AlfalfaGEDB | MsG0680034387.01

M. sativa cultivar ZhongmuNo.1 / MsG0680034387.01

KEGG: -
GO: GO:0004866 GO:0005515
PFAM: PF00197 PF12937

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680034387.01.T01	XP_024642286.1	83.214	280	39	5	127	405	8	280	6.28E-158	461

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680034387.01.T01	O82711	47.101	138	66	5	399	533	75	208	7.47E-26	108

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680034387.01.T01	A0A396HFZ8	83.214	280	39	5	127	405	8	280	3.00e-158	461

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
MsG0080048101.01	MsG0680034387.01	PPI
MsG0080048487.01	MsG0680034387.01	PPI
MsG0080048487.01	MsG0680034387.01	PPI
MsG0180000330.01	MsG0680034387.01	PPI
MsG0180000330.01	MsG0680034387.01	PPI
MsG0180000837.01	MsG0680034387.01	PPI
MsG0180000838.01	MsG0680034387.01	PPI
MsG0180001056.01	MsG0680034387.01	PPI
MsG0180001056.01	MsG0680034387.01	PPI
MsG0180001514.01	MsG0680034387.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680034387.01.T01	MTR_6g065510	84.791	263	36	3	144	405	72	331	4.62e-158	454
MsG0680034387.01.T01	MTR_6g065510	87.143	70	9	0	1	70	1	70	1.01e-30	122
MsG0680034387.01.T01	MTR_5g069760	58.673	392	130	11	21	405	4	370	1.97e-141	413
MsG0680034387.01.T01	MTR_4g129190	55.696	395	153	10	23	405	6	390	9.03e-141	412
MsG0680034387.01.T01	MTR_7g055977	55.754	391	161	5	17	405	1	381	4.86e-137	402
MsG0680034387.01.T01	MTR_5g069930	56.266	391	140	11	21	401	4	373	8.87e-137	401
MsG0680034387.01.T01	MTR_7g055953	75.686	255	40	5	168	405	66	315	3.23e-126	372
MsG0680034387.01.T01	MTR_2g043410	44.860	428	192	10	1	400	1	412	9.29e-108	328
MsG0680034387.01.T01	MTR_2g043400	45.409	403	179	10	25	400	25	413	2.58e-104	320
MsG0680034387.01.T01	MTR_7g056290	46.552	406	174	13	25	400	24	416	1.91e-103	317

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680034387.01.T01	AT3G23880	26.316	380	220	14	23	390	14	345	1.46e-22	99.8
MsG0680034387.01.T01	AT3G06240	24.682	393	211	13	15	364	28	378	2.41e-20	94.0
MsG0680034387.01.T01	AT4G12560	26.524	328	192	9	23	329	4	303	2.49e-20	94.0
MsG0680034387.01.T01	AT4G12560	26.524	328	192	9	23	329	4	303	2.49e-20	94.0
MsG0680034387.01.T01	AT4G12560	26.524	328	192	9	23	329	4	303	2.49e-20	94.0
MsG0680034387.01.T01	AT1G33530	25.836	329	191	12	5	323	79	364	4.59e-14	74.7
MsG0680034387.01.T01	AT3G49980	24.484	339	199	13	23	343	5	304	3.72e-12	68.6
MsG0680034387.01.T01	AT3G49980	24.484	339	199	13	23	343	6	305	3.94e-12	68.6
MsG0680034387.01.T01	AT3G16210	25.294	340	177	18	22	343	4	284	5.46e-11	64.7

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Find 130 sgRNAs with CRISPR-Local

Find 395 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATTGTTGGTTCCTGCAA+TGG	0.439896	6:-86744526	MsG0680034387.01.T01:CDS
AAAGGTAACGATATCCTTAA+TGG	0.233486	6:-86739186	MsG0680034387.01.T01:CDS
AACGAAGGAGTGTATTTGAG+TGG	0.646395	6:-86744205	MsG0680034387.01.T01:CDS
AACTACCGATTGAGTTACAA+GGG	0.633395	6:-86744556	MsG0680034387.01.T01:CDS
AAGCAATTTCAGACCCCTCG+GGG	0.662586	6:-86744070	MsG0680034387.01.T01:CDS
AAGGGTTATACGAAAATTGT+TGG	0.431055	6:-86744538	MsG0680034387.01.T01:CDS
AAGGTGTAGTTTGACGAATA+TGG	0.353118	6:+86744717	None:intergenic
AAGTTTAGCAGGCTAACTAT+AGG	0.382582	6:-86739354	MsG0680034387.01.T01:CDS
AATACCATGGAAATTCGTCA+AGG	0.670434	6:-86743720	MsG0680034387.01.T01:CDS
AATAGAGTAGAGCAGGCTAG+TGG	0.656795	6:-86743749	MsG0680034387.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAGGTAACGATATCCTTAA+TGG	-	Chr6:86744693-86744712	MsG0680034387.01.T01:CDS	30.0%
AACACAATATGACATGTCAT+CGG	-	Chr6:86744429-86744448	MsG0680034387.01.T01:CDS	30.0%
AACACATCTGAAGATTTATG+TGG	+	Chr6:86744213-86744232	None:intergenic	30.0%
AACGAAGAAATCATGCAAAT+CGG	+	Chr6:86744243-86744262	None:intergenic	30.0%
AAGGGTTATACGAAAATTGT+TGG	-	Chr6:86739341-86739360	MsG0680034387.01.T01:CDS	30.0%
ACCCTAAAATTGCTATGTTT+CGG	-	Chr6:86744278-86744297	MsG0680034387.01.T01:CDS	30.0%
ACCTATATTGATGCATGATT+AGG	+	Chr6:86741196-86741215	None:intergenic	30.0%
AGAATGTTCAAGTAGATGAT+AGG	-	Chr6:86739987-86740006	MsG0680034387.01.T01:intron	30.0%
ATATCAACACTCTTGAAGTA+GGG	+	Chr6:86741672-86741691	None:intergenic	30.0%
ATCAATCAACAAAACTTTGG+GGG	+	Chr6:86740801-86740820	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr6	gene	86738993	86744908	86738993	ID=MsG0680034387.01;Name=MsG0680034387.01
Chr6	mRNA	86738993	86744908	86738993	ID=MsG0680034387.01.T01;Parent=MsG0680034387.01;Name=MsG0680034387.01.T01;_AED=0.03;_eAED=0.03;_QI=0\|0\|0\|1\|1\|1\|2\|0\|538
Chr6	exon	86743708	86744908	86743708	ID=MsG0680034387.01.T01:exon:7368;Parent=MsG0680034387.01.T01
Chr6	exon	86738993	86739408	86738993	ID=MsG0680034387.01.T01:exon:7367;Parent=MsG0680034387.01.T01
Chr6	CDS	86743708	86744908	86743708	ID=MsG0680034387.01.T01:cds;Parent=MsG0680034387.01.T01
Chr6	CDS	86738993	86739408	86738993	ID=MsG0680034387.01.T01:cds;Parent=MsG0680034387.01.T01

Gene Sequence

>MsG0680034387.01.T01

ATGAATCCCCCTCATGCCAGGTCGTCGAAATCCATCGTCATTCAAGCCATGTCGCAGCATGTATACATCCCGGATGAACTCATCGCAGAAGTCCTATCATTCCTTCCGGTGAAATCTCTTCTGCGATTGAAGTGGGTGAGCAAGTCATGGATGAATCTCATCTCCGATCCCATATTCGTCAAACTACACCTTCATCGATCCTCAAGAAAGAAGCACATAGCAGTAATCGAACCAGTAATCGAACATGGAATAGTATGTCGAAATAATGTACTAACCTTTCCCCTGAATCATTTGTTGGAGAACCCATCCATCATCATTGCCCAAAGCCCTAACTACCGATTGAGTTACAAGGGTTATACGAAAATTGTTGGTTCCTGCAATGGATTGGTCTGTTTGCTTGGAGAATCTGACAGTGAGAATTTTGCACGTACTTGGTTTCGTATCTGGAATCCAGCCACTAGGATAATATCTGAAAAACTAGGATCTTTTAAAGAGTGTCACAACGGTTTATATTGCAATTGCAGGCGTAGATTCACATTTGGGTATGATAATTCAACTGGAACTTATAAGGTGGTGCTCTTGTGTTCTAGTAAACTGAAAGTTTTCAGTTTCAGTGATAATATTTGGAGAAAAATTTCAAGTTTTCCTTTTGACCATGCTGTTACTCTTACCTCTGACAGGAACGAAGGAGTGTATTTGAGTGGCACTGTTAATTGGTTTGCCATTCGAAACAAGGCCATTTATAATTCTTATAATAAGGATATTACCGTTGAGCAATTCATGATTATCTCCTTTGATCTTGGTACCGAGACATACAAGCAATTTCAGACCCCTCGGGGTGTTGATGAAGTGCCAGATATTGAACCGACTATTGCAGTGTTGATGGACTGCCTTTGTTTTTCTCACCATATCAAGAGAACTCATTTTGTTATACGGAAGATGTCAGAATTTGGAGTCGAACAATCTTGGACTCAATTCATCAAAATCAGTTTTCAGAATGTTCAAGTAGATGATAGGTTTAGTGACTGGATTCATTATCAGTCGGTTATGTTTCCATTGTGTCTTTCTGAGAATGGTGACATACTAATACTGGTCACTTATCAAAGTGAAAAAGCAATTCTCTATCATTTGAAAGATAATAGAGTAGAGCAGGCTAGTGGTAATGAAATACCATGGAAATTCGTCAAGGATTATGTTGAAGATTATTCTGAAGTTGTTAATGGTCTGCCTATAAAGTTTAGCAGGCTAACTATAGGGATTGATATAATCTCTACAGACACACCACTTAATATTTCATTTACAAGAAAGCCCGAGTGTGCTGAATGCTCCAAGTGGGTGTTGGTTGAAGATTTTAACAAACTATTTGGACCATGGGTGAGTATTGGTGGTACTGAAGACAATAAAGGTAACGATATCCTTAATGGTTTCTTTGAAATTCAGAAACATGGTTTTGGATACACGTTTTTGTTTTGTCCAACCATCACTGCCCCACCTGGTGCTTGTTATGATATTGGGAGGCGTAATGACGTGACTGGAAGACGTCTGATCCTGGCTGAAAATGATCCCTTTGAAGTTGTATTTGTTGATGCCTCTGAAAATGGAAGATCTTTAGTTTGA

Protein sequence

>MsG0680034387.01.T01

MNPPHARSSKSIVIQAMSQHVYIPDELIAEVLSFLPVKSLLRLKWVSKSWMNLISDPIFVKLHLHRSSRKKHIAVIEPVIEHGIVCRNNVLTFPLNHLLENPSIIIAQSPNYRLSYKGYTKIVGSCNGLVCLLGESDSENFARTWFRIWNPATRIISEKLGSFKECHNGLYCNCRRRFTFGYDNSTGTYKVVLLCSSKLKVFSFSDNIWRKISSFPFDHAVTLTSDRNEGVYLSGTVNWFAIRNKAIYNSYNKDITVEQFMIISFDLGTETYKQFQTPRGVDEVPDIEPTIAVLMDCLCFSHHIKRTHFVIRKMSEFGVEQSWTQFIKISFQNVQVDDRFSDWIHYQSVMFPLCLSENGDILILVTYQSEKAILYHLKDNRVEQASGNEIPWKFVKDYVEDYSEVVNGLPIKFSRLTIGIDIISTDTPLNISFTRKPECAECSKWVLVEDFNKLFGPWVSIGGTEDNKGNDILNGFFEIQKHGFGYTFLFCPTITAPPGACYDIGRRNDVTGRRLILAENDPFEVVFVDASENGRSLV*