AlfalfaGEDB | MsG0480018915.01

M. sativa cultivar ZhongmuNo.1 / MsG0480018915.01

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480018915.01.T01	AES87569.1	50.424	236	102	6	1	222	69	303	2.32E-61	208

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480018915.01.T01	G7JKQ3	50.424	236	102	6	1	222	69	303	1.11e-61	208

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
MsG0080047824.01	MsG0480018915.01	PPI
MsG0080048101.01	MsG0480018915.01	PPI
MsG0080048486.01	MsG0480018915.01	PPI
MsG0080048487.01	MsG0480018915.01	PPI
MsG0080048487.01	MsG0480018915.01	PPI
MsG0080048487.01	MsG0480018915.01	PPI
MsG0080048487.01	MsG0480018915.01	PPI
MsG0080048487.01	MsG0480018915.01	PPI
MsG0080048488.01	MsG0480018915.01	PPI
MsG0080048489.01	MsG0480018915.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480018915.01.T01	MTR_4g030110	50.424	236	102	6	1	222	69	303	2.81e-65	208
MsG0480018915.01.T01	MTR_3g464470	48.291	234	99	5	1	222	46	269	5.90e-62	199
MsG0480018915.01.T01	MTR_4g030830	46.639	238	106	9	1	222	48	280	1.38e-58	191
MsG0480018915.01.T01	MTR_3g464500	46.639	238	100	6	1	222	66	292	3.96e-58	190
MsG0480018915.01.T01	MTR_4g030980	43.774	265	103	7	1	221	54	316	4.15e-58	191
MsG0480018915.01.T01	MTR_4g031020	43.233	266	105	7	1	222	54	317	2.44e-57	189
MsG0480018915.01.T01	MTR_4g019990	45.000	240	110	6	1	222	45	280	4.50e-57	187
MsG0480018915.01.T01	MTR_4g024890	45.339	236	104	11	1	222	49	273	3.70e-51	171
MsG0480018915.01.T01	MTR_1g112010	44.493	227	91	7	1	220	19	217	9.34e-51	169
MsG0480018915.01.T01	MTR_6g055210	41.841	239	115	8	2	220	62	296	1.14e-47	164

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 48 sgRNAs with CRISPR-Local

Find 206 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGTGGAATAACAGGGAA+AGG	0.552944	4:-11624147	None:intergenic
AAAATCAGATTTAGTCCTAG+TGG	0.586212	4:-11623959	None:intergenic
AACAATGGTGTTTATTTGAG+TGG	0.493766	4:+11624189	MsG0480018915.01.T01:CDS
AAGTTGAGTGTGTGTGTCAA+TGG	0.487692	4:-11624313	None:intergenic
ACAAGCTTTGGCGGATGACG+TGG	0.568789	4:-11624363	None:intergenic
ACGTCATCCGCCAAAGCTTG+TGG	0.543308	4:+11624365	MsG0480018915.01.T01:CDS
AGCTACCACCTTATAAGTTT+CGG	0.377876	4:-11624037	None:intergenic
ATCAACACCACAAGCTTTGG+CGG	0.500956	4:-11624372	None:intergenic
ATCATTTCCATGGATTGATC+AGG	0.448227	4:+11623851	MsG0480018915.01.T01:CDS
ATGCAATGGATTAATCTACT+TGG	0.481053	4:+11623903	MsG0480018915.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAATCAGATTTAGTCCTAG+TGG	-	Chr4:11623962-11623981	None:intergenic	30.0%
AACAATATAAACAGGCTAAG+AGG	-	Chr4:11624834-11624853	None:intergenic	30.0%
AACAATGGTGTTTATTTGAG+TGG	+	Chr4:11624189-11624208	MsG0480018915.01.T01:CDS	30.0%
AACATGGTAACTTGAAATTC+TGG	+	Chr4:11626545-11626564	MsG0480018915.01.T01:intron	30.0%
ACAAACACAAATCATCTTAG+TGG	+	Chr4:11626008-11626027	MsG0480018915.01.T01:intron	30.0%
AGTTATCATTTGATCAGTGA+TGG	-	Chr4:11624985-11625004	None:intergenic	30.0%
ATGGAAATGATCATAGTGAA+TGG	-	Chr4:11623842-11623861	None:intergenic	30.0%
ATTCACTATGATCATTTCCA+TGG	+	Chr4:11623841-11623860	MsG0480018915.01.T01:CDS	30.0%
CATCAAAACAAATTCCATGA+TGG	+	Chr4:11626904-11626923	MsG0480018915.01.T01:intron	30.0%
CATCACGTTTATTATTTCCT+TGG	-	Chr4:11625228-11625247	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	11623724	11627587	11623724	ID=MsG0480018915.01;Name=MsG0480018915.01
Chr4	mRNA	11623724	11627587	11623724	ID=MsG0480018915.01.T01;Parent=MsG0480018915.01;Name=MsG0480018915.01.T01;_AED=0.40;_eAED=0.40;_QI=0\|0\|0\|1\|1\|1\|2\|0\|257
Chr4	exon	11623724	11624386	11623724	ID=MsG0480018915.01.T01:exon:7284;Parent=MsG0480018915.01.T01
Chr4	exon	11627477	11627587	11627477	ID=MsG0480018915.01.T01:exon:7285;Parent=MsG0480018915.01.T01
Chr4	CDS	11623724	11624386	11623724	ID=MsG0480018915.01.T01:cds;Parent=MsG0480018915.01.T01
Chr4	CDS	11627477	11627587	11627477	ID=MsG0480018915.01.T01:cds;Parent=MsG0480018915.01.T01

Gene Sequence

>MsG0480018915.01.T01

ATGCATCTCGAGAAATCAACACGAAACCCGCACCTAGCTCTAACTTTGCCACCAATGTTTGATGTCATTTGTACCATCCCAGTTTCTCGTGTACTCGATAATTCATGGACGACCACCATTCACTATGATCATTTCCATGGATTGATCAGGAATGAAACTATTTGGATGGTTGCTGGTTCATGCAATGGATTAATCTACTTGGTTTATATTTATACTCTCCGTTTTTGGAACCCAGCCACTAGGACTAAATCTGATTTTATTTCAGTTTCTTTGAATGAATCTTTTCAGCACTCTTTTGGTTATGATACTTTGACCGAAACTTATAAGGTGGTAGCTCTCCGTATAGCGAAGGAGCATGGCAATGCAGCAAGAAGCATTGTGCAAATTTTCACTTTGGGGGATAATTCTTGGAGAAATATTCATCCTTTCCCTGTTATTCCACTTTTATGGGCTTATCATTATTATAACAATGGTGTTTATTTGAGTGGTACTATTAATTGGTTGGCTGTCAGCGATTACTTTGGTTCTGATTTTCTACGTGCGCGTGATGTTACGATTGATCAATATGTGATTCTTTCACTTCATCTCTCCATTGACACACACACTCAACTTTTGCTGCCTCAGGGCTTTAATAAGCTGCCACGTCATCCGCCAAAGCTTGTGTTATGTGCTTCTGAAAAGGATGTTATTGCTTTAAATGAGGAAATGCAAAATCTGCCAAATGCAACTGCTTTAGATGATGATGAAGTGGAAATTCTACTTGATCCACATTGA

Protein sequence

>MsG0480018915.01.T01

MHLEKSTRNPHLALTLPPMFDVICTIPVSRVLDNSWTTTIHYDHFHGLIRNETIWMVAGSCNGLIYLVYIYTLRFWNPATRTKSDFISVSLNESFQHSFGYDTLTETYKVVALRIAKEHGNAARSIVQIFTLGDNSWRNIHPFPVIPLLWAYHYYNNGVYLSGTINWLAVSDYFGSDFLRARDVTIDQYVILSLHLSIDTHTQLLLPQGFNKLPRHPPKLVLCASEKDVIALNEEMQNLPNATALDDDEVEILLDPH*