AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780038559.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780038559.01.T01 MTR_7g056760 85.648 216 27 1 65 276 176 391 1.15e-133 384
MsG0780038559.01.T01 MTR_7g056760 85.484 62 9 0 1 62 41 102 1.62e-27 110
MsG0780038559.01.T01 MTR_7g056797 73.684 209 53 2 68 274 179 387 1.40e-103 307
MsG0780038559.01.T01 MTR_7g056797 88.710 62 7 0 1 62 41 102 1.12e-29 115
MsG0780038559.01.T01 MTR_7g022670 47.667 300 130 8 1 276 41 337 9.42e-84 255
MsG0780038559.01.T01 MTR_7g022600 38.814 371 129 10 1 276 41 408 1.07e-70 224
MsG0780038559.01.T01 MTR_7g062100 39.655 348 131 12 1 275 42 383 4.94e-62 201
MsG0780038559.01.T01 MTR_0128s0070 39.031 351 130 11 1 275 40 382 5.15e-54 180
MsG0780038559.01.T01 MTR_4g011660 41.949 236 105 6 65 275 175 403 1.93e-49 169
MsG0780038559.01.T01 MTR_4g011660 53.226 62 28 1 1 61 37 98 5.23e-11 62.8
MsG0780038559.01.T01 MTR_7g055830 35.826 321 149 11 2 275 32 342 1.98e-47 162
MsG0780038559.01.T01 MTR_7g056503 41.667 216 123 3 63 276 170 384 4.92e-47 162
MsG0780038559.01.T01 MTR_0128s0010 42.523 214 113 6 68 275 174 383 7.05e-47 161
MsG0780038559.01.T01 MTR_0128s0020 45.794 214 103 5 65 275 176 379 1.64e-46 160
MsG0780038559.01.T01 MTR_0128s0020 56.716 67 20 3 1 62 36 98 6.59e-12 65.5
MsG0780038559.01.T01 MTR_7g061940 44.843 223 103 8 46 260 56 266 1.80e-46 157
MsG0780038559.01.T01 MTR_5g096710 43.721 215 110 6 68 276 176 385 2.88e-46 160
MsG0780038559.01.T01 MTR_5g096710 57.576 66 25 2 1 66 41 103 3.82e-12 66.2
MsG0780038559.01.T01 MTR_7g058630 53.896 154 58 5 131 275 16 165 6.07e-46 152
MsG0780038559.01.T01 MTR_1g071010 40.625 224 110 6 65 276 161 373 6.55e-46 159
MsG0780038559.01.T01 MTR_7g057270 35.000 260 145 6 30 275 150 399 7.22e-44 154
MsG0780038559.01.T01 MTR_2g006360 35.738 305 137 13 1 271 64 343 4.56e-43 150
MsG0780038559.01.T01 MTR_7g445890 32.344 337 164 10 1 275 34 368 2.97e-41 146
MsG0780038559.01.T01 MTR_7g113500 32.819 259 151 5 30 275 119 367 1.31e-40 144
MsG0780038559.01.T01 MTR_7g078040 40.265 226 109 6 67 271 182 402 1.90e-40 145
MsG0780038559.01.T01 MTR_3g013470 31.268 355 160 10 2 276 14 364 4.12e-40 143
MsG0780038559.01.T01 MTR_4g013180 32.000 350 156 11 1 272 29 374 1.51e-39 144
MsG0780038559.01.T01 MTR_7g056200 33.033 333 159 14 1 275 41 367 2.57e-39 141
MsG0780038559.01.T01 MTR_6g065510 34.899 298 146 12 1 273 41 315 5.59e-39 139
MsG0780038559.01.T01 MTR_7g066850 39.726 219 107 7 67 266 168 380 9.43e-39 141
MsG0780038559.01.T01 MTR_7g077990 30.088 339 166 9 1 271 35 370 2.29e-38 139
MsG0780038559.01.T01 MTR_7g055870 39.056 233 108 8 67 275 188 410 2.62e-38 139
MsG0780038559.01.T01 MTR_2g043410 40.260 231 107 7 67 275 181 402 2.76e-38 139
MsG0780038559.01.T01 MTR_7g055930 39.056 233 108 8 67 275 188 410 4.58e-38 139
MsG0780038559.01.T01 MTR_7g078860 38.627 233 119 8 60 276 160 384 9.24e-38 137
MsG0780038559.01.T01 MTR_5g069760 40.291 206 107 6 67 268 156 349 3.31e-37 135
MsG0780038559.01.T01 MTR_8g066440 38.356 219 114 6 67 274 178 386 3.43e-37 136
MsG0780038559.01.T01 MTR_7g066840 40.741 216 100 9 67 266 170 373 4.71e-37 135
MsG0780038559.01.T01 MTR_7g091630 37.712 236 117 7 67 275 184 416 7.54e-37 135
MsG0780038559.01.T01 MTR_2g010950 35.371 229 132 5 60 275 165 390 1.06e-36 135
MsG0780038559.01.T01 MTR_2g010920 35.874 223 125 5 68 275 191 410 1.21e-36 135
MsG0780038559.01.T01 MTR_6g055210 39.004 241 110 9 67 276 172 406 1.27e-36 135
MsG0780038559.01.T01 MTR_6g017305 33.916 286 146 13 14 275 1 267 2.23e-36 131
MsG0780038559.01.T01 MTR_7g079160 39.269 219 113 8 67 271 191 403 4.28e-36 134
MsG0780038559.01.T01 MTR_5g056020 37.238 239 117 7 67 276 173 407 8.86e-36 134
MsG0780038559.01.T01 MTR_4g013185 38.393 224 114 6 67 271 178 396 1.09e-35 132
MsG0780038559.01.T01 MTR_7g078840 38.288 222 111 7 67 268 184 399 1.79e-35 132
MsG0780038559.01.T01 MTR_7g034310 37.736 212 100 6 67 268 20 209 1.84e-35 127
MsG0780038559.01.T01 MTR_7g066100 37.736 212 119 4 67 271 169 374 4.63e-35 130
MsG0780038559.01.T01 MTR_2g047892 38.182 220 108 9 67 272 174 379 7.00e-35 129
MsG0780038559.01.T01 MTR_5g069930 37.302 252 118 11 30 268 132 356 8.21e-35 129
MsG0780038559.01.T01 MTR_7g055953 38.208 212 107 7 67 266 93 292 9.94e-35 127
MsG0780038559.01.T01 MTR_3g080370 35.336 283 127 11 30 276 142 404 1.06e-34 130
MsG0780038559.01.T01 MTR_7g089830 47.020 151 51 6 124 260 6 141 1.31e-34 122
MsG0780038559.01.T01 MTR_6g088385 37.391 230 115 7 66 275 84 304 1.52e-34 127
MsG0780038559.01.T01 MTR_7g055977 40.654 214 98 8 67 264 156 356 2.81e-34 127
MsG0780038559.01.T01 MTR_5g068040 36.842 228 110 9 67 275 154 366 4.67e-34 127
MsG0780038559.01.T01 MTR_4g129190 30.110 362 157 12 1 275 24 376 6.33e-34 127
MsG0780038559.01.T01 MTR_0242s0060 35.745 235 120 6 57 270 164 388 7.24e-34 127
MsG0780038559.01.T01 MTR_0242s0040 35.745 235 120 6 57 270 164 388 7.24e-34 127
MsG0780038559.01.T01 MTR_4g030830 36.123 227 119 5 67 274 158 377 1.12e-33 126
MsG0780038559.01.T01 MTR_7g056003 34.686 271 130 9 30 275 145 393 1.16e-33 127
MsG0780038559.01.T01 MTR_4g013175 36.000 225 120 6 67 272 178 397 1.22e-33 127
MsG0780038559.01.T01 MTR_7g056173 36.957 230 117 6 67 275 30 252 2.19e-33 124
MsG0780038559.01.T01 MTR_7g062060 37.500 216 119 6 67 275 184 390 2.43e-33 125
MsG0780038559.01.T01 MTR_7g070830 36.937 222 123 8 67 275 188 405 3.31e-33 125
MsG0780038559.01.T01 MTR_4g024890 35.841 226 132 5 60 275 148 370 5.72e-33 124
MsG0780038559.01.T01 MTR_4g008790 29.396 364 158 10 2 271 58 416 6.91e-33 125
MsG0780038559.01.T01 MTR_2g017720 32.271 251 144 7 34 274 50 284 9.74e-33 124
MsG0780038559.01.T01 MTR_7g060960 31.804 327 143 12 1 273 50 350 1.23e-32 123
MsG0780038559.01.T01 MTR_7g078900 37.557 221 114 6 67 271 180 392 1.55e-32 124
MsG0780038559.01.T01 MTR_7g056290 36.052 233 114 8 67 275 185 406 1.67e-32 124
MsG0780038559.01.T01 MTR_7g056710 38.389 211 101 8 66 264 166 359 1.85e-32 123
MsG0780038559.01.T01 MTR_2g043400 37.500 232 113 7 67 275 181 403 2.29e-32 123
MsG0780038559.01.T01 MTR_5g058620 35.321 218 127 6 67 271 36 252 2.57e-32 120
MsG0780038559.01.T01 MTR_1g058870 35.321 218 127 6 67 271 36 252 2.57e-32 120
MsG0780038559.01.T01 MTR_0097s0030 36.530 219 111 9 66 275 164 363 3.25e-32 122
MsG0780038559.01.T01 MTR_2g047240 36.530 219 111 9 66 275 164 363 3.25e-32 122
MsG0780038559.01.T01 MTR_7g060940 34.962 266 123 9 30 276 145 379 3.53e-32 122
MsG0780038559.01.T01 MTR_7g077970 36.726 226 115 6 67 271 173 391 3.72e-32 123
MsG0780038559.01.T01 MTR_2g027820 33.446 296 159 11 1 273 1 281 3.97e-32 120
MsG0780038559.01.T01 MTR_1g015740 31.023 303 146 10 1 271 26 297 3.98e-32 121
MsG0780038559.01.T01 MTR_6g016285 33.775 302 157 9 8 275 111 403 4.87e-32 123
MsG0780038559.01.T01 MTR_0707s0020 35.683 227 119 7 67 275 212 429 5.71e-32 122
MsG0780038559.01.T01 MTR_7g066000 34.685 222 133 6 61 275 171 387 6.33e-32 122
MsG0780038559.01.T01 MTR_5g043160 35.747 221 119 10 67 268 220 436 7.19e-32 122
MsG0780038559.01.T01 MTR_7g091610 37.379 206 113 6 83 275 30 232 7.92e-32 118
MsG0780038559.01.T01 MTR_7g028660 33.790 219 115 6 62 266 154 356 1.03e-31 121
MsG0780038559.01.T01 MTR_7g066030 36.576 257 134 9 30 272 145 386 1.39e-31 121
MsG0780038559.01.T01 MTR_3g437520 36.818 220 113 5 67 266 188 401 1.91e-31 121
MsG0780038559.01.T01 MTR_7g070880 36.036 222 125 8 67 275 188 405 2.08e-31 120
MsG0780038559.01.T01 MTR_1g088120 36.607 224 119 8 67 272 164 382 3.09e-31 120
MsG0780038559.01.T01 MTR_6g017295 31.773 299 152 12 14 276 51 333 3.88e-31 121
MsG0780038559.01.T01 MTR_7g056390 34.764 233 117 8 67 275 181 402 3.91e-31 120
MsG0780038559.01.T01 MTR_2g047200 35.573 253 120 12 30 266 146 371 4.36e-31 119
MsG0780038559.01.T01 MTR_4g031020 34.649 228 124 7 67 275 192 413 4.36e-31 120
MsG0780038559.01.T01 MTR_2g010935 33.491 212 117 5 69 275 43 235 4.39e-31 116
MsG0780038559.01.T01 MTR_3g464500 34.081 223 128 5 67 275 171 388 4.76e-31 119
MsG0780038559.01.T01 MTR_7g050480 35.945 217 113 7 67 273 172 372 5.65e-31 119
MsG0780038559.01.T01 MTR_7g055987 40.000 170 81 4 63 226 164 318 6.82e-31 118
MsG0780038559.01.T01 MTR_7g050520 34.177 237 123 8 54 273 152 372 9.54e-31 118
MsG0780038559.01.T01 MTR_7g061010 35.321 218 111 6 67 273 172 370 9.65e-31 118
MsG0780038559.01.T01 MTR_4g030980 34.649 228 124 7 67 275 192 413 1.10e-30 119
MsG0780038559.01.T01 MTR_6g081280 33.621 232 127 5 56 275 10 226 1.26e-30 115
MsG0780038559.01.T01 MTR_6g017280 34.101 217 128 4 67 274 162 372 1.45e-30 118
MsG0780038559.01.T01 MTR_7g061060 34.419 215 118 5 67 271 168 369 1.52e-30 118
MsG0780038559.01.T01 MTR_3g464470 34.513 226 123 6 67 275 148 365 2.29e-30 117
MsG0780038559.01.T01 MTR_7g091640 31.126 302 124 10 1 224 53 348 2.71e-30 117
MsG0780038559.01.T01 MTR_0464s0020 36.323 223 113 6 67 266 188 404 4.99e-30 117
MsG0780038559.01.T01 MTR_2g461450 29.969 327 156 10 1 266 36 350 5.59e-30 115
MsG0780038559.01.T01 MTR_2g047895 36.161 224 105 8 67 275 96 296 6.47e-30 115
MsG0780038559.01.T01 MTR_7g077910 39.648 227 109 9 67 271 90 310 6.63e-30 115
MsG0780038559.01.T01 MTR_4g030090 35.417 240 135 10 53 275 149 385 9.47e-30 116
MsG0780038559.01.T01 MTR_4g034860 35.268 224 116 7 63 273 187 394 1.12e-29 115
MsG0780038559.01.T01 MTR_3g072470 35.714 224 109 8 67 266 181 393 1.12e-29 116
MsG0780038559.01.T01 MTR_2g010900 34.562 217 117 7 68 271 138 342 2.31e-29 114
MsG0780038559.01.T01 MTR_2g010900 44.286 70 33 2 1 70 1 64 9.55e-11 62.0
MsG0780038559.01.T01 MTR_1g442850 36.916 214 107 7 67 268 160 357 2.80e-29 114
MsG0780038559.01.T01 MTR_7g078880 32.168 286 142 10 1 268 27 278 3.15e-29 114
MsG0780038559.01.T01 MTR_7g056737 37.209 215 112 7 61 266 166 366 4.11e-29 114
MsG0780038559.01.T01 MTR_7g052670 33.061 245 125 9 60 275 176 410 4.49e-29 115
MsG0780038559.01.T01 MTR_2g047878 36.190 210 104 6 67 275 169 349 9.53e-29 112
MsG0780038559.01.T01 MTR_7g009265 32.599 227 138 4 63 275 84 309 1.41e-28 111
MsG0780038559.01.T01 MTR_0649s0020 33.080 263 140 10 30 275 130 373 2.57e-28 112
MsG0780038559.01.T01 MTR_6g086920 34.167 240 119 8 67 275 224 455 2.78e-28 113
MsG0780038559.01.T01 MTR_4g019990 35.714 224 122 7 67 275 158 374 5.25e-28 111
MsG0780038559.01.T01 MTR_7g061050 34.314 204 121 6 67 264 165 361 8.16e-28 110
MsG0780038559.01.T01 MTR_1g029650 34.247 219 118 9 67 271 168 374 1.55e-27 110
MsG0780038559.01.T01 MTR_7g058660 54.455 101 36 2 184 275 101 200 1.83e-27 106
MsG0780038559.01.T01 MTR_7g056260 42.604 169 77 4 61 219 158 316 2.05e-27 108
MsG0780038559.01.T01 MTR_7g056717 35.294 221 118 8 61 272 166 370 4.70e-27 108
MsG0780038559.01.T01 MTR_1g015730 34.419 215 118 7 67 271 164 365 6.55e-27 108
MsG0780038559.01.T01 MTR_1g112010 31.004 229 134 6 47 275 106 310 1.72e-26 105
MsG0780038559.01.T01 MTR_7g061090 32.510 243 134 8 30 262 159 381 1.75e-26 107
MsG0780038559.01.T01 MTR_7g077070 34.906 212 122 6 67 264 183 392 2.81e-26 107
MsG0780038559.01.T01 MTR_4g006350 29.730 222 141 5 67 275 641 860 4.23e-26 107
MsG0780038559.01.T01 MTR_4g033495 34.956 226 118 10 61 273 180 389 5.31e-26 106
MsG0780038559.01.T01 MTR_4g129160 36.893 206 112 9 55 247 160 360 6.35e-26 105
MsG0780038559.01.T01 MTR_7g078960 35.885 209 110 7 67 264 55 250 1.06e-25 103
MsG0780038559.01.T01 MTR_8g030630 36.111 180 93 6 90 266 279 439 1.21e-25 105
MsG0780038559.01.T01 MTR_8g030630 35.795 176 100 4 112 275 71 245 3.62e-22 95.5
MsG0780038559.01.T01 MTR_7g052150 32.751 229 118 9 67 276 174 385 1.36e-25 104
MsG0780038559.01.T01 MTR_7g006360 30.662 287 112 9 1 262 27 251 5.87e-25 101
MsG0780038559.01.T01 MTR_7g062070 45.614 114 54 4 156 263 75 186 9.16e-24 95.9
MsG0780038559.01.T01 MTR_6g092560 32.609 230 122 7 67 275 164 381 1.50e-23 99.8
MsG0780038559.01.T01 MTR_4g030790 32.374 278 138 13 1 242 37 300 3.77e-23 97.1
MsG0780038559.01.T01 MTR_2g060555 30.147 272 149 10 6 275 41 273 4.63e-23 95.9
MsG0780038559.01.T01 MTR_7g056570 31.754 211 126 6 61 264 162 361 4.97e-23 97.8
MsG0780038559.01.T01 MTR_4g030760 32.558 215 122 7 67 274 216 414 1.79e-22 96.3
MsG0780038559.01.T01 MTR_2g437110 28.679 265 156 9 19 275 139 378 2.81e-22 96.3
MsG0780038559.01.T01 MTR_0267s0050 29.644 253 155 7 30 273 130 368 3.43e-22 95.1
MsG0780038559.01.T01 MTR_4g020000 32.710 214 125 5 67 268 116 322 3.48e-22 94.7
MsG0780038559.01.T01 MTR_6g042600 30.855 269 151 11 1 261 54 295 4.67e-22 95.1
MsG0780038559.01.T01 MTR_3g055000 37.903 124 64 3 153 275 1 112 5.97e-22 89.0
MsG0780038559.01.T01 MTR_7g037670 28.660 321 129 11 1 270 41 312 2.15e-21 92.4
MsG0780038559.01.T01 MTR_7g056820 30.876 217 133 6 67 275 155 362 2.52e-21 92.8
MsG0780038559.01.T01 MTR_7g070900 30.159 252 107 8 4 244 202 395 5.11e-21 92.4
MsG0780038559.01.T01 MTR_7g056793 29.907 214 94 7 67 273 51 215 1.25e-20 88.2
MsG0780038559.01.T01 MTR_4g030110 31.799 239 118 6 57 275 169 382 1.80e-20 90.1
MsG0780038559.01.T01 MTR_3g013950 28.175 252 162 6 30 273 130 370 4.63e-20 89.4
MsG0780038559.01.T01 MTR_7g066010 31.100 209 97 6 67 268 182 350 7.69e-20 88.2
MsG0780038559.01.T01 MTR_4g037465 30.000 210 129 5 67 266 170 371 1.61e-19 87.8
MsG0780038559.01.T01 MTR_7g066060 30.531 226 131 7 67 276 181 396 7.06e-19 86.3
MsG0780038559.01.T01 MTR_7g445750 35.443 158 96 2 55 207 173 329 8.75e-19 85.1
MsG0780038559.01.T01 MTR_5g069950 34.146 164 78 6 67 226 66 203 9.99e-19 82.8
MsG0780038559.01.T01 MTR_7g070937 40.833 120 56 5 66 172 14 131 3.60e-18 80.1
MsG0780038559.01.T01 MTR_7g070440 30.508 236 131 10 57 275 134 353 3.60e-18 83.6
MsG0780038559.01.T01 MTR_4g024910 29.123 285 158 11 1 275 1 251 1.85e-17 81.3
MsG0780038559.01.T01 MTR_3g055070 37.815 119 60 4 153 270 1 106 2.74e-17 77.4
MsG0780038559.01.T01 MTR_7g056170 28.926 242 120 7 52 275 238 445 2.44e-16 79.0
MsG0780038559.01.T01 MTR_8g433030 29.075 227 143 6 4 222 61 277 2.58e-16 78.2
MsG0780038559.01.T01 MTR_7g035295 28.634 227 112 7 65 275 173 365 4.67e-16 77.8
MsG0780038559.01.T01 MTR_7g066040 40.496 121 60 4 56 164 76 196 4.83e-16 75.9
MsG0780038559.01.T01 MTR_2g021155 25.994 327 160 15 1 264 54 361 7.31e-16 77.8
MsG0780038559.01.T01 MTR_7g066080 32.127 221 117 8 67 264 172 382 1.34e-15 76.6
MsG0780038559.01.T01 MTR_5g011350 38.168 131 57 5 67 190 125 238 5.28e-15 73.2
MsG0780038559.01.T01 MTR_1g097330 26.230 244 148 9 22 260 120 336 9.11e-15 73.9
MsG0780038559.01.T01 MTR_7g056023 35.862 145 73 6 67 201 143 277 5.10e-14 70.9
MsG0780038559.01.T01 MTR_4g053470 25.962 312 125 12 1 254 40 303 5.86e-14 71.2
MsG0780038559.01.T01 MTR_4g108820 25.940 266 148 8 1 236 60 306 7.15e-14 70.9
MsG0780038559.01.T01 MTR_1g050542 24.390 328 177 10 1 273 76 387 1.02e-13 70.9
MsG0780038559.01.T01 MTR_2g029990 26.690 281 130 11 1 217 69 337 1.27e-13 70.5
MsG0780038559.01.T01 MTR_3g007360 28.431 204 122 9 67 264 197 382 1.50e-13 70.5
MsG0780038559.01.T01 MTR_1g058190 24.138 319 172 13 5 273 57 355 1.59e-13 70.1
MsG0780038559.01.T01 MTR_7g078940 29.665 209 84 6 67 266 212 366 1.85e-13 70.1
MsG0780038559.01.T01 MTR_8g101580 24.498 249 159 7 30 275 159 381 3.14e-13 69.3
MsG0780038559.01.T01 MTR_7g056373 40.964 83 47 2 1 82 40 121 3.15e-13 65.5
MsG0780038559.01.T01 MTR_6g017270 38.318 107 56 2 178 275 221 326 3.49e-13 68.9
MsG0780038559.01.T01 MTR_3g009240 25.648 347 163 18 1 276 54 376 4.08e-13 68.9
MsG0780038559.01.T01 MTR_5g022420 28.780 205 130 8 67 271 164 352 5.36e-13 68.6
MsG0780038559.01.T01 MTR_3g007410 28.431 204 122 9 67 264 197 382 5.36e-13 68.9
MsG0780038559.01.T01 MTR_6g065980 29.032 217 113 12 67 273 155 340 9.36e-13 67.8
MsG0780038559.01.T01 MTR_6g004660 26.923 208 137 6 66 272 169 362 8.08e-12 65.1
MsG0780038559.01.T01 MTR_3g013740 27.670 206 129 9 67 266 197 388 1.27e-11 64.7
MsG0780038559.01.T01 MTR_0354s0030 43.210 81 38 2 203 275 1 81 1.33e-11 60.1
MsG0780038559.01.T01 MTR_7g078050 49.180 61 27 1 1 61 24 80 1.94e-11 63.2
MsG0780038559.01.T01 MTR_3g011020 25.792 221 129 12 67 273 160 359 1.98e-11 63.9
MsG0780038559.01.T01 MTR_8g062660 35.099 151 70 8 67 212 182 309 2.01e-11 64.3
MsG0780038559.01.T01 MTR_3g014540 26.415 212 117 9 67 264 171 357 2.57e-11 63.5
MsG0780038559.01.T01 MTR_4g023130 28.390 236 137 10 4 223 30 249 6.73e-11 62.0
MsG0780038559.01.T01 MTR_0064s0060 50.820 61 29 1 1 61 35 94 8.11e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 48 sgRNAs with CRISPR-Local

Find 62 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTATCATTGTTAAATTTA+AGG 0.066951 7:-48823976 None:intergenic
GAGAAAGTCTTCCCAATTAA+AGG 0.168120 7:-48823567 None:intergenic
ATAAGGTTGTGGCCTTTAAT+TGG 0.181231 7:+48823555 MsG0780038559.01.T01:CDS
CATATCTTATTGTTAATTCT+AGG 0.230427 7:-48824136 None:intergenic
ACAGAGCAATTCTCTATAAT+TGG 0.235735 7:+48824092 MsG0780038559.01.T01:CDS
GAACATCGTAAGTCTTTAAT+TGG 0.248331 7:+48823509 MsG0780038559.01.T01:CDS
TACATGACTATTGAGAAATA+TGG 0.257350 7:-48823425 None:intergenic
ATATGGAAGATGTCAAAATT+TGG 0.303991 7:+48823929 MsG0780038559.01.T01:CDS
CCATCATGCACTCTGGAATA+AGG 0.314904 7:-48823686 None:intergenic
ATTTCAGTTGCACCGTTAAC+TGG 0.326089 7:+48823714 MsG0780038559.01.T01:CDS
ATTAAAGGCCACAACCTTAT+AGG 0.326598 7:-48823552 None:intergenic
TCCATGCAAATGATCTATTA+AGG 0.328776 7:-48823400 None:intergenic
TAAGGATATATTAATTGATA+TGG 0.329647 7:-48823668 None:intergenic
TTAGTTTGGGTGATAATGTT+TGG 0.355432 7:+48823618 MsG0780038559.01.T01:CDS
AGAGAATTGCTCTGTCTTGT+AGG 0.357951 7:-48824084 None:intergenic
GACATATTCTCACAACTTTG+AGG 0.392348 7:-48823827 None:intergenic
TGATTCAACCAACACCTATA+AGG 0.394579 7:+48823538 MsG0780038559.01.T01:CDS
CAAATTTAGGATCGGAGATA+AGG 0.397015 7:-48823367 None:intergenic
GTTTCGCTTGATCTAAGTAC+TGG 0.400276 7:+48823782 MsG0780038559.01.T01:CDS
CATCTTCCATATAACAAAAT+CGG 0.408193 7:-48823918 None:intergenic
TAAGGTTGTGGCCTTTAATT+GGG 0.429599 7:+48823556 MsG0780038559.01.T01:CDS
AAATTTAGGATCGGAGATAA+GGG 0.433072 7:-48823366 None:intergenic
ACATATTCTCACAACTTTGA+GGG 0.468389 7:-48823826 None:intergenic
AAAGATGCAATGGGATTAAG+TGG 0.475689 7:-48824027 None:intergenic
GAGAATATGTCAGGTGCATA+TGG 0.476860 7:+48823839 MsG0780038559.01.T01:CDS
ACATGACTATTGAGAAATAT+GGG 0.477240 7:-48823424 None:intergenic
AGAGGGTGCCAACCAGTTAA+CGG 0.484726 7:-48823726 None:intergenic
AAAATGCTCATGTTCGCCTC+GGG 0.485945 7:-48823596 None:intergenic
CAAAGTTGTGAGAATATGTC+AGG 0.495583 7:+48823830 MsG0780038559.01.T01:CDS
AATTTGGAGATGACAAGTCT+TGG 0.501273 7:+48823945 MsG0780038559.01.T01:CDS
CAGTTGCACCGTTAACTGGT+TGG 0.527335 7:+48823718 MsG0780038559.01.T01:CDS
GGCCACAACCTTATAGGTGT+TGG 0.527688 7:-48823546 None:intergenic
ATTTCTCAATAGTCATGTAC+AGG 0.534902 7:+48823429 MsG0780038559.01.T01:CDS
TGAAGTGTGTATGTAAGTCT+TGG 0.539985 7:+48823339 MsG0780038559.01.T01:CDS
ACTGTAATCATCGGTTTCTG+TGG 0.554894 7:-48823456 None:intergenic
ACCTTAATAGATCATTTGCA+TGG 0.557606 7:+48823399 MsG0780038559.01.T01:CDS
CATATTCTCACAACTTTGAG+GGG 0.565813 7:-48823825 None:intergenic
AACCAACACCTATAAGGTTG+TGG 0.568524 7:+48823544 MsG0780038559.01.T01:CDS
ATGTACACCATCATGCACTC+TGG 0.571226 7:-48823693 None:intergenic
TTTCGCTTGATCTAAGTACT+GGG 0.574354 7:+48823783 MsG0780038559.01.T01:CDS
AATAAACTACTAACAGAGAA+GGG 0.575023 7:-48823485 None:intergenic
GGGTATGAAACTGTAATCAT+CGG 0.586270 7:-48823465 None:intergenic
TAATAAACTACTAACAGAGA+AGG 0.596384 7:-48823486 None:intergenic
CTGTAATCATCGGTTTCTGT+GGG 0.597587 7:-48823455 None:intergenic
CCTTATTCCAGAGTGCATGA+TGG 0.610318 7:+48823686 MsG0780038559.01.T01:CDS
ATATTCTCACAACTTTGAGG+GGG 0.669202 7:-48823824 None:intergenic
AAGATGCAATGGGATTAAGT+GGG 0.690108 7:-48824026 None:intergenic
AGACTTTCTCAGAAAACCCG+AGG 0.694153 7:+48823580 MsG0780038559.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CATATTATGATAAAAACAAA+AGG - Chr7:48823888-48823907 None:intergenic 15.0%
!! CATTATCATTGTTAAATTTA+AGG - Chr7:48823979-48823998 None:intergenic 15.0%
!! TAAGGATATATTAATTGATA+TGG - Chr7:48823671-48823690 None:intergenic 15.0%
!!! TTTTATCATAATATGATCAA+CGG + Chr7:48823893-48823912 MsG0780038559.01.T01:CDS 15.0%
!! CATATCTTATTGTTAATTCT+AGG - Chr7:48824139-48824158 None:intergenic 20.0%
!!! ATAGTTTTAAAAACACGATT+AGG - Chr7:48823758-48823777 None:intergenic 20.0%
!!! TATTTTGACAAATTTAGGAT+CGG - Chr7:48823378-48823397 None:intergenic 20.0%
! AATAAACTACTAACAGAGAA+GGG - Chr7:48823488-48823507 None:intergenic 25.0%
! ACATGACTATTGAGAAATAT+GGG - Chr7:48823427-48823446 None:intergenic 25.0%
! ATATGGAAGATGTCAAAATT+TGG + Chr7:48823929-48823948 MsG0780038559.01.T01:CDS 25.0%
! CATCTTCCATATAACAAAAT+CGG - Chr7:48823921-48823940 None:intergenic 25.0%
! TAATAAACTACTAACAGAGA+AGG - Chr7:48823489-48823508 None:intergenic 25.0%
! TACATGACTATTGAGAAATA+TGG - Chr7:48823428-48823447 None:intergenic 25.0%
!! CATTTTCAAAAAGATGCAAT+GGG - Chr7:48824039-48824058 None:intergenic 25.0%
!!! AACATGAGCATTTTTAGTTT+GGG + Chr7:48823605-48823624 MsG0780038559.01.T01:CDS 25.0%
!!! AGGTGTATTTTGACAAATTT+AGG - Chr7:48823383-48823402 None:intergenic 25.0%
ACAGAGCAATTCTCTATAAT+TGG + Chr7:48824092-48824111 MsG0780038559.01.T01:CDS 30.0%
ACATATTCTCACAACTTTGA+GGG - Chr7:48823829-48823848 None:intergenic 30.0%
ACCTTAATAGATCATTTGCA+TGG + Chr7:48823399-48823418 MsG0780038559.01.T01:CDS 30.0%
ATTTCTCAATAGTCATGTAC+AGG + Chr7:48823429-48823448 MsG0780038559.01.T01:CDS 30.0%
GAACATCGTAAGTCTTTAAT+TGG + Chr7:48823509-48823528 MsG0780038559.01.T01:CDS 30.0%
TCCATGCAAATGATCTATTA+AGG - Chr7:48823403-48823422 None:intergenic 30.0%
! AAATTTAGGATCGGAGATAA+GGG - Chr7:48823369-48823388 None:intergenic 30.0%
! CCATTTTCAAAAAGATGCAA+TGG - Chr7:48824040-48824059 None:intergenic 30.0%
! TTAGTTTGGGTGATAATGTT+TGG + Chr7:48823618-48823637 MsG0780038559.01.T01:CDS 30.0%
!! CCATTGCATCTTTTTGAAAA+TGG + Chr7:48824037-48824056 MsG0780038559.01.T01:CDS 30.0%
!!! GAACATGAGCATTTTTAGTT+TGG + Chr7:48823604-48823623 MsG0780038559.01.T01:CDS 30.0%
AAAGATGCAATGGGATTAAG+TGG - Chr7:48824030-48824049 None:intergenic 35.0%
AACACGATTAGGACAAAAAG+AGG - Chr7:48823747-48823766 None:intergenic 35.0%
AAGATGCAATGGGATTAAGT+GGG - Chr7:48824029-48824048 None:intergenic 35.0%
AATTTGGAGATGACAAGTCT+TGG + Chr7:48823945-48823964 MsG0780038559.01.T01:CDS 35.0%
ACACGATTAGGACAAAAAGA+GGG - Chr7:48823746-48823765 None:intergenic 35.0%
ATAAGGTTGTGGCCTTTAAT+TGG + Chr7:48823555-48823574 MsG0780038559.01.T01:CDS 35.0%
ATTAAAGGCCACAACCTTAT+AGG - Chr7:48823555-48823574 None:intergenic 35.0%
CAAAGTTGTGAGAATATGTC+AGG + Chr7:48823830-48823849 MsG0780038559.01.T01:CDS 35.0%
CATATTCTCACAACTTTGAG+GGG - Chr7:48823828-48823847 None:intergenic 35.0%
GACATATTCTCACAACTTTG+AGG - Chr7:48823830-48823849 None:intergenic 35.0%
GAGAAAGTCTTCCCAATTAA+AGG - Chr7:48823570-48823589 None:intergenic 35.0%
GGGTATGAAACTGTAATCAT+CGG - Chr7:48823468-48823487 None:intergenic 35.0%
TAAGGTTGTGGCCTTTAATT+GGG + Chr7:48823556-48823575 MsG0780038559.01.T01:CDS 35.0%
TGAAGTGTGTATGTAAGTCT+TGG + Chr7:48823339-48823358 MsG0780038559.01.T01:CDS 35.0%
TGATTCAACCAACACCTATA+AGG + Chr7:48823538-48823557 MsG0780038559.01.T01:CDS 35.0%
TTTCGCTTGATCTAAGTACT+GGG + Chr7:48823783-48823802 MsG0780038559.01.T01:CDS 35.0%
! ATATTCTCACAACTTTGAGG+GGG - Chr7:48823827-48823846 None:intergenic 35.0%
! CAAATTTAGGATCGGAGATA+AGG - Chr7:48823370-48823389 None:intergenic 35.0%
!! ACGGAACCGATTTTGTTATA+TGG + Chr7:48823912-48823931 MsG0780038559.01.T01:CDS 35.0%
AAAAATGCTCATGTTCGCCT+CGG - Chr7:48823600-48823619 None:intergenic 40.0%
AACCAACACCTATAAGGTTG+TGG + Chr7:48823544-48823563 MsG0780038559.01.T01:CDS 40.0%
AGAGAATTGCTCTGTCTTGT+AGG - Chr7:48824087-48824106 None:intergenic 40.0%
ATTTCAGTTGCACCGTTAAC+TGG + Chr7:48823714-48823733 MsG0780038559.01.T01:CDS 40.0%
GAGAATATGTCAGGTGCATA+TGG + Chr7:48823839-48823858 MsG0780038559.01.T01:CDS 40.0%
GTTTCGCTTGATCTAAGTAC+TGG + Chr7:48823782-48823801 MsG0780038559.01.T01:CDS 40.0%
!! ACTGTAATCATCGGTTTCTG+TGG - Chr7:48823459-48823478 None:intergenic 40.0%
!! CTGTAATCATCGGTTTCTGT+GGG - Chr7:48823458-48823477 None:intergenic 40.0%
AAAATGCTCATGTTCGCCTC+GGG - Chr7:48823599-48823618 None:intergenic 45.0%
AGACTTTCTCAGAAAACCCG+AGG + Chr7:48823580-48823599 MsG0780038559.01.T01:CDS 45.0%
ATGTACACCATCATGCACTC+TGG - Chr7:48823696-48823715 None:intergenic 45.0%
CCTTATTCCAGAGTGCATGA+TGG + Chr7:48823686-48823705 MsG0780038559.01.T01:CDS 45.0%
!! CCATCATGCACTCTGGAATA+AGG - Chr7:48823689-48823708 None:intergenic 45.0%
CAGTTGCACCGTTAACTGGT+TGG + Chr7:48823718-48823737 MsG0780038559.01.T01:CDS 50.0%
GGCCACAACCTTATAGGTGT+TGG - Chr7:48823549-48823568 None:intergenic 50.0%
!! AGAGGGTGCCAACCAGTTAA+CGG - Chr7:48823729-48823748 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
Chr7 gene 48823332 48824162 48823332 ID=MsG0780038559.01;Name=MsG0780038559.01
Chr7 mRNA 48823332 48824162 48823332 ID=MsG0780038559.01.T01;Parent=MsG0780038559.01;Name=MsG0780038559.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|276
Chr7 exon 48823332 48824162 48823332 ID=MsG0780038559.01.T01:exon:14515;Parent=MsG0780038559.01.T01
Chr7 CDS 48823332 48824162 48823332 ID=MsG0780038559.01.T01:cds;Parent=MsG0780038559.01.T01
Gene Sequence

>MsG0780038559.01.T01

ATGAAGATGAAGTGTGTATGTAAGTCTTGGAAAACCCTTATCTCCGATCCTAAATTTGTCAAAATACACCTTAATAGATCATTTGCATGGAACCCATATTTCTCAATAGTCATGTACAGGAGACCCACAGAAACCGATGATTACAGTTTCATACCCTTCTCTGTTAGTAGTTTATTAGAACATCGTAAGTCTTTAATTGGTTATGATGATTCAACCAACACCTATAAGGTTGTGGCCTTTAATTGGGAAGACTTTCTCAGAAAACCCGAGGCGAACATGAGCATTTTTAGTTTGGGTGATAATGTTTGGAGAAGTATTAAAAGTTTAGCTATTGTGCCATATCAATTAATATATCCTTATTCCAGAGTGCATGATGGTGTACATTTCAGTTGCACCGTTAACTGGTTGGCACCCTCTTTTTGTCCTAATCGTGTTTTTAAAACTATTATTGTTTCGCTTGATCTAAGTACTGGGACATACACACAGTTGATGCCCCCTCAAAGTTGTGAGAATATGTCAGGTGCATATGGAAGTGTTTGTGTGTTCATGAACTCCTTTTGTTTTTATCATAATATGATCAACGGAACCGATTTTGTTATATGGAAGATGTCAAAATTTGGAGATGACAAGTCTTGGACTCAATTCCTTAAATTTAACAATGATAATGTTCGAGTGAATTGTGAAATTAGATTTGCCCACTTAATCCCATTGCATCTTTTTGAAAATGGTGATACACTTTTATTCGCAAACAGCCTACAAGACAGAGCAATTCTCTATAATTGGAGAAAAAATATAGTATTGAAACCTAGAATTAACAATAAGATATGTTGA
Protein sequence

>MsG0780038559.01.T01

MKMKCVCKSWKTLISDPKFVKIHLNRSFAWNPYFSIVMYRRPTETDDYSFIPFSVSSLLEHRKSLIGYDDSTNTYKVVAFNWEDFLRKPEANMSIFSLGDNVWRSIKSLAIVPYQLIYPYSRVHDGVHFSCTVNWLAPSFCPNRVFKTIIVSLDLSTGTYTQLMPPQSCENMSGAYGSVCVFMNSFCFYHNMINGTDFVIWKMSKFGDDKSWTQFLKFNNDNVRVNCEIRFAHLIPLHLFENGDTLLFANSLQDRAILYNWRKNIVLKPRINNKIC*