AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780039709.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780039709.01.T01 MTR_7g077990 72.414 232 42 2 1 232 78 287 1.51e-114 334
MsG0780039709.01.T01 MTR_7g077910 65.665 233 60 4 6 232 1 219 4.33e-96 285
MsG0780039709.01.T01 MTR_7g078840 55.602 241 88 9 3 232 82 314 2.46e-69 219
MsG0780039709.01.T01 MTR_7g078040 53.191 235 87 6 3 227 82 303 1.08e-68 218
MsG0780039709.01.T01 MTR_7g077070 52.869 244 83 8 6 232 82 310 8.54e-67 213
MsG0780039709.01.T01 MTR_7g079160 50.413 242 94 9 10 232 85 319 1.34e-59 195
MsG0780039709.01.T01 MTR_7g077970 53.153 222 89 7 11 227 81 292 1.36e-58 191
MsG0780039709.01.T01 MTR_0242s0060 50.893 224 91 7 9 227 81 290 9.90e-57 186
MsG0780039709.01.T01 MTR_0242s0040 50.893 224 91 7 9 227 81 290 9.90e-57 186
MsG0780039709.01.T01 MTR_7g078900 48.560 243 84 9 7 232 86 304 1.17e-55 184
MsG0780039709.01.T01 MTR_1g088120 47.177 248 100 8 7 238 70 302 4.86e-55 182
MsG0780039709.01.T01 MTR_7g078860 49.351 231 82 9 11 232 87 291 1.59e-53 177
MsG0780039709.01.T01 MTR_4g013185 45.935 246 92 9 7 232 84 308 3.54e-53 177
MsG0780039709.01.T01 MTR_4g013175 45.902 244 103 8 3 232 80 308 8.46e-53 176
MsG0780039709.01.T01 MTR_4g013180 51.515 198 75 6 45 232 102 288 2.07e-52 176
MsG0780039709.01.T01 MTR_7g078940 50.442 226 83 11 11 221 117 328 2.79e-50 169
MsG0780039709.01.T01 MTR_4g008790 46.781 233 83 6 10 232 129 330 7.91e-50 169
MsG0780039709.01.T01 MTR_3g013470 46.930 228 83 7 7 232 69 260 4.42e-46 157
MsG0780039709.01.T01 MTR_2g043410 41.702 235 104 5 11 232 87 301 4.82e-46 159
MsG0780039709.01.T01 MTR_7g056003 45.652 230 101 7 10 232 81 293 8.97e-46 157
MsG0780039709.01.T01 MTR_6g055210 43.515 239 100 9 11 232 85 305 2.35e-45 157
MsG0780039709.01.T01 MTR_7g056260 45.378 238 101 7 1 232 73 287 3.60e-44 151
MsG0780039709.01.T01 MTR_7g056290 40.756 238 106 7 11 232 88 306 5.26e-44 153
MsG0780039709.01.T01 MTR_7g056390 40.833 240 107 8 9 232 82 302 8.25e-44 152
MsG0780039709.01.T01 MTR_2g043400 42.979 235 99 8 12 232 89 302 2.08e-43 152
MsG0780039709.01.T01 MTR_7g055987 43.644 236 88 9 7 232 81 281 3.31e-43 150
MsG0780039709.01.T01 MTR_3g072470 41.102 236 106 6 11 232 86 302 1.33e-42 149
MsG0780039709.01.T01 MTR_3g464470 43.719 199 90 6 40 232 94 276 4.63e-42 147
MsG0780039709.01.T01 MTR_7g057270 41.102 236 109 9 11 232 90 309 5.65e-42 148
MsG0780039709.01.T01 MTR_7g078960 49.239 197 55 8 52 232 1 168 7.82e-42 144
MsG0780039709.01.T01 MTR_4g128320 52.096 167 65 7 64 227 41 195 1.19e-41 143
MsG0780039709.01.T01 MTR_6g016285 43.154 241 98 10 12 232 84 305 1.01e-40 145
MsG0780039709.01.T01 MTR_7g056503 40.000 230 118 7 11 232 80 297 1.27e-40 144
MsG0780039709.01.T01 MTR_7g055870 40.889 225 111 6 24 232 97 315 4.95e-40 143
MsG0780039709.01.T01 MTR_5g058620 54.676 139 55 3 96 232 31 163 5.57e-40 139
MsG0780039709.01.T01 MTR_1g058870 54.676 139 55 3 96 232 31 163 5.57e-40 139
MsG0780039709.01.T01 MTR_7g066100 39.837 246 119 7 12 242 75 306 1.17e-39 141
MsG0780039709.01.T01 MTR_3g437520 40.945 254 100 11 7 232 86 317 2.06e-39 142
MsG0780039709.01.T01 MTR_7g055930 40.444 225 112 6 24 232 97 315 6.38e-39 140
MsG0780039709.01.T01 MTR_3g464500 40.171 234 103 8 11 232 91 299 7.69e-39 139
MsG0780039709.01.T01 MTR_7g091630 40.336 238 111 9 9 232 94 314 8.62e-39 140
MsG0780039709.01.T01 MTR_0464s0020 38.372 258 98 9 7 232 86 314 3.56e-38 138
MsG0780039709.01.T01 MTR_7g060940 37.931 261 100 10 12 244 86 312 5.83e-38 137
MsG0780039709.01.T01 MTR_7g066040 43.147 197 92 9 45 227 25 215 8.19e-38 132
MsG0780039709.01.T01 MTR_0649s0020 39.241 237 110 11 10 232 71 287 2.20e-37 135
MsG0780039709.01.T01 MTR_7g062060 36.207 232 122 5 12 232 87 303 2.52e-37 135
MsG0780039709.01.T01 MTR_5g056020 39.516 248 112 10 11 232 80 315 2.57e-37 137
MsG0780039709.01.T01 MTR_0097s0030 39.904 208 100 5 31 232 94 282 2.79e-37 135
MsG0780039709.01.T01 MTR_2g047240 39.904 208 100 5 31 232 94 282 2.79e-37 135
MsG0780039709.01.T01 MTR_4g031020 40.385 208 84 6 45 232 137 324 9.91e-37 134
MsG0780039709.01.T01 MTR_2g047200 39.574 235 108 9 11 232 83 296 1.74e-36 133
MsG0780039709.01.T01 MTR_2g047892 41.327 196 88 7 46 232 119 296 2.40e-36 132
MsG0780039709.01.T01 MTR_7g070830 38.281 256 111 10 9 232 80 320 3.76e-36 132
MsG0780039709.01.T01 MTR_3g080370 39.004 241 110 8 11 232 83 305 8.47e-36 132
MsG0780039709.01.T01 MTR_4g030980 40.097 207 86 7 45 232 137 324 1.76e-35 130
MsG0780039709.01.T01 MTR_2g047895 36.957 230 117 9 13 232 11 222 2.49e-35 129
MsG0780039709.01.T01 MTR_7g061090 39.241 237 104 10 12 232 94 306 6.21e-35 129
MsG0780039709.01.T01 MTR_8g066440 38.211 246 108 8 10 232 84 308 1.18e-34 128
MsG0780039709.01.T01 MTR_7g091640 39.149 235 125 8 5 232 90 313 3.12e-34 126
MsG0780039709.01.T01 MTR_5g068040 42.408 191 90 8 47 232 100 275 3.82e-34 126
MsG0780039709.01.T01 MTR_1g442850 39.485 233 108 10 12 232 65 276 5.39e-34 126
MsG0780039709.01.T01 MTR_7g066850 38.889 234 117 9 3 232 79 290 6.07e-34 127
MsG0780039709.01.T01 MTR_7g055830 43.258 178 82 5 68 232 76 247 6.21e-34 125
MsG0780039709.01.T01 MTR_7g070880 37.500 256 113 10 9 232 80 320 1.11e-33 126
MsG0780039709.01.T01 MTR_5g011350 37.083 240 114 11 11 243 38 247 2.07e-33 121
MsG0780039709.01.T01 MTR_6g065510 43.169 183 73 6 59 232 67 227 2.10e-33 123
MsG0780039709.01.T01 MTR_4g030830 41.538 195 88 8 46 232 111 287 2.75e-33 124
MsG0780039709.01.T01 MTR_7g055953 51.799 139 53 5 94 232 86 210 2.91e-33 122
MsG0780039709.01.T01 MTR_2g010950 37.719 228 123 9 14 232 83 300 2.93e-33 124
MsG0780039709.01.T01 MTR_4g019990 36.364 242 113 9 8 232 70 287 4.73e-33 124
MsG0780039709.01.T01 MTR_0707s0020 40.952 210 92 9 41 232 149 344 8.19e-33 124
MsG0780039709.01.T01 MTR_4g030110 43.750 192 85 7 40 223 125 301 9.23e-33 123
MsG0780039709.01.T01 MTR_7g028660 37.288 236 112 8 12 232 71 285 1.01e-32 122
MsG0780039709.01.T01 MTR_7g061060 38.095 231 109 9 13 232 78 285 1.11e-32 123
MsG0780039709.01.T01 MTR_6g086920 40.952 210 92 9 41 232 161 356 1.16e-32 124
MsG0780039709.01.T01 MTR_7g061050 36.975 238 104 9 13 233 75 283 1.38e-32 122
MsG0780039709.01.T01 MTR_7g050480 39.827 231 103 9 11 232 84 287 3.88e-32 121
MsG0780039709.01.T01 MTR_1g029650 38.618 246 100 11 10 238 78 289 4.21e-32 121
MsG0780039709.01.T01 MTR_7g050520 39.394 231 104 9 11 232 84 287 9.94e-32 120
MsG0780039709.01.T01 MTR_7g060960 38.839 224 108 8 12 232 72 269 1.62e-31 119
MsG0780039709.01.T01 MTR_4g034860 37.395 238 111 9 13 232 91 308 1.83e-31 119
MsG0780039709.01.T01 MTR_7g445750 34.298 242 128 6 9 232 83 311 3.23e-31 117
MsG0780039709.01.T01 MTR_7g066000 35.776 232 128 8 9 232 85 303 4.12e-31 119
MsG0780039709.01.T01 MTR_1g071010 35.270 241 112 9 10 232 75 289 4.56e-31 118
MsG0780039709.01.T01 MTR_5g043160 41.206 199 96 7 43 232 164 350 4.92e-31 119
MsG0780039709.01.T01 MTR_2g010900 34.728 239 131 9 7 232 39 265 4.94e-31 118
MsG0780039709.01.T01 MTR_7g445890 42.222 180 81 7 45 215 87 252 1.19e-30 117
MsG0780039709.01.T01 MTR_4g033495 36.481 233 119 6 12 232 88 303 1.45e-30 117
MsG0780039709.01.T01 MTR_7g078880 55.172 116 44 3 111 223 93 203 1.90e-30 117
MsG0780039709.01.T01 MTR_7g066080 39.914 233 103 11 17 232 85 297 1.90e-30 117
MsG0780039709.01.T01 MTR_2g010920 35.685 241 124 10 11 232 92 320 1.99e-30 117
MsG0780039709.01.T01 MTR_7g070937 48.148 135 61 4 96 225 10 140 2.00e-30 111
MsG0780039709.01.T01 MTR_2g461450 38.342 193 92 5 40 232 101 266 4.03e-30 115
MsG0780039709.01.T01 MTR_4g129160 37.179 234 118 9 11 232 84 300 4.83e-30 115
MsG0780039709.01.T01 MTR_7g066030 37.607 234 123 5 11 232 83 305 5.18e-30 115
MsG0780039709.01.T01 MTR_5g069760 37.179 234 115 10 7 232 62 271 1.87e-29 114
MsG0780039709.01.T01 MTR_6g017295 35.268 224 124 7 17 232 13 223 1.96e-29 115
MsG0780039709.01.T01 MTR_7g056023 37.719 228 116 9 11 232 56 263 2.59e-29 111
MsG0780039709.01.T01 MTR_4g030090 38.034 234 115 10 10 232 76 290 3.55e-29 113
MsG0780039709.01.T01 MTR_7g066060 39.506 243 115 11 3 232 81 304 5.17e-29 114
MsG0780039709.01.T01 MTR_7g113500 36.564 227 120 8 13 232 68 277 5.72e-29 112
MsG0780039709.01.T01 MTR_7g056737 36.207 232 113 10 11 232 81 287 1.23e-28 112
MsG0780039709.01.T01 MTR_7g037670 37.674 215 96 10 13 221 73 255 2.23e-28 110
MsG0780039709.01.T01 MTR_4g129190 35.865 237 115 10 12 232 68 283 5.85e-28 110
MsG0780039709.01.T01 MTR_7g056710 35.526 228 119 7 11 232 77 282 6.19e-28 110
MsG0780039709.01.T01 MTR_7g066010 36.726 226 102 7 11 215 83 288 1.84e-27 108
MsG0780039709.01.T01 MTR_5g069930 40.201 199 85 11 45 232 101 276 2.07e-27 108
MsG0780039709.01.T01 MTR_1g015730 37.768 233 91 9 9 238 99 280 2.43e-27 108
MsG0780039709.01.T01 MTR_7g055977 39.738 229 110 9 11 232 69 276 2.53e-27 108
MsG0780039709.01.T01 MTR_4g024890 34.934 229 119 10 12 232 74 280 4.30e-27 107
MsG0780039709.01.T01 MTR_7g062100 38.362 232 101 11 4 221 79 282 5.28e-27 107
MsG0780039709.01.T01 MTR_4g006350 33.047 233 126 10 10 232 560 772 6.07e-27 109
MsG0780039709.01.T01 MTR_4g030790 37.885 227 106 11 20 232 49 254 9.39e-27 106
MsG0780039709.01.T01 MTR_7g056717 36.957 230 114 10 11 232 81 287 1.49e-26 106
MsG0780039709.01.T01 MTR_6g017280 36.224 196 110 5 41 232 99 283 2.27e-26 105
MsG0780039709.01.T01 MTR_7g009265 36.224 196 113 6 41 232 27 214 2.75e-26 104
MsG0780039709.01.T01 MTR_6g088385 40.000 185 77 6 64 232 53 219 2.76e-26 104
MsG0780039709.01.T01 MTR_7g066840 38.053 226 108 11 15 232 93 294 3.05e-26 105
MsG0780039709.01.T01 MTR_7g056760 34.468 235 121 9 11 232 89 303 7.01e-26 104
MsG0780039709.01.T01 MTR_0128s0020 37.768 233 87 11 4 215 78 273 2.46e-25 103
MsG0780039709.01.T01 MTR_7g052150 37.931 203 94 7 32 232 118 290 2.98e-25 102
MsG0780039709.01.T01 MTR_6g017305 38.095 168 93 5 64 230 13 170 3.01e-25 100
MsG0780039709.01.T01 MTR_0128s0010 33.475 236 122 9 7 230 80 292 3.88e-25 102
MsG0780039709.01.T01 MTR_7g061010 37.131 237 118 6 12 242 87 298 4.10e-25 102
MsG0780039709.01.T01 MTR_7g056170 31.174 247 123 6 11 218 133 371 5.51e-25 102
MsG0780039709.01.T01 MTR_5g096710 37.374 198 95 8 41 227 115 294 6.48e-25 102
MsG0780039709.01.T01 MTR_7g061100 36.797 231 97 12 20 238 66 259 2.99e-24 99.0
MsG0780039709.01.T01 MTR_7g056200 35.417 192 80 5 46 230 122 276 4.03e-24 99.8
MsG0780039709.01.T01 MTR_2g047878 34.694 196 89 6 41 232 108 268 5.86e-24 99.0
MsG0780039709.01.T01 MTR_6g092560 36.538 208 89 10 45 233 107 290 6.00e-24 99.8
MsG0780039709.01.T01 MTR_1g047490 35.391 243 93 10 9 232 78 275 6.21e-24 98.2
MsG0780039709.01.T01 MTR_7g070900 46.377 138 63 5 85 216 224 356 7.00e-24 99.8
MsG0780039709.01.T01 MTR_7g056173 46.154 143 63 4 96 232 25 159 8.23e-24 97.8
MsG0780039709.01.T01 MTR_4g011660 33.466 251 117 9 10 232 85 313 1.25e-23 98.6
MsG0780039709.01.T01 MTR_7g056820 33.036 224 125 8 12 232 77 278 1.58e-23 97.8
MsG0780039709.01.T01 MTR_1g046600 44.000 125 56 3 10 129 124 239 1.78e-23 96.7
MsG0780039709.01.T01 MTR_1g112010 33.488 215 103 5 16 228 46 222 2.64e-23 96.7
MsG0780039709.01.T01 MTR_4g037465 33.476 233 116 12 7 226 80 286 4.14e-23 97.1
MsG0780039709.01.T01 MTR_7g034310 43.885 139 60 3 95 232 14 135 4.39e-23 94.0
MsG0780039709.01.T01 MTR_8g101940 40.120 167 73 6 45 204 102 248 1.43e-22 95.1
MsG0780039709.01.T01 MTR_7g022600 33.865 251 123 11 7 232 85 317 1.51e-22 95.5
MsG0780039709.01.T01 MTR_0128s0070 36.123 227 98 10 4 215 82 276 1.62e-22 95.5
MsG0780039709.01.T01 MTR_7g056570 34.468 235 113 9 11 232 77 283 1.79e-22 95.5
MsG0780039709.01.T01 MTR_6g081280 43.902 123 61 3 96 216 16 132 1.98e-21 89.7
MsG0780039709.01.T01 MTR_7g056797 36.170 235 117 10 10 232 88 301 1.07e-20 90.1
MsG0780039709.01.T01 MTR_2g017720 42.636 129 55 4 107 232 88 200 3.72e-20 89.0
MsG0780039709.01.T01 MTR_7g070440 33.790 219 106 10 42 243 92 288 9.67e-20 87.4
MsG0780039709.01.T01 MTR_7g030040 34.872 195 68 8 43 232 123 263 1.17e-19 86.7
MsG0780039709.01.T01 MTR_7g052670 33.333 207 103 8 45 227 124 319 3.62e-19 86.3
MsG0780039709.01.T01 MTR_3g007270 26.522 230 141 6 7 233 116 320 4.83e-19 85.5
MsG0780039709.01.T01 MTR_3g009240 34.637 179 77 8 46 214 127 275 7.85e-19 84.7
MsG0780039709.01.T01 MTR_2g006360 35.450 189 82 6 45 232 111 260 1.23e-18 84.0
MsG0780039709.01.T01 MTR_7g035295 29.204 226 110 6 17 232 85 270 1.61e-18 84.0
MsG0780039709.01.T01 MTR_1g050542 30.500 200 108 6 12 211 117 285 5.58e-18 82.4
MsG0780039709.01.T01 MTR_8g030660 34.737 190 74 9 17 171 93 267 6.39e-17 78.2
MsG0780039709.01.T01 MTR_4g030760 38.365 159 68 9 45 196 174 309 7.93e-17 79.3
MsG0780039709.01.T01 MTR_1g097560 27.273 220 122 7 11 230 99 280 8.50e-17 79.0
MsG0780039709.01.T01 MTR_4g024870 35.838 173 82 6 45 206 142 296 8.80e-17 79.3
MsG0780039709.01.T01 MTR_2g021150 33.488 215 109 10 16 226 85 269 1.55e-16 78.2
MsG0780039709.01.T01 MTR_0267s0050 29.717 212 124 7 10 210 66 263 2.09e-16 78.2
MsG0780039709.01.T01 MTR_3g009300 33.511 188 90 8 46 227 98 256 1.32e-15 75.5
MsG0780039709.01.T01 MTR_3g024130 31.111 180 88 7 39 210 54 205 2.25e-15 74.3
MsG0780039709.01.T01 MTR_3g007430 33.146 178 87 8 44 212 131 285 5.42e-15 73.9
MsG0780039709.01.T01 MTR_7g091610 47.297 74 36 1 162 232 69 142 5.60e-15 72.4
MsG0780039709.01.T01 MTR_1g054575 29.500 200 110 5 12 211 114 282 5.64e-15 74.3
MsG0780039709.01.T01 MTR_4g006840 33.735 166 66 7 44 204 91 217 5.78e-15 72.0
MsG0780039709.01.T01 MTR_4g020000 36.806 144 76 3 96 234 111 244 5.94e-15 73.6
MsG0780039709.01.T01 MTR_7g061940 43.860 114 45 5 100 212 79 174 6.81e-15 72.4
MsG0780039709.01.T01 MTR_3g007330 30.366 191 102 7 46 230 136 301 1.27e-14 72.8
MsG0780039709.01.T01 MTR_2g027820 48.101 79 39 1 162 238 124 202 1.81e-14 71.6
MsG0780039709.01.T01 MTR_8g467140 30.476 210 102 9 11 211 93 267 2.33e-14 71.6
MsG0780039709.01.T01 MTR_2g079810 29.126 206 111 8 10 210 71 246 4.38e-14 70.9
MsG0780039709.01.T01 MTR_8g030630 40.000 110 37 2 123 232 279 359 6.85e-14 70.9
MsG0780039709.01.T01 MTR_8g030630 44.211 95 40 3 141 232 72 156 3.63e-13 68.6
MsG0780039709.01.T01 MTR_6g017270 33.577 137 77 4 17 145 78 208 9.43e-14 70.1
MsG0780039709.01.T01 MTR_2g010935 35.135 148 67 4 86 232 26 145 1.68e-13 68.6
MsG0780039709.01.T01 MTR_1g094210 29.412 221 115 9 12 230 123 304 1.93e-13 69.3
MsG0780039709.01.T01 MTR_1g094210 29.412 221 115 9 12 230 123 304 2.05e-13 69.3
MsG0780039709.01.T01 MTR_5g007610 30.286 175 96 6 45 210 133 290 7.41e-13 67.8
MsG0780039709.01.T01 MTR_3g014540 31.156 199 98 8 43 235 119 284 7.82e-13 67.8
MsG0780039709.01.T01 MTR_3g009310 29.648 199 108 7 41 234 133 304 1.41e-12 67.0
MsG0780039709.01.T01 MTR_2g021190 31.658 199 93 8 45 233 116 281 1.97e-12 66.2
MsG0780039709.01.T01 MTR_1g097330 28.440 218 101 8 10 214 74 249 3.05e-12 65.9
MsG0780039709.01.T01 MTR_3g013740 32.000 175 89 8 43 210 145 296 4.54e-12 65.5
MsG0780039709.01.T01 MTR_2g046920 29.907 214 118 10 17 230 95 276 4.88e-12 65.1
MsG0780039709.01.T01 MTR_6g065980 29.480 173 82 9 46 215 109 244 1.34e-11 63.9
MsG0780039709.01.T01 MTR_3g009260 30.939 181 87 9 41 214 126 275 1.43e-11 63.9
MsG0780039709.01.T01 MTR_3g009290 32.927 164 81 6 46 205 120 258 1.84e-11 62.8
MsG0780039709.01.T01 MTR_2g029990 34.302 172 75 9 45 210 148 287 1.98e-11 63.5
MsG0780039709.01.T01 MTR_7g056560 56.604 53 22 1 165 216 129 181 3.96e-11 61.2
MsG0780039709.01.T01 MTR_2g046800 31.176 170 85 6 44 211 139 278 3.96e-11 62.8
MsG0780039709.01.T01 MTR_3g006670 29.609 179 93 10 45 216 144 296 4.20e-11 62.4
MsG0780039709.01.T01 MTR_0044s0300 30.723 166 86 6 46 210 136 273 6.59e-11 62.0
MsG0780039709.01.T01 MTR_7g407070 32.609 184 90 8 43 216 131 290 7.02e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780039709.01.T01 AT3G23880 33.088 136 67 5 41 172 108 223 6.97e-11 62.0

Find 53 sgRNAs with CRISPR-Local

Find 65 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGGATACGGGTTCCGTTTA+TGG 0.143925 7:-67907696 MsG0780039709.01.T01:CDS
TTGCTTCTGGATTGATGTTC+TGG 0.203838 7:+67907529 None:intergenic
AGCTCCAATTGTTGATGTTT+TGG 0.207396 7:-67907224 MsG0780039709.01.T01:CDS
GTAATTGTTTCACTTGATTT+GGG 0.226123 7:-67907304 MsG0780039709.01.T01:CDS
TGTAATTGTTTCACTTGATT+TGG 0.237944 7:-67907305 MsG0780039709.01.T01:CDS
ATCTGTTTGTCAACTCTCTT+TGG 0.240729 7:-67907724 MsG0780039709.01.T01:CDS
ACATCAACAATTGGAGCTTT+AGG 0.271331 7:+67907229 None:intergenic
TCTTTAGTTGTCTTGGTGGC+TGG 0.301615 7:+67907670 None:intergenic
ACGGGACACCACCACTTTAT+AGG 0.304065 7:+67907558 None:intergenic
GGTTGATTATGTACTTGATT+TGG 0.313946 7:-67907878 MsG0780039709.01.T01:CDS
TCACTTGCACTATAATATTA+AGG 0.322153 7:-67907347 MsG0780039709.01.T01:CDS
GTTGATTATGTACTTGATTT+GGG 0.326434 7:-67907877 MsG0780039709.01.T01:CDS
TAAGTTTGCGTGATAATGTT+TGG 0.330397 7:-67907495 MsG0780039709.01.T01:CDS
ATTTGAGCGGCACTCTTAAT+TGG 0.358907 7:-67907384 MsG0780039709.01.T01:CDS
TCAACTCTCTTTGGAGAAGA+TGG 0.362735 7:-67907715 MsG0780039709.01.T01:CDS
CTTTAGTTGTCTTGGTGGCT+GGG 0.366138 7:+67907671 None:intergenic
GCGAAAATCTTTAGTTGTCT+TGG 0.388842 7:+67907663 None:intergenic
TATAACAAAATCTGTTTCCT+TGG 0.413403 7:+67907175 None:intergenic
TCCACATGAAGAGGAACAAC+AGG 0.414042 7:+67907454 None:intergenic
AACCAAAGTTGAACTTGAAA+AGG 0.431037 7:+67907599 None:intergenic
CCCTGTTGTTCCTCTTCATG+TGG 0.432876 7:-67907455 MsG0780039709.01.T01:CDS
CATCAAAATCACGAGGCAAT+AGG 0.433782 7:+67907260 None:intergenic
TGGTGGCTGGGTTCCATAAA+CGG 0.437559 7:+67907683 None:intergenic
CTCTTTGGAGAAGATGGATA+CGG 0.464962 7:-67907709 MsG0780039709.01.T01:CDS
TTCGACCGTCATACTATAGT+TGG 0.465211 7:-67907763 MsG0780039709.01.T01:CDS
AATTAAATGACGCACGGAGT+AGG 0.485225 7:+67907840 None:intergenic
CAAACAGATCAATCCATTGC+AGG 0.487908 7:+67907738 None:intergenic
ATTAAATGACGCACGGAGTA+GGG 0.493494 7:+67907841 None:intergenic
GAGCGGCACTCTTAATTGGT+TGG 0.499742 7:-67907380 MsG0780039709.01.T01:CDS
TTCATCGTCAATGACGGAAT+GGG 0.514194 7:+67907801 None:intergenic
TTGATGTTCTGGATTGTAAC+GGG 0.516060 7:+67907540 None:intergenic
TAATTGTTTCACTTGATTTG+GGG 0.519198 7:-67907303 MsG0780039709.01.T01:CDS
AAAATCTTTAGTTGTCTTGG+TGG 0.521345 7:+67907666 None:intergenic
ATTCATCGTCAATGACGGAA+TGG 0.523407 7:+67907800 None:intergenic
TCGACCGTCATACTATAGTT+GGG 0.531181 7:-67907762 MsG0780039709.01.T01:CDS
CCAGTGTTGTCCACATGAAG+AGG 0.540532 7:+67907445 None:intergenic
ACTATAGTTGGGTCCTGCAA+TGG 0.541065 7:-67907751 MsG0780039709.01.T01:CDS
TGACGGAATGGGTGAACGAA+GGG 0.557582 7:+67907812 None:intergenic
CCTCTTCATGTGGACAACAC+TGG 0.558962 7:-67907445 MsG0780039709.01.T01:CDS
TTCAACTGACACCTATAAAG+TGG 0.563131 7:-67907569 MsG0780039709.01.T01:CDS
GTGGACAACACTGGAAGTGA+AGG 0.597071 7:-67907436 MsG0780039709.01.T01:CDS
TCTTTGGAGAAGATGGATAC+GGG 0.598574 7:-67907708 MsG0780039709.01.T01:CDS
ACAACATTCATCGTCAATGA+CGG 0.601794 7:+67907795 None:intergenic
TCAACAATTGGAGCTTTAGG+TGG 0.614253 7:+67907232 None:intergenic
ATTGATGTTCTGGATTGTAA+CGG 0.619633 7:+67907539 None:intergenic
ATGACGGAATGGGTGAACGA+AGG 0.636054 7:+67907811 None:intergenic
AATTGTGGTGTGTATTTGAG+CGG 0.651541 7:-67907397 MsG0780039709.01.T01:CDS
AACTGACACCTATAAAGTGG+TGG 0.655068 7:-67907566 MsG0780039709.01.T01:CDS
GTTCTCAATTAAATGACGCA+CGG 0.658562 7:+67907834 None:intergenic
TATGATCGTAGTGAGAATTG+TGG 0.693171 7:-67907412 MsG0780039709.01.T01:CDS
AGGACCCAACTATAGTATGA+CGG 0.700381 7:+67907758 None:intergenic
CCACATGAAGAGGAACAACA+GGG 0.708802 7:+67907455 None:intergenic
GGCACCTCATCAAAATCACG+AGG 0.717349 7:+67907253 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! GTAATTGTTTCACTTGATTT+GGG - Chr7:67907741-67907760 MsG0780039709.01.T01:CDS 25.0%
! GTTGATTATGTACTTGATTT+GGG - Chr7:67907168-67907187 MsG0780039709.01.T01:CDS 25.0%
! TATAACAAAATCTGTTTCCT+TGG + Chr7:67907873-67907892 None:intergenic 25.0%
! TCACTTGCACTATAATATTA+AGG - Chr7:67907698-67907717 MsG0780039709.01.T01:CDS 25.0%
! TGGTAGAATAAGAAAAACAA+AGG + Chr7:67907853-67907872 None:intergenic 25.0%
! TGTAATTGTTTCACTTGATT+TGG - Chr7:67907740-67907759 MsG0780039709.01.T01:CDS 25.0%
!! TAATTGTTTCACTTGATTTG+GGG - Chr7:67907742-67907761 MsG0780039709.01.T01:CDS 25.0%
!!! AAACAGATTTTGTTATATGG+CGG - Chr7:67907876-67907895 MsG0780039709.01.T01:CDS 25.0%
!!! AGGAAACAGATTTTGTTATA+TGG - Chr7:67907873-67907892 MsG0780039709.01.T01:CDS 25.0%
!!! TTGTTTTTCTTATTCTACCA+AGG - Chr7:67907853-67907872 MsG0780039709.01.T01:CDS 25.0%
AAAATCTTTAGTTGTCTTGG+TGG + Chr7:67907382-67907401 None:intergenic 30.0%
AACCAAAGTTGAACTTGAAA+AGG + Chr7:67907449-67907468 None:intergenic 30.0%
GGTTGATTATGTACTTGATT+TGG - Chr7:67907167-67907186 MsG0780039709.01.T01:CDS 30.0%
! ATTGATGTTCTGGATTGTAA+CGG + Chr7:67907509-67907528 None:intergenic 30.0%
! TAAGTTTGCGTGATAATGTT+TGG - Chr7:67907550-67907569 MsG0780039709.01.T01:CDS 30.0%
!! TAGAATTTTGACATTGCTTC+TGG + Chr7:67907532-67907551 None:intergenic 30.0%
!!! AACCTTTTCAAGTTCAACTT+TGG - Chr7:67907444-67907463 MsG0780039709.01.T01:CDS 30.0%
!!! TTTCGCTATTTTTATGACTG+TGG - Chr7:67907399-67907418 MsG0780039709.01.T01:CDS 30.0%
AATTGTGGTGTGTATTTGAG+CGG - Chr7:67907648-67907667 MsG0780039709.01.T01:CDS 35.0%
ACAACATTCATCGTCAATGA+CGG + Chr7:67907253-67907272 None:intergenic 35.0%
ACATCAACAATTGGAGCTTT+AGG + Chr7:67907819-67907838 None:intergenic 35.0%
ATCTGTTTGTCAACTCTCTT+TGG - Chr7:67907321-67907340 MsG0780039709.01.T01:CDS 35.0%
GCGAAAATCTTTAGTTGTCT+TGG + Chr7:67907385-67907404 None:intergenic 35.0%
GTTCTCAATTAAATGACGCA+CGG + Chr7:67907214-67907233 None:intergenic 35.0%
TATGATCGTAGTGAGAATTG+TGG - Chr7:67907633-67907652 MsG0780039709.01.T01:CDS 35.0%
TTCAACTGACACCTATAAAG+TGG - Chr7:67907476-67907495 MsG0780039709.01.T01:CDS 35.0%
! AGCTCCAATTGTTGATGTTT+TGG - Chr7:67907821-67907840 MsG0780039709.01.T01:CDS 35.0%
! CTCCAATTGTTGATGTTTTG+GGG - Chr7:67907823-67907842 MsG0780039709.01.T01:CDS 35.0%
! GCTCCAATTGTTGATGTTTT+GGG - Chr7:67907822-67907841 MsG0780039709.01.T01:CDS 35.0%
! TTGATGTTCTGGATTGTAAC+GGG + Chr7:67907508-67907527 None:intergenic 35.0%
!! TCCAATTGTTGATGTTTTGG+GGG - Chr7:67907824-67907843 MsG0780039709.01.T01:CDS 35.0%
AACTGACACCTATAAAGTGG+TGG - Chr7:67907479-67907498 MsG0780039709.01.T01:CDS 40.0%
AATTAAATGACGCACGGAGT+AGG + Chr7:67907208-67907227 None:intergenic 40.0%
AGGACCCAACTATAGTATGA+CGG + Chr7:67907290-67907309 None:intergenic 40.0%
ATTAAATGACGCACGGAGTA+GGG + Chr7:67907207-67907226 None:intergenic 40.0%
ATTTGAGCGGCACTCTTAAT+TGG - Chr7:67907661-67907680 MsG0780039709.01.T01:CDS 40.0%
CAAACAGATCAATCCATTGC+AGG + Chr7:67907310-67907329 None:intergenic 40.0%
CATCAAAATCACGAGGCAAT+AGG + Chr7:67907788-67907807 None:intergenic 40.0%
TCCCCCAAAACATCAACAAT+TGG + Chr7:67907828-67907847 None:intergenic 40.0%
TCGACCGTCATACTATAGTT+GGG - Chr7:67907283-67907302 MsG0780039709.01.T01:CDS 40.0%
TTCGACCGTCATACTATAGT+TGG - Chr7:67907282-67907301 MsG0780039709.01.T01:CDS 40.0%
TTGCTTCTGGATTGATGTTC+TGG + Chr7:67907519-67907538 None:intergenic 40.0%
! ATTCATCGTCAATGACGGAA+TGG + Chr7:67907248-67907267 None:intergenic 40.0%
! CTCTTTGGAGAAGATGGATA+CGG - Chr7:67907336-67907355 MsG0780039709.01.T01:CDS 40.0%
! TCAACTCTCTTTGGAGAAGA+TGG - Chr7:67907330-67907349 MsG0780039709.01.T01:CDS 40.0%
! TCTTTGGAGAAGATGGATAC+GGG - Chr7:67907337-67907356 MsG0780039709.01.T01:CDS 40.0%
! TTCATCGTCAATGACGGAAT+GGG + Chr7:67907247-67907266 None:intergenic 40.0%
!! ATTGCCTCGTGATTTTGATG+AGG - Chr7:67907788-67907807 MsG0780039709.01.T01:CDS 40.0%
!! TCAACAATTGGAGCTTTAGG+TGG + Chr7:67907816-67907835 None:intergenic 40.0%
ACTATAGTTGGGTCCTGCAA+TGG - Chr7:67907294-67907313 MsG0780039709.01.T01:CDS 45.0%
ATGGATACGGGTTCCGTTTA+TGG - Chr7:67907349-67907368 MsG0780039709.01.T01:CDS 45.0%
CCACATGAAGAGGAACAACA+GGG + Chr7:67907593-67907612 None:intergenic 45.0%
TCCACATGAAGAGGAACAAC+AGG + Chr7:67907594-67907613 None:intergenic 45.0%
! CTTTAGTTGTCTTGGTGGCT+GGG + Chr7:67907377-67907396 None:intergenic 45.0%
! TCTTTAGTTGTCTTGGTGGC+TGG + Chr7:67907378-67907397 None:intergenic 45.0%
ACGGGACACCACCACTTTAT+AGG + Chr7:67907490-67907509 None:intergenic 50.0%
CCAGTGTTGTCCACATGAAG+AGG + Chr7:67907603-67907622 None:intergenic 50.0%
CCCTGTTGTTCCTCTTCATG+TGG - Chr7:67907590-67907609 MsG0780039709.01.T01:CDS 50.0%
CCTCTTCATGTGGACAACAC+TGG - Chr7:67907600-67907619 MsG0780039709.01.T01:CDS 50.0%
GAGCGGCACTCTTAATTGGT+TGG - Chr7:67907665-67907684 MsG0780039709.01.T01:CDS 50.0%
GGCACCTCATCAAAATCACG+AGG + Chr7:67907795-67907814 None:intergenic 50.0%
GTGGACAACACTGGAAGTGA+AGG - Chr7:67907609-67907628 MsG0780039709.01.T01:CDS 50.0%
TGACGGAATGGGTGAACGAA+GGG + Chr7:67907236-67907255 None:intergenic 50.0%
TGGTGGCTGGGTTCCATAAA+CGG + Chr7:67907365-67907384 None:intergenic 50.0%
! ATGACGGAATGGGTGAACGA+AGG + Chr7:67907237-67907256 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
Chr7 gene 67907165 67907902 67907165 ID=MsG0780039709.01;Name=MsG0780039709.01
Chr7 mRNA 67907165 67907902 67907165 ID=MsG0780039709.01.T01;Parent=MsG0780039709.01;Name=MsG0780039709.01.T01;_AED=0.39;_eAED=0.40;_QI=0|-1|0|1|-1|1|1|0|245
Chr7 exon 67907165 67907902 67907165 ID=MsG0780039709.01.T01:exon:11651;Parent=MsG0780039709.01.T01
Chr7 CDS 67907165 67907902 67907165 ID=MsG0780039709.01.T01:cds;Parent=MsG0780039709.01.T01
Gene Sequence

>MsG0780039709.01.T01

ATGGTTGATTATGTACTTGATTTGGGTTACAGTGTCATTCCCTACTCCGTGCGTCATTTAATTGAGAACCCTTCGTTCACCCATTCCGTCATTGACGATGAATGTTGTTTTACATGCTTCGACCGTCATACTATAGTTGGGTCCTGCAATGGATTGATCTGTTTGTCAACTCTCTTTGGAGAAGATGGATACGGGTTCCGTTTATGGAACCCAGCCACCAAGACAACTAAAGATTTTCGCTATTTTTATGACTGTGGCAGCAGCTCTCAACGCAATACTAACCTTTTCAAGTTCAACTTTGGTTGTGACATTTCAACTGACACCTATAAAGTGGTGGTGTCCCGTTACAATCCAGAACATCAATCCAGAAGCAATGTCAAAATTCTAAGTTTGCGTGATAATGTTTGGAGAGATATTGAAAGTTTCCCTGTTGTTCCTCTTCATGTGGACAACACTGGAAGTGAAGGTTATGATCGTAGTGAGAATTGTGGTGTGTATTTGAGCGGCACTCTTAATTGGTTGGCTATTCACAATCACTTGCACTATAATATTAAGGATATTACAGTTAAGCAGTTTGTAATTGTTTCACTTGATTTGGGGACTGAGACGTACAATAAATACCTATTGCCTCGTGATTTTGATGAGGTGCCACCTAAAGCTCCAATTGTTGATGTTTTGGGGGATGCCTTTGTTTTTCTTATTCTACCAAGGAAACAGATTTTGTTATATGGCGGATGA
Protein sequence

>MsG0780039709.01.T01

MVDYVLDLGYSVIPYSVRHLIENPSFTHSVIDDECCFTCFDRHTIVGSCNGLICLSTLFGEDGYGFRLWNPATKTTKDFRYFYDCGSSSQRNTNLFKFNFGCDISTDTYKVVVSRYNPEHQSRSNVKILSLRDNVWRDIESFPVVPLHVDNTGSEGYDRSENCGVYLSGTLNWLAIHNHLHYNIKDITVKQFVIVSLDLGTETYNKYLLPRDFDEVPPKAPIVDVLGDAFVFLILPRKQILLYGG*