038976.01.T01 MTR_2g010935 32.197 264 136 8 45 295 16 249 2.36e-26 104
MsG0780038976.01.T01 MTR_0128s0110 34.211 190 99 6 110 283 10 189 1.96e-25 101
MsG0780038976.01.T01 MTR_7g061940 37.379 206 105 8 73 270 78 267 9.15e-25 100
MsG0780038976.01.T01 MTR_2g021150 30.888 259 152 9 42 295 132 368 1.12e-23 99.8
MsG0780038976.01.T01 MTR_5g069950 33.846 195 97 6 38 227 33 200 1.05e-22 94.0
MsG0780038976.01.T01 MTR_3g055000 40.845 142 64 4 158 295 1 126 1.90e-22 90.9
MsG0780038976.01.T01 MTR_7g070900 38.953 172 59 4 72 228 237 377 2.91e-22 96.7
MsG0780038976.01.T01 MTR_5g011350 38.824 170 81 7 39 203 95 246 2.99e-22 93.6
MsG0780038976.01.T01 MTR_7g066010 30.634 284 121 11 37 296 139 370 3.78e-22 95.5
MsG0780038976.01.T01 MTR_7g445580 43.860 114 57 2 165 278 40 146 3.93e-22 90.5
MsG0780038976.01.T01 MTR_2g060555 31.852 270 136 14 38 292 45 281 1.20e-21 92.8
MsG0780038976.01.T01 MTR_8g030660 44.444 126 40 4 38 134 143 267 1.58e-21 92.0
MsG0780038976.01.T01 MTR_7g066040 39.181 171 84 5 36 187 45 214 6.56e-21 89.7
MsG0780038976.01.T01 MTR_7g078880 32.189 233 118 8 85 300 93 302 1.38e-20 91.3
MsG0780038976.01.T01 MTR_7g056023 37.245 196 90 9 26 205 99 277 1.82e-19 86.7
MsG0780038976.01.T01 MTR_7g056793 28.929 280 139 11 23 294 3 230 9.52e-18 80.9
MsG0780038976.01.T01 MTR_7g078940 38.650 163 73 7 38 183 177 329 1.01e-17 83.2
MsG0780038976.01.T01 MTR_3g055070 37.398 123 59 3 158 279 1 106 7.93e-17 76.6
MsG0780038976.01.T01 MTR_4g024910 43.434 99 49 2 183 278 151 245 8.66e-17 79.7
MsG0780038976.01.T01 MTR_2g046920 30.357 224 138 7 72 295 167 372 4.33e-16 78.2
MsG0780038976.01.T01 MTR_2g029990 29.137 278 152 14 12 279 141 383 9.55e-16 77.4
MsG0780038976.01.T01 MTR_7g089830 38.393 112 51 2 150 257 31 128 1.28e-15 73.2
MsG0780038976.01.T01 MTR_6g007135 29.119 261 159 13 42 295 148 389 1.61e-14 73.6
MsG0780038976.01.T01 MTR_2g021190 33.482 224 114 12 61 273 148 347 3.28e-14 72.8
MsG0780038976.01.T01 MTR_7g062070 33.884 121 72 4 161 277 75 191 3.22e-13 67.8
MsG0780038976.01.T01 MTR_5g022420 27.600 250 139 12 42 282 140 356 3.47e-13 69.7
MsG0780038976.01.T01 MTR_7g060930 43.421 76 42 1 135 210 4 78 1.13e-12 62.8
MsG0780038976.01.T01 MTR_7g058660 36.111 108 62 3 191 291 103 210 1.34e-12 66.2
MsG0780038976.01.T01 MTR_7g013590 27.076 277 158 11 42 295 148 403 1.83e-12 67.8
MsG0780038976.01.T01 MTR_2g021155 29.536 237 140 11 42 273 147 361 2.06e-12 68.2
MsG0780038976.01.T01 MTR_3g011020 35.802 162 84 7 72 224 157 307 2.47e-12 67.0
MsG0780038976.01.T01 MTR_1g055375 28.205 234 145 10 70 295 148 366 6.96e-12 65.9
MsG0780038976.01.T01 MTR_8g101580 28.137 263 158 11 40 295 156 394 6.99e-12 65.9
MsG0780038976.01.T01 MTR_1g016960 40.777 103 54 3 199 294 152 254 1.81e-11 63.5
MsG0780038976.01.T01 MTR_3g007270 30.864 162 98 6 66 224 195 345 2.86e-11 63.9
MsG0780038976.01.T01 MTR_8g062660 31.980 197 102 9 42 222 135 315 2.87e-11 64.3
MsG0780038976.01.T01 MTR_1g094210 29.183 257 149 10 42 295 172 398 3.53e-11 63.9
MsG0780038976.01.T01 MTR_1g097490 27.679 224 123 8 40 256 160 351 3.99e-11 63.5
MsG0780038976.01.T01 MTR_3g009240 28.814 236 128 14 72 295 175 382 5.03e-11 63.2
MsG0780038976.01.T01 MTR_1g094210 29.570 186 109 6 42 226 172 336 5.11e-11 63.2
MsG0780038976.01.T01 MTR_4g063955 53.571 56 23 1 37 92 144 196 5.62e-11 62.4
MsG0780038976.01.T01 MTR_3g010870 26.545 275 145 15 40 295 148 384 9.14e-11 62.4
MsG0780038976.01.T01 MTR_8g467140 29.592 196 125 6 40 232 142 327 9.48e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 50 sgRNAs with CRISPR-Local

Find 112 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAGAACTGAATTTGTTATT+TGG 0.177270 7:+56274039 MsG0780038976.01.T01:CDS
AACAATTTCCCTTTGATTCT+TGG 0.202118 7:+56273795 MsG0780038976.01.T01:CDS
GCCGACTCTTCAAGTTCTTT+TGG 0.235443 7:+56273989 MsG0780038976.01.T01:CDS
ACCTTGATATTGGATTATTA+TGG 0.243363 7:+56274206 MsG0780038976.01.T01:CDS
GAATGGATATACCTTGATAT+TGG 0.281147 7:+56274196 MsG0780038976.01.T01:CDS
ACAATTTCCCTTTGATTCTT+GGG 0.342230 7:+56273796 MsG0780038976.01.T01:CDS
GTTCTCATACGTTGAAAGTT+TGG 0.343689 7:+56274304 MsG0780038976.01.T01:CDS
GAAGCTTCGAATTTCGCTTT+TGG 0.357538 7:+56273648 MsG0780038976.01.T01:CDS
ATTTGGAAAATGAATGAGTT+TGG 0.357698 7:+56274056 MsG0780038976.01.T01:CDS
GAAGGGTAGCTTCTTTCATC+TGG 0.367877 7:-56273714 None:intergenic
TGGACCCGACTATTTAGAAT+TGG 0.373458 7:+56274092 MsG0780038976.01.T01:CDS
GGTACAAGATCTGCACCATT+TGG 0.399323 7:+56273588 MsG0780038976.01.T01:CDS
GCAGATCTTGTACCGGTGGC+AGG 0.402745 7:-56273579 None:intergenic
AAATTCGAAGCTTCTGATAC+AGG 0.426885 7:-56273639 None:intergenic
AACTGGAACTTACAAGGTGC+TGG 0.440029 7:+56273683 MsG0780038976.01.T01:CDS
ACATGGCACCTCATCAAAAT+CGG 0.457027 7:-56273961 None:intergenic
CTACCCTTCATTGAAAAGTA+AGG 0.459873 7:+56273728 MsG0780038976.01.T01:CDS
TGGTGTCAACTTCGACTCAT+TGG 0.470216 7:+56274324 MsG0780038976.01.T01:CDS
GTACAAGATCTGCACCATTT+GGG 0.471526 7:+56273589 MsG0780038976.01.T01:CDS
AGTTTGGAGTTCGAGAGTCT+TGG 0.476988 7:+56274072 MsG0780038976.01.T01:CDS
GTTTGGATGATGATGATTGT+TGG 0.478496 7:+56273763 MsG0780038976.01.T01:CDS
AGTATCCAATTCTAAATAGT+CGG 0.484507 7:-56274097 None:intergenic
CCATTGTATCTCTCTAAGAA+TGG 0.484620 7:+56274179 MsG0780038976.01.T01:CDS
CATGCATGATAGAGGAAGAA+AGG 0.488203 7:+56272806 MsG0780038976.01.T01:CDS
TAAGGTCAGAGTTCTCAGTT+TGG 0.504870 7:+56273746 MsG0780038976.01.T01:CDS
TTGTCTCGGTCGAAAGATCA+AGG 0.509537 7:-56273917 None:intergenic
GTATCCAATTCTAAATAGTC+GGG 0.510319 7:-56274096 None:intergenic
TCAAGTAATTATCCATTGGA+AGG 0.512609 7:-56274139 None:intergenic
TCCATAATAATCCAATATCA+AGG 0.526404 7:-56274207 None:intergenic
CACTATTCCCAAGAATCAAA+GGG 0.527455 7:-56273803 None:intergenic
TAGTCAAATCGAGTCCCAAA+TGG 0.546744 7:-56273603 None:intergenic
GAATTCAAGTAATTATCCAT+TGG 0.550684 7:-56274143 None:intergenic
GAACTTCTTACAATCTATGT+CGG 0.554151 7:+56274282 MsG0780038976.01.T01:CDS
ACACTATTCCCAAGAATCAA+AGG 0.556855 7:-56273804 None:intergenic
AAGGGTAGCTTCTTTCATCT+GGG 0.559277 7:-56273713 None:intergenic
TGAAGAGTCGGCTGAAAACA+TGG 0.562215 7:-56273978 None:intergenic
TCCAAAAGAACTTGAAGAGT+CGG 0.571916 7:-56273990 None:intergenic
CAGATCTTGTACCGGTGGCA+GGG 0.578307 7:-56273578 None:intergenic
TATATATGGAACCCTGCCAC+CGG 0.580165 7:+56273567 MsG0780038976.01.T01:CDS
ATCATAAGAATCAACAGCCA+AGG 0.586293 7:-56273853 None:intergenic
TGAAATGCATAACCTTCCAA+TGG 0.587162 7:+56274127 MsG0780038976.01.T01:CDS
AAATGGTGCAGATCTTGTAC+CGG 0.602238 7:-56273586 None:intergenic
TTCTGATACAGGCTCACGAA+GGG 0.603221 7:-56273628 None:intergenic
GGCACCTCATCAAAATCGGA+AGG 0.618800 7:-56273957 None:intergenic
TGGTGCAGATCTTGTACCGG+TGG 0.627240 7:-56273583 None:intergenic
CCATTCTTAGAGAGATACAA+TGG 0.636271 7:-56274179 None:intergenic
AATAGTGTCGTGCATTTGAG+TGG 0.644803 7:+56273819 MsG0780038976.01.T01:CDS
AATCGTGCGAAAAGCAAATG+TGG 0.655018 7:-56274013 None:intergenic
TGTCTCGGTCGAAAGATCAA+GGG 0.656326 7:-56273916 None:intergenic
CTTCTGATACAGGCTCACGA+AGG 0.657446 7:-56273629 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAATATAATATTTTTTTAA+TGG - Chr7:56273152-56273171 None:intergenic 0.0%
!!! CAAAAATTAATATATTGTTT+TGG + Chr7:56273023-56273042 MsG0780038976.01.T01:intron 10.0%
!!! AATTGTTTTTTGAAAATCAA+GGG + Chr7:56273067-56273086 MsG0780038976.01.T01:intron 15.0%
!!! TAATTGTTTTTTGAAAATCA+AGG + Chr7:56273066-56273085 MsG0780038976.01.T01:intron 15.0%
!! GAAAGAAGAAAATCATTTAT+TGG + Chr7:56272910-56272929 MsG0780038976.01.T01:intron 20.0%
!!! ATTGTTTTTTGAAAATCAAG+GGG + Chr7:56273068-56273087 MsG0780038976.01.T01:intron 20.0%
! ACCTTGATATTGGATTATTA+TGG + Chr7:56274206-56274225 MsG0780038976.01.T01:CDS 25.0%
! AGAAAATCATTTATTGGAAG+TGG + Chr7:56272916-56272935 MsG0780038976.01.T01:intron 25.0%
! AGTATCCAATTCTAAATAGT+CGG - Chr7:56274100-56274119 None:intergenic 25.0%
! ATATTATTTACCCGATTCAT+AGG + Chr7:56272991-56273010 MsG0780038976.01.T01:intron 25.0%
! ATTATAGTGTAAGCTATCAA+AGG - Chr7:56273238-56273257 None:intergenic 25.0%
! ATTTGGAAAATGAATGAGTT+TGG + Chr7:56274056-56274075 MsG0780038976.01.T01:CDS 25.0%
! GAATTCAAGTAATTATCCAT+TGG - Chr7:56274146-56274165 None:intergenic 25.0%
! TATTATTTACCCGATTCATA+GGG + Chr7:56272992-56273011 MsG0780038976.01.T01:intron 25.0%
! TCCATAATAATCCAATATCA+AGG - Chr7:56274210-56274229 None:intergenic 25.0%
!! TTTTAATGGAATATTGTAGC+TGG - Chr7:56273138-56273157 None:intergenic 25.0%
!!! AGAGAACTGAATTTGTTATT+TGG + Chr7:56274039-56274058 MsG0780038976.01.T01:CDS 25.0%
!!! ATGAAGATGATTACTTTTGT+TGG + Chr7:56272843-56272862 MsG0780038976.01.T01:CDS 25.0%
!!! TTAGGTACTGGTTTTATATA+TGG + Chr7:56273553-56273572 MsG0780038976.01.T01:intron 25.0%
AACAATTTCCCTTTGATTCT+TGG + Chr7:56273795-56273814 MsG0780038976.01.T01:CDS 30.0%
ACAATTTCCCTTTGATTCTT+GGG + Chr7:56273796-56273815 MsG0780038976.01.T01:CDS 30.0%
AGATTCACGAAGAAAAAACA+AGG + Chr7:56272770-56272789 MsG0780038976.01.T01:CDS 30.0%
ATCAAAAACATGCATGATAG+AGG + Chr7:56272798-56272817 MsG0780038976.01.T01:CDS 30.0%
GAACTTCTTACAATCTATGT+CGG + Chr7:56274282-56274301 MsG0780038976.01.T01:CDS 30.0%
GAATGGATATACCTTGATAT+TGG + Chr7:56274196-56274215 MsG0780038976.01.T01:CDS 30.0%
GTATCCAATTCTAAATAGTC+GGG - Chr7:56274099-56274118 None:intergenic 30.0%
TACGCACTATAAGAAGAATT+CGG - Chr7:56273317-56273336 None:intergenic 30.0%
TATGCTTTCTGTACAATACT+TGG + Chr7:56273508-56273527 MsG0780038976.01.T01:intron 30.0%
TCAAGTAATTATCCATTGGA+AGG - Chr7:56274142-56274161 None:intergenic 30.0%
TTCTGTACAATACTTGGAAT+GGG + Chr7:56273514-56273533 MsG0780038976.01.T01:intron 30.0%
TTTCTGTACAATACTTGGAA+TGG + Chr7:56273513-56273532 MsG0780038976.01.T01:intron 30.0%
!! TGACCTTACTTTTCAATGAA+GGG - Chr7:56273734-56273753 None:intergenic 30.0%
!! TTTGGTTGTGACATTTTAAC+TGG + Chr7:56273666-56273685 MsG0780038976.01.T01:CDS 30.0%
!!! CATTTTAACTGGAACTTACA+AGG + Chr7:56273677-56273696 MsG0780038976.01.T01:CDS 30.0%
AAATTCGAAGCTTCTGATAC+AGG - Chr7:56273642-56273661 None:intergenic 35.0%
ACACTATTCCCAAGAATCAA+AGG - Chr7:56273807-56273826 None:intergenic 35.0%
ACAGTCACGGAAAAAGTAAA+CGG - Chr7:56273431-56273450 None:intergenic 35.0%
ATCATAAGAATCAACAGCCA+AGG - Chr7:56273856-56273875 None:intergenic 35.0%
CAAAAATTGCATGGTTGTCT+CGG - Chr7:56273934-56273953 None:intergenic 35.0%
CACTATTCCCAAGAATCAAA+GGG - Chr7:56273806-56273825 None:intergenic 35.0%
CCATTCTTAGAGAGATACAA+TGG - Chr7:56274182-56274201 None:intergenic 35.0%
CTACCCTTCATTGAAAAGTA+AGG + Chr7:56273728-56273747 MsG0780038976.01.T01:CDS 35.0%
GTTCTCATACGTTGAAAGTT+TGG + Chr7:56274304-56274323 MsG0780038976.01.T01:CDS 35.0%
TCCAAAAGAACTTGAAGAGT+CGG - Chr7:56273993-56274012 None:intergenic 35.0%
TGAAATGCATAACCTTCCAA+TGG + Chr7:56274127-56274146 MsG0780038976.01.T01:CDS 35.0%
! CCATTGTATCTCTCTAAGAA+TGG + Chr7:56274179-56274198 MsG0780038976.01.T01:CDS 35.0%
! CTTTCTTTTTCTCTCGATGA+AGG + Chr7:56272946-56272965 MsG0780038976.01.T01:intron 35.0%
! GTTTGGATGATGATGATTGT+TGG + Chr7:56273763-56273782 MsG0780038976.01.T01:CDS 35.0%
!! CTGACCTTACTTTTCAATGA+AGG - Chr7:56273735-56273754 None:intergenic 35.0%
!! TTACTTTTGTTGGAGACGAA+AGG + Chr7:56272853-56272872 MsG0780038976.01.T01:CDS 35.0%
AAATGGTGCAGATCTTGTAC+CGG - Chr7:56273589-56273608 None:intergenic 40.0%
AACAATTAGCTCCATGCGAT+TGG - Chr7:56273473-56273492 None:intergenic 40.0%
AAGAAGAATTCGGTACGTAC+AGG - Chr7:56273307-56273326 None:intergenic 40.0%
AAGGGTAGCTTCTTTCATCT+GGG - Chr7:56273716-56273735 None:intergenic 40.0%
AATACTTGGAATGGGAGGAA+TGG + Chr7:56273522-56273541 MsG0780038976.01.T01:intron 40.0%
AATAGTGTCGTGCATTTGAG+TGG + Chr7:56273819-56273838 MsG0780038976.01.T01:CDS 40.0%
AATCGTGCGAAAAGCAAATG+TGG - Chr7:56274016-56274035 None:intergenic 40.0%
ACATGGCACCTCATCAAAAT+CGG - Chr7:56273964-56273983 None:intergenic 40.0%
AGAAGAATTCGGTACGTACA+GGG - Chr7:56273306-56273325 None:intergenic 40.0%
ATACTTGGAATGGGAGGAAT+GGG + Chr7:56273523-56273542 MsG0780038976.01.T01:intron 40.0%
CATGCATGATAGAGGAAGAA+AGG + Chr7:56272806-56272825 MsG0780038976.01.T01:CDS 40.0%
GATTGGAATTCATACCACCA+CGG - Chr7:56273456-56273475 None:intergenic 40.0%
GGAAGGCAACAAAAATTGCA+TGG - Chr7:56273943-56273962 None:intergenic 40.0%
GGAGCTAATTGTTCTTGCAA+CGG + Chr7:56273480-56273499 MsG0780038976.01.T01:intron 40.0%
GTACAAGATCTGCACCATTT+GGG + Chr7:56273589-56273608 MsG0780038976.01.T01:CDS 40.0%
TAAGGTCAGAGTTCTCAGTT+TGG + Chr7:56273746-56273765 MsG0780038976.01.T01:CDS 40.0%
TAGTCAAATCGAGTCCCAAA+TGG - Chr7:56273606-56273625 None:intergenic 40.0%
TGACCATAGTCACATATTCG+CGG - Chr7:56273376-56273395 None:intergenic 40.0%
TGGTATGAATTCCAATCGCA+TGG + Chr7:56273459-56273478 MsG0780038976.01.T01:intron 40.0%
TGTACAATACTTGGAATGGG+AGG + Chr7:56273517-56273536 MsG0780038976.01.T01:intron 40.0%
TGTTTCAGCTCCCTATGAAT+CGG - Chr7:56273005-56273024 None:intergenic 40.0%
! GAAGCTTCGAATTTCGCTTT+TGG + Chr7:56273648-56273667 MsG0780038976.01.T01:CDS 40.0%
! TGGACCCGACTATTTAGAAT+TGG + Chr7:56274092-56274111 MsG0780038976.01.T01:CDS 40.0%
AACTGGAACTTACAAGGTGC+TGG + Chr7:56273683-56273702 MsG0780038976.01.T01:CDS 45.0%
CACATATTCGCGGTTCTCTT+CGG - Chr7:56273366-56273385 None:intergenic 45.0%
GAACCGCGAATATGTGACTA+TGG + Chr7:56273370-56273389 MsG0780038976.01.T01:intron 45.0%
GAAGAATTCGGTACGTACAG+GGG - Chr7:56273305-56273324 None:intergenic 45.0%
GAAGGGTAGCTTCTTTCATC+TGG - Chr7:56273717-56273736 None:intergenic 45.0%
GCTGGATGTGTCAAATACGA+TGG - Chr7:56273120-56273139 None:intergenic 45.0%
GGTACAAGATCTGCACCATT+TGG + Chr7:56273588-56273607 MsG0780038976.01.T01:CDS 45.0%
GTTTCAGCTCCCTATGAATC+GGG - Chr7:56273004-56273023 None:intergenic 45.0%
TATATATGGAACCCTGCCAC+CGG + Chr7:56273567-56273586 MsG0780038976.01.T01:CDS 45.0%
TGAAGAGTCGGCTGAAAACA+TGG - Chr7:56273981-56274000 None:intergenic 45.0%
TGATTCTCCAGCAAACGAAC+TGG - Chr7:56273407-56273426 None:intergenic 45.0%
TGTCTCGGTCGAAAGATCAA+GGG - Chr7:56273919-56273938 None:intergenic 45.0%
TTCTGATACAGGCTCACGAA+GGG - Chr7:56273631-56273650 None:intergenic 45.0%
TTGTCTCGGTCGAAAGATCA+AGG - Chr7:56273920-56273939 None:intergenic 45.0%
! AGTTTGGAGTTCGAGAGTCT+TGG + Chr7:56274072-56274091 MsG0780038976.01.T01:CDS 45.0%
! ATGGGAGGTATGTTAGGTAC+TGG + Chr7:56273541-56273560 MsG0780038976.01.T01:intron 45.0%
! TGGTGTCAACTTCGACTCAT+TGG + Chr7:56274324-56274343 MsG0780038976.01.T01:CDS 45.0%
!! GCCGACTCTTCAAGTTCTTT+TGG + Chr7:56273989-56274008 MsG0780038976.01.T01:CDS 45.0%
!! GTTGCCTTCCGATTTTGATG+AGG + Chr7:56273953-56273972 MsG0780038976.01.T01:CDS 45.0%
CTCCAGCAAACGAACTGGTA+AGG - Chr7:56273402-56273421 None:intergenic 50.0%
CTTCTGATACAGGCTCACGA+AGG - Chr7:56273632-56273651 None:intergenic 50.0%
GACCTTACCAGTTCGTTTGC+TGG + Chr7:56273397-56273416 MsG0780038976.01.T01:intron 50.0%
GGCACCTCATCAAAATCGGA+AGG - Chr7:56273960-56273979 None:intergenic 50.0%
! GGAGGAATGGGAGGTATGTT+AGG + Chr7:56273535-56273554 MsG0780038976.01.T01:intron 50.0%
! GTTCTCTTCGGTTTGCGGTA+CGG - Chr7:56273354-56273373 None:intergenic 50.0%
! TTCTCTTCGGTTTGCGGTAC+GGG - Chr7:56273353-56273372 None:intergenic 50.0%
! TTTTTCCGTGACTGTCACCG+TGG + Chr7:56273436-56273455 MsG0780038976.01.T01:intron 50.0%
!! GAGTGGCACTTTTTGCTCCT+TGG + Chr7:56273836-56273855 MsG0780038976.01.T01:CDS 50.0%
CTTGGAATGGGAGGAATGGG+AGG + Chr7:56273526-56273545 MsG0780038976.01.T01:intron 55.0%
TACCACCACGGTGACAGTCA+CGG - Chr7:56273444-56273463 None:intergenic 55.0%
TGGTGCAGATCTTGTACCGG+TGG - Chr7:56273586-56273605 None:intergenic 55.0%
! CAGATCTTGTACCGGTGGCA+GGG - Chr7:56273581-56273600 None:intergenic 55.0%
! CGTACAGGGGTATGCAGCTT+CGG - Chr7:56273292-56273311 None:intergenic 55.0%
! TCGCGGTTCTCTTCGGTTTG+CGG - Chr7:56273359-56273378 None:intergenic 55.0%
! TTCGGTTTGCGGTACGGGTA+CGG - Chr7:56273348-56273367 None:intergenic 55.0%
TATGCAGCTTCGGCACGCGT+CGG - Chr7:56273282-56273301 None:intergenic 60.0%
TTCCGTGACTGTCACCGTGG+TGG + Chr7:56273439-56273458 MsG0780038976.01.T01:intron 60.0%
! GCAGATCTTGTACCGGTGGC+AGG - Chr7:56273582-56273601 None:intergenic 60.0%
! TCGGTTTGCGGTACGGGTAC+GGG - Chr7:56273347-56273366 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr7 gene 56272765 56274352 56272765 ID=MsG0780038976.01;Name=MsG0780038976.01
Chr7 mRNA 56272765 56274352 56272765 ID=MsG0780038976.01.T01;Parent=MsG0780038976.01;Name=MsG0780038976.01.T01;_AED=0.43;_eAED=0.43;_QI=0|0|0|0.5|1|1|2|0|301
Chr7 exon 56272765 56272874 56272765 ID=MsG0780038976.01.T01:exon:7889;Parent=MsG0780038976.01.T01
Chr7 exon 56273557 56274352 56273557 ID=MsG0780038976.01.T01:exon:7890;Parent=MsG0780038976.01.T01
Chr7 CDS 56272765 56272874 56272765 ID=MsG0780038976.01.T01:cds;Parent=MsG0780038976.01.T01
Chr7 CDS 56273557 56274352 56273557 ID=MsG0780038976.01.T01:cds;Parent=MsG0780038976.01.T01
Gene Sequence

>MsG0780038976.01.T01

ATGAAAGATTCACGAAGAAAAAACAAGGAGTTAATCAAAAACATGCATGATAGAGGAAGAAAGGAGTGTGCTGTCTTGATGAAGATGATTACTTTTGTTGGAGACGAAAGGTACTGGTTTTATATATGGAACCCTGCCACCGGTACAAGATCTGCACCATTTGGGACTCGATTTGACTATTCCCTTCGTGAGCCTGTATCAGAAGCTTCGAATTTCGCTTTTGGTTGTGACATTTTAACTGGAACTTACAAGGTGCTGGAGTTTCGCCCAGATGAAAGAAGCTACCCTTCATTGAAAAGTAAGGTCAGAGTTCTCAGTTTGGATGATGATGATTGTTGGAGAAATATTAACAATTTCCCTTTGATTCTTGGGAATAGTGTCGTGCATTTGAGTGGCACTTTTTGCTCCTTGGCTGTTGATTCTTATGATTACGAGACTATTACTCATGTTAATCAATTCATGATTGTTTCCCTTGATCTTTCGACCGAGACAACCATGCAATTTTTGTTGCCTTCCGATTTTGATGAGGTGCCATGTTTTCAGCCGACTCTTCAAGTTCTTTTGGACCACATTTGCTTTTCGCACGATTTCAAGAGAACTGAATTTGTTATTTGGAAAATGAATGAGTTTGGAGTTCGAGAGTCTTGGACCCGACTATTTAGAATTGGATACTTTGATCTTGAAATGCATAACCTTCCAATGGATAATTACTTGAATTCTACATTGTTGATGCCATTGTATCTCTCTAAGAATGGATATACCTTGATATTGGATTATTATGGAGATGATGAAGCAGTTATCTATGATCAAAGAGAAAATAGAGCAGAGAGAATTAGAACTTCTTACAATCTATGTCGGTTCTCATACGTTGAAAGTTTGGTGTCAACTTCGACTCATTGGAAGTAA
Protein sequence

>MsG0780038976.01.T01

MKDSRRKNKELIKNMHDRGRKECAVLMKMITFVGDERYWFYIWNPATGTRSAPFGTRFDYSLREPVSEASNFAFGCDILTGTYKVLEFRPDERSYPSLKSKVRVLSLDDDDCWRNINNFPLILGNSVVHLSGTFCSLAVDSYDYETITHVNQFMIVSLDLSTETTMQFLLPSDFDEVPCFQPTLQVLLDHICFSHDFKRTEFVIWKMNEFGVRESWTRLFRIGYFDLEMHNLPMDNYLNSTLLMPLYLSKNGYTLILDYYGDDEAVIYDQRENRAERIRTSYNLCRFSYVESLVSTSTHWK*